TEKTRAN RECEPTOR BINDING SITE-DELETED FOOT-AND-MOUTH DISEASE (FMD) VIRUS PROTECTS CATTLE FROM FMD Author(s): MCKENNA THOMAS S LUBROTH JUAN RIEDER ROJAS AIDA E BAXT BARRY MASON PETER W Interpretive Summary: Foot-and-mouth disease virus (FMDV) causes a devastating disease of domestic animals which can be controlled, under some circumstances, with inactivated vaccines. However, these vaccines must be produced by chemical inactivation of huge amounts of highly infectious virus, and the fact that commercial vaccines and vaccine production plants have been proven to be sources of outbreaks of the disease have contributed to the decision by the US to outlaw the production and use of this type of vaccine in the US. To overcome the safety problems associated with the use of an animal-infectious virus as the starting material for these vaccines, we have genetically engineered FMDV that are incapable of binding to or infecting cells grown in tissue culture. In this report we document the engineering of these receptor binding site-deleted viruses, and demonstrate that they are non-infectious in mice, swine, and cattle. Furthermore, we demonstrate that cattle inoculated with these "non-infectious" viruses produce a strong immune response to the natural virus. Finally, we demonstrate that these animals are protected from disease when exposed to live virus, demonstrating that these receptor binding site-deleted viruses are viable candidates for new FMD vaccines. Keywords: virology aphthovirus disease xray crystallography nmr recombinant dna receptor Contact: P O BOX 848 GREENPORT LI ORIENT POINT NY 11944 FAX: 516-323-2507 Email: Approved Date: 1995-10-23 TEKTRAN United States Department of Agriculture Agricultural Research Service Updated: 1998-12-18
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