Re: [ccp4bb] off topic:how to prevent DNA tetraplexe forming?
On Dec 20, 2012, at 8:16 PM, dengzq1987 dengzq1...@gmail.com wrote: Hi all, recently, i perform an EMSA experiment.when i prepared the DNA with a 5' poly(G)-tailed ,i find that it formed guanine tetraplexes .how can i prevent this happen,or has any method to disrupt the guanine tetraplexes structure? any suggestion is appreciation. dengzq Unless it has some other, more stable structure to compete it out, you will have this problem persist. Even GTP in solution will do this. Bill William G. Scott Professor Department of Chemistry and Biochemistry and The Center for the Molecular Biology of RNA 228 Sinsheimer Laboratories University of California at Santa Cruz Santa Cruz, California 95064 USA
[ccp4bb] off topic:how to prevent DNA tetraplexe forming?
Hi all, recently, i perform an EMSA experiment.when i prepared the DNA with a 5' poly(G)-tailed ,i find that it formed guanine tetraplexes .how can i prevent this happen,or has any method to disrupt the guanine tetraplexes structure? any suggestion is appreciation. dengzq
Re: [ccp4bb] off topic:how to prevent DNA tetraplexe forming?
El 21/12/12 05:16, dengzq1987 escribió: Hi all, recently, i perform an EMSA experiment.when i prepared the DNA with a 5' poly(G)-tailed ,i find that it formed guanine tetraplexes .how can i prevent this happen,or has any method to disrupt the guanine tetraplexes structure? any suggestion is appreciation. dengzq Hi, How you know the tail forms tetraplexes? Couldn't be other type of structure? Tetraplexes are stabilized by potassium and, to a lesser extent by sodium cations. Try to avoid them in your buffer or to keep them to a minimum. Or you can use lithium as counterion, it is too small to stabilize the tetraplexes. Another option is to add a complementary poly(C) oligo before running your EMSA. Cheers, -- Miguel Architecture et Fonction des Macromolécules Biologiques (UMR7257) CNRS, Aix-Marseille Université Case 932, 163 Avenue de Luminy, 13288 Marseille cedex 9, France Tel: +33(0) 491 82 55 93 Fax: +33(0) 491 26 67 20 mailto:miguel.ortiz-lombar...@afmb.univ-mrs.fr http://www.afmb.univ-mrs.fr/Miguel-Ortiz-Lombardia
