Re: [Histonet] Active Caspase-3 IHC-P staining

[Val Tolley via Histonet]

If you have access to tissues, rather than purchasing cell lines, you can use 
spleen, gut, lymph nodes.  They will all have a lot of apoptosis. You can use a 
lot of different tissues for negative controls: I prefer brain or muscle since 
they are less dense with nuclei.

Val

> On Jun 23, 2022, at 10:32 AM, Charles Riley via Histonet 
>  wrote:
> 
> I am currently running a research project and the researcher wants me to
> use Caspase-3 for apoptosis staining. I have never worked with this
> antibody before and want to know if anyone has any suggestions on what to
> use for a positive tissue control and a negative tissue control?
> 
> 
> I purchased the antibody from Novus Biologicals and they recommend a Jurkat
> cell lysate for positive control but I am not familiar with this as well.
> 
> Any guidance would be greatly appreciated
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Re: [Histonet] Active Caspase-3 IHC-P staining

[Porter, Amy via Histonet]

What species are you staining??

Amy S. Porter, HT (ASCP) - Lab Supervisor
Michigan State University
Departments of Physiology & Pathology
Investigative HistoPathology Lab
567 Wilson Road - Rm 2133
East Lansing, MI  48824
Ph:  517-884-5026
(she/her/hers)
https://sites.google.com/msu.edu/ihpl/home
"Life is like riding a bicycle. To keep your balance, you must keep moving." - 
Albert Einstein


-Original Message-
From: Charles Riley via Histonet  
Sent: Thursday, June 23, 2022 1:19 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Active Caspase-3 IHC-P staining

I am currently running a research project and the researcher wants me to use 
Caspase-3 for apoptosis staining. I have never worked with this antibody before 
and want to know if anyone has any suggestions on what to use for a positive 
tissue control and a negative tissue control?


I purchased the antibody from Novus Biologicals and they recommend a Jurkat 
cell lysate for positive control but I am not familiar with this as well.

Any guidance would be greatly appreciated 
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[Histonet] Active Caspase-3 IHC-P staining

[Charles Riley via Histonet]

I am currently running a research project and the researcher wants me to
use Caspase-3 for apoptosis staining. I have never worked with this
antibody before and want to know if anyone has any suggestions on what to
use for a positive tissue control and a negative tissue control?


I purchased the antibody from Novus Biologicals and they recommend a Jurkat
cell lysate for positive control but I am not familiar with this as well.

Any guidance would be greatly appreciated
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[Histonet] Tips for Self Care because #ilovemyhistopeeps And some fantastic NEW Job opportunities to share!

[relia1--- via Histonet]

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