[galaxy-dev] Setting genome build on datasets transferred from sample tracking external service

2012-03-30 Thread Leandro Hermida
Dear Galaxy Dev,

Is there a way to set the genome build metadata on datasets
transferred from an external service in the sample tracking system?

-Leandro
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[galaxy-dev] Questions about configuring upload via FTP

2012-03-30 Thread Sarah Diehl

Hi everybody,

how up-to-date is http://wiki.g2.bx.psu.edu/Admin/Config/Upload%20via%20FTP?

I just set up a ProFTPd according to those directions, but had to change 
some things, especially regarding permissions of directories and files. 
I had to set


Umask 000
CreateHome on 755 dirmode 755

All files and directories are created by the user nobody the server 
runs as. However, the galaxy user needs to access those and also wants 
to delete files. So I had the make the directories readable to all and 
the files even writeable for all.


Is there a better way to do this? I tried running proftpd as the galaxy 
user, but the files still belonged to nobody. All my attempts to get the 
server to create files as the galaxy user or chown to galaxy failed.


Best regards,
Sarah
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Re: [galaxy-dev] Sample tracking data transfer hangs in queue forever

2012-03-30 Thread Greg Von Kuster
Hello Emily,

This issue should be resolved in change set 6924:12b14f3e78e9, which is 
currently only available from our central repository.  It will not be available 
in the dist repository fro some time, so you'll have to pull it from Galaxy 
central (https://bitbucket.org/galaxy/galaxy-central) if you want it now.

Thanks very much for reporting this problem, and we apologize for the 
inconvenience it caused.

Greg Von Kuster


On Mar 29, 2012, at 1:49 PM, TerAvest, Emily wrote:

 
 Hi Leandro, 
 
 I am also experiencing the same problem with the latest version of galaxy. I 
 just attempted to connect our sequencer for the first time yesterday. I do 
 not have an older version of galaxy to test and compare to see if it works in 
 earlier versions. I am able to transfer data from the sequence to the import 
 directory, however it is not moved to the data library. My data_transfer.log 
 also has the same error. 
 The server could not comply with the request since it is either malformed or 
 otherwise incorrect.
 
 Does anyone have a solution for this? 
 
 Thanks
 Emily 
 
 
 
 --
 
 Message: 17
 Date: Wed, 28 Mar 2012 13:15:47 +0200
 From: Leandro Hermida soft...@leandrohermida.com
 To: Luobin Yang yangl...@isu.edu
 Cc: Galaxy Dev galaxy-...@bx.psu.edu
 Subject: Re: [galaxy-dev] Sample tracking data transfer hangs in
   queue  forever
 Message-ID:
   caohzmpj67udrqbhfchgyxnrxsm3qcy1c7jna1kr0ajbnkek...@mail.gmail.com
 Content-Type: text/plain; charset=ISO-8859-1
 
 Dear Galaxy Dev,
 
 Ok I have gotten further found out what was going on, in my
 data_transfer.log I was getting HTTP 404 Not Authorized when the data
 transfer was trying to access Galaxy API URLs
 http://galaxyserver/api/... This is because we are using external user
 authentication as documented in
 http://wiki.g2.bx.psu.edu/Admin/Config/Apache%20Proxy and this puts
 all of Galaxy behind this authentication. Since the Galaxy API uses
 API keys to essentially authenticate you have to change you Location
 / ... /Location container to not match URLs starting with /api. To
 do this you have to change it to LocationMatch ^/(?!api) 
 /LocationMatch.
 
 best,
 leandro
 
 On Mon, Mar 26, 2012 at 6:10 PM, Luobin Yang yangl...@isu.edu wrote:
 I've got the same problem and when I looked at the data_transfer.log, I saw
 the following message:
 
 2012-03-01 15:12:27,338 - datatx_13870 -
 (u'9c17d84742cd2acb63d88b5bd41d968f',
 u'http://xxx.xxx.xxx.xxx/api/samples/2d9035b3fc152403',
 {'sample_dataset_ids': ['a799d38679e985db', '33b43b4e7093c91f'],
 'error_msg': '', 'update_type': 'sample_dataset_transfer_status',
 'new_status': 'Adding to data library'})
 2012-03-01 15:12:27,342 - datatx_13870 - Error. !DOCTYPE HTML PUBLIC
 -//IETF//DTD HTML 2.0//EN
 htmlhead
 title405 Method Not Allowed/title
 /headbody
 h1Method Not Allowed/h1
 pThe requested method PUT is not allowed for the URL
 /api/samples/2d9035b3fc152403./p
 hr
 
 Using Galaxy instead of Apache as the web server changed the sample status
 from in queue to complete but didn't add the downloaded files to the data
 library.
 
