Re: [ccp4bb] mystery feature near a PLP substrate

[Avinash Punekar]

DMSO may fit nicely there.

Re: [ccp4bb] mystery feature near a PLP substrate

[Karolina Michalska]

Hi, 

The Lys-PLP adduct looks like a perfectly happy internal aldimine. I do
not see any indication of a covalent bond between PLP and the density in
question indicating any other intermediate. Which, to my knowledge,
would be rather hard to get accidentally. I do not know distances
between these fairly well resolved peaks in the 2Fo-Fc map but I am
guessing they would nicely accommodate solvent molecules. The peak
closer to PLP is weaker, maybe just another partly occupied solvent
molecule? Could the continuity of your difference map be coming from too
low contour level? 

Best, 

Karolina  

W dniu 2017-05-24 09:16, Eleanor Dodson napisał(a):

> Any ideas?

Re: [ccp4bb] mystery feature near a PLP substrate

[SR Bharath]

It is most likely amino acrylate, the intermediate in the reaction of PLP
with amino acids.

On Wed, 24 May 2017 at 22:44, Francis Reyes 
wrote:

> Fidget spinner?
>
> https://en.m.wikipedia.org/wiki/Fidget_spinner
>
> F
>
> On May 24, 2017, at 10:16 AM, Eleanor Dodson 
> wrote:
>
> Any ideas?
>
> 
>
>

Re: [ccp4bb] mystery feature near a PLP substrate

[Francis Reyes]

Fidget spinner?

https://en.m.wikipedia.org/wiki/Fidget_spinner

F

> On May 24, 2017, at 10:16 AM, Eleanor Dodson  
> wrote:
> 
> Any ideas?
> 

Re: [ccp4bb] mystery feature near a PLP substrate

[Carlos Pla Prats]

Have you tried modeling a fidget spinner there?

Carlos

On Wed, May 24, 2017 at 4:33 PM, Soisson, Stephen M <
stephen_sois...@merck.com> wrote:

> This looks suspiciously like Cartoon moose-head.  Is it possible that you
> have contamination in your buffers?  We once had this problem when using
> Canadian water.
>
>
>
> [image: Related image]
> <https://www.google.com/imgres?imgurl=http%3A%2F%2Fworldartsme.com%2Fimages%2Fmoose-face-clipart-1.jpg=http%3A%2F%2Fworldartsme.com%2Fgolden-anniversary-borders-clipart.html=uEQWdFqPx_AyTM=39d_O1it6NYRlM%3A=1=450=397=865=1580=0ahUKEwim4vW834jUAhVK4IMKHb8jD2IQxiAIFigB=c=1>
>
>
>
> Steve
>
>
>
>
>
>
>
> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of 
> *Eleanor
> Dodson
> *Sent:* Wednesday, May 24, 2017 10:16 AM
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* [ccp4bb] mystery feature near a PLP substrate
>
>
>
> Any ideas?
>
> Notice:  This e-mail message, together with any attachments, contains
> information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
> New Jersey, USA 07033), and/or its affiliates Direct contact information
> for affiliates is available at
> http://www.merck.com/contact/contacts.html) that may be confidential,
> proprietary copyrighted and/or legally privileged. It is intended solely
> for the use of the individual or entity named on this message. If you are
> not the intended recipient, and have received this message in error,
> please notify us immediately by reply e-mail and then delete it from
> your system.
>

Re: [ccp4bb] mystery feature near a PLP substrate

[Soisson, Stephen M]

This looks suspiciously like Cartoon moose-head.  Is it possible that you have 
contamination in your buffers?  We once had this problem when using Canadian 
water.

[Related 
image]<https://www.google.com/imgres?imgurl=http%3A%2F%2Fworldartsme.com%2Fimages%2Fmoose-face-clipart-1.jpg=http%3A%2F%2Fworldartsme.com%2Fgolden-anniversary-borders-clipart.html=uEQWdFqPx_AyTM=39d_O1it6NYRlM%3A=1=450=397=865=1580=0ahUKEwim4vW834jUAhVK4IMKHb8jD2IQxiAIFigB=c=1>

Steve



From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Eleanor 
Dodson
Sent: Wednesday, May 24, 2017 10:16 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] mystery feature near a PLP substrate

Any ideas?
Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.