[Histonet] Paraffin wax
Hello, Could someone share their knowledge of the Paraplast line of paraffin with me? We are being forced to change from the TissuePrep line. Thanks Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin wax
I have had very good luck with Paraplast Plus for infiltration and using the Paraplast Xtra for embedding. On Thu, Dec 11, 2008 at 10:00 AM, Charles, Roger [EMAIL PROTECTED]wrote: Hello, Could someone share their knowledge of the Paraplast line of paraffin with me? We are being forced to change from the TissuePrep line. Thanks Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin wax
I always used Paraplast and I consider it as the best paraffin wax there is. René J. --- On Thu, 12/11/08, Charles, Roger [EMAIL PROTECTED] wrote: From: Charles, Roger [EMAIL PROTECTED] Subject: [Histonet] Paraffin wax To: Histonet (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Date: Thursday, December 11, 2008, 10:00 AM Hello, Could someone share their knowledge of the Paraplast line of paraffin with me? We are being forced to change from the TissuePrep line. Thanks Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Billing question
It is my understand that , Yes you can bill as long as the ASR disclaimer is on the report and you heave validation of such antibodies that do did not have FDA approvals yet Michael LaFriniere On 12/9/2008 at 12:53:16 PM, Rene J Buesa [EMAIL PROTECTED] wrote: As your pathologists first because even when we charged for IHC to the patients, there was always a disclaimer saying that the Ab was for research, in spite of which, the charges were done (they are essentially for the pathologist interpretation).Hope this will help you. René J. --- On Tue, 12/9/08, KELLY BOYD [EMAIL PROTECTED] wrote: From: KELLY BOYD [EMAIL PROTECTED] Subject: [Histonet] Billing question To: histonet Histonet@lists.utsouthwestern.edu Date: Tuesday, December 9, 2008, 12:15 PM Hi all! Question for those familiar with all the billing regulations: If you use an antibody that is for RUO (research use only) or ASR (analyte specific reagent), can you bill the patient for these immunos? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Offended
I Wanted to obtained opinions of Managers/Supervisors in the histo world and how you handle situations regarding recruiters calling directly into Pathology Labs to specific tech's and stating they are seeking referrals for job placements. I recently had an AP staffing solution company call one of my labs to talk to any histologist I felt was to recruit staff. I feel this is an unprofessional practice to call directly into the laboratory and to hide behind the wording referrals when actually it appears they are trying to get information from the tech on the phone seeing if they are looking for a new job. I feel that this is an intrusion as well as inappropriate in our line of business and we are not in the business to give referrals to companies in the staffing solution arena for their monetary gain as well as trying to entice our staff. What the staff does with these types of companies on personal time is their business, however, I think it is offending for companies to demonstrate this behavior. If anyone would like to know which company and person called into my lab I will personally inform you on private email! Michael LaFriniere Executive Director CSM Washington DC/Maryland/Virginia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] PCNA staining on paraffin
I use Dako's PCNA (cat. # M0789), mouse monoclonal, clone PC10, HIER with pressure cooker (Decloaker, 30 at 121C, 21psi) in Target Retrieval buffer, 45 min in primary antibody (current dilution is 1:200, but that varies with each new lot), 30 min in goat anti-Ms EnVision+/HRP, 15 min in AEC. I use an autostainer, but it can be done manually with the same incubation times at RT. This works for me on many mammalian species. Jan Shivers UMN VetDiagLab St. Paul, MN - Original Message - From: Merced Leiker [EMAIL PROTECTED] To: histonet@lists.utsouthwestern.edu Sent: Wednesday, December 10, 2008 1:07 PM Subject: [Histonet] PCNA staining on paraffin Does anyone have any suggestions for staining PCNA on paraffin? We are using Santa Cruz's PCNA (FL261) SC-7907. We have already stained with it successfully on frozen sections, but it does not appear to be working on paraffin. Thank you. Merced M Leiker Research Technician II 354 BRB (pkgs) / 140 Farber Hall (letters) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 Without my flaws I'm really very boring. - random internet blog commentator ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] PCNA staining on paraffin
Second this, the DAKO clone works great. -Original Message- From: Jan Shivers [EMAIL PROTECTED] Date: Thu, 11 Dec 2008 10:01:38 To: Merced Leiker[EMAIL PROTECTED] Cc: histonethistonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PCNA staining on paraffin I use Dako's PCNA (cat. # M0789), mouse monoclonal, clone PC10, HIER with pressure cooker (Decloaker, 30 at 121C, 21psi) in Target Retrieval buffer, 45 min in primary antibody (current dilution is 1:200, but that varies with each new lot), 30 min in goat anti-Ms EnVision+/HRP, 15 min in AEC. I use an autostainer, but it can be done manually with the same incubation times at RT. This works for me on many mammalian species. Jan Shivers UMN VetDiagLab St. Paul, MN - Original Message - From: Merced Leiker [EMAIL PROTECTED] To: histonet@lists.utsouthwestern.edu Sent: Wednesday, December 10, 2008 1:07 PM Subject: [Histonet] PCNA staining on paraffin Does anyone have any suggestions for staining PCNA on paraffin? We are using Santa Cruz's PCNA (FL261) SC-7907. We have already stained with it successfully on frozen sections, but it does not appear to be working on paraffin. Thank you. Merced M Leiker Research Technician II 354 BRB (pkgs) / 140 Farber Hall (letters) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 Without my flaws I'm really very boring. - random internet blog commentator ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Silly Question?
