Re: [ccp4bb] Today ...
Hi Juergen, Your scheme as printed has two J's - so January and July are indistinguishable! I would suggest the letters should instead be JFMAYULGSOND. Happy apocalypse! Tom On 21 Dec 2012, at 01:52, Bosch, Juergen jubo...@jhsph.edu wrote: May I introduce you to another fool proof way: 12d12 year-month-day This is in particular useful in a mixed lab with american and europeans labeling Eppendorf tubes differently. The month has a defined letter, like this you know that your buffer was made on June 9th and not September 6th J, F,M,A,Y,J,U,G,S,O,N,D for the months, simply first letter, but if taken move to the second letter etc. I'm sure you guys know about the two Bohemian mathematicians who figured out that the Apocalypse as predicted by the Mayan calendar is due in 104 years because the alignment of events was done incorrectly. Jürgen .. Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu On Dec 20, 2012, at 12:17 PM, David Schuller wrote: On 12/20/12 11:23, Edward A. Berry wrote: No, No- in scientific circles we go from MSB on the left to LSB on the right: 2012 12 20 (still a sort of palindrome). This is the best method if you are going to incorporate the date into a file name. That way alphanumeric and chronological searches come up the same. It's a pity it took us so long to figure this out, and the world is ending tomorrow. http://www.makemeacocktail.com/cocktail/7209/mayan-apocalypse/ Maya Apocalypse cocktail -- === All Things Serve the Beam === David J. Schuller modern man in a post-modern world MacCHESS, Cornell University schul...@cornell.edu
Re: [ccp4bb] Today ...
Tom Murray-Rust tom.murray.r...@gmail.com writes: Hi Juergen, Your scheme as printed has two J's - so January and July are indistinguishable! I would suggest the letters should instead be JFMAYULGSOND. Happy apocalypse! Tom On 21 Dec 2012, at 01:52, Bosch, Juergen jubo...@jhsph.edu wrote: May I introduce you to another fool proof way: 12d12 year-month-day This is in particular useful in a mixed lab with american and europeans labeling Eppendorf tubes differently. The month has a defined letter, like this you know that your buffer was made on June 9th and not September 6th J, F,M,A,Y,J,U,G,S,O,N,D for the months, simply first letter, but if taken move to the second letter etc. Or there’s the Japanese system, using characters which unambiguously identify which field is which. Happy 21日12月24年! I'm sure you guys know about the two Bohemian mathematicians who figured out that the Apocalypse as predicted by the Mayan calendar is due in 104 years because the alignment of events was done incorrectly. Will there be egg on their faces in 104 year’s time! Wishing everyone a Merry Christmas, Happy New Year, and a sober instructive CCP4. -- Ian ◎
Re: [ccp4bb] Today ...
On neither case does an alphanumeric sort coincide with a chronological sort. The obvious solution is to petition to have the months renamed alphabetically. On 12/21/12 03:23, Tom Murray-Rust wrote: Hi Juergen, Your scheme as printed has two J's - so January and July are indistinguishable! I would suggest the letters should instead be JFMAYULGSOND. On 21 Dec 2012, at 01:52, Bosch, Juergen jubo...@jhsph.edu mailto:jubo...@jhsph.edu wrote: May I introduce you to another fool proof way: 12d12 ... J, F,M,A,Y,J,U,G,S,O,N,D for the months, simply first letter, but if taken move to the second letter etc. -- === All Things Serve the Beam === David J. Schuller modern man in a post-modern world MacCHESS, Cornell University schul...@cornell.edu
Re: [ccp4bb] Today ...
Hi David, on a computer I agree 20121221 is the way to go (but even then you could still do ls -lta), but on Eppendorf tubes that is precious real estate for other important numbers or letters hence the 12d21 :-) And thanks for catching my double J this was just a test if somebody would actually read what I wrote :-) And the corrected values were indeed right, aka following the simple rule I mentioned. Jürgen On Dec 21, 2012, at 9:15 AM, David Schuller wrote: On neither case does an alphanumeric sort coincide with a chronological sort. The obvious solution is to petition to have the months renamed alphabetically. On 12/21/12 03:23, Tom Murray-Rust wrote: Hi Juergen, Your scheme as printed has two J's - so January and July are indistinguishable! I would suggest the letters should instead be JFMAYULGSOND. On 21 Dec 2012, at 01:52, Bosch, Juergen jubo...@jhsph.edumailto:jubo...@jhsph.edu wrote: May I introduce you to another fool proof way: 12d12 ... J, F,M,A,Y,J,U,G,S,O,N,D for the months, simply first letter, but if taken move to the second letter etc. -- === All Things Serve the Beam === David J. Schuller modern man in a post-modern world MacCHESS, Cornell University schul...@cornell.edumailto:schul...@cornell.edu .. Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu
Re: [ccp4bb] Yesterday (was) Today yesterday...
