Re: [Felvtalk] ELISA IFA Tests:
On 02-06, Lynda Wilson wrote: I've never heard of a PSA. Is this new? L I thought PSA was a prostate check for men. Lorrie ___ Felvtalk mailing list Felvtalk@felineleukemia.org http://felineleukemia.org/mailman/listinfo/felvtalk_felineleukemia.org
Re: [Felvtalk] ELISA IFA Tests:
That's what I found when I googled it, Lorrie :) This was in response to Kathryns thread. She said she meant a PCR test. I've never heard of this either. - Original Message - From: Lorrie felineres...@frontier.com To: felvtalk@felineleukemia.org Sent: Tuesday, February 07, 2012 5:40 AM Subject: Re: [Felvtalk] ELISA IFA Tests: On 02-06, Lynda Wilson wrote: I've never heard of a PSA. Is this new? L I thought PSA was a prostate check for men. Lorrie ___ Felvtalk mailing list Felvtalk@felineleukemia.org http://felineleukemia.org/mailman/listinfo/felvtalk_felineleukemia.org ___ Felvtalk mailing list Felvtalk@felineleukemia.org http://felineleukemia.org/mailman/listinfo/felvtalk_felineleukemia.org
Re: [Felvtalk] ELISA IFA Tests:
Hi, I am new to all of this. My cat Oliver had the PCR after testing positive with the ELISA. Sadly, he tested positive after getting the PCR test. He is a 6 year old Siamese. My vet had a chat with some hoity toity vet either at Davis or Cornell who said that it was more accurate. Everyone else in the house tested negative and just had their booster shots. Follow up blood work for all is in Mid March. Thanks for all of the info here! Holly -Original Message- From: Lynda Wilson longhornf...@verizon.net To: felvtalk felvtalk@felineleukemia.org Sent: Tue, Feb 7, 2012 6:02 am Subject: Re: [Felvtalk] ELISA IFA Tests: That's what I found when I googled it, Lorrie :) This was in response to Kathryns thread. She said she meant a PCR test. I've never heard of this either. - Original Message - From: Lorrie felineres...@frontier.com To: felvtalk@felineleukemia.org Sent: Tuesday, February 07, 2012 5:40 AM Subject: Re: [Felvtalk] ELISA IFA Tests: On 02-06, Lynda Wilson wrote: I've never heard of a PSA. Is this new? L I thought PSA was a prostate check for men. Lorrie ___ Felvtalk mailing list Felvtalk@felineleukemia.org http://felineleukemia.org/mailman/listinfo/felvtalk_felineleukemia.org ___ Felvtalk mailing list Felvtalk@felineleukemia.org http://felineleukemia.org/mailman/listinfo/felvtalk_felineleukemia.org ___ Felvtalk mailing list Felvtalk@felineleukemia.org http://felineleukemia.org/mailman/listinfo/felvtalk_felineleukemia.org
Re: [Felvtalk] ELISA IFA Tests:
From what I know, there are four: In-house ELISA Lab ELISA Lab IFA PSA Is this correct? On Mon, Feb 6, 2012 at 4:28 AM, Lynda Wilson longhornf...@verizon.net wrote: First of all there are two commonly used types of FeLV tests. One is the in-office ELISA and the other is the laboratory run IFA test. Both types test for the FeLV protein p27. However, they detect the protein in different forms and detect infection at different stages. 1.) If a cat or kitten is ELISA positive, but healthy, an IFA test should be performed. Here is where the confusion begins. If the cat is IFA negative but ELISA positive there are two possibilities. One is a false positive ELISA test and the other is a stage of the disease is present at which the IFA test doesn't detect infection. Therefore the suspect cat or kitten should be isolated and retested in at least 6 weeks. If, at that time the cat is IFA positive, you know that the cat if infected. The IFA is 99% accurate. An IFA positive cat can be shedding virus and infect other cats and kittens. Remember to retest 6 weeks after an ELISA positive result, the cat's immune system can clear the virus especially in an adult cat (kittens and seniors have the most difficulty clearing the virus). This is what I have always been told by veterinarians and everyone on this FeLV thread. I have not heard any different. This info is true! Lynda ___ Felvtalk mailing list Felvtalk@felineleukemia.org http://felineleukemia.org/mailman/listinfo/felvtalk_felineleukemia.org -- Go Get a Life---Go Get a Shelter Animal! Ask your local animal pound to start saving over 90% of their intake by implementing the No Kill Equation: http://www.nokilladvocacycenter.org/ Legislate better animal pound conditions: http://www.rescue50.org ___ Felvtalk mailing list Felvtalk@felineleukemia.org http://felineleukemia.org/mailman/listinfo/felvtalk_felineleukemia.org
Re: [Felvtalk] ELISA IFA Tests:
