[Histonet] Microtome calibration
Dear all Is anyone practising microtome calibration for thickness of sections cut? Our GLP auditors are frequently asking for it. They also suggest that automatic tissue processor time in each reagent should be calibrated. any comments Regards Dr Girish India Disclaimer This message contains legally privileged and/or confidential information. If you are not the intended recipient(s), or employee or agent responsible for delivery of this message to the intended recipient(s), you are hereby notified that any dissemination, distribution or copying of this e-mail message is strictly prohibited. If you have received this message in error, please immediately notify the sender and delete this e-mail message from your computer. WARNING: Computer viruses can be transmitted via email. The recipient should check this email and any attachments for the presence of viruses. The company accepts no liability for any damage caused by any virus transmitted by this email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PIN-4 controls
Happy Halloween! Does anyone know of a good PIN-4 control? We are currently using in-house tissue but are finding a lot of variance with the stainability. If anyone knows of a good source I would greatly appreciate it. Thanks in advance, Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 (704) 549-8444 x104 [EMAIL PROTECTED] ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] pronase vs. proteinase-k
Are they the same ? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] anti-mouse ferritin
Hi, Any recommedations for anti-mouse ferritin antibodies (looking to stain FFPE mouse tissue). Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtome calibration
Dear Dr. Girish, I agree with rene, if the qualitiy of the cuts is fine it doesn't matter if they are 1µm or 2.3µm thick. But I'm highly interested in how your GLP auditors want to calibrate the thickness of the section cuts. Are you supposed to measure the slides after cutting?! Before or after stretching in the water bath?! If they have a protocol how to do this I would be happy if they can share it with us. Frauke Quoting Rene J Buesa [EMAIL PROTECTED]: Dr. Girish: BOTH requirements are rubbish invented by bureaucrats with lots of time in their hands and trying to appear concerned and knowledgeable. Thickness is not necessary as long as the section is diagnostically useful or if some quantitative method as to the intensity is done in which case thickness = amount of matter, and would influence the outcome of the quantitative process. Timing the tissue processor is also totally irrelevant because it does not really matter a few minutes each way during a processing protocol. More important would be to keep a record of the fixation time that is the only step really critical in tissue processing. René J. --- On Fri, 10/31/08, [EMAIL PROTECTED] [EMAIL PROTECTED] wrote: From: [EMAIL PROTECTED] [EMAIL PROTECTED] Subject: [Histonet] Microtome calibration To: histonet@lists.utsouthwestern.edu Date: Friday, October 31, 2008, 2:49 AM Dear all Is anyone practising microtome calibration for thickness of sections cut? Our GLP auditors are frequently asking for it. They also suggest that automatic tissue processor time in each reagent should be calibrated. any comments Regards Dr Girish India ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message was sent using IMP, the Internet Messaging Program. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtome calibration
Frauke: That calibration is ussually done by measuring the advance mechanism of the block holder and how many µm the block moves towards the blade, but that is rubbish. René J. --- On Fri, 10/31/08, Dr. med. Frauke Neff [EMAIL PROTECTED] wrote: From: Dr. med. Frauke Neff [EMAIL PROTECTED] Subject: Re: [Histonet] Microtome calibration To: [EMAIL PROTECTED] Cc: histonet@lists.utsouthwestern.edu, [EMAIL PROTECTED] Date: Friday, October 31, 2008, 9:41 AM Dear Dr. Girish, I agree with rene, if the qualitiy of the cuts is fine it doesn't matter if they are 1µm or 2.3µm thick. But I'm highly interested in how your GLP auditors want to calibrate the thickness of the section cuts. Are you supposed to measure the slides after cutting?! Before or after stretching in the water bath?! If they have a protocol how to do this I would be happy if they can share it with us. Frauke Quoting Rene J Buesa [EMAIL PROTECTED]: Dr. Girish: BOTH requirements are rubbish invented by bureaucrats with lots of time in their hands and trying to appear concerned and knowledgeable. Thickness is not necessary as long as the section is diagnostically useful or if some quantitative method as to the intensity is done in which case thickness = amount of matter, and would influence the outcome of the quantitative process. Timing the tissue processor is also totally irrelevant because it does not really matter a few minutes each way during a processing protocol. More important would be to keep a record of the fixation time that is the only step really critical in tissue processing. René J. --- On Fri, 10/31/08, [EMAIL PROTECTED] [EMAIL PROTECTED] wrote: From: [EMAIL PROTECTED] [EMAIL PROTECTED] Subject: [Histonet] Microtome calibration To: histonet@lists.utsouthwestern.edu Date: Friday, October 31, 2008, 2:49 AM Dear all Is anyone practising microtome calibration for thickness of sections cut? Our GLP auditors are frequently asking for it. They also suggest that automatic tissue processor time in each reagent should be calibrated. any comments Regards Dr Girish India ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message was sent using IMP, the Internet Messaging Program. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Microtome calibration
