[Histonet] IHC control blocks, DAB kit

[Cassie P. Davis via Histonet]

Kristy, this might be of some help too: 
https://www.fixationonhistology.com/post/control-tissue-faqs
[https://static.wixstatic.com/media/128854_2fda1d8f8d5c48cdad7701b3e866d885~mv2.jpg/v1/fit/w_1000,h_728,al_c,q_80/file.png]
Control Tissue FAQs - 
fixationonhistology.com
Should control slides be refrigerated? Some controls such as placenta, p53, and 
p57, for example, may lose antigenicity (the antigen’s ability to bind with the 
products of the cell mediated response, like B-cells and T-cells), if they are 
left out. Others are fine being left on the shelf. Whether or not you need to 
refrigerate them also depends on how far in advance they have been cut.
www.fixationonhistology.com


Cassie Davis
Histology Department
Anatomical Pathology Laboratory
302-575-8095
Email:  cda...@che-east.org

W  302-575-8095

701 North Clayton St.
Wilmington, DE 19805

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Re: [Histonet] p53

[John Garratt via Histonet]

http://cpqa.ca/assessments/Run%2095%20p53%20Summary%20(final).pdf

The above is an EQA report from CPQA /CIQC for p53 which gives specific 
protocols. In addition, if you go to the end of the report, there are the 
reporting guidelines for p53.
EQA reports are also available for other antibodies when you go to our web site.


John


www.cpqa.ca

‐‐‐ Original Message ‐‐‐
On Monday, May 4, 2020 7:40 AM, Jacque Sagasser via Histonet 
 wrote:

> Hello,
>
> Our lab has been using this down time to validate a few antibodies. So far we 
> have validated Ki67, AE 1/3, CK20, and CDX-2. I am in the process of 
> validating the P53 (DO-7) antibody, but am having trouble producing positive 
> expression. I am using the same colon adenocarcinoma tissue I used to 
> validate all of the antibodies that I previously mentioned. We are using the 
> Ventana Benchmark XT along with the OptiView DAB. The package insert says to 
> use colon adenocarcinoma for a control tissue, and the iView DAB. In the 
> past, I have worked at a facility that ran it using the UltraView DAB so I 
> don't think using the OptiView DAB is the issue. My Roche representative said 
> he has heard of customers having to use a different control for the P53. Do 
> you have any suggestions for controls or tweaking the protocol for this 
> antibody given the parameters we are using? Any advice would be appreciated.
>
> Jacque R. Sagasser, HT (ASCP)cm
> Gandhi GI Pathology, LLC
> Kettering OH
>
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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[Histonet] p53

[Jacque Sagasser via Histonet]

Hello,

Our lab has been using this down time to validate a few antibodies. So far we 
have validated Ki67, AE 1/3, CK20, and CDX-2. I am in the process of validating 
the P53 (DO-7) antibody, but am having trouble producing positive expression. I 
am using the same colon adenocarcinoma tissue I used to validate all of the 
antibodies that I previously mentioned. We are using the Ventana Benchmark XT 
along with the OptiView DAB. The package insert says to use colon 
adenocarcinoma for a control tissue, and the iView DAB. In the past, I have 
worked at a facility that ran it using the UltraView DAB so I don't think using 
the OptiView DAB is the issue. My Roche representative said he has heard of 
customers having to use a different control for the P53. Do you have any 
suggestions for controls or tweaking the protocol for this antibody given the 
parameters we are using?  Any advice would be appreciated.

​Jacque R. Sagasser, HT (ASCP)cm
Gandhi GI Pathology, LLC
Kettering OH

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