Re: [Histonet] Modified Davidson

[John Kiernan via Histonet]

The "Davidson's" name is, as Tony says, applied to various mixtures. In these, 
formalin is acidified with acetic acid in solutions that also contain some 
alcohol (typically about 33% v/v) but probably not enough to contribute to 
fixation. The mixture quoted from Latendresse et al. 2002 has only 15% alcohol 
- about the same as in a good dry sherry.  (My gastric mucosa is not yet fixed.)

The Histonet archives must surely still contain Dr Bob Richmond's contributions 
about "Davidson's fixative" in the 1990s or 2000s.

Enough from me for now!
John Kiernan
London, Canada
= = =

From: Tony Henwood via Histonet 
Sent: February 8, 2024 5:31 PM
To: Naira Margaryan ; Histonet 

Subject: Re: [Histonet] Modified Davidson

There are several formulations (some are probably typos), but this seems to be 
one commonly cited.

Modified Davidson's Fixative:

(Latendresse, J. R., Warbrittion, A. R., Jonassen, H., & Creasy, D. M. (2002). 
Fixation of testes and eyes using a modified Davidson's fluid: comparison with 
Bouin's fluid and conventional Davidson's fluid. Toxicologic pathology, 30(4), 
524-533.)

37–40% formaldehyde30ml
Absolute ethanol 15ml
Glacial acetic acid5ml
Distilled Water 50ml

Regards,

Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired)
Principal Scientist, the Children’s Hospital at Westmead (Retired)
Adjunct Fellow, School of Medicine, University of Western Sydney.


From: Naira Margaryan via Histonet 
Sent: Friday, February 9, 2024 8:25:47 AM
To: Histonet 
Subject: [Histonet] Modified Davidson

Hello,

Could you please share your best recipe for the Modified Davidson?

Thanks in advance,
Naira
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Modified Davidson

[Tony Henwood via Histonet]

There are several formulations (some are probably typos), but this seems to be 
one commonly cited.

Modified Davidson's Fixative:

(Latendresse, J. R., Warbrittion, A. R., Jonassen, H., & Creasy, D. M. (2002). 
Fixation of testes and eyes using a modified Davidson's fluid: comparison with 
Bouin's fluid and conventional Davidson's fluid. Toxicologic pathology, 30(4), 
524-533.)

37–40% formaldehyde30ml
Absolute ethanol 15ml
Glacial acetic acid5ml
Distilled Water 50ml

Regards,

Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired)
Principal Scientist, the Children’s Hospital at Westmead (Retired)
Adjunct Fellow, School of Medicine, University of Western Sydney.


From: Naira Margaryan via Histonet 
Sent: Friday, February 9, 2024 8:25:47 AM
To: Histonet 
Subject: [Histonet] Modified Davidson

Hello,

Could you please share your best recipe for the Modified Davidson?

Thanks in advance,
Naira
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Modified Davidson

[Naira Margaryan via Histonet]

Hello,

Could you please share your best recipe for the Modified Davidson?

Thanks in advance,
Naira
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Histonet Digest, Vol 243, Issue 6

[Paula Keene Pierce via Histonet]

When I need to handwrite on slides or cassettes, I use Sakura Pigma Micron 
archival ink pens.
They come in different tip sizes and colors and are found at any office supply 
store.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Thursday, February 8, 2024 at 01:43:43 PM CST, KATHLEEN FERNANDEZ via 
Histonet  wrote:  
 
 Paula,
We are using the KP’s at our lab too and have been noticing that they are 
smearing! We are having issues on our slides, since we hand write still. This 
just recently started happening. Maybe they changed their formula?