Hi Pat, Paraformaldehyde does not contain any additives and is considered more pure than formaldehyde which often contains methanol which in some cases is undesirable depending on the type of assay being conducted. Linda Pat Flannery wrote: Please humor me on this if it's obvious (to everyone but me): why do we use paraformaldehyde (which is so inconvenient to make up) rather than buffered formalin or just diluted formaldehyde itself? It seems that around here, some folks prefer paraformaldehyde (either 2% or 4%) and others use formalin, while some others stick to diluted formaldehyde (I see all 4 on labels for specimens submitted for histology). Is it mostly a matter of personal preference or where you were trained (i.e. force of habit) or is there a valid reason to use each solution (basically the same chemical once in solution, merely buffered or not)? The only answer I've gotten when I've asked is, That's what we always use. Thanks. -Pat Flannery (not a real histologist - I just play one in the lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Silly Question? - Need help quickly!
I was just going to post a question regarding paraformaldhyde myself! Just last week I believe I remember someone saying that paraformaldehyde and formalin are the same and they had put the same solution in two different containers for one of their researchers because they were so insistent to have two different solutions. Are they the same? Well, today I have a request to put tissue for a researcher in formalin and paraformaldehyde. So Without percentage required, do I use 10% NBF? Do I call somewhere and get paraformaldehyde and make 4% paraformaldehyde? I have asked the surgeon twice for the number for the lab so I can find out - don't have it yet. I have two fresh adrenals in the fridge. Help!! Thanks in advance... Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Pat Flannery Sent: Thursday, December 11, 2008 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Silly Question? Please humor me on this if it's obvious (to everyone but me): why do we use paraformaldehyde (which is so inconvenient to make up) rather than buffered formalin or just diluted formaldehyde itself? It seems that around here, some folks prefer paraformaldehyde (either 2% or 4%) and others use formalin, while some others stick to diluted formaldehyde (I see all 4 on labels for specimens submitted for histology). Is it mostly a matter of personal preference or where you were trained (i.e. force of habit) or is there a valid reason to use each solution (basically the same chemical once in solution, merely buffered or not)? The only answer I've gotten when I've asked is, That's what we always use. Thanks. -Pat Flannery (not a real histologist - I just play one in the lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Silly Question?
Thank you, Jeanine, Joyce, and Linda. The tissues I'm cutting are just for HE staining and light microscopy; they won't be used for Immunostaining, antigen retrieval, or anything too fancy. I don't see what difference it would make, but of course I'll put them in whatever people ask for. I know that commercial formaldehyde is stabilized for storage so I can understand some folks wouldn't want the MeOH contamination, no matter how slight. -Pat ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] training materials
I would be very interested in these suggestions as well, as we would like to improve the quality of skin embedding. Thanks! On Thu, Dec 11, 2008 at 10:59 AM, Jennifer Johnson [EMAIL PROTECTED]wrote: Can anyone suggest a really good book, atlas, etc. for embedding? The girl that took my place at my last job is having a really hard time (especially with skin) and I told her I would ask the experts. Thanks, Jennifer Johnson, HTL (ASCP) _ Send e-mail faster without improving your typing skills. http://windowslive.com/Explore/hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_speed_122008___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Silly Question?
