Re: [Histonet] Formalin pH

2023-09-13 Thread Tony Henwood via Histonet 
Hew-Shue (1991) has described a useful pH indicator for working neutral 
buffered formalin solutions. Bromocresol purple, when added to formalin 
solutions, serves as an indicator of pH as well as, from a safety aspect, 
labelling the solution as formalin making redundant the dangerous “smell” test. 
At an acidic pH (5.2) the intense indicator colour is yellow whereas at pH 6.8, 
the colour is purple. A saturated solution of the dye is prepared, and 2 to 4 
drops are added to 10 litres of neutral buffered formalin. Other advantages are 
that the fixative is more readily distinguished from other colourless solutions 
such as saline, thus preventing accidental misuse, and formalin spills are more 
easily recognised.

Hew-Shue N (1991) “Bromcresol Purple as a Colored Marker and pH Indicator for 
Ten Percent Neutral Buffered Forrnalin” J Histotechnol 14(4):257-260.


Regards,

Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired)
Principal Scientist, the Children’s Hospital at Westmead (Retired)
Adjunct Fellow, School of Medicine, University of Western Sydney.

From: Bacon, Charles via Histonet
Sent: Thursday, 14 September 2023 6:42 AM
To: e...@pigs.ag
Cc: HistoNet
Subject: Re: [Histonet] Formalin pH

We pull the COA from the vendors website. Some are better than others but the 
are usually listed by lot number. I keep them in a network shared folder so 
they can be pulled by anyone that is asked for the evidence during inspection.

Sent from my iPhone

> On Sep 13, 2023, at 8:22 AM, "e...@pigs.ag"  wrote:
>
> Sooner or later the people have got to rise up
> and tell those brain-dead paper-shuffling regulators
> to just stuff it.  Their mindless interference is
> just wasting time and interfering with people's
> ability to keep their own garden.
>
> -Original Message-
> From: Cooper, Brian via Histonet
> Reply-To: Cooper, Brian
> To: Paula Sicurello, HistoNet
> Subject: Re: [Histonet] Formalin pH
> Date: Today 10:19 AM
>
> Ask your vendor to send a certificate of analysis that includes the pH. 
> That's what we did and now they come with each lot. We do have to bug them 
> for them occasionally.
>
> I'm betting your vendor has at least heard about this from other customers so 
> it should be on their radar at the very least.
>
>
> Thanks,
>
>
>
> Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor
>
> Department of Pathology and Laboratory Medicine
>
> Children's Hospital Los Angeles
>
> 4650 Sunset Blvd MS#43- Los Angeles, CA 90027
>
> Ph: 323.361.3357
>
> bcoo...@chla.usc.edu
>
> 
> From: Paula Sicurello via Histonet 
> Sent: Tuesday, September 12, 2023 7:12:05 PM
> To: HistoNet 
> Subject: [Histonet] Formalin pH (EXTERNAL EMAIL)
>
> CAUTION: BE CAREFUL WITH THIS MESSAGE*
> This email came from outside CHLA. Do not open attachments, click on links, 
> or respond unless you expected this message and recognize the email address: 
> histonet-boun...@lists.utsouthwestern.edu.
>
> Hello Histoteckies,
> What are y'all doing regarding the CAP requirement to monitor the pH of 
> formalin?
> We buy tons and tons of the 5 gallon cubitainers and we are still debating 
> over how to check the pH.
>
> Looking forward to your replies.
> Toodles!
> Sincerely,
>
> Paula Sicurello
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> https://urldefense.com/v3/__http://secure-web.cisco.com/1dY8pbLOSpzQKfWx-yvsO5rujytRiLi5FacpaIq-afFO3eWHoj801ibzodIvkn6kWJvH0G2K0mQEZsm-bweaI-0WiwrKEp9XDD-CIrZCerqewH6wfWut7f2aExx7-0Qp1t67zvWQEZovO1QSD1rx0Pyyf0mc80Xc9xXtgj0R1PDbB8vEDPEepcTWafNdNrZKsWKkUwZlF-2tvTQzsn9RxxaQkzzP3rKjsm9Sd35YA9q2N3LM1LbKMIK3GMBAEFn-4HOieXd2nYDQiB3I8SNmwcRDaEoCsyfVtXFrBlJaPmty9xCB6U_ao9WhiJsiDs0mKiphCHZl4SRW8cWwawwDQfNLVDcfj-ajpmEc1E_8mhQwbpcLwmjVDpeOEm7JKweDGFRg3cAe7cz-_6LgHJAxTkvDl6B6uUzM9ak7s-E1aIYOLRgnPn61BtAnF5gQ6ncGtrYsdaqVIpAjc_3ZQEHXIGQ/http*3A*2F*2Flists.utsouthwestern.edu*2Fmailman*2Flistinfo*2Fhistonet__;JSUlJSUl!!P_vj-BUkwjF5!YyUGwYyrMdYlhUlXnDMq68DTxOX1KGg3ZgSeT5GCab42ZrSt48hyrjyqzddx8zyiZ1dZlpGtq26xTL_0DeXre8ERyLwEfuZBEZQRRePe6Pt9wgc$

Re: [Histonet] Formalin pH

2023-09-13 Thread Bacon, Charles via Histonet 
We pull the COA from the vendors website. Some are better than others but the 
are usually listed by lot number. I keep them in a network shared folder so 
they can be pulled by anyone that is asked for the evidence during inspection. 

