[Histonet] Final Week to Register for FSH
Good Afternoon Histonetters! Hotel reservations should be made quickly to ensure rate and availability. After today you will have to hope that room prices will not go up. I am working on trying to squeeze out a few more days for the hotel registration link below. Try this link below first and if it does not work make sure that when you call (1-866-397-6516) the hotel to make reservations that you mention Florida Society for Histotechnology. Act now to save money and possible aggravation. Looking forward to seeing you at our meeting! Meeting Program/agenda http://www.fshgroup.org/wp-content/uploads/2015/03/FSH-2015-Online-Program-revised-6.pdf Hotel online reservation https://reservations.ihotelier.com/crs/g_reservation.cfm?groupID=1251402hotelID=6579 Act quickly!!! Online meeting registration https://www.regonline.com/Register/Checkin.aspx?EventID=1679155lbrd=1rtypeid=380141 Prices go up after tomorrow for registration and workshops! Have a great day! Kind Regards! John Shelley 2014-2016 FSH President ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Program in Interactive tissue microarray and quantitative digital pathology workshop announcement
Dear Histonetters: Pasquale De Blasio asked me to provide you the announcement about a meeting in Rome this June. I copied the content from the meeting flyer below. Unfortunately, the Histonet server would block all the graphics and artwork in the flyer so I could not include that. I have no personal knowledge of him or the meeting. His email is pasquale.debla...@isenet.itmailto:pasquale.debla...@isenet.it if you have questions about this meeting. Thanks. PRELIMINARY PROGRAM INTERACTIVE TISSUE MICROARRAY AND QUANTITATIVE DIGITAL PATHOLOGY WORKSHOP June 8-9th 2015 University of Tor Vergata, Campus X, Rome - Italy On-line registration: https://eventbrite.com/event/16536197211 June 8th, 2015 08:00 - 08:45 REGISTRATION 08:45 - 09:00 WELCOME Remarks - Prof. Luigi Spagnoli, Pasquale De Blasio 09:00 - 11:00 SESSION 1 - TISSUE MICROARRAY TECHNOLOGY - Chair: Pasquale De Blasio - History of Tissue Microarray Technology Ulrich F. Vogel, UKT, Institute of Pathology, Tuebingen, (Germany) - Visualizing and Quantifying Cell Populations from Brightfield and Fluorescent TMA Samples Thomas J. Diefenbach, The Ragon Institute of MGH, MIT and Harvard - Cambridge, MA (USA) - Management of a Pathology TMA and Virtual Image Infrastructure for Research and Diagnostics Peter Riegman, Erasmus MC Cancer Institute, Rotterdam, (The Netherlands) - TMA Technology for: Tissue Microdissection, DNA RNA and protein extract Giorgio Stanta, Department of Medical Sciences, University of Trieste, (Italy) 11:00 - 11:20 Coffee break 11:20 - 13:30 SESSION 2 - PRACTICAL CONSTRUCTION OF A TISSUE ARRAY - Organization of FFPE Archive for TMA Use in Research and Diagnostic Luigi Spagnoli, Professor of Pathology Emeritus, University of Tor Vergata, Rome (Italy) - Tissue and Cell Microarray: A Cross-Over Validation Tool for Stem Cell Research Ida Biunno, Institute of Genetic and Biomedical Research (IRGB-CNR) , Milan (Italy) - Concepts of Quality Assurance and Quality Control in the use of Tissues Paolo Locatelli, Area Manager, Milestone srl, Sorisole - Bergamo (Italy) - TMA Application in Neurodegenerative Disorders Roberto Dominici, Analysis and Diagnostic Laboratory, Abbiategrasso Hospital, Milan (Italy) - Ethical Aspects of use of TMA slides for Research and Diagnostics Speaker to be confirmed 13:30 - 14:30 Lunch Break 14:00 - 17:00 SESSION 3 - HANDS-ON TISSUE ARRAYERS, SCANNERS AND IMAGE ANALYSIS SYSTEMS - Tissue Microarrayer Platform Bring your own Tissue Blocks and make your TMA - Digital Scanners Image Analysis Software (Analyze your TMA Slide) Bring your Tissue and TMA Slide and get them analysed - Visit Exhibition Boots and look at instruments and tools which can enhance your work Tissue Vacuum technology, Digital Scanners, Visual Imaging Software June 9th, 2015 09:00 - 11:00 SESSION 4 - QUANTITATIVE DIGITAL PATHOLOGY IMAGE ANALYSIS - TMA Platform for Biomarkers Validation and Clinical Applications Pasquale De Blasio, Integrated Systems Engineering srl, Milan (Italy) - Automated image analysis in histopathology: a valuable tool in medical diagnostics George Steiner, TissueGnostics, Wien (Austria) - Digital pathology: combining whole slide imaging, multiplex staining and automated image analysis Speaker to be confirmed (Visiopharm) - Proliferation markers and automated tumour detection Speaker to be confirmed (Hamamatsu) 11:30 - 13:00 SESSION 4 - (Cont.) QUANTITATIVE DIGITAL PATHOLOGY IMAGE ANALYSIS - Review of imaging solutions for integrated quantitative immunohistochemistry in the Pathology daily practice Speaker to be confirmed (Leika/Aperio) - Digital pathology: current status and future perspectives Speaker to be confirmed (Dako) - Virtual microscopy and digital pathology in training and education Speaker to be confirmed (PathXL) - Virtual Microscope System Speaker to be confirmed (Olympus) 13:00 - 14:00 Lunch 14:00 - 16:00 SESSION 5 - HANDS-ON TISSUE ARRAYERS, SCANNERS AND IMAGE ANALYSIS SYSTEMS - Tissue Microarrayer Platform Bring your own Tissue Blocks and make your TMA - Digital Scanners Image Analysis Software (Analyze your TMA Slide) Bring your Tissue and TMA Slide and get them analysed - Visit Exhibition Boots and look at instruments and tools which can enhance your work Tissue Vacuum technology, Digital Scanners, Visual Imaging ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Billing question
If I have two sections on an A specimen A1 and A2 and both had a GMS stain, do I charge 1 - 88312 or 2 - 88312? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Billing question
