[Histonet] Pathology requisitions
Our facility, as many do now, uses electronic physician order entry. We struggle with the pathology and cytology portion of this. We still require the manual requisition to be able to obtain the necessary information like clinical history, specific specimen info, etc. Does anyone have a good electronic solution for the pathology/cytology requisition? Any ideas would be greatly appreciated. Aimee Duddey FirstHealth Moore Regional Hospital Pinehurst, NC 28394 adud...@firsthealth.org 910-715-5286 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] What's your favorite mouse-on mouse detection kit
Hi All, For those of you working in research on animals tissue I'm looking for a consensus of opinions on kits for HRP mouse- on -mouse detection. Would like to avoid biotinylation procedures (i.e.ARK). Thanks, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: What's your favorite mouse-on mouse detection kit
Hey Brett, We use Vector PK-2200. Usually works well. Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_man...@merck.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, March 26, 2013 9:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] What's your favorite mouse-on mouse detection kit Hi All, For those of you working in research on animals tissue I'm looking for a consensus of opinions on kits for HRP mouse- on -mouse detection. Would like to avoid biotinylation procedures (i.e.ARK). Thanks, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HT Position
Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is a multi-physician practice located in Northern Kentucky. Its mission is To provide compassionate, high quality, cost-effective care to pateints with gastrointestinal problems Looking for 2 Full Time HT/HTL's ( 32 hours a week is full time employement at this practice ) * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * Supervisor experience preferred * HT/HTL ASCP Certified * Experience with CLIA and CAP * Experience writing and maintaining policies and procedures * Prior laboratory start up experience is preferred * Ability to work independently Duties include: * Grossing * Embedding * Microtomy * Staining; routine and special stains only * Maintain supply orders and laboratory budget * Ability to be flexible and take on additional duties' as needed * Ability to work independently * Maintenance of laboratory for inspections * Maintenance of quality records Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mh...@miracals.commailto:mh...@miracals.com Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mh...@miracals.commailto:mh...@miracals.commailto:mh...@miracals.com%3cmailto:mh...@miracals.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Batch Controlling for IHC
Hello All, Have you ever had a Pathologist say, if you have one tissue control (placed on the same slide as the patient tissue) per antibody, per patient case, but there are multiple blocks (same tissue type) from the same patient case, that would be batch controlling? Hope that made sense, just wondering if this is something others have dealt with. Thanks, Tim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Acid Fast Controls
We are in need of Acid Fast control blocks. Would anybody be interested in trading? We have very good Hpylori and Amyloid blocks. Thank you. Jason Renz HT(ASCP)/lab safety officer St. Alexius Medical Center Bismarck ND, 58506 (701)530-6733 This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] WT1 overstaining on Benchmark Ultra
Hello Histonet, I have two strange occurrences that I'm trying to get to the bottom of. Running WT1 from Cell Marque on a Benchmark Ultra using iView detection and the recommended protocol. Two specimens (both of which happen to be pleural fluid) severely overstained, even leaving the slide with a brown background stain. This has only happened twice, and the slides were on different positions on different staining runs. They used the same antibody and detection kit, however other pleural fluid slides with WT1 are fine. Other slides that run on the same positions also stain ok. I am repeating the stains on the same tissues to try to repeat this but in the meantime has anyone experienced this condition or have any advice? The only time I've seen this condition before was due to a clogged vortex mixer, but it affected all the slides and is obviously not the case here. Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Control Tissue
Does anyone have a good source for obtaining fresh tissue (formalin fixed) for IHC validation? I would need normal and tumor of various types. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: WT1 overstaining on Benchmark Ultra
