RE: [Histonet] IgG and IgM on mouse kidneys
Liz, You can perform IgG and IgM on FFPE tissue samples. We do them through IHC on our Ventana Ultra and get the antibodies from Cell Marque. Our pathologists are happy with them. Sheila -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Saturday, February 19, 2011 5:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IgG and IgM on mouse kidneys Hello all I have a quick question. Can I perform IgG and IgM staining on mouse kidneys on FFPE tissue samples? I thought that this type of staining is normally completed on frozen sections via Immunofluorescence. Any advice would be helpful Thanks Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CAP
Lot's of Labs in LA are in their CAP window! We had our CAP inspection yesterday and having our summation this morning at 9:00. I think our department did pretty good. Keeping my fingers crossed. Akemi Allison BS, HT(ASCP)HTL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sirius red stain - picric acid substitute - water -really?
I would do a trial run on extra slides or non-valuable tissue? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mia Woodruff Sent: Tuesday, February 22, 2011 10:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sirius red stain - picric acid substitute - water -really? Hello all, I have been undertaking sirius red staining using picric acid, I was under the impression (from papers) that picric acid is an important component of the procedure and read that it prevents non-specific binding of the dye to things other than collagen. However, I have recently found a paper which suggests I can simply use water instead of picric acid, seems quite a long shot but I wondered if anyone has experience with the water technique- given that picric acid is pretty dangerous I would be keen to move away from using it but only if scientifically sound to do so. I don't want to jeopardize my results but if this method works then it's a far safer and cheaper safer approach. Any advice? Paper: A specific quantitative assay for collagen synthesis by cells seeded in collagen-based biomaterials using sirius red F3B precipitation LEE D.A.; ASSOKU E.; DOYLE V. Journal of Materials Science: Materials in Medicinehttp://www.ingentaconnect.com/content/klu/jmsm, Volume 9, Number 1, 1998 , pp. 47-51(5) Many thanks Mia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CAP
Akemi, Keep us informed!!! I too, am in my CAP window!!! Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Wednesday, February 23, 2011 9:03 AM To: histonet Subject: [Histonet] CAP Lot's of Labs in LA are in their CAP window! We had our CAP inspection yesterday and having our summation this morning at 9:00. I think our department did pretty good. Keeping my fingers crossed. Akemi Allison BS, HT(ASCP)HTL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sirius red stain - picric acid substitute - water -really?
Since you can buy a saturated Picric acid solution and I have for years why are you worried? It is stable in a saturated liquid and you can purchase fairly small amounts at a time. I only worry if find some in a powder form turning a nasty colour. It has been about twenty five years since I had that happen and immediately went to pre-mixed solutions. Pam Marcum UAMS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Wednesday, February 23, 2011 8:04 AM To: Mia Woodruff; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sirius red stain - picric acid substitute - water -really? I would do a trial run on extra slides or non-valuable tissue? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mia Woodruff Sent: Tuesday, February 22, 2011 10:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sirius red stain - picric acid substitute - water -really? Hello all, I have been undertaking sirius red staining using picric acid, I was under the impression (from papers) that picric acid is an important component of the procedure and read that it prevents non-specific binding of the dye to things other than collagen. However, I have recently found a paper which suggests I can simply use water instead of picric acid, seems quite a long shot but I wondered if anyone has experience with the water technique- given that picric acid is pretty dangerous I would be keen to move away from using it but only if scientifically sound to do so. I don't want to jeopardize my results but if this method works then it's a far safer and cheaper safer approach. Any advice? Paper: A specific quantitative assay for collagen synthesis by cells seeded in collagen-based biomaterials using sirius red F3B precipitation LEE D.A.; ASSOKU E.; DOYLE V. Journal of Materials Science: Materials in Medicinehttp://www.ingentaconnect.com/content/klu/jmsm, Volume 9, Number 1, 1998 , pp. 47-51(5) Many thanks Mia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CAP
