Re: [Histonet] (no subject)
Ruthie, In order to unsubscribe from the list, you must click on the link at the bottom of the email, scroll to the bottom of the webpage and enter your email requesting to be unsubscribed. You have the power to unsubscribe yourself! Good Luck! Michelle Sent from my iPhone On Jul 26, 2011, at 12:50 AM, Ruthie Wilson wilsonj...@yahoo.com wrote: Please unsubscribe me ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formalin down the drain??
Ditto Rene! All Federal Regulations trump state if they are more stringent. In other words states can have more stringent regulations but NEVER less. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net Rene J Buesa rjbu...@yahoo.com Sent by: histonet-boun...@lists.utsouthwestern.edu 07/25/2011 04:54 PM To mtitf...@aol.com, histonet@lists.utsouthwestern.edu, BillO'Donnell billodonn...@catholichealth.net cc Subject RE: [Histonet] Formalin down the drain?? Your rant is interesting but wrong. OSHA (which is a FEDERAL agency) prohibits dumping ANY type of hazardous materials down the drain. I was also taken aback by Amy's posting. No, regardless of what your state law may or may not permit you to dump in the drain, you should not put some $avings over the well being of the environment and the drinking water of people. Formaldehyde is toxic and recently officially declared carcinogen. In the same way that frackting methods to obtain gas from shale has been deemed dangerous, equally dumping formaldehyde, xylene and any other chemical ought to be the source of concern. This in my rant! René J. --- On Mon, 7/25/11, O'Donnell, Bill billodonn...@catholichealth.net wrote: From: O'Donnell, Bill billodonn...@catholichealth.net Subject: RE: [Histonet] Formalin down the drain?? To: mtitf...@aol.com, histonet@lists.utsouthwestern.edu Date: Monday, July 25, 2011, 4:19 PM One should not automtically assume that laws are broken here. (Rant begins here) First of all, it is the States that set the limits of what can and cannot be dumped. All States must meet Federal standards,but States are free to determine how they do that. (It's one of the benefits of the American Revolution) Some states are more heavily regulated than others. California and Colorado come to mind immediately. Different organizations, locations and circumstances may allow for disposal of products that may be diluted to such a degree as to be negligable in the waste stream. Our institution generates 65,000 gallons of waste water daily, which allows us to make the dilution limits of anything that our histo lab could produce in a day. No laws are broken if I should pour xylene, formalin, alcohols or other common compounds that we might generate on even our busiest days into the waste stream. HOWEVER, while we may be allowed to do so by state and local regulations, we have decided it is not prudent to do so and so we collect, ship, neutralize or recycle most all that the histo lab generates. We do this at the lab level, with lab funding. It is the responsible thing to do, and we are morally and ethically bound to do so, but we are not outside the law if we do not. If your local municipal waste systems people give you the green light on dumping formalin down the drain. you are not breaking the law, federal or otherwise, in doing so. It is true that if you wish to affect things globally, one has to be responsible locally. Here is what my rant comes down to Make certain that you are meeting local standards for your chemical disposal or you may well be breaking the law. And a big thank you (from myself, my children, grandchildren and great-grand children and that lady who sells me the slurpee at the local convenience store) for anything anyone is doing above and beyond that. :)Rant is over... Have a nice day :) You cannot Like this rant on Facebook or follow this rant on Twitter. Bill -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mtitf...@aol.com Sent: Monday, July 25, 2011 12:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin down the drain?? I was a little distressed to read the message from Amy in Camp Hill, Pennsylvania declaring she dumps everything (and I mean everything) from her histology lab down the drain. There are a bunch of Federal Laws governing handling and disposal of chemicals used in the histology laboratory and she appears to be breaking several. The wastewater law limits how much formalin you can discard down the sink (and you cannot dilute as you go). The same law forbids disposal of organic solvents like xylene, or solutions containing organic solvents. Local laws in Pennsylvania may be more strict. I recommend to Amy that she purchases a book like, Hazardous materials in the histopathology laboratory by Janet Richard Dapson and read the whole thing cover to cover! Michael Titford Pathology USA Mobile AL USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
[Histonet] Histologist Opening in Naples FL
New Opening with a hospital in Naples Day shift position hours roughly 6am to 2:30pm Prefer HT/HTL (ASCP) Will be cutting, staining Please contact me today for immediate consideration Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 http://us.mc538.mail.yahoo.com/mc/compose?to=br...@prometheushealthcare.com br...@prometheushealthcare.com http://www.prometheushealthcare.com/ www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog http://twitter.com/PrometheusBlog ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Antibody suggestions for dog and cat
Hi Mark - We have had good results with: Vimentin Clone V9 (CM048), Factor VIII (PAb-CP039) and pan-Cytokeratin, Clone Lu-5 (CM043) from Biocare CD3 C7930 from Sigma CD79a Clone HM47/A9 (MS357), Melan A (MS799) from Thermo Scientific - no luck with CD20. CD117 (RB9038) from Thermo Scientific - data sheet states heat retrieval is required, but we get good results without retrieval. I have also found that while Melan A is excellent in canine tissue, S100 (PA1-38584, ThermoSci) works better in feline tissue - more positive hits with higher intensity of specific staining. Best regards, Tresa Goins Veterinary Diagnostic Lab South 19th and Lincoln Bozeman, MT 59718 406-994-6353 - phone 406-994-6344 - fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Monday, July 25, 2011 12:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antibody suggestions for dog and cat Could anyone suggest suituble antibodies for the following markers in dog and cat tissue: Vimentin, Pankeratin, CD3 and CD20, CD18, MelanA, Factor VIII Also could use a suggestion for C-kit in Dog tissue only. thanks Mark ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] blurry tissue
