[Histonet] Re: bone marrow biopsies

2008-10-31 Thread Robert Richmond
Cindi Robinson replied to me offline, saying some of the things Vinnie
della Speranza also said. I wasn't aware that hydrochloric acid
decalcification (I assume that regular brand-name Decal is HCl) with
prompt timing degraded IHC - something I guess that every lab has to
determine for themselves. Is formic acid the decalcifier of choice for
marrow cores? (One more reason to be using properly identified
chemicals, and not secret proprietary mixtures, as John Kiernan has so
often stressed on this list.)

Repeating what I said before, I think that communication between
histotechnologists and pathologists, and between pathologists and
oncologists, is essential here. How often is overnight turnaround
critical to patient care? Can the oncologists do biopsies earlier in
the day when they need overnight turnaround?

Actually, this isn't the worst communication problem I've seen among
these groups of people. Even more serious is getting co-operation
among all parties in getting marrow smears done right. In many
services I've worked in, it's almost unheard of to get a marrow
specimen with properly prepared and adequately stained smears. The
procedure requires constant attention to detail, and frequent training
of new workers by experienced technologists and - dare I say it? -
pathologists.

Bob Richmond
Samurai Pathologist
Knoxville TN

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[Histonet] mouse heart endothelium

2008-10-31 Thread Andrea Hooper
Is anyone routinely staining for mouse heart ECs in FFPE sections? If 
so, what markers and protocol are you using?


Thanks,
ANDREA
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Re: [Histonet] Formalin and Xylene Monitoring Badges

2008-10-31 Thread Rene J Buesa
Try a company named KEM
René J.

--- On Fri, 10/31/08, Sandra Cheasty <[EMAIL PROTECTED]> wrote:

From: Sandra Cheasty <[EMAIL PROTECTED]>
Subject: [Histonet] Formalin and Xylene Monitoring Badges
To: "histonet@lists.utsouthwestern.edu" 
Date: Friday, October 31, 2008, 11:51 AM

Happy Halloween...  I'm looking for a source of monitoring badges for
formalin and xylene, both the 8 hour TWA and the STEL.

Thank you, Sandy



Sandra Cheasty

Histology Supervisor

UW-Madison

School of Veterinary Medicine

608 263-1680

















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Re: [Histonet] Re: Histonet Digest, Vol 59, Issue 40

2008-10-31 Thread KELLY BOYD
QA/QC is a real pain at times, but it is priority in my lab. I don't know any 
Histotech who likes all of the documentation! I just recently had to have one 
of our five microtomes re-calibrated because it was producing slides that were 
staining much lighter than from the other microtomes. (They are all set at 4 
micrometers) This particular microtome was also used for cutting all of our 
specials. It should be mandatory to have PMs (that include calibration) done on 
microtomes. How would an un-experienced tech know they were not cutting their 
specials at a certain thickness?
I'll do all that extra "rubbish" anytime to make sure the patients are getting 
the best care they deserve.
 
As far as fixation being the only critical part in processing, That is 
"rubbish"!!! All steps are equally important. Maybe not to the exact 
minutes though.
 
 
Happy Halloween to all!  


Kelly D. Boyd, BS, HTL (ASCP)
Lab Manager
Harris Histology Services
2025 Eastgate Dr. Ste. F
Greenville, NC 27858
www.harrishisto.com 
 
Tele (252)-830-6866
Cell  (252)-943-9527
Fax  (252)-830-0032
 
 

--- On Fri, 10/31/08, Randall Carpenter <[EMAIL PROTECTED]> wrote:

From: Randall Carpenter <[EMAIL PROTECTED]>
Subject: [Histonet] Re: Histonet Digest, Vol 59, Issue 40
To: histonet@lists.utsouthwestern.edu
Date: Friday, October 31, 2008, 1:53 PM

Dr. Girish'

