[galaxy-dev] GATK - Depth of Coverage Error

2013-08-15 Thread Can Holyavkin
Hi Everyone,

Recently, I tried to use GATK - Depth of Coverage tool with my own .bam file on 
Galaxy for the first time. However it gave following error for each output. 
(see screenshot)

 Depth of Coverage on data 4 and data 1 (output cumulative coverage 
 proportions sample)
 error:
 An error occurred with this dataset: /bin/sh: 1: samtools: not found 
 Traceback (most recent call last): File 
 /home/can/galaxy-dist/tools/gatk/gatk_wrapper.py, line 126, in if 
 __name__==__main__: __main__() File 
 /home/can/galaxy-dist/tools/gatk/gatk_wrapper.py, line 9


I searched the mailing list, but I couldn't find similar problem that posted 
before.

How can I solve it? Should I install GATK seperately on Galaxy? or anything 
else?

Can Holyavkin
PhD Student
Istanbul Technical University
Molecular Biology and Genetics Dept.___
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Re: [galaxy-dev] One small problem for experts, one giant question for me..

2013-08-12 Thread Can Holyavkin
Dear Bjoern,

I was using ubuntu with Turkish language. Your answer gave me clue about the 
problem. Turkish language contains some special characters that may confuse 
Galaxy. So, I turned it into English. Voila! Problem solved. :)

Thank you so much Bjoern. :) You are the best!

Can Holyavkin
___
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[galaxy-dev] One small problem for experts, one giant question for me..

2013-08-09 Thread Can Holyavkin
Hello everyone,

Firstly, I am beginner. I am a new Galaxy user that installed the Galaxy 
locally into my ubuntu (13.04) installed computer. Everything is working 
perfectly right now. Galaxy is really awesome.

To learn the basics, I started the basic tutorial (Galaxy101 
https://main.g2.bx.psu.edu/u/aun1/p/galaxy101) that helps too much. This 
tutorial consist a step for explaning Compare Two Datasets function. I did 
exactly everthing the instructor said however that module become red in History 
with following error:

tool error
An error occured with dataset: 
Unable to finish job (screenshot of error: http://i.imgur.com/xHqhK72.png)

I wrote the bug report at end of the mail.

Interestingly, when I press View button, I get the data that I wanted. 
However, any metadata is seen. So I can not see the final results on the UCSC 
browser. I replicate the same module at public server, it didn't gave an error. 

What might cause this kind of problem? Does reinstalling solve this problem? 
Would you please help me?

Thank you everyone. 

Traceback (most recent call last):
  File /home/can/galaxy-dist/lib/galaxy/jobs/runners/local.py, line 94, in 
queue_job
job_wrapper.finish( stdout, stderr, exit_code )
  File /home/can/galaxy-dist/lib/galaxy/jobs/__init__.py, line 1009, in finish
self.sa_session.flush()
  File 
/home/can/galaxy-dist/eggs/SQLAlchemy-0.7.9-py2.7-linux-x86_64-ucs4.egg/sqlalchemy/orm/scoping.py,
 line 114, in do
return getattr(self.registry(), name)(*args, **kwargs)
  File 
/home/can/galaxy-dist/eggs/SQLAlchemy-0.7.9-py2.7-linux-x86_64-ucs4.egg/sqlalchemy/orm/session.py,
 line 1718, in flush
self._flush(objects)
  File 
/home/can/galaxy-dist/eggs/SQLAlchemy-0.7.9-py2.7-linux-x86_64-ucs4.egg/sqlalchemy/orm/session.py,
 line 1789, in _flush
flush_context.execute()
  File 
/home/can/galaxy-dist/eggs/SQLAlchemy-0.7.9-py2.7-linux-x86_64-ucs4.egg/sqlalchemy/orm/unitofwork.py,
 line 331, in execute
rec.execute(self)
  File 
/home/can/galaxy-dist/eggs/SQLAlchemy-0.7.9-py2.7-linux-x86_64-ucs4.egg/sqlalchemy/orm/unitofwork.py,
 line 475, in execute
uow
  File 
/home/can/galaxy-dist/eggs/SQLAlchemy-0.7.9-py2.7-linux-x86_64-ucs4.egg/sqlalchemy/orm/persistence.py,
 line 59, in save_obj
mapper, table, update)
  File 
/home/can/galaxy-dist/eggs/SQLAlchemy-0.7.9-py2.7-linux-x86_64-ucs4.egg/sqlalchemy/orm/persistence.py,
 line 485, in _emit_update_statements
execute(statement, params)
  File 
/home/can/galaxy-dist/eggs/SQLAlchemy-0.7.9-py2.7-linux-x86_64-ucs4.egg/sqlalchemy/engine/base.py,
 line 1449, in execute
params)
  File 
/home/can/galaxy-dist/eggs/SQLAlchemy-0.7.9-py2.7-linux-x86_64-ucs4.egg/sqlalchemy/engine/base.py,
 line 1584, in _execute_clauseelement
compiled_sql, distilled_params
  File 
/home/can/galaxy-dist/eggs/SQLAlchemy-0.7.9-py2.7-linux-x86_64-ucs4.egg/sqlalchemy/engine/base.py,
 line 1698, in _execute_context
context)
  File 
/home/can/galaxy-dist/eggs/SQLAlchemy-0.7.9-py2.7-linux-x86_64-ucs4.egg/sqlalchemy/engine/base.py,
 line 1691, in _execute_context
context)
  File 
/home/can/galaxy-dist/eggs/SQLAlchemy-0.7.9-py2.7-linux-x86_64-ucs4.egg/sqlalchemy/engine/default.py,
 line 331, in do_execute
cursor.execute(statement, parameters)
ProgrammingError: (ProgrammingError) You must not use 8-bit bytestrings unless 
you use a text_factory that can interpret 8-bit bytestrings (like text_factory 
= str). It is highly recommended that you instead just switch your application 
to Unicode strings. u'UPDATE job SET update_time=?, stdout=?, stderr=? WHERE 
job.id = ?' ('2013-08-09 09:00:48.464260', 'join (GNU coreutils) 
8.20\nCopyright (C) 2012 Free Software Foundation, Inc.\nLisans GPLv3+: GNU GPL 
s\xc3\xbcr\xc3\xbcm 3 ve sonras\xc4\xb1 http://gnu.org/licenses/gpl.html.\nBu 
\xc3\x96zg\xc3\xbcr bir yaz\xc4\xb1l\xc4\xb1md\xc4\xb1r: 
de\xc4\x9fi\xc5\x9ftirmek ve da\xc4\x9f\xc4\xb1tmakta 
\xc3\xb6zg\xc3\xbcrs\xc3\xbcn\xc3\xbcz.\nYasalar\xc4\xb1n izin verdi\xc4\x9fi 
\xc3\xb6l\xc3\xa7\xc3\xbcde, herhangi bir GARANT\xc4\xb0 sunulmaz.\n\nMike 
Haertel taraf\xc4\xb1ndan yaz\xc4\xb1ld\xc4\xb1.\n', '', 41)


Can Holyavkin

PhD Student
Istanbul Technical University
Molecular Biology and Genetics Dept.___
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