I've never heard of a PSA. Is this new? L - Original Message - From: Kathryn Hargreaves khargrea...@gmail.com To: felvtalk@felineleukemia.org Sent: Monday, February 06, 2012 5:43 PM Subject: Re: [Felvtalk] ELISA IFA Tests: From what I know, there are four: In-house ELISA Lab ELISA Lab IFA PSA Is this correct? On Mon, Feb 6, 2012 at 4:28 AM, Lynda Wilson longhornf...@verizon.net wrote: First of all there are two commonly used types of FeLV tests. One is the in-office ELISA and the other is the laboratory run IFA test. Both types test for the FeLV protein p27. However, they detect the protein in different forms and detect infection at different stages. 1.) If a cat or kitten is ELISA positive, but healthy, an IFA test should be performed. Here is where the confusion begins. If the cat is IFA negative but ELISA positive there are two possibilities. One is a false positive ELISA test and the other is a stage of the disease is present at which the IFA test doesn't detect infection. Therefore the suspect cat or kitten should be isolated and retested in at least 6 weeks. If, at that time the cat is IFA positive, you know that the cat if infected. The IFA is 99% accurate. An IFA positive cat can be shedding virus and infect other cats and kittens. Remember to retest 6 weeks after an ELISA positive result, the cat's immune system can clear the virus especially in an adult cat (kittens and seniors have the most difficulty clearing the virus). This is what I have always been told by veterinarians and everyone on this FeLV thread. I have not heard any different. This info is true! Lynda ___ Felvtalk mailing list Felvtalk@felineleukemia.org http://felineleukemia.org/mailman/listinfo/felvtalk_felineleukemia.org -- Go Get a Life---Go Get a Shelter Animal! Ask your local animal pound to start saving over 90% of their intake by implementing the No Kill Equation: http://www.nokilladvocacycenter.org/ Legislate better animal pound conditions: http://www.rescue50.org ___ Felvtalk mailing list Felvtalk@felineleukemia.org http://felineleukemia.org/mailman/listinfo/felvtalk_felineleukemia.org ___ Felvtalk mailing list Felvtalk@felineleukemia.org http://felineleukemia.org/mailman/listinfo/felvtalk_felineleukemia.org
Re: [Felvtalk] ELISA IFA Tests:
I'm sorry: PCR. On Mon, Feb 6, 2012 at 7:11 PM, Lynda Wilson longhornf...@verizon.net wrote: I've never heard of a PSA. Is this new? L - Original Message - From: Kathryn Hargreaves khargrea...@gmail.com To: felvtalk@felineleukemia.org Sent: Monday, February 06, 2012 5:43 PM Subject: Re: [Felvtalk] ELISA IFA Tests: From what I know, there are four: In-house ELISA Lab ELISA Lab IFA PSA Is this correct? On Mon, Feb 6, 2012 at 4:28 AM, Lynda Wilson longhornf...@verizon.net wrote: First of all there are two commonly used types of FeLV tests. One is the in-office ELISA and the other is the laboratory run IFA test. Both types test for the FeLV protein p27. However, they detect the protein in different forms and detect infection at different stages. 1.) If a cat or kitten is ELISA positive, but healthy, an IFA test should be performed. Here is where the confusion begins. If the cat is IFA negative but ELISA positive there are two possibilities. One is a false positive ELISA test and the other is a stage of the disease is present at which the IFA test doesn't detect infection. Therefore the suspect cat or kitten should be isolated and retested in at least 6 weeks. If, at that time the cat is IFA positive, you know that the cat if infected. The IFA is 99% accurate. An IFA positive cat can be shedding virus and infect other cats and kittens. Remember to retest 6 weeks after an ELISA positive result, the cat's immune system can clear the virus especially in an adult cat (kittens and seniors have the most difficulty clearing the virus). This is what I have always been told by veterinarians and everyone on this FeLV thread. I have not heard any different. This info is true! Lynda ___ Felvtalk mailing list Felvtalk@felineleukemia.org http://felineleukemia.org/mailman/listinfo/felvtalk_felineleukemia.org -- Go Get a Life---Go Get a Shelter Animal! Ask your local animal pound to start saving over 90% of their intake by implementing the No Kill Equation: http://www.nokilladvocacycenter.org/ Legislate better animal pound conditions: http://www.rescue50.org ___ Felvtalk mailing list Felvtalk@felineleukemia.org http://felineleukemia.org/mailman/listinfo/felvtalk_felineleukemia.org ___ Felvtalk mailing list Felvtalk@felineleukemia.org http://felineleukemia.org/mailman/listinfo/felvtalk_felineleukemia.org -- Go Get a Life---Go Get a Shelter Animal! Ask your local animal pound to start saving over 90% of their intake by implementing the No Kill Equation: http://www.nokilladvocacycenter.org/ Legislate better animal pound conditions: http://www.rescue50.org ___ Felvtalk mailing list Felvtalk@felineleukemia.org http://felineleukemia.org/mailman/listinfo/felvtalk_felineleukemia.org
