[Histonet] MSH2 and MSH6 validation

[Jacquilyn Vergara via Histonet]

We are having the same issue.
I came across this thread.
Did you find any tissue for this?
Thanks!


Jacquilyn R. Vergara MLS, HTL (ASCP)cm
Email: jverg...@micropathlabs.com
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Re: [Histonet] [External Sender] Re: Closed doors in Histology

[Hannen, Valerie via Histonet]

We have kept our doors closed at all times for years due to the negative 
pressure issue.


Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com




-Original Message-
From: Morken, Timothy via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, November 16, 2018 11:59 AM
To: Histonet
Subject: [External Sender] Re: [Histonet] Closed doors in Histology

WARNING: This message came from an external source. Please do not click links 
or open attachments if unexpected or unusual.

That'll be tough for our histo lab - there are no doors. It is open to two 
hallways and adjacent clin lab. That said, we have so many hoods in there that 
we probably make the entire floor negative pressure! And there is no odor from 
the lab.

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center


-Original Message-
From: Mike Pence via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, November 16, 2018 8:23 AM
To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: [Histonet] Closed doors in Histology

OK, How many of you keep your doors to your Histology Dept. closed all the 
time? We were told by a CIHQ inspector that Histology Dept must be under 
negative air flow ALL THE TIME. This was a new one for me. The standard is from 
the ASHRAE 170 table 7.1
This is just an FYI for you all that these are the kinds of things CMS is 
looking at now days.

Michael S. Pence | Supervisor of Laboratory Services
Great River Health Systems
1221 S. Gear Ave. | West Burlington, IA 52655
Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557
mpe...@grhs.net | 
www.greatrivermedical.org.
www.Facebook.com/GreatRiverHealthSystems
 | www.Twitter/GreatRiverMed


Information in this communication, including attachments, is confidential and 
intended only for the addressee(s). This communication may contain privileged, 
confidential, proprietary or trade secret information entitled to protection or 
exemption from disclosure under law. If you are not an intended recipient, 
please know that any use, distribution or copying of this communication, or any 
action taken based on the information in this communication, is unauthorized 
and may be unlawful. If you received this communication in error, please notify 
the sender and delete this communication from your device.



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[Histonet] Artifact

[Campbell, Tasha M. via Histonet]

Can anyone help me out with the artifact I am getting on my slides?  It is on 
all of them, IHC, H&E, specials.  It looks like formalin pigment, but I use 
10%NBF so should I really be getting formalin pigment this much on my slides?  
I am a GI lab. I use microwave processing. Specimens are held 24-48 hours in 
formalin before being processed. It is on almost all my slides. Everyday.  Its 
very frustrating trying to figure out the problem.  If it is formalin pigment, 
how can I prevent it?  My microwave process is a rinse in 95% alcohol, then 
100% reagent alcohol for 8 min, Isopropyl for 9 minutes, and Paraffin for 14 
min.  I also use a slide etcher to label my slides and there are always dust 
particles in my water bath and getting on my slides.  Could this be causing an 
issue?

I tried to add a screenshot of the slides with the pigment but apparently 
adding anything like that to the message will not let the email go through.  So 
I can privately email the images if someone could help me.  Thanks!



Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 144


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Re: [Histonet] Closed doors in Histology

[Morken, Timothy via Histonet]

That'll be tough for our histo lab - there are no doors. It is open to two 
hallways and adjacent clin lab. That said, we have so many hoods in there that 
we probably make the entire floor negative pressure! And there is no odor from 
the lab.

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center


-Original Message-
From: Mike Pence via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, November 16, 2018 8:23 AM
To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: [Histonet] Closed doors in Histology

OK, How many of you keep your doors to your Histology Dept. closed all the 
time? We were told by a CIHQ inspector that Histology Dept must be under 
negative air flow ALL THE TIME. This was a new one for me. The standard is from 
the ASHRAE 170 table 7.1
This is just an FYI for you all that these are the kinds of things CMS is 
looking at now days.

Michael S. Pence | Supervisor of Laboratory Services
Great River Health Systems
1221 S. Gear Ave. | West Burlington, IA 52655
Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557
mpe...@grhs.net | 
www.greatrivermedical.org.
www.Facebook.com/GreatRiverHealthSystems
 | www.Twitter/GreatRiverMed


Information in this communication, including attachments, is confidential and 
intended only for the addressee(s). This communication may contain privileged, 
confidential, proprietary or trade secret information entitled to protection or 
exemption from disclosure under law. If you are not an intended recipient, 
please know that any use, distribution or copying of this communication, or any 
action taken based on the information in this communication, is unauthorized 
and may be unlawful. If you received this communication in error, please notify 
the sender and delete this communication from your device.



