RE: [Histonet] Stupid Rabbit primaries!
Having read all the other comments, here's my 2.5 pence worth! Have you tried a background reducing antibody diluent? You can get these from DAKO or Menarini Diagnostics: http://www.dako.com/uk/ar38/p107410/prod_products.htm. Or MP-905-25 / MP-905-100, depending on the volume you require, from Menarini Diagnostics, website http://www.dako.com/uk/ar38/p107410/prod_products.htm. I found these useful for rabbit polyclonals. Good luck Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com Sent: 22 March 2011 21:04 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Stupid Rabbit primaries! So I haven't had to deal with rabbit polyclonal primaries in a long time because I remember how much the background sucks with them. Unfortunately the only available antibody is a rabbit polyclonal. Does anyone have any suggestions for how to eliminate the background? I have diluted almost to the point of the antigens not showing! Thanks guys and gals!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Rabbit Anti-NeuN
Dear All I am looking for Rabbit Anti- NeuN antiboy to use it with anti-mouse OX42 for double immuno staining. Is there any one who have used any Rabbit Anti- NeuN? Please let me know. Thanks Sohail ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: B5
We did until about 7 yrs ago when the hospital decided that all mercury (thermometers and chemicals) would no longer be allowed which was fine with us. We just went to 10% NBF since then. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pitts, Jaclyn S. (Jackie), HT(ASCP) Sent: Tuesday, March 22, 2011 9:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] B5 Hey all, I was just curious how many of you out there still use B5 as a fixative for bone marrows. Thank Jaclyn Pitts, HT(ASCP) Histotechnician Department of Laboratory Medicine and Pathology Mayo Clinic Rochester, MN E-mail: pitts.jac...@mayo.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pax 2
Is anyone using the Pax 2 antibody? Who is your vendor and could you share some information about your procedure? Thank you, Andrea O'Brien UroPartners ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventilated Specimen Storage
We have one in our gross room. Caula Gill(HT)ASCP -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, March 22, 2011 11:35 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Ventilated Specimen Storage Hello Histonetters, How many of you have ventilated storage cabinets for storage of specimens? Thanks in advance for all your help, Amy GHS NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] please remove me from list. Thank you.
No. And you're not welcome. It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins On Tue, Mar 22, 2011 at 9:34 PM, dmb...@aol.com wrote: Please remove me from list. Thank you. Dannie Blake ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: vytec formalin neutralizer
Nirmala We Use Vytec and after consulting with the city's public utility it is ok for us to dispose of the waste with copious amounts of water down the drain. So, I would contact you local water authority and see if you can do the same. Regards, Matt Lunetta BS HT(ASCP)cm Longmont United Hospital Longmont, Colorado Message: 4 Date: Tue, 22 Mar 2011 10:33:12 -0700 From: sris...@mail.holyname.org Subject: [Histonet] vytec formalin neutralizer To: histonet-boun...@lists.utsouthwestern.edu, Message-ID: Content-Type: text/plain; charset=US-ASCII Hi Everyone, Is there any one out there who is neutralizing the 10% formalin with Vytec neutralizer? How are you disposing the formalin after neutralization? Need some information regarding this. Thanks in advance Nirmala ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Underfixation of breast tissue may lead to false results?
Dear Histonetters: I recently read the following in an article on Medscape: Underfixation of breast tissue may lead to false negative ER results and false-positive HER2 results. In these situations, the tissue is actually fixed in 100% ethanol, which is used to dehydrate the specimens after fixation. Can anyone explain? Diana Goodwin Supervisor, Histology Laboratory xt. 6996 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] B5
We do! Our pathologists aren't interested in using any other fixative. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pitts, Jaclyn S. (Jackie), HT(ASCP) Sent: Tuesday, March 22, 2011 6:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] B5 Hey all, I was just curious how many of you out there still use B5 as a fixative for bone marrows. Thank Jaclyn Pitts, HT(ASCP) Histotechnician Department of Laboratory Medicine and Pathology Mayo Clinic Rochester, MN E-mail: pitts.jac...@mayo.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] B5
Who do you get to discard it? Do you pay a fortune? j -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, March 23, 2011 10:23 To: Pitts, Jaclyn S. (Jackie), HT(ASCP); histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] B5 We do! Our pathologists aren't interested in using any other fixative. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pitts, Jaclyn S. (Jackie), HT(ASCP) Sent: Tuesday, March 22, 2011 6:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] B5 Hey all, I was just curious how many of you out there still use B5 as a fixative for bone marrows. Thank Jaclyn Pitts, HT(ASCP) Histotechnician Department of Laboratory Medicine and Pathology Mayo Clinic Rochester, MN E-mail: pitts.jac...@mayo.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RELIA Histology Careers Bulletin 3/23/2011 March Madness is Here!!
