RE: [Histonet] Competancies for handling hazardous material

2011-02-23 Thread Liz Chlipala
Rae
 
We have, you need standard training, but the individuals who inspected our lab 
did not mention compentances.  But our inspection happened because we needed to 
switch from a conditionally exempt small quantity generator to a small 
quantitiy generator and we then needed to register with the state, etc.  They 
primarily focused on our waste streams and what chemicals were in the lab.  I'm 
not at work right now, but I'll check tomorrow to see if I have anything that I 
can share with you.
 
Liz



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Rae Staskiewicz
Sent: Wed 2/23/2011 6:19 PM
To: Histonet
Subject: [Histonet] Competancies for handling hazardous material





Has anyone been inspected by the EPA regarding removal of hazardous
chemicals?  It came up in a safety meeting that I should have training and
competencies (beyond general safety training and PPE) for pouring xylene
from staining dishes and processors into accumulation containers. 



Rae Ann Staskiewicz

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[Histonet] Sirius red stain - picric acid substitute

2011-02-23 Thread Michelle MacVeigh-Aloni
Hi Mia,

 

I tried few different sources of Picric acid. Unfortunately, not all of them
worked. The one that is working is from Rowley Biochemical Institute -
www.rowleybio.com . I got a small bottle to try - 4 oz. Cat # SO-128. You
can probably find it from one of the large vendors. 

 

Just a helpful thought

Michelle

USC Keck School of Medicine

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[Histonet] Sirius red stain - picric acid substitute - water -really?

2011-02-23 Thread Amos Brooks
Hi Mia,
 In response to your question about the need for picric acid in the
sirus red solution. Yes, you do need it. The article you listed was working
with collagen gels and not actually tissue sections. If you omit the picric
acid you not only loose the yellow counter stain as mentioned in the
article, but the sirius red dye binds to other tissue components. It is also
critical that the pH of the solution be between 2.0 and 2.3 (picric acid or
not) in order for the staining to be specific to these collagen types. If
you decide to try this please do so in parallel with the established picric
acid technique and certainly make sure that the water is buffered to the
necessary pH. I have a great article back in the lab about the stain and how
it differentially stains the collagen types and bifringes to further
differentiate them. It's a bit old, but definitely still relevant.
 Now I know there are some folks out there that will inevitably whine
that picric acid is a hazardous chemical and shouldn't be used in the lab.
Well guys it's a lab, that's just the way it goes. As long as you are
conscious of the hazards involved, store, use and dispose of it correctly it
can easily be used relatively safely. We all use gas in our car's gas tanks,
and that is an extremely hazardous chemical. And some of us travel 75 MPH on
the highway while texting and eating McDonalds with our kids fighting in the
back seat. Being on the road with those folks is scarier than a bit of
properly used picric acid. Just as you watch for that swerving minivan and
use your car carefully, respect and use chemicals carefully and everything
will be just fine.

Amos Brooks

On Wed, Feb 23, 2011 at 10:52 AM,  wrote:

> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mia
> Woodruff
> Sent: Tuesday, February 22, 2011 10:33 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Sirius red stain - picric acid substitute - water
> -really?
>
> Hello all,
>
> I have been undertaking sirius red staining using picric acid, I was under
> the
> impression (from papers) that picric acid is an important component of the
> procedure and read that it prevents non-specific binding of the dye to
> things
> other than collagen. However,  I have recently found a paper which suggests
> I
> can simply use water instead of picric acid, seems quite a long shot but I
> wondered if anyone has experience with the water technique- given that
> picric
> acid is pretty dangerous I would be keen to move away from using it but
> only if
> scientifically sound to do so. I don't want to jeopardize my results but if
> this
> method works then it's a far safer and cheaper safer approach. Any advice?
>
> Paper: A specific quantitative assay for collagen synthesis by cells seeded
> in
> collagen-based biomaterials using sirius red F3B precipitation
> LEE D.A.; ASSOKU E.; DOYLE V. Journal of Materials Science: Materials in
> Medicine, Volume 9, Number
> 1,
> 1998 , pp. 47-51(5)
>
>
> Many thanks
> Mia
>
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[Histonet] Competancies for handling hazardous material

2011-02-23 Thread Rae Staskiewicz
 

Has anyone been inspected by the EPA regarding removal of hazardous
chemicals?  It came up in a safety meeting that I should have training and
competencies (beyond general safety training and PPE) for pouring xylene
from staining dishes and processors into accumulation containers.  

 

Rae Ann Staskiewicz

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Re: [Histonet] use of destained sections in IHC?

2011-02-23 Thread Joe Nocito
I wouldn't decolorize the slides. Just remove the coverslip and hydrate to 
water. Any antigen retrieval should take out the Eosin. You're going to 
counterstain in Hematoxylin any way. Years ago (ok, many moons ago) I did an 
informal study. I took 10 antibodies, stained two sets of controls H & E. 
One set, I used 1% acid-alcohol to decolorize the slides, the other set I 
just rehydrated to water. The slides that were placed in acid-alcohol either 
had no staining or a marked decrease in intensity.


