[Histonet] RNA isolation form stained slides
Hello, I need to extract RNA for a RT-PCR after Laser Micro Dissection on xGal stained slides. I tried using sections from unfixed frozen organs. I fixed the sections in EtOH70% for 10min and then I stained them with xGal for 3h at 37°C. After air drying I cut out with the LCM and extract RNA with the PicoPure kit from Applied Biosystem. So far I didn’t manage to get enough RNA. I tried to add RNase inhibitors to all the solutions but it didn’t help. Any idea/suggestion? Do someone think it would be better to do a LacZ antibody staining on FFPE sections and extract RNA with an appropriate kit? The RNase would they be less active? Thank in advance for any help you can give me J Vanessa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Devasting news on 88305TC component
It was cut 52%. I cannot believe this. http://www.cap.org/apps/cap.portal?_nfpb=truecntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow_windowLabel=cntvwrPtltcntvwrPtlt%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_2013_physician_fee_schedule.html_state=maximized_pageLabel=cntvwr CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Devasting news on 88305TC component
Devastating I meant CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Softening toenails procedure
Hi, I need a good procedure for softening toe nails to run on HE. I have used Nair and KOH. Anyone have a good procedure that works well that they would like to share. Thanks, Nicole Tatum HT, ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Free Diagnostic Pathology Software from the UK - Cancer reporting proformas added
Some of the list members might be interested in some recent enhancements to the free diagnostic pathology software that we have developed in the UK. We are adding proformas for complex cancer cases based on the The International Collaboration on Cancer Reporting (ICCR) proformas that are being developed. You can trial this free software (version 2.1) and download it from this website: www.freedp.org Many histopathology laboratories in the UK are using antiquated reporting software. Upgrading to a more modern system is expensive. Even the more modern systems often lack the technology needed for pathologists to efficiently report complex cases. They are often difficult to use across multiple sites. We have developed reporting software that overcomes some of these difficulties. Over the last year we have shared this open source software internationally via the website above and there are now hundreds of users around the World. Over the next year we are planning to incorporate more cancer proformas, and will release an updated version of the software in early 2013, via the website above. I would be very for any feed back regarding bugs or ideas for enhancements. Fred Mayall Consultant Histopathologist Taunton and Somerset NHS Trust ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Softening toenails procedure
Under separate cover I am sending something I wrote about this topic. René J. From: Nicole Tatum nic...@dlcjax.com To: histonet@lists.utsouthwestern.edu Sent: Friday, November 2, 2012 8:05 AM Subject: [Histonet] Softening toenails procedure Hi, I need a good procedure for softening toe nails to run on HE. I have used Nair and KOH. Anyone have a good procedure that works well that they would like to share. Thanks, Nicole Tatum HT, ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Devasting news on 88305TC component
That is devastating! Do you have a link to this information? Sent from my iPhone On Nov 2, 2012, at 4:53 AM, Webster, Thomas S. twebs...@crh.org wrote: Devastating I meant CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Devasting news on 88305TC component
http://www.cap.org/apps/cap.portal?_nfpb=truecntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow_windowLabel=cntvwrPtltcntvwrPtlt%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_2013_physician_fee_schedule.html_state=maximized_pageLabel=cntvwr Brendal Finlay, HT (ASCP) Medical Center Clinic brendal.fin...@medicalcenterclinic.com 850.474.8758 http://medicalcenterclinic.com -Original message- From: Davide Costanzo pathloc...@gmail.com Date: Fri, 02 Nov 2012 10:09:18 -0500 To: Webster, Thomas S. twebs...@crh.org Subject: Re: [Histonet] Devasting news on 88305TCcomponent That is devastating! Do you have a link to this information? Sent from my iPhone On Nov 2, 2012, at 4:53 AM, Webster, Thomas S. wrote: Devastating I meant CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RNA isolation form stained slides
Have you tried using more sections during extraction? Can you extract into a smaller volume? On Friday, November 2, 2012, Vanessa Orsini wrote: Hello, I need to extract RNA for a RT-PCR after Laser Micro Dissection on xGal stained slides. I tried using sections from unfixed frozen organs. I fixed the sections in EtOH70% for 10min and then I stained them with xGal for 3h at 37°C. After air drying I cut out with the LCM and extract RNA with the PicoPure kit from Applied Biosystem. So far I didn’t manage to get enough RNA. I tried to add RNase inhibitors to all the solutions but it didn’t help. Any idea/suggestion? Do someone think it would be better to do a LacZ antibody staining on FFPE sections and extract RNA with an appropriate kit? The RNase would they be less active? Thank in advance for any help you can give me J Vanessa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu javascript:; http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Control tissue
Hi, Can anyone tell me if the control blocks we use for special stains and IHC need to have the accession number of the original case they came from on them? Does this info have to be put on the slides? Are there any CAP regulations about this? Thank you so much for your help. Deloris Carter HT(ASCP) Shawnee Mission Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ALK Positive lung slides
Does anybody have any lung tissue slides that are known to be positive for the ALK gene rearrangement or know of a source to obtain them? We are trying to validate this FISH assay are having a very difficult time finding any positive specimens (we have Abbott's positive control slides, but they do not look like real tissue specimens)Thanks!! Jill A. Johns, MT(ASCP)SH, QCym, CCy Manager of Flow Cytometry and Molecular Diagnostics Central PA Alliance Laboratory (CPAL) 1803 Mt. Rose Ave., Suite C3/C4 York, PA17403 phone: (717) 851-4320 fax: (717) 851-1450 email: jjoh...@cpallab.commailto:jjoh...@cpallab.com __ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Devasting news on 88305TC component
AMEN -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio Sent: Friday, November 02, 2012 9:32 AM To: Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: Re: [Histonet] Devasting news on 88305TC component Political yet?! Seriously! 52%, while the PC is increased 2%... But in case anyone wondered both candidates for President are looking for the middle class! Unbelievable! Sent from my iPhone On Nov 2, 2012, at 8:28 AM, Brendal Finlay brendal.fin...@medicalcenterclinic.com wrote: http://www.cap.org/apps/cap.portal?_nfpb=truecntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow_windowLabel=cntvwrPtltcntvwrPtlt%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_2013_physician_fee_schedule.html_state=maximized_pageLabel=cntvwr Brendal Finlay, HT (ASCP) Medical Center Clinic brendal.fin...@medicalcenterclinic.com 850.474.8758 http://medicalcenterclinic.com -Original message- From: Davide Costanzo pathloc...@gmail.com Date: Fri, 02 Nov 2012 10:09:18 -0500 To: Webster, Thomas S. twebs...@crh.org Subject: Re: [Histonet] Devasting news on 88305TCcomponent That is devastating! Do you have a link to this information? Sent from my iPhone On Nov 2, 2012, at 4:53 AM, Webster, Thomas S. wrote: Devastating I meant CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Devasting news on 88305TC component
Before you holler political and blame the candidates, ask yourself who was hurt most by POL's? Large reference labs. With this change they will get back the business because it will not be profitable to establish a POL. Also they lobbied for and increase on the PC, reference have pathologist, most POL's don't. Just my thought -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Friday, November 02, 2012 11:52 AM To: 'Cristi Rigazio'; Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: RE: [Histonet] Devasting news on 88305TC component AMEN -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio Sent: Friday, November 02, 2012 9:32 AM To: Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: Re: [Histonet] Devasting news on 88305TC component Political yet?! Seriously! 52%, while the PC is increased 2%... But in case anyone wondered both candidates for President are looking for the middle class! Unbelievable! Sent from my iPhone On Nov 2, 2012, at 8:28 AM, Brendal Finlay brendal.fin...@medicalcenterclinic.com wrote: http://www.cap.org/apps/cap.portal?_nfpb=truecntvwrPtlt_actionOverrid e=%2Fportlets%2FcontentViewer%2Fshow_windowLabel=cntvwrPtltcntvwrPtl t%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_201 3_physician_fee_schedule.html_state=maximized_pageLabel=cntvwr Brendal Finlay, HT (ASCP) Medical Center Clinic brendal.fin...@medicalcenterclinic.com 850.474.8758 http://medicalcenterclinic.com -Original message- From: Davide Costanzo pathloc...@gmail.com Date: Fri, 02 Nov 2012 10:09:18 -0500 To: Webster, Thomas S. twebs...@crh.org Subject: Re: [Histonet] Devasting news on 88305TCcomponent That is devastating! Do you have a link to this information? Sent from my iPhone On Nov 2, 2012, at 4:53 AM, Webster, Thomas S. wrote: Devastating I meant CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Devasting news on 88305TC component
Actually, the global payment for this code is being reduced by 33% according to the announcement. Regardless of the establishment, small lab; large volume lab; POL lab everyone will be taking a hit on this. Even if the POL labs all dried up and went away, the remaining labs may get the work but they will be doing it for a lot less reimbursement. Kari Kienitz HT, (ASCP) Histology Laboratory Portland Gastroenterology The Oregon Clinic NE 99th Ave Portland, OR 97220 503.935.8311 kkien...@orclinic.com From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton [flna...@texaschildrens.org] Sent: Friday, November 02, 2012 10:01 AM To: 'Jesus Ellin'; 'Cristi Rigazio'; Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: RE: [Histonet] Devasting news on 88305TC component Before you holler political and blame the candidates, ask yourself who was hurt most by POL's? Large reference labs. With this change they will get back the business because it will not be profitable to establish a POL. Also they lobbied for and increase on the PC, reference have pathologist, most POL's don't. Just my thought -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Friday, November 02, 2012 11:52 AM To: 'Cristi Rigazio'; Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: RE: [Histonet] Devasting news on 88305TC component AMEN -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio Sent: Friday, November 02, 2012 9:32 AM To: Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: Re: [Histonet] Devasting news on 88305TC component Political yet?! Seriously! 52%, while the PC is increased 2%... But in case anyone wondered both candidates for President are looking for the middle class! Unbelievable! Sent from my iPhone On Nov 2, 2012, at 8:28 AM, Brendal Finlay brendal.fin...@medicalcenterclinic.com wrote: http://www.cap.org/apps/cap.portal?_nfpb=truecntvwrPtlt_actionOverrid e=%2Fportlets%2FcontentViewer%2Fshow_windowLabel=cntvwrPtltcntvwrPtl t%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_201 3_physician_fee_schedule.html_state=maximized_pageLabel=cntvwr Brendal Finlay, HT (ASCP) Medical Center Clinic brendal.fin...@medicalcenterclinic.com 850.474.8758 http://medicalcenterclinic.com -Original message- From: Davide Costanzo pathloc...@gmail.com Date: Fri, 02 Nov 2012 10:09:18 -0500 To: Webster, Thomas S. twebs...@crh.org Subject: Re: [Histonet] Devasting news on 88305TCcomponent That is devastating! Do you have a link to this information? Sent from my iPhone On Nov 2, 2012, at 4:53 AM, Webster, Thomas S. wrote: Devastating I meant CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. __ ___ Histonet mailing list
Re: [Histonet] Devasting news on 88305TC component
EXACTLY SO! POLs started to offer better prices to interested colleagues and by doing so work started to drain into POLs with the pointed out result = less work for Reference labs. Do you think that large ref. labs like Quest or Lab.Corp were going to take that drainage sitting on their hands? Sure not! They are business with billions at stake and money to lobby. This is the result. Blame the power of the lobbyists! Policies and even sometimes democracy is tainted by big money. Tell that to the Supreme Court that has ruled that a large company is a person. René J. From: Nails, Felton flna...@texaschildrens.org To: 'Jesus Ellin' jel...@yumaregional.org; 'Cristi Rigazio' cls71...@gmail.com; Brendal Finlay brendal.fin...@medicalcenterclinic.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu; Webster, Thomas S. twebs...@crh.org Sent: Friday, November 2, 2012 1:01 PM Subject: RE: [Histonet] Devasting news on 88305TC component Before you holler political and blame the candidates, ask yourself who was hurt most by POL's? Large reference labs. With this change they will get back the business because it will not be profitable to establish a POL. Also they lobbied for and increase on the PC, reference have pathologist, most POL's don't. Just my thought -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Friday, November 02, 2012 11:52 AM To: 'Cristi Rigazio'; Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: RE: [Histonet] Devasting news on 88305TC component AMEN -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio Sent: Friday, November 02, 2012 9:32 AM To: Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: Re: [Histonet] Devasting news on 88305TC component Political yet?! Seriously! 52%, while the PC is increased 2%... But in case anyone wondered both candidates for President are looking for the middle class! Unbelievable! Sent from my iPhone On Nov 2, 2012, at 8:28 AM, Brendal Finlay brendal.fin...@medicalcenterclinic.com wrote: http://www.cap.org/apps/cap.portal?_nfpb=truecntvwrPtlt_actionOverrid e=%2Fportlets%2FcontentViewer%2Fshow_windowLabel=cntvwrPtltcntvwrPtl t%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_201 3_physician_fee_schedule.html_state=maximized_pageLabel=cntvwr Brendal Finlay, HT (ASCP) Medical Center Clinic brendal.fin...@medicalcenterclinic.com 850.474.8758 http://medicalcenterclinic.com -Original message- From: Davide Costanzo pathloc...@gmail.com Date: Fri, 02 Nov 2012 10:09:18 -0500 To: Webster, Thomas S. twebs...@crh.org Subject: Re: [Histonet] Devasting news on 88305TCcomponent That is devastating! Do you have a link to this information? Sent from my iPhone On Nov 2, 2012, at 4:53 AM, Webster, Thomas S. wrote: Devastating I meant CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You.
RE: [Histonet] Control tissue
We put the accession number of the control tissue used on the slide and the information is also entered into our database. This eliminates the need for keeping an additional separate record for control tissue identification. Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Friday, November 02, 2012 10:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Control tissue Hi, Can anyone tell me if the control blocks we use for special stains and IHC need to have the accession number of the original case they came from on them? Does this info have to be put on the slides? Are there any CAP regulations about this? Thank you so much for your help. Deloris Carter HT(ASCP) Shawnee Mission Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] ALK Positive lung slides
Hi Jill, Have you tried using cases that you've already sent out to other labs and they've reported as ALK-positive? That is how we validated this assay in my lab. Most cases aren't very positive (you only have to find 15 cells out of a hundred that you count to call the case positive if a second observer agrees). You're supposed to look around for positive cells using your dual green/red filter. Since most tumor cells show a fused yellow or adjacent red green signals, you really do have to look around for them a little. Because of that, I'd suggest doing the validation with cases that have a range of % of positve cells. If another lab calls a case positive at 25% positive cells then you'd want to get that result too most of the time. That being said, I can send you some de-identified slides from a case of metastatic (to a node) NSCLC that is super positive. I do think it is helpful to see what a case with a very high % of positive cells look like. This is what we're using as our positive control. We think it's better to have a tissue control than the cell cultured cells, processed into cell blocks, that are on the slides sold by Abbott. Send me your address and fedex # if interested. Mark On Fri, Nov 2, 2012 at 9:20 AM, Johns, Jill jjoh...@cpallab.com wrote: Does anybody have any lung tissue slides that are known to be positive for the ALK gene rearrangement or know of a source to obtain them? We are trying to validate this FISH assay are having a very difficult time finding any positive specimens (we have Abbott's positive control slides, but they do not look like real tissue specimens)Thanks!! Jill A. Johns, MT(ASCP)SH, QCym, CCy Manager of Flow Cytometry and Molecular Diagnostics Central PA Alliance Laboratory (CPAL) 1803 Mt. Rose Ave., Suite C3/C4 York, PA17403 phone: (717) 851-4320 fax: (717) 851-1450 email: jjoh...@cpallab.commailto:jjoh...@cpallab.com __ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Control tissue
I would think that ANP.21100, 21366 might be applied by an inspector ( from older checklist so sorry numbers might have changed). But in any case I might suggest that some sort of numbering system to indicate the block sequence/identity and tissue type of the control is needed. Though you could probably write that accession number, it might use up too much writing space depending on what else you labeling requires. I think the checklist from last year had a specific stipulation about this. Probably on the CAP site - cap.org- by now. As I interpret it, you need a tracking of the source material and record keeping of that and what block was used as a control on what patient sample. You should also document that it worked :) Using a log to record the original source has usually worked out, and you can document it once as the original source with a tracking number to any prepared control blocks or control slides. I have always done testing of controls ( first and last of large batches) , or at least parallel, run noting the source, before use with a patient sample, and document the results are as expected- but I don't always see labs doing this. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Fri, 2 Nov 2012 11:09:01 -0500 From: dels...@gmail.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Control tissue Hi, Can anyone tell me if the control blocks we use for special stains and IHC need to have the accession number of the original case they came from on them? Does this info have to be put on the slides? Are there any CAP regulations about this? Thank you so much for your help. Deloris Carter HT(ASCP) Shawnee Mission Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RNA isolation form stained slides
I would try getting more sections from LCM and extracting into 7.5 uL, if
that volume will be enough to for your PCR reactions.
On Friday, November 2, 2012, Vanessa Orsini wrote:
With the kit I'm extracting in 10ulthe problem is that I have too use
few stained cells isolated with the LCM...so even if I increase the number
of slides I'll never have a lot of material...
Inviato da iPhone
Il giorno 2 nov. 2012, alle ore 16:44, Mark Tarango
marktara...@gmail.com javascript:_e({}, 'cvml',
'marktara...@gmail.com'); ha scritto:
Have you tried using more sections during extraction? Can you extract
into a smaller volume?
On Friday, November 2, 2012, Vanessa Orsini wrote:
Hello,
I need to extract RNA for a RT-PCR after Laser Micro
Dissection on xGal stained slides.
