[Histonet] EDTA decalcification of bones which have been fixed in 70% ethanol
Dear Histonetters, We would like to decalcify some mouse bones in EDTA pH 7 which have been received by our lab already fixed directly in 70% ethanol rather than in formalin or paraformaldehyde. How would you recommend preparing these bones for decalcification e.g. whether to post-fix in formalin or to wash out the ethanol before transferrring to EDTA? I realise that ethanol is not the best fixative to use, especially as the end user may want to do immunocytochemistry or enzyme histochemistry using TRAP. Thanks, Orla -- ** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0114-2713337 (office) 0114-2713174 (lab) E-Mail:o.m.gallag...@sheffield.ac.uk Please think about the environment before printing this email ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] PAX-2
Want to purchase Pax-2 for FFPE human tissue. I have been told that there is a countrywide issue that has it on BACKORDER - I have been waiting for about a 3 to 4 weeks. I have been told that a release date may come in a couple of months. I. Does anyone know enough to confirm this? II. Where do you get yours? Dana Settembre Immunohistochemistry Lab University Hospital - UMDNJ Newark, NJ 07103 USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Alcohol/Xylene resistant ink stamp
Histonetters: I'm looking for a hand stamp to use on standard histo glass slides with ink that is alcohol and xylene resistant. The stamp would just need a few lines (company name). Has anyone ever had one of these made? Thanks for any input, Pam Pamela A. Plumlee H.T. (ASCP) bioTheranostics,Inc. pam.plum...@biotheranostics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Protcol for handling dementia brain biopsies
Hello, We are updating our CJD policy and the question of how to handle brain biopsies with a clinical diagnosis of dementia came up. These are biopsies where CJD is not known or suspected. We are considering if we should follow CJD precautions and protocols for these biopsies. I would appreciate any feedback from those of you handling this kind of specimen. Thanks, Audrey Pagan Lab Manager NYU Langone Medical Center New York, NY /PRE html body br / This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.br / = /body /html PRE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Vantage
We are looking at the Vantage system using it with our CoPath system. Please if you have some time to chat please give me a call or email. I would really appreciate your help and advice. Thanks Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] H E stainer
Does anyone out there have a Surgipath Tribune stainer? Thanks Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lbla...@digestivespecialists.commailto:lbla...@digestivespecialists.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Protcol for handling dementia brain biopsies
Audrey, Our policy is to treat brain biopsies for undiagnosed dementia as if it were CJD. I don't know if I've been lucky or what, but I have only dealt with one casein 30 years. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pagan, Audrey Sent: Wednesday, June 22, 2011 9:10 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Protcol for handling dementia brain biopsies Hello, We are updating our CJD policy and the question of how to handle brain biopsies with a clinical diagnosis of dementia came up. These are biopsies where CJD is not known or suspected. We are considering if we should follow CJD precautions and protocols for these biopsies. I would appreciate any feedback from those of you handling this kind of specimen. Thanks, Audrey Pagan Lab Manager NYU Langone Medical Center New York, NY /PRE html body br / This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.br / = /body /html PRE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HT/HTL position Scottsdale, AZ
New GI lab in Scottsdale, AZ needs an HT/HTL with experience. Beautiful space with lots of windows! Opening mid-July. Very flexible schedule. Can be part-time or fulltime. Interested parties can contact Konni Black via e-mail kbl...@digestivehlth.com or phone 253-503-2560. Thank you, Konni ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job opening in Columbus, OH
Hello Histo-land, We are a small biotech company seeking a part-time HT or HTL for our laboratory. This would be ideal for someone with a full-time job looking for additional work hours. As our work load fluctuates, you must be flexible and willing to go with the flow. Experience in embedding, microtomy, and staining is required, but most of the work will be focused on microtomy, so a high proficiency in this is important. Please send resumes to mol...@phylogenyinc.com Thanks! Adrienne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] EDTA decalcification of bones which have been fixed in 70% ethanol
Since EDTA is essentially an aqueous solution, I would wash out the 70EthOL, fix in NBF for 24 hours and then place the specimen in EDTA until decalcification is completed. René J. --- On Wed, 6/22/11, Orla M Gallagher o.m.gallag...@sheffield.ac.uk wrote: From: Orla M Gallagher o.m.gallag...@sheffield.ac.uk Subject: [Histonet] EDTA decalcification of bones which have been fixed in 70% ethanol To: histonet@lists.utsouthwestern.edu Date: Wednesday, June 22, 2011, 7:55 AM Dear Histonetters, We would like to decalcify some mouse bones in EDTA pH 7 which have been received by our lab already fixed directly in 70% ethanol rather than in formalin or paraformaldehyde. How would you recommend preparing these bones for decalcification e.g. whether to post-fix in formalin or to wash out the ethanol before transferrring to EDTA? I realise that ethanol is not the best fixative to use, especially as the end user may want to do immunocytochemistry or enzyme histochemistry using TRAP. Thanks, Orla -- ** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0114-2713337 (office) 0114-2713174 (lab) E-Mail: o.m.gallag...@sheffield.ac.uk Please think about the environment before printing this email ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Protcol for handling dementia brain biopsies