Sent from my iPhone

> On Sep 13, 2023, at 8:22 AM, "e...@pigs.ag"  wrote:
> 
> Sooner or later the people have got to rise up 
> and tell those brain-dead paper-shuffling regulators
> to just stuff it.  Their mindless interference is 
> just wasting time and interfering with people's
> ability to keep their own garden.
> 
> -Original Message-
> From: Cooper, Brian via Histonet
> Reply-To: Cooper, Brian
> To: Paula Sicurello, HistoNet
> Subject: Re: [Histonet] Formalin pH
> Date: Today 10:19 AM
> 
> Ask your vendor to send a certificate of analysis that includes the pH. 
> That's what we did and now they come with each lot. We do have to bug them 
> for them occasionally.
> 
> I'm betting your vendor has at least heard about this from other customers so 
> it should be on their radar at the very least.
> 
> 
> Thanks,
> 
> 
> 
> Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor
> 
> Department of Pathology and Laboratory Medicine
> 
> Children's Hospital Los Angeles
> 
> 4650 Sunset Blvd MS#43- Los Angeles, CA 90027
> 
> Ph: 323.361.3357
> 
> bcoo...@chla.usc.edu
> 
> 
> From: Paula Sicurello via Histonet 
> Sent: Tuesday, September 12, 2023 7:12:05 PM
> To: HistoNet 
> Subject: [Histonet] Formalin pH (EXTERNAL EMAIL)
> 
> CAUTION: BE CAREFUL WITH THIS MESSAGE*
> This email came from outside CHLA. Do not open attachments, click on links, 
> or respond unless you expected this message and recognize the email address: 
> histonet-boun...@lists.utsouthwestern.edu.
> 
> Hello Histoteckies,
> What are y'all doing regarding the CAP requirement to monitor the pH of 
> formalin?
> We buy tons and tons of the 5 gallon cubitainers and we are still debating 
> over how to check the pH.
> 
> Looking forward to your replies.
> Toodles!
> Sincerely,
> 
> Paula Sicurello
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> https://urldefense.com/v3/__http://secure-web.cisco.com/1dY8pbLOSpzQKfWx-yvsO5rujytRiLi5FacpaIq-afFO3eWHoj801ibzodIvkn6kWJvH0G2K0mQEZsm-bweaI-0WiwrKEp9XDD-CIrZCerqewH6wfWut7f2aExx7-0Qp1t67zvWQEZovO1QSD1rx0Pyyf0mc80Xc9xXtgj0R1PDbB8vEDPEepcTWafNdNrZKsWKkUwZlF-2tvTQzsn9RxxaQkzzP3rKjsm9Sd35YA9q2N3LM1LbKMIK3GMBAEFn-4HOieXd2nYDQiB3I8SNmwcRDaEoCsyfVtXFrBlJaPmty9xCB6U_ao9WhiJsiDs0mKiphCHZl4SRW8cWwawwDQfNLVDcfj-ajpmEc1E_8mhQwbpcLwmjVDpeOEm7JKweDGFRg3cAe7cz-_6LgHJAxTkvDl6B6uUzM9ak7s-E1aIYOLRgnPn61BtAnF5gQ6ncGtrYsdaqVIpAjc_3ZQEHXIGQ/http*3A*2F*2Flists.utsouthwestern.edu*2Fmailman*2Flistinfo*2Fhistonet__;JSUlJSUl!!P_vj-BUkwjF5!YyUGwYyrMdYlhUlXnDMq68DTxOX1KGg3ZgSeT5GCab42ZrSt48hyrjyqzddx8zyiZ1dZlpGtq26xTL_0DeXre8ERyLwEfuZBEZQRRePe6Pt9wgc$
>  
> 
> 
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[Histonet] IHC/Special staining

2023-09-13 Thread Ken M via Histonet 
Does anyone know of any lab with excess capacity good quality and fast 
turnaround that could do IHC/special staining on the side? We are out of 
capacity, and we don't always have the antibodies that we need.

Kevin
kdea...@hotmail.com
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[Histonet] Fw: Reminder Anatomic Pathology Rounds today Wednesday 9/13

2023-09-13 Thread O'Neil, Beth via Histonet 
From: Smittle, Anne 
Sent: Wednesday, September 13, 2023 7:59 AM
Subject: FW: Reminder Anatomic Pathology Rounds today Wednesday 9/13

Good morning,

MS2 PALM Rounds today beginning around 1:00ish today in the Gross Room, 
continuing on into Histology Labs and finishing up in the Residents Library 
looking at current cases with Dr. Williams. Thank you for your assistance with 
the med school students ---next week two Rounds on Tuesday and Wednesday,

Anne Smittle
PALM Course Coordinator
West Virginia University
2190 HSCN
Morgantown, WV 26505
(304) 293-3779
asmit...@hsc.wvu.edu




-Original Message-
From: asmit...@hsc.wvu.edu 
Sent: Wednesday, September 13, 2023 7:57 AM
To: Smittle, Anne 
Subject: Reminder Anatomic Pathology Rounds today 9/13

Reminder AP Rounds today. Check instructions on the Sign Up List for AP Rounds.



Confidentiality Notice: This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential and 
privileged information. Any unauthorized review, use, disclosure or 
distribution is prohibited. If you are not the intended recipient, please 
contact the sender by reply e-mail and destroy all copies of the original 
message.
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