Re: [Histonet] Ab Validation
We have started saving these since the NEW question came up in June of 2009. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJUSA Laurie Colbert laurie.colb...@huntingtonhospital.com 04/02/10 11:19 AM When you validate a new antibody or a new antibody lot, do you save the slides or just the paperwork (validation report from pathologist) for future inspection purposes? Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histobath
Does anyone know the normal operating temperature of the Thermo Histobath? A customer has asked me to repair theirs and I want to make sure I get the temp correct. Thanks Matt -- Matthew Mincer Tech One Biomedical Service 159 N Marion Street PMB163 Oak Park, IL 60301 office (708) 383-6040 X 10 cell(708) 822-3738 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Happy Easter!
Dear histonetters I wish to you, Happy Holidays with a lot of peace and health! dr.Mohamed Abd el razik Histology Dep. Faculty of Vet. Med. Cairo University- Egypt ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histobath
Hi Matt, Depending on the wax melting point which should be around 57 degree Celsius, your water bath should be set at 50 degree Celsius. Hope this help. Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London, WC1N 3JH UK Tel: (+44) 0207 4059200 Ext 5475 ben...@gosh.nhs.uk On Mon, Apr 5, 2010 at 1:37 PM, Matthew Mincer m...@techoneweb.com wrote: Does anyone know the normal operating temperature of the Thermo Histobath? A customer has asked me to repair theirs and I want to make sure I get the temp correct. Thanks Matt -- Matthew Mincer Tech One Biomedical Service 159 N Marion Street PMB163 Oak Park, IL 60301 office (708) 383-6040 X 10 cell(708) 822-3738 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Smile it confuses people ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histobath part 2
I forgot to mention, this Histobath I am talking about is the one with a refrigerated tank. You fill the tank with a liquid (I forget what the chemical is) and use it to quick-freeze tissue to be cut in a cryostat. Peace Matt -- Matthew Mincer Tech One Biomedical Service 159 N Marion Street PMB163 Oak Park, IL 60301 office (708) 383-6040 X 10 cell(708) 822-3738 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histobath part 2
Matt, I have a copy of the instructional/operational manual from my histobath that we purchased in 1994 from Shandon-Lipshaw. The specifications for its lowest temperature is -60C. The liquid that is used is 2-methylbutane (isopentane). I can fax you a copy if it would help. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: lpave...@hurleymc.com ph: 810-257-9948 Lab: 810-257-9138 fax: 810-762-7082 Matthew Mincer m...@techoneweb.com 4/5/2010 8:56 AM I forgot to mention, this Histobath I am talking about is the one with a refrigerated tank. You fill the tank with a liquid (I forget what the chemical is) and use it to quick-freeze tissue to be cut in a cryostat. Peace Matt -- Matthew Mincer Tech One Biomedical Service 159 N Marion Street PMB163 Oak Park, IL 60301 office (708) 383-6040 X 10 cell(708) 822-3738 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histobath
Sorry for that Matt, In this case the internal temperature of the cryostat chamber should be set at -20 to -22 degree Celsius, not sure what would the actual temperature of the histobath itself but our thermo scientific cryostat has the following settings: - the cryobar is set at -18 Degree Celsius, - the Cryo chamber at - 22 degree Celsius. Hope this help Malika Benatti Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London, WC1N 3JH UK Tel: (+44) 0207 4059200 Ext 5475 ben...@gosh.nhs.uk - Show quoted text - On Mon, Apr 5, 2010 at 1:51 PM, Matthew Mincer m...@techoneweb.com wrote: Thanks, but the Histobath I was talking about is a freezing device for cryostats. Matt Malika Benatti wrote: Hi Matt, Depending on the wax melting point which should be around 57 degree Celsius, your water bath should be set at 50 degree Celsius. Hope this help. Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London, WC1N 3JH UK Tel: (+44) 0207 4059200 Ext 5475 ben...@gosh.nhs.uk mailto:ben...@gosh.nhs.uk On Mon, Apr 5, 2010 at 1:37 PM, Matthew Mincer m...@techoneweb.commailto: m...@techoneweb.com wrote: Does anyone know the normal operating temperature of the Thermo Histobath? A customer has asked me to repair theirs and I want to make sure I get the temp correct. Thanks Matt -- Matthew Mincer Tech One Biomedical Service 159 N Marion Street PMB163 Oak Park, IL 60301 office (708) 383-6040 X 10 cell(708) 822-3738 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu mailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Smile it confuses people -- Matthew Mincer Tech One Biomedical Service 159 N Marion Street PMB163 Oak Park, IL 60301 office (708) 383-6040 X 10 cell(708) 822-3738 -- Smile it confuses people ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] 72644.18148...@web111105.mail.gq1.yahoo.com