Re: [Felvtalk] Elisa/IFA clarification
This doesn't seem to deal at all with the significance of a negative IFA test after a positive Elisa. It only refers to a positive IFA test. On 6/15/2011 3:32 PM, Natalie wrote: Interesting flow chart! A good one to print and keep! I posted this recently; it also helps to understand the difference between ELISA and IFA.\ http://www.natural-cat-health-remedies.com/feline-leukemia-test.html Natalie -Original Message- From: felvtalk-boun...@felineleukemia.org [mailto:felvtalk-boun...@felineleukemia.org] On Behalf Of Sharyl Sent: Wednesday, June 15, 2011 3:53 PM To: felvtalk@felineleukemia.org Subject: Re: [Felvtalk] Elisa/IFA clarification There has been a lot of posts on this subject. This is the link to a flow chart showing what to do when you get a test result. http://www.felineleukemia.org/felvhlth.html It also states when to retest and what test to do. I've found it very helpful. Sharyl From: Barb Moermondmr_mok...@yahoo.com To: felvtalk@felineleukemia.org Sent: Wednesday, June 15, 2011 11:55 AM Subject: [Felvtalk] Elisa/IFA clarification I asked my vets for clarification on the significance of these two tests and here is their response. Here is the information from the Cornell University website about Feline Leukemia stages and testing. If you would like more information, you can go to their link athttp://www.vet.cornell.edu/fhc/brochures/felv.html I understand there are two stages of FeLV infection. What are they? FeLV is present in the blood (a condition called viremia) during two different stages of infection: · Primary viremia, an early stage of virus infection. During this stage some cats are able to mount an effective immune response, eliminate the virus from the bloodstream, and halt progression to the secondary viremia stage. · Secondary viremia, a later stage characterized by persistent infection of the bone marrow and other tissue. If FeLV infection progresses to this stage it has passed a point of no return: the overwhelming majority of cats with secondary viremia will be infected for the remainder of their lives. How is infection diagnosed? Two types of FeLV blood tests are in common use. Both detect a protein component of the virus as it circulates in the bloodstream. · ELISA (enzyme-linked immunosorbent assay) and similar tests can be performed in your veterinarian's office. ELISA-type tests detect both primary and secondary stages of viremia. · IFA (indirect immunofluorescent antibody assay) tests must be sent out to a diagnostic laboratory. IFA tests detect secondary viremia only, so the majority of positive-testing cats remain infected for life. Each testing method has strengths and weaknesses. Your veterinarian will likely suggest an ELISA-type test first, but in some cases, both tests must be performed—and perhaps repeated—to clarify a cat's true infection status. Barb+Smoky the House Puma+El Bandito Malito My cat the clown: paying no mind to whom he should impress. Merely living his life, doing what pleases him, and making me smile. - Anonymous ___ Felvtalk mailing list Felvtalk@felineleukemia.org http://felineleukemia.org/mailman/listinfo/felvtalk_felineleukemia.org ___ Felvtalk mailing list Felvtalk@felineleukemia.org http://felineleukemia.org/mailman/listinfo/felvtalk_felineleukemia.org ___ Felvtalk mailing list Felvtalk@felineleukemia.org http://felineleukemia.org/mailman/listinfo/felvtalk_felineleukemia.org ___ Felvtalk mailing list Felvtalk@felineleukemia.org http://felineleukemia.org/mailman/listinfo/felvtalk_felineleukemia.org
Re: [Felvtalk] ELISA IFA
for the Rapid Diagnosis of Feline Immunodeficiency and Feline Leukaemia Virus Infections, Vet. Rec., September, 149:317-320. 5. Herring, E. S., G. C. Troy, T. E. Toth, S. D. Forrester, L. A. Weigt, and I. P. Herring (2001), Detection of Feline Leukaemia Virus in Blood and Bone Marrow of Cats with Varying Suspicion of Latent Infection, J. Fel. Med. and Surg., 3:133-141. Kathryn Hahn on 09/24/2008 9:25:49 am ET *Posted In Message Boards 2.0 Hi there Just a quick question...when running an Idexx snap test for FeLV/FIV is there a preference for using whole blood vs serum vs plasma for the most accurate test result? Thank you! Susan Little on 09/24/2008 2:02:40 pm ET *Posted In Message Boards 2.0 Here's what the test kit insert says about samples: Serum, plasma or anticoagulated whole blood (e.g., EDTA, heparin), either fresh or stored at 2°-7°C (35°-45°F) for up to one week, can be used. For longer storage, serum or plasma can be