[Histonet] Viral RNA probes
Does anyone know of a company(s) that sells/makes ISH probes to viral RNA (PR8 and or Sendai) or a company that will make custom probes. Thanks for any help!! Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Her2 fixation question
Thank you all for help with the Her 2 policy. I was really stuck writing the processing piece. I was able to finish the policy now to get our docs to pass it. THX again for the great help! Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fie...@northside.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Thursday, October 29, 2009 5:17 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Her2 fixation question I am sending this email for a friend who is having problems posting to histonet right now. Please reply to her at carol.fie...@northside.commailto:carol.fie...@northside.com and not me. From Carol Fields She writes: Hi Netters, I am attempting to write a processing schedule (VIP) for breast bx's and breast tx for Her2 fixation. We send the blocks out for testing but I cannot get my brain around this schedule for the processing piece. The lab is 24 hours with only one person from 7pm until 10 pm. We work weekends also. Would someone please let me know how they schedule the fixation and processing of these specimens. Any help with this is greatly appreciated. Thank you, Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fie...@northside.commailto:carol.fie...@northside.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cutting Paraform tissue holding devices: question.
Questions to those colleagues that are using the Paraform tissue holding devices: 1- Have you had any problems cutting these holding devices? 2- Has your cutting productivity been reduces, at least initially? 3- Have you had problems with the adhesion of the sections that also include the Paraform? Could you please share your answers with all? Thanks! René J. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] BSL-2 question
I posted the question to the ABSA Biosafety Discussion Forum. I will post replies I receive on the Histonet List. American Biological Safety Association (ABSA) http://www.absa.org ABSA - Resources http://www.absa.org/resmenu.html ABSA - Resources - E-mail Groups http://www.absa.org/resgroups.html Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.o...@fda.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Compresstome
Ed, We have a 2 year old model of the Precisionary Instrument vibrating microtome that was made specifically for fixed tissues for histology. Back then it was the VF400 model. It is supposed to be able to cut 10 micron thick sections and we have not tested that it has this capability yet. It's design is simple and it is easy to use. It's not as sexy as some of the other Vibratome units, but it seems to work pretty well. One of our researchers has a model they use for live tissue work and they have had great success with it. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] BSL-2 question
Hello Everybody out there in virus land, I am getting a room ready for BSL-2 level virus imaging. I can't get any information regarding how safe the cells are once the virus has been transfected (?) in to them. I have a BSL-2 level bio-hood, I have a room with a door and I have a brand, spanking new deconvolution with FRET and an incubator for live cell imaging. I need to know if the people in the room are safe from the virus once it's inside the cells. I can't get the air pressure changed in the room so I need to know what other precautions we might need to take. Thanks to all who can give me any pertinent information. Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] regarding validation for the instrument install and antibodies
Hello Everyone, Apart from ER/PR,howmany cases/control blocks need to be run for antibody validation? We are talking about commonly used antibodies. Thank you Nirmala Srishan Holy Name Hospital Teaneck, NJ 07666 Holy Name Hospital is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Sixth Nationally by Modern Healthcare , 2008 Best Places to Work in New Jersey, NJBIZ - 2006, 2007, 2008 2009 Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power - 2006 2007 Distinguished Hospital Awards for Clinical Excellence, HealthGrades - 2005, 2006, 2007 2008 Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades - 2008 2009 Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] job opening at UCLA
Hi all, I have an opening for a Histotech III in the histology lab which will be coming up shortly (it's not posted yet). For this position, an HTL is required, no exceptions. We are looking for someone with 5+ years of experience in routine histology, manual specials, automated special stain experience is always a help, as well as someone with leadership skills. Salary range for this position is $36.83-$47.66 per hour DOE. If you are interested, please let me know! Thanks, Noelle Noёlle Linke M.S., HTL(ASCP)QIHC Manager, Histology Services Department of Pathology Laboratory Medicine David Geffen School of Medicine at UCLA Phone: 310-825-7397 Pager: 97471 nli...@mednet.ucla.edumailto:nli...@mednet.ucla.edu IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Multi Cool Bath replacement for Histobath