I must respectfully disagree with those that consider calibrations of your
microtome and stainer times "rubbish".  I don't understand the
reluctance of many of my histology colleagues to GLP compliance and to Quality
Assurance.  Firstly without QA there is no such thing as GLP compliance.  I
understand that sometimes QA people do not know much about histotechnique. At
the same time they do know quite a bit about documentation and compliance.  Most
QA people are willing to listen, but again that should be a two way street.
In the example of the microtome, Pamela's solution is perfect.  If the
person doing the PM uses calibrated and and traceable equipment and provides you
with documentation, you're set.  What you have shown QA and the FDA (when
they show up) is that your microtome operates according to the
manufacturer's specifications.  That's it. We all know that the
thickness of a section can vary (thick-thin), but a GLP based calibration only
addresses the operating condition of your equipment.  A "trained"
histotechnician would be able to spot any problems in sectioning thickness. 
Yes?

As far as stainer times are concerned, use a calibrated and traceable timer to
check that the times match.  Do it once, document it and you're done.  Have
QA check off on it and everybody is happy.

I would suggest any lab that does a fair amount of GLP work do an IQ, OQ, PQ on
their system.  I know it can be a pain, but more and more CLIENTS are asking for
it, not just QA.  

I understand that compliance can be more work.  It is more work.  What I
don't understand is the reluctance of some to avoid making sure that their
lab is in compliance with FDA or CAP.  If your loved one had a biopsy come
through a lab, how would you feel about someone using outdated reagents on that
sample or using poorly maintained equipment?  

End communication.

Randy Carpenter
Twin Cities Histology
>
>Message: 18
>Date: Fri, 31 Oct 2008 10:20:36 -0400
>From: "Pamela Marcum" <[EMAIL PROTECTED]>
>Subject: RE: [Histonet] Microtome calibration
>To: <[EMAIL PROTECTED]>, "'Dr. med. Frauke Neff'"
>   <[EMAIL PROTECTED]>
>Cc: histonet@lists.utsouthwestern.edu
>Message-ID: <[EMAIL PROTECTED]>
>Content-Type: text/plain;  charset="iso-8859-1"
>
>We have a GLP person who is not familiar with Histology at all.  We have
had
>to educate her about these issues and may other things.  Calibration
finally
>came down to having a PM on the units every year.  After she understood all
>the things we do with a section during the cutting phase and pick up that
>would alter a true measurement. 
>
>We also do MMA sections on the microtome and even those are next to
>impossible to measure due to some stretching of the section during pick up
>and drying.  Oh Yes, the drying of the slides really put her in a tail spin
>as this was just not allowing the sections to stay the same as when they
>were picked up.  
>
>Sometimes a long talk and demonstration is the only way to make the point
>that we are not standardized like clinical chemistry etc.  
>
>Pamela A Marcum
>University of Pennsylvania 
>School of Veterinary Medicine
>Comparative Orthopedic Laboratory (CORL)
>382 W Street Rd
>Kennett Square PA 19438
>610-925-6278
>
>-Original Message-
>From: [EMAIL PROTECTED]
>[mailto:[EMAIL PROTECTED] On Behalf Of Rene J
Buesa
>Sent: Friday, October 31, 2008 10:08 AM
>To: Dr. med. Frauke Neff
>Cc: histonet@lists.utsouthwestern.edu
>Subject: Re: [Histonet] Microtome calibration
>
>Frauke:
>That calibration is ussually done by measuring the advance mechanism of the
>block holder and how many µm the block moves towards the blade, but that

[Histonet] (no subject)

2008-10-31 Thread Troutman, Kenneth A
Try the following decal from Surgipath.  We used it with great success when I 
was in another lab.  It is a 2 step decal procedure and it can significantly 
cut down on the total time.
 
http://www.surgipath.com/region/us/product.aspx?p=77
 
Ashley Troutman BS, HT(ASCP)QIHC
Histopathology Laboratory
Department of Pathology
Vanderbilt University Medical Center
Nashville, TN
  
   
 
-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Cynthia Robinson
Sent: Thursday, October 30, 2008 11:44 AM
To: histonet
Subject: [Histonet] bone marrow biopsies

We are currently using 10% formalin fixation on our bone marrow cores.  We fix 
for 2 hours minimum prior to decal.  We are using Immunocal from Decal Corp. 
for 2-4 hrs followed with processing overnight in VIP.  Cores are still crunchy 
upon sectioning and we are doing surface decal for up to 30 min. Our paths want 
cores turned out within 24 hrs following procedure. Any suggestions?