frozen (-20°C/-4°F or colder) and then recentrifuged before use. Hemolyzed or lipemic samples will not affect results. In the past, there were concerns that using whole blood caused more inaccurate or hard to read results with the in-clinic ELISA tests, but I don't think there is current evidence that this is the case for the IDEXX Snap test. Susan Little DVM Diplomate ABVP (Feline) Ottawa, Canada [EMAIL PROTECTED] Roscoe Village Animal Hospital, P.C. 3131 North Clybourn Ave Chicago, IL 60618 773.549.3131 -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of MaryChristine Sent: Saturday, November 22, 2008 8:09 PM To: felvtalk@felineleukemia.org Subject: Re: [Felvtalk] ELISA IFA okay, well, no one really wants to hear THIS one, but yes, using serum makes the odds that an ELISA is accurate MUCH MUCH higher. in fact, as recently as last november, a famous felv researcher (intials np) stated in a private note to someone other than myself that an ELISA done on whole blood is essentially useless. gee, great, this is at the same time that other professionals' are trying to assure us that standard, done-at-the-vet's-office ELISA's (almost ALWAYS done with whole blood) are highly accurate. i just found out about this about a month ago, and still haven't digested it and what it means when the real experts (as opposed to those who just have chosen ignorance) can't agree. _ IRS CIRCULAR 230 NOTICE. Any tax advice expressed above by Mayer Brown LLP was not intended or written to be used, and cannot be used, by any taxpayer to avoid U.S. federal tax penalties. If such advice was written or used to support the promotion or marketing of the matter addressed above, then each offeree should seek advice from an independent tax advisor. This email and any files transmitted with it are intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. ___ Felvtalk mailing list Felvtalk@felineleukemia.org http://felineleukemia.org/mailman/listinfo/felvtalk_felineleukemia.org
Re: [Felvtalk] ELISA IFA
Thanks so, so much MC. Really really appreciate your feedback.VERY interesting about the serum business. I guess this will be the next stage for Daisy. I'll keep you and the list posted. Kerry From: MaryChristine [EMAIL PROTECTED] To: felvtalk@felineleukemia.org Sent: Saturday, November 22, 2008 8:09:30 PM Subject: Re: [Felvtalk] ELISA IFA okay, well, no one really wants to hear THIS one, but yes, using serum makes the odds that an ELISA is accurate MUCH MUCH higher. in fact, as recently as last november, a famous felv researcher (intials np) stated in a private note to someone other than myself that an ELISA done on whole blood is essentially useless. gee, great, this is at the same time that other professionals' are trying to assure us that standard, done-at-the-vet's-office ELISA's (almost ALWAYS done with whole blood) are highly accurate. i just found out about this about a month ago, and still haven't digested it and what it means when the real experts (as opposed to those who just have chosen ignorance) can't agree. as for the confusion over the IFA being done at the same time as an ELISA, you're right and wrong, kerry--if it's the FIRST testing, and there has been no time for the virus to have worked itself out of kitty's system, then yes, the results will often be the same, based on the same antigenic response. however, after exposure time has been factored in, the IFA is considered the confirmatory test. so if you do an ELISA and get a positive, but do a negative IFA at the same time, cat is considered negative. if you do an ELISA and get a positive and get a positive IFA at the same time, cat is considered iffy and still could be testing due to exposure. if you RETEST and get another positive IFA (most vets don't actually bother doing more than one ELISA, actually, going straight to the IFA after the first ELISA if its positive), and more than 120 days from last possible exposure has occurred, then kitty is considered positive. the whole, occult thing is something that i don't particularly think has been proven yet--using that as a theory, it means that ANY CAT THAT HAS EVER BEEN OUTDOORS OR POSSIBLY EXPOSED TO A POSITIVE CAT CAN SUDDENLY become positive--and we know that doesn't happen. look at the merck--even it says that 70% of adult healthy cats don't stay positive--i think that's a hold-over hypothesis from when it wasn't understood that a NEGATIVE test doesn' mean anything, either, really--that every cat that could have been exposed needs to be retested after 120 days, so that the true occult infection time is really the same EXPOSURE time that is involved both in incubatoin