Talk about politically incorrect. Pregnant, out of wedlock 16 year olds? Are you kidding me? Seriously AB? Jan -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, April 01, 2010 7:42 PM To: Andrew Burgeson; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] 72644.18148...@web05.mail.gq1.yahoo.com I've seen lab get accredited one month, only to have CLIA come through the next month and rip that lab apart. I have been through CAP inspections that consisted of the inspector looking only at slides while he checked off the checklist to inspectors carrying their own thermometers and testing the temp of the paraffin baths on the tissue processors. This is supposed to be a peer review, often it's a witch hunt. I had one CAP team leader tell me that my lab was the last lab he was going to inspect because he dropped out of CAP and went with JCAHO, which was approved by his hospital administrator. Does anyone know why CAP split the processing and grossing parts anyway? I thought it was a stupid idea in the first place. But then again, I've never been known to be politically correct Joe - Original Message - From: Andrew Burgeson nap...@siscom.net To: histonet@lists.utsouthwestern.edu Sent: Thursday, April 01, 2010 12:26 PM Subject: [Histonet] 72644.18148...@web05.mail.gq1.yahoo.com Sheesh is right, J. CAP is all politics as far as I am concerned. It is all about protecting the careers and paychecks of the general pathology community. I am thouroughly unimpressed with JCAHO, CAP et al. If all you need to legally run a laboratory is to be CLIA inspected, then WHY BOTHER with these subjective entities? The BS I have heard over the last few months concerning MOHS surgery specimens is one glaring example of the limitations CAP has in understanding fully certain nuances of the lab trade. Ridiculous. Unless you want the marketing and potential perception that you are better covered from a legal standpoint, CAP certs are worthless. The more I hear about CAP certifications, the more I see it as a certain community of individuals who are protecting their perceived TURF. In the end, the pathologists in the group and in the facility in which you are working have to take responsibility for these matters. If the docs think a CAP cert is necessary, then do it and live with it. If not, then consider yourself lucky to not have to see these people in your lab. I have been through MANY CAP inspections in and out of the military. For the most part, though, I see people paying this organization to inspect their lab as the same thing as burning a pinch of incense in honor of great Caesar, ruler of Rome. It will get you some kudos, but tangibly not change much at all if your pathologists or HR $ hiring hands want to pocket more $ as a result of hiring pregnant out of wedlock 16 year olds to gross tissue and cut slides. Seen it. AB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RCC
RCC from Novacastra (Leica) Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Howery, Jeffrey Sent: Friday, April 02, 2010 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RCC What are people using for a Renal cell Carcinoma. ( this is a test ) Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: CD5
High pH Retrieval for 20 minutes in the PT module 20 minute incubation of CD5 15 minute mouse linker 20 Minute incubation Flex HRP 10 minute DAB 5 minute Hematoxylin Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Howery, Jeffrey [jhowe...@yrmc.org] Sent: Friday, April 02, 2010 4:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD5 Can anyone share thier protocol for CD5. We use the Dako platform. Thanks Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Choosing Carousel Style Tissue Processors
I am in the process of selecting a new tissue processor for my lab, and have narrowed the search to two models. Both are carousel style tissue processors. We are a small, low volume research lab, so the carousel style fits well with our processing and space needs. I would appreciate any information/opinions that anyone has about these two models and manufacturers. Major lab equipment purchases seem to be on a 20 year cycle here, so I need to choose carefully and well! My choices are the Leica TP 1020 and the Thermo Scientific Microm STP 120 Spin tissue processor. Has anyone found the spin function on the Microm unit to be useful, or just another part to break down and need replacement? Thanks in advance for any information that you can offer. Cathy Parsons cpars...@vt.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: CD5