We have a GLP person who is not familiar with Histology at all. We have had to educate her about these issues and may other things. Calibration finally came down to having a PM on the units every year. After she understood all the things we do with a section during the cutting phase and pick up that would alter a true measurement. We also do MMA sections on the microtome and even those are next to impossible to measure due to some stretching of the section during pick up and drying. Oh Yes, the drying of the slides really put her in a tail spin as this was just not allowing the sections to stay the same as when they were picked up. Sometimes a long talk and demonstration is the only way to make the point that we are not standardized like clinical chemistry etc. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Rene J Buesa Sent: Friday, October 31, 2008 10:08 AM To: Dr. med. Frauke Neff Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome calibration Frauke: That calibration is ussually done by measuring the advance mechanism of the block holder and how many µm the block moves towards the blade, but that is rubbish. René J. --- On Fri, 10/31/08, Dr. med. Frauke Neff [EMAIL PROTECTED] wrote: From: Dr. med. Frauke Neff [EMAIL PROTECTED] Subject: Re: [Histonet] Microtome calibration To: [EMAIL PROTECTED] Cc: histonet@lists.utsouthwestern.edu, [EMAIL PROTECTED] Date: Friday, October 31, 2008, 9:41 AM Dear Dr. Girish, I agree with rene, if the qualitiy of the cuts is fine it doesn't matter if they are 1µm or 2.3µm thick. But I'm highly interested in how your GLP auditors want to calibrate the thickness of the section cuts. Are you supposed to measure the slides after cutting?! Before or after stretching in the water bath?! If they have a protocol how to do this I would be happy if they can share it with us. Frauke Quoting Rene J Buesa [EMAIL PROTECTED]: Dr. Girish: BOTH requirements are rubbish invented by bureaucrats with lots of time in their hands and trying to appear concerned and knowledgeable. Thickness is not necessary as long as the section is diagnostically useful or if some quantitative method as to the intensity is done in which case thickness = amount of matter, and would influence the outcome of the quantitative process. Timing the tissue processor is also totally irrelevant because it does not really matter a few minutes each way during a processing protocol. More important would be to keep a record of the fixation time that is the only step really critical in tissue processing. René J. --- On Fri, 10/31/08, [EMAIL PROTECTED] [EMAIL PROTECTED] wrote: From: [EMAIL PROTECTED] [EMAIL PROTECTED] Subject: [Histonet] Microtome calibration To: histonet@lists.utsouthwestern.edu Date: Friday, October 31, 2008, 2:49 AM Dear all Is anyone practising microtome calibration for thickness of sections cut? Our GLP auditors are frequently asking for it. They also suggest that automatic tissue processor time in each reagent should be calibrated. any comments Regards Dr Girish India ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message was sent using IMP, the Internet Messaging Program. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Polyclonal Proteolipid Protein Ab?
I have been (unsuccessfully) trying to find a polyclonal antibody against human proteolipid protein (stains myelin) that will work with immunofluorescence on FFPE sections. I have also tried some myelin basic protein with no luck. Has anyone found any in the past that have worked?? Thanks! Nicole J. Patten Post-Baccalaureate Fellow/IRTA NIAAA/National Institutes of Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formalin and Xylene Monitoring Badges
Happy Halloween... I'm looking for a source of monitoring badges for formalin and xylene, both the 8 hour TWA and the STEL. Thank you, Sandy Sandra Cheasty Histology Supervisor UW-Madison School of Veterinary Medicine 608 263-1680 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Formalin and Xylene Monitoring Badges
ASSAY TECHNOLOGY 1252 Quarry Lane Pleasanton, CA 94566 1-800-833-1258 Sandra Cheasty [EMAIL PROTECTED] 10/31/2008 08:51 Happy Halloween... I'm looking for a source of monitoring badges for formalin and xylene, both the 8 hour TWA and the STEL. Thank you, Sandy Sandra Cheasty Histology Supervisor UW-Madison School of Veterinary Medicine 608 263-1680 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Formalin and Xylene Monitoring Badges
Try Mercedes Medical. http://www.mercedesmedical.com/ Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: [EMAIL PROTECTED] -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Sandra Cheasty Sent: Friday, October 31, 2008 11:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin and Xylene Monitoring Badges Happy Halloween... I'm looking for a source of monitoring badges for formalin and xylene, both the 8 hour TWA and the STEL. Thank you, Sandy Sandra Cheasty Histology Supervisor UW-Madison School of Veterinary Medicine 608 263-1680 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formalin and Xylene Monitoring Badges
Morphix Technologies www.morphtec.com 757-431-2260 -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Kathleen Boozer Sent: Friday, October 31, 2008 11:22 AM To: histonet@lists.utsouthwestern.edu; Sandra Cheasty Subject: Re: [Histonet] Formalin and Xylene Monitoring Badges ASSAY TECHNOLOGY 1252 Quarry Lane Pleasanton, CA 94566 1-800-833-1258 Sandra Cheasty [EMAIL PROTECTED] 10/31/2008 08:51 Happy Halloween... I'm looking for a source of monitoring badges for formalin and xylene, both the 8 hour TWA and the STEL. Thank you, Sandy Sandra Cheasty Histology Supervisor UW-Madison School of Veterinary Medicine 608 263-1680 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: bone marrow biopsies
Cindi Robinson replied to me offline, saying some of the things Vinnie della Speranza also said. I wasn't aware that hydrochloric acid decalcification (I assume that regular brand-name Decal is HCl) with prompt timing degraded IHC - something I guess that every lab has to determine for themselves. Is formic acid the decalcifier of choice for marrow cores? (One more reason to be using properly identified chemicals, and not secret proprietary mixtures, as John Kiernan has so often stressed on this list.) Repeating what I said before, I think that communication between histotechnologists and pathologists, and between pathologists and oncologists, is essential here. How often is overnight turnaround critical to patient care? Can the oncologists do biopsies earlier in the day when they need overnight turnaround? Actually, this isn't the worst communication problem I've seen among these groups of people. Even more serious is getting co-operation among all parties in getting marrow smears done right. In many services I've worked in, it's almost unheard of to get a marrow specimen with properly prepared and adequately stained smears. The procedure requires constant attention to detail, and frequent training of new workers by experienced technologists and - dare I say it? - pathologists. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