Akemi, were you inspected with the new CAP checklist or the old one? The situation in our lab is that we were given the checklist and then they changed us to the new format. Your thoughts -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Wednesday, February 23, 2011 7:03 AM To: histonet Subject: [Histonet] CAP Lot's of Labs in LA are in their CAP window! We had our CAP inspection yesterday and having our summation this morning at 9:00. I think our department did pretty good. Keeping my fingers crossed. Akemi Allison BS, HT(ASCP)HTL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CAP
We just had ours last week. We did well, but the inspecting Pathologist said the lab smelled like oranges we were in a way too cramped space. Go figure.. Akemi Allison akemiat3...@yahoo.com 2/23/2011 9:02 AM Lot's of Labs in LA are in their CAP window! We had our CAP inspection yesterday and having our summation this morning at 9:00. I think our department did pretty good. Keeping my fingers crossed. Akemi Allison BS, HT(ASCP)HTL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CAP
Sometimes it is a good thing when CAP says you are cramped for space. It sometimes makes the hospital Administration wake up and take notice!!! W WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, February 23, 2011 10:29 AM To: histonet; Akemi Allison Subject: Re: [Histonet] CAP We just had ours last week. We did well, but the inspecting Pathologist said the lab smelled like oranges we were in a way too cramped space. Go figure.. Akemi Allison akemiat3...@yahoo.com 2/23/2011 9:02 AM Lot's of Labs in LA are in their CAP window! We had our CAP inspection yesterday and having our summation this morning at 9:00. I think our department did pretty good. Keeping my fingers crossed. Akemi Allison BS, HT(ASCP)HTL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CAP
What?!? A histology lab with no enough space...who ever heard of such a thing Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, February 23, 2011 9:29 AM To: histonet; Akemi Allison Subject: Re: [Histonet] CAP We just had ours last week. We did well, but the inspecting Pathologist said the lab smelled like oranges we were in a way too cramped space. Go figure.. Akemi Allison akemiat3...@yahoo.com 2/23/2011 9:02 AM Lot's of Labs in LA are in their CAP window! We had our CAP inspection yesterday and having our summation this morning at 9:00. I think our department did pretty good. Keeping my fingers crossed. Akemi Allison BS, HT(ASCP)HTL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AMACR (P504S)
Hi Everyone, Our pathologists are interested in using this antibody to detect metastatic renal cell carcinoma in paraffin sections. They are not interested in an antibody cocktail, but also want to use it to detect prostate cancer. I'd like to know what company and clone most labs are using. Thanks! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: [IHCRG] AMACR (P504S)
We currently use Cat#Z2001, clone 13H4 from Zeta Corp with great results, although we only use it for HMWCK/p63/P504s stain. We have also used a predilute (PP200) from BioCare (that worked quite well). Sally From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of Taylor, Jean Sent: Wednesday, February 23, 2011 9:41 AM To: histonet@lists.utsouthwestern.edu; ih...@googlegroups.com Subject: [IHCRG] AMACR (P504S) Hi Everyone, Our pathologists are interested in using this antibody to detect metastatic renal cell carcinoma in paraffin sections. They are not interested in an antibody cocktail, but also want to use it to detect prostate cancer. I'd like to know what company and clone most labs are using. Thanks! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -- You received this message because you are subscribed to the Google Groups ihcrg group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ih...@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscr...@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: hi...@nsh.org or call 443.535.4060. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CAP
That's a good thing! That means you need more ventilation and admin listens when inspectors speak! Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net Angela Bitting akbitt...@geisinger.edu Sent by: histonet-boun...@lists.utsouthwestern.edu 02/23/2011 10:30 AM To histonet histonet@lists.utsouthwestern.edu, Akemi Allison akemiat3...@yahoo.com cc Subject Re: [Histonet] CAP We just had ours last week. We did well, but the inspecting Pathologist said the lab smelled like oranges we were in a way too cramped space. Go figure.. Akemi Allison akemiat3...@yahoo.com 2/23/2011 9:02 AM Lot's of Labs in LA are in their CAP window! We had our CAP inspection yesterday and having our summation this morning at 9:00. I think our department did pretty good. Keeping my fingers crossed. Akemi Allison BS, HT(ASCP)HTL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Digital Pathology/Telepathology