Jeff, Thermo Scientific (Formally NeoMarkers) CD5 (Clone SP19): Catalogue# RM-9119-S. Concentration of 1:75 diluted w/Dako diluent. Antigen Retrieval: PH6.0 Citrate Target Retrieval (DAKO) for 35 min. @ 99 degrees C, followed by a 20 min. room temp. cool down. 35 minute primary antibody application. 30 minute Envision+ polyclonal (DAKO) secondary antibody detection. 7 minute DAB+ (DAKO). Counter-stain as you like. Results on this antibody are excellent. Best of Luck, Glen Dawson BS, HT QIHC (ASCP) IHC Manager Milwaukee, WI From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Howery, Jeffrey [jhowe...@yrmc.org] Sent: Friday, April 02, 2010 4:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD5 Can anyone share thier protocol for CD5. We use the Dako platform. Thanks Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 72644.18148...@web111105.mail.gq1.yahoo.com
The point is not about gender, as I stated before... It's about a person's health risks and lack of training overlooked for the sake of labor. TYVM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] 72644.18148...@web111105.mail.gq1.yahoo.com
When I was 16 years-old I was grossing in the lab. We had very busy pathologists (busy reading slides) who thought it was okay to train their courier (me at the time) to gross. I had already been there handing them bottles and closing cassette lids for several months. When we had our first CLIA inspection, they had me intial and sign paperwork saying that I had grossed so many cases of various specimen types under patholgist supervision and had been grossing so long. The problem was that I was only a high school graduate at the time. They then changed direction and told the inspectors that the pathologists did all the grossing. Just brings back memories. Thought I'd share. Mark Tarango On Mon, Apr 5, 2010 at 10:03 AM, Andrew Burgeson nap...@siscom.net wrote: The point is not about gender, as I stated before... It's about a person's health risks and lack of training overlooked for the sake of labor. TYVM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Stories
To all of you who sent me your how did I get started in histology stories, I have not forgotten you! I am making all your contributions into one document and although I have about a dozen left to transcribe, if you will contact me DIRECTLY at nmhi...@comcast.net, I will send you the entire document when I finish it. Taken as a whole, it's pretty amazing/interesting/involved/riveting! Please do NOT contact me via Histonet - use my home email as above. My plan is to submit the stories to NSH to use as a tool for recruiting (it seems that most of us got our start through karma!). Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] 72644.18148...@web111105.mail.gq1.yahoo.com
I am very confuse reading every email reply to this tread also I would be really grateful if someone could enlighten with regard to what is the comment practice in the US. Having been trained as a histotechnologist although we are call Specialist Biomedical Scientist in the UK, we cannot practice unless we are fully registered with the Health Professional Council HPC, which I believe has the same role as the ASCP. Every 2 years we may be audited a demonstrate that we fully comply with HPC regulation and CPD or lose or registration. All laboratories are accredited by the Clinical Pathology Accreditation CPA under the international organization for standardization legislation (ISO 15189). Laboratory accreditation happen every 2 years cycle for which the laboratory has to comply with a set of standard. During inspection accessor review everything with a fine tooth comb, and score you some of the issues may just be minors but they will always get you with a critical issue, which you will have a set amount of time to correct, they will then return and verify that all non compliance and critical issues have been address before giving you CPA accreditation status. Having a 16 years old out of school with little experience in histology and no formal training grossing specimen is never heard off, only Register Biomedical Scientist are allowed to do small biopsies, Advance Practitioner, Trainee Pathologist, will be involved in the grossing of lager specimens, and tumour specimens. On Mon, Apr 5, 2010 at 6:18 PM, Mark Tarango marktara...@gmail.com wrote: When I was 16 years-old I was grossing in the lab. We had very busy pathologists (busy reading slides) who thought it was okay to train their courier (me at the time) to gross. I had already been there handing them bottles and closing cassette lids for several months. When we had our first CLIA inspection, they had me intial and sign paperwork saying that I had grossed so many cases of various specimen types under patholgist supervision and had been grossing so long. The problem was that I was only a high school graduate at the time. They then changed direction and told the inspectors that the pathologists did all the grossing. Just brings back memories. Thought I'd share. Mark Tarango On Mon, Apr 5, 2010 at 10:03 AM, Andrew Burgeson nap...@siscom.net wrote: The point is not about gender, as I stated before... It's about a person's health risks and lack of training overlooked for the sake of labor. TYVM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Smile it confuses people ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech Position in Bay Area is Still Open.