Are you using recycled alcohols? Check your concentrations. Could your cassettes be floating up during processing? It is possible they have air bubbles trapped in the cassette and aren't properly dehydrated. Check and change your staining alcohols and clearants to make sure there isn't water from humidity or carry over. It has always been my experience that recycled dehydrants/clearants are the culprit. But, that being said, we have on occasions had an errant cassettes to float up during processing. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net Carol Bryant cb...@lexclin.com Sent by: histonet-boun...@lists.utsouthwestern.edu 07/25/2011 01:42 PM To histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu cc Subject [Histonet] blurry tissue I would like some thoughts on how to resolve some blurry looking tissue. We have had occasional tissue that looks blurry and not crisp for several weeks now. It is not all the cases only random tissues. The tissue is not on the same tissue processor either. We have 2 processors. The latest cases were a breast, some skins, and a prostate. I am not certain if this is happening on the tissue processor or in the stainer. It has been very humid in our lab so I have started running a dehumidifier in case there is water in the xylene. It is so hit and miss that I am puzzled. Any suggestions would be greatly appreciated. Thank you in advance for your thoughts. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Antibody suggestions for dog and cat
Correction: I just found out that LabVision no longer sells the CD3 catalog number that I listed below; we also use CD3 catalog # RB-360 equally well on all species. Jan Shivers UMN VDL - Original Message - From: Jan Shivers shive...@umn.edu To: Mark Tarango marktara...@gmail.com; histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Sent: Monday, July 25, 2011 1:48 PM Subject: Re: [Histonet] Antibody suggestions for dog and cat Hi Mark, Vimentin - Dako; M0725, clone V9 Pankeratin - Dako; M0821; clone MNF116 CD3 - LabVision; RB-9039; rabbit polyclonal CD20 - LabVision; RB-9013; rabbit polyclonal CD18 (canine) - Leukocyte Antigen Biology Lab (UC-Davis; Dr. Peter Moore); clone CA16.3C10 CD18 (feline) - Leukocyte Antigen Biology Lab (UC-Davis; Dr. Peter Moore); clone FE3.9F2 Melan A - Dako; M7195; clone A103 Factor VIII - Dako; A0082; rabbit polyclonal CD117 - Dako; A4502; rabbit polyclonal (not specific for dogs; tested successfully on cow and horse, also) Let me know if you need information on any other antibodies in question. I species-validate all of my IHC tests (100+). Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: Mark Tarango marktara...@gmail.com To: histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Sent: Monday, July 25, 2011 1:33 PM Subject: [Histonet] Antibody suggestions for dog and cat Could anyone suggest suituble antibodies for the following markers in dog and cat tissue: Vimentin, Pankeratin, CD3 and CD20, CD18, MelanA, Factor VIII Also could use a suggestion for C-kit in Dog tissue only. thanks Mark ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Good Morning All, We do the non-gyn cytology cases in the histology lab and usually I will stain the body fluids separate. Now that our workload has increased staining these specimens separately takes up a lot of time. I was wandering what other labs were doing to prevent cross-contamination highly cellular specimens during processing and staining. I am referring to the CAP question below: CYP.07680 ? Are there procedures to prevent cross-contamination of specimens during processing and staining? Thanks in advance for your help, Amy Amy Self Georgetown Hospital System NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MOHS daily flow...
Hello, This is for those techs that assist in the MOHS lab... What is your daily flow for the work performed in your lab? How are patients usually scheduled? (Are they staggered? All come in at approximately the same time so 1st layers can be taken right away?) How often are you receiving patient specimens? Usually, how many patients per day? Do you gross the tissue before freezing? What is the goal time for slide completion? (From the time the tissue is placed in the cryostat to freeze to the slide being ready to stain.) How many levels are you placing on the slide? How many slides per block are you cutting? Do you have a set time limit on when all first layers are to be done? Any additional information is greatly appreciated. Thanks much!! Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor Pathology Department Mayo Clinic Health System in Eau Claire E-mail: bauer.ka...@mayo.edu mailto:bauer.ka...@mayo.edu ___ Mayo Clinic Health System 1221 Whipple St. Eau Claire, WI 54703 www.mayoclinichealthsystem.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] How hot is too hot for storing tissue blocks?
80ºF the most. Use a thermometer and check the temperature weekly. René J. --- On Mon, 7/25/11, Hugh Luk hlu...@msn.com wrote: From: Hugh Luk hlu...@msn.com Subject: [Histonet] How hot is too hot for storing tissue blocks? To: histonet@lists.utsouthwestern.edu Date: Monday, July 25, 2011, 10:53 PM Hi folks, We were hoping someone could recommend a temperature range acceptable for storing tissue blocks? Also do you folks use a regular thermometer or a temperature chart recorder (records on graph) to record room temps? We are going to move to a new location and need help with the fine details. Thanks in advance, Hugh Hawaii ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formalin down the drain??
