Re: [Histonet] plant tissue stain

[Rene J Buesa via Histonet]

I always used a green (Fast green) for cell walls and cytoplasm + a red 
(fuchsine) for nuclei.If you get Peter Gray's book you will find numerous plant 
procedures. In Bolles-Lee (Microtomist's Vade-Mecum) there are also many 
methods for plant tissues.René 


 On Saturday, November 7, 2015 6:13 PM, Peter Noyce via Histonet 
 wrote:
   

 This may seem a bit odd for this forum but what is the best stain for plant
tissue histology slides-to show nuclei, cytoplasm, cell wall.

PW Noyce.

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Re: [Histonet] Competency Assessments

[Rene J Buesa via Histonet]

Elaine:As you wrote there are differences of opinion, so here is mine:Start 
with "analysis" which is the process of determining the qualities of something. 
As I see it, in histology the pathologist is the one who analyzes = determines 
the qualities of the tissue sections and gets to a diagnosis.Consequently, 
pre-analytical are all those steps leading to the preparation of the slide, 
namely, grossing, processing, sectioning and staining.The PT analyzes the 
finished tissue section and asks for special procedures; and those are post 
analytical but they can be also considered as part of a final diagnoses and, as 
such, are also "pre-analytical". If you take this last definition, then the 
post analytical will be limited to everything is done in the lab after the 
diagnosis is reached and will include billing, contacting the patient/referring 
physician, archiving and other tasks.So you have first to get CAP's definition 
or elaborate your own one in accordance with your lab director.René 


 On Saturday, November 7, 2015 2:36 PM, Elaine allison Hoffman via Histonet 
 wrote:
   

 Greeting everyone in Histo-land,
I'm still working on a competency assessment procedure but I have a question 
that needs clarification.  According to CAP, "A laboratory must evaluate and 
document the competency of all testing personnel for each test system.  A TEST 
SYSTEM is the process that includes pre-analytic, analytic, and post-analytic 
steps used to produce a test result or set of results.  A test system may be 
manual, automated, multi-channel or single use and can include reagents, 
components, equipment or instruments required to produce results".  My question 
is, what histology tasks or steps are considered pre-analytic, analytic, and 
post-analytic?  In all the resources I've found, there seems to be a difference 
of opinion as to what is considered pre-analytic, analytic, and post-analytic 
specific to histology tasks which is not really considered "resulting" in 
histology. Also, there should be a different TEST SYSTEM for what pathologists 
do (resulting slides) and what PA's do (gross description of tissue), so what 
tasks are considered pre-analytic, analytic, and post-analytic specific for 
their jobs? A person could go crazy with all this and to ask an inspector, they 
aren't really sure either.  And different inspectors will give you different 
answers.Any takers out there?
Elaine Hoffman
    
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