On Assignment Healthcare is currently looking for a Histo Tech for a contract assignment here in the Bay Area. This assignment would start ASAP and will be for an indefinite period of time as it is to covering for a leave of absence. Hours are: Monday through Friday, 5:30am to 2:00pm. For additional information please contact me via email. Erik Dokken Market Leader - Northwest Region www.oahealthcare.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Monday, April 05, 2010 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 77, Issue 5 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Re: Ab Validation (Dana Settembre) 2. Histobath (Matthew Mincer) 3. Happy Easter! (mohamed abd el razik) 4. Re: Histobath (Malika Benatti) 5. Histobath part 2 (Matthew Mincer) 6. Re: Histobath part 2 (Lynette Pavelich) 7. Re: Histobath (Malika Benatti) 8. RE: 72644.18148...@web05.mail.gq1.yahoo.com (Mahoney,Janice A) 9. RE: RCC (Bernice Frederick) 10. RE: CD5 (McMahon, Loralee A) 11. Choosing Carousel Style Tissue Processors (Parsons, Catherine) 12. RE: CD5 (Dawson, Glen) -- Message: 1 Date: Mon, 05 Apr 2010 06:30:51 -0400 From: Dana Settembre sette...@umdnj.edu Subject: Re: [Histonet] Ab Validation To: Laurie Colbert laurie.colb...@huntingtonhospital.com, histonet@lists.utsouthwestern.edu Message-ID: sbb983a4@smtpnpc.umdnj.edu Content-Type: text/plain; charset=US-ASCII We have started saving these since the NEW question came up in June of 2009. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJUSA Laurie Colbert laurie.colb...@huntingtonhospital.com 04/02/10 11:19 AM When you validate a new antibody or a new antibody lot, do you save the slides or just the paperwork (validation report from pathologist) for future inspection purposes? Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 2 Date: Mon, 05 Apr 2010 07:37:56 -0500 From: Matthew Mincer m...@techoneweb.com Subject: [Histonet] Histobath To: histonet@lists.utsouthwestern.edu Message-ID: 4bb9d9a4.6020...@techoneweb.com Content-Type: text/plain; charset=ISO-8859-1; format=flowed Does anyone know the normal operating temperature of the Thermo Histobath? A customer has asked me to repair theirs and I want to make sure I get the temp correct. Thanks Matt -- Matthew Mincer Tech One Biomedical Service 159 N Marion Street PMB163 Oak Park, IL 60301 office (708) 383-6040 X 10 cell(708) 822-3738 -- Message: 3 Date: Mon, 5 Apr 2010 05:46:25 -0700 (PDT) From: mohamed abd el razik k8...@yahoo.com Subject: [Histonet] Happy Easter! To: Histonet@lists.utsouthwestern.edu Message-ID: 794795.2296...@web112616.mail.gq1.yahoo.com Content-Type: text/plain; charset=us-ascii Dear histonetters I wish to you, Happy Holidays with a lot of peace and health! dr.Mohamed Abd el razik Histology Dep. Faculty of Vet. Med. Cairo University- Egypt -- Message: 4 Date: Mon, 5 Apr 2010 13:47:08 +0100 From: Malika Benatti malbena...@googlemail.com Subject: Re: [Histonet] Histobath To: Matthew Mincer m...@techoneweb.com Cc: histonet@lists.utsouthwestern.edu Message-ID: i2yafdd78481004050547jc7ff6082nface8b2e94908...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hi Matt, Depending on the wax melting point which should be around 57 degree Celsius, your water bath should be set at 50 degree Celsius. Hope this help. Malika Benatti BSc MIBMS Specialist Biomedical Scientist Great Ormond Street Children Hospital London, WC1N 3JH UK Tel: (+44) 0207 4059200 Ext 5475 ben...@gosh.nhs.uk On Mon, Apr 5, 2010 at 1:37 PM, Matthew Mincer m...@techoneweb.com wrote: Does anyone know the normal operating temperature of the Thermo Histobath? A customer has asked me to repair theirs and I want to make sure I get the temp correct. Thanks Matt -- Matthew Mincer Tech One Biomedical Service 159 N Marion Street PMB163 Oak Park, IL 60301 office (708) 383-6040 X 10 cell(708) 822-3738
[Histonet] Sakura E300 TP
Hi All For those of you who use or have used a Sakura E300 TP, what has your experience and overall impression been? I'm particularly interested in input from anyone who has worked with a refurbished E300, including how well it has held up (or not) compared to more recent model TPs. Any maintenance issues, malfunction problems, etc that stand out? I would be using it for a skin specimens only. Thanks very much!! Have a great day Carlos ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Amyloid Stain
Hey Guys, I was hoping to get some help with the best way to approach a request for an amyloid stain. The only thing I've ever done in the past is the traditional Congo Red... however we were wondering how the Amyloid A Immuno stain is. Our pathologists were wondering if the Amyloid A is more/less reliable and if there are any limitations associated with this antibody. I just don't know that much about it and was hoping you guys could enlighten me! Thanks for the help! Drew ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Amyloid Stain