I tried using sections from unfixed frozen organs. I fixed the
sections in EtOH70% for 10min and then I stained them with xGal for 3h at
37°C.
After air drying I cut out with the LCM and extract RNA with the PicoPure
kit
from Applied Biosystem. So far I didn’t manage to get enough RNA.
I tried to add RNase inhibitors to all the solutions but it
didn’t help.
Any idea/suggestion?
Do someone think it would be better to do a LacZ antibody staining
on FFPE sections and extract RNA with an appropriate kit? The RNase would
they
be less active?
Thank in advance for any help you can give me J Vanessa
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Re: [Histonet] Devasting news on 88305TC component
The Gravy Train has left the station. Jay A. Lundgren, M.S., HTL (ASCP) On Fri, Nov 2, 2012 at 12:26 PM, Rene J Buesa rjbu...@yahoo.com wrote: EXACTLY SO! POLs started to offer better prices to interested colleagues and by doing so work started to drain into POLs with the pointed out result = less work for Reference labs. Do you think that large ref. labs like Quest or Lab.Corp were going to take that drainage sitting on their hands? Sure not! They are business with billions at stake and money to lobby. This is the result. Blame the power of the lobbyists! Policies and even sometimes democracy is tainted by big money. Tell that to the Supreme Court that has ruled that a large company is a person. René J. From: Nails, Felton flna...@texaschildrens.org To: 'Jesus Ellin' jel...@yumaregional.org; 'Cristi Rigazio' cls71...@gmail.com; Brendal Finlay brendal.fin...@medicalcenterclinic.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu; Webster, Thomas S. twebs...@crh.org Sent: Friday, November 2, 2012 1:01 PM Subject: RE: [Histonet] Devasting news on 88305TC component Before you holler political and blame the candidates, ask yourself who was hurt most by POL's? Large reference labs. With this change they will get back the business because it will not be profitable to establish a POL. Also they lobbied for and increase on the PC, reference have pathologist, most POL's don't. Just my thought -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Friday, November 02, 2012 11:52 AM To: 'Cristi Rigazio'; Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: RE: [Histonet] Devasting news on 88305TC component AMEN -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio Sent: Friday, November 02, 2012 9:32 AM To: Brendal Finlay Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S. Subject: Re: [Histonet] Devasting news on 88305TC component Political yet?! Seriously! 52%, while the PC is increased 2%... But in case anyone wondered both candidates for President are looking for the middle class! Unbelievable! Sent from my iPhone On Nov 2, 2012, at 8:28 AM, Brendal Finlay brendal.fin...@medicalcenterclinic.com wrote: http://www.cap.org/apps/cap.portal?_nfpb=truecntvwrPtlt_actionOverrid e=%2Fportlets%2FcontentViewer%2Fshow_windowLabel=cntvwrPtltcntvwrPtl t%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_201 3_physician_fee_schedule.html_state=maximized_pageLabel=cntvwr Brendal Finlay, HT (ASCP) Medical Center Clinic brendal.fin...@medicalcenterclinic.com 850.474.8758 http://medicalcenterclinic.com -Original message- From: Davide Costanzo pathloc...@gmail.com Date: Fri, 02 Nov 2012 10:09:18 -0500 To: Webster, Thomas S. twebs...@crh.org Subject: Re: [Histonet] Devasting news on 88305TCcomponent That is devastating! Do you have a link to this information? Sent from my iPhone On Nov 2, 2012, at 4:53 AM, Webster, Thomas S. wrote: Devastating I meant CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you
RE: [Histonet] RNA isolation form stained slides
Have you tried to extract the RNA from the tissue without the stain- just to test your method? I am not familiar with the mechanics of the stain but you might be denaturing the RNA. Also are you sure there is enough message in the area you are trying to extract to get enough RNA? Maybe you need more material. Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Orsini Sent: Friday, November 02, 2012 5:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RNA isolation form stained slides Hello, I need to extract RNA for a RT-PCR after Laser MicroDissection on xGal stained slides. I tried using sections from unfixed frozen organs. I fixed the sections in EtOH70% for 10min and then I stained them with xGal for 3h at 37°C. After air drying I cut out with the LCM and extract RNA with the PicoPure kit from Applied Biosystem. So far I didn't manage to get enough RNA. I tried to add RNase inhibitors to all the solutions but it didn't help. Any idea/suggestion? Do someone think it would be better to do a LacZ antibody staining on FFPE sections and extract RNA with an appropriate kit? The RNase would they be less active? Thank in advance for any help you can give me J Vanessa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