Thanks.
Cindi

  
   
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[Histonet] Re: Histonet Digest, Vol 59, Issue 40

2008-10-31 Thread Randall Carpenter
Dr. Girish'

I must respectfully disagree with those that consider calibrations of your 
microtome and stainer times "rubbish".  I don't understand the reluctance of 
many of my histology colleagues to GLP compliance and to Quality Assurance.  
Firstly without QA there is no such thing as GLP compliance.  I understand that 
sometimes QA people do not know much about histotechnique. At the same time 
they do know quite a bit about documentation and compliance.  Most QA people 
are willing to listen, but again that should be a two way street.
In the example of the microtome, Pamela's solution is perfect.  If the person 
doing the PM uses calibrated and and traceable equipment and provides you with 
documentation, you're set.  What you have shown QA and the FDA (when they show 
up) is that your microtome operates according to the manufacturer's 
specifications.  That's it. We all know that the thickness of a section can 
vary (thick-thin), but a GLP based calibration only addresses the operating 
condition of your equipment.  A "trained" histotechnician would be able to spot 
any problems in sectioning thickness.  Yes?

As far as stainer times are concerned, use a calibrated and traceable timer to 
check that the times match.  Do it once, document it and you're done.  Have QA 
check off on it and everybody is happy.

I would suggest any lab that does a fair amount of GLP work do an IQ, OQ, PQ on 
their system.  I know it can be a pain, but more and more CLIENTS are asking 
for it, not just QA.  

I understand that compliance can be more work.  It is more work.  What I don't 
understand is the reluctance of some to avoid making sure that their lab is in 
compliance with FDA or CAP.  If your loved one had a biopsy come through a lab, 
how would you feel about someone using outdated reagents on that sample or 
using poorly maintained equipment?  

End communication.

Randy Carpenter
Twin Cities Histology
>
>Message: 18
>Date: Fri, 31 Oct 2008 10:20:36 -0400
>From: "Pamela Marcum" <[EMAIL PROTECTED]>
>Subject: RE: [Histonet] Microtome calibration
>To: <[EMAIL PROTECTED]>, "'Dr. med. Frauke Neff'"
>   <[EMAIL PROTECTED]>
>Cc: histonet@lists.utsouthwestern.edu
>Message-ID: <[EMAIL PROTECTED]>
>Content-Type: text/plain;  charset="iso-8859-1"
>
>We have a GLP person who is not familiar with Histology at all.  We have had
>to educate her about these issues and may other things.  Calibration finally
>came down to having a PM on the units every year.  After she understood all
>the things we do with a section during the cutting phase and pick up that
>would alter a true measurement. 
>
>We also do MMA sections on the microtome and even those are next to
>impossible to measure due to some stretching of the section during pick up
>and drying.  Oh Yes, the drying of the slides really put her in a tail spin
>as this was just not allowing the sections to stay the same as when they
>were picked up.  
>
>Sometimes a long talk and demonstration is the only way to make the point
>that we are not standardized like clinical chemistry etc.  
>
>Pamela A Marcum
>University of Pennsylvania 
>School of Veterinary Medicine
>Comparative Orthopedic Laboratory (CORL)
>382 W Street Rd
>Kennett Square PA 19438
>610-925-6278
>
>-Original Message-
>From: [EMAIL PROTECTED]
>[mailto:[EMAIL PROTECTED] On Behalf Of Rene J Buesa
>Sent: Friday, October 31, 2008 10:08 AM
>To: Dr. med. Frauke Neff
>Cc: histonet@lists.utsouthwestern.edu
>Subject: Re: [Histonet] Microtome calibration
>
>Frauke:
>That calibration is ussually done by measuring the advance mechanism of the
>block holder and how many µm the block moves towards the blade, but that is
>rubbish.
>René J.
>
>--- On Fri, 10/31/08, Dr. med. Frauke Neff <[EMAIL PROTECTED]>
>wrote:
>
>From: Dr. med. Frauke Neff <[EMAIL PROTECTED]>
>Subject: Re: [Histonet] Microtome calibration
>To: [EMAIL PROTECTED]
>Cc: histonet@lists.utsouthwestern.edu, [EMAIL PROTECTED]
>Date: Friday, October 31, 2008, 9:41 AM
>
>Dear Dr. Girish,
>I agree with rene, if the qualitiy of the cuts is fine it doesn't matter if
>they
> are 1µm or 2.3µm thick.
>But I'm highly interested in how your GLP auditors want to calibrate the
>thickness of the section cuts. Are you supposed to measure the slides after
>cutting?! Before or after stretching in the water bath?! If they have a
>protocol how to do this I would be happy if they can share it with us.
>
>Frauke
>
>
>Quoting Rene J Buesa <[EMAIL PROTECTED]>:
>
>> Dr. Girish:
>>
>> BOTH "requirements" are rubbish invented by bureaucrats with
>lots of time in
>> their hands and trying to appear concerned and knowledgeable.
>>
>> Thickness is not necessary as long as the section is diagnostically useful
>or
>> if some quantitative method as to the intensity is done in which case
>> thickness = amount of matter, and would influence the outcome of the
>> quantitative process.
>>
>> Timing the tissue processor is also totally irrelevant because it does not
>> really matter a fe