Kathy
Sent from my iPhone

> On Feb 8, 2024, at 12:01 PM, histonet-requ...@lists.utsouthwestern.edu wrote:
> 
> Send Histonet mailing list submissions to
>    histonet@lists.utsouthwestern.edu
> 
> To subscribe or unsubscribe via the World Wide Web, visit
>    http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
>    histonet-requ...@lists.utsouthwestern.edu
> 
> You can reach the person managing the list at
>    histonet-ow...@lists.utsouthwestern.edu
> 
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> 
> 
> Today's Topics:
> 
>  1. Marking Pen (Paula)
>  2. Re: Marking Pen (Piche, Jessica)
>  3. Lead Histotechnologist (Stephanie L. Thompson)
> 
> 
> --
> 
> Message: 1
> Date: Wed, 7 Feb 2024 11:35:26 -0800
> From: "Paula" 
> To: 
> Subject: [Histonet] Marking Pen
> Message-ID: <009e01da59fc$ce13b680$6a3b2380$@biopath.org>
> Content-Type: text/plain;    charset="us-ascii"
> 
> Hello,
> 
> 
> 
> We have been using KP Marker Plus pens for cassettes.  We have 2 Leica
> Processors and 1 VIP6 processor.  The cassettes that go into the VIP6 are
> smeared and some are almost smeared off completely.  The processors have the
> same solutions in them.
> 
> 
> 
> If anyone can shed some light as to why there is a difference, and if anyone
> can recommend a better marking pen for us to try, I would appreciate the
> feedback.
> 
> 
> 
> Thank you,
> 
> Paula
> 
> 
> 
> --
> 
> Message: 2
> Date: Thu, 8 Feb 2024 11:30:03 +
> From: "Piche, Jessica" 
> To: "histonet@lists.utsouthwestern.edu"
>    ,    Paula 
> Subject: Re: [Histonet] Marking Pen
> Message-ID:
>    
>
>    
> Content-Type: text/plain; charset="us-ascii"
> 
> Hi Paula,
> 
> What is the first solution the cassettes go in when the processor starts? We 
> use Statmark pens when our cassette printer isn't working, and they work 
> well. The only time we have had issues was when we would hand write the 
> cassettes and run them on our small biopsy run which skips the formalin. It 
> seems like the marker ink needs to "fix" with the formalin. Sometimes they 
> smear if they aren't dry enough before they go into formalin too. I hope you 
> figure it out. Maybe see if you can get some samples of different pens and 
> then run some empty cassettes and see what works best for you.
> 
> Have a good day.
> 
> Jessica
> 
> Jessica Piche, HT(ASCP)
> Waterbury Hospital Histology Laboratory
> Histology Team Leader
> 203-573-7167
> 
> From: Paula via Histonet 
> Sent: Wednesday, February 7, 2024 2:35 PM
> To: histonet@lists.utsouthwestern.edu 
> Subject: [Histonet] Marking Pen
> 
> [EXTERNAL MSG]
> 
> Hello,
> 
> 
> 
> We have been using KP Marker Plus pens for cassettes.  We have 2 Leica
> Processors and 1 VIP6 processor.  The cassettes that go into the VIP6 are
> smeared and some are almost smeared off completely.  The processors have the
> same solutions in them.
> 
> 
> 
> If anyone can shed some light as to why there is a difference, and if anyone
> can recommend a better marking pen for us to try, I would appreciate the
> feedback.
> 
> 
> 
> Thank you,
> 
> Paula
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet=05%7C02%7Cjessica.piche%40wtbyhosp.org%7C873b23e6f59c40f09ac508dc2813f9d3%7Cd4e8b650c4e4424481b4a11e2edb35a9%7C0%7C0%7C638429313478635896%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C=eiD1an%2F2n73QFobrsXKdiC2Kk8h6AE78xAtW9DIRiAM%3D=0
> 
> 
> --
> 
> Message: 3
> Date: Thu, 8 Feb 2024 17:19:28 +
> From: "Stephanie L. Thompson" 
> To: "Histonet@lists.utsouthwestern.edu"
>    
> Subject: [Histonet] Lead Histotechnologist
> Message-ID: 
> Content-Type: text/plain; charset="us-ascii"
> 
> Looking for the next step in your career?
> 
> Sonic 

Re: [Histonet] Histonet Digest, Vol 243, Issue 6

[KATHLEEN FERNANDEZ via Histonet]

Paula,
We are using the KP’s at our lab too and have been noticing that they are 
smearing! We are having issues on our slides, since we hand write still. This 
just recently started happening. Maybe they changed their formula?