Joe
- Original Message - 
From: "Walter Benton" 
To: "Robin Dean" ; 


Sent: Wednesday, February 23, 2011 2:00 PM
Subject: RE: [Histonet] use of destained sections in IHC?


It will work and if you are performing antigen retrieval then you won't need 
to decolorize the slide, just rehydrate and perform per you usual protocol.


Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robin Dean 
[robin_d...@compbio.com]

Sent: Wednesday, February 23, 2011 2:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] use of destained sections in IHC?

Hi all,



Is it possible to de-stain H&E stained  paraffin sections and re-use them
for IHC staining??? Does anything special have to be done? Will it only work
for some epitopes/stains? Does anyone have suggestions on how to do this? I
am being asked to do this and don't want to waste my time if it won't work.
I would appreciate any suggestions anyone might have



Thank you,



Robin



Robin R. Dean, Ph.D.

Senior Scientist & Study Director

Comparative Biosciences, Inc.

786 Lucerne Dr.

Sunnyvale, CA

(408) 738-8060

robin_d...@compbio.com



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Re: [Histonet] RE: alcian blue-PAS control

2011-02-23 Thread Jennifer MacDonald
Cervix (with both endocervix and ectocervix) contains both glycogen (ecto) 
and mucin (endo).

Jennifer




Tony Henwood  
Sent by: histonet-boun...@lists.utsouthwestern.edu
02/23/2011 01:56 PM

To
"'Kuhnla, Melissa'" , 
"histonet@lists.utsouthwestern.edu" 
cc

Subject
[Histonet] RE: alcian blue-PAS control






Melissa,

We use a multi-tissue control.
Our control block contains appendix, umbilical cord and liver (for 
diastase).

The optimum control (if you can get it) would be an intestinal metaplasia 
of the stomach and combine this with a mucin-producing mesothelioma.

To you all, any further ideas?

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kuhnla, 
Melissa
Sent: Wednesday, 23 February 2011 11:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] alcian blue-PAS control

Hello,
Can anyone recommend a good control tissue to use for Alcian blue-PAS?

Melissa Kuhnla
Lead Medical Technologist for IHC and FISH Catholic Health Services of 
Long Island Regional Laboratory Services



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Re: [Histonet] use of destained sections in IHC?

2011-02-23 Thread Richard Cartun
Yes, you can use H&E-stained slides for IHC; however, if your lesion/tumor is 
negative and there is no internal positive control, then you may be dealing 
with a false negative result.  Having said that, we have had good luck with the 
majority of proteins we have tested.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> "Robin Dean"  2/23/2011 2:43 PM >>>
Hi all,

 

Is it possible to de-stain H&E stained  paraffin sections and re-use them
for IHC staining??? Does anything special have to be done? Will it only work
for some epitopes/stains? Does anyone have suggestions on how to do this? I
am being asked to do this and don't want to waste my time if it won't work.
I would appreciate any suggestions anyone might have 

 

Thank you,

 

Robin

 

Robin R. Dean, Ph.D.

Senior Scientist & Study Director

Comparative Biosciences, Inc.

786 Lucerne Dr.

Sunnyvale, CA

(408) 738-8060

robin_d...@compbio.com 

 

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[Histonet] RE: alcian blue-PAS control

2011-02-23 Thread Tony Henwood
Melissa,

We use a multi-tissue control.
Our control block contains appendix, umbilical cord and liver (for diastase).

The optimum control (if you can get it) would be an intestinal metaplasia of 
the stomach and combine this with a mucin-producing mesothelioma.

To you all, any further ideas?

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa
Sent: Wednesday, 23 February 2011 11:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] alcian blue-PAS control

Hello,
Can anyone recommend a good control tissue to use for Alcian blue-PAS?

Melissa Kuhnla
Lead Medical Technologist for IHC and FISH Catholic Health Services of Long 
Island Regional Laboratory Services



The information in this e-mail, and any attachments therein, is confidential 
and for use by the intended addressee only. If this message is received by you 
in error please do not disseminate or read further. Please reply to the sender 
that you have received the message in error, then delete the message. Although 
Catholic Health Services of Long Island attempts to sweep e-mail  and 
attachments for viruses, it does not guarantee that either are virus-free and 
accepts no liability for any damage sustained as a result of viruses. Thank you.

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Views expressed in this message and any attachments are those of the individual 
sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been virus scanned and 
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containing computer viruses.
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[Histonet] RE: Short term tissue storage PBS???

2011-02-23 Thread Anatoli Gleiberman
Jo-Ann,
Nothing is changed, but storage in PBS does not affect neither morphology nor 
antigens. I used to keep some neutral formalin- or formaldehyde-PBS fixed 
specimens  up to 1 year in sterile PBS at +4 C without any visible changes. So 
- don't worry. It will be fine.

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: agleiber...@cbiolabs.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jo-Ann Bader, 
Ms.
Sent: Wednesday, February 23, 2011 3:57 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Short term tissue storage PBS???