Hi Histonetters! I hope you had a fun and lucky St. Patricks Day. March Madness has started and in addition to some great College Basketball. I have been experiencing the madness as well. My phone has literally been ringing off the hook with some great new opportunities. All of these positions are permanent full time positions and my clients offer excellent compensation, benefits and relocation assistance. Best of all these clients are motivated to hire and eager to meet you! Here is a list of my current openings: HISTOLOGY/PATHOLOGY MANAGEMENT ME Portland Histology Lab Supervisor (Night shift) NC Asheville Histology Lab Manager FL Sarasota Histology Supervisor/Lead Tech CA Modesto Histology Lab Manager NH Manchester Cytology/Histology Supervisor LA Baton Rouge Early Morning Histology Supervisor HISTOTECHS NC Charlotte Mohs Histotech TX Dallas Night Shift Histotech TX Tyler Dermpath Histotech New grads welcome! LA Lafayette Night Shift Lead Tech OR Portland Night Shift Histotech MA Cape Cod Histotech MA Peabody Night Shift Histotech FL Fort Myers Mohs Histotech FL Sarasota Dermpath histotech If you or anyone you know might be interested in any of these positions or want help with a job search in another area please contact me. I can be reached at 866-607-3542 or rel...@earthlink.net. Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Underfixation of breast tissue may lead to false results?
This has been commented on several times by old fogey histotechs in the past. Any formalin variant takes some time to actually chemically alter (fix) the tissue. Usually this is a minimum of 24 hours for a 3-4 mm thick piece. In modern labs there is a focus on 24 hour or less turnaround time, so formalin fixation is usually minimalist and may be inadequate, as in this case. After all, the universe will not change itself for our benefit just because we want fast results. This means that underfixed tissues with just a few hours in formalin are processed. The unfixed components are exposed to ethanol during this process. Remember that ethanol is also a (very poor) fixing agent, so the inadequatly fixed tissue is fixed by it, with all the characteristics ethanol fixation gives, shattering, hardening, distortion, etc. This is frequently referred to as overprocessing, but it is just poor fixation from ethanol. For most tissues, wetting the block surface allows reasonable sections to be cut, but that is only a quick fix. Procedures which require tissues to be properly fixed may not be successful as a consequence. Bryan Llewellyn Goodwin, Diana wrote: Dear Histonetters: I recently read the following in an article on Medscape: Underfixation of breast tissue may lead to false negative ER results and false-positive HER2 results. In these situations, the tissue is actually fixed in 100% ethanol, which is used to dehydrate the specimens after fixation. Can anyone explain? Diana Goodwin Supervisor, Histology Laboratory xt. 6996 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Underfixation of breast tissue may lead to falseresults?
Just wanted to qualify somewhat the observation that alcohol is a poor fixative. Alcohol can be very effective as a primary fixative. I agree formalin isn't likely to be displaced in this country soon, but aqueous ethanol or methanol is a viable alternative that has merits worth considering. Dean Troyer Eastern Virginia Medical School From: histonet-boun...@lists.utsouthwestern.edu on behalf of Bryan Llewellyn Sent: Wed 3/23/2011 1:28 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Underfixation of breast tissue may lead to falseresults? This has been commented on several times by old fogey histotechs in the past. Any formalin variant takes some time to actually chemically alter (fix) the tissue. Usually this is a minimum of 24 hours for a 3-4 mm thick piece. In modern labs there is a focus on 24 hour or less turnaround time, so formalin fixation is usually minimalist and may be inadequate, as in this case. After all, the universe will not change itself for our benefit just because we want fast results. This means that underfixed tissues with just a few hours in formalin are processed. The unfixed components are exposed to ethanol during this process. Remember that ethanol is also a (very poor) fixing agent, so the inadequatly fixed tissue is fixed by it, with all the characteristics ethanol fixation gives, shattering, hardening, distortion, etc. This is frequently referred to as overprocessing, but it is just poor fixation from ethanol. For most tissues, wetting the block surface allows reasonable sections to be cut, but that is only a quick fix. Procedures which require tissues to be properly fixed may not be successful as a consequence. Bryan Llewellyn Goodwin, Diana wrote: Dear Histonetters: I recently read the following in an article on Medscape: Underfixation of breast tissue may lead to false negative ER results and false-positive HER2 results. In these situations, the tissue is actually fixed in 100% ethanol, which is used to dehydrate the specimens after fixation. Can anyone explain? Diana Goodwin Supervisor, Histology Laboratory xt. 6996 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Stupid Rabbit Primary Antibodies