Kathy
Sent from my iPhone

> On Feb 8, 2024, at 12:01 PM, histonet-requ...@lists.utsouthwestern.edu wrote:
> 
> Send Histonet mailing list submissions to
>histonet@lists.utsouthwestern.edu
> 
> To subscribe or unsubscribe via the World Wide Web, visit
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
>histonet-requ...@lists.utsouthwestern.edu
> 
> You can reach the person managing the list at
>histonet-ow...@lists.utsouthwestern.edu
> 
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> 
> 
> Today's Topics:
> 
>   1. Marking Pen (Paula)
>   2. Re: Marking Pen (Piche, Jessica)
>   3. Lead Histotechnologist (Stephanie L. Thompson)
> 
> 
> --
> 
> Message: 1
> Date: Wed, 7 Feb 2024 11:35:26 -0800
> From: "Paula" 
> To: 
> Subject: [Histonet] Marking Pen
> Message-ID: <009e01da59fc$ce13b680$6a3b2380$@biopath.org>
> Content-Type: text/plain;charset="us-ascii"
> 
> Hello,
> 
> 
> 
> We have been using KP Marker Plus pens for cassettes.  We have 2 Leica
> Processors and 1 VIP6 processor.  The cassettes that go into the VIP6 are
> smeared and some are almost smeared off completely.  The processors have the
> same solutions in them.
> 
> 
> 
> If anyone can shed some light as to why there is a difference, and if anyone
> can recommend a better marking pen for us to try, I would appreciate the
> feedback.
> 
> 
> 
> Thank you,
> 
> Paula
> 
> 
> 
> --
> 
> Message: 2
> Date: Thu, 8 Feb 2024 11:30:03 +
> From: "Piche, Jessica" 
> To: "histonet@lists.utsouthwestern.edu"
>,Paula 
> Subject: Re: [Histonet] Marking Pen
> Message-ID:
>
> 
>
> Content-Type: text/plain; charset="us-ascii"
> 
> Hi Paula,
> 
> What is the first solution the cassettes go in when the processor starts? We 
> use Statmark pens when our cassette printer isn't working, and they work 
> well. The only time we have had issues was when we would hand write the 
> cassettes and run them on our small biopsy run which skips the formalin. It 
> seems like the marker ink needs to "fix" with the formalin. Sometimes they 
> smear if they aren't dry enough before they go into formalin too. I hope you 
> figure it out. Maybe see if you can get some samples of different pens and 
> then run some empty cassettes and see what works best for you.
> 
> Have a good day.
> 
> Jessica
> 
> Jessica Piche, HT(ASCP)
> Waterbury Hospital Histology Laboratory
> Histology Team Leader
> 203-573-7167
> 
> From: Paula via Histonet 
> Sent: Wednesday, February 7, 2024 2:35 PM
> To: histonet@lists.utsouthwestern.edu 
> Subject: [Histonet] Marking Pen
> 
> [EXTERNAL MSG]
> 
> Hello,
> 
> 
> 
> We have been using KP Marker Plus pens for cassettes.  We have 2 Leica
> Processors and 1 VIP6 processor.  The cassettes that go into the VIP6 are
> smeared and some are almost smeared off completely.  The processors have the
> same solutions in them.
> 
> 
> 
> If anyone can shed some light as to why there is a difference, and if anyone
> can recommend a better marking pen for us to try, I would appreciate the
> feedback.
> 
> 
> 
> Thank you,
> 
> Paula
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet=05%7C02%7Cjessica.piche%40wtbyhosp.org%7C873b23e6f59c40f09ac508dc2813f9d3%7Cd4e8b650c4e4424481b4a11e2edb35a9%7C0%7C0%7C638429313478635896%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C=eiD1an%2F2n73QFobrsXKdiC2Kk8h6AE78xAtW9DIRiAM%3D=0
> 
> 
> --
> 
> Message: 3
> Date: Thu, 8 Feb 2024 17:19:28 +
> From: "Stephanie L. Thompson" 
> To: "Histonet@lists.utsouthwestern.edu"
>
> Subject: [Histonet] Lead Histotechnologist
> Message-ID: 
> Content-Type: text/plain; charset="us-ascii"
> 
> Looking for the next step in your career?
> 
> Sonic Healthcare USA has an opportunity for a Lead Histotechnologist.
> 
> Quality is in our DNA -- is it in yours?
> 
> Join our front line of #HealthcareHeroes! Our mission is to advance the 
> health and wellbeing of our communities as a leader in clinical laboratory 
> solutions.
> 
> Location: Exeter, NH
> Days: Monday - Friday
> 9:00 PM - 5:30 AM
> Full-time: Benefit Eligible
> 
> In this role, you will:
> 
> Prepare histologic slides from human tissue sections for microscopic 
> examination and 

[Histonet] Lead Histotechnologist

[Stephanie L. Thompson via Histonet]

Looking for the next step in your career?

Sonic Healthcare USA has an opportunity for a Lead Histotechnologist.

Quality is in our DNA -- is it in yours?

Join our front line of #HealthcareHeroes! Our mission is to advance the health 
and wellbeing of our communities as a leader in clinical laboratory solutions.