Not just OSHA, but the EPA prohibits it http://www.epa.gov/lawsregs/topics/waste.html#hazwaste Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, July 25, 2011 4:54 PM To: mtitf...@aol.com; histonet@lists.utsouthwestern.edu; BillO'Donnell Subject: RE: [Histonet] Formalin down the drain?? Your rant is interesting but wrong. OSHA (which is a FEDERAL agency) prohibits dumping ANY type of hazardous materials down the drain. I was also taken aback by Amy's posting. No, regardless of what your state law may or may not permit you to dump in the drain, you should not put some $avings over the well being of the environment and the drinking water of people. Formaldehyde is toxic and recently officially declared carcinogen. In the same way that frackting methods to obtain gas from shale has been deemed dangerous, equally dumping formaldehyde, xylene and any other chemical ought to be the source of concern. This in my rant! René J. --- On Mon, 7/25/11, O'Donnell, Bill billodonn...@catholichealth.net wrote: From: O'Donnell, Bill billodonn...@catholichealth.net Subject: RE: [Histonet] Formalin down the drain?? To: mtitf...@aol.com, histonet@lists.utsouthwestern.edu Date: Monday, July 25, 2011, 4:19 PM One should not automtically assume that laws are broken here. (Rant begins here) First of all, it is the States that set the limits of what can and cannot be dumped. All States must meet Federal standards,but States are free to determine how they do that. (It's one of the benefits of the American Revolution) Some states are more heavily regulated than others. California and Colorado come to mind immediately. Different organizations, locations and circumstances may allow for disposal of products that may be diluted to such a degree as to be negligable in the waste stream. Our institution generates 65,000 gallons of waste water daily, which allows us to make the dilution limits of anything that our histo lab could produce in a day. No laws are broken if I should pour xylene, formalin, alcohols or other common compounds that we might generate on even our busiest days into the waste stream. HOWEVER, while we may be allowed to do so by state and local regulations, we have decided it is not prudent to do so and so we collect, ship, neutralize or recycle most all that the histo lab generates. We do this at the lab level, with lab funding. It is the responsible thing to do, and we are morally and ethically bound to do so, but we are not outside the law if we do not. If your local municipal waste systems people give you the green light on dumping formalin down the drain. you are not breaking the law, federal or otherwise, in doing so. It is true that if you wish to affect things globally, one has to be responsible locally. Here is what my rant comes down to Make certain that you are meeting local standards for your chemical disposal or you may well be breaking the law. And a big thank you (from myself, my children, grandchildren and great-grand children and that lady who sells me the slurpee at the local convenience store) for anything anyone is doing above and beyond that. :)Rant is over... Have a nice day :) You cannot Like this rant on Facebook or follow this rant on Twitter. Bill -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mtitf...@aol.com Sent: Monday, July 25, 2011 12:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin down the drain?? I was a little distressed to read the message from Amy in Camp Hill, Pennsylvania declaring she dumps everything (and I mean everything) from her histology lab down the drain. There are a bunch of Federal Laws governing handling and disposal of chemicals used in the histology laboratory and she appears to be breaking several. The wastewater law limits how much formalin you can discard down the sink (and you cannot dilute as you go). The same law forbids disposal of organic solvents like xylene, or solutions containing organic solvents. Local laws in Pennsylvania may be more strict. I recommend to Amy that she purchases a book like, Hazardous materials in the histopathology laboratory by Janet Richard Dapson and read the whole thing cover to cover! Michael Titford Pathology USA Mobile AL USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
[Histonet] Re: Where Can I Buy Pneumo Control Slides
At the risk of sounding ignorant - easier and easier to do now that I'm 72 - what's Pneumo? Pneumocystis? Pneumococcus? Legionella pneumophila? When you post on Histonet, remember that abbreviations, acronyms, lab slang, and trade names will often not be understood. Many of our readers are not native in English, and many come from other disciplines. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] LR White
Is anyone out there using LR White for routine resin embedding, sectioning, and staining? I am interested in learning some tips for mounting sections on to the slide as wrinkle free as possible. Also our HE stains are a little bit pale. Thanks in advance! Teri ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Pneumocystis Control Slides
I purched a set of 10 from Scientific Device Laboratory so that I could use them to validate an automated GMS stain for pneumo. They were better than nothing, but I wouldn't recommend them only because the quality of material on the slides were not consistent. Some were very thick with lots of background material. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC Histotechnology Technical Specialist West Virginia University Hospitals 304-293-6014 - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Digital pathology systems
At a past NSH S/C, there was a vendor demonstrating digital pathology from the grossing station thru the slide. This was a system for photography of the gross specimen. Can someone remind me what company that was? I believe it was attached to a Thermo workstation. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thanks for Control Offers and Suggestions!!!
Thanks to everyone for your offers and suggestions. I am having a block sent from the control bank at NSH. Thanks again to all, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Antibody suggestions for dog and cat
Mark, Biocare Medical has the following antibodies you are looking for that work on canine tissue (feline not tested): Vimentin - Mouse, clone V9 (CM048) Vimentin - Rabbit, clone SP20 (CRM312) CD3 T-cell - Mouse, clone PS1 (CM110) Mart-1 Cocktail (MelanA) - Mouse, clone M2-7C10+M2-9E3 (CM077) Factor VIII - Rabbit polyclonal (CP039) CD117/c-kit - Rabbit, clone Y145 (CME296) These antibodies work on both canine and feline tissue: Pan Cytokeratin, Mouse, clone AE1/AE3 (CM011) Pan Cytokeratin, Mouse, clone Lu-5 (CM043) Pan Cytokeratin Plus - Mouse, clone AE1/AE3 + 5D3(CM162) CD3 - Rabbit polyclonal (CP215) Feel free to contact me directly if you would like more information or call our Customer Service at 800-799-9499 Opt. #1 Thank you, Brenda Brenda Karim, B.S., M.B.A. Associate Product Manager Biocare Medical, LLC. 4040 Pike Lane, Concord, CA 94520 www.biocare.net tel: 925-603-8072 cell: 925-768-9902 fax: 925-887-8777 email: bka...@biocare.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] MOHS daily flow...