Andrew We are a research contract lab and have been scanning slides (WSI) since 2007. We have an Aperio Scanscope XT. Our clients have secure remote access to their images. We manage our own server, storage (20TB) and backup locally. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrew Byrnes Sent: Wednesday, February 23, 2011 6:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Digital Pathology/Telepathology Dear Histonet, Please don't take this as spam. I am just gathering some information... I am curious to know if any of you are currently working with digital whole slide scanners and what your experience has been to date. Additionally, my company provides telepathology services by putting a digital scanner into your lab allowing our pathology group to access electronic requisitions and digital slides remotely. We then return a report electronically within 24 hours. All is stored on our servers for secure access from anywhere at anytime. The idea is to allow easy access to our fully sub-specialized pathology group by any laboratories that may have a need for more consistent pathology coverage or if there are quality issues. Same concept as teleradiology (Virtual Radiologic, Nighthawk Radiology) which is implemented in many hospitals throughout the US. If interested, our website is www.accelpath.com Would love to hear your thoughts on these subjects... digital path and telepath. Have a great day! Andrew Byrnes AccelPath ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CAP
We were given the new CAP checklist. I totally revamped our SOP Manuals, ALL QC forms, etc. to comply. This is the 1st Childrens Hospital CAP inspection I have undergone. We are inspected by other Children Hospital inspecting teams. After all the hard work, they did not go through any of our SOP's, QC manuals, Special Stains, validation protocols manuals for either histology or the IHC lab. Very odd indeed. They spoke with the AP manager for our department only, except to ask one of the histology techs how we disposed of our hazardous waste. Stay tuned till after the summation. Akemi Allison BS, HT(ASCP)HTL From: Jesus Ellin jel...@yumaregional.org To: Akemi Allison akemiat3...@yahoo.com; histonet histonet@lists.utsouthwestern.edu Sent: Wed, February 23, 2011 7:23:43 AM Subject: RE: [Histonet] CAP Akemi, were you inspected with the new CAP checklist or the old one? The situation in our lab is that we were given the checklist and then they changed us to the new format. Your thoughts -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Wednesday, February 23, 2011 7:03 AM To: histonet Subject: [Histonet] CAP Lot's of Labs in LA are in their CAP window! We had our CAP inspection yesterday and having our summation this morning at 9:00. I think our department did pretty good. Keeping my fingers crossed. Akemi Allison BS, HT(ASCP)HTL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: LCM archival FFPE tissue (Margaryan, Naira)
I have used archival FFPE slides that had used antigen retrieval methods for LCM and the yields seemed to be good. The RNA/DNA yields obviously depend on how many cells you are collecting. We routinely used 8um sections for the LCM to increase yields. If you are interested in collecting a population that is very limited in your samples (i.e., ER+ or PR+ in small tumors), you will need to have maybe 20 or more slides (estimate). I would collect those slides into a single microcentrifuge tube (insert LCM cap and invert it to get cells into buffer) using lysing buffer, heat at 42C for 30 min, spin at 2000x g for 1 min, decant buffer, resuspend pellet into RLT/70% ethanol, and freeze the samples at -80 degrees C until ready for PCR. There are some excellent protocols from Quiagen online that will be helpful. Hope this helps. Donna L. Suresch Merck Research Laboratories Research Biologist Imaging Research - West Point Mail Stop: WP44KOffice: WP44-H129 770 Sumneytown Pike PO Box 4 West Point, PA 19486-0004 Phone: 215-652-7349 Fax: 215-993-6803 Suresch, Donna L..vcf Message: 4 Date: Tue, 22 Feb 2011 13:26:47 -0600 From: Margaryan, Naira nmargar...@childrensmemorial.org Subject: [Histonet] LCM archival FFPE tissue To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: c1ba93040c6b9a4a8d84878f93fec36a09a52a3...@cmhexcc01mbx.childrensmemorial.org Content-Type: text/plain; charset=utf-8 Hi all, I have a couple questions I would like to throw out to the experts: 1) 1) Is it possible to extract RNA and DNA for gene expression purpose after usual IHC procedure on 4um sections of archival FFPE breast tumor tissue (If tissue was in citrate buffer, pH 6.0; several blocking steps; one hour in primary Ab (less then one hour will not work); with immunoperoxidase-DAB detection follow the Hematoxylin? 2) Or I need only HE? 2) 3) Is 4-5 um section good enough? 3) 4) How many sections need to have really good and highest yields? 4) 5) What temperature of Proteinase K is need 42º or 60ºC? 5) I've the LCM system from Arcturus and the Pico Pure Kit. Any suggestions? Thanks in advance, Naira Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Bone marrow charges
Are your blocks (1 bx and 1 clot) separate specimens, or just one specimen with 2 blocks? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Goodwin, Diana Sent: Monday, February 21, 2011 11:40 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Bone marrow charges Greetings, Histonetters. How many times can I charge 88313 for a bone marrow case that has an Iron stain on 2 separate blocks (one for the bx and one for the aspirate clot) and also on 1 smear made from the aspirate clot, a PAS on the 2 blocks, a Trichrome and a Retic on the bx. block, and a Wright/ Giemsa on 4 smears? Thank you!!! Diana Goodwin Supervisor, Histology Laboratory RWJUHHNJ Diana Goodwin Supervisor, Histology Laboratory xt. 6996 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Diff Quik/Wright stain for stool samples
