Re: [ccp4bb] cyseteine modification
Dear Sir, Thank you all who have replied. I jast want to enquire that if there is any option in coot to introduce the OH bond with Cys residue as well as to introduce the secondary peptide like bond between carboxylic carbon with amino group of lys. Sincerely Debajyoti Dutta On Wed, 19 Aug 2009 20:58:44 +0530 wrote Dear Debajyoti There is also the sulfenic acid species (-S-OH) which is actually the first oxidized form of sulfhydryls on the way to sulfonic acid. However sulfenic acids are very susceptible to further oxidation to sulfinic and sulphonic acids, and therefore need a protective chemical environment to remain stable. See for example some previous work of ours on sulfenic and sulfinic forms of active-site cysteines in glutathione reductase (Nature Structure Biology vol 5, 267-271, 1998) and the corrresponding pdb entries 1dnc and 1gsn. Best regards Savvas Savvas Savvides L-ProBE, Unit for Structural Biology Ghent University K.L. Ledeganckstraat 35 9000 Ghent, BELGIUM office: +32-(0)9-264.51.24 ; mobile: +32-(0)472-92.85.19 Email: savvas.savvi...@ugent.be http://www.lprobe.ugent.be/xray.html From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Debajyoti Dutta Sent: Wednesday, August 19, 2009 4:42 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] cyseteine modification Dear Sir, Is there any other oxidation states of cysteine other than cysteine sulphinic acid and cysteine sulphonic acid. In my protein, the cysteine molecule is definitely overoxidized but the electron density is not corresponding to the sulphinic or the sulphonic acid. The positive density looks as if it can accomodate only one oxygen atom and not more. Thank you for reply in advance. Sincerely Debajyoti Dutta E-mail message checked by Spyware Doctor (6.1.0.447)Database version: 6.13080http://www.pctools.com/spyware-doctor-antivirus/
[ccp4bb] cyseteine modification
Dear Sir, Is there any other oxidation states of cysteine other than cysteine sulphinic acid and cysteine sulphonic acid. In my protein, the cysteine molecule is definitely overoxidized but the electron density is not corresponding to the sulphinic or the sulphonic acid. The positive density looks as if it can accomodate only one oxygen atom and not more. Thank you for reply in advance. Sincerely Debajyoti Dutta
Re: [ccp4bb] cyseteine modification
Dear Debajyoti There is also the sulfenic acid species (-S-OH) which is actually the first oxidized form of sulfhydryls on the way to sulfonic acid. However sulfenic acids are very susceptible to further oxidation to sulfinic and sulphonic acids, and therefore need a protective chemical environment to remain stable. See for example some previous work of ours on sulfenic and sulfinic forms of active-site cysteines in glutathione reductase (Nature Structure Biology vol 5, 267-271, 1998) and the corrresponding pdb entries 1dnc and 1gsn. Best regards Savvas Savvas Savvides L-ProBE, Unit for Structural Biology Ghent University K.L. Ledeganckstraat 35 9000 Ghent, BELGIUM office: +32-(0)9-264.51.24 ; mobile: +32-(0)472-92.85.19 Email: savvas.savvi...@ugent.be http://www.lprobe.ugent.be/xray.html From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Debajyoti Dutta Sent: Wednesday, August 19, 2009 4:42 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] cyseteine modification Dear Sir, Is there any other oxidation states of cysteine other than cysteine sulphinic acid and cysteine sulphonic acid. In my protein, the cysteine molecule is definitely overoxidized but the electron density is not corresponding to the sulphinic or the sulphonic acid. The positive density looks as if it can accomodate only one oxygen atom and not more. Thank you for reply in advance. Sincerely Debajyoti Dutta http://sigads.rediff.com/RealMedia/ads/click_nx.ads/www.rediffmail.com/sign atureline@middle? E-mail message checked by Spyware Doctor (6.1.0.447) Database version: 6.13080 http://www.pctools.com/en/spyware-doctor-antivirus/
Re: [ccp4bb] cyseteine modification
You can also have a look at Active site structural features for chemically modified forms of rhodanese. Gliubich F, Gazerro M, Zanotti G, Delbono S, Bombieri G, Berni R. J Biol Chem. 1996 Aug 30;271(35):21054-61. Best Roberto On 19 Aug 2009, at 15:41, Debajyoti Dutta wrote: Dear Sir, Is there any other oxidation states of cysteine other than cysteine sulphinic acid and cysteine sulphonic acid. In my protein, the cysteine molecule is definitely overoxidized but the electron density is not corresponding to the sulphinic or the sulphonic acid. The positive density looks as if it can accomodate only one oxygen atom and not more. Thank you for reply in advance. Sincerely Debajyoti Dutta