Hi All,

It has come to my attention recently that some of our histology clients are 
submitting their samples in PBS after fixation, instead of 70% alcohol.  When I 
ask them why they don't know and say they have been told to.  I have always  
followed the procedure that after fixation specimens are stored, short term in 
70% alcohol, until you are ready to process them. Has something changed lately 
that I am not aware of?


Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University 1160 Pine Ave. W - Rm 312 (3355)
Montreal, QC, Canada
H3G 1Y6
Tel: 514-398-8270
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Re: [Histonet] Short term tissue storage PBS???

2011-02-23 Thread Rene J Buesa
Nothing has changed, it is just "one of those things" people do because of 
fundamental ignorance.
PBS is not a preservative and could even favor bacterial growth (depending of 
the time elapsed).
After proper fixation, tissues should be placed in EthOL 70% as a general 
practice.René J.

 

--- On Wed, 2/23/11, Jo-Ann Bader, Ms.  wrote:


From: Jo-Ann Bader, Ms. 
Subject: [Histonet] Short term tissue storage PBS???
To: "Histonet@lists.utsouthwestern.edu" 
Date: Wednesday, February 23, 2011, 3:57 PM


Hi All,

It has come to my attention recently that some of our histology clients are 
submitting their samples in PBS after fixation, instead of 70% alcohol.  When I 
ask them why they don't know and say they have been told to.  I have always  
followed the procedure that after fixation specimens are stored, short term in 
70% alcohol, until you are ready to process them. Has something changed lately 
that I am not aware of?


Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University 1160 Pine Ave. W - Rm 312 (3355)
Montreal, QC, Canada
H3G 1Y6
Tel: 514-398-8270
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[Histonet] Short term tissue storage PBS???

2011-02-23 Thread Jo-Ann Bader, Ms.
Hi All,

It has come to my attention recently that some of our histology clients are 
submitting their samples in PBS after fixation, instead of 70% alcohol.  When I 
ask them why they don't know and say they have been told to.  I have always  
followed the procedure that after fixation specimens are stored, short term in 
70% alcohol, until you are ready to process them. Has something changed lately 
that I am not aware of?


Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University 1160 Pine Ave. W - Rm 312 (3355)
Montreal, QC, Canada
H3G 1Y6
Tel: 514-398-8270
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RE: [Histonet] IHC Equipment

2011-02-23 Thread Liz Chlipala
Cindy

We have three dako autostainers and we really like them a lot.  We are a
contract research lab so flexibility and system openness is key for us.
The only other instrument that I have demoed is the Leica Bond, but
unless I purchased the research software (which is a bit expensive) I
was tied into using at least one of their reagents and their reagents
are purchased in kits, so for my lab it was not a good fit financially
since I already had two dako stainers on reagent rental.

If you have the space I would demo the different instruments on the
market, see which one works best for you. 

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia
Pyse
Sent: Wednesday, February 23, 2011 12:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC Equipment

Hi Histonetters

I currently use a Dako stainer for my IHC staining. It is a work horse
with
very little problems. It is a older model that we may need to replace in
the
near future. What is everyone using out in histoland. I would be
perfectly
willing to purchase another Dako but I want to explore all avenues
before
making a decision. What are the pros and cons of the instruments any of
you
are using. How often is the machine down? What is the capacity? We run
the
Dako twice daily usually to the capacity of 48 slides. I would like to
hear
only from actual user of the instrumentation, no vendors please. This is
only a fact finding e-mail. Thanks in advance for all your input.

Cindy

 

Cindy Pyse, CLT, HT (ASCP)

Laboratory/Histology Supervisor

X-Cell Laboratories

716-250-9235

e-mail cp...@x-celllab.com

 

 

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[Histonet] RRAS

2011-02-23 Thread sgoebel
So...trying to find someone who sells RRAS antibody that isn't Abcam.  I
can't seem to make the Abcam antibody work and I have been asked to try
and get it from a different company.

 

2.  Does anyone have any experience with RRAS?  I have tried dilutions
from 1:500 all the way up to 1:2000.  I keep getting a blush endogenous
stain that I can't seem to get out even after blocking for everything
under the sun.  I am using a normal pH antigen retrieval (DAKO).  Should
I try high pH?  I don't know if that will make the positive staining
come out, I think it will make it worse?

 

Thanks histo-hotties!!

 

Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

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RE: [Histonet] use of destained sections in IHC?

2011-02-23 Thread Walter Benton
It will work and if you are performing antigen retrieval then you won't need to 
decolorize the slide, just rehydrate and perform per you usual protocol.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robin Dean 
[robin_d...@compbio.com]
Sent: Wednesday, February 23, 2011 2:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] use of destained sections in IHC?

Hi all,



Is it possible to de-stain H&E stained  paraffin sections and re-use them
for IHC staining??? Does anything special have to be done? Will it only work
for some epitopes/stains? Does anyone have suggestions on how to do this? I
am being asked to do this and don't want to waste my time if it won't work.
I would appreciate any suggestions anyone might have



Thank you,



Robin



Robin R. Dean, Ph.D.

Senior Scientist & Study Director

Comparative Biosciences, Inc.