Hello All, Does anyone use a Diff Quik protocol for parasites in stool samples and if so could you send me a copy of your procedure? I would greatly appreciate it. Thank you all. Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products 407 Interchange St. | McKinney, TX 75071 Direct: 972-436-1010 x229 | Fax: 972-436-1369 dsi...@statlab.commailto:maub...@statlab.com | www.statlab.comhttp://www.statlab.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CAP
If your lab smells like oranges, just open your CAP window and air it out. Sincerely, Jay A. Lundgren M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Joint Comm and patient identifiers
Hi Histonetters! I have a question related to the two patient identifiers that TJC requires: can anyone point (online) me to the actual regulation? It was my understanding that the 2 identifiers related to the *collection* of the specimen, meaning that the container and associated requisition had to have 2 positive patient identifiers. The question is, do they *specifically* state that the 2 identifiers must be carried through to the final surgical slide? The reason I ask is that I have a friend who got dinged for their slides not having 2 patient identifiers on them. They have the surgical number and name of institution, but not the patient name or MRN. My friend is just looking for the actual statute so that he can read and follow exactly as expected. Also, can anyone confirm that the surgical number and a bar code would suffice as 2 identifiers? Thanks! Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC Equipment
Hi Histonetters I currently use a Dako stainer for my IHC staining. It is a work horse with very little problems. It is a older model that we may need to replace in the near future. What is everyone using out in histoland. I would be perfectly willing to purchase another Dako but I want to explore all avenues before making a decision. What are the pros and cons of the instruments any of you are using. How often is the machine down? What is the capacity? We run the Dako twice daily usually to the capacity of 48 slides. I would like to hear only from actual user of the instrumentation, no vendors please. This is only a fact finding e-mail. Thanks in advance for all your input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] use of destained sections in IHC?
Hi all, Is it possible to de-stain HE stained paraffin sections and re-use them for IHC staining??? Does anything special have to be done? Will it only work for some epitopes/stains? Does anyone have suggestions on how to do this? I am being asked to do this and don't want to waste my time if it won't work. I would appreciate any suggestions anyone might have Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_d...@compbio.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CAP
I did notice CAP inspectors concentrated more on safety this time around. Akemi Allison akemiat3...@yahoo.com 2/23/2011 11:50 AM We were given the new CAP checklist. I totally revamped our SOP Manuals, ALL QC forms, etc. to comply. This is the 1st Childrens Hospital CAP inspection I have undergone. We are inspected by other Children Hospital inspecting teams. After all the hard work, they did not go through any of our SOP's, QC manuals, Special Stains, validation protocols manuals for either histology or the IHC lab. Very odd indeed. They spoke with the AP manager for our department only, except to ask one of the histology techs how we disposed of our hazardous waste. Stay tuned till after the summation. Akemi Allison BS, HT(ASCP)HTL From: Jesus Ellin jel...@yumaregional.org To: Akemi Allison akemiat3...@yahoo.com; histonet histonet@lists.utsouthwestern.edu Sent: Wed, February 23, 2011 7:23:43 AM Subject: RE: [Histonet] CAP Akemi, were you inspected with the new CAP checklist or the old one? The situation in our lab is that we were given the checklist and then they changed us to the new format. Your thoughts -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Wednesday, February 23, 2011 7:03 AM To: histonet Subject: [Histonet] CAP Lot's of Labs in LA are in their CAP window! We had our CAP inspection yesterday and having our summation this morning at 9:00. I think our department did pretty good. Keeping my fingers crossed. Akemi Allison BS, HT(ASCP)HTL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Joint Comm and patient identifiers