I've seen the exact same thing happen...WT-1 antibody from Cell Marque, on Ventana instrumentation. Except we use Ultraview detection. I had 3 WT-1 slides running at the same time. Two were overstained like you describe, one was fine. The repeats were fine. The overstained slides almost look like they had been cooked. Interesting! Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Tuesday, March 26, 2013 11:45 AM To: Histonet Subject: [Histonet] WT1 overstaining on Benchmark Ultra Hello Histonet, I have two strange occurrences that I'm trying to get to the bottom of. Running WT1 from Cell Marque on a Benchmark Ultra using iView detection and the recommended protocol. Two specimens (both of which happen to be pleural fluid) severely overstained, even leaving the slide with a brown background stain. This has only happened twice, and the slides were on different positions on different staining runs. They used the same antibody and detection kit, however other pleural fluid slides with WT1 are fine. Other slides that run on the same positions also stain ok. I am repeating the stains on the same tissues to try to repeat this but in the meantime has anyone experienced this condition or have any advice? The only time I've seen this condition before was due to a clogged vortex mixer, but it affected all the slides and is obviously not the case here. Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Batch Controlling for IHC
Technically, he would be right. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Higgins Sent: Tuesday, March 26, 2013 10:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Batch Controlling for IHC Hello All, Have you ever had a Pathologist say, if you have one tissue control (placed on the same slide as the patient tissue) per antibody, per patient case, but there are multiple blocks (same tissue type) from the same patient case, that would be batch controlling? Hope that made sense, just wondering if this is something others have dealt with. Thanks, Tim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. - This message was secured by ZixCorp(R). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: WT1 overstaining on Benchmark Ultra
I have been experiencing the same random over staining issues with WT1 as well. I use the Benchmark Ultras with Ultraview. It started around the same time as a new lot number changed a few months back. I have tried everything, but being so random it is hard to find a solution. Sheila Herrington, IHC Kelowna General Grade 3, Immunohistochemistry, Histolopathogy Kelowna General Hospital -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Tuesday, March 26, 2013 8:57 AM To: 'Dessoye, Michael J'; Histonet Subject: [Histonet] RE: WT1 overstaining on Benchmark Ultra I've seen the exact same thing happen...WT-1 antibody from Cell Marque, on Ventana instrumentation. Except we use Ultraview detection. I had 3 WT-1 slides running at the same time. Two were overstained like you describe, one was fine. The repeats were fine. The overstained slides almost look like they had been cooked. Interesting! Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Tuesday, March 26, 2013 11:45 AM To: Histonet Subject: [Histonet] WT1 overstaining on Benchmark Ultra Hello Histonet, I have two strange occurrences that I'm trying to get to the bottom of. Running WT1 from Cell Marque on a Benchmark Ultra using iView detection and the recommended protocol. Two specimens (both of which happen to be pleural fluid) severely overstained, even leaving the slide with a brown background stain. This has only happened twice, and the slides were on different positions on different staining runs. They used the same antibody and detection kit, however other pleural fluid slides with WT1 are fine. Other slides that run on the same positions also stain ok. I am repeating the stains on the same tissues to try to repeat this but in the meantime has anyone experienced this condition or have any advice? The only time I've seen this condition before was due to a clogged vortex mixer, but it affected all the slides and is obviously not the case here. Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] eye pathology
Hi Fellow 'Netters- Happy Passover, Easter, Solstice (...first day of Spring (last week, then it snowed!!) Is there a reference lab or facility that takes reference cases for eye pathology, specifically corneas? Do they bill Medicare? Same question regarding brain (autopsy) pathology? Thanks in advance! Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT Tech at a time. 281.852.9457 Office 800.756.3309 Phone Fax ad...@fullstaff.org Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to apn...@fullstaff.org. Please include your name and specialty in the body of the email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ASP 300S Stirrer
Hello Histonet, We have an ASP 300S and have found that the stirrer at the bottom of the retort is not staying in place. It pops off of the base during the initial formalin fill and is not providing agitation throughout the process. Has anyone else with this processor had this issue? Any suggestions? Thank you in advance! Meghan Tucker USDA ARS This electronic message contains information generated by the USDA solely for the intended recipients. Any unauthorized interception of this message or the use or disclosure of the information it contains may violate the law and subject the violator to civil or criminal penalties. If you believe you have received this message in error, please notify the sender and delete the email immediately. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Pathology requisitions
