Re: [Histonet] Billing question

2015-05-07 Thread Weems, Joyce K.
2 - specials are per block..

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



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-Original Message-
From: Vickroy, James [mailto:jvick...@springfieldclinic.com]
Sent: Thursday, May 07, 2015 4:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Billing question

If I have two sections on an "A" specimen A1 and A2 and both had a GMS stain, 
do I charge 1 - 88312 or  2 - 88312?

Jim

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com



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[Histonet] Billing question

2015-05-07 Thread Vickroy, James
If I have two sections on an "A" specimen A1 and A2 and both had a GMS stain, 
do I charge 1 - 88312 or  2 - 88312?

Jim

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com



This electronic message contains information from Springfield Clinic, LLP that 
may be confidential, privileged, and/or sensitive. This information is intended 
for the use of the individual(s) or entity(ies) named above. If you are not the 
intended recipient, be aware that disclosure, copying, distribution, or action 
taken on the contents of this information is strictly prohibited. If you have 
received this electronic message in error, please notify the sender 
immediately, by electronic mail, so that arrangements may be made for the 
retrieval of this electronic message. Thank you.
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[Histonet] Program in Interactive tissue microarray and quantitative digital pathology workshop announcement

2015-05-07 Thread Linda Margraf
Dear Histonetters:



Pasquale De Blasio asked me to provide you the announcement about a meeting in 
Rome this June.  I copied the content from the meeting flyer below. 
Unfortunately,  the Histonet server would block all the graphics and artwork in 
the flyer so I could not include that.   I have no personal knowledge of him or 
the meeting. His email is  
pasquale.debla...@isenet.it if you have 
questions about this meeting. Thanks.







PRELIMINARY PROGRAM  INTERACTIVE TISSUE MICROARRAY AND QUANTITATIVE DIGITAL 
PATHOLOGY WORKSHOP

June 8-9th 2015 University of "Tor Vergata", Campus X, Rome - Italy

On-line registration: https://eventbrite.com/event/16536197211



June 8th, 2015

08:00 - 08:45 REGISTRATION

08:45 - 09:00 WELCOME Remarks - Prof. Luigi Spagnoli, Pasquale De Blasio

09:00 - 11:00 SESSION 1 - TISSUE MICROARRAY TECHNOLOGY - Chair: Pasquale De 
Blasio
- History of Tissue Microarray Technology
Ulrich F. Vogel, UKT, Institute of Pathology, Tuebingen, (Germany)
- Visualizing and Quantifying Cell Populations from Brightfield and Fluorescent 
TMA Samples
Thomas J. Diefenbach, The Ragon Institute of MGH, MIT and Harvard - Cambridge, 
MA (USA)
- Management of a Pathology TMA and Virtual Image Infrastructure for Research 
and Diagnostics
Peter Riegman, Erasmus MC Cancer Institute, Rotterdam, (The Netherlands)

- TMA Technology for: Tissue Microdissection, DNA & RNA and protein extract
Giorgio Stanta, Department of Medical Sciences, University of Trieste, (Italy)

11:00 - 11:20 Coffee break

11:20 - 13:30 SESSION 2 - PRACTICAL CONSTRUCTION OF A TISSUE ARRAY
- Organization of FFPE Archive for TMA Use in Research and Diagnostic
Luigi Spagnoli, Professor of Pathology Emeritus, University of Tor Vergata, 
Rome (Italy)
- Tissue and Cell Microarray: A Cross-Over Validation Tool for Stem Cell 
Research
Ida Biunno, Institute of Genetic and Biomedical Research (IRGB-CNR) , Milan 
(Italy)
- Concepts of Quality Assurance and Quality Control in the use of Tissues
Paolo Locatelli, Area Manager, Milestone srl, Sorisole - Bergamo (Italy)
- TMA Application in Neurodegenerative Disorders
Roberto Dominici, Analysis and Diagnostic Laboratory, Abbiategrasso Hospital, 
Milan (Italy)
- Ethical Aspects of use of TMA slides for Research and Diagnostics
Speaker to be confirmed