Karen The number of patients depends on the surgeon. Some do 4-15 per day. It also depends on how many rooms you have to see patients. For the most part, Lets say you had two rooms available. and had 6 patients scheduled. schedule pt 15-20 mimutes apart. Bring first two in at same time. Doctor can get layers bandage pateints and place them in a waiting area. Next two patients come in get prepared and layers are taken. Place in waiting area. Next patients are brought in. layers taken. Place in waiting area. Each specmen after layer is taken is brought in lab. Mohs tech should be able to complete each case within 15-20minutes. Slides are placed for doc to read. Once he has taken all layers he can begin to read ready cases and take layers or begin closures. Pateints layers are taken and then they are placed back in waiting areas. This will continue until are patients are done. Tissue is grossed before it is placed in cryo. Two slides should be cut per peice of tissue. Not per case. We place 3 sections on each slide. Each section is a little deeper then the one before. We start at the frosted end. A great fast effecient tech should be able to handle 10-15 cases per day. I hope this helps. Nicole Tatum HT ASCP This is for those techs that assist in the MOHS lab... What is your daily flow for the work performed in your lab? How are patients usually scheduled? (Are they staggered? All come in at approximately the same time so 1st layers can be taken right away?) How often are you receiving patient specimens? Usually, how many patients per day? Do you gross the tissue before freezing? What is the goal time for slide completion? (From the time the tissue is placed in the cryostat to freeze to the slide being ready to stain.) How many levels are you placing on the slide? How many slides per block are you cutting? Do you have a set time limit on when all first layers are to be done? Any additional information is greatly appreciated. Thanks much!! Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor Pathology Department Mayo Clinic Health System in Eau Claire E-mail: bauer.ka...@mayo.edu mailto:bauer.ka...@mayo.edu ___ Mayo Clinic Health System 1221 Whipple St. Eau Claire, WI 54703 www.mayoclinichealthsystem.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 92, Issue 35
Rrr Tina (Phan) McLaughlin SYMPHONY Product Specialist Ventana Medical Systems, a Division of Roche Cell: 520.230.0175 www.ventanamed.com Sent by Verizon Blackberry - Original Message - From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tue Jul 26 19:02:28 2011 Subject: Histonet Digest, Vol 92, Issue 35 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. LR White (Johnson, Teri) 2. Re: Where Can I Buy Pneumo Control Slides (Bob Richmond) 3. Re: Pneumocystis Control Slides (Oneil, Beth Ann) 4. Digital pathology systems (Breeden, Sara) -- Message: 1 Date: Tue, 26 Jul 2011 10:29:06 -0500 From: Johnson, Teri t...@stowers.org Subject: [Histonet] LR White To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: 2c40e43d1f7a56408c4463fd245dddf977056...@exchmb-02.stowers-institute.org Content-Type: text/plain; charset=us-ascii Is anyone out there using LR White for routine resin embedding, sectioning, and staining? I am interested in learning some tips for mounting sections on to the slide as wrinkle free as possible. Also our HE stains are a little bit pale. Thanks in advance! Teri -- Message: 2 Date: Tue, 26 Jul 2011 11:30:59 -0400 From: Bob Richmond rsrichm...@gmail.com Subject: [Histonet] Re: Where Can I Buy Pneumo Control Slides To: histonet@lists.utsouthwestern.edu Message-ID: CAOKsRH7z6Svr3tedtc-RFGpZRp8Nvmr4WWxREWMHtd=tnhn...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 At the risk of sounding ignorant - easier and easier to do now that I'm 72 - what's Pneumo? Pneumocystis? Pneumococcus? Legionella pneumophila? When you post on Histonet, remember that abbreviations, acronyms, lab slang, and trade names will often not be understood. Many of our readers are not native in English, and many come from other disciplines. Bob Richmond Samurai Pathologist Knoxville TN -- Message: 3 Date: Tue, 26 Jul 2011 11:35:55 -0400 From: Oneil, Beth Ann one...@wvuhealthcare.com Subject: [Histonet] Re: Pneumocystis Control Slides To: histonet@lists.utsouthwestern.edu Message-ID: 052c41417e0ef64d8e201fe822d36ca90a1c5...@nt-exchange.wvuh.wvuhs.com Content-Type: text/plain; charset=iso-8859-1 I purched a set of 10 from Scientific Device Laboratory so that I could use them to validate an automated GMS stain for pneumo. They were better than nothing, but I wouldn't recommend them only because the quality of material on the slides were not consistent. Some were very thick with lots of background material. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC Histotechnology Technical Specialist West Virginia University Hospitals 304-293-6014 - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. -- Message: 4 Date: Tue, 26 Jul 2011 10:27:24 -0600 From: Breeden, Sara sbree...@nmda.nmsu.edu Subject: [Histonet] Digital pathology systems To: Histonet@lists.utsouthwestern.edu Message-ID: 4d14f0fc9316dd41972d5f03c070908b051df...@nmdamailsvr.nmda.ad.nmsu.edu Content-Type: text/plain; charset=us-ascii At a past NSH S/C, there was a vendor demonstrating digital pathology from the grossing station thru the slide. This was a system for photography of the gross specimen. Can someone remind me what company that was? I believe it was attached to a Thermo workstation. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 92, Issue 35 ___ Histonet
[Histonet] Re: Histonet Digest, Vol 92, Issue 35