Drew, At one time we were going to bring this stain in-house (Amyloid A) but finding positive control material was problematic. One of our pathologists remembered a case from more than 20 years ago (and had documented it!) but it was still just a tiny renal biopsy. We found that ProPath (214-237-1894, www.propath.com ) does this antibody so we gave them some of our renal biopsy and since then have sent our cases to them for staining. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew Meyer Sent: Monday, April 05, 2010 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amyloid Stain Hey Guys, I was hoping to get some help with the best way to approach a request for an amyloid stain. The only thing I've ever done in the past is the traditional Congo Red... however we were wondering how the Amyloid A Immuno stain is. Our pathologists were wondering if the Amyloid A is more/less reliable and if there are any limitations associated with this antibody. I just don't know that much about it and was hoping you guys could enlighten me! Thanks for the help! Drew ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histo position in Seattle Wa.
Med Lab Assoc. in Seattle Wa. is looking for a full time tech with experience in cutting, embedding. special stains and IHC. We are a growing lab and plan to expand into new areas of pathology/molecluar biology as well as our association with a highly regarded Clinical lab. Position is Mom-Fri., 6 AM to 2:30 PM. If interested contact me at kpie...@cancer-test.com or at 206-623-3814 between 10 AM and 5 PM Pacific daylight savings time. Ken Pierce. lab supervisor ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histo Stories, Part 2
PLEASE send requests for the histo stories to my HOME EMAIL ADDRESS - not via Histonet!!! nmhi...@comcast.net. Thank you! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] 72644.18148...@web111105.mail.gq1.yahoo.com
Comparing the situations in different countries can be very confusing. I traied in the UK (many years ago) and have lived in Canada for a long time, but I do have some (limited) information about the US system. First off, Medical Laboratory Technology in the UK and Canada includes histotechnology as one of the integral subject areas, but the US does not. Histotechnology is a standalone subject there, by and large. The ASCP is different from the Health Professional Council (used to be Council for professions supplementary to medicine when I lived there). That is a licensing body, and its function is carried out by some state agencies (in the US) and some provincial agencies (in Canada). However, not all states and provinces require licensing to work as a medical laboratory technologist/histotechnologist. The ASCP used to run the commonest US qualifying exams (still do ??, I am not sure) and kept a registry of qualified technologists, although there are others systems. In Canada it is done by the CSMLS (Canadian Society for Medical Laboratory Sciences). The equivalent organisation in the UK is the IBMS (Institute of Biomedical Sciences). In Canada it is possible to take specialty training in a subject area at both initial level and post initial level. So you can be an RT (Registered Technologist) in medical laboratory technology generally, or an RT in cytology or electron microscopy as examples. All RTs can take advanced examinations as general or specialist technologists, depending on their initial RT status. There used to be a third level (Fellowship in the CSMLS) but it was abandoned because so few technologists took it. That was about the same level as the UK three part exam. An applicable BSc is now required in Canada to advance post RT. As to 16 year olds in labs. I started work in the UK in Hackney six days before my 17th birthday in 1960. A month later I was doing haemoglobins by finger stick with Hagedorn needles, ESRs and going around the wards. A year later I was well versed in clinical chemistry (urea, glucose, bilirubin, alkaline phosphatases etc - all done manually with what passed for micro methods in those days. Students like me did about 80% of the work in those days because the profession was expanding so fast due to the introduction of the public health care system in Britain. Things change, and that just would not be allowed today. I suspect that 16 year olds doing grossing is very unusal and in the US would likely be viewed as an invitation for the pathologists to be sued. Remember, it was April 1st! In the US the CAP (College of American Pathologists) is involved in an accreditation system with other agencies. In Canada the CAP (Canadian Association of Pathologists) is a player in some accrediatation systems, but in Canada health care is legally a provincial responsibility, so accreditation is done by provincial agencies for each province. That is also the reason licensing varies from province to province here. Our country wide qualifying system by the CSMLS is a fortunate anomoly that nobody wants to change because it works so well for us. I hope this explains a little. Bryan Llewellyn - Original Message - From: Malika Benatti malbena...@googlemail.com To: Mark Tarango marktara...@gmail.com Cc: histonet@lists.utsouthwestern.edu; Andrew Burgeson nap...@siscom.net Sent: Monday, April 05, 2010 11:03 AM Subject: Re: [Histonet] 72644.18148...