Re: [Histonet] Frozen Sections Falling Off Slides
Was your leica set that cold? Maybe you could try warming the slide for a couple seconds before putting in alcohol. As far a charged slides I am amazed with the slides Dako sells for their IHC. Sent from my iPhone On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario wrote: > Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen section > Machine...But i dont understand why i got problems sections falling off > slides.I used different types of slides non adhesive,adhesive(poly l > lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken > from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative > etc..then after distilled water wash out...sections started to peel off...My > machine setting- chamber is -30 C;cryochamber is -39 C..example of tissue- > fibroma and salivary gland... > I used Leica cryostat previously but i never got these problems...Even i used > non adhesive but sections still on the slides..Can someone advised > please.Maybe youre using the same tissue tek cryo 3... > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Frozen Sections Falling Off Slides
Although it should NOT be the cause, I always used my cryostat at -20 to -25ºC Second I always warmed the slides (at least 30ºC) before picking the sections. This requires having a well extended cryosection and to pick them up with a "smooth movement". You will not have any chance of "rearranging-extending" the section. As a fixative I used NBF for 30 seconds → to regular or special staining. René J. From: Bernadette del Rosario To: "Histonet@lists.utsouthwestern.edu" Sent: Saturday, June 16, 2012 12:01 PM Subject: [Histonet] Frozen Sections Falling Off Slides Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen section Machine...But i dont understand why i got problems sections falling off slides.I used different types of slides non adhesive,adhesive(poly l lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative etc..then after distilled water wash out...sections started to peel off...My machine setting- chamber is -30 C;cryochamber is -39 C..example of tissue- fibroma and salivary gland... I used Leica cryostat previously but i never got these problems...Even i used non adhesive but sections still on the slides..Can someone advised please.Maybe youre using the same tissue tek cryo 3... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Frozen Sections Falling Off Slides
Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen section Machine...But i dont understand why i got problems sections falling off slides.I used different types of slides non adhesive,adhesive(poly l lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative etc..then after distilled water wash out...sections started to peel off...My machine setting- chamber is -30 C;cryochamber is -39 C..example of tissue- fibroma and salivary gland... I used Leica cryostat previously but i never got these problems...Even i used non adhesive but sections still on the slides..Can someone advised please.Maybe youre using the same tissue tek cryo 3... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Billing IHC on MOHS
I'd be very careful with this. Histochemical even if it's on frozen section such as 88314 does not mean immunohistochemical which is 88342 to my understanding. To my understanding anytime you use a antibody it's a 88342 code. Now as far as being able to code for each margin say from 3-6 or 6-9 o'clock etc that seems like it would fall under the rules for each site to me kind of like we would charge a 88307 or 88309 for excision with margins instead of a 88305 which pays less. I personally recommend this not be left in the hands of google or even histonet. Good information comes from both but you might just want to put a call into American society of MOHs and ask. Because over billing is serious business and can get you in a lot of trouble. I only say this because usually in MOHs you'll get the whole specimen which your checking for margins. Then any piece after that would be a separate specimen example that would be part " B " which you could charge separate for. And since different docs do this differently making a call couldn't hurt then you could come and share what they say with us :) Sent from my iPhone On Jun 15, 2012, at 2:20 PM, "Carol Torrence" wrote: > By George I've got it! This is what I read from "Coder's Choice CPT Plus" > > 88314 is for IHC performed on frozen tissue...when a nonroutine > histochemical stain on frozen tissue during Mohs surgery is utilized, report > 88314 with modifier 59. Report one unit of 88314 for each special stain on > each frozen surgical pathology block. > > Carol M. Torrence, HT(ASCP)CM > Director of Laboratory Services > KMC Dermatology - Pathology > 2921 SW Wanamaker Dr. > Topeka, Kansas 66614-5334 > 785-273-2788 ext 328 > fax 785-272-6185 > ctorre...@kmcpa.com > > > > > > > > > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