786 Lucerne Dr.

Sunnyvale, CA

(408) 738-8060

robin_d...@compbio.com



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RE: [Histonet] use of destained sections in IHC?

2011-02-23 Thread Sebree Linda A
Robin,
We just take the coverslip/film off, hydrate to water and run for IHC
BUT many times the H&E slide is not a "+" slide so we end up losing
tissue.  It is not necessary to destain the H&E first.


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robin
Dean
Sent: Wednesday, February 23, 2011 1:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] use of destained sections in IHC?

Hi all,

 

Is it possible to de-stain H&E stained  paraffin sections and re-use
them
for IHC staining??? Does anything special have to be done? Will it only
work
for some epitopes/stains? Does anyone have suggestions on how to do
this? I
am being asked to do this and don't want to waste my time if it won't
work.
I would appreciate any suggestions anyone might have 

 

Thank you,

 

Robin

 

Robin R. Dean, Ph.D.

Senior Scientist & Study Director

Comparative Biosciences, Inc.

786 Lucerne Dr.

Sunnyvale, CA

(408) 738-8060

robin_d...@compbio.com

 

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Re: [Histonet] Joint Comm and patient identifiers

2011-02-23 Thread DKBoyd
The Joint Commission web site: 
http://www.jointcommission.org/accreditation/accreditation_main.aspx
click on Standards  and then click on National Patient Safety Goals.  It 
is the first standard in the chapter.
Standard number:  NPSG.01.01.01

Debbie M. Boyd, HT(ASCP) l Chief Hitologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkb...@chs.net







 
Sent by: histonet-boun...@lists.utsouthwestern.edu
02/23/2011 02:40 PM

To
"'histonet'" 
cc

Subject
[Histonet] Joint Comm and patient identifiers






Hi Histonetters!

 

I have a question related to the two patient identifiers that TJC 
requires:
can anyone point (online) me to the actual regulation?

 

It was my understanding that the 2 identifiers related to the *collection*
of the specimen, meaning that the container and associated requisition had
to have 2 positive patient identifiers.  The question is, do they
*specifically* state that the 2 identifiers must be carried through to the
final surgical slide?

 

The reason I ask is that I have a friend who got dinged for their slides 
not
having 2 patient identifiers on them.  They have the surgical number and
name of institution, but not the patient name or MRN.  My friend is just
looking for the actual statute so that he can read and follow exactly as
expected.

 

Also, can anyone confirm that the surgical number and a bar code would
suffice as 2 identifiers?

 

Thanks! 

 

Michelle

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Re: [Histonet] CAP

2011-02-23 Thread Angela Bitting
I did notice CAP inspectors concentrated more on safety this time around.

>>> Akemi Allison  2/23/2011 11:50 AM >>>
We were given the new CAP checklist.  I totally revamped our SOP Manuals, ALL 
QC 
forms, etc. to comply.  


This is the 1st Childrens Hospital CAP inspection I have undergone.  We are 
inspected by other Children Hospital inspecting teams.  


After all the hard work, they did not go through any of our SOP's, QC manuals, 
Special Stains, validation protocols manuals for either histology or the IHC 
lab.  Very odd indeed.  


They spoke with the AP manager for our department only, except to ask one of 
the 
histology techs how we disposed of our hazardous waste. Stay tuned till after 
the summation.
Akemi Allison BS, HT(ASCP)HTL








From: Jesus Ellin 
To: Akemi Allison ; histonet 

Sent: Wed, February 23, 2011 7:23:43 AM
Subject: RE: [Histonet] CAP

Akemi, were you inspected with the new CAP checklist or the old one?
The situation in our lab is that we were given the checklist and then
they changed us to the new format.  Your thoughts

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi
Allison
Sent: Wednesday, February 23, 2011 7:03 AM
To: histonet
Subject: [Histonet] CAP

Lot's of Labs in LA are in their CAP window!  We had our CAP inspection 
yesterday and having our summation this morning at 9:00.  I think our
department 
did pretty good.  Keeping my fingers crossed.  
Akemi Allison BS, HT(ASCP)HTL


  
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[Histonet] use of destained sections in IHC?

2011-02-23 Thread Robin Dean
Hi all,

 

Is it possible to de-stain H&E stained  paraffin sections and re-use them
for IHC staining??? Does anything special have to be done? Will it only work
for some epitopes/stains? Does anyone have suggestions on how to do this? I
am being asked to do this and don't want to waste my time if it won't work.
I would appreciate any suggestions anyone might have 

 

Thank you,

 

Robin

 

Robin R. Dean, Ph.D.

Senior Scientist & Study Director

Comparative Biosciences, Inc.

786 Lucerne Dr.

Sunnyvale, CA

(408) 738-8060

robin_d...@compbio.com

 

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[Histonet] IHC Equipment

2011-02-23 Thread Cynthia Pyse
Hi Histonetters

I currently use a Dako stainer for my IHC staining. It is a work horse with
very little problems. It is a older model that we may need to replace in the
near future. What is everyone using out in histoland. I would be perfectly
willing to purchase another Dako but I want to explore all avenues before
making a decision. What are the pros and cons of the instruments any of you
are using. How often is the machine down? What is the capacity? We run the
Dako twice daily usually to the capacity of 48 slides. I would like to hear
only from actual user of the instrumentation, no vendors please. This is
only a fact finding e-mail. Thanks in advance for all your input.