RE: [Histonet] Formalin and Xylene Monitoring Badges

2008-10-31 Thread Joyce Cline
Sensors Safety Products
Raleigh, N.C.
1-800-499-7232

Joyce Cline, H.T. (ASCP)
Technical Specialist
Hagerstown Medical Lab.
301-665-4980
fax 301-665-4941


-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Sandra
Cheasty
Sent: Friday, October 31, 2008 11:52 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formalin and Xylene Monitoring Badges

Happy Halloween...  I'm looking for a source of monitoring badges for
formalin and xylene, both the 8 hour TWA and the STEL. 

Thank you, Sandy

 

Sandra Cheasty

Histology Supervisor

UW-Madison 

School of Veterinary Medicine

608 263-1680

 

 

 

 

 

 

 



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RE: [Histonet] Formalin and Xylene Monitoring Badges

2008-10-31 Thread Michele Wich
Morphix Technologies
www.morphtec.com
757-431-2260

-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Kathleen
Boozer
Sent: Friday, October 31, 2008 11:22 AM
To: histonet@lists.utsouthwestern.edu; Sandra Cheasty
Subject: Re: [Histonet] Formalin and Xylene Monitoring Badges

ASSAY TECHNOLOGY
1252 Quarry Lane
Pleasanton, CA  94566
1-800-833-1258

>>> "Sandra Cheasty" <[EMAIL PROTECTED]> 10/31/2008 08:51 >>>
Happy Halloween...  I'm looking for a source of monitoring badges for
formalin and xylene, both the 8 hour TWA and the STEL.

Thank you, Sandy



Sandra Cheasty

Histology Supervisor

UW-Madison

School of Veterinary Medicine

608 263-1680

















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[Histonet] RE: Formalin and Xylene Monitoring Badges

2008-10-31 Thread Blazek, Linda
Try Mercedes Medical.
http://www.mercedesmedical.com/


Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email: [EMAIL PROTECTED]

 

-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Sandra Cheasty
Sent: Friday, October 31, 2008 11:52 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formalin and Xylene Monitoring Badges

Happy Halloween...  I'm looking for a source of monitoring badges for formalin 
and xylene, both the 8 hour TWA and the STEL. 

Thank you, Sandy

 

Sandra Cheasty

Histology Supervisor

UW-Madison 

School of Veterinary Medicine

608 263-1680

 

 

 

 

 

 

 



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Re: [Histonet] Formalin and Xylene Monitoring Badges

2008-10-31 Thread Kathleen Boozer
ASSAY TECHNOLOGY
1252 Quarry Lane
Pleasanton, CA  94566
1-800-833-1258 

>>> "Sandra Cheasty" <[EMAIL PROTECTED]> 10/31/2008 08:51 >>>
Happy Halloween...  I'm looking for a source of monitoring badges for formalin 
and xylene, both the 8 hour TWA and the STEL.