The Joint Commission web site: http://www.jointcommission.org/accreditation/accreditation_main.aspx click on Standards and then click on National Patient Safety Goals. It is the first standard in the chapter. Standard number: NPSG.01.01.01 Debbie M. Boyd, HT(ASCP) l Chief Hitologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net histot...@imagesbyhopper.com Sent by: histonet-boun...@lists.utsouthwestern.edu 02/23/2011 02:40 PM To 'histonet' histonet@lists.utsouthwestern.edu cc Subject [Histonet] Joint Comm and patient identifiers Hi Histonetters! I have a question related to the two patient identifiers that TJC requires: can anyone point (online) me to the actual regulation? It was my understanding that the 2 identifiers related to the *collection* of the specimen, meaning that the container and associated requisition had to have 2 positive patient identifiers. The question is, do they *specifically* state that the 2 identifiers must be carried through to the final surgical slide? The reason I ask is that I have a friend who got dinged for their slides not having 2 patient identifiers on them. They have the surgical number and name of institution, but not the patient name or MRN. My friend is just looking for the actual statute so that he can read and follow exactly as expected. Also, can anyone confirm that the surgical number and a bar code would suffice as 2 identifiers? Thanks! Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] use of destained sections in IHC?
Robin, We just take the coverslip/film off, hydrate to water and run for IHC BUT many times the HE slide is not a + slide so we end up losing tissue. It is not necessary to destain the HE first. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robin Dean Sent: Wednesday, February 23, 2011 1:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] use of destained sections in IHC? Hi all, Is it possible to de-stain HE stained paraffin sections and re-use them for IHC staining??? Does anything special have to be done? Will it only work for some epitopes/stains? Does anyone have suggestions on how to do this? I am being asked to do this and don't want to waste my time if it won't work. I would appreciate any suggestions anyone might have Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_d...@compbio.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] use of destained sections in IHC?
It will work and if you are performing antigen retrieval then you won't need to decolorize the slide, just rehydrate and perform per you usual protocol. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robin Dean [robin_d...@compbio.com] Sent: Wednesday, February 23, 2011 2:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] use of destained sections in IHC? Hi all, Is it possible to de-stain HE stained paraffin sections and re-use them for IHC staining??? Does anything special have to be done? Will it only work for some epitopes/stains? Does anyone have suggestions on how to do this? I am being asked to do this and don't want to waste my time if it won't work. I would appreciate any suggestions anyone might have Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_d...@compbio.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RRAS
So...trying to find someone who sells RRAS antibody that isn't Abcam. I can't seem to make the Abcam antibody work and I have been asked to try and get it from a different company. 2. Does anyone have any experience with RRAS? I have tried dilutions from 1:500 all the way up to 1:2000. I keep getting a blush endogenous stain that I can't seem to get out even after blocking for everything under the sun. I am using a normal pH antigen retrieval (DAKO). Should I try high pH? I don't know if that will make the positive staining come out, I think it will make it worse? Thanks histo-hotties!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Short term tissue storage PBS???
Hi All, It has come to my attention recently that some of our histology clients are submitting their samples in PBS after fixation, instead of 70% alcohol. When I ask them why they don't know and say they have been told to. I have always followed the procedure that after fixation specimens are stored, short term in 70% alcohol, until you are ready to process them. Has something changed lately that I am not aware of? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 (3355) Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Short term tissue storage PBS???
Nothing has changed, it is just one of those things people do because of fundamental ignorance. PBS is not a preservative and could even favor bacterial growth (depending of the time elapsed). After proper fixation, tissues should be placed in EthOL 70% as a general practice.PRené J. --- On Wed, 2/23/11, Jo-Ann Bader, Ms. jo-ann.ba...@mcgill.ca wrote: From: Jo-Ann Bader, Ms. jo-ann.ba...@mcgill.ca Subject: [Histonet] Short term tissue storage PBS??? To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Date: Wednesday, February 23, 2011, 3:57 PM Hi All, It has come to my attention recently that some of our histology clients are submitting their samples in PBS after fixation, instead of 70% alcohol. When I ask them why they don't know and say they have been told to. I have always followed the procedure that after fixation specimens are stored, short term in 70% alcohol, until you are ready to process them. Has something changed lately that I am not aware of? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 (3355) Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Short term tissue storage PBS???