Cindy

 

Cindy Pyse, CLT, HT (ASCP)

Laboratory/Histology Supervisor

X-Cell Laboratories

716-250-9235

e-mail cp...@x-celllab.com

 

 

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[Histonet] Joint Comm and patient identifiers

2011-02-23 Thread histotech
Hi Histonetters!

 

I have a question related to the two patient identifiers that TJC requires:
can anyone point (online) me to the actual regulation?

 

It was my understanding that the 2 identifiers related to the *collection*
of the specimen, meaning that the container and associated requisition had
to have 2 positive patient identifiers.  The question is, do they
*specifically* state that the 2 identifiers must be carried through to the
final surgical slide?

 

The reason I ask is that I have a friend who got dinged for their slides not
having 2 patient identifiers on them.  They have the surgical number and
name of institution, but not the patient name or MRN.  My friend is just
looking for the actual statute so that he can read and follow exactly as
expected.

 

Also, can anyone confirm that the surgical number and a bar code would
suffice as 2 identifiers?

 

Thanks!  

 

Michelle

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Re: [Histonet] CAP

2011-02-23 Thread Jay Lundgren
 If your lab smells like oranges, just open your CAP window and air it
out.


  Sincerely,

  Jay A.
Lundgren M.S., HTL (ASCP)
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[Histonet] Diff Quik/Wright stain for stool samples

2011-02-23 Thread Debra Siena
Hello All,

Does anyone use a Diff Quik protocol for parasites in stool samples and if so 
could you send me a copy of your procedure?  I would greatly appreciate it.  
Thank you all.

Debbie Siena HT(ASCP)QIHC
Technical Manager | StatLab Medical Products
407 Interchange St. | McKinney, TX 75071
Direct: 972-436-1010  x229 | Fax: 972-436-1369
dsi...@statlab.com | 
www.statlab.com


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RE: [Histonet] Bone marrow charges

2011-02-23 Thread Rathborne, Toni
Are your blocks (1 bx and 1 clot) separate specimens, or just one specimen with 
2 blocks?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Goodwin,
Diana
Sent: Monday, February 21, 2011 11:40 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Bone marrow charges


Greetings, Histonetters.

How many times can I charge 88313 for a bone marrow case that has an Iron stain 
on 2 separate blocks (one for the bx and one for the aspirate clot) and also on 
1 smear made from the aspirate clot, a PAS on the 2 blocks, a Trichrome and a 
Retic on the bx. block, and a Wright/ Giemsa on 4 smears?

Thank you!!!
Diana Goodwin
Supervisor, Histology Laboratory
RWJUHHNJ


Diana Goodwin
Supervisor, Histology Laboratory
xt. 6996

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[Histonet] Re: LCM & archival FFPE tissue (Margaryan, Naira)

2011-02-23 Thread Suresch, Donna L.
I have used archival FFPE slides that had used antigen retrieval methods for 
LCM and the yields seemed to be good.  The RNA/DNA yields obviously depend on 
how many cells you are collecting.  We routinely used 8um sections for the LCM 
to increase yields.  If you are interested in collecting a population that is 
very limited in your samples (i.e., ER+ or PR+ in small tumors), you will need 
to have maybe 20 or more slides (estimate).  I would collect those slides into 
a single microcentrifuge tube (insert LCM cap and invert it to get cells into 
buffer) using lysing buffer, heat at 42C for 30 min, spin at 2000x g for 1 min, 
decant buffer, resuspend pellet into RLT/70% ethanol, and freeze the samples at 
-80 degrees C until ready for PCR.
There are some excellent protocols from Quiagen online that will be helpful.  
Hope this helps.


Donna L. Suresch
Merck Research Laboratories
Research Biologist
Imaging Research - West Point
Mail Stop:  WP44KOffice: WP44-H129
770 Sumneytown Pike
PO Box 4
West Point, PA  19486-0004
Phone:  215-652-7349
Fax:  215-993-6803
 <> 
Message: 4
Date: Tue, 22 Feb 2011 13:26:47 -0600
From: "Margaryan, Naira" 
Subject: [Histonet] LCM & archival FFPE tissue
To: "histonet@lists.utsouthwestern.edu"




Message-ID:







Content-Type: text/plain; charset="utf-8"

Hi all,

I have a couple questions I would like to throw out to the experts:

1)  1) Is it possible to extract RNA and DNA for gene expression purpose 
after usual IHC procedure on 4um sections of archival FFPE breast tumor tissue 
(If tissue was in citrate buffer, pH 6.0; several blocking steps; one hour in 
primary Ab (less then one hour will not work); with immunoperoxidase-DAB 
detection follow the Hematoxylin?
2) Or I need only H&E?
2)  3) Is 4-5 um section good enough?
3)  4) How many sections need to have really good and highest yields?
4)  5) What temperature of Proteinase K is need 42º or 60ºC?
5)
I've the LCM system from Arcturus and the Pico Pure Kit.