The advantage of para-formaldehyde is that you can prepare more easily any type of formaldehyde solution, at any concentration and also it does not have any additives (methanol) as pure formalin (37-50%formaldehydede) does. In reality the fixation mechanism is the same and sometimes the whole issue boils down to personal preferences. René J. --- On Thu, 12/11/08, Pat Flannery pjfne...@duke.edu wrote: From: Pat Flannery pjfne...@duke.edu Subject: [Histonet] Silly Question? To: histonet@lists.utsouthwestern.edu Date: Thursday, December 11, 2008, 11:58 AM Please humor me on this if it's obvious (to everyone but me): why do we use paraformaldehyde (which is so inconvenient to make up) rather than buffered formalin or just diluted formaldehyde itself? It seems that around here, some folks prefer paraformaldehyde (either 2% or 4%) and others use formalin, while some others stick to diluted formaldehyde (I see all 4 on labels for specimens submitted for histology). Is it mostly a matter of personal preference or where you were trained (i.e. force of habit) or is there a valid reason to use each solution (basically the same chemical once in solution, merely buffered or not)? The only answer I've gotten when I've asked is, That's what we always use. Thanks. -Pat Flannery (not a real histologist - I just play one in the lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] training materials
The AFIP book (Laboratory Methods in Histotechnology, edited by Edna Prophet, et al) has nice chapters on specimen orientation and embedding It is not really a training manual, but it has some nice pictures as to how certain tissues should be placed into the molds. Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Daniel Schneider dlschnei...@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Thursday, December 11, 2008 12:20:40 PM Subject: Re: [Histonet] training materials I would be very interested in these suggestions as well, as we would like to improve the quality of skin embedding. Thanks! On Thu, Dec 11, 2008 at 10:59 AM, Jennifer Johnson jmjohnso...@hotmail.comwrote: Can anyone suggest a really good book, atlas, etc. for embedding? The girl that took my place at my last job is having a really hard time (especially with skin) and I told her I would ask the experts. Thanks, Jennifer Johnson, HTL (ASCP) _ Send e-mail faster without improving your typing skills. http://windowslive.com/Explore/hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_speed_122008___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Silly Question? - Need help quickly!
So...is a polymer of paraformaldehyde considered depolymerized if it remains somewhere between 1-50 molecules long once it's been dissolved in solution, however it's dissolved? (the dissolving being a separate topic of debate on Histonet). Does it matter for tissue fixation purposes if there are formaldehyde chain lengths of 50 molecules present in solution - not long enough to precipitate out, but perhaps long enough to affect its penetration and fixing of tissues? Any ideas? Merced --On Thursday, December 11, 2008 9:58 AM -0800 Rene J Buesa rjbu...@yahoo.com wrote: Joyce: Methanal, which is the chemical name of formaldehyde, polymerizes. If it forms a polymer of at least 50 molecules or more, it gets solid = para-formaldehyde. Formalin (a trade name as formol is also another trade name)is the 37-50% aqueous solution of formaldehyde (with some additiveses to prevent polymerization). You can prepare BNF using the formalin solution or dissolving the amount of solid para-formaldehydede to get to the concentrationon you desire. The chemical in both solutions is the same = methanal or formaldehyde.René J. --- On Thu, 12/11/08, Weems, Joyce jwe...@sjha.org wrote: From: Weems, Joyce jwe...@sjha.org Subject: RE: [Histonet] Silly Question? - Need help quickly! To: Pat Flannery pjfne...@duke.edu, histonet@lists.utsouthwestern.edu Date: Thursday, December 11, 2008, 12:12 PM I was just going to post a question regarding paraformaldhyde myself! Just last week I believe I remember someone saying that paraformaldehyde and formalin are the same and they had put the same solution in two different containers for one of their researchers because they were so insistent to have two different solutions. Are they the same? Well, today I have a request to put tissue for a researcher in formalin and paraformaldehyde. So Without percentage required, do I use 10% NBF? Do I call somewhere and get paraformaldehyde and make 4% paraformaldehyde? I have asked the surgeon twice for the number for the lab so I can find out - don't have it yet. I have two fresh adrenals in the fridge. Help!! Thanks in advance... Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pat Flannery Sent: Thursday, December 11, 2008 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Silly Question? Please humor me on this if it's obvious (to everyone but me): why do we use paraformaldehyde (which is so inconvenient to make up) rather than buffered formalin or just diluted formaldehyde itself? It seems that around here, some folks prefer paraformaldehyde (either 2% or 4%) and others use formalin, while some others stick to diluted formaldehyde (I see all 4 on labels for specimens submitted for histology). Is it mostly a matter of personal preference or where you were trained (i.e. force of habit) or is there a valid reason to use each solution (basically the same chemical once in solution, merely buffered or not)? The only answer I've gotten when I've asked is, That's what we always use. Thanks. -Pat Flannery (not a real histologist - I just play one in the lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 BRB (pkgs) / 140 Farber Hall (letters) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 Without my flaws I'm really very boring. - random internet blog commentator ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] perfusion question
