[Histonet] Dry Film Coverslipping?

[Cates, Julia via Histonet]

Hello Histonet,

I have been asked to consider going Xylene free.  The only hang up that I am 
having is the coverslipping part.  We have an old Sakura Film coverslipper and 
I don't know how that would work without xylene.  When I did some searching in 
the archives, I found some comments made by Rene, that he used this type of 
coverslipper on oven dried slides but never referenced how.  Does anyone use 
this method in your lab?  If so, would you share your protocol and are there 
any problems to consider?

Thanks,

Julia C.


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Re: [Histonet] The Use of Plants in Histology Laboratories

[Cates, Julia via Histonet]

I think from a safety and infection control perspective, house plants, while 
beneficial for air quality, cannot be cleaned or disinfected.  An environment 
of care committee or safety officer would veto the plants based on the that. 

Thanks,

Julia Cates, HT(ASCP)cm
Pathology Coordinator, Pathology
Park Ridge Health
(828)650-8243| Fax: (828)209-5315

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-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 
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To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Histonet Digest, Vol 168, Issue 12

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Today's Topics:

   1. Re: Mounting media (Terri  Braud)
   2. 2018 FSH Annual Meeting (John Shelley)
   3. Re: The Use of Plants in Histology Laboratories (Mickley,
  Beth) (Mayer,Toysha N)


--

Message: 1
Date: Tue, 14 Nov 2017 18:34:23 +
From: "Terri  Braud" 
To: "'histonet@lists.utsouthwestern.edu'"

Subject: Re: [Histonet] Mounting media
Message-ID:

<48E053DDF6CE074DB6A7414BA05403F84CE9DE70@HRHEX03-HOS.holyredeemer.local>

Content-Type: text/plain; charset="us-ascii"

Although we've used several brands with good success, our most consistent 
performer has been the Sakura Tissue Tek Glas Mounting Media #6419. When 
coverslipping, either automated or manual, the secret to avoiding air bubble 
during storage is to insure that the correct amount of media is dispensed onto 
the slide.  If the amount is insufficient, the slide will still coverslip to be 
read, but as time passes and the xylene dries out, there will be air left under 
the coverglass which will allow the stain to degrade. When coverslipping by 
hand, we go by the rule of 3 drops of media from a plastic disposable pipette 
for a 24x50 No.1 coverglass.  When techs "guesstimate" is when problems occur.  
Best of luck, I hope this helps

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal





--

Message: 2
Date: Tue, 14 Nov 2017 19:06:26 +
From: John Shelley 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] 2018 FSH Annual Meeting
Message-ID:

Content-Type: text/plain; charset="us-ascii"

Hello Histonetters!

The FSH Annual Meeting will be in Tampa, FL on May 17-20, 2018 at the 
Renaissance Tampa International Plaza Hotel.

We are looking for ideas for classes that you will be interested in attending.

We are also looking for speakers. Send abstracts to email below.

We want to hear from you. Contact us at fshgrouppresidentgmail.com

See you in Tampa.

Kind Regards!

John J Shelley
fshgrouppresid...@gmail.com



--

Message: 3
Date: Tue, 14 Nov 2017 21:10:33 +
From: "Mayer,Toysha N" 
To: "'histonet@lists.utsouthwestern.edu'"

Subject: Re: [Histonet] The Use of Plants in Histology Laboratories
(Mickley,   Beth)
Message-ID:
<47e9b2c0194881eacd2dc44ebc883a065...@d1pwpexmbx08.mdanderson.edu>
Content-Type: text/plain; charset="us-ascii"

Beth,

We sure could have used the actual article in a lab I know of.  The person with 
the highest authority, removed them from a lab, and did not want to listen to 
what the supervisor had to say.  Without the actual article, nothing could 
change her mind.
It is common to have spider plants, and ivy in labs to help with the air 
quality. 
Now the EHS departments need to know about it as well.

Toysha

Message: 1
Date: Mon, 13 Nov 2017 20:24:31 

[Histonet] A moment of thanks

[Cates, Julia via Histonet]

Good Morning Histonet!

I wanted to take a moment to express my gratitude to all of my colleagues on 
histonet. You are an invaluable resource in a world that is spread-out and I 
treasure the ability to reach out to you when I have a problem.

Case and point,  I have been struggling with a H problem for months.  When 
looking at the slide, you would think the problem was a cutting artifact.  I 
had a service technician go over the microtome multiple times with minimal 
resolution.
The second part of the problem is that the issue was intermittent.  Anyone 
troubleshooting knows how confounding this can be!  After exhausting all 
cutting possibilities, I started looking at the stainer. That is where histonet 
came on to the scene.  I searched hoping to find someone who had had the same 
problem or similar and sure enough I found it.  One response from Tim Morken 
has solved my issue and I can't thank you enough.

I will say that this is also an important lesson for me as well.  I should have 
reached out sooner for help.  I wouldn't have had to struggle or put my 
pathologist through looking at garbage slides.  Lesson learned.

Anyway, my thanks again to you all!

Thanks,

Julia Cates, HT(ASCP)cm

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Re: [Histonet] Cold Ischemic time

[Cates, Julia via Histonet]

Hi Cristi,

We have the PA/Pathologist dictate the Ischemic time and total fixation time at 
the end of the gross. It reads something like, "The specimen has an ischemic 
time of 1 hour and is allowed to fix for 48 hours".  As long as it documents 
both times you should be fine.

Thanks,

Julia Cates, HT(ASCP)cm
Pathology Coordinator, Pathology
Park Ridge Health
(828)650-8243| Fax: (828)209-5315

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Re: [Histonet] JC requirement for stain QC question

[Cates, Julia via Histonet]

Hi Tim,

We have a form here that was developed after a similar inspection.  The form 
has quality control indicators for both the histologist and pathologist to 
review.  At the end of the day, the pathologist will initial as reviewed for 
the entire form.  He also documents QC in the patients report for Specials and 
IHC controls.

Thanks,

Julia Cates, HT(ASCP)cm
Pathology Coordinator, Pathology
Park Ridge Health
(828)650-8243| Fax: (828)209-5315

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Re: [Histonet] Formaldehyde annual education

[Cates, Julia via Histonet]

Jim,

You are correct.  OSHA requires annual education for any personnel that handle 
formalin to be educated in the hazards, proper handling, etc... This can be as 
simple or as involved as you want to make it but it should cover the basics at 
the very least.


Thanks,

Julia Cates, HT(ASCP)cm
Pathology Coordinator, Pathology
Park Ridge Health

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Re: [Histonet] GHS vs. NFPA labeling

[Cates, Julia via Histonet]

Oops! I do not believe it is permitted:)  I need to read more carefully before 
I send out emails!!

See the link below for a recent blog entry addressing this very question.  I do 
not believe placing a new label on an original container is not permitted.  I 
would transfer to a secondary container, if able, and label accordingly.

http://danthelabsafetyman.tumblr.com/



Thanks,

Julia Cates, HT(ASCP)cm