Trtttrt Tina (Phan) McLaughlin SYMPHONY Product Specialist Ventana Medical Systems, a Division of Roche Cell: 520.230.0175 www.ventanamed.com Sent by Verizon Blackberry - Original Message - From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tue Jul 26 19:02:28 2011 Subject: Histonet Digest, Vol 92, Issue 35 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. LR White (Johnson, Teri) 2. Re: Where Can I Buy Pneumo Control Slides (Bob Richmond) 3. Re: Pneumocystis Control Slides (Oneil, Beth Ann) 4. Digital pathology systems (Breeden, Sara) -- Message: 1 Date: Tue, 26 Jul 2011 10:29:06 -0500 From: Johnson, Teri t...@stowers.org Subject: [Histonet] LR White To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: 2c40e43d1f7a56408c4463fd245dddf977056...@exchmb-02.stowers-institute.org Content-Type: text/plain; charset=us-ascii Is anyone out there using LR White for routine resin embedding, sectioning, and staining? I am interested in learning some tips for mounting sections on to the slide as wrinkle free as possible. Also our HE stains are a little bit pale. Thanks in advance! Teri -- Message: 2 Date: Tue, 26 Jul 2011 11:30:59 -0400 From: Bob Richmond rsrichm...@gmail.com Subject: [Histonet] Re: Where Can I Buy Pneumo Control Slides To: histonet@lists.utsouthwestern.edu Message-ID: CAOKsRH7z6Svr3tedtc-RFGpZRp8Nvmr4WWxREWMHtd=tnhn...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 At the risk of sounding ignorant - easier and easier to do now that I'm 72 - what's Pneumo? Pneumocystis? Pneumococcus? Legionella pneumophila? When you post on Histonet, remember that abbreviations, acronyms, lab slang, and trade names will often not be understood. Many of our readers are not native in English, and many come from other disciplines. Bob Richmond Samurai Pathologist Knoxville TN -- Message: 3 Date: Tue, 26 Jul 2011 11:35:55 -0400 From: Oneil, Beth Ann one...@wvuhealthcare.com Subject: [Histonet] Re: Pneumocystis Control Slides To: histonet@lists.utsouthwestern.edu Message-ID: 052c41417e0ef64d8e201fe822d36ca90a1c5...@nt-exchange.wvuh.wvuhs.com Content-Type: text/plain; charset=iso-8859-1 I purched a set of 10 from Scientific Device Laboratory so that I could use them to validate an automated GMS stain for pneumo. They were better than nothing, but I wouldn't recommend them only because the quality of material on the slides were not consistent. Some were very thick with lots of background material. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC Histotechnology Technical Specialist West Virginia University Hospitals 304-293-6014 - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. -- Message: 4 Date: Tue, 26 Jul 2011 10:27:24 -0600 From: Breeden, Sara sbree...@nmda.nmsu.edu Subject: [Histonet] Digital pathology systems To: Histonet@lists.utsouthwestern.edu Message-ID: 4d14f0fc9316dd41972d5f03c070908b051df...@nmdamailsvr.nmda.ad.nmsu.edu Content-Type: text/plain; charset=us-ascii At a past NSH S/C, there was a vendor demonstrating digital pathology from the grossing station thru the slide. This was a system for photography of the gross specimen. Can someone remind me what company that was? I believe it was attached to a Thermo workstation. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 92, Issue 35
RE: [Histonet] MOHS daily flow...
That is very interesting. A friend had a procedure done last week and they kept him on the table until they were done. he did not go back to a waiting area. Is this common? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum Sent: Tuesday, July 26, 2011 1:40 PM To: Bauer, Karen L.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] MOHS daily flow... Importance: Low Karen The number of patients depends on the surgeon. Some do 4-15 per day. It also depends on how many rooms you have to see patients. For the most part, Lets say you had two rooms available. and had 6 patients scheduled. schedule pt 15-20 mimutes apart. Bring first two in at same time. Doctor can get layers bandage pateints and place them in a waiting area. Next two patients come in get prepared and layers are taken. Place in waiting area. Next patients are brought in. layers taken. Place in waiting area. Each specmen after layer is taken is brought in lab. Mohs tech should be able to complete each case within 15-20minutes. Slides are placed for doc to read. Once he has taken all layers he can begin to read ready cases and take layers or begin closures. Pateints layers are taken and then they are placed back in waiting areas. This will continue until are patients are done. Tissue is grossed before it is placed in cryo. Two slides should be cut per peice of tissue. Not per case. We place 3 sections on each slide. Each section is a little deeper then the one before. We start at the frosted end. A great fast effecient tech should be able to handle 10-15 cases per day. I hope this helps. Nicole Tatum HT ASCP This is for those techs that assist in the MOHS lab... What is your daily flow for the work performed in your lab? How are patients usually scheduled? (Are they staggered? All come in at approximately the same time so 1st layers can be taken right away?) How often are you receiving patient specimens? Usually, how many patients per day? Do you gross the tissue before freezing? What is the goal time for slide completion? (From the time the tissue is placed in the cryostat to freeze to the slide being ready to stain.) How many levels are you placing on the slide? How many slides per block are you cutting? Do you have a set time limit on when all first layers are to be done? Any additional information is greatly appreciated. Thanks much!! Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor Pathology Department Mayo Clinic Health System in Eau Claire E-mail: bauer.ka...@mayo.edu mailto:bauer.ka...@mayo.edu ___ Mayo Clinic Health System 1221 Whipple St. Eau Claire, WI 54703 www.mayoclinichealthsystem.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 92, Issue 35