The wash-out solution should have pH and osmotic pressure close to those of the animal's extracellular fluid, to avoid shrinkage or swelling of cells, collagen fibres etc. This can be achieved with simple saline (0.9% NaCl). A buffer prevents acidification of the extracellular fluid by products released from dying cells. Calcium ions (not compatible with phosphate buffers) enhance the preservation of phospholipids of cell membranes, myelin etc. Potassium ions are included in physiological saline solutions such as Ringer-Locke in which tissues and small organs can be kept alive, sometimes for several hours. I don't know of any study of effects of potassium on fixation, but probably someone has looked into it. The formaldehyde should also be dissolved in an isosmotic buffer because the chemical events of fixation occur slowly (several hours). Brain tissue still responds to changes in ambient osmotic pressure after several hours in neutral buffered formaldehyde. In glutaraldehyde, however, the cells are stabilized in 20 minutes. See: Paljarvi L, Garcia JH, Kalimo H (1979) The efficiency of aldehyde fixation for electron microscopy: stabilization of rat brain tissue to withstand osmotic stress. Histochem. J. 11: 267-276. This paper has also has references to several other studies. Traditional fixative mixtures are mostly acidic and rapidly acting, stabilizing the structure of the tissue (for light microscopy) before the development of adverse effects of low pH or osmotic pressure. The subject was also reviewed by J.R.Baker in his book Principles of Biological Microtechnique (1958), pp.75-86. John Kiernan Anatomy, UWO = = = - Original Message - From: Neil Fournier nfourn...@sasktel.net Date: Wednesday, December 10, 2008 14:42 Subject: [Histonet] perfusion question To: histonet@lists.utsouthwestern.edu Is there a rationale for using normal saline (0.9% (w/v) NaCl dissolved in dH2O) over 0.1 M PBS (pH 7.4) as a rinsing solution during intraventricular perfusion of a rat. Would one yield better results over the other? Also is there a raionale for why some people perfuse using PBS made only from monobasic and dibasic sodium phosphate (with 0.9% NaCl) vs. using PBS that also include KCl, sodium phosphate dibasic, NaCl, and potassium phosphate monobasic in the recipe. Thanks for the help Neil ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Original Message - From: Neil Fournier nfourn...@sasktel.net Date: Wednesday, December 10, 2008 14:42 Subject: [Histonet] perfusion question To: histonet@lists.utsouthwestern.edu Is there a rationale for using normal saline (0.9% (w/v) NaCl dissolved in dH2O) over 0.1 M PBS (pH 7.4) as a rinsing solution during intraventricular perfusion of a rat. Would one yield better results over the other? Also is there a raionale for why some people perfuse using PBS made only from monobasic and dibasic sodium phosphate (with 0.9% NaCl) vs. using PBS that also include KCl, sodium phosphate dibasic, NaCl, and potassium phosphate monobasic in the recipe. Thanks for the help Neil ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] paraffin processing rat tissue
Dear Histo-Experts, I'm interested in paraffin processing protocols for rat kidneys, spleens and livers. Specifically, I'm interested in fixation time (immersion fixation in formalin) and dehydration and paraffin infiltration times. I've done some using my best guess based on the thickness of the tissue and resulting sections (5-7um) have spaces in them (after H and E) which may be due to inadequate fixation or dehydration times (I'm guessing). The spaces don't look like tears from sectioning...they look more like tissue separation... If you wouldn't mind sharing your protocols with me that would be WONDERFUL!!! A thousand thanks in advance! danielle Danielle Crippen Morphology and Imaging Core ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: PCNA
Use a PC10 mouse clone for pwax sections: works very well on many species that have been fixed in routine Formalin. Imho, no special procedures reqd. Sure, AR is best. If you wish to check out this site: http://www.immunoportal.com/index.php you might find further help? Also , be very careful if you use PCNA as a proliferation marker..it has a very long half-life compared with Ki67. So, use an anti Ki67 Ab instead for general proliferation studies that cannot include use of BrdU. Sure, if you want to assess a Mitotic index...use pH3 Ab on pwax sections. carl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Offended
