[Histonet] Symphony users

[Histology Technician via Histonet]

 Symphony users, can you message me?  I want to talk about prices, what you 
like/dislike about it off line.  Thanks!
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[Histonet] A blogpost for parents, or the children of parents.

[Lester Raff MD via Histonet]

Hello Netters---

Something to bring a smile to  your "leisure" time.

Click here for blog   Read this one, because we have 
all been there!

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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[Histonet] LFABP and PHH3 Abs

[Kiranjit Grewal via Histonet]

Dear Experts,

We are trying to optimize LFABP( Liver marker) and PHH3( Mitosis marker). 
Anyone willing to share their protocol or suggestions that can help expedite 
the process is much appericiated. We have both Leica & Ventana instruments.

thank you in advance!

Kiran

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[Histonet] Paraffin sectioning for DNA analysis

[P Sicurello via Histonet]

Hello Listers,



Which techniques are you all using to avoid cross contamination when
cutting multiple sections from multiple blocks for DNA analysis?



Way back I used to use DEP-C treated water, wore gloves, changed the blade
between blocks and wiped down the surface of the knife holder plate with
the DEP-C water and ETOH.



What is the currently accepted procedure?



Sincerely,

Paula


Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872



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[Histonet] Pas Digestion

[Lager, Loree via Histonet]

Hello,

This is my first time using the histonet, as our veteran user retired.

We've been having an intermittent problem with incomplete glycogen digestion on 
PASD.  Our digestion solution is 0.5%  α-Amylase, Type VI-B from porcine 
pancreas (Sigma), which we freeze in small aliquots and thaw for 45 minutes at 
room temperature before use. We digest for 20 minutes @ room temperature, 
dropping amylase on slides.

In troubleshooting the issue, we discovered amylase was several years old, so 
new amylase ordered.   The new lot (while the same product number) produced 
even worse results.  After a closer look we saw that the new lot contained ½ of 
the units/mg solid, 26 to 13, not sure why?

Anyone willing to share their procedure or suggestions to help us resolve this 
issue?

Thank you,
Loree Lager
Seattle Children's Hospital
Histology




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Re: [Histonet] Pas Digestion

[Jennifer MacDonald via Histonet]

The optimal temperature for most enzymes in 37 degrees Celsius. 




From:   "Lager, Loree via Histonet" 
To: "'histonet@lists.utsouthwestern.edu'" 

Date:   11/03/2015 12:38 PM
Subject:[Histonet] Pas Digestion



Hello,

This is my first time using the histonet, as our veteran user retired.

We've been having an intermittent problem with incomplete glycogen 
digestion on PASD.  Our digestion solution is 0.5%  α-Amylase, Type VI-B 
from porcine pancreas (Sigma), which we freeze in small aliquots and thaw 
for 45 minutes at room temperature before use. We digest for 20 minutes @ 
room temperature, dropping amylase on slides.

In troubleshooting the issue, we discovered amylase was several years old, 
so new amylase ordered.   The new lot (while the same product number) 
produced even worse results.  After a closer look we saw that the new lot 
contained ½ of the units/mg solid, 26 to 13, not sure why?

Anyone willing to share their procedure or suggestions to help us resolve 
this issue?

Thank you,
Loree Lager
Seattle Children's Hospital
Histology




CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is 
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and privileged information protected by law. Any unauthorized review, use, 
disclosure or distribution is prohibited. If you are not the intended 
recipient, please contact the sender by reply e-mail and destroy all 
copies of the original message.
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Re: [Histonet] Pas Digestion

[Ray via Histonet]

As Jennifer mentioned, enzymes have optimal temperatures at which to work.  But 
also optimal pH.  Is your 0.5% amylase in water or a correct buffer? 
Ray, Seattle, WA 

- Original Message -

From: "Loree via Histonet Lager"  
To: "histonet@lists.utsouthwestern.edu"  
Sent: Tuesday, November 3, 2015 12:37:04 PM 
Subject: [Histonet] Pas Digestion 

Hello, 

This is my first time using the histonet, as our veteran user retired. 

We've been having an intermittent problem with incomplete glycogen digestion on 
PASD.  Our digestion solution is 0.5%  α-Amylase, Type VI-B from porcine 
pancreas (Sigma), which we freeze in small aliquots and thaw for 45 minutes at 
room temperature before use. We digest for 20 minutes @ room temperature, 
dropping amylase on slides. 