We have an electronic requisition that looks like the paper that we use in some units. The ORs didn't use it because they didn't have good access to computers from each room. But the GI labs and Breast Health used it successfully. A Pathology is ordered and fields pop up that the info is typed into. Two copies of the req prints - one in Histolgy with a bar code and one in the unit that is sent down with the specimen. That has served us well for several years. Unfortunately, with our partnerships with Emory and the upgrade to the system, we have to go back to paper. Maybe, someday.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Duddey, Aimee Sent: Tuesday, March 26, 2013 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology requisitions Our facility, as many do now, uses electronic physician order entry. We struggle with the pathology and cytology portion of this. We still require the manual requisition to be able to obtain the necessary information like clinical history, specific specimen info, etc. Does anyone have a good electronic solution for the pathology/cytology requisition? Any ideas would be greatly appreciated. Aimee Duddey FirstHealth Moore Regional Hospital Pinehurst, NC 28394 adud...@firsthealth.org 910-715-5286 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Pathology requisitions
What system are you using to place these orders in the OR? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, March 26, 2013 2:27 PM To: 'Duddey, Aimee'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Pathology requisitions We have an electronic requisition that looks like the paper that we use in some units. The ORs didn't use it because they didn't have good access to computers from each room. But the GI labs and Breast Health used it successfully. A Pathology is ordered and fields pop up that the info is typed into. Two copies of the req prints - one in Histolgy with a bar code and one in the unit that is sent down with the specimen. That has served us well for several years. Unfortunately, with our partnerships with Emory and the upgrade to the system, we have to go back to paper. Maybe, someday.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Duddey, Aimee Sent: Tuesday, March 26, 2013 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology requisitions Our facility, as many do now, uses electronic physician order entry. We struggle with the pathology and cytology portion of this. We still require the manual requisition to be able to obtain the necessary information like clinical history, specific specimen info, etc. Does anyone have a good electronic solution for the pathology/cytology requisition? Any ideas would be greatly appreciated. Aimee Duddey FirstHealth Moore Regional Hospital Pinehurst, NC 28394 adud...@firsthealth.org 910-715-5286 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 112, Issue 28
Hi Sophia, Your message is many hours old, and hopefully you figured out something already. Maybe this will help next time. What stains were you going to do with these slides? Our list of Stain removers may not be compatible with your desired stain. 1) 1% Acid (HCL), ~20 minutes, depending on hematoxylin blend. Acetic acid solutions will not remove the stain.2) 0.5% periodic acid, 5 minutes3) 5% Chromic acid, 5 minutes (wash water) followed by 1% sodium metabisulfite.4) Potassium permanganate, 5 minutes (wash water) followed by oxyalic acid.5) HIER6) Do not use any percentage of bleach. Bleach destroys proteins plus sections float right off. See a trend? Yeah, we've made our share of mistakes too. We also had several linear stainers. My coworker, Lynn, cut a few inches of polypropylene or nylon strapping (box shipping), bend 1.0 cm of one end 90 degrees, and insert between the container you don't want the slides to fall into + downstream. Put the piece over the top of the front third of the staining containers, to block the slide holders, not the slide bottoms. It saved us many times. Hope all turned out well,HughUH Cancer Center -- Date: Mon, 25 Mar 2013 08:06:22 -0700 From: Sophia Lin lins0...@gmail.com Subject: [Histonet] hematoxylin back to deparaffin? To: histonet@lists.utsouthwestern.edu Message-ID: cajgpzdr5j-sgeya0u7t4mevmtcgxgkvw_vmkwnp7jt99ew3...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 We had a slight mistake with the deparaffin slides. A timer was not set, long story short. The deparaffinized slides went too far and proceeded to Hematoxylin. We have a linear stainer, so we took it back out from hematoxylin when we noticed the error and placed it into water to rinse. Then back to acid alchol. But the hematoxylin is still visible. Any tips? I have taken it back from completel HE, just not hematoxylin... any advice would be great! Recutting isnt a good option. Thanks! -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 112, Issue 28 * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