A certain company has recently started sending histo related fliers TO MY HOUSE. I want to know where they got my home address, especially since I have never actually done business with this company. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of JR R Sent: Thu 12/11/2008 11:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Offended Hi Michael, Yeah, I can see how that would be annoying to you. If you get a solicitation call at work that bugs you--tell them to buzz off. I don't know that you ought to let it hurt your feelings or something, though. In the world of business, it is reasonable to expect that head hunters are at all times seeking to hire your best and brightest out from under you--the way to stop them is by making it worth people's while to stick around. To my knowledge, histotechnologists are, as a rule of thumb, over-worked and under-appreciated. And yet they are darn handy to have around. I suggest that you make it your personal mission to ensure that your histotechs ARE NOT looking for another job. That way, when a recruiter calls, your techs say Leave my current position? What are you crazy??!!! Jerry Ricks Research Scientist University of Washington Department of Pathology. Date: Thu, 11 Dec 2008 10:37:42 -0500 From: mlafr...@csmlab.com To: Histonet@lists.utsouthwestern.edu CC: Subject: [Histonet] Offended I Wanted to obtained opinions of Managers/Supervisors in the histo world and how you handle situations regarding recruiters calling directly into Pathology Labs to specific tech's and stating they are seeking referrals for job placements. I recently had an AP staffing solution company call one of my labs to talk to any histologist I felt was to recruit staff. I feel this is an unprofessional practice to call directly into the laboratory and to hide behind the wording referrals when actually it appears they are trying to get information from the tech on the phone seeing if they are looking for a new job. I feel that this is an intrusion as well as inappropriate in our line of business and we are not in the business to give referrals to companies in the staffing solution arena for their monetary gain as well as trying to entice our staff. What the staff does with these types of companies on personal time is their business, however, I think it is offending for companies to demonstrate this behavior. If anyone would like to know which company and person called into my lab I will personally inform you on private email! Michael LaFriniere Executive Director CSM Washington DC/Maryland/Virginia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Send e-mail faster without improving your typing skills. http://windowslive.com/Explore/hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_speed_122008___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: processing mouse seminal vesicles
Try this processing schudule for mouse tissue: 70% 15 min 95% 15 min 95% 15 min 100% 15 min 100% 15 min 100% 15 min 100% 15 min Xylene 15 min Xylene 15 min Paraffin 15 min Paraffin 15 min Paraffin 15 min Paraffin 15 min Good luck! Susan Bincsik Does anybody have a protocol for this? My last batch of these came out VERY dry and crunchy when run with other tissues on my standard protocol, which is as follows: (They are fixed on the benchtop in 10% NBF for 4-5 days, then rinsed out before processing.) 70%: 30 min 80%: 30 min 95%: 45 min 95%: 45 min 100%: 45 min 100%: 45 min xylene: 45 min xylene: 45 min Paraffin: 30 min Paraffin: 30min Paraffin: 30 min My other thought is that something is up with our VIP 5 processor, though no error messages are showing up. Any and all suggestions are most welcome. Thanks in advance, Kathleen Roberts Rutgers University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Silly Question?
Hi Pat: The differences are largely in the minds of the investigators. Confusion comes from inexact nomenclature. One part of formaldehyde (37-40%) plus 9 parts of buffer makes formalin or 10% formalin which is about 4% formaldehyde. Yes, the 37-40% formaldehyde you buy has some methanol added to keep it from polymerizing but it makes no difference in the quality of fixation, especially given the (too) short times used to fix clinical specimens. Sure, you can go to the trouble to make 4% formaldehyde solution fresh from paraformaldehyde but for the vast majority of applications it just does not matter. Geoff Pat Flannery wrote: Please humor me on this if it's obvious (to everyone but me): why do we use paraformaldehyde (which is so inconvenient to make up) rather than buffered formalin or just diluted formaldehyde itself? It seems that around here, some folks prefer paraformaldehyde (either 2% or 4%) and others use formalin, while some others stick to diluted formaldehyde (I see all 4 on labels for specimens submitted for histology). Is it mostly a matter of personal preference or where you were trained (i.e. force of habit) or is there a valid reason to use each solution (basically the same chemical once in solution, merely buffered or not)? The only answer I've gotten when I've asked is, That's what we always use. Thanks. -Pat Flannery (not a real histologist - I just play one in the lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] posting for Tim