 Luobin
 
 On Mon, Mar 26, 2012 at 9:54 AM, Leandro Hermida
 soft...@leandrohermida.com wrote:
 
 Dear Galaxy Dev,
 
 I've set up the Galaxy sample tracking system data transfer
 functionality exactly as specified here
 https://main.g2.bx.psu.edu/u/rkchak/p/data-transfer, but when I
 attempt to transfer datasets it puts them into the queue and then
 never seems to transfer anything.
 
 The galaxy_listener.log shows:
 
 2012-03-26 17:16:24,515 - GalaxyAMQP - GALAXY LISTENER PID: 8738 -
 {'config_file': 'universe_wsgi.ini', 'http_server_section':
 'server:main'}
 2012-03-26 17:16:24,518 - GalaxyAMQP - {'exchange': 'galaxy_exchange',
 'rabbitmqctl_path':
 
 '/gpfshpc/software/pmrdcdb/galaxy/rabbitmq/rabbitmq_server-2.8.1/sbin/rabbitmqctl',
 'userid': 'galaxy_dev', 'routing_key': 'bar_code_scanner', 'queue':
 'galaxy_queue', 'host': '127.0.0.1', 'virtual_host':
 'galaxy_dev_messaging_engine', 'password': 'mypassword', 'port':
 '5672'}
 2012-03-26 17:16:24,565 - GalaxyAMQP - Connected to rabbitmq server -
 127.0.0.1:5672
 2012-03-26 17:31:23,393 - GalaxyAMQP - MESSAGE RECVD: data_transfer
 2012-03-26 17:31:23,393 - GalaxyAMQP - DATA TRANSFER
 2012-03-26 17:31:23,401 - GalaxyAMQP - Started process (25406): python
 
 /gpfshpc/software/pmrdcdb/galaxy/galaxy_dist_dev/scripts/galaxy_messaging/server/data_transfer.py
  data_transfer
 galaxy_hostrd-galaxydev.app.pmi/galaxy_host
 api_key69bbd1a9b18eda2a888e9963820d8ef0/api_key
 data_hostchp723/data_host
 data_usergalaxy/data_user
 data_passwordmypassword/data_password
 request_id12/request_id
 sample_id12/sample_id
 library_id15/library_id
 folder_id52/folder_id ? ? ? ? ? ? ? ? ? ?dataset
 ?dataset_id10/dataset_id
 namettt_2.fastq.gz/name
 file/gpfshpc/data/pmrdcdb/RNA-Seq/datasets/ttt-2.fastq.gz/file
 ? ? ? ? ?/datasetdataset
 dataset_id9/dataset_id
 namettt_1.fastq.gz/name
 

Re: [galaxy-dev] [galaxy-user] CummeRband in Galaxy platform

2012-03-30 Thread Carlos Borroto
Hi Bomba,

I'm also quite interested on getting cummeRbund into Galaxy. I was
able to start developing a tool to use it, but with tough time
constraints at work and school, I haven't been able to get it to a
point where I'm happy publishing it in the Main Toolshed. It is
however in the Test Toolshed[1], you could find it by searching. If
you have a local or cloud instance you should be able to install it
and start playing with it. I got to the point where you can generate
most kind of plots and you can select what kind of feature you want to
plot, genes/isoform/TSS/CDS.

There is a lot to do still. For example I think it is a most to be
able to use a list of genes as input for some of the graphs, like
scatterplots and heatmaps, right now you can do this but you have to
enter the genes one by one. There is no support for any non-plotting
function, like getSig() or getSigTable(). Hopefully I will find the
time in the near future to get it in shape for submission to the Main
Toolshed. This however doesn't mean it would appear in the Main Galaxy
sever, that's a decision only Galaxy developers can make.

Lastly, if there is interest in contributing to this effort, please
let me know as I'll be happy to collaborate with other interested
party developing this tool.

[1]http://testtoolshed.g2.bx.psu.edu/

Regards,
Carlos

On Fri, Mar 30, 2012 at 9:45 AM, Bomba Dam
bomba@visva-bharati.ac.in wrote:
 Hi Galaxy team,

 Are there any plans to add the CummeRband tool in the Galaxy platform.

 It would be very nice to have it within Galaxy, this will help people like
 us (Biologists, without much programming knowledge) to do the complete
 RNA-seq analysis and visualize the data in graphical forms

 Cheers.

 Bomba

 --





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