2 - specials are per block.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: Vickroy, James [mailto:jvick...@springfieldclinic.com] Sent: Thursday, May 07, 2015 4:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing question If I have two sections on an A specimen A1 and A2 and both had a GMS stain, do I charge 1 - 88312 or 2 - 88312? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Embedding Center Temperature
Hi, I am curious about which temperatures people are tracking on their embedding centers for CAP, and how they are tracking them. If you are tracking the temps for the forceps and hot and cold plates, are you using the internal thermometer or measuring the temperature another way? Thanks. Elizabeth M. Cameron, HT (ASCP), QIHC Lead Histologist Mid Coast Hospital 123 Medical Center Drive Brunswick, ME 04011 (207) 373-6573 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histonet server issues
Hi, I just saw the messages about there being problems with the Histonet website http://lists.utsouthwestern.edu/mailman/listinfo/histonet The university was indeed doing some work on the server at the beginning of the month and they thought it wouldn't effect users much. I checked and the website seems to be functional now and as far as I can tell, messages are going through as usual. If you have persistent problems, please let me know at the histonet-ow...@lists.utsouthwestern.edumailto:histonet-ow...@lists.utsouthwestern.edu address. Thanks. Marvin Hanna's site that keeps the archive http://www.histosearch.com/histonet.html also seems to be functioning properly. Best regards, Linda M Histonet administrator ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HE Strainers
I currently have a Sakura Tissue TEK Prisma stainer that is 10+ yrs. old and we are now having issues with staining quality. I have recently had the machine maintenance and serviced, but we are looking to purchase a new stainer . Anyone have any recommendations, or any particular instrument or vendor they wouldn't work with? Anyone work with the new Roche system that eliminates xylene and alcohol? Any feedback would be greatly appreciated. Cindy Bird ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histonet links
Histonet links appear to be functioning at a less-than-optimal level, but here they are anyway. I can almost 'feel the pain' afflicting Linda and Anita, but, of course, that's a pompous, maladjusted, insensitive, and politically correct lie. http://lists.utsouthwestern.edu/mailman/listinfo/histonet Histonethttp://lists.utsouthwestern.edu/mailman/listinfo/histonet list run by lindamargraf at gmail.com, anita.sengupta at childrens.commailto:histonet-ow...@lists.utsouthwestern.edu The above work, but I have NOT tried the emails. Just couldn't do it. If the first doesn't work, then, perhaps the 'owners' are having a problem or two. Cheers, Fred Monson P.S. The one thing that Darwinian Evolution must have is MAXIMUM variation, so get illuminated, and B_E_E_E_EG_O_O_O_D_D_D! Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of Pennsylvania Schmucker Science South - Room SSS-024 MailDrop: Geology-Astronomy 750 South Church Street West Chester, PA, 19383 610-738-0437 fmon...@wcupa.edumailto:fmon...@wcupa.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Still having Issues with Acetone fixation.
Hi everyone, Sorry to keep posting about this, But I am still having Acetone issues. I am doing IHC for Cell surface markers that are lost when fixing with etoh or methanol. When I fix in 100% acetone my epitopes have great signal. However, when I fix in 100% acetone, my tissues all damaged by the acetone beyond all recognition. I can lose up to 90% of my tissue when I fix in 100% acetone for 10 minutes. But, I get good epitope staining if I have some tissue left on the slide. When I fix in anything else, I lose 90% or more of my epitope staining, but my tissue morphology looks great. -- What's the least amount of time I can fix in 100% Acetone for a 5uM section and still have it be fixed? Is drying after the 100% acetone fixing essential? or Bad for protecting tissue morphology? -- I am doing IHC on human tonsils cut to 5 uM with a 24 dry after sectioning. When I fix in 100% acetone, I fix it at 4C for 10 minutes. Then dry for 1 hour in the fume hood Should it go straight into buffer? Should it be for less time in the acetone? Should the acetone be Room temp or -20C instead of 4C. If I was diluting the acetone with buffer, (or etoh) then I could see going straight into buffer afterwards, but because I am using 100% I think that going into liquid right after fixing is too much of a change and my tissues go BOOM. Please help. Patrick Lewis Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histonet Digest, Vol 138, Issue 9
For some reason histonet is being delivered blank. -Original Message- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: Thursday, May 07, 2015 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 138, Issue 9 Confidentiality Notice: This email message and any attachments from Bay Medical Center Sacred Heart Health System, its subsidiaries and affiliates, are confidential and for the sole use of the intended recipient. This communication may contain privileged, proprietary, or confidential information (i.e., including Protected Health Information), which may only be used or disclosed in accordance with applicable law. If you are not the intended recipient of this email or the employee or agent responsible for delivering the communication to the intended recipient, then you may not read, copy, distribute or otherwise use or disclose the information contained in this message. If you received this message in error, please notify us by an email to postmas...@baymedical.org. Please indicate that you were not the intended recipient, and confirm that you have deleted the original message and any attachments as well as any printed copies. Please do not retransmit the contents of the message. T hank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