@web05.mail.gq1.yahoo.com I am very confuse reading every email reply to this tread also I would be really grateful if someone could enlighten with regard to what is the comment practice in the US. Having been trained as a histotechnologist although we are call Specialist Biomedical Scientist in the UK, we cannot practice unless we are fully registered with the Health Professional Council HPC, which I believe has the same role as the ASCP. Every 2 years we may be audited a demonstrate that we fully comply with HPC regulation and CPD or lose or registration. All laboratories are accredited by the Clinical Pathology Accreditation CPA under the international organization for standardization legislation (ISO 15189). Laboratory accreditation happen every 2 years cycle for which the laboratory has to comply with a set of standard. During inspection accessor review everything with a fine tooth comb, and score you some of the issues may just be minors but they will always get you with a critical issue, which you will have a set amount of time to correct, they will then return and verify that all non compliance and critical issues have been address before giving you CPA accreditation status. Having a 16 years old out of school with little experience in histology and no formal training grossing specimen is never heard off, only Register Biomedical Scientist are allowed to do small biopsies, Advance Practitioner, Trainee Pathologist, will be involved in
Re: [Histonet] 72644.18148...@web111105.mail.gq1.yahoo.com
Bryan, Thanks for your details explanation on what look to be a very different system. Though something I am confused with an maybe you or someone else can clarify this for me. In the UK to be allow to practice as a Biomedical Scientist in any laboratory discipline, you need of have a Accredited Hons Degree, and undergo your HPC registration, Specialist status is only acquire after passing the part 2 of the HPC registration (this is pretty new ). Most of fully qualify Specialist Biomedical Scientist have also Post Graduate qualifications. Now I am reading right that not all the histotechnologist in the US are ASCP registered ? Is the ASCP registration not a Mendatory requirement to practice as a histotechnologist ? Does the registration rules vary from states to states? Cheers Malika On Mon, Apr 5, 2010 at 8:37 PM, Bryan Llewellyn llewl...@shaw.ca wrote: Comparing the situations in different countries can be very confusing. I traied in the UK (many years ago) and have lived in Canada for a long time, but I do have some (limited) information about the US system. First off, Medical Laboratory Technology in the UK and Canada includes histotechnology as one of the integral subject areas, but the US does not. Histotechnology is a standalone subject there, by and large. The ASCP is different from the Health Professional Council (used to be Council for professions supplementary to medicine when I lived there). That is a licensing body, and its function is carried out by some state agencies (in the US) and some provincial agencies (in Canada). However, not all states and provinces require licensing to work as a medical laboratory technologist/histotechnologist. The ASCP used to run the commonest US qualifying exams (still do ??, I am not sure) and kept a registry of qualified technologists, although there are others systems. In Canada it is done by the CSMLS (Canadian Society for Medical Laboratory Sciences). The equivalent organisation in the UK is the IBMS (Institute of Biomedical Sciences). In Canada it is possible to take specialty training in a subject area at both initial level and post initial level. So you can be an RT (Registered Technologist) in medical laboratory technology generally, or an RT in cytology or electron microscopy as examples. All RTs can take advanced examinations as general or specialist technologists, depending on their initial RT status. There used to be a third level (Fellowship in the CSMLS) but it was abandoned because so few technologists took it. That was about the same level as the UK three part exam. An applicable BSc is now required in Canada to advance post RT. As to 16 year olds in labs. I started work in the UK in Hackney six days before my 17th birthday in 1960. A month later I was doing haemoglobins by finger stick with Hagedorn needles, ESRs and going around the wards. A year later I was well versed in clinical chemistry (urea, glucose, bilirubin, alkaline phosphatases etc - all done manually with what passed for micro methods in those days. Students like me did about 80% of the work in those days because the profession was expanding so fast due to the introduction of the public health care system in Britain. Things change, and that just would not be allowed today. I suspect that 16 year olds doing grossing is very unusal and in the US would likely be viewed as an invitation for the pathologists to be sued. Remember, it was April 1st! In the US the CAP (College of American Pathologists) is involved in an accreditation system with other agencies. In Canada the CAP (Canadian Association of Pathologists) is a player in some accrediatation systems, but in Canada health care is legally a provincial responsibility, so accreditation is done by provincial agencies for each province. That is also the reason licensing varies from province to province here. Our country wide qualifying system by the CSMLS is a fortunate anomoly that nobody wants to change because it works so well for us. I hope this explains a little. Bryan Llewellyn - Original Message - From: Malika Benatti malbena...