Hi Alex,
In my experience you just have to experiment with these parameters (in
however of a limited space that is afforded by your experimental set-up).
If you have the right tools you can slog through semifactorial or DoE-based
scouting of various parameters with relative ease. These parameters
typically include pH, salinity, detergent(s), and other variables (e.g. the
nature of the buffer and salt(s) - many cases exist where the 'standard'
choices do not work well).
Literature-based analogies do help on occasion. For example,
membrane-spanning p450-type proteins often prefer high salt, and often
would do better in sodium acetate as opposed to chloride. I've worked with
ion channels that preferred 3M NaCl, or 2M MgCl2 and other fairly weird
conditions...
Artem
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On Fri, Mar 1, 2019 at 7:27 AM Alex Perálvarez Marín
wrote:
> Dear all,
>
> any reference as a guide for selecting the appropriate salt and
> concentration for membrane proteins extraction buffer?
>
> Best,
>
> Alex
>
> --
> Alex Perálvarez-Marín, Ph.D.
> Centre d'Estudis en Biofísica / Unitat de Biofísica
> Edifici M
> Universitat Autònoma de Barcelona
> 08193 Cerdanyola del Vallés
> Barcelona
> Spain
> Phone: +34 93 581 4504
> FAX: +34 93 581 1907
> e-mail: aperalva...@gmail.com
> LinkedIn: es.linkedin.com/in/aperalvarez/
>
>
>
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