Jo-Ann, Nothing is changed, but storage in PBS does not affect neither morphology nor antigens. I used to keep some neutral formalin- or formaldehyde-PBS fixed specimens up to 1 year in sterile PBS at +4 C without any visible changes. So - don't worry. It will be fine. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: agleiber...@cbiolabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jo-Ann Bader, Ms. Sent: Wednesday, February 23, 2011 3:57 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Short term tissue storage PBS??? Hi All, It has come to my attention recently that some of our histology clients are submitting their samples in PBS after fixation, instead of 70% alcohol. When I ask them why they don't know and say they have been told to. I have always followed the procedure that after fixation specimens are stored, short term in 70% alcohol, until you are ready to process them. Has something changed lately that I am not aware of? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 (3355) Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: alcian blue-PAS control
Melissa, We use a multi-tissue control. Our control block contains appendix, umbilical cord and liver (for diastase). The optimum control (if you can get it) would be an intestinal metaplasia of the stomach and combine this with a mucin-producing mesothelioma. To you all, any further ideas? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa Sent: Wednesday, 23 February 2011 11:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] alcian blue-PAS control Hello, Can anyone recommend a good control tissue to use for Alcian blue-PAS? Melissa Kuhnla Lead Medical Technologist for IHC and FISH Catholic Health Services of Long Island Regional Laboratory Services The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] use of destained sections in IHC?
Yes, you can use HE-stained slides for IHC; however, if your lesion/tumor is negative and there is no internal positive control, then you may be dealing with a false negative result. Having said that, we have had good luck with the majority of proteins we have tested. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Robin Dean robin_d...@compbio.com 2/23/2011 2:43 PM Hi all, Is it possible to de-stain HE stained paraffin sections and re-use them for IHC staining??? Does anything special have to be done? Will it only work for some epitopes/stains? Does anyone have suggestions on how to do this? I am being asked to do this and don't want to waste my time if it won't work. I would appreciate any suggestions anyone might have Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_d...@compbio.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: alcian blue-PAS control
Cervix (with both endocervix and ectocervix) contains both glycogen (ecto) and mucin (endo). Jennifer Tony Henwood antho...@chw.edu.au Sent by: histonet-boun...@lists.utsouthwestern.edu 02/23/2011 01:56 PM To 'Kuhnla, Melissa' melissa.kuh...@chsli.org, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu cc Subject [Histonet] RE: alcian blue-PAS control Melissa, We use a multi-tissue control. Our control block contains appendix, umbilical cord and liver (for diastase). The optimum control (if you can get it) would be an intestinal metaplasia of the stomach and combine this with a mucin-producing mesothelioma. To you all, any further ideas? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa Sent: Wednesday, 23 February 2011 11:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] alcian blue-PAS control Hello, Can anyone recommend a good control tissue to use for Alcian blue-PAS? Melissa Kuhnla Lead Medical Technologist for IHC and FISH Catholic Health Services of Long Island Regional Laboratory Services The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] use of destained sections in IHC?
I wouldn't decolorize the slides. Just remove the coverslip and hydrate to water. Any antigen retrieval should take out the Eosin. You're going to counterstain in Hematoxylin any way. Years ago (ok, many moons ago) I did an informal study. I took 10 antibodies, stained two sets of controls H E. One set, I used 1% acid-alcohol to decolorize the slides, the other set I just rehydrated to water. The slides that were placed in acid-alcohol either had no staining or a marked decrease in intensity. Joe - Original Message - From: Walter Benton wben...@cua.md To: Robin Dean robin_d...@compbio.com; histonet@lists.utsouthwestern.edu Sent: Wednesday, February 23, 2011 2:00 PM Subject: RE: [Histonet] use of destained sections in IHC? It will work and if you are performing antigen retrieval then you won't need to decolorize the slide, just rehydrate and perform per you usual protocol. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robin Dean [robin_d...@compbio.com] Sent: Wednesday, February 23, 2011 2:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] use of destained sections in IHC? Hi all, Is it possible to de-stain HE stained paraffin sections and re-use them for IHC staining??? Does anything special have to be done? Will it only work for some epitopes/stains? Does anyone have suggestions on how to do this? I am being asked to do this and don't want to waste my time if it won't work. I would appreciate any suggestions anyone might have Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_d...@compbio.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Competancies for handling hazardous material
Has anyone been inspected by the EPA regarding removal of hazardous chemicals? It came up in a safety meeting that I should have training and competencies (beyond general safety training and PPE) for pouring xylene from staining dishes and processors into accumulation containers. Rae Ann Staskiewicz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sirius red stain - picric acid substitute - water -really?