Dementia per se, as you wrote, is in no way associated with CJD. It is just a stage/type of Alzheimer's disease in no way caused by a highly contagious prion as in the case of CJD. IF they are from a known patien with CJD then you have to follow adequate precautions but in the case of behavioural dementia in an knwon Alzheimer's patient. René J. --- On Wed, 6/22/11, Pagan, Audrey audrey.pa...@nyumc.org wrote: From: Pagan, Audrey audrey.pa...@nyumc.org Subject: [Histonet] Protcol for handling dementia brain biopsies To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Date: Wednesday, June 22, 2011, 10:09 AM Hello, We are updating our CJD policy and the question of how to handle brain biopsies with a clinical diagnosis of dementia came up. These are biopsies where CJD is not known or suspected. We are considering if we should follow CJD precautions and protocols for these biopsies. I would appreciate any feedback from those of you handling this kind of specimen. Thanks, Audrey Pagan Lab Manager NYU Langone Medical Center New York, NY /PRE html body br / This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.br / = /body /html PRE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] need a counter stain for esterase and silver stained slides
I have recently stained some muscle tissue with bromoindigo and urea-silver to examine the neuromuscular junction. The nerve axons and endplates stain quite well this way, but I'd like to add some color to the slide for picture-purposes. Is there a good counterstain (eosin, or Biebrich scarlet-acid fuchsin) I can dip the slides in to add color, WITHOUT taking away from the stain already present in the slides? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] need a counter stain for esterase and silver stained slides
Why don't you try immersing the sections for 1-2 seconds in saturated aqueous picric acid? René J. --- On Wed, 6/22/11, Nicole Cosenza ncose...@siumed.edu wrote: From: Nicole Cosenza ncose...@siumed.edu Subject: [Histonet] need a counter stain for esterase and silver stained slides To: histonet@lists.utsouthwestern.edu Date: Wednesday, June 22, 2011, 3:05 PM I have recently stained some muscle tissue with bromoindigo and urea-silver to examine the neuromuscular junction. The nerve axons and endplates stain quite well this way, but I'd like to add some color to the slide for picture-purposes. Is there a good counterstain (eosin, or Biebrich scarlet-acid fuchsin) I can dip the slides in to add color, WITHOUT taking away from the stain already present in the slides? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formalin Fixation.
Dear all Does Formalin fixation of tissues kill bacteria viruses if present in the tissue? Would you please email any references on the effect of Formalin fixation on the stability of microbes (Viruses, Bacteria Parasites etc) in the tissues? Grateful Thanks Smitha. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Formalin Fixation.
The first uses of formalin by Blum in the late XIX century were to determine its usefulness as a bactericide and, yes, it kills bacteria but not all viruses and no prions. For references search the web. René J. --- On Wed, 6/22/11, smitha rayadurg vjs...@yahoo.com wrote: From: smitha rayadurg vjs...@yahoo.com Subject: [Histonet] Formalin Fixation. To: histonet@lists.utsouthwestern.edu Date: Wednesday, June 22, 2011, 4:13 PM Dear all Does Formalin fixation of tissues kill bacteria viruses if present in the tissue? Would you please email any references on the effect of Formalin fixation on the stability of microbes (Viruses, Bacteria Parasites etc) in the tissues? Grateful Thanks Smitha. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formalin Fixation.
Formalin fixation kills most but not all bacteria. A significant percentage (~10%) of the mycobacteria present in the tissue remain viable. Tuberculosis bacteria have been cultured from ffpe blocks. Prions (Creutzfelt-Jacob disease) are believed to survive formalin fixation. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of smitha rayadurg Sent: Wednesday, June 22, 2011 4:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin Fixation. Dear all Does Formalin fixation of tissues kill bacteria viruses if present in the tissue? Would you please email any references on the effect of Formalin fixation on the stability of microbes (Viruses, Bacteria Parasites etc) in the tissues? Grateful Thanks Smitha. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formalin Fixation.
The reference is K.F. Gerston et al: Viability of mycobacteria in formalin-fixed tissues. International Journal of Tuberculosis and Lung Diseases. 2:521-523 (1998). -Allen A. Smith, Ph.D. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of smitha rayadurg Sent: Wednesday, June 22, 2011 4:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin Fixation. Dear all Does Formalin fixation of tissues kill bacteria viruses if present in the tissue? Would you please email any references on the effect of Formalin fixation on the stability of microbes (Viruses, Bacteria Parasites etc) in the tissues? Grateful Thanks Smitha. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