Any suggestions?

Thanks in advance,
Naira


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Re: [Histonet] CAP

2011-02-23 Thread Akemi Allison
We were given the new CAP checklist.  I totally revamped our SOP Manuals, ALL 
QC 
forms, etc. to comply.  


This is the 1st Childrens Hospital CAP inspection I have undergone.  We are 
inspected by other Children Hospital inspecting teams.  


After all the hard work, they did not go through any of our SOP's, QC manuals, 
Special Stains, validation protocols manuals for either histology or the IHC 
lab.  Very odd indeed.  


They spoke with the AP manager for our department only, except to ask one of 
the 
histology techs how we disposed of our hazardous waste. Stay tuned till after 
the summation.
 Akemi Allison BS, HT(ASCP)HTL








From: Jesus Ellin 
To: Akemi Allison ; histonet 

Sent: Wed, February 23, 2011 7:23:43 AM
Subject: RE: [Histonet] CAP

Akemi, were you inspected with the new CAP checklist or the old one?
The situation in our lab is that we were given the checklist and then
they changed us to the new format.  Your thoughts

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi
Allison
Sent: Wednesday, February 23, 2011 7:03 AM
To: histonet
Subject: [Histonet] CAP

Lot's of Labs in LA are in their CAP window!  We had our CAP inspection 
yesterday and having our summation this morning at 9:00.  I think our
department 
did pretty good.  Keeping my fingers crossed.  
Akemi Allison BS, HT(ASCP)HTL


      
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RE: [Histonet] Digital Pathology/Telepathology

2011-02-23 Thread Liz Chlipala
Andrew

We are a research contract lab and have been scanning slides (WSI) since
2007.  We have an Aperio Scanscope XT.  Our clients have secure remote
access to their images.  We manage our own server, storage (20TB) and
backup locally.  

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrew
Byrnes
Sent: Wednesday, February 23, 2011 6:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Digital Pathology/Telepathology

Dear Histonet,

Please don't take this as spam.  I am just gathering some information...

I am curious to know if any of you are currently working with digital  
whole slide scanners and what your experience has been to date.

Additionally, my company provides telepathology services by putting a  
digital scanner into your lab allowing our pathology group to access  
electronic requisitions and digital slides remotely.  We then return a  
report electronically within 24 hours.  All is stored on our servers  
for secure access from anywhere at anytime.  The idea is to allow easy  
access to our fully sub-specialized pathology group by any  
laboratories that may have a need for more consistent pathology  
coverage or if there are quality issues.  Same concept as  
teleradiology (Virtual Radiologic, Nighthawk Radiology) which is  
implemented in many hospitals throughout the US.  If interested, our  
website is www.accelpath.com

Would love to hear your thoughts on these subjects... digital path and  
telepath.

Have a great day!
Andrew Byrnes
AccelPath





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Re: [Histonet] CAP

2011-02-23 Thread DKBoyd
That's a good thing!  That means you need more ventilation and admin 
listens when inspectors speak!


Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkb...@chs.net







"Angela Bitting"  
Sent by: histonet-boun...@lists.utsouthwestern.edu
02/23/2011 10:30 AM

To
"histonet" , "Akemi Allison" 

cc

Subject
Re: [Histonet] CAP







We just had ours last week.
We did well, but the inspecting Pathologist said the lab smelled like 
oranges we were in a way too cramped space. Go figure..
 

>>> Akemi Allison  2/23/2011 9:02 AM >>>
Lot's of Labs in LA are in their CAP window!  We had our CAP inspection 
yesterday and having our summation this morning at 9:00.  I think our 
department 
did pretty good.  Keeping my fingers crossed. 
Akemi Allison BS, HT(ASCP)HTL


 
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[Histonet] RE: [IHCRG] AMACR (P504S)

2011-02-23 Thread Drew Sally A
We currently use Cat#Z2001, clone 13H4 from Zeta Corp with great
results, although we only use it for HMWCK/p63/P504s stain.  We have
also used a predilute (PP200) from BioCare (that worked quite well).  
 

Sally 

 




From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On
Behalf Of Taylor, Jean
Sent: Wednesday, February 23, 2011 9:41 AM
To: histonet@lists.utsouthwestern.edu; ih...@googlegroups.com
Subject: [IHCRG] AMACR (P504S)



Hi Everyone,

 

Our pathologists are interested in using this antibody to detect
metastatic renal cell carcinoma in paraffin sections. They are not
interested in an antibody cocktail, but also want to use it to detect
prostate cancer. I'd like to know what company and clone most labs are
using. 

 

Thanks!

 

Jean Taylor, HT(ASCP)QIHC

IHC Tech

Meriter Health Services

Madison, WI



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[Histonet] AMACR (P504S)

2011-02-23 Thread Taylor, Jean
Hi Everyone,

Our pathologists are interested in using this antibody to detect metastatic 
renal cell carcinoma in paraffin sections. They are not interested in an 
antibody cocktail, but also want to use it to detect prostate cancer. I'd like 
to know what company and clone most labs are using.