and processing the virus out of the system. the hypothesis of carriers and hidden, untestable populations made sense back when little was known, but there is too much evidence for mixed households, and feral colonies with stable FeLV populations--with little evidence that cats who suddenly test positive were ever truly negative--remember that all my cats were originally exposed by a beautiful tortie who most decidedly tested negative at the shelter before she came home--and died fully positive a little over a year later. none of the very young, nor the older, frail cats in the house ever turned positive. (anny and mysti were 3 months old when brownee moved in, they're going on nine now, so i guess they COULD be harboring it occultly..) um, what was the question again? -- Spay Neuter Your Neighbors! Maybe That'll Make The Difference MaryChristine Special-Needs Coordinator, Purebred Cat Breed Rescue (www.purebredcats.org) Member, SCAT (Special-Cat Action Team) ___ Felvtalk mailing list Felvtalk@felineleukemia.org http://felineleukemia.org/mailman/listinfo/felvtalk_felineleukemia.org ___ Felvtalk mailing list Felvtalk@felineleukemia.org http://felineleukemia.org/mailman/listinfo/felvtalk_felineleukemia.org
Re: [Felvtalk] ELISA IFA
okay, well, no one really wants to hear THIS one, but yes, using serum makes the odds that an ELISA is accurate MUCH MUCH higher. in fact, as recently as last november, a famous felv researcher (intials np) stated in a private note to someone other than myself that an ELISA done on whole blood is essentially useless. gee, great, this is at the same time that other professionals' are trying to assure us that standard, done-at-the-vet's-office ELISA's (almost ALWAYS done with whole blood) are highly accurate. i just found out about this about a month ago, and still haven't digested it and what it means when the real experts (as opposed to those who just have chosen ignorance) can't agree. as for the confusion over the IFA being done at the same time as an ELISA, you're right and wrong, kerry--if it's the FIRST testing, and there has been no time for the virus to have worked itself out of kitty's system, then yes, the results will often be the same, based on the same antigenic response. however, after exposure time has been factored in, the IFA is considered the confirmatory test. so if you do an ELISA and get a positive, but do a negative IFA at the same time, cat is considered negative. if you do an ELISA and get a positive and get a positive IFA at the same time, cat is considered iffy and still could be testing due to exposure. if you RETEST and get another positive IFA (most vets don't actually bother doing more than one ELISA, actually, going straight to the IFA after the first ELISA if its positive), and more than 120 days from last possible exposure has occurred, then kitty is considered positive. the whole, occult thing is something that i don't particularly think has been proven yet--using that as a theory, it means that ANY CAT THAT HAS EVER BEEN OUTDOORS OR POSSIBLY EXPOSED TO A POSITIVE CAT CAN SUDDENLY become positive--and we know that doesn't happen. look at the merck--even it says that 70% of adult healthy cats don't stay positive--i think that's a hold-over hypothesis from when it wasn't understood that a NEGATIVE test doesn' mean anything, either, really--that every cat that could have been exposed needs to be retested after 120 days, so that the true occult infection time is really the same EXPOSURE time that is involved both in incubatoin and processing the virus out of the system. the hypothesis of carriers and hidden, untestable populations made sense back when little was known, but there is too much evidence for mixed households, and feral colonies with stable FeLV populations--with little evidence that cats who suddenly test positive were ever truly negative--remember that all my cats were originally exposed by a beautiful tortie who most decidedly tested negative at the shelter before she came home--and died fully positive a little over a year later. none of the very young, nor the older, frail cats in the house ever turned positive. (anny and mysti were 3 months old when brownee moved in, they're going on nine now, so i guess they COULD be harboring it occultly..) um, what was the question again? -- Spay Neuter Your Neighbors! Maybe That'll Make The Difference MaryChristine Special-Needs Coordinator, Purebred Cat Breed Rescue (www.purebredcats.org) Member, SCAT (Special-Cat Action Team) ___ Felvtalk mailing list Felvtalk@felineleukemia.org http://felineleukemia.org/mailman/listinfo/felvtalk_felineleukemia.org