@googlemail.com To: Mark Tarango marktara...@gmail.com Cc: histonet@lists.utsouthwestern.edu; Andrew Burgeson nap...@siscom.net Sent: Monday, April 05, 2010 11:03 AM Subject: Re: [Histonet] 72644.18148...@web05.mail.gq1.yahoo.com I am very confuse reading every email reply to this tread also I would be really grateful if someone could enlighten with regard to what is the comment practice in the US. Having been trained as a histotechnologist although we are call Specialist Biomedical Scientist in the UK, we cannot practice unless we are fully registered with the Health Professional Council HPC, which I believe has the same role as the ASCP. Every 2 years we may be audited a demonstrate that we fully comply with HPC regulation and CPD or lose or registration. All laboratories are
Re: [Histonet] Amyloid Stain
I find IHC for amyloid A (AA) helpful only in kidney; however, it is not requested often. Richard Richard W. Cartun, Ph.D. Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Drew Meyer 41dm...@gmail.com 4/5/2010 2:37 PM Hey Guys, I was hoping to get some help with the best way to approach a request for an amyloid stain. The only thing I've ever done in the past is the traditional Congo Red... however we were wondering how the Amyloid A Immuno stain is. Our pathologists were wondering if the Amyloid A is more/less reliable and if there are any limitations associated with this antibody. I just don't know that much about it and was hoping you guys could enlighten me! Thanks for the help! Drew ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
http://fulavaxeg.ucoz.ua/ _ Hotmail is redefining busy with tools for the New Busy. Get more from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_2___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD34 marker for Rat tisue
Does anyone have a protocol or any information on CD34 antibody that works on FFPE rat tissue? Thank you Dusko Trajkovic ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] iNOS/nitrotyrosine antibody
Dear All Looking for the best possible iNOS and nitrotyrosine antibodies that work with rat brain tissue on paraffin section I used the one from chemicon in the past but results were not so great I also use the one from labvision with disappointing results. Any help ? Thanks Dr Fabrice GANKAM -Message d'origine- De : histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] De la part de Bryan Llewellyn Envoyé : lundi 5 avril 2010 21:37 À : Histonet; malbena...@gmail.com Objet : Re: [Histonet] 72644.18148...@web05.mail.gq1.yahoo.com Comparing the situations in different countries can be very confusing. I traied in the UK (many years ago) and have lived in Canada for a long time, but I do have some (limited) information about the US system. First off, Medical Laboratory Technology in the UK and Canada includes histotechnology as one of the integral subject areas, but the US does not. Histotechnology is a standalone subject there, by and large. The ASCP is different from the Health Professional Council (used to be Council for professions supplementary to medicine when I lived there). That is a licensing body, and its function is carried out by some state agencies (in the US) and some provincial agencies (in Canada). However, not all states and provinces require licensing to work as a medical laboratory technologist/histotechnologist. The ASCP used to run the commonest US qualifying exams (still do ??, I am not sure) and kept a registry of qualified technologists, although there are others systems. In Canada it is done by the CSMLS (Canadian Society for Medical Laboratory Sciences). The equivalent organisation in the UK is the IBMS (Institute of Biomedical Sciences). In Canada it is possible to take specialty training in a subject area at both initial level and post initial level. So you can be an RT (Registered Technologist) in medical laboratory technology generally, or an RT in cytology or electron microscopy as examples. All RTs can take advanced examinations as general or specialist technologists, depending on their initial RT status. There used to be a third level (Fellowship in the CSMLS) but it was abandoned because so few technologists took it. That was about the same level as the UK three part exam. An applicable BSc is now required in Canada to advance post RT. As to 16 year olds in labs. I started work in the UK in Hackney six days before my 17th birthday in 1960. A month later I was doing haemoglobins by finger stick with Hagedorn needles, ESRs and going around the wards. A year later I was well versed in clinical chemistry (urea, glucose, bilirubin, alkaline phosphatases etc - all done