Subject: respond directly to Tim on this atnbs= p;[1]tmal...@cox.net HELP ! HISTONET ADDRESS From: Timothy Malloy [2]tmal...@cox.net ([3]Add as Preferred Sender) [4] 3D? = /TD Date: Thu, Dec 11, 2008 2:57 am To: [5]pru...@ihctech.net, [6]tmallo...@hotm= ail.com, [7]tmal...@cox.net Hi Patsy, I am a fan of yours on histonet. Just recently I moved my mail fr= om hotmail to outlook and lost histonets address. Could you supply me with = this request. I want to post a question . maybe you would be likely to answ= er this one. We work for the providence RI VA Hospital and we are in need o= f a procedure for GMS using a hot plate instead of a microwave. We have iss= ues venting the microwave because of the age of the building. Would you kno= w of a silver stain to test for pneumocystisis on a hot plate?? Thank You Tim Malloy [8]tmal...@cox.net or [9]tmallo...@hotmail.= com Timothy Malloy HT (ASCP) A.A.S. References 1. 3Dhttp://em=/ 2. 3Dhttp://email.secureserver.net/addressBookQuickAdd.php?contact 3. 3Dhttp://email.secureserver.net/w 4. 3Dhttp://email.secureserver.net/webm 5. 3Dhttp://email.secureserver.net/addressBookQuickAdd.php?cont 6. 3Dhttp://email.secureserver.net/addr 7. 3Dhttp://emai=/ 8. file://localhost/tmp/3Dm 9. 3Dmailto:tmallo...@hotmail.com; ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AxioVision
Hi, Is any one using Zeiss AxioVision software to analyze images for histomorphometery? Shakun P. Aswani Scientist I, Preclinical Development Acologix, Inc. 3960 Point Eden Way Hayward CA 94545 (510) 512-7231 phone (510) 786-1116 facsimile shakun.asw...@acologix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] AxioVision
We have used Automeasure from Zeiss in the past for image analysis. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 l...@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shakun Aswani Sent: Thursday, December 11, 2008 2:17 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] AxioVision Hi, Is any one using Zeiss AxioVision software to analyze images for histomorphometery? Shakun P. Aswani Scientist I, Preclinical Development Acologix, Inc. 3960 Point Eden Way Hayward CA 94545 (510) 512-7231 phone (510) 786-1116 facsimile shakun.asw...@acologix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] glutaraldehyde autofluoresence
Hi, My glutaraldehyde fixed mouse embryos fluoresce slightly reddish orange under rhodamine. I have read on this website's archives that using a solution of bland amino acids can help get rid of this. Does anyone have a protocol to fix this problem or can someone explain to me how and why this works? Eric Schmidt ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Offended
do you get any trade publications, by any chance? those people are the *worst* about selling your info... I signed up for one or two, now I get calls to my office almost every day for other publications, seminars and workshops that aren't even remotely relevant to my field... Guess its true there is in fact no free lunch these days... Ingles Claire wrote: A certain company has recently started sending histo related fliers TO MY HOUSE. I want to know where they got my home address, especially since I have never actually done business with this company. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of JR R Sent: Thu 12/11/2008 11:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Offended Hi Michael, Yeah, I can see how that would be annoying to you. If you get a solicitation call at work that bugs you--tell them to buzz off. I don't know that you ought to let it hurt your feelings or something, though. In the world of business, it is reasonable to expect that head hunters are at all times seeking to hire your best and brightest out from under you--the way to stop them is by making it worth people's while to stick around. To my knowledge, histotechnologists are, as a rule of thumb, over-worked and under-appreciated. And yet they are darn handy to have around. I suggest that you make it your personal mission to ensure that your histotechs ARE NOT looking for another job. That way, when a recruiter calls, your techs say Leave my current position? What are you crazy??!!! Jerry Ricks Research Scientist University of Washington Department of Pathology. Date: Thu, 11 Dec 2008 10:37:42 -0500 From: mlafr...@csmlab.com To: Histonet@lists.utsouthwestern.edu CC: Subject: [Histonet] Offended I Wanted to obtained opinions of Managers/Supervisors in the histo world and how you handle situations regarding recruiters calling directly into Pathology Labs to specific tech's and stating they are seeking referrals for job placements. I recently had an AP staffing solution company call one of my labs to talk to any histologist I felt was to recruit staff. I feel this is an unprofessional practice to call directly into the laboratory and to hide behind the wording referrals when actually it appears they are trying to get information from the tech on the phone seeing if they are looking for a new job. I feel that this is an intrusion as well as inappropriate in our line of business and we are not in the business to give referrals to companies in the staffing solution arena for their monetary gain as well as trying to entice our staff. What the staff does with these types of companies on personal time is their business, however, I think it is offending for companies to demonstrate this behavior. If anyone would like to know which company and person called into my lab I will personally inform you on private email! Michael LaFriniere Executive Director CSM Washington DC/Maryland/Virginia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Send e-mail faster without improving your typing skills. http://windowslive.com/Explore/hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_speed_122008___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Silly Question?