R Tina (Phan) McLaughlin SYMPHONY Product Specialist Ventana Medical Systems, a Division of Roche Cell: 520.230.0175 www.ventanamed.com Sent by Verizon Blackberry - Original Message - From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tue Jul 26 19:02:28 2011 Subject: Histonet Digest, Vol 92, Issue 35 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. LR White (Johnson, Teri) 2. Re: Where Can I Buy Pneumo Control Slides (Bob Richmond) 3. Re: Pneumocystis Control Slides (Oneil, Beth Ann) 4. Digital pathology systems (Breeden, Sara) -- Message: 1 Date: Tue, 26 Jul 2011 10:29:06 -0500 From: Johnson, Teri t...@stowers.org Subject: [Histonet] LR White To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: 2c40e43d1f7a56408c4463fd245dddf977056...@exchmb-02.stowers-institute.org Content-Type: text/plain; charset=us-ascii Is anyone out there using LR White for routine resin embedding, sectioning, and staining? I am interested in learning some tips for mounting sections on to the slide as wrinkle free as possible. Also our HE stains are a little bit pale. Thanks in advance! Teri -- Message: 2 Date: Tue, 26 Jul 2011 11:30:59 -0400 From: Bob Richmond rsrichm...@gmail.com Subject: [Histonet] Re: Where Can I Buy Pneumo Control Slides To: histonet@lists.utsouthwestern.edu Message-ID: CAOKsRH7z6Svr3tedtc-RFGpZRp8Nvmr4WWxREWMHtd=tnhn...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 At the risk of sounding ignorant - easier and easier to do now that I'm 72 - what's Pneumo? Pneumocystis? Pneumococcus? Legionella pneumophila? When you post on Histonet, remember that abbreviations, acronyms, lab slang, and trade names will often not be understood. Many of our readers are not native in English, and many come from other disciplines. Bob Richmond Samurai Pathologist Knoxville TN -- Message: 3 Date: Tue, 26 Jul 2011 11:35:55 -0400 From: Oneil, Beth Ann one...@wvuhealthcare.com Subject: [Histonet] Re: Pneumocystis Control Slides To: histonet@lists.utsouthwestern.edu Message-ID: 052c41417e0ef64d8e201fe822d36ca90a1c5...@nt-exchange.wvuh.wvuhs.com Content-Type: text/plain; charset=iso-8859-1 I purched a set of 10 from Scientific Device Laboratory so that I could use them to validate an automated GMS stain for pneumo. They were better than nothing, but I wouldn't recommend them only because the quality of material on the slides were not consistent. Some were very thick with lots of background material. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC Histotechnology Technical Specialist West Virginia University Hospitals 304-293-6014 - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. -- Message: 4 Date: Tue, 26 Jul 2011 10:27:24 -0600 From: Breeden, Sara sbree...@nmda.nmsu.edu Subject: [Histonet] Digital pathology systems To: Histonet@lists.utsouthwestern.edu Message-ID: 4d14f0fc9316dd41972d5f03c070908b051df...@nmdamailsvr.nmda.ad.nmsu.edu Content-Type: text/plain; charset=us-ascii At a past NSH S/C, there was a vendor demonstrating digital pathology from the grossing station thru the slide. This was a system for photography of the gross specimen. Can someone remind me what company that was? I believe it was attached to a Thermo workstation. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 92, Issue 35 ___ Histonet
[Histonet] CRO for mouse brain paraffin sections
Hi, We are looking for a CRO experienced in step/serial sections of mouse brain and with a short turnaround time. Any recommendation is appreciated. Please contact me directly at shu-cheng.c...@merck.commailto:shu-cheng.c...@merck.com Thanks, Shu-Cheng Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] MOHS daily flow...
Hi Jeanine, Our patients go back to a MOHS only waiting room between layers. Nice comfy loungers, big screen TV, refreshments, things to make them comfortable while they wait, since it could be a long day for them. They can bring in one family member with them if they want. Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor Pathology Department Mayo Clinic Health System in Eau Claire E-mail: bauer.ka...@mayo.edu ___ Mayo Clinic Health System 1221 Whipple St. Eau Claire, WI 54703 www.mayoclinichealthsystem.org -Original Message- From: Bartlett, Jeanine (CDC/OID/NCEZID) [mailto:j...@cdc.gov] Sent: Tuesday, July 26, 2011 12:53 PM To: Nicole Tatum; Bauer, Karen L.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] MOHS daily flow... That is very interesting. A friend had a procedure done last week and they kept him on the table until they were done. he did not go back to a waiting area. Is this common? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum Sent: Tuesday, July 26, 2011 1:40 PM To: Bauer, Karen L.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] MOHS daily flow... Importance: Low Karen The number of patients depends on the surgeon. Some do 4-15 per day. It also depends on how many rooms you have to see patients. For the most part, Lets say you had two rooms available. and had 6 patients scheduled. schedule pt 15-20 mimutes apart. Bring first two in at same time. Doctor can get layers bandage pateints and place them in a waiting area. Next two patients come in get prepared and layers are taken. Place in waiting area. Next patients are brought in. layers taken. Place in waiting area. Each specmen after layer is taken is brought in lab. Mohs tech should be able to complete each case within 15-20minutes. Slides are placed for doc to read. Once he has taken all layers he can begin to read ready cases and take layers or begin closures. Pateints layers are taken and then they are placed back in waiting areas. This will continue until are patients are done. Tissue is grossed before it is placed in cryo. Two slides should be cut per peice of tissue. Not per case. We place 3 sections on each slide. Each section is a little deeper then the one before. We start at the frosted end. A great fast effecient tech should be able to handle 10-15 cases per day. I hope this helps. Nicole Tatum HT ASCP This is for those techs that assist in the MOHS lab... What is your daily flow for the work performed in your lab? How are patients usually scheduled? (Are they staggered? All come in at approximately the same time so 1st layers can be taken right away?) How often are you receiving patient specimens? Usually, how many patients per day? Do you gross the tissue before freezing? What is the goal time for slide completion? (From the time the tissue is placed in the cryostat to freeze to the slide being ready to stain.) How many levels are you placing on the slide? How many slides per block are you cutting? Do you have a set time limit on when all first layers are to be done? Any additional information is greatly appreciated. Thanks much!! Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor Pathology Department Mayo Clinic Health System in Eau Claire E-mail: bauer.ka...@mayo.edu mailto:bauer.ka...@mayo.edu ___ Mayo Clinic Health System 1221 Whipple St. Eau Claire, WI 54703 www.mayoclinichealthsystem.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formalin down the drain??
