Re: [Histonet] Duraedge (Black) help

[Rene J Buesa via Histonet]

Extremely rare this problem because almost always thick/thin sections has 
nothing to do with the blades but with the microtome that is unable to hold the 
blade firmly.Are the blades in this microtome "thinner" than the others used in 
it previously?If they are, you could add a small piece of aluminum foil 
surrounding the thinner blade to make it thicker and perhaps enabling the blade 
holder in this microtome to hold them tighter. Just a thought.René 


On Monday, November 30, 2015 11:54 AM, Hans B Snyder via Histonet 
 wrote:
 

 Hello All,

I recently switched from Duraedge (red) to Duraedge (black) blades.  This
has caused cutting problems on 1 microtome, our Leica RM 2235 microtome but
no others.  The sections are now thick and thin, compressed or lengthwise
ribbon splits.

I tried changing the blade holder angle, 6 forward and 6 back positions
with no luck.  After trying 12 different knife angles with no luck, I had
someone PM it and it's still not working. The original red blades still
work in the microtome and I don't understand how only 1 microtome does not
work with these blades.  Instead of buying a whole new case of red blades
just for this 1 microtome, I thought you might have some insight that could
help.

What should I do?

Thank you

Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
h...@histologistics.com 

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[Histonet] Leica Bond Detection Kits

[Sheila Fonner via Histonet]

 Hello everyone, hope this Monday is treating you well. I am in dire need of 
some assistance. We are a dermpath  lab in Knoxville, TN using the Leica Bond 
for our IHC. We have been unable to acquire our Refine Red Detection Kits due 
to some technical difficulty. I am looking for anyone who currently uses the 
refine red and would be willing and/or able to loan us 2 red kits until we 
receive our new lot. I have 9 on order but I am extremely backed up. We would 
pay for all shipping and would replace them asap. Thank you for any help you 
can provide.Sheila HT, (ASCP)DPPKnoxville, TN
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