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Re: [Histonet] Closed doors in Histology

[Victoria Baker via Histonet]

Mike,

Yes that is correct.  Histology labs because of their toxic chemicals and
less than pleasant odors need to keep the doors shut for proper
ventilation.  This doesn't always happen when there is a high volume of
people going in and out.
I like it to the requirement that there be a sink (clean sink) in every
Histology lab too.  We have two sinks, 1 for special stains and the other
for tissue processor loading and formalin neutralization.  Which sink do
you think might be cleaner

Vikki Baker

On Fri, Nov 16, 2018, 11:35 AM Mike Pence via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> OK, How many of you keep your doors to your Histology Dept. closed all the
> time? We were told by a CIHQ inspector that Histology Dept must be under
> negative air flow ALL THE TIME. This was a new one for me. The standard is
> from the ASHRAE 170 table 7.1
> This is just an FYI for you all that these are the kinds of things CMS is
> looking at now days.
>
> Michael S. Pence | Supervisor of Laboratory Services
> Great River Health Systems
> 1221 S. Gear Ave. | West Burlington, IA 52655
> Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557
> mpe...@grhs.net | www.greatrivermedical.org<
> http://www.greatrivermedical.org>.
> www.Facebook.com/GreatRiverHealthSystems<
> http://www.Facebook.com/GreatRiverHealthSystems> |
> www.Twitter/GreatRiverMed
>
>
> Information in this communication, including attachments, is confidential
> and intended only for the addressee(s). This communication may contain
> privileged, confidential, proprietary or trade secret information entitled
> to protection or exemption from disclosure under law. If you are not an
> intended recipient, please know that any use, distribution or copying of
> this communication, or any action taken based on the information in this
> communication, is unauthorized and may be unlawful. If you received this
> communication in error, please notify the sender and delete this
> communication from your device.
>
>
>
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Re: [Histonet] test

[Rene J Buesa via Histonet]

If you add anything, and I mean ANITHING, to your message, the message will not 
be posted.I recommend you to post your problem again without the 
screenshot.René 

On Friday, November 16, 2018 11:27 AM, "Campbell, Tasha M. via Histonet" 
 wrote:
 

 Thanks.  So I got a copy of this email I sent.  But I sent my question twice 
and did not get the copy.  I put a screen shot in the message.  Could that mess 
it up?

 
 
 
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 144 
-Original Message-
From: Eileen Akemi Allison [mailto:akemiat3...@gmail.com] 
Sent: Friday, November 16, 2018 11:01 AM
To: Campbell, Tasha M.; Histonet
Subject: Re: [Histonet] test

** CAUTION: This email originated from outside of Frederick Memorial Hospital. 
DO NOT click on links or open attachments if you do not recognize the sender. **


I received it.  I have had the same problem lately.  I didn't get a copy of the 
email sent.  It would be nice to know that it was received. Think they changed 
their format.

Akemi Allison

> On Nov 16, 2018, at 7:25 AM, Campbell, Tasha M. via Histonet 
>  wrote:
>
> I keep trying to send an email for help with an issue I am having and its not 
> going through. It says the email has been sent but I am not getting the email 
> and no one has responded.  I am testing to see if this message goes through.
>
>
>
>
> Tasha Campbell, B.S.,HTL(ASCP)
> Frederick Gastroenterology Associates
> 310 W. 9th St.
> Frederick, MD 21701
> 301-695-6800 ext. 144
>
>
> CONFIDENTIALITY NOTICE: This electronic mail transmission and any 
> accompanying data files is confidential and is intended exclusively for the 
> individual or entity to which it is addressed. The communication may contain 
> information that is proprietary, privileged or confidential or otherwise 
> legally exempt from disclosure. If you are not the named addressee or you 
> otherwise have received this message in error, you are not authorized to 
> read, print, copy or disseminate this message or any part of it. If you have 
> received this message in error, please notify the sender immediately by email 
> and delete all copies of this message. Receipt by anyone other than the named 
> addressee is not a waiver of any attorney-client work product or other 
> applicable privilege.
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[Histonet] Reproducibility in IHC validations

[Martha Ward-Pathology via Histonet]

My medical director and I are working on our procedure for reproducibility in 
our IHC validations and would like to know how others are handling this. 
How many slides do you run and over how many days/runs?If someone would 
share their procedure with us that would be wonderful.
Thanks in advance for your help.
Martha Ward
Wake Forest Baptist Health
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[Histonet] Closed doors in Histology

[Mike Pence via Histonet]

OK, How many of you keep your doors to your Histology Dept. closed all the 
time? We were told by a CIHQ inspector that Histology Dept must be under 
negative air flow ALL THE TIME. This was a new one for me. The standard is from 
the ASHRAE 170 table 7.1
This is just an FYI for you all that these are the kinds of things CMS is 
looking at now days.