You might try Background Sniper from Biocare or Background Buster from Innovex - we process animal tissues exclusively and we use several rabbit primary antibodies. Tresa Goins, Ph.D. Histopathology Supervisor Department of Livestock Bozeman, Montana -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com Sent: Tuesday, March 22, 2011 3:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Stupid Rabbit primaries! So I haven't had to deal with rabbit polyclonal primaries in a long time because I remember how much the background sucks with them. Unfortunately the only available antibody is a rabbit polyclonal. Does anyone have any suggestions for how to eliminate the background? I have diluted almost to the point of the antigens not showing! Thanks guys and gals!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: B5
Thanks. I am not sure who disposes of the B5 for us but I do know that it would be much easier if we changed to something else. Jaclyn Pitts, HT(ASCP) Histotechnician Department of Laboratory Medicine and Pathology Mayo Clinic E-mail: pitts.jac...@mayo.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jeffery.mil...@spectrum-health.org Sent: Wednesday, March 23, 2011 7:56 AM To: Pitts, Jaclyn S. (Jackie), HT(ASCP); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: B5 We did until about 7 yrs ago when the hospital decided that all mercury (thermometers and chemicals) would no longer be allowed which was fine with us. We just went to 10% NBF since then. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pitts, Jaclyn S. (Jackie), HT(ASCP) Sent: Tuesday, March 22, 2011 9:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] B5 Hey all, I was just curious how many of you out there still use B5 as a fixative for bone marrows. Thank Jaclyn Pitts, HT(ASCP) Histotechnician Department of Laboratory Medicine and Pathology Mayo Clinic Rochester, MN E-mail: pitts.jac...@mayo.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Verhoeff/Masson's Stain
The comments on Verhoeff's hematoxylin should be sufficient to trouble-shoot this cumbersome but beautiful old stain - I don't know a stain I'd rather photograph, except maybe Ramón y Cajal's gold sublimate. Verhoeff's hematoxylin is a century old this year. The original paper is in the JAMA, of all places - I think I had a photocopy once. (We need a commemorative postage stamp!) Frederick Herman Verhoeff (a.k.a. Freddy, 1874-1968 - I've always heard it pronounced veer-hoff) was the founder of American ophthalmologic pathology. Working solo in a little lab at the Massachusetts Eye and Ear Infirmary, he published a great number of papers in ophthalmology. He intended his stain to demonstrate myelin sheaths in human autopsy optic nerve. It was in routine use for this purpose at the Wilmer Eye Institute at Johns Hopkins when I was a pathology resident there in the later 1960's, when Dick Green (died last year at 76) was the chief of eye pathology. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] B5
We switched a few years ago. Hospital went mercury free, and we couldn't find any company in 6 states that would take waste mercury. One common mistake when converting to a zinc formalin is to try to fix it the same time as the B5. Mercury binds/fixes very quickly, zinc is slower. It's chemistry, and there's not much you can do to make it go a whole lot faster. So, whatever time you fixed normally in B5, multiply it by 1.5 to 2.0 (2 hour B5 fix will now require 3-4 hours fixation in a Zinc formalin). If you don't fix long enough in the zinc formalin, the tissue is going to have smudgy pale blue nuclei on the bone marrow. Been there, done that when one of the med techs insisted on fixing in the zinc formalin for the same 2 hours as B5 because she didn't like her routine disrupted (B5 was 2 hours, so she insisted the zinc formalin should also be 2 hours). Once we showed her the difference in quality between 2 hours and 3 hours, and asked her which one she would like her daughter's bone marrow biopsy to look like, if we ever had to diagnose her for leukemia, she changed to 3 hours, and now the bone marrows look fine. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 478073 -- From: Pitts, Jaclyn S. (Jackie), HT(ASCP) pitts.jac...@mayo.edu Sent: Tuesday, March 22, 2011 9:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] B5 Hey all, I was just curious how many of you out there still use B5 as a fixative for bone marrows. Thank Jaclyn Pitts, HT(ASCP) Histotechnician Department of Laboratory Medicine and Pathology Mayo Clinic Rochester, MN E-mail: pitts.jac...@mayo.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: chemicals that are made in the lab
Michele, I would use the expiration dates from the original alcohol bottles for graded alcohols. Alcohol won't expire any faster just because you dilute it. I use two years from the month of recycling for an expiration date for recycled product. In reality these chemicals won't expire for decades as long as they're not contaminated, but a state inspector will want to see reasonable numbers, and I know we will use the chemicals long before the two year mark hits. Nathan Jentsch BS HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