Hi all Since this is a discussion by protein crystallographers about a possible standard, I will expect a consensus (or several conflicting consensuses*) to be reached sometime after I have retired... I'll look forward to the discussions spilling out into the CCP4 Study Weekend. * Since consensus was originally a fourth (not second) declension Latin noun, the nominative and accusative plurals are also consensus (but with a long u). Consensi is just wrong... Have a nice break! On 21 Dec 2012, at 14:45, Bosch, Juergen wrote: Hi David, on a computer I agree 20121221 is the way to go (but even then you could still do ls -lta), but on Eppendorf tubes that is precious real estate for other important numbers or letters hence the 12d21 :-) And thanks for catching my double J this was just a test if somebody would actually read what I wrote :-) And the corrected values were indeed right, aka following the simple rule I mentioned. Jürgen On Dec 21, 2012, at 9:15 AM, David Schuller wrote: On neither case does an alphanumeric sort coincide with a chronological sort. The obvious solution is to petition to have the months renamed alphabetically. On 12/21/12 03:23, Tom Murray-Rust wrote: Hi Juergen, Your scheme as printed has two J's - so January and July are indistinguishable! I would suggest the letters should instead be JFMAYULGSOND. On 21 Dec 2012, at 01:52, Bosch, Juergen jubo...@jhsph.edu wrote: May I introduce you to another fool proof way: 12d12 ... J, F,M,A,Y,J,U,G,S,O,N,D for the months, simply first letter, but if taken move to the second letter etc. -- === All Things Serve the Beam === David J. Schuller modern man in a post-modern world MacCHESS, Cornell University schul...@cornell.edu .. Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, MRC Centre, Hills Road, Cambridge, CB2 0QH Chairman of European Crystallographic Association SIG9 (Crystallographic Computing)
Re: [ccp4bb] off topic:how to prevent DNA tetraplexe forming?
On Dec 20, 2012, at 8:16 PM, dengzq1987 dengzq1...@gmail.com wrote: Hi all, recently, i perform an EMSA experiment.when i prepared the DNA with a 5' poly(G)-tailed ,i find that it formed guanine tetraplexes .how can i prevent this happen,or has any method to disrupt the guanine tetraplexes structure? any suggestion is appreciation. dengzq Unless it has some other, more stable structure to compete it out, you will have this problem persist. Even GTP in solution will do this. Bill William G. Scott Professor Department of Chemistry and Biochemistry and The Center for the Molecular Biology of RNA 228 Sinsheimer Laboratories University of California at Santa Cruz Santa Cruz, California 95064 USA
[ccp4bb] unsubscribe
-- *** Dr Diego M.A. Guerin Unidad de Biofisica (CSIC-UPV/EHU) - Fundacion Biofisica Bizkaia Barrio Sarriena S/N, 48940, Leioa, Vizcaya, Spain E-mail: diego.gue...@ehu.es http://www.ehu.es/biofisica http://sici.umh.es http://www.redtrv.org Tel: +34 94 601 3345 Fax: +34 94 601 3360 ADVERTENCE: The email and any files attached with this email are confidential. Any unauthorised dissemination or any use or disclosure of any information contained in them, is strictly prohibited and may be illegal. Please let me know if you received this message in error, and delete it from your computer. Thank you. ***
[ccp4bb] CCP4 6.3.0 update 012
Dear CCP4 Users, A CCP4 update, the last one in 2012, has just been released, consisting of the following changes: * QtRView: Stability fixes and improved user experience of dynamically changing report pages * CCP4 Update: Technical modifications to ccp4-6.3.0 in order to complete the changes introduced in update No 11 * Crank: Bug fixes in module mapro.exe (Windows only) If you do not currently receive updates, consider re-installing your CCP4 setup using latest binary packages, which now have CCP4 Update manager (ccp4um) integrated. Note that auto-updates will work correctly only with CCP4 release 6.3.0, therefore upgrade if necessary. Please report any bugs to c...@stfc.ac.ukmailto:c...@stfc.ac.uk. Many thanks for using CCP4. CCP4 Core Team wishes you a very Merry Christmas and Happy New Year 2013! [cid:BC01B0FF-48A9-4F73-B598-537FAD429F22@rc-harwell.ac.uk] Eugene Krissinel Andrey Lebedev -- Scanned by iCritical.
[ccp4bb] Scientist position available at SLS
http://www.psi.ch/pa/offenestellen/0491-1 Crystallization Facility Scientist Your tasks You will lead a joint facility between the MX-Group at the Swiss Light Source (SLS) and the BMR-Group at the Department of Biology and Chemistry, and build a high-throughput crystallisation platform. The facility aims at providing professional crystallisation services with 'standard' and lipidic cubic phase methods at 4 and 20 ºC. In collaboration with scientists and engineers of the MX-Group you will further develop the available in situ X-ray diffraction screening capabilities. In addition, you will have the opportunity to participate in the development of protein micro-diffraction to exploit the synchrotron-based micro-focus X-ray beam as well as X-ray Free Electron Laser (FEL) capabilities of the Paul Scherrer Institute. Your profile You hold a PhD degree in biology or chemistry, and have significant experience in crystallisation methods and protein crystallography. Practical knowledge in synchrotron related research and in project management is of advantage. If you are self-motivated, flexible, and a good team player this position will offer great opportunities to establish your research career in an exciting and highly multidisciplinary environment. For further information please contact Dr Meitian Wang, phone +41 56 310 41 75, Dr Vincent Olieric, phone +41 56 310 52 33 or Prof. Dr Michel Steinmetz, phone +41 56 310 47 54. Please submit your application online (including list of publications and addresses of referees) for the position as a Crystallization Facility Scientist (index no. 6112-03). Paul Scherrer Institut, Human Resources Management, Elke Baumann, 5232 Villigen PSI, Switzerland __ Meitian Wang Swiss Light Source at Paul Scherrer Institut CH-5232 Villigen PSI - http://www.psi.ch/sls/ Phone: +41 56 310 4175