Hi Mia, In response to your question about the need for picric acid in the sirus red solution. Yes, you do need it. The article you listed was working with collagen gels and not actually tissue sections. If you omit the picric acid you not only loose the yellow counter stain as mentioned in the article, but the sirius red dye binds to other tissue components. It is also critical that the pH of the solution be between 2.0 and 2.3 (picric acid or not) in order for the staining to be specific to these collagen types. If you decide to try this please do so in parallel with the established picric acid technique and certainly make sure that the water is buffered to the necessary pH. I have a great article back in the lab about the stain and how it differentially stains the collagen types and bifringes to further differentiate them. It's a bit old, but definitely still relevant. Now I know there are some folks out there that will inevitably whine that picric acid is a hazardous chemical and shouldn't be used in the lab. Well guys it's a lab, that's just the way it goes. As long as you are conscious of the hazards involved, store, use and dispose of it correctly it can easily be used relatively safely. We all use gas in our car's gas tanks, and that is an extremely hazardous chemical. And some of us travel 75 MPH on the highway while texting and eating McDonalds with our kids fighting in the back seat. Being on the road with those folks is scarier than a bit of properly used picric acid. Just as you watch for that swerving minivan and use your car carefully, respect and use chemicals carefully and everything will be just fine. Amos Brooks On Wed, Feb 23, 2011 at 10:52 AM, histonet-requ...@lists.utsouthwestern.edu wrote: -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mia Woodruff Sent: Tuesday, February 22, 2011 10:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sirius red stain - picric acid substitute - water -really? Hello all, I have been undertaking sirius red staining using picric acid, I was under the impression (from papers) that picric acid is an important component of the procedure and read that it prevents non-specific binding of the dye to things other than collagen. However, I have recently found a paper which suggests I can simply use water instead of picric acid, seems quite a long shot but I wondered if anyone has experience with the water technique- given that picric acid is pretty dangerous I would be keen to move away from using it but only if scientifically sound to do so. I don't want to jeopardize my results but if this method works then it's a far safer and cheaper safer approach. Any advice? Paper: A specific quantitative assay for collagen synthesis by cells seeded in collagen-based biomaterials using sirius red F3B precipitation LEE D.A.; ASSOKU E.; DOYLE V. Journal of Materials Science: Materials in Medicinehttp://www.ingentaconnect.com/content/klu/jmsm, Volume 9, Number 1, 1998 , pp. 47-51(5) Many thanks Mia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sirius red stain - picric acid substitute
Hi Mia, I tried few different sources of Picric acid. Unfortunately, not all of them worked. The one that is working is from Rowley Biochemical Institute - www.rowleybio.com . I got a small bottle to try - 4 oz. Cat # SO-128. You can probably find it from one of the large vendors. Just a helpful thought Michelle USC Keck School of Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Competancies for handling hazardous material
Rae We have, you need standard training, but the individuals who inspected our lab did not mention compentances. But our inspection happened because we needed to switch from a conditionally exempt small quantity generator to a small quantitiy generator and we then needed to register with the state, etc. They primarily focused on our waste streams and what chemicals were in the lab. I'm not at work right now, but I'll check tomorrow to see if I have anything that I can share with you. Liz From: histonet-boun...@lists.utsouthwestern.edu on behalf of Rae Staskiewicz Sent: Wed 2/23/2011 6:19 PM To: Histonet Subject: [Histonet] Competancies for handling hazardous material Has anyone been inspected by the EPA regarding removal of hazardous chemicals? It came up in a safety meeting that I should have training and competencies (beyond general safety training and PPE) for pouring xylene from staining dishes and processors into accumulation containers. Rae Ann Staskiewicz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
