Re: [ccp4bb] glass capillaries
The capillaries have already found a new owner ! Ulrike To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] glass capillaries
Dear all, is anyone still using glass capillaries for crstal mounting at room temperature ??? We have a couple of them in size from 0.2 - 2.0 mm diameter and 80 mm length. I would be happy to give them away. Best, Ulrike To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
Re: [ccp4bb] Glass Capillaries
Dear Michael, I work with capillaries in a regular bases to grow crystal and use them for RT data collection or cryogenic temperature data collection at home source or at synchrotron sources. I like better borosilicate glass capillaries from Triana (http://www.trianatech.com/), as Patrick has already mentioned, for the cylindrical type but if I need capillaries with a reservoir them I go for the standard power diffraction capillaries from http://www.capillarytubes.co.uk/acatalog/Borosilicate_Glass_Capillary_Tubes.htmlwhich are similar to those from Hampton ( http://www.capillarytubes.co.uk/acatalog/Borosilicate_Glass_Capillary_Tubes.html), etc. All of them will give you some background but any of them will affect dramatically your data quality. You can do a search in ActaD or F and get good inputs on that (=*367 articles match your search capillaries*). On my hands borosilicate capillaries are easier to handle than Quartz capillaries and probably cheaper. Gavi. Dr. José A. Gavira Gallardo Laboratorio de Estudios Cristalográficos IACT, (CSIC-UGR) Av. de las Palmeras, 4 18100 Armilla (Granada) Tel.: 958 23 Ext. 19 01 06 Fax: 958 55 26 20 e-mail: jgav...@iact.ugr-csic.es or g...@lec.csic.es web: http://lec.ugr.es/~gavi/ 2012/11/12 Michael Roberts mrobert...@talktalk.net Dear All, I would be interested to learn of other crystallographers' experience in their use of glass capillaries for protein crystal growth and X-ray diffraction clarity. There are many types of glass available - quartz, soda glass, borosilicate, etc. Are there specific types which people prefer for best results overall? Best wishes, Michael Roberts
[ccp4bb] Glass Capillaries
Dear All, I would be interested to learn of other crystallographers' experience in their use of glass capillaries for protein crystal growth and X-ray diffraction clarity. There are many types of glass available - quartz, soda glass, borosilicate, etc. Are there specific types which people prefer for best results overall? Best wishes, Michael Roberts
Re: [ccp4bb] Glass Capillaries
Light glasses such as borosilicate. Can be purchased from Hampton research. FF Dr Felix Frolow Professor of Structural Biology and Biotechnology, Department of Molecular Microbiology and Biotechnology Tel Aviv University 69978, Israel Acta Crystallographica F, co-editor e-mail: mbfro...@post.tau.ac.il Tel: ++972-3640-8723 Fax: ++972-3640-9407 Cellular: 0547 459 608 On Nov 12, 2012, at 18:13 , Michael Roberts mrobert...@talktalk.net wrote: Dear All, I would be interested to learn of other crystallographers' experience in their use of glass capillaries for protein crystal growth and X-ray diffraction clarity. There are many types of glass available - quartz, soda glass, borosilicate, etc. Are there specific types which people prefer for best results overall? Best wishes, Michael Roberts
Re: [ccp4bb] Glass Capillaries
Hi Michael, I would recommend an alternative http://www.mitegen.com/products/micrort/micrort.shtml Traditional capillary is a pain to handle, unless you have a rock sized crystal. Good luck, Nian Huang On Mon, Nov 12, 2012 at 11:13 AM, Michael Roberts mrobert...@talktalk.netwrote: Dear All, I would be interested to learn of other crystallographers' experience in their use of glass capillaries for protein crystal growth and X-ray diffraction clarity. There are many types of glass available - quartz, soda glass, borosilicate, etc. Are there specific types which people prefer for best results overall? Best wishes, Michael Roberts
Re: [ccp4bb] Glass Capillaries