In troubleshooting the issue, we discovered amylase was several years old, so 
new amylase ordered.   The new lot (while the same product number) produced 
even worse results.  After a closer look we saw that the new lot contained ½ of 
the units/mg solid, 26 to 13, not sure why? 

Anyone willing to share their procedure or suggestions to help us resolve this 
issue? 

Thank you, 
Loree Lager 
Seattle Children's Hospital 
Histology 




CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for 
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privileged information protected by law. Any unauthorized review, use, 
disclosure or distribution is prohibited. If you are not the intended 
recipient, please contact the sender by reply e-mail and destroy all copies of 
the original message. 
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[Histonet] (no subject)

[Eck, Allison via Histonet]

How many of you have a cytology dept where your cyto techs do all their own 
prep and reports?  If you don't, do you have a cyto prep aide or does a histo 
tech help?

Also, how many of you have histo and cyto as the same department with an AP 
manager?  Does the AP manager oversee transcription as well

Thank you in advance



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[Histonet] FW: A blogpost for parents, or the children of parents.--problem with link??

[Lester Raff MD via Histonet]

If the previous link did not work in your browser, try the full address

http://www.chicagonow.com/downsize-maybe/2015/11/if-you-are-a-parent-or-have-ever-had-one-the-forever-plan-is-for-you/

Read, comment, subscribe

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

-Original Message-
From: Lester Raff MD via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, November 03, 2015 9:15 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] A blogpost for parents, or the children of parents.

Hello Netters---

Something to bring a smile to  your "leisure" time.

Click here for blog   Read this one, because we have 
all been there!

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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[Histonet] CD8 IHC on FFPE mouse tissue -what your experience?

[Connolly, Brett M via Histonet]

Hi all,
I have a project that will be looking at CD8 expression in some FFPE murine 
tissue.  Past discussions here have mentioned the 14-0808 CD8a antibody from 
eBiosicence.

For those of you have had the opportunity to use this antibody I would like to 
hear your experiences/ recommendations about the staining results.

I'll need to do some quantitation of the staining so I am hoping this antibody 
is strong and specific.

Thanks much,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

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[Histonet] Acid hematin

[raestask--- via Histonet]

I saw a Histonet post that Peggy Wenk had made concerning the recipe for 
alcoholic formalin for processing. She noted that you use the solution 
unbuffered.  How do you control acid hematin pigment in such a solution? 
We use a commercial alcoholic formalin “concentrate” that is diluted with 
alcohol and water prior to use.  It is supposedly buffered.  We have great 
problems with acid hematin.  Do you have any suggestions?  For preprocessing 
fixation, we make our own buffered formalin from scratch or from a concentrate 
and get biopsy specimens from veterinarians fixed in who-knows-what. 
Thank you very much, 
DW 
  
Card
  

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Re: [Histonet] CD8 IHC on FFPE mouse tissue -what your experience?

[Colleen Forster via Histonet]

I am interested to see what you hear BrettI need to do these same
markers and am not having good luck in FFPEfrozens , great...FFPE NOT!

C

On Tue, Nov 3, 2015 at 7:32 AM, Connolly, Brett M via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hi all,
> I have a project that will be looking at CD8 expression in some FFPE
> murine tissue.  Past discussions here have mentioned the 14-0808 CD8a
> antibody from eBiosicence.
>
> For those of you have had the opportunity to use this antibody I would
> like to hear your experiences/ recommendations about the staining results.
>
> I'll need to do some quantitation of the staining so I am hoping this
> antibody is strong and specific.
>
> Thanks much,
> Brett
>
> Brett M. Connolly, Ph.D.
> Prin. Scientist,
> Translational Biomarkers - Imaging
> Merck & Co., Inc.
> PO Box 4, WP-44K
> West Point, PA 19486
> brett_conno...@merck.com
> T- 215-652-2501
> F- 215-993-6803
>
> Notice:  This e-mail message, together with any attachments, contains
> information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
> New Jersey, USA 07033), and/or its affiliates Direct contact information
> for affiliates is available at
> http://www.merck.com/contact/contacts.html) that may be confidential,
> proprietary copyrighted and/or legally privileged. It is intended solely
> for the use of the individual or entity named on this message. If you are
> not the intended recipient, and have received this message in error,
> please notify us immediately by reply e-mail and then delete it from
> your system.
> ___
> Histonet mailing list
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>
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