We are delighted to announce #time_resolved_23 Find out more and register through https://timeresolved23.le.ac.uk/
*Dynamic Crystallography - XFELs and Synchrotrons to study enzyme reactions 13-15 September, Leicester, UK* The meeting is sponsored by the UK’s BBSRC Japan Partnering Award Scheme, the meeting aims to bring researchers from Japan who are developing and using techniques to exploit XFELs and synchrotrons for time-resolved studies of enzyme mechanisms together with researchers from the UK and our partners with similar interests to present and discuss prospects, progress and, of course, results. All with a view to establishing and furthering collaborations. The current planned outline of the meeting/workshop is: (see the meeting website timeresolved23.le.ac.uk for updates) *Thursday 14 September * *Preparation* – How to make suitable samples to enable the study of transient or short-lived enzyme reaction stats *Validation* – How to monitor reactions in crystals *Data Collection* – Strategies and protocols for the collection of diffraction and spectroscopic data *Friday 15 September * *Data Processing* – Strategies, and protocols for the optimal recovery of high-quality data *Analysis and interpretation* – How to overcome temporal uncertainty with respect to reaction coordinates and how to interpret mixed populations. *Results *– Examples of the application of dynamic investigation of protein mechanisms using structural and spectroscopic techniques. At the time of writing we have the following colleagues from Japan who will participate (with titles of contributions where we have them): - - * Hiroshi Sugimoto* (SACLA Spring-8/RIKEN) “Dynamics of hydrogen atom revealed by ultra-high resolution structure of the heme acquisition protein” - *Takehiko Tosha* (University of Hyogo) “Characterization of the short-lived reaction intermediate of NO reductase with caged substrate” - *Yoshitsugu Shiro* (University of Hyogo) - *Hitomi Sawai* (University of Nakasaki) - *Michihiro Suga* (Okayama University) - *Minoru Kubo* (University of Hyogo) “Time resolved spectroscopy for monitoring the protein dynamics in microcrystals” - *Tetsuo Katayama (Japan Synchrotron Radiation Research Institute) *"Instrumental development and data analysis of X-ray emission spectroscopy supporting SFX at SACLA" - * Tetsunari Kimura* (Kobe University) “Development of the microfluidic mixer for time-resolved SFX and spectroscopy” Speakers from Europe we currently have confirmed: - * Allen Orville* (XFEL Hub at Diamond Light Source) - * Ivo Tews* (University of Southampton) - * Mike Hough* (Diamond Light Source)*Dr Briony Yorke* (University of Leeds) "Developing multiplexing methods for time-resolved crystallographic experiments" - *Robin Owen* (Diamond Light Source) “Efficient sample delivery for time resolved SSX and SFX” (TBC) - *Thomas Ursby *(MAX IV Laboratory) "Serial Crystallography at MicroMAX - Flexibility offering new possibilities" - *Emily Freeman* (University of Oxford) “Time-resolved macromolecular crystallography studies of AmpC from *Escherichia coli* using synchrotron and XFEL radiation”. - *Jack Stubbs* (University of Southampton and Diamond Light Source) "Size matters: Microfluidics for defining protein crystal dimensions and rapid micromixing for time-resolved serial crystallography" - *Patrick Shaw Stewart *(Douglas Instruments) "Preparing crystal samples for serial data collection and microED: using microseeding and phase diagrams with microbatch-under-oil" - *James Beilsten- Edmands* (Diamond Light Source) “Processing serial diffraction data with DIALS & xia2”. - *Marcus Gallagher-Jones* (Rosalind Franklin Institute) - *Thomas Barends* (MPI-IMF Heidelberg) Please don't use "reply to" with this email, if you want to get in touch with me use my university email address peter.moody at le.ac.uk ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/