RE: [Histonet] Reusing specimen containers
We did it like Gary wrote: We had an extra dish washer for the containers, that produce a nice melange of formalin, damp and desinfectant. Cross contamination did not occur by washing the containers but at the point of gross section or even at the OR itself Frauke Zitat von Martin, Gary gmar...@marshallmedical.org: We cleaned bottles everyday for years and never had a problem ... having said that ... it not a good idea in the end because of the time and the exposure to the formalin. Gary -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniel Schneider Sent: Tuesday, April 21, 2009 11:53 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Reusing specimen containers I have no idea what CAP or JCAHO says about this but it's a very bad idea. Cross contamination happens. If you reuse containers -- and I don't care what precautions you take -- you will rarely or occasionally fail to adequately clean a container and you will end up mixing patient tissues. The cost of a specimen container is a minute fraction of the cost of processing a specimen. Ask yourself how much money you're really saving. You're scrapping for pennies. Or is this some sort of green initiative? In any case, it's asking for problems Daniel Schneider On Tue, Apr 21, 2009 at 11:43 AM, Kelly Colpitts kelly_colpi...@hotmail.com wrote: Hi Histoland! I'm just wondering what folks out there are doing about specimen containers. Is anyone cleaning them out and reusing them? Is there any CAP or Joint Commission regulations that say that all specimen containers can only be used once or can you reuse them as long as they have been thoroughly cleaned? Thanks for you all your input, Kelly _ Windows Live(tm) SkyDrive(tm): Get 25 GB of free online storage. http://windowslive.com/online/skydrive?ocid=TXT_TAGLM_WL_skydrive_042009 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] haemotol autostainer?
A colleague is looking to purchase a staining machine, primarily for Romanowsky type staining, any recommendations or brick bats please. Richard Edwards Leicester University...UK. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] tissue non specific alkaline phosphatase antibodies
Hi, I want to find an alkaline phosphatase antibody that will label bone in sections of chick embryonic tissue. Does anyone know if such an antibody exists? Many thanks for your help Imelda Dr Imelda McGonnell Lecturer Dept. Veterinary Basic Sciences Royal Veterinary College Royal College St London NW1 0TU Phone : Extn. 5453 Direct dial 020 7468 1223 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mohs Histology position!!!
Hello Histo-land! I have a temporary Mohs Histology position available at this time. Dayshift, Monday through Friday. Please let me know if you are interested or know someone seeking a temporary position at this time. Thanks!!! :) April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asac...@titanmed.com see us on the web at www.titanmed.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CAP question
Hi all, I am updating procedures and am wondering if there is a better format for compliance with ANP.22250 - Does the procedure manual address all methods and antibodies currently in use? Just wondering if I need to include every antibody or a generic procedure covering most antibodies, with the exceptions only reference within the same procedure? Any help would be appreciated. Thanks, Mary Abosso ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CAP question
Mary - I have one generic protocol with a spreadsheet attached stating the following: Name of Antibody CD/CK # Clone Species Pretreatment Dilution Incubation time Enzyme (ABC or AP) Lot # in Use Hope this helps out it has passed in the past. take care. amy Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: port...@msu.edu Web: www.humanpathology.msu.edu - Original Message - From: Mary Abosso mabo...@unipathllc.com To: Histonet histonet@lists.utsouthwestern.edu Sent: Wednesday, April 22, 2009 1:30 PM Subject: [Histonet] CAP question Hi all, I am updating procedures and am wondering if there is a better format for compliance with ANP.22250 - Does the procedure manual address all methods and antibodies currently in use? Just wondering if I need to include every antibody or a generic procedure covering most antibodies, with the exceptions only reference within the same procedure? Any help would be appreciated. Thanks, Mary Abosso ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CAP question
I used to have a generic protocol for each type of procedure. For example: the lectin procedure for Ulex europaeus was summarized apart for the general used with most of the Abs using HIER. There was also a general protocol when the procedure was completed manually of with the Dako autostainer that we used.The procedure for Her2-neu was also apart. Also I had a spread sheet for the different antibodies in use and for each it was stated the HIER pH required and other relevant data for the antigen, as well as the type of control used. René J. --- On Wed, 4/22/09, Mary Abosso mabo...@unipathllc.com wrote: From: Mary Abosso mabo...@unipathllc.com Subject: [Histonet] CAP question To: Histonet histonet@lists.utsouthwestern.edu Date: Wednesday, April 22, 2009, 1:30 PM Hi all, I am updating procedures and am wondering if there is a better format for compliance with ANP.22250 - Does the procedure manual address all methods and antibodies currently in use? Just wondering if I need to include every antibody or a generic procedure covering most antibodies, with the exceptions only reference within the same procedure? Any help would be appreciated. Thanks, Mary Abosso ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Myeloperoxidase Antibody
Good Afternoon Everyone, Where do you recommend ordering the pre-dilute antibody Myeloperoxidase MPO Ab-2? Biocare and Lab Vision have both discontinued carrying it. Please help! Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Myeloperoxidase Antibody
I don't know if I wholeheartedly recommend it but we switched to Cell Marque. The reason for my qualifying statement is because we went from not needing any HIER to having to use a pretty harsh retrieval. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Smith Wanda Sent: Wednesday, April 22, 2009 2:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Myeloperoxidase Antibody Good Afternoon Everyone, Where do you recommend ordering the pre-dilute antibody Myeloperoxidase MPO Ab-2? Biocare and Lab Vision have both discontinued carrying it. Please help! Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] levamisole
hi everyone, can anyone tell me how to prepare 125mM of levamisole. Dr. Anjan Kumar.K.R M.V.Sc Scholar Dept. of Veterinary Pathology Madras Veterinary College Chennai-7 India email: drvet_an...@hotmail.com Phone: +91-9940475801 _ How fun is this? IMing with Windows Live Messenger just got better. http://www.microsoft.com/india/windows/windowslive/messenger.aspx___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Replacement glass plates for Reichert Microtome Knife Sharpener 903?
Yvan Unfortunetly we don't have any plates that will fit the AO903 and the AO935 knife sharpener. The SP9000 plates can only be used on the SP9000. Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailh...@leica-microsystems.com www.leica-microsystems.com yvan lindekens yvan_lindek...@y ahoo.com To Sent by: histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Replacement glass plates for Reichert Microtome Knife 04/21/2009 05:28 Sharpener 903? AM Hi all, It struck me that all those microtome knife sharpeners (AO 935, Reichert 903, AO 903...) all look very much the same. Are the glass plates interchangeable? Would the glass plates for the current Leica sharpener SP9000 (part.no. 14041819698, dimensions 292mm x 127mm x 6 mm) fit the Reichert Microtome Knife Sharpener 903? Thanks in advance for your answers! Yvan. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Finding iron in decalcified sections
Hi folks - I'd very much appreciate some help with this problem. I've been asked to performing the routine histo for a trial investigating the ability of chickens to sense direction. They are believed to have areas of iron rich tissue in their beaks which can detect the magnetic field of the earth in the same fashion as homing pigeons. The problem is this - to section the beaks I need to decalcify them, however I'm pretty sure that standard decalcification removes iron, at least partly. I know that the levels of iron in the tissue are very low and I've just trialled my technique on a pigeon with no success. I'm using a standard Perl stain (2% HCl + 2% K Ferrocyanide for 20 mins) on FFPE tissue which usually works very well for me, fixation is for a minimum of 24 hours in either 10%NBF or Bouins and decalcification is in either 5% Nitric acid or formic acid. Would anyone have any suggestions as to how to decal without removing iron or another stain which I could use in this situation? Thanks in advance for any and all suggestions, Craig Craig (Joe) Farish Senior Technical Officer Veterinary Diagnostics School of Animal and Veterinary Sciences Charles Sturt University Wagga Wagga, Australia c far...@csu.edu.au ' I don't want to achieve immortality through my work, I want to achieve immortality through not dying' - Woody Allen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