Where is the evidence - or is this another mythical beast some of us believe? I don't believe it. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Linda M Watson Sent: Friday, 12 December 2008 4:11 AM To: Pat Flannery Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Silly Question? Hi Pat, Paraformaldehyde does not contain any additives and is considered more pure than formaldehyde which often contains methanol which in some cases is undesirable depending on the type of assay being conducted. Linda Pat Flannery wrote: Please humor me on this if it's obvious (to everyone but me): why do we use paraformaldehyde (which is so inconvenient to make up) rather than buffered formalin or just diluted formaldehyde itself? It seems that around here, some folks prefer paraformaldehyde (either 2% or 4%) and others use formalin, while some others stick to diluted formaldehyde (I see all 4 on labels for specimens submitted for histology). Is it mostly a matter of personal preference or where you were trained (i.e. force of habit) or is there a valid reason to use each solution (basically the same chemical once in solution, merely buffered or not)? The only answer I've gotten when I've asked is, That's what we always use. Thanks. -Pat Flannery (not a real histologist - I just play one in the lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Silly Question?
And I won't stop!! I would prefer a slight methanol rather than no formaldehyde (see previous post) or formic acid being present. Also if some one sent me tissues fixed in a mercury containing fixative, I would report them and send it back. If researches or surgeons want quality results from my lab, they follow my rules or take a different road. But having said this I will negotiate, hoping to get the best quality that we can. Communication (and sometimes education) is the key Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pat Flannery Sent: Friday, 12 December 2008 4:22 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Silly Question? Thank you, Jeanine, Joyce, and Linda. The tissues I'm cutting are just for HE staining and light microscopy; they won't be used for Immunostaining, antigen retrieval, or anything too fancy. I don't see what difference it would make, but of course I'll put them in whatever people ask for. I know that commercial formaldehyde is stabilized for storage so I can understand some folks wouldn't want the MeOH contamination, no matter how slight. -Pat ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Silly Question? - Need help quickly!
Good point Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced Leiker Sent: Friday, 12 December 2008 5:19 AM To: rjbu...@yahoo.com; Pat Flannery; histonet@lists.utsouthwestern.edu; Weems, Joyce Subject: RE: [Histonet] Silly Question? - Need help quickly! So...is a polymer of paraformaldehyde considered depolymerized if it remains somewhere between 1-50 molecules long once it's been dissolved in solution, however it's dissolved? (the dissolving being a separate topic of debate on Histonet). Does it matter for tissue fixation purposes if there are formaldehyde chain lengths of 50 molecules present in solution - not long enough to precipitate out, but perhaps long enough to affect its penetration and fixing of tissues? Any ideas? Merced --On Thursday, December 11, 2008 9:58 AM -0800 Rene J Buesa rjbu...@yahoo.com wrote: Joyce: Methanal, which is the chemical name of formaldehyde, polymerizes. If it forms a polymer of at least 50 molecules or more, it gets solid = para-formaldehyde. Formalin (a trade name as formol is also another trade name)is the 37-50% aqueous solution of formaldehyde (with some additiveses to prevent polymerization). You can prepare BNF using the formalin solution or dissolving the amount of solid para-formaldehydede to get to the concentrationon you desire. The chemical in both solutions is the same = methanal or formaldehyde.René J. --- On Thu, 12/11/08, Weems, Joyce jwe...@sjha.org wrote: From: Weems, Joyce jwe...@sjha.org Subject: RE: [Histonet] Silly Question? - Need help quickly! To: Pat Flannery pjfne...@duke.edu, histonet@lists.utsouthwestern.edu Date: Thursday, December 11, 2008, 12:12 PM I was just going to post a question regarding paraformaldhyde myself! Just last week I believe I remember someone saying that paraformaldehyde and formalin are the same and they had put the same solution in two different containers for one of their researchers because they were so insistent to have two different solutions. Are they the same? Well, today I have a request to put tissue for a researcher in formalin and paraformaldehyde. So Without percentage required, do I use 10% NBF? Do I call somewhere and get paraformaldehyde and make 4% paraformaldehyde? I have asked the surgeon twice for the number for the lab so I can find out - don't have it yet. I have two fresh adrenals in the fridge. Help!! Thanks in advance... Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pat Flannery Sent: Thursday, December 11, 2008 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Silly Question? Please humor me on this if it's obvious (to everyone but me): why do we use paraformaldehyde (which is so inconvenient to make up) rather than buffered formalin or just diluted formaldehyde itself? It seems that around here, some folks prefer paraformaldehyde (either 2% or 4%) and others use formalin, while some others stick to diluted formaldehyde (I see all 4 on labels for specimens submitted for histology). Is it mostly a matter of personal preference or where you were trained (i.e. force of habit) or is there a valid reason to use each solution (basically the same chemical once in solution, merely buffered or not)? The only answer I've gotten when I've asked is, That's what we always use. Thanks. -Pat Flannery (not a real histologist - I just play one in the lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
[Histonet] Coverslipping Fume Hoods
Can anyone recommend a heavy-duty fume hood that we can use for coverslipping? We are currently using a Fume Guard hood that is probably 20 years old. We use HistoClear to coverslip, and we would like to cut down on the vapors/smell. Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Non-gyn cytology prep - amount processed?