Thank you, Sandy



Sandra Cheasty

Histology Supervisor

UW-Madison

School of Veterinary Medicine

608 263-1680

















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[Histonet] Formalin and Xylene Monitoring Badges

2008-10-31 Thread Sandra Cheasty
Happy Halloween...  I'm looking for a source of monitoring badges for formalin 
and xylene, both the 8 hour TWA and the STEL.

Thank you, Sandy



Sandra Cheasty

Histology Supervisor

UW-Madison

School of Veterinary Medicine

608 263-1680

















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[Histonet] Polyclonal Proteolipid Protein Ab?

2008-10-31 Thread Patten, Nicole (NIH/NIAAA) [F]
I have been (unsuccessfully) trying to find a polyclonal antibody
against human proteolipid protein (stains myelin) that will work with
immunofluorescence on FFPE sections. I have also tried some myelin basic
protein with no luck. Has anyone found any in the past that have
worked?? 
Thanks!

 

Nicole J. Patten
Post-Baccalaureate Fellow/IRTA
NIAAA/National Institutes of Health

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RE: [Histonet] Microtome calibration

2008-10-31 Thread Pamela Marcum
We have a GLP person who is not familiar with Histology at all.  We have had
to educate her about these issues and may other things.  Calibration finally
came down to having a PM on the units every year.  After she understood all
the things we do with a section during the cutting phase and pick up that
would alter a true measurement. 

We also do MMA sections on the microtome and even those are next to
impossible to measure due to some stretching of the section during pick up
and drying.  Oh Yes, the drying of the slides really put her in a tail spin
as this was just not allowing the sections to stay the same as when they
were picked up.  

Sometimes a long talk and demonstration is the only way to make the point
that we are not standardized like clinical chemistry etc.  

Pamela A Marcum
University of Pennsylvania 
School of Veterinary Medicine
Comparative Orthopedic Laboratory (CORL)
382 W Street Rd
Kennett Square PA 19438
610-925-6278

-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Rene J Buesa
Sent: Friday, October 31, 2008 10:08 AM
To: Dr. med. Frauke Neff
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome calibration

Frauke:
That calibration is ussually done by measuring the advance mechanism of the
block holder and how many µm the block moves towards the blade, but that is
rubbish.
René J.

--- On Fri, 10/31/08, Dr. med. Frauke Neff <[EMAIL PROTECTED]>
wrote:

From: Dr. med. Frauke Neff <[EMAIL PROTECTED]>
Subject: Re: [Histonet] Microtome calibration
To: [EMAIL PROTECTED]
Cc: histonet@lists.utsouthwestern.edu, [EMAIL PROTECTED]
Date: Friday, October 31, 2008, 9:41 AM

Dear Dr. Girish,
I agree with rene, if the qualitiy of the cuts is fine it doesn't matter if
they
 are 1µm or 2.3µm thick.
But I'm highly interested in how your GLP auditors want to calibrate the
thickness of the section cuts. Are you supposed to measure the slides after
cutting?! Before or after stretching in the water bath?! If they have a
protocol how to do this I would be happy if they can share it with us.

Frauke


Quoting Rene J Buesa <[EMAIL PROTECTED]>:

> Dr. Girish:
>
> BOTH "requirements" are rubbish invented by bureaucrats with
lots of time in
> their hands and trying to appear concerned and knowledgeable.
>
> Thickness is not necessary as long as the section is diagnostically useful
or
> if some quantitative method as to the intensity is done in which case
> thickness = amount of matter, and would influence the outcome of the
> quantitative process.
>
> Timing the tissue processor is also totally irrelevant because it does not
> really matter a few minutes each way during a processing protocol. More
> important would be to keep a record of the fixation time that is the only
> step really critical in tissue processing.
>
> René J.
>
> --- On Fri, 10/31/08, [EMAIL PROTECTED] <[EMAIL PROTECTED]> wrote:
>
> From: [EMAIL PROTECTED] <[EMAIL PROTECTED]>
> Subject: [Histonet] Microtome calibration
> To: histonet@lists.utsouthwestern.edu
> Date: Friday, October 31, 2008, 2:49 AM
>
>
>
> Dear all
>
> Is anyone practising microtome calibration for thickness of sections cut?
> Our GLP auditors are frequently asking for it.
> They also suggest that automatic tissue processor time in each reagent
> should be calibrated.
>
> any comments
>
> Regards
> Dr Girish
> India
>
>
>
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>