Traditional crystallography is difficult to practice, unless you know mathematics, physics, chemistry, computing etc….. :-) If one need to make science with room temperature diffraction, there is know substitution to old type glass capillaries that can be properly sealed :-\ FF Dr Felix Frolow Professor of Structural Biology and Biotechnology, Department of Molecular Microbiology and Biotechnology Tel Aviv University 69978, Israel Acta Crystallographica F, co-editor e-mail: mbfro...@post.tau.ac.il Tel: ++972-3640-8723 Fax: ++972-3640-9407 Cellular: 0547 459 608 On Nov 12, 2012, at 19:00 , Nian Huang huangn...@gmail.com wrote: Hi Michael, I would recommend an alternative http://www.mitegen.com/products/micrort/micrort.shtml Traditional capillary is a pain to handle, unless you have a rock sized crystal. Good luck, Nian Huang On Mon, Nov 12, 2012 at 11:13 AM, Michael Roberts mrobert...@talktalk.net wrote: Dear All, I would be interested to learn of other crystallographers' experience in their use of glass capillaries for protein crystal growth and X-ray diffraction clarity. There are many types of glass available - quartz, soda glass, borosilicate, etc. Are there specific types which people prefer for best results overall? Best wishes, Michael Roberts
Re: [ccp4bb] Glass Capillaries
The Triana range of capillaries are very easy to use for screening etc On 12 November 2012 16:13, Michael Roberts mrobert...@talktalk.net wrote: Dear All, I would be interested to learn of other crystallographers' experience in their use of glass capillaries for protein crystal growth and X-ray diffraction clarity. There are many types of glass available - quartz, soda glass, borosilicate, etc. Are there specific types which people prefer for best results overall? Best wishes, Michael Roberts -- patr...@douglas.co.ukDouglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36
Re: [ccp4bb] Glass Capillaries
I apologise for typing in dark. That is why know substitute no :-\ Dr Felix Frolow Professor of Structural Biology and Biotechnology, Department of Molecular Microbiology and Biotechnology Tel Aviv University 69978, Israel Acta Crystallographica F, co-editor e-mail: mbfro...@post.tau.ac.il Tel: ++972-3640-8723 Fax: ++972-3640-9407 Cellular: 0547 459 608 On Nov 12, 2012, at 19:05 , Felix Frolow mbfro...@post.tau.ac.il wrote: Traditional crystallography is difficult to practice, unless you know mathematics, physics, chemistry, computing etc….. :-) If one need to make science with room temperature diffraction, there is know substitution to old type glass capillaries that can be properly sealed :-\ FF Dr Felix Frolow Professor of Structural Biology and Biotechnology, Department of Molecular Microbiology and Biotechnology Tel Aviv University 69978, Israel Acta Crystallographica F, co-editor e-mail: mbfro...@post.tau.ac.il Tel: ++972-3640-8723 Fax: ++972-3640-9407 Cellular: 0547 459 608 On Nov 12, 2012, at 19:00 , Nian Huang huangn...@gmail.com wrote: Hi Michael, I would recommend an alternative http://www.mitegen.com/products/micrort/micrort.shtml Traditional capillary is a pain to handle, unless you have a rock sized crystal. Good luck, Nian Huang On Mon, Nov 12, 2012 at 11:13 AM, Michael Roberts mrobert...@talktalk.net wrote: Dear All, I would be interested to learn of other crystallographers' experience in their use of glass capillaries for protein crystal growth and X-ray diffraction clarity. There are many types of glass available - quartz, soda glass, borosilicate, etc. Are there specific types which people prefer for best results overall? Best wishes, Michael Roberts
Re: [ccp4bb] Glass Capillaries
On Nov 12, 2012, at 8:13 AM, Michael Roberts mrobert...@talktalk.net wrote: Dear All, I would be interested to learn of other crystallographers' experience in their use of glass capillaries for protein crystal growth and X-ray diffraction clarity. There are many types of glass available - quartz, soda glass, borosilicate, etc. Are there specific types which people prefer for best results overall? Best wishes, Michael Roberts Glass capillaries can be reactive with crystals. Quartz tends to be more neutral and has the advantage of being easier to cut, etc. as well. It does however create a higher background scatter, so the signal to noise ratio of the data collected might suffer a wee bit.