manually with what passed for micro methods in those days. Students like me did about 80% of the work in those days because the profession was expanding so fast due to the introduction of the public health care system in Britain. Things change, and that just would not be allowed today. I suspect that 16 year olds doing grossing is very unusal and in the US would likely be viewed as an invitation for the pathologists to be sued. Remember, it was April 1st! In the US the CAP (College of American Pathologists) is involved in an accreditation system with other agencies. In Canada the CAP (Canadian Association of Pathologists) is a player in some accrediatation systems, but in Canada health care is legally a provincial responsibility, so accreditation is done by provincial agencies for each province. That is also the reason licensing varies from province to province here. Our country wide qualifying system by the CSMLS is a fortunate anomoly that nobody wants to change because it works so well for us. I hope this explains a little. Bryan Llewellyn - Original Message - From: Malika Benatti malbena...@googlemail.com To: Mark Tarango marktara...@gmail.com Cc: histonet@lists.utsouthwestern.edu; Andrew Burgeson nap...@siscom.net Sent: Monday, April 05, 2010 11:03 AM Subject: Re: [Histonet] 72644.18148...@web05.mail.gq1.yahoo.com I am very confuse reading every email reply to this tread also I would be really grateful if someone could enlighten with regard to what is the comment practice in the US. Having been trained as a histotechnologist although we are call Specialist Biomedical Scientist in the UK, we cannot practice unless we are fully registered with the Health Professional Council HPC, which I believe has the same role as the ASCP. Every 2 years we may be audited a demonstrate that we fully comply with HPC regulation and CPD or lose or registration. All laboratories are accredited by the Clinical Pathology Accreditation CPA under the international organization for standardization legislation (ISO 15189). Laboratory accreditation happen every 2 years cycle for which the laboratory has to comply with a set of standard. During inspection accessor review everything
RE: [Histonet] Removal of carbon pigment
Muhammad, Unfortunately you are stuck with the carbon. No technique (short of removing the cells/tissue section) will remove the carbon. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of tahs...@brain.net.pk Sent: Saturday, 3 April 2010 3:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Removal of carbon pigment Hellow all. We have received CT guided FANA smears from lung .The siles are full of carbon particles that mask the under histiocytes and granulomata.W require the removal of carbor particles.Kindly help out to remove them. Muhammad Tahseen Supervisor Histology Javed uz zaman Supervisor Cytology SKMT PAKISTAN LAHORE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Amyloid Stain
I'd interpret a request for an amyloid stain as just that - rather than immunohistochemistry. Congo red is of course the traditional dye for staining amyloid. It's long been out of use in the textile industry, and apparently is manufactured in small quantities for histologic use, and is certified by the Biological Stain Commission as an amyloid stain. I'm not certain it's still in manufacture. Holde Puchtler introduced Sirius red as a substitute for Congo red in the 1970's, and it still has some vogue. Once again it may not be in manufacture. Dick Dapson at Anatech (www.anatechltdusa.com - I have no connection with them) introduced Amyloid Red (Direct red 72, C.I. 29200) as an amyloid stain. He describes it as rather similar to Congo red. I haven't seen it - has anyone? You can get it from Anatech. Examination of a dye stain for amyloid requires a polarization system (and no, I don't mean a pair of broken sun glasses in the desk drawer), and if the pathologist doesn't have access to a polarizer (many of us don't) you probably ought to be sending out your amyloid stains. Finally, control material is hard to get. (The best controls I've seen in recent years have been sections of medullary thyroid carcinomas.) Supposedly undeparaffinized sections don't keep for more than a month, so that the control block may need to be recut for each occasional use, obviously an unthrifty procedure. I've asked this question several times over the years and never got an answer. It's fairly easy to produce amyloidosis in laboratory animals. Why isn't animal material available for controls? Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cleaning VIP Processor containers
Hi Brandi Do you do hot water flushes? If not the film will be a build up of the buffer salts from your formalin. To flush replace your formalin containers with hot water and write a program to run each container for 10-15 minutes. This will not only clean the retorts but the fluid lines as well. This should be done weekly or at least monthly if time is an issue. regards Tony Tony Reilly B.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_rei...@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