Terri Bishop at SP Industries in Stone Ridge NY tells us: I wanted to take this opportunity to introduce the FTS Brand Multi Cool Bath as a replacement for the Histobath. - Please view the data sheet on our website at http://www.ftssystems.com/multicoolbath.htm The Web site doesn't clearly indicate the potential usefulness of this product in surgical pathology - for preparing specimens for frozen section. I think a lot of pathologists, Mohs surgeons, and other users of frozen section technology will be interested in the Multi Cool Bath. Can you give us any idea what it will cost? The Web site doesn't tell you what the cooling liquid is. As I've expressed a number of times (see the HistoNet archives), I thought a major problem with the Histobath was its use of acetone or 2-methylbutane (isopentane), both serious fire and explosion hazards in a pathology laboratory. Can the Multi Cool Bath be filled with a non-flammable coolant such as 3M's Novec Engineered Fluid HFE-7100,a segregated hydrofluoroether (HFE), methyl nonafluoroisobutyl ether, C4F9-O-CH3? Is this fluid compatible with the Multi Cool Bath? Note a competing product, Alan Bright's (Bright Instrument Co., in the UK) Clini-RF Rapid Freezer. I have no commercial connection with any of these companies. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Diff Quik CPT code
Actually it is 88312 (micro-organisms like Helicobacter pylori). 88313 is for non-organism special stains like iron, collagen etc. except IHC stains. Konni Black Digestive Health Laboratory Tacoma, WA - Original Message - From: Bell, Lynne lynne.b...@cvmc.org To: 'Cristi stephenson' cls71...@sbcglobal.net; histonet@lists.utsouthwestern.edu Sent: Tuesday, November 03, 2009 11:05 AM Subject: RE: [Histonet] Diff Quik CPT code The CPT code for diff-quik for H.pylori is 88313. Lynne Bell, HT (ASCP) Lead Histologist Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Schiff mystery
Peggy, I would be worried by the activated charcoal. It could be possible that the charcoal clarifying step is removing too much of the active schiff reagent. The students may be trying to obtain a clear solution and mixing the charcoal one more time. I would suggest that they stop once a yellow colour is obtained. I had a similar problem when preparing thionine-schiffs. Activated charcoal removed the active schiffs reagent as well as the contaminants so for a blue schiffs I do not clarify with charcoal. The dehydrating alcohols remove the pink-purple background staining, leaving the positive blue staining. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Monday, 2 November 2009 9:07 PM To: histonet@lists.utsouthwestern.edu Cc: 'Peggy Wenk' Subject: [Histonet] Schiff mystery Need the help of the Histonet chemistry gurus. This has happened the last 2 times the students and I made up Schiff. Two different set of students. We make up the Schiff, and use it the next day, and it works correctly (stains the glycogen in liver and neutral mucins in intestine a bright magenta). Store in refrig. 1 week later, when we go to stain fungus with PAS, the Schiff doesn't work. Try the same liver and small intestine controls. Doesn't work at all. No magenta, no pink, nothing on the slides. When going into the water rinse after Schiff, the water turns pink with the newly made Schiff, but 1 week later, there is no color change in the water rinse. When we make up the Schiff, we show the students the Schiff quality assessment, where we put a few drops of formaldehyde in it, and the Schiff changes to purple immediately. Works great newly made. One week later, when we can't get the Schiff to stain, there is no color change with formaldehyde. None. It is the same procedure we've been using for years to make up the Schiff. It is the same procedure our histology lab uses. Their Shiff lasts for months. Ours last for a few days. I think it might be one of our reagents is going bad. Histology lab has their own reagents, the School has our own. - The basic fuchsin is OK, as the Kinyoun works great. - The hydrochloric acid was taken from histology, so I'm guessing it's OK. - The charcoal is fairly new. Could that be the problem? Why? - We are using sodium metabisulfite, not sodium sulfite or sulfate. At least that's what the bottle says. Does this break down and go bad? Need all the help we can get. This is spooky, Halloween or no Halloween. Peggy Wenk, HTL(ASCP)SLS Schools of Histotechnology Beaumont Hospital Royal Oak, MI 48073 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Diff Quik CPT code
The CPT code for diff-quik for H.pylori is 88313. Lynne Bell, HT (ASCP) Lead Histologist Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Diff Quik CPT code
Hello, I was wondering what CPT code is used for performing a Diff Quik to demonstrate H. pylori? Can anyone help? Thanks, Cristi ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Peggy Wenk
I wanted to say to everyone that among all of the top folks we have out there including Peggy it is nice to know that Peggy actually uses the Histonet to GET info not only give out such great advice. In answering the question the only thing that screams out at me is technique of the students. I mean I only taught for a year at AFIP back in the 80's but over the years have had scores of interns, students, techs. I mean are they doing something before to break down the schiffs like placing in some seriously alkaline solution, water have minerals going back into the schiffs? Great question but my note is meant to compliment Peggy. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Arm Rests
Does anyone have a recommendation for microtome arm rests? Thanks in advance! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Arm Rests
Yeah - don't use em. Since I had developed tendinitis in both elbows from microtomy, EHS ergonomics people at a former employer decided to invent some. They covered them with some fake vinyl material, and the very first day I tried using them, my elbow slipped on the shiny vinyl and I cut off the tip of my finger on the blade. I have since learned that resting your forearms on the sharp edge of the bench caused my tendinitis. Since I quit that bad habit, I haven't had a recurrence in 15 years - nor have I cut myself. From: Weems, Joyce jwe...@sjha.org To: histonet@lists.utsouthwestern.edu Date: 11/03/2009 12:58 PM Subject: [Histonet] Arm Rests Sent by: histonet-boun...@lists.utsouthwestern.edu Does anyone have a recommendation for microtome arm rests? Thanks in advance! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