Hi Cathy, Your message came through a little garbled, but I think I get the jist of it. Standard procedure for large volume cytology fluid specimens is to mix the specimen well, and then take off a 50 ml aliquot to centrifuge down and make the slides and cell block. If the specimen is not mixed, you are likely to miss the settled malignant cells. It is not prudent (or recommended!) to centrifuge down the entire specimen. If a specimen were extremely sparsely cellular, I might occasionally spin down two 50 ml aliquots. I agree that if a specimen has set for a while and settled naturally, pouring off the supernatent and using the concentrated precipitate for a 50 ml aliquat can be a nice bonus. BUT, there should have been malignant cells in the WELL-MIXED original aliquot. What prep procedures are you using for the slides?? ThinPrep processing can be subject to problems with these kind of specimens because sometimes the filter becomes 'clogged' with fibrin and protein, and the processor thinks it has cells, but not much is there. ThinPrep may not be the best choice for body fluid specimens. A well done cell block should also have shown cells in it. If you are simply scraping loose cells into a lens paper to process and try to embed for a cell block, I'm not surprised if you lost the cells and only recovered the more prominent fibrin. It's always a good idea to use some method to actually hold (block!) the cells together - such as agar, thrombin-prothrombin, or albumin clot. Beth Cox, SCT/HT(ASCP) _ Message: 3 Date: Thu, 11 Dec 2008 10:10:31 -0800 From: cathy.crump...@tuality.org Subject: [Histonet] Non-gyn cytology prep - amount processed? To: histonet@lists.utsouthwestern.edu Message-ID: of6ce74de0.44dc7092-on8825751c.0063d701-8825751c.0063d...@tuality.org Content-Type: text/plain; charset=ISO-8859-1 Hi all, for those of you who also work with cytology - what is procedure for fluids that have a large volume? How much of thefluid do you process? I am wanting to compare our polic instance this week where we received processed 100 mL of that after making sure it first cell blocks only contained fibrin, but the malignant cells. When we went back to the contai settled after 24 hours so we poured off the top part of t and only processed the gunk at the bottom. The second time t were malignant cells in the cell block. My pathologist is pushin for us to process 100% of the fluids, 100% of the time, saying what we cu get those ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Offended
Joe, I think firing a tech because a head hunter calls is unfair and maybe a case for a lawsuit of improper termination. Often the heah hunters get your name through the grapevine, from former employees or from publications. - Joe said: I told him that if he was looking for another job to do it on his time and on his dollar. If I ever received another call from a headhunter naming him, he would have to talk to them because he would be shown the door. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Non-gyn cytology prep - amount processed?
Cathy Crumpton asks (the message seems to have gotten garbled in transmission): Hi all, for those of you who also work with cytology - what is procedure for fluids that have a large volume? How much of thefluid do you process? - I am wanting to compare our polic instance this week where we received processed 100 mL of that after making sure it first cell blocks only contained fibrin, but the malignant cells. When we went back to the contai settled after 24 hours so we poured off the top part of t and only processed the gunk at the bottom. The second time t were malignant cells in the cell block. My pathologist is pushin for us to process 100% of the fluids, 100% of the time, saying what we cu get those You certainly cannot process an entire large liquid specimen for cytologic examination - many pleural fluid specimens are well over a litre. There are a number of ways to prepare a cell block from such a specimen - an important adjunct to the cytologic examination examination of such fluids. A lot of laboratories have trouble preparing cell blocks consistently. I'd suggested getting a method from a cytopathology service that has it working - and probably visiting the service. If your pathologist thinks you can process 100% of a one litre specimen, he isn't going to be of much help to you in getting a procedure working. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Non-gyn cytology prep
When doing cell blocks you should not process the fibrin tissue or what is floating on top. At least that has always been the preference of the pathologist's that I've worked with. They don't want to see the fibrin or the junk that's floating on top. You will not get the cells that they want to see from the fibrin. Your cells are going to be in the button, or the so called gunk on the bottom of the conical tube. You should centrifuge at least a good 50ml for a cell block. Now, if your button after centrifugation is still rather small and you have additional fluid then pour off the top and spin down more fluid until your button is a good size. G Weigel, HT (ASCP) _ Suspicious message? There’s an alert for that. http://windowslive.com/Explore/hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_broad2_122008___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
