[Histonet] MMA and benzoyl peroxide
Hi, I am doing MMA plastic embedding and have a problem with getting a cloudy embedded sample. I am using MMA plus perkadox 16. Not too sure whether the cloudiness is caused by perkadox 16. I would actually like to try to do the embedding using MMA and benzoyl peroxide. I would appreciate if anyone can share with me what kind of benzoyl peroxide and the working concentration that I should use. With the MMA and perkadox sample, I let the sample polymerize at room temperature. If I change to benzoyl peroxide, what kind of condition is the best for it to get polymerized? Thank you very much! Regards, Ooi _ Confidential information. Unauthorized use or disclosure prohibited. Refer http://www.singhealth.com.sg/ContactUs/#Disclaimer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ER labelling of fixed cells
Hi all, I'm trying to do some double labelling to show that a protein resides in the ER but I'm having some trouble getting nice reticulated ER staining in fixed cells. So far I have tried fixing in 4% PFA (15min on ice) followed by permeabilization with Tritonx100 (0.25%; 10min; room temp) or Saponin (0.5%; 7min; room temp; all susequent solutions containing 0.05% saponin). ER antibody is rabbit anti Calnexin (Stressgen). The labelling I'm getting is quite strong but is very punctate. Staining of my protein of interest is also very puntate but doesn't appear to colocalise with the clanexin and also I don't see any staining of the plasm membrane - which I should! Anyway the Prof wants to see nice reticulated staining of the ER - which I have seen in live cells with ER tracker but is it possible to get the same thing with fixed cells? I thought about trying to fix with glutaraldehyde but ?concentration ?time.. Also my PFA was in PBS would it be better to put it in HEPES or PIPES? Any suggestions welcome! Thanks Sonya ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology job Opening, Colorado
Hello Histo-Netters, We have an HT job opening here in Longmont Colorado, located north of Denver and just NE of Boulder. Please apply through the website. I have attached the link. All new and experianced HT's please apply. Also please add my name to your application so we can track the responces from histo net. http://luh.jobscience.com/JsrApp/index.cfm?cmd=showPositionDetailnextEvent=doSearchPositionscobrandId=9000masterId=luh001accountId=9F3D0B3C-E71A-17E7-69B649D49CA1C88FpositionId=511568urlArgList=c2VhcmNoVHlwZT1xdWljayZzdGFydD0xJmNvdW50PTUwJmRlcHRJZD0mam9iSWQ9JmtleXdvcmQ9bid=1297 If the link does not work the web site is www.luhcares.org Thanks Matt Lunetta BS, HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Core unit support
Hello Everybody, I was wondering what type of institutional support y'all have at your various institutions. I work for a research foundation and they want to drop my salary and have the researchers grants pay my salary and almost everything else. I am a fee-for-service lab and the CEO wants to see $25K in recharges between now and December. We offer TEM, confocal, histology and cryosectioning. We will be adding micro-CT and a deconvolution microscope that does FRET and can handle samples that have been transfected(?) with viral vectors. Is she being unrealistic? I am only 80% time and the only person down here. Should I start looking for a new position before she shuts me down? How many people have had their facilities closed by the people who don't see the big picture? Please let me know your experiences. Thanks, Paula :-} Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Support for core units
Hello Everybody, I was wondering what type of institutional support y'all have at your various institutions. I work for a research foundation and they want to drop my salary and have the researchers grants pay my salary and almost everything else. I am a fee-for-service lab and the CEO wants to see $25K in recharges between now and December. We offer TEM, confocal, histology and cryosectioning. We will be adding micro-CT and a deconvolution microscope that does FRET and can handle samples that have been transfected(?) with viral vectors. Is she being unrealistic? I am only 80% time and the only person down here. Should I start looking for a new position before she shuts me down? How many people have had their facilities closed by the people who don't see the big picture? Please let me know your experiences. Thanks, Paula :-} Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] metal embedding base molds
Hi- I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All I'm able to find at this point are the metal molds with the 'wings' on them. Does anyone know a source? With all the mergers etc it's been a little challenging. Thanks in advance Vikki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] metal embedding base molds
Try EM Sciences the distribute alot of the unique old type histology supplies. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: port...@msu.edu Web: www.humanpathology.msu.edu - Original Message - From: Victoria Baker bakevicto...@gmail.com To: histonet histo...@pathology.swmed.edu Sent: Thursday, August 06, 2009 1:13 PM Subject: [Histonet] metal embedding base molds Hi- I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All I'm able to find at this point are the metal molds with the 'wings' on them. Does anyone know a source? With all the mergers etc it's been a little challenging. Thanks in advance Vikki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mycoplasma bovis
My boss has thought up a new project for me so I need to know what I need to know about Mycoplasma bovis for IHC. He wants me to cost out the procedure. I normally do only TWO IHCs - CWD and BVD - so I'll be charting new mental territory with this one. Can anyone guide me on where to start figuring this out? Where do I get antibody? Is there a pre-existing protocol for the Ventana NexES? I don't believe in re-inventing the wheel if I don't need to. Thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Mycoplasma bovis
Hi there, Check out this database. It contains a contact list for the labs that are currently doing the Myco bovis IHC. http://ihc.sdstate.org/ -J Jenee S. Odani, D.V.M., Dipl. ACVP Veterinary Medical Officer Hawaii State Veterinary Laboratory/DAI 99-941 Halawa Valley Street, Aiea, HI, 96701 Phone: (808) 483-7131/Fax: (808) 483-7110 Breeden, Sara sbree...@nmda.nmsu.edu Sent by: histonet-boun...@lists.utsouthwestern.edu 08/06/2009 08:12 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Mycoplasma bovis My boss has thought up a new project for me so I need to know what I need to know about Mycoplasma bovis for IHC. He wants me to cost out the procedure. I normally do only TWO IHCs - CWD and BVD - so I'll be charting new mental territory with this one. Can anyone guide me on where to start figuring this out? Where do I get antibody? Is there a pre-existing protocol for the Ventana NexES? I don't believe in re-inventing the wheel if I don't need to. Thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] metal embedding base molds
Dear Vikki, Sakura still carries the metal base molds that are used with Embedding Rings. They do not have the wings that the ones for use with the Unicassettes have. Their Sakura part numbers begin from 4121 and higher. If you use the electronic catalog on Sakura's website (www.sakuraus.com) and search by product description, just type in base molds. Then click on part # 4121 and the wingless ones will be pictured. Kind regards, Nancy Klemme, Sakura Educational Services -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Thursday, August 06, 2009 10:13 AM To: histonet Subject: [Histonet] metal embedding base molds Hi- I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All I'm able to find at this point are the metal molds with the 'wings' on them. Does anyone know a source? With all the mergers etc it's been a little challenging. Thanks in advance Vikki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 69, Issue 6
Does anyone out in histology have any documentation from CMS, ASCP, or NSH that breaks down the job duties or areas for what is covered under Tech and professional part of the CPT billing codes. For example is Grossing part of the a profee or tech part of the bill. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnh...@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] What do you use for decolorizing after Hematox.
Hi all, In my HE staining I always used 1% acid alcohol. (Working with conc. HCl is not fun!) There is the Orth's solution available already made, but it is 3%. Do you use that? How do you use it? What do you use in your regulat HE stain? I am using it on an old Jung AUTOSTAINER. Michelle Research Specialist USC Keck School of Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cement in bone specimen
Peggy, If the cement you mention is or similar to PMMA, then you will not be able to cut thin sections using the sliding/sledge microtome. If I was you, I would not decal it at all as it will only make the bone less dense than the cement and will definitely waste your time if you embed it in paraffin to try and cut later. Your best and only chance at success here is to embed undemineralized in resin and cut thick or ground sections that can be polished to a desired thickness. If you would like to discuss this further, please give me a call at 317-281-1975. Best, Jack Ratliff Date: Thu, 6 Aug 2009 15:43:16 -0400 From: mdica...@kaleidahealth.org To: histo...@pathology.swmed.edu CC: Subject: [Histonet] cement in bone specimen Histonetters, We sliced a humerus bone on a band saw which was filled with cement from a previous surgery and my boss would like me to try to cut it on a sliding/sledge microtome. (The knife remains stationary) Is this possible to do? Presently, it is fixing and then I will decal it but I'm wondering if I'll be wasting my time and effort. I would appreciate anyone's experience with this. Thank you. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 2009 Best Places to Work Winner Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ist...@kaleidahealth.org or call (716) 859-. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fetal demise placenta
Maria asks about fetal demise placenta: We gross in all fetal demise placenta (usually four blocks).If the clinicians desire genetics, they take?samples from the fetus and the placenta up on the floor. (There is only a three day window for growing fibroblasts). The fetus gets a gross only treatment with weight, measurements and a photograph. If the clinicians or family desire an autopsy, whatever the gestational age, we have the parents sign an autopsy consent, as if for an adult. Regards Michael Titford USA Pathology Mobile AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