Location: Exeter, NH
Days: Monday - Friday
9:00 PM - 5:30 AM
Full-time: Benefit Eligible

In this role, you will:

Prepare histologic slides from human tissue sections for microscopic 
examination and diagnosis by Pathologist.

  *   Exercises independent judgment in dealing with procedural and technical 
problems.
  *   Examines slides and/or blocks to ensure tissue preparation is meeting 
laboratory requirements.
  *   Trains or directs Laboratory Assistants and Histology Technicians engaged 
in laboratory testing and processing techniques.
  *   Prepares sections of human tissue for microscopic examination and patient 
diagnosis, using techniques to gross (dissect tissue), embed (orient specimen 
in paraffin block), section (cut thin sections of tissue), stain (enhance 
contrast of tissue and highlight specific features of interest with routine 
hematoxylin and eosin stains), and mount tissue (adhere tissue onto glass 
slides), from surgical procedures.
  *   Performs recuts and additional stains including special and 
immunohistochemistry stains, as requested by a Pathologist.
  *   Operates computerized laboratory equipment to fix, dehydrate, and 
infiltrate with wax, tissue specimens to be preserved for study by Pathologist.
  *   May label requisitions, specimen containers, cassettes and/or slides and 
affixes coverslip to slides.
  *   Maintains laboratory equipment and tracks all routine maintenance and 
quality controls performed.
  *   Files, retrieves, and distributes blocks, slides, and pathology reports.
  *   Operates, cleans, and sterilizes laboratory equipment, glassware, 
instruments, and workstation.
  *   Disposes of hazardous chemical waste per regulatory guidelines.
  *   Maintains strictest confidentiality.
  *   Complies with all State, Federal, professional regulations as well as 
company and departmental rules, polices, and procedural manuals.
  *   Adherence to CAP, CLIA, State Regulations, HIPAA, Safety and OSHA 
Regulations.

All you need is:

  *   High School diploma or equivalent required. Associates or Bachelors of 
Science degree and completion of histotechnology program required. 
Certification as a histotechnician (HT) or histotechnologist (HTL) by American 
Society of Clinical Pathology (ASCP) preferred.
  *   State licensure, if applicable.
  *   Certified or eligible for Board of Certification (BOC) by the American 
Society of Clinical Pathologists (ASCP)
  *   Completion of a Histology program accredited by the National Accrediting 
Agency for Clinical Laboratory Sciences (NAACLS) or minimum of one (1) year 
experience as a full-time Histology Technician Trainee and competent in the 
areas of fixation, processing, embedding, microtomy, grossing, special stains, 
immunohistochemistry, and lab operations.

Company:
Sonic Anatomic Pathology

Please feel free to contact me:
Stephanie Thompson - 210-428-1646



This message contains privileged and confidential information intended only for 
the use of the addressee named above. If you are not the intended recipient of 
this message you must not disseminate, copy, or take any action in reliance on 
it.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Marking Pen

[Piche, Jessica via Histonet]

Hi Paula,

What is the first solution the cassettes go in when the processor starts? We 
use Statmark pens when our cassette printer isn't working, and they work well. 
The only time we have had issues was when we would hand write the cassettes and 
run them on our small biopsy run which skips the formalin. It seems like the 
marker ink needs to "fix" with the formalin. Sometimes they smear if they 
aren't dry enough before they go into formalin too. I hope you figure it out. 
Maybe see if you can get some samples of different pens and then run some empty 
cassettes and see what works best for you.

Have a good day.

Jessica

Jessica Piche, HT(ASCP)
Waterbury Hospital Histology Laboratory
Histology Team Leader
203-573-7167

From: Paula via Histonet 
Sent: Wednesday, February 7, 2024 2:35 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Marking Pen

[EXTERNAL MSG]

Hello,



We have been using KP Marker Plus pens for cassettes.  We have 2 Leica
Processors and 1 VIP6 processor.  The cassettes that go into the VIP6 are
smeared and some are almost smeared off completely.  The processors have the
same solutions in them.



If anyone can shed some light as to why there is a difference, and if anyone
can recommend a better marking pen for us to try, I would appreciate the
feedback.



Thank you,

Paula

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet=05%7C02%7Cjessica.piche%40wtbyhosp.org%7C873b23e6f59c40f09ac508dc2813f9d3%7Cd4e8b650c4e4424481b4a11e2edb35a9%7C0%7C0%7C638429313478635896%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C=eiD1an%2F2n73QFobrsXKdiC2Kk8h6AE78xAtW9DIRiAM%3D=0
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet