RE: [Histonet] RNA isolation form stained slides

2012-11-02 Thread Sue Hunter
Have you tried to extract the RNA from the tissue without the stain- just to 
test your method?  I am not familiar with the mechanics of the stain but you 
might be denaturing the RNA.  Also are you sure there is enough message in the 
area you are trying to extract to get enough RNA?  Maybe you need more material.

Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shun...@beaumont.edu


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Orsini
Sent: Friday, November 02, 2012 5:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RNA isolation form stained slides







Hello,

I need to extract RNA for a RT-PCR after Laser MicroDissection on xGal stained 
slides.

I tried using sections from unfixed frozen organs. I fixed the sections in 
EtOH70% for 10min and then I stained them with xGal for 3h at 37°C.
After air drying I cut out with the LCM and extract RNA with the PicoPure kit 
from Applied Biosystem. So far I didn't manage to get enough RNA. 

I tried to add RNase inhibitors to all the solutions but it didn't help.

 

Any idea/suggestion?

Do someone think it would be better to do a LacZ antibody staining on FFPE 
sections and extract RNA with an appropriate kit? The RNase would they be less 
active?

 

Thank in advance for any help you can give me J Vanessa

  
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Re: [Histonet] Devasting news on 88305TC component

2012-11-02 Thread Jay Lundgren
The Gravy Train has left the station.

 Jay A. Lundgren, M.S., HTL
(ASCP)


On Fri, Nov 2, 2012 at 12:26 PM, Rene J Buesa  wrote:

> EXACTLY SO!
> POLs started to offer "better prices" to interested colleagues and by
> doing so work started to "drain" into POLs with the pointed out result =
> less work for Reference labs.
> Do you think that large ref. labs like Quest or Lab.Corp were going to
> "take that drainage" sitting on their hands?
> Sure not! They are business with billions at stake and money to lobby.
> This is the result. Blame the power of the lobbyists!
> Policies and even sometimes democracy is tainted by big money.
> Tell that to the Supreme Court that has ruled that a large company is
> a "person".
> René J.
>
>
> 
> From: "Nails, Felton" 
> To: 'Jesus Ellin' ; 'Cristi Rigazio' <
> cls71...@gmail.com>; Brendal Finlay <
> brendal.fin...@medicalcenterclinic.com>
> Cc: "histonet@lists.utsouthwestern.edu" ;
> "Webster, Thomas S." 
> Sent: Friday, November 2, 2012 1:01 PM
> Subject: RE: [Histonet] Devasting news on 88305TC component
>
> Before you holler political and blame the candidates, ask yourself who was
> hurt most by POL's?
> Large reference labs.
> With this change they will get back the business because it will not be
> profitable to establish a POL.
> Also they lobbied for and increase on the PC, reference have pathologist,
> most POL's don't.
> Just my thought
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu [mailto:
> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin
> Sent: Friday, November 02, 2012 11:52 AM
> To: 'Cristi Rigazio'; Brendal Finlay
> Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S.
> Subject: RE: [Histonet] Devasting news on 88305TC component
>
> AMEN
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu [mailto:
> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio
> Sent: Friday, November 02, 2012 9:32 AM
> To: Brendal Finlay
> Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S.
> Subject: Re: [Histonet] Devasting news on 88305TC component
>
> Political yet?!  Seriously!  52%, while the PC is increased 2%... But in
> case anyone wondered both candidates for President are looking for the
> middle class!  Unbelievable!
>
> Sent from my iPhone
>
> On Nov 2, 2012, at 8:28 AM, "Brendal Finlay" <
> brendal.fin...@medicalcenterclinic.com> wrote:
>
> > http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid
> > e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl
> > t%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_201
> > 3_physician_fee_schedule.html&_state=maximized&_pageLabel=cntvwr
> >
> >
> > Brendal Finlay, HT (ASCP)
> > Medical Center Clinic
> > brendal.fin...@medicalcenterclinic.com
> > 850.474.8758
> > http://medicalcenterclinic.com
> > -Original message-
> > From: Davide Costanzo pathloc...@gmail.com
> > Date: Fri, 02 Nov 2012 10:09:18 -0500
> > To: "Webster, Thomas S." twebs...@crh.org
> > Subject: Re: [Histonet] Devasting news on 88305TCcomponent
> >
> > That is devastating! Do you have a link to this information?
> >
> > Sent from my iPhone
> >
> > On Nov 2, 2012, at 4:53 AM, "Webster, Thomas S." wrote:
> >
> >> Devastating I meant
> >>
> >>
> >> CONFIDENTIALITY NOTICE:
> >> This e-mail message, including all attachments, is for the sole use
> > of the
> >> intended recipient(s) and may contain confidential and privileged
> >> information. You may NOT use, disclose, copy or disseminate this
> >> information. If you are not the intended recipient, please contact
> > the
> >> sender by reply e-mail immediately. Please destroy all copies of the
> >> original message and all attachments. Your cooperation is greatly
> >> appreciated.
> >> Columbus Regional Hospital
> >> 2400 East 17th Street
> >> Columbus, Indiana
> > 47201___
> >> Histonet mailing list
> >> Histonet@lists.utsouthwestern.edu
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> disclosure under applicable law.  If you are not the intended recipient(s),
> you are no

Re: [Histonet] RNA isolation form stained slides

2012-11-02 Thread Mark Tarango
 I would try getting more sections from LCM and extracting into 7.5 uL, if
that volume will be enough to for your PCR reactions.

On Friday, November 2, 2012, Vanessa Orsini wrote:

> With the kit I'm extracting in 10ulthe problem is that I have too use
> few stained cells isolated with the LCM...so even if I increase the number
> of slides I'll never have a lot of material...
>
> Inviato da iPhone
>
> Il giorno 2 nov. 2012, alle ore 16:44, "Mark Tarango" <
> marktara...@gmail.com  'marktara...@gmail.com');>> ha scritto:
>
> Have you tried using more sections during extraction?  Can you extract
> into a smaller volume?
>
> On Friday, November 2, 2012, Vanessa Orsini wrote:
>
>>
>>
>>
>>
>>
>>
>> Hello,
>>
>> I need to extract RNA for a RT-PCR after Laser Micro
>> Dissection on xGal stained slides.
>>
>> I tried using sections from unfixed frozen organs. I fixed the
>> sections in EtOH70% for 10min and then I stained them with xGal for 3h at
>> 37°C.
>> After air drying I cut out with the LCM and extract RNA with the PicoPure
>> kit
>> from Applied Biosystem. So far I didn’t manage to get enough RNA.
>>
>> I tried to add RNase inhibitors to all the solutions but it
>> didn’t help.
>>
>>
>>
>> Any idea/suggestion?
>>
>> Do someone think it would be better to do a LacZ antibody staining
>> on FFPE sections and extract RNA with an appropriate kit? The RNase would
>> they
>> be less active?
>>
>>
>>
>> Thank in advance for any help you can give me J Vanessa
>>
>>
>> ___
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
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RE: [Histonet] Control tissue

2012-11-02 Thread joelle weaver

I would think that ANP.21100, 21366 might be applied by an inspector ( from 
older checklist so sorry numbers might have changed). But in any case I might 
suggest that some sort of numbering system to indicate the block 
sequence/identity and tissue type of the control is needed. Though you could 
probably write that accession number,  it might use up too much writing space 
depending on what else you labeling requires.  I think the checklist from last 
year had a specific stipulation about this. Probably on the CAP site - cap.org- 
by now.  As I interpret it, you need a tracking of the source material and 
record keeping of that and what block was used as a control on what patient 
sample. You should also document that it worked :) Using a log to record the 
original source has usually worked out, and you can document it once as the 
original source with a tracking number to any prepared control blocks or 
control slides. I have always done testing of controls ( first and last of 
large batches) , or at least  parallel,  run noting the source, before use with 
a patient sample, and document the results are as expected- but I don't always 
see labs doing this. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 > Date: Fri, 2 Nov 2012 11:09:01 -0500
> From: dels...@gmail.com
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Control tissue
> 
> Hi,
> Can anyone tell me if the control blocks we use for special stains and IHC
> need to have the accession number of the original case they came from on
> them?  Does this info have to be put on the slides?  Are there any CAP
> regulations about this?  Thank you so much for your help.
> Deloris Carter HT(ASCP)
> Shawnee Mission Medical Center
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
  
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Re: [Histonet] ALK Positive lung slides

2012-11-02 Thread Mark Tarango
Hi Jill,

Have you tried using cases that you've already sent out to other labs and
they've reported as ALK-positive?  That is how we validated this assay in
my lab.  Most cases aren't "very" positive (you only have to find 15 cells
out of a hundred that you count to call the case positive if a second
observer agrees).  You're supposed to look around for positive cells using
your dual green/red filter.  Since most tumor cells show a fused yellow or
adjacent red & green signals, you really do have to look around for them a
little.  Because of that, I'd suggest doing the validation with cases that
have a range of % of positve cells.  If another lab calls a case positive
at 25% positive cells then you'd want to get that result too most of the
time.

That being said, I can send you some de-identified slides from a case of
metastatic (to a node) NSCLC that is "super" positive.  I do think it is
helpful to see what a case with a very high % of positive cells look
like.  This is what we're using as our positive control.  We think it's
better to have a tissue control than the cell cultured cells, processed
into cell blocks, that are on the slides sold by Abbott.  Send me your
address and fedex # if interested.

Mark


On Fri, Nov 2, 2012 at 9:20 AM, Johns, Jill  wrote:

> Does anybody have any lung tissue slides that are known to be positive for
> the ALK gene rearrangement or know of a source to obtain them? We are
> trying to validate this FISH assay are having a very difficult time finding
> any positive specimens (we have Abbott's positive control slides, but they
> do not look like "real" tissue specimens)Thanks!!
>
> Jill A. Johns, MT(ASCP)SH, QCym, CCy
> Manager of Flow Cytometry and Molecular Diagnostics
> Central PA Alliance Laboratory (CPAL)
> 1803 Mt. Rose Ave., Suite C3/C4
> York,  PA17403
> phone: (717) 851-4320
> fax:  (717) 851-1450
> email:  jjoh...@cpallab.com
>
>
> __
> This e-mail has been scanned by Verizon Managed Email Content Service,
> using Skeptic(tm) technology powered by MessageLabs. For more information
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RE: [Histonet] Control tissue

2012-11-02 Thread Goins, Tresa
We put the accession number of the control tissue used on the slide and the 
information is also entered into our database.  This eliminates the need for 
keeping an additional separate record for control tissue identification.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Deloris Carter
Sent: Friday, November 02, 2012 10:09 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Control tissue

Hi,
Can anyone tell me if the control blocks we use for special stains and IHC need 
to have the accession number of the original case they came from on them?  Does 
this info have to be put on the slides?  Are there any CAP regulations about 
this?  Thank you so much for your help.
Deloris Carter HT(ASCP)
Shawnee Mission Medical Center
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Re: [Histonet] Devasting news on 88305TC component

2012-11-02 Thread Rene J Buesa
EXACTLY SO!
POLs started to offer "better prices" to interested colleagues and by doing so 
work started to "drain" into POLs with the pointed out result = less work for 
Reference labs.
Do you think that large ref. labs like Quest or Lab.Corp were going to "take 
that drainage" sitting on their hands?
Sure not! They are business with billions at stake and money to lobby.
This is the result. Blame the power of the lobbyists!
Policies and even sometimes democracy is tainted by big money.
Tell that to the Supreme Court that has ruled that a large company is 
a "person". 
René J.



From: "Nails, Felton" 
To: 'Jesus Ellin' ; 'Cristi Rigazio' 
; Brendal Finlay  
Cc: "histonet@lists.utsouthwestern.edu" ; 
"Webster, Thomas S."  
Sent: Friday, November 2, 2012 1:01 PM
Subject: RE: [Histonet] Devasting news on 88305TC component

Before you holler political and blame the candidates, ask yourself who was hurt 
most by POL's?
Large reference labs.
With this change they will get back the business because it will not be 
profitable to establish a POL.
Also they lobbied for and increase on the PC, reference have pathologist, most 
POL's don't.
Just my thought 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin
Sent: Friday, November 02, 2012 11:52 AM
To: 'Cristi Rigazio'; Brendal Finlay
Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S.
Subject: RE: [Histonet] Devasting news on 88305TC component

AMEN

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio
Sent: Friday, November 02, 2012 9:32 AM
To: Brendal Finlay
Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S.
Subject: Re: [Histonet] Devasting news on 88305TC component

Political yet?!  Seriously!  52%, while the PC is increased 2%... But in case 
anyone wondered both candidates for President are looking for the middle 
class!  Unbelievable!

Sent from my iPhone

On Nov 2, 2012, at 8:28 AM, "Brendal Finlay" 
 wrote:

> http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid
> e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl
> t%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_201
> 3_physician_fee_schedule.html&_state=maximized&_pageLabel=cntvwr
> 
> 
> Brendal Finlay, HT (ASCP)
> Medical Center Clinic
> brendal.fin...@medicalcenterclinic.com
> 850.474.8758
> http://medicalcenterclinic.com
> -Original message-
> From: Davide Costanzo pathloc...@gmail.com
> Date: Fri, 02 Nov 2012 10:09:18 -0500
> To: "Webster, Thomas S." twebs...@crh.org
> Subject: Re: [Histonet] Devasting news on 88305TCcomponent
> 
> That is devastating! Do you have a link to this information?
> 
> Sent from my iPhone
> 
> On Nov 2, 2012, at 4:53 AM, "Webster, Thomas S." wrote:
> 
>> Devastating I meant
>> 
>> 
>> CONFIDENTIALITY NOTICE:
>> This e-mail message, including all attachments, is for the sole use
> of the
>> intended recipient(s) and may contain confidential and privileged 
>> information. You may NOT use, disclose, copy or disseminate this 
>> information. If you are not the intended recipient, please contact
> the
>> sender by reply e-mail immediately. Please destroy all copies of the 
>> original message and all attachments. Your cooperation is greatly 
>> appreciated.
>> Columbus Regional Hospital
>> 2400 East 17th Street
>> Columbus, Indiana
> 47201___
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> ___
> Histonet mailing list
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This message is confidential, intended only for the named
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are notified that the dissemination, distribution, or copying of this message 
is strictly prohibited.  If you receive this message in error, or are not the 
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RE: [Histonet] Devasting news on 88305TC component

2012-11-02 Thread Kienitz, Kari
Actually, the global payment for this code is being reduced by 33% according to 
the announcement. Regardless of the establishment, small lab; large volume lab; 
POL lab everyone will be taking a hit on this.  Even if the POL labs all dried 
up and went away, the remaining labs may get the work but they will be doing it 
for a lot less reimbursement. 


Kari Kienitz HT, (ASCP)
Histology Laboratory
Portland Gastroenterology
The Oregon Clinic
 NE 99th Ave
Portland, OR  97220
503.935.8311
kkien...@orclinic.com

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton 
[flna...@texaschildrens.org]
Sent: Friday, November 02, 2012 10:01 AM
To: 'Jesus Ellin'; 'Cristi Rigazio'; Brendal Finlay
Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S.
Subject: RE: [Histonet] Devasting news on 88305TC component

Before you holler political and blame the candidates, ask yourself who was hurt 
most by POL's?
Large reference labs.
With this change they will get back the business because it will not be 
profitable to establish a POL.
Also they lobbied for and increase on the PC, reference have pathologist, most 
POL's don't.
Just my thought

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin
Sent: Friday, November 02, 2012 11:52 AM
To: 'Cristi Rigazio'; Brendal Finlay
Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S.
Subject: RE: [Histonet] Devasting news on 88305TC component

AMEN

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio
Sent: Friday, November 02, 2012 9:32 AM
To: Brendal Finlay
Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S.
Subject: Re: [Histonet] Devasting news on 88305TC component

Political yet?!  Seriously!  52%, while the PC is increased 2%... But in case 
anyone wondered both candidates for President are looking for the middle class! 
 Unbelievable!

Sent from my iPhone

On Nov 2, 2012, at 8:28 AM, "Brendal Finlay" 
 wrote:

> http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid
> e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl
> t%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_201
> 3_physician_fee_schedule.html&_state=maximized&_pageLabel=cntvwr
>
>
> Brendal Finlay, HT (ASCP)
> Medical Center Clinic
> brendal.fin...@medicalcenterclinic.com
> 850.474.8758
> http://medicalcenterclinic.com
> -Original message-
> From: Davide Costanzo pathloc...@gmail.com
> Date: Fri, 02 Nov 2012 10:09:18 -0500
> To: "Webster, Thomas S." twebs...@crh.org
> Subject: Re: [Histonet] Devasting news on 88305TCcomponent
>
> That is devastating! Do you have a link to this information?
>
> Sent from my iPhone
>
> On Nov 2, 2012, at 4:53 AM, "Webster, Thomas S." wrote:
>
>> Devastating I meant
>>
>>
>> CONFIDENTIALITY NOTICE:
>> This e-mail message, including all attachments, is for the sole use
> of the
>> intended recipient(s) and may contain confidential and privileged
>> information. You may NOT use, disclose, copy or disseminate this
>> information. If you are not the intended recipient, please contact
> the
>> sender by reply e-mail immediately. Please destroy all copies of the
>> original message and all attachments. Your cooperation is greatly
>> appreciated.
>> Columbus Regional Hospital
>> 2400 East 17th Street
>> Columbus, Indiana
> 47201___
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ___
> Histonet mailing list
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This message is confidential, intended only for the named
recipient(s) and may contain information that is privileged or exempt from 
disclosure under applicable law.  If you are not the intended recipient(s), you 
are notified that the dissemination, distribution, or copying of this message 
is strictly prohibited.  If you receive this message in error, or are not the 
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Thank You.
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RE: [Histonet] Devasting news on 88305TC component

2012-11-02 Thread Nails, Felton
Before you holler political and blame the candidates, ask yourself who was hurt 
most by POL's?
Large reference labs.
With this change they will get back the business because it will not be 
profitable to establish a POL.
Also they lobbied for and increase on the PC, reference have pathologist, most 
POL's don't.
Just my thought 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin
Sent: Friday, November 02, 2012 11:52 AM
To: 'Cristi Rigazio'; Brendal Finlay
Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S.
Subject: RE: [Histonet] Devasting news on 88305TC component

AMEN

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio
Sent: Friday, November 02, 2012 9:32 AM
To: Brendal Finlay
Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S.
Subject: Re: [Histonet] Devasting news on 88305TC component

Political yet?!  Seriously!  52%, while the PC is increased 2%... But in case 
anyone wondered both candidates for President are looking for the middle class! 
 Unbelievable!

Sent from my iPhone

On Nov 2, 2012, at 8:28 AM, "Brendal Finlay" 
 wrote:

> http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverrid
> e=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtl
> t%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_201
> 3_physician_fee_schedule.html&_state=maximized&_pageLabel=cntvwr
> 
> 
> Brendal Finlay, HT (ASCP)
> Medical Center Clinic
> brendal.fin...@medicalcenterclinic.com
> 850.474.8758
> http://medicalcenterclinic.com
> -Original message-
> From: Davide Costanzo pathloc...@gmail.com
> Date: Fri, 02 Nov 2012 10:09:18 -0500
> To: "Webster, Thomas S." twebs...@crh.org
> Subject: Re: [Histonet] Devasting news on 88305TCcomponent
> 
> That is devastating! Do you have a link to this information?
> 
> Sent from my iPhone
> 
> On Nov 2, 2012, at 4:53 AM, "Webster, Thomas S." wrote:
> 
>> Devastating I meant
>> 
>> 
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RE: [Histonet] Devasting news on 88305TC component

2012-11-02 Thread Jesus Ellin
AMEN

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio
Sent: Friday, November 02, 2012 9:32 AM
To: Brendal Finlay
Cc: histonet@lists.utsouthwestern.edu; Webster, Thomas S.
Subject: Re: [Histonet] Devasting news on 88305TC component

Political yet?!  Seriously!  52%, while the PC is increased 2%... But in case 
anyone wondered both candidates for President are looking for the middle class! 
 Unbelievable!

Sent from my iPhone

On Nov 2, 2012, at 8:28 AM, "Brendal Finlay" 
 wrote:

> http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_2013_physician_fee_schedule.html&_state=maximized&_pageLabel=cntvwr
> 
> 
> Brendal Finlay, HT (ASCP)
> Medical Center Clinic
> brendal.fin...@medicalcenterclinic.com
> 850.474.8758
> http://medicalcenterclinic.com
> -Original message-
> From: Davide Costanzo pathloc...@gmail.com
> Date: Fri, 02 Nov 2012 10:09:18 -0500
> To: "Webster, Thomas S." twebs...@crh.org
> Subject: Re: [Histonet] Devasting news on 88305TCcomponent
> 
> That is devastating! Do you have a link to this information?
> 
> Sent from my iPhone
> 
> On Nov 2, 2012, at 4:53 AM, "Webster, Thomas S." wrote:
> 
>> Devastating I meant
>> 
>> 
>> CONFIDENTIALITY NOTICE:
>> This e-mail message, including all attachments, is for the sole use
> of the
>> intended recipient(s) and may contain confidential and privileged
>> information. You may NOT use, disclose, copy or disseminate this
>> information. If you are not the intended recipient, please contact
> the
>> sender by reply e-mail immediately. Please destroy all copies of the
>> original message and all attachments. Your cooperation is greatly
>> appreciated.
>> Columbus Regional Hospital
>> 2400 East 17th Street
>> Columbus, Indiana
> 47201___
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
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dissemination, distribution, or copying of this message is 
strictly prohibited.  If you receive this message in error, 
or are not the named recipient(s), please notify the sender 
at either the e-mail, fax, address, or telephone number 
listed above and delete this e-mail from your computer. 
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Re: [Histonet] Devasting news on 88305TC component

2012-11-02 Thread Cristi Rigazio
Political yet?!  Seriously!  52%, while the PC is increased 2%... But in case 
anyone wondered both candidates for President are looking for the middle class! 
 Unbelievable!

Sent from my iPhone

On Nov 2, 2012, at 8:28 AM, "Brendal Finlay" 
 wrote:

> http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_2013_physician_fee_schedule.html&_state=maximized&_pageLabel=cntvwr
> 
> 
> Brendal Finlay, HT (ASCP)
> Medical Center Clinic
> brendal.fin...@medicalcenterclinic.com
> 850.474.8758
> http://medicalcenterclinic.com
> -Original message-
> From: Davide Costanzo pathloc...@gmail.com
> Date: Fri, 02 Nov 2012 10:09:18 -0500
> To: "Webster, Thomas S." twebs...@crh.org
> Subject: Re: [Histonet] Devasting news on 88305TCcomponent
> 
> That is devastating! Do you have a link to this information?
> 
> Sent from my iPhone
> 
> On Nov 2, 2012, at 4:53 AM, "Webster, Thomas S." wrote:
> 
>> Devastating I meant
>> 
>> 
>> CONFIDENTIALITY NOTICE:
>> This e-mail message, including all attachments, is for the sole use
> of the
>> intended recipient(s) and may contain confidential and privileged
>> information. You may NOT use, disclose, copy or disseminate this
>> information. If you are not the intended recipient, please contact
> the
>> sender by reply e-mail immediately. Please destroy all copies of the
>> original message and all attachments. Your cooperation is greatly
>> appreciated.
>> Columbus Regional Hospital
>> 2400 East 17th Street
>> Columbus, Indiana
> 47201___
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
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[Histonet] ALK Positive lung slides

2012-11-02 Thread Johns, Jill
Does anybody have any lung tissue slides that are known to be positive for the 
ALK gene rearrangement or know of a source to obtain them? We are trying to 
validate this FISH assay are having a very difficult time finding any positive 
specimens (we have Abbott's positive control slides, but they do not look like 
"real" tissue specimens)Thanks!!

Jill A. Johns, MT(ASCP)SH, QCym, CCy
Manager of Flow Cytometry and Molecular Diagnostics
Central PA Alliance Laboratory (CPAL)
1803 Mt. Rose Ave., Suite C3/C4
York,  PA17403
phone: (717) 851-4320
fax:  (717) 851-1450
email:  jjoh...@cpallab.com


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[Histonet] Control tissue

2012-11-02 Thread Deloris Carter
Hi,
Can anyone tell me if the control blocks we use for special stains and IHC
need to have the accession number of the original case they came from on
them?  Does this info have to be put on the slides?  Are there any CAP
regulations about this?  Thank you so much for your help.
Deloris Carter HT(ASCP)
Shawnee Mission Medical Center
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Re: [Histonet] RNA isolation form stained slides

2012-11-02 Thread Mark Tarango
Have you tried using more sections during extraction?  Can you extract into
a smaller volume?

On Friday, November 2, 2012, Vanessa Orsini wrote:

>
>
>
>
>
>
> Hello,
>
> I need to extract RNA for a RT-PCR after Laser Micro
> Dissection on xGal stained slides.
>
> I tried using sections from unfixed frozen organs. I fixed the
> sections in EtOH70% for 10min and then I stained them with xGal for 3h at
> 37°C.
> After air drying I cut out with the LCM and extract RNA with the PicoPure
> kit
> from Applied Biosystem. So far I didn’t manage to get enough RNA.
>
> I tried to add RNase inhibitors to all the solutions but it
> didn’t help.
>
>
>
> Any idea/suggestion?
>
> Do someone think it would be better to do a LacZ antibody staining
> on FFPE sections and extract RNA with an appropriate kit? The RNase would
> they
> be less active?
>
>
>
> Thank in advance for any help you can give me J Vanessa
>
>
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Re: [Histonet] Devasting news on 88305TC component

2012-11-02 Thread Brendal Finlay
http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_2013_physician_fee_schedule.html&_state=maximized&_pageLabel=cntvwr


Brendal Finlay, HT (ASCP)
Medical Center Clinic
brendal.fin...@medicalcenterclinic.com
850.474.8758
http://medicalcenterclinic.com
-Original message-
From: Davide Costanzo pathloc...@gmail.com
Date: Fri, 02 Nov 2012 10:09:18 -0500
To: "Webster, Thomas S." twebs...@crh.org
Subject: Re: [Histonet] Devasting news on 88305TCcomponent

That is devastating! Do you have a link to this information?

Sent from my iPhone

On Nov 2, 2012, at 4:53 AM, "Webster, Thomas S." wrote:

> Devastating I meant
>
>
> CONFIDENTIALITY NOTICE:
> This e-mail message, including all attachments, is for the sole use
of the
> intended recipient(s) and may contain confidential and privileged
> information. You may NOT use, disclose, copy or disseminate this
> information. If you are not the intended recipient, please contact
the
> sender by reply e-mail immediately. Please destroy all copies of the
> original message and all attachments. Your cooperation is greatly
> appreciated.
> Columbus Regional Hospital
> 2400 East 17th Street
> Columbus, Indiana
47201___
> Histonet mailing list
>Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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Re: [Histonet] Devasting news on 88305TC component

2012-11-02 Thread Davide Costanzo
That is devastating! Do you have a link to this information?