Been reading all these responses...so funny how different places do different things. An old job I worked at poured gallons and gallons down the drain on a daily basis. I do think there was some kind of filter system in place, but all the same...how do you filter out all the little things?? I would say just follow whatever your EHS people say =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Tuesday, July 26, 2011 10:15 AM To: Rene J Buesa; mtitf...@aol.com; histonet@lists.utsouthwestern.edu; BillO'Donnell Subject: RE: [Histonet] Formalin down the drain?? Not just OSHA, but the EPA prohibits it http://www.epa.gov/lawsregs/topics/waste.html#hazwaste Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, July 25, 2011 4:54 PM To: mtitf...@aol.com; histonet@lists.utsouthwestern.edu; BillO'Donnell Subject: RE: [Histonet] Formalin down the drain?? Your rant is interesting but wrong. OSHA (which is a FEDERAL agency) prohibits dumping ANY type of hazardous materials down the drain. I was also taken aback by Amy's posting. No, regardless of what your state law may or may not permit you to dump in the drain, you should not put some $avings over the well being of the environment and the drinking water of people. Formaldehyde is toxic and recently officially declared carcinogen. In the same way that frackting methods to obtain gas from shale has been deemed dangerous, equally dumping formaldehyde, xylene and any other chemical ought to be the source of concern. This in my rant! René J. --- On Mon, 7/25/11, O'Donnell, Bill billodonn...@catholichealth.net wrote: From: O'Donnell, Bill billodonn...@catholichealth.net Subject: RE: [Histonet] Formalin down the drain?? To: mtitf...@aol.com, histonet@lists.utsouthwestern.edu Date: Monday, July 25, 2011, 4:19 PM One should not automtically assume that laws are broken here. (Rant begins here) First of all, it is the States that set the limits of what can and cannot be dumped. All States must meet Federal standards,but States are free to determine how they do that. (It's one of the benefits of the American Revolution) Some states are more heavily regulated than others. California and Colorado come to mind immediately. Different organizations, locations and circumstances may allow for disposal of products that may be diluted to such a degree as to be negligable in the waste stream. Our institution generates 65,000 gallons of waste water daily, which allows us to make the dilution limits of anything that our histo lab could produce in a day. No laws are broken if I should pour xylene, formalin, alcohols or other common compounds that we might generate on even our busiest days into the waste stream. HOWEVER, while we may be allowed to do so by state and local regulations, we have decided it is not prudent to do so and so we collect, ship, neutralize or recycle most all that the histo lab generates. We do this at the lab level, with lab funding. It is the responsible thing to do, and we are morally and ethically bound to do so, but we are not outside the law if we do not. If your local municipal waste systems people give you the green light on dumping formalin down the drain. you are not breaking the law, federal or otherwise, in doing so. It is true that if you wish to affect things globally, one has to be responsible locally. Here is what my rant comes down to Make certain that you are meeting local standards for your chemical disposal or you may well be breaking the law. And a big thank you (from myself, my children, grandchildren and great-grand children and that lady who sells me the slurpee at the local convenience store) for anything anyone is doing above and beyond that. :)Rant is over... Have a nice day :) You cannot Like this rant on Facebook or follow this rant on Twitter. Bill -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mtitf...@aol.com Sent: Monday, July 25, 2011 12:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin down the drain?? I was a little distressed to read the message from Amy in Camp Hill, Pennsylvania declaring she dumps everything (and I mean everything) from her histology lab down the drain. There are a bunch of Federal Laws governing handling and disposal of chemicals used in the histology laboratory and she appears to be breaking several. The wastewater law limits how much formalin you can discard down the sink (and you cannot
[Histonet] Re: Histonet Digest, Vol 92, Issue 35
My apologies to the members of Histonet! My blackberry had unfortunately dialed by accident. Tina (Phan) McLaughlin SYMPHONY Product Specialist Ventana Medical Systems, a Division of Roche Cell: 520.230.0175 www.ventanamed.com Sent by Verizon Blackberry - Original Message - From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tue Jul 26 19:02:28 2011 Subject: Histonet Digest, Vol 92, Issue 35 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. LR White (Johnson, Teri) 2. Re: Where Can I Buy Pneumo Control Slides (Bob Richmond) 3. Re: Pneumocystis Control Slides (Oneil, Beth Ann) 4. Digital pathology systems (Breeden, Sara) -- Message: 1 Date: Tue, 26 Jul 2011 10:29:06 -0500 From: Johnson, Teri t...@stowers.org Subject: [Histonet] LR White To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: 2c40e43d1f7a56408c4463fd245dddf977056...@exchmb-02.stowers-institute.org Content-Type: text/plain; charset=us-ascii Is anyone out there using LR White for routine resin embedding, sectioning, and staining? I am interested in learning some tips for mounting sections on to the slide as wrinkle free as possible. Also our HE stains are a little bit pale. Thanks in advance! Teri -- Message: 2 Date: Tue, 26 Jul 2011 11:30:59 -0400 From: Bob Richmond rsrichm...@gmail.com Subject: [Histonet] Re: Where Can I Buy Pneumo Control Slides To: histonet@lists.utsouthwestern.edu Message-ID: CAOKsRH7z6Svr3tedtc-RFGpZRp8Nvmr4WWxREWMHtd=tnhn...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 At the risk of sounding ignorant - easier and easier to do now that I'm 72 - what's Pneumo? Pneumocystis? Pneumococcus? Legionella pneumophila? When you post on Histonet, remember that abbreviations, acronyms, lab slang, and trade names will often not be understood. Many of our readers are not native in English, and many come from other disciplines. Bob Richmond Samurai Pathologist Knoxville TN -- Message: 3 Date: Tue, 26 Jul 2011 11:35:55 -0400 From: Oneil, Beth Ann one...