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Re: [Histonet] Microtome calibration

2008-10-31 Thread Rene J Buesa
Frauke:
That calibration is ussually done by measuring the advance mechanism of the 
block holder and how many µm the block moves towards the blade, but that is 
rubbish.
René J.

--- On Fri, 10/31/08, Dr. med. Frauke Neff <[EMAIL PROTECTED]> wrote:

From: Dr. med. Frauke Neff <[EMAIL PROTECTED]>
Subject: Re: [Histonet] Microtome calibration
To: [EMAIL PROTECTED]
Cc: histonet@lists.utsouthwestern.edu, [EMAIL PROTECTED]
Date: Friday, October 31, 2008, 9:41 AM

Dear Dr. Girish,
I agree with rene, if the qualitiy of the cuts is fine it doesn't matter if
they
 are 1µm or 2.3µm thick.
But I'm highly interested in how your GLP auditors want to calibrate the
thickness of the section cuts. Are you supposed to measure the slides after
cutting?! Before or after stretching in the water bath?! If they have a
protocol how to do this I would be happy if they can share it with us.

Frauke


Quoting Rene J Buesa <[EMAIL PROTECTED]>:

> Dr. Girish:
>
> BOTH "requirements" are rubbish invented by bureaucrats with
lots of time in
> their hands and trying to appear concerned and knowledgeable.
>
> Thickness is not necessary as long as the section is diagnostically useful
or
> if some quantitative method as to the intensity is done in which case
> thickness = amount of matter, and would influence the outcome of the
> quantitative process.
>
> Timing the tissue processor is also totally irrelevant because it does not
> really matter a few minutes each way during a processing protocol. More
> important would be to keep a record of the fixation time that is the only
> step really critical in tissue processing.
>
> René J.
>
> --- On Fri, 10/31/08, [EMAIL PROTECTED] <[EMAIL PROTECTED]> wrote:
>
> From: [EMAIL PROTECTED] <[EMAIL PROTECTED]>
> Subject: [Histonet] Microtome calibration
> To: histonet@lists.utsouthwestern.edu
> Date: Friday, October 31, 2008, 2:49 AM
>
>
>
> Dear all
>
> Is anyone practising microtome calibration for thickness of sections cut?
> Our GLP auditors are frequently asking for it.
> They also suggest that automatic tissue processor time in each reagent
> should be calibrated.
>
> any comments
>
> Regards
> Dr Girish
> India
>
>
>
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>



This message was sent using IMP, the Internet Messaging Program.





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Re: [Histonet] Microtome calibration

2008-10-31 Thread Dr. med. Frauke Neff

Dear Dr. Girish,
I agree with rene, if the qualitiy of the cuts is fine it doesn't matter if they
 are 1µm or 2.3µm thick.
But I'm highly interested in how your GLP auditors want to calibrate the
thickness of the section cuts. Are you supposed to measure the slides after
cutting?! Before or after stretching in the water bath?! If they have a
protocol how to do this I would be happy if they can share it with us.

Frauke


Quoting Rene J Buesa <[EMAIL PROTECTED]>:

> Dr. Girish:
>
> BOTH "requirements" are rubbish invented by bureaucrats with lots of time in
> their hands and trying to appear concerned and knowledgeable.
>
> Thickness is not necessary as long as the section is diagnostically useful or
> if some quantitative method as to the intensity is done in which case
> thickness = amount of matter, and would influence the outcome of the
> quantitative process.
>
> Timing the tissue processor is also totally irrelevant because it does not
> really matter a few minutes each way during a processing protocol. More
> important would be to keep a record of the fixation time that is the only
> step really critical in tissue processing.
>
> René J.
>
> --- On Fri, 10/31/08, [EMAIL PROTECTED] <[EMAIL PROTECTED]> wrote:
>
> From: [EMAIL PROTECTED] <[EMAIL PROTECTED]>
> Subject: [Histonet] Microtome calibration
> To: histonet@lists.utsouthwestern.edu
> Date: Friday, October 31, 2008, 2:49 AM
>
>
>
> Dear all
>
> Is anyone practising microtome calibration for thickness of sections cut?
> Our GLP auditors are frequently asking for it.
> They also suggest that automatic tissue processor time in each reagent
> should be calibrated.
>
> any comments
>
> Regards
> Dr Girish
> India
>
>
>
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>



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[Histonet] anti-mouse ferritin

2008-10-31 Thread Kim Merriam
Hi,
 
Any recommedations for anti-mouse ferritin antibodies (looking to stain FFPE 
mouse tissue).
 
Thanks,
Kim

Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA



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[Histonet] pronase vs. proteinase-k

2008-10-31 Thread Kimberly Tuttle
Are they the same ?

Kimberly C. Tuttle  HT (ASCP)
Pathology Biorepository and Research Core
University of Maryland 
Room NBW58, UMMC
22 S. Greene St
Baltimore, MD 21201
(410) 328-5524
(410) 328-5508 fax 


 

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Re: [Histonet] IHC equipment

2008-10-31 Thread godsgalnow
This is a matter of opinion.? DO you like a closed system or an open system?? 
My preference is an open system and I prefer Biocare.? As for the PIN-4 
Cocktail, Biocare wins hands down there as well...as Dr Tacha works there.
-Original Message-
From: richard wells <[EMAIL PROTECTED]>
To: histonet 
Sent: Thu, 30 Oct 2008 2:17 pm
Subject: [Histonet] IHC equipment



I am helping to set up an in office lab for a group of urologists.? What is the 
best instrumentation for prostate tripple stain?? What are the best reagents?
?
Test volume will be relatively low.? Perhaps as many as 5 additional 
immunostains will be added in the future.? Testing will be done by an 
experienced histotech with minimal exposure to immunoperoxidase techniques.
?
Goals are to maximize simplicity, dependability and value.
?
Thanks in advance for any and all advice.
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Re: [Histonet] Microtome calibration

2008-10-31 Thread Rene J Buesa
Dr. Girish:
 
BOTH "requirements" are rubbish invented by bureaucrats with lots of time in 
their hands and trying to appear concerned and knowledgeable.
 
Thickness is not necessary as long as the section is diagnostically useful or 
if some quantitative method as to the intensity is done in which case thickness 
= amount of matter, and would influence the outcome of the quantitative process.
 
Timing the tissue processor is also totally irrelevant because it does not 
really matter a few minutes each way during a processing protocol. More 
important would be to keep a record of the fixation time that is the only step 
really critical in tissue processing.
 
René J.

--- On Fri, 10/31/08, [EMAIL PROTECTED] <[EMAIL PROTECTED]> wrote:

From: [EMAIL PROTECTED] <[EMAIL PROTECTED]>
Subject: [Histonet] Microtome calibration
To: histonet@lists.utsouthwestern.edu
Date: Friday, October 31, 2008, 2:49 AM



Dear all

Is anyone practising microtome calibration for thickness of sections cut?
Our GLP auditors are frequently asking for it.
They also suggest that automatic tissue processor time in each reagent
should be calibrated.

any comments

Regards
Dr Girish
India





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[Histonet] PIN-4 controls

2008-10-31 Thread Sharon Campbell
Happy Halloween!

Does anyone know of a good PIN-4 control? We are currently using
in-house tissue but are finding a lot of variance with the stainability.
If anyone knows of a good source I would greatly appreciate it.

Thanks in advance,

 

 

Sharon Campbell, HTL(ASCP)CM, BSBM

Histology Supervisor

Celligent Diagnostics, LLC

Formerly Pathology Associates Services

101 East W.T. Harris Blvd, Suite 1212

Charlotte, NC  28262

(704) 549-8444 x104

[EMAIL PROTECTED]

 

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