[galaxy-user] How to define the cutoff value of RPKM for expressed genes?

2013-07-03 Thread Hoang, Thanh
Hi all,
I have been working on RNA-seq data analysis using TopHat and Cuffdiff. One
of the problem I have is to define the cutoff RPKM value to tell whether a
gene is expressed from  the background noise?.
 Could anybody give me a suggestion?
Thank you
Thanh
___
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org.  Please keep all replies on the list by
using reply all in your mail client.  For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:

  http://lists.bx.psu.edu/listinfo/galaxy-dev

To manage your subscriptions to this and other Galaxy lists,
please use the interface at:

  http://lists.bx.psu.edu/

To search Galaxy mailing lists use the unified search at:

  http://galaxyproject.org/search/mailinglists/

Re: [galaxy-user] How to define the cutoff value of RPKM for expressed genes?

2013-07-03 Thread Jeremy Goecks
The confidence intervals provided by Cufflinks/Cuffdiff are a good place to 
start; any confidence interval that includes 0 should be looked on skeptically.

Good luck,
J.

On Jul 3, 2013, at 2:52 PM, Hoang, Thanh wrote:

 Hi all,
 I have been working on RNA-seq data analysis using TopHat and Cuffdiff. One 
 of the problem I have is to define the cutoff RPKM value to tell whether a 
 gene is expressed from  the background noise?.
  Could anybody give me a suggestion?
 Thank you
 Thanh
 ___
 The Galaxy User list should be used for the discussion of
 Galaxy analysis and other features on the public server
 at usegalaxy.org.  Please keep all replies on the list by
 using reply all in your mail client.  For discussion of
 local Galaxy instances and the Galaxy source code, please
 use the Galaxy Development list:
 
  http://lists.bx.psu.edu/listinfo/galaxy-dev
 
 To manage your subscriptions to this and other Galaxy lists,
 please use the interface at:
 
  http://lists.bx.psu.edu/
 
 To search Galaxy mailing lists use the unified search at:
 
  http://galaxyproject.org/search/mailinglists/


___
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org.  Please keep all replies on the list by
using reply all in your mail client.  For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:

  http://lists.bx.psu.edu/listinfo/galaxy-dev

To manage your subscriptions to this and other Galaxy lists,
please use the interface at:

  http://lists.bx.psu.edu/

To search Galaxy mailing lists use the unified search at:

  http://galaxyproject.org/search/mailinglists/