Thanks!

Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI
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RE: [Histonet] CAP

2011-02-23 Thread sgoebel
What?!?  A histology lab with no enough space...who ever heard of such a
thing 

Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela
Bitting
Sent: Wednesday, February 23, 2011 9:29 AM
To: histonet; Akemi Allison
Subject: Re: [Histonet] CAP


We just had ours last week.
We did well, but the inspecting Pathologist said the lab smelled like
oranges we were in a way too cramped space. Go figure..
 

>>> Akemi Allison  2/23/2011 9:02 AM >>>
Lot's of Labs in LA are in their CAP window!  We had our CAP inspection 
yesterday and having our summation this morning at 9:00.  I think our
department 
did pretty good.  Keeping my fingers crossed.  
Akemi Allison BS, HT(ASCP)HTL


  
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RE: [Histonet] CAP

2011-02-23 Thread Wanda.Smith
Sometimes it is a good thing when CAP says you are cramped for space.  It 
"sometimes" makes the hospital Administration wake up and take notice!!!
W

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting
Sent: Wednesday, February 23, 2011 10:29 AM
To: histonet; Akemi Allison
Subject: Re: [Histonet] CAP


We just had ours last week.
We did well, but the inspecting Pathologist said the lab smelled like oranges 
we were in a way too cramped space. Go figure..
 

>>> Akemi Allison  2/23/2011 9:02 AM >>>
Lot's of Labs in LA are in their CAP window!  We had our CAP inspection 
yesterday and having our summation this morning at 9:00.  I think our 
department 
did pretty good.  Keeping my fingers crossed.  
Akemi Allison BS, HT(ASCP)HTL


  
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Re: [Histonet] CAP

2011-02-23 Thread Angela Bitting

We just had ours last week.
We did well, but the inspecting Pathologist said the lab smelled like oranges 
we were in a way too cramped space. Go figure..
 

>>> Akemi Allison  2/23/2011 9:02 AM >>>
Lot's of Labs in LA are in their CAP window!  We had our CAP inspection 
yesterday and having our summation this morning at 9:00.  I think our 
department 
did pretty good.  Keeping my fingers crossed.  
Akemi Allison BS, HT(ASCP)HTL


  
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RE: [Histonet] CAP

2011-02-23 Thread Jesus Ellin
Akemi, were you inspected with the new CAP checklist or the old one?
The situation in our lab is that we were given the checklist and then
they changed us to the new format.  Your thoughts

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi
Allison
Sent: Wednesday, February 23, 2011 7:03 AM
To: histonet
Subject: [Histonet] CAP

Lot's of Labs in LA are in their CAP window!  We had our CAP inspection 
yesterday and having our summation this morning at 9:00.  I think our
department 
did pretty good.  Keeping my fingers crossed.  
 Akemi Allison BS, HT(ASCP)HTL


  
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RE: [Histonet] Sirius red stain - picric acid substitute - water -really?

2011-02-23 Thread Marcum, Pamela A
Since you can buy a saturated Picric acid solution and I have for years why are 
you worried?  It is stable in a saturated liquid and you can purchase fairly 
small amounts at a time.  I only worry if find some in a powder form turning a 
nasty colour.  It has been about twenty five years since I had that happen and 
immediately went to pre-mixed solutions.

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood, 
Margaret 
Sent: Wednesday, February 23, 2011 8:04 AM
To: Mia Woodruff; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Sirius red stain - picric acid substitute - water 
-really?

I would do a trial run on "extra" slides or non-valuable tissue? 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mia Woodruff
Sent: Tuesday, February 22, 2011 10:33 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Sirius red stain - picric acid substitute - water -really?

Hello all,

I have been undertaking sirius red staining using picric acid, I was under the
impression (from papers) that picric acid is an important component of the
procedure and read that it prevents non-specific binding of the dye to things
other than collagen. However,  I have recently found a paper which suggests I
can simply use water instead of picric acid, seems quite a long shot but I
wondered if anyone has experience with the water technique- given that picric
acid is pretty dangerous I would be keen to move away from using it but only if
scientifically sound to do so. I don't want to jeopardize my results but if this
method works then it's a far safer and cheaper safer approach. Any advice?

Paper: A specific quantitative assay for collagen synthesis by cells seeded in
collagen-based biomaterials using sirius red F3B precipitation
LEE D.A.; ASSOKU E.; DOYLE V. Journal of Materials Science: Materials in
Medicine, Volume 9, Number 1,
1998 , pp. 47-51(5)


Many thanks
Mia





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RE: [Histonet] CAP

2011-02-23 Thread Wanda.Smith
Akemi,
Keep us informed!!!  I too, am in my CAP window!!!
Thanks,
Wanda

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison
Sent: Wednesday, February 23, 2011 9:03 AM
To: histonet
Subject: [Histonet] CAP

Lot's of Labs in LA are in their CAP window!  We had our CAP inspection 
yesterday and having our summation this morning at 9:00.  I think our 
department 
did pretty good.  Keeping my fingers crossed.  
 Akemi Allison BS, HT(ASCP)HTL


  
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RE: [Histonet] Sirius red stain - picric acid substitute - water -really?