@wvuhealthcare.com Subject: [Histonet] Re: Pneumocystis Control Slides To: histonet@lists.utsouthwestern.edu Message-ID: 052c41417e0ef64d8e201fe822d36ca90a1c5...@nt-exchange.wvuh.wvuhs.com Content-Type: text/plain; charset=iso-8859-1 I purched a set of 10 from Scientific Device Laboratory so that I could use them to validate an automated GMS stain for pneumo. They were better than nothing, but I wouldn't recommend them only because the quality of material on the slides were not consistent. Some were very thick with lots of background material. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC Histotechnology Technical Specialist West Virginia University Hospitals 304-293-6014 - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. -- Message: 4 Date: Tue, 26 Jul 2011 10:27:24 -0600 From: Breeden, Sara sbree...@nmda.nmsu.edu Subject: [Histonet] Digital pathology systems To: Histonet@lists.utsouthwestern.edu Message-ID: 4d14f0fc9316dd41972d5f03c070908b051df...@nmdamailsvr.nmda.ad.nmsu.edu Content-Type: text/plain; charset=us-ascii At a past NSH S/C, there was a vendor demonstrating digital pathology from the grossing station thru the slide. This was a system for photography of the gross specimen. Can someone remind me what company that was? I believe it was attached to a Thermo workstation. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 92, Issue 35
RE: [Histonet] Re: Histonet Digest, Vol 92, Issue 35
I had this vision of your Blackberry in your back pocket --- well, you know... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Methasol Fast Blue
We are looking for a vendor for Methasol Fast Blue. AKA Fast blue or Solvent Blue 24 and a protocol for a Modified Maxwell Stain. Some time ago - 2004 - there was a discussion on histonet about this stain. Margaret Blount had some protocols...Margaret if you are still on histonet can you email me and let me know about these protocols? I have some sort of protocol gleaned from a paper. I also can't locate any source that sells the stain. Thanks! Andi Grantham___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] which conferences?
dear all what conference do you recommend in histology and histotechnology feild do you prefer? please provide me with date and a link if avaliable thanx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] which conferences?
NSH!!! Great information...great people...great locations...and great nightly parties =) It's September 16 this year. http://s3.goeshow.com/nsh/annual/2011/index.cfm Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Tuesday, July 26, 2011 4:18 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] which conferences? dear all what conference do you recommend in histology and histotechnology feild do you prefer? please provide me with date and a link if avaliable thanx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] which conferences?
NSH. Nsh.org Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Tuesday, July 26, 2011 2:18 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] which conferences? dear all what conference do you recommend in histology and histotechnology feild do you prefer? please provide me with date and a link if avaliable thanx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] which conferences?
National Society for Histotechnology (Sept 16-21, 2011). www.nsh.org Montina J. Van Meter, HT (ASCP) Lab Manager Dept. of Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Tuesday, July 26, 2011 4:18 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] which conferences? dear all what conference do you recommend in histology and histotechnology feild do you prefer? please provide me with date and a link if avaliable thanx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] which conferences?
What type of histology interests you? Research or Clinical? Soft Tissue (paraffin) or Hard Tissue (resin)? I am giving a teleconference sponsored by the National Society for Histotechnolgy (NSH) next month (August 17th) as part of their VIR Summer Teleconference Series. During this teleconference I will be talking about the use of resin for undemineralized bone histology. Definitely check this out if you have interest in working with undemineralized bone! Resin Histology: A Practical Approach for Demonstrating Undemineralized Bone Presented by Jack Ratliff, BioMimetic Therapeutics, Inc. As musculoskeletal research progresses with new technological advancements in the areas of biological repair and replacement, histological evaluation continues to play a crucial role in the determination of safety and efficacy for these new treatments. While most will employ traditional and acceptable methods of decalcification and paraffin embedding for the demonstration of these critical components of evaluation, these techniques can sometimes be very challenging and/or impossible when presented with a variety of implant materials or devices. For example, to evaluate safety and efficacy of a metallic device coated with a biological therapeutic at the bone interface, one will need to forego traditional methods of decalcification and seek an undisturbed representation of the specimen by utilizing an embedding media that is both as hard as the specimen and the implant material. Additionally, it may also be important to use a media that will not distort or dissolve the coating. This seminar will address the use of resin histology techniques for the demonstration of undemineralized bone. Topics will include tissue preparation, fixation, processing, infiltration, and embedding/polymerization with acrylic resins. We will also discuss two types of microtomy as related to small and large undemineralized bone specimens and the presence or absence of implant materials. Jack Date: Tue, 26 Jul 2011 14:17:45 -0700 From: k8...@yahoo.com To: Histonet@lists.utsouthwestern.edu CC: Subject: [Histonet] which conferences? dear all what conference do you recommend in histology and histotechnology feild do you prefer? please provide me with date and a link if avaliable thanx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] repetitive wrist injury
Has anyone experienced problems with the histotechnologists or Pathologist's Assistants developing wrist injuries from opening the small specimen containers all day on the grossing bench? If so, how did you address this issue? We have had occupational health assess the process; there haven't been any suggestions yet. Cathy Kelowna, B.C. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