Michael S. Pence | Supervisor of Laboratory Services
Great River Health Systems
1221 S. Gear Ave. | West Burlington, IA 52655
Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557
mpe...@grhs.net | 
www.greatrivermedical.org.
www.Facebook.com/GreatRiverHealthSystems
 | www.Twitter/GreatRiverMed


Information in this communication, including attachments, is confidential and 
intended only for the addressee(s). This communication may contain privileged, 
confidential, proprietary or trade secret information entitled to protection or 
exemption from disclosure under law. If you are not an intended recipient, 
please know that any use, distribution or copying of this communication, or any 
action taken based on the information in this communication, is unauthorized 
and may be unlawful. If you received this communication in error, please notify 
the sender and delete this communication from your device.



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Re: [Histonet] test

[Eileen Akemi Allison via Histonet]

I received it.  I have had the same problem lately.  I didn’t get a copy of the 
email sent.  It would be nice to know that it was received. Think they changed 
their format.

Akemi Allison

> On Nov 16, 2018, at 7:25 AM, Campbell, Tasha M. via Histonet 
>  wrote:
> 
> I keep trying to send an email for help with an issue I am having and its not 
> going through. It says the email has been sent but I am not getting the email 
> and no one has responded.  I am testing to see if this message goes through.
> 
> 
> 
> 
> Tasha Campbell, B.S.,HTL(ASCP)
> Frederick Gastroenterology Associates
> 310 W. 9th St.
> Frederick, MD 21701
> 301-695-6800 ext. 144
> 
> 
> CONFIDENTIALITY NOTICE: This electronic mail transmission and any 
> accompanying data files is confidential and is intended exclusively for the 
> individual or entity to which it is addressed. The communication may contain 
> information that is proprietary, privileged or confidential or otherwise 
> legally exempt from disclosure. If you are not the named addressee or you 
> otherwise have received this message in error, you are not authorized to 
> read, print, copy or disseminate this message or any part of it. If you have 
> received this message in error, please notify the sender immediately by email 
> and delete all copies of this message. Receipt by anyone other than the named 
> addressee is not a waiver of any attorney-client work product or other 
> applicable privilege.
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Re: [Histonet] test

[Campbell, Tasha M. via Histonet]

Thanks.  So I got a copy of this email I sent.  But I sent my question twice 
and did not get the copy.  I put a screen shot in the message.  Could that mess 
it up?

 
 
 
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 144 
-Original Message-
From: Eileen Akemi Allison [mailto:akemiat3...@gmail.com] 
Sent: Friday, November 16, 2018 11:01 AM
To: Campbell, Tasha M.; Histonet
Subject: Re: [Histonet] test

** CAUTION: This email originated from outside of Frederick Memorial Hospital. 
DO NOT click on links or open attachments if you do not recognize the sender. **


I received it.  I have had the same problem lately.  I didn't get a copy of the 
email sent.  It would be nice to know that it was received. Think they changed 
their format.

Akemi Allison

> On Nov 16, 2018, at 7:25 AM, Campbell, Tasha M. via Histonet 
>  wrote:
>
> I keep trying to send an email for help with an issue I am having and its not 
> going through. It says the email has been sent but I am not getting the email 
> and no one has responded.  I am testing to see if this message goes through.
>
>
>
>
> Tasha Campbell, B.S.,HTL(ASCP)
> Frederick Gastroenterology Associates
> 310 W. 9th St.
> Frederick, MD 21701
> 301-695-6800 ext. 144
>
>
> CONFIDENTIALITY NOTICE: This electronic mail transmission and any 
> accompanying data files is confidential and is intended exclusively for the 
> individual or entity to which it is addressed. The communication may contain 
> information that is proprietary, privileged or confidential or otherwise 
> legally exempt from disclosure. If you are not the named addressee or you 
> otherwise have received this message in error, you are not authorized to 
> read, print, copy or disseminate this message or any part of it. If you have 
> received this message in error, please notify the sender immediately by email 
> and delete all copies of this message. Receipt by anyone other than the named 
> addressee is not a waiver of any attorney-client work product or other 
> applicable privilege.
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[Histonet] test

[Campbell, Tasha M. via Histonet]

I keep trying to send an email for help with an issue I am having and its not 
going through. It says the email has been sent but I am not getting the email 
and no one has responded.  I am testing to see if this message goes through.




Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 144


CONFIDENTIALITY NOTICE: This electronic mail transmission and any accompanying 
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