2011-02-23 Thread Sherwood, Margaret
I would do a trial run on "extra" slides or non-valuable tissue? 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mia Woodruff
Sent: Tuesday, February 22, 2011 10:33 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Sirius red stain - picric acid substitute - water -really?

Hello all,

I have been undertaking sirius red staining using picric acid, I was under the
impression (from papers) that picric acid is an important component of the
procedure and read that it prevents non-specific binding of the dye to things
other than collagen. However,  I have recently found a paper which suggests I
can simply use water instead of picric acid, seems quite a long shot but I
wondered if anyone has experience with the water technique- given that picric
acid is pretty dangerous I would be keen to move away from using it but only if
scientifically sound to do so. I don't want to jeopardize my results but if this
method works then it's a far safer and cheaper safer approach. Any advice?

Paper: A specific quantitative assay for collagen synthesis by cells seeded in
collagen-based biomaterials using sirius red F3B precipitation
LEE D.A.; ASSOKU E.; DOYLE V. Journal of Materials Science: Materials in
Medicine, Volume 9, Number 1,
1998 , pp. 47-51(5)


Many thanks
Mia





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[Histonet] CAP

2011-02-23 Thread Akemi Allison
Lot's of Labs in LA are in their CAP window!  We had our CAP inspection 
yesterday and having our summation this morning at 9:00.  I think our 
department 
did pretty good.  Keeping my fingers crossed.  
 Akemi Allison BS, HT(ASCP)HTL


  
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[Histonet] Digital Pathology/Telepathology

2011-02-23 Thread Andrew Byrnes

Dear Histonet,

Please don't take this as spam.  I am just gathering some information...

I am curious to know if any of you are currently working with digital  
whole slide scanners and what your experience has been to date.


Additionally, my company provides telepathology services by putting a  
digital scanner into your lab allowing our pathology group to access  
electronic requisitions and digital slides remotely.  We then return a  
report electronically within 24 hours.  All is stored on our servers  
for secure access from anywhere at anytime.  The idea is to allow easy  
access to our fully sub-specialized pathology group by any  
laboratories that may have a need for more consistent pathology  
coverage or if there are quality issues.  Same concept as  
teleradiology (Virtual Radiologic, Nighthawk Radiology) which is  
implemented in many hospitals throughout the US.  If interested, our  
website is www.accelpath.com


Would love to hear your thoughts on these subjects... digital path and  
telepath.


Have a great day!
Andrew Byrnes
AccelPath





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RE: [Histonet] IgG and IgM on mouse kidneys

2011-02-23 Thread Sheila Fonner
Liz,

You can perform IgG and IgM on FFPE tissue samples.  We do them through IHC
on our Ventana Ultra and get the antibodies from Cell Marque.  Our
pathologists are happy with them.

Sheila


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala
Sent: Saturday, February 19, 2011 5:59 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IgG and IgM on mouse kidneys

Hello all

 

I have a quick question.  Can I perform IgG and IgM staining on mouse
kidneys on FFPE tissue samples?  I thought that this type of staining is
normally completed on frozen sections via Immunofluorescence.  Any
advice would be helpful

 

Thanks

 

Liz

 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC

Manager

Premier Laboratory, LLC

PO Box 18592

Boulder, Colorado 80308

office (303) 682-3949 

fax (303) 682-9060

www.premierlab.com

 

 

Ship to Address:

1567 Skyway Drive, Unit E

Longmont, Colorado 80504

 

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[Histonet] FW: alcian blue-PAS control

2011-02-23 Thread Kuhnla, Melissa
Can anyone recommend tissue besides duodenum??

_
From: Kuhnla, Melissa 
Sent: Wednesday, February 23, 2011 7:37 AM
To: ' (histonet@lists.utsouthwestern.edu)'
Subject: alcian blue-PAS control

Hello,
Can anyone recommend a good control tissue to use for Alcian blue-PAS?

Melissa Kuhnla 
Lead Medical Technologist for IHC and FISH
Catholic Health Services of Long Island
Regional Laboratory Services



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[Histonet] alcian blue-PAS control

2011-02-23 Thread Kuhnla, Melissa
Hello,
Can anyone recommend a good control tissue to use for Alcian blue-PAS?

Melissa Kuhnla 
Lead Medical Technologist for IHC and FISH
Catholic Health Services of Long Island
Regional Laboratory Services



The information in this e-mail, and any attachments therein, is confidential 
and for use by the intended addressee only. If this message is received by you 
in error please do not disseminate or read further. Please reply to the sender 
that you have received the message in error, then delete the message. Although 
Catholic Health Services of Long Island attempts to sweep e-mail  and 
attachments for viruses, it does not guarantee that either are virus-free and 
accepts no liability for any damage sustained as a result of viruses. Thank you.

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