Sent from my iPhone

On Nov 2, 2012, at 4:53 AM, "Webster, Thomas S."  wrote:

> Devastating I meant
>
>
> CONFIDENTIALITY NOTICE:
> This e-mail message, including all attachments, is for the sole use of the
> intended recipient(s) and may contain confidential and privileged
> information. You may NOT use, disclose, copy or disseminate this
> information.  If you are not the intended recipient, please contact the
> sender by reply e-mail immediately.  Please destroy all copies of the
> original message and all attachments. Your cooperation is greatly
> appreciated.
> Columbus Regional Hospital
> 2400 East 17th Street
> Columbus, Indiana 47201___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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Re: [Histonet] Softening toenails procedure

2012-11-02 Thread Rene J Buesa
Under separate cover I am sending something I wrote about this topic.
René J.



From: Nicole Tatum 
To: histonet@lists.utsouthwestern.edu 
Sent: Friday, November 2, 2012 8:05 AM
Subject: [Histonet] Softening toenails procedure

Hi,

I need a good procedure for softening toe nails to run on H&E. I have used
Nair and KOH.  Anyone have a good procedure that works well that they
would like to share.

Thanks,
Nicole Tatum HT, ASCP


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[Histonet] Free Diagnostic Pathology Software from the UK - Cancer reporting proformas added

2012-11-02 Thread Dr Mayall
Some of the list members might be interested in some recent 
enhancements to the free diagnostic pathology software that we have 
developed in the UK. We are adding proformas for complex cancer cases 
based on the The International Collaboration on Cancer Reporting (ICCR) 
proformas that are being developed. You can trial this free software 
(version 2.1) and download it from this website:


www.freedp.org

Many histopathology laboratories in the UK are using antiquated 
reporting software. Upgrading to a more modern system is expensive. Even 
the more modern systems often lack the technology needed for 
pathologists to
efficiently report complex cases. They are often difficult to use 
across multiple sites. We have developed reporting software that 
overcomes some of these difficulties. Over the last year we have shared 
this open source software internationally via the website above and 
there are now hundreds of users around the World.


Over the next year we are planning to incorporate more cancer 
proformas, and will release an updated version of the software in early 
2013, via the website above.


I would be very for any feed back regarding bugs or ideas for 
enhancements.



Fred Mayall
Consultant Histopathologist Taunton and Somerset NHS Trust







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[Histonet] Softening toenails procedure

2012-11-02 Thread Nicole Tatum
Hi,

I need a good procedure for softening toe nails to run on H&E. I have used
Nair and KOH.  Anyone have a good procedure that works well that they
would like to share.

Thanks,
Nicole Tatum HT, ASCP


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[Histonet] Devasting news on 88305TC component

2012-11-02 Thread Webster, Thomas S.
Devastating I meant


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appreciated.
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[Histonet] Devasting news on 88305TC component

2012-11-02 Thread Webster, Thomas S.
It was cut 52%. I cannot believe this.


http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=statline%2Fspecial_report_final_2013_physician_fee_schedule.html&_state=maximized&_pageLabel=cntvwr


CONFIDENTIALITY NOTICE:
This e-mail message, including all attachments, is for the sole use of the
intended recipient(s) and may contain confidential and privileged
information. You may NOT use, disclose, copy or disseminate this
information.  If you are not the intended recipient, please contact the
sender by reply e-mail immediately.  Please destroy all copies of the
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appreciated.
Columbus Regional Hospital
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[Histonet] RNA isolation form stained slides

2012-11-02 Thread Vanessa Orsini






Hello,

I need to extract RNA for a RT-PCR after Laser Micro
Dissection on xGal stained slides.

I tried using sections from unfixed frozen organs. I fixed the
sections in EtOH70% for 10min and then I stained them with xGal for 3h at 37°C.
After air drying I cut out with the LCM and extract RNA with the PicoPure kit
from Applied Biosystem. So far I didn’t manage to get enough RNA. 

I tried to add RNase inhibitors to all the solutions but it
didn’t help.

 

Any idea/suggestion?

Do someone think it would be better to do a LacZ antibody staining
on FFPE sections and extract RNA with an appropriate kit? The RNase would they
be less active?

 

Thank in advance for any help you can give me J Vanessa

  
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