13:30 - 14:30 Lunch Break

14:00 - 17:00 SESSION 3 - HANDS-ON TISSUE ARRAYERS, SCANNERS AND IMAGE ANALYSIS 
SYSTEMS
- Tissue Microarrayer Platform
Bring your own Tissue Blocks and make your TMA
- Digital Scanners & Image Analysis Software (Analyze your TMA Slide)
Bring your Tissue and TMA Slide and get them analysed
- Visit Exhibition Boots and look at instruments and tools which can enhance 
your work
Tissue Vacuum technology, Digital Scanners, Visual Imaging Software



June 9th, 2015

09:00 - 11:00 SESSION 4 - QUANTITATIVE DIGITAL PATHOLOGY IMAGE ANALYSIS

- TMA Platform for Biomarkers Validation and Clinical Applications

Pasquale De Blasio, Integrated Systems Engineering srl, Milan (Italy)

- Automated image analysis in histopathology: a valuable tool in medical 
diagnostics

George Steiner, TissueGnostics, Wien (Austria)

- Digital pathology: combining whole slide imaging, multiplex staining and 
automated image analysis

Speaker to be confirmed (Visiopharm)

- Proliferation markers and automated tumour detection

Speaker to be confirmed (Hamamatsu)



11:30 - 13:00 SESSION 4 - (Cont.) QUANTITATIVE DIGITAL PATHOLOGY IMAGE ANALYSIS

- Review of imaging solutions for integrated quantitative immunohistochemistry 
in the Pathology daily practice

Speaker to be confirmed (Leika/Aperio)

- Digital pathology: current status and future perspectives

Speaker to be confirmed (Dako)

- Virtual microscopy and digital pathology in training and education

Speaker to be confirmed (PathXL)

- Virtual Microscope System

Speaker to be confirmed (Olympus)

13:00 - 14:00 Lunch



14:00 - 16:00 SESSION 5 - HANDS-ON TISSUE ARRAYERS, SCANNERS AND IMAGE ANALYSIS 
SYSTEMS

- Tissue Microarrayer Platform

Bring your own Tissue Blocks and make your TMA

- Digital Scanners & Image Analysis Software (Analyze your TMA Slide)

Bring your Tissue and TMA Slide and get them analysed

- Visit Exhibition Boots and look at instruments and tools which can enhance 
your work
Tissue Vacuum technology, Digital Scanners, Visual Imaging

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[Histonet] Final Week to Register for FSH

2015-05-07 Thread John Shelley
Good Afternoon Histonetters!

Hotel reservations should be made quickly to ensure rate and availability. 
After today you will have to hope that room prices will not go up. I am working 
on trying to squeeze out a few more days for the hotel registration link below. 
Try this link below first and if it does not work make sure that when you call 
(1-866-397-6516) the hotel to make reservations that you mention Florida 
Society for Histotechnology.  Act now to save money and possible aggravation. 
Looking forward to seeing you at our meeting!

Meeting Program/agenda 
http://www.fshgroup.org/wp-content/uploads/2015/03/FSH-2015-Online-Program-revised-6.pdf

Hotel online reservation  
https://reservations.ihotelier.com/crs/g_reservation.cfm?groupID=1251402&hotelID=6579
  Act quickly!!!

Online meeting registration 
https://www.regonline.com/Register/Checkin.aspx?EventID=1679155&lbrd=1&rtypeid=380141
 Prices go up after tomorrow for registration and workshops!

Have a great day!

Kind Regards!

John Shelley
2014-2016 FSH President

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Re: [Histonet] Histonet Digest, Vol 138, Issue 9

2015-05-07 Thread Myra Huth
For some reason histonet is being delivered blank.

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From: histonet-requ...@lists.utsouthwestern.edu 
[mailto:histonet-requ...@lists.utsouthwestern.edu]
Sent: Thursday, May 07, 2015 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 138, Issue 9


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[Histonet] Still having Issues with Acetone fixation.

2015-05-07 Thread Lewis, Patrick
Hi everyone,

Sorry to keep posting about this,

But I am still having Acetone issues.

I am doing IHC for Cell surface markers that are lost when fixing with etoh or 
methanol.

When I fix in 100% acetone my epitopes have great signal.

