Re: [Histonet] RE: Pro-Par
We do rotate our alcohols after eosin to minimize this problem. We recycle our alcohol from there so there is a cost savings. Every lab has their setup that works best for them while pleasing the pathologist(s) too. I love this forum because you can get informed opinions! On Tue, Feb 7, 2012 at 4:32 PM, Victor A. Tobias vtob...@uw.edu wrote: In a previous job we used Pro-Par and recycled it too for many years. I don't know if I would label it a drawback, but I felt the dehydrating steps after eosin required changing more often then if using xylene. There were days when we would get the dreaded bleeding eosin. Keep your reagents fresh and you shouldn't have any problems. We were hand coverslipping back then. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtob...@u.washington.edu 206-744-2735 206-744-8240 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, February 07, 2012 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pro-Par Hi Folks, Just curious...anyone using Pro-Par care to share an opinion? Have considered it off and on over the years and now wondering what folks think. Also, anyone using it with tape coverslippers? Maybe this has been asked and I wasn't keeping up with the thread. Thanks, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Great Position
Great opportunity for a Histotechnician in a brand new laboratory! Acadiana Gastroenterology Associates, LLC, a six (6) Physician Practice located in Lafayette, Louisiana, is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and CLIA certified to perform gross dissection, prior supervisory experience preferred. Responsibilities would include the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. This is a part-time position that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mh...@carisls.com http://webmail.windstreamhosting.com/hwebmail/services/go.php?url=http% 3A%2F%2Fmailto%3Amhale%40carisls.com NO recruiters please. Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Diagnostics Part of Miraca Holdings Inc. 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Odor of old paraffin blocks
I'm wondering if anyone can help me with this question. I've been in our block/slide storage room organizing blocks from the early 80's and every drawer smells like ripe bananas. I've thought about someone leaving a banana or peel hidden in the room, but would imagine by now any odor would be gone, and honestly...it's kinda a scary room so I'm not looking much past the blocks and slides. Our blocks are not sealed and I'm at a lost for what could be causing the odor. Thanks, Pamm Bakken Childrens Hospital Minneapolis Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Odor of old paraffin blocks
They could have used amyl acetate as a clearing agent for alcohol. The product is a banana oil is very smelly. It was done for years with neuro specimens in some reseach labs and not as oft en (rarely) in clinical Histology. It had to cleared with another organic, usually benezene. One research lab I worked in used it routinely until we changed the protocol completely for safety reasons. It also made me crave banana splits!! Pam Marcum - Original Message - From: Pam Bakken pam.bak...@childrensmn.org To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 8, 2012 8:10:27 AM Subject: [Histonet] Odor of old paraffin blocks I'm wondering if anyone can help me with this question. I've been in our block/slide storage room organizing blocks from the early 80's and every drawer smells like ripe bananas. I've thought about someone leaving a banana or peel hidden in the room, but would imagine by now any odor would be gone, and honestly...it's kinda a scary room so I'm not looking much past the blocks and slides. Our blocks are not sealed and I'm at a lost for what could be causing the odor. Thanks, Pamm Bakken Childrens Hospital Minneapolis Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] VIP 6 tissue processing
Dear Fellow members, I am working in laboratory animal pathology lab and we have recently purchased a Sakura VIP tissue processor. We are working on standardising the tissue processing schedules. We require to process only tissues from rats and mice. My request to the group is if there anyone using this processor for processing of tissues from rodents? If yes is it possible to share with me the protocol which works fine in your lab. We are planning to use paraffin(paraplast general) for both processing and embedding. My second question is whether paraplast general paraffin works well with rodent tissues or we need to think about the paraplast plus? Whether different types of paraffin should be used in embedders and processors or same type of paraffin will be okey? Please free to share your experience to my mail With best regards Murugesh Histotechnician Laboratory animal research Madras veterinary college Chennai India ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Morse fixative/solution
Hi histonetters, Does anybody know, if Morse fixative/solution is commercially available? Thanks in advance, Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2588 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GSH-Region III meeting reminder
Hi Histonetters, I wanted to remind you guys of the Georgia Society for Histotechnology - Region III meeting at Callaway Gardens, Pine Mountain GA April 13-15th,. The Deadline for receiving the discounted hotel rate is March 13th, so act now. Remember The Mountain Creek Inn fills up fast during the spring which is golfing season here in Georgia. Making reservations with a credit card will hold your room, they will not apply the rate until you actually check in. Call now 1-800-225-5292 and use the GSH group # 78K711 to get the discounted room rate of $109. There are other rooming option rates in the program which can be found at http://www.histosearch.com/gsh/symposium.html. Also register early for the fantastic workshops GSH has lined up; earn your CEUs. GSH has planned a great awards luncheon on Saturday as well as a dinner on Saturday night, details are in the program. Payment is easy with PayPal. You do not have to have an account with them to use this service and it is secure. Make payment by going to www.PayPal.comhttp://www.PayPal.com and send payment to the email address on the registration form. Plan to attend, bring the family for a vacation, Callaway Gardens is a wonderful family experience. If you have any questions please contact GSH President - Mike Ayers at lmay...@charter.netmailto:lmay...@charter.net, GSH Vice President and Exhibit Liaison - Wanda Simons at wandr...@att.netmailto:wandr...@att.net or myself powell...@mercer.edumailto:powell...@mercer.edu. Please pass this invitation to any who may not get this email. Come to Georgia and experience Histotechnology - Southern Style Shirley Powell GSH Secretary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cryostats decontamination
Is everyone taking their crostats down weekly?? just seems overkill to me thanks, anita dudley ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Revco -80 Freezer Sliding Drawer Rack Reviews
Hi all, We're going to be purchasing a Revco UxF -86 C upright freezer soon and we'd like to fill it with 2 box racks. Revco offers 2 options: the standard side-access racks and sliding drawer racks. The idea of the sliding drawer racks sounds great (you don't have to pull the entire rack out of the freezer, just a drawer), but I'm wondering if, in practice, they're not as great as they sound. I imagine they could freeze up, be hard to slide, etc. I have yet to find any reviews regarding the racks online, so I thought I'd come to you guys. Does anyone use the sliding drawer racks and have an opinion about them? Should we just stick with the tried and true side-access racks? Thanks for your input! Lucie Lucie Guernsey UC San Diego lguern...@ucsd.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