However,  when I fix in 100% acetone, my tissues all damaged by the acetone 
beyond all recognition.

I can lose up to 90% of my tissue when I fix in 100% acetone for 10 minutes.

But, I get good epitope staining if I have some tissue left on the slide.

When I fix in anything else, I lose 90% or more of my epitope staining, but my 
tissue morphology looks great.

--
What's the least amount of time I can fix in 100% Acetone for a 5uM section and 
still have it be fixed?

Is drying after the 100% acetone fixing essential? or Bad for protecting tissue 
morphology?

--
I am doing IHC on human tonsils cut to 5 uM with a 24 dry after sectioning.

When I fix in 100% acetone, I fix it at 4C for 10 minutes. Then dry for 1 hour 
in the fume hood

Should it go straight into buffer? Should it be for less time in the acetone?
Should the acetone be Room temp or -20C instead of 4C.

If I was diluting the acetone with buffer, (or etoh) then I could see going 
straight into buffer afterwards, but because I am using 100% I think that going 
into liquid right after fixing is too much of a change and my tissues go BOOM.

Please help.

Patrick Lewis


Patrick Lewis
Research Associate II Bench
Seattle Childrens Research Institute
206-884-1115

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[Histonet] Histonet links

2015-05-07 Thread Monson, Frederick
Histonet links appear to be functioning at a less-than-optimal level, but here 
they are anyway.
I can almost 'feel the pain' afflicting Linda and Anita, but, of course, that's 
a pompous, maladjusted, insensitive, and politically correct lie.

http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Histonet list run by 
lindamargraf at gmail.com, anita.sengupta at 
childrens.com

The above work, but I have NOT tried the emails.  Just couldn't do it.

If the first doesn't work, then, perhaps the 'owners' are having  a problem or 
two.

Cheers,

Fred Monson

P.S.  The one thing that Darwinian Evolution must have is MAXIMUM variation, so 
get illuminated, and "B_E_E_E_EG_O_O_O_D_D_D!"

Frederick C. Monson, PhD
Technical Director
Center for Microanalysis and Imaging, Research and Training (CMIRT)
West Chester University of Pennsylvania
Schmucker Science South - Room SSS-024
MailDrop:  Geology-Astronomy
750 South Church Street
West Chester, PA, 19383
610-738-0437
fmon...@wcupa.edu

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[Histonet] H&E Strainers

2015-05-07 Thread Cindy Bird
I currently have a Sakura Tissue TEK Prisma stainer that is 10+ yrs. old and we 
are now having issues with staining quality.  I have recently had the machine 
maintenance and serviced, but we are looking to purchase a new stainer .  
Anyone have any recommendations, or any particular instrument or vendor they 
wouldn't work with?  Anyone work with the new Roche system that eliminates 
xylene and alcohol?

Any feedback would be greatly appreciated.

Cindy Bird

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[Histonet] Histonet server issues

2015-05-07 Thread Linda Margraf
Hi, I just saw the messages about there being problems with the Histonet 
website http://lists.utsouthwestern.edu/mailman/listinfo/histonet
The university was indeed doing some work on the server at the beginning of the 
month and they thought it wouldn't effect users much.   I checked and the 
website seems to be functional now and as far as I can tell, messages are going 
through as usual.   If you have persistent problems, please let me know at the 
histonet-ow...@lists.utsouthwestern.edu
 address.  Thanks.

Marvin Hanna's site that keeps the archive 
http://www.histosearch.com/histonet.html   also seems to be functioning 
properly.

Best regards,
Linda M
Histonet administrator

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[Histonet] Embedding Center Temperature

2015-05-07 Thread Cameron, Elizabeth
Hi,
I am curious about which temperatures people are tracking on their embedding 
centers for CAP, and how they are tracking them.  If you are tracking the temps 
for the forceps and hot and cold plates, are you using the internal thermometer 
or measuring the temperature another way?
Thanks.

Elizabeth M. Cameron, HT (ASCP), QIHC
Lead Histologist
Mid Coast Hospital
123 Medical Center Drive
Brunswick, ME 04011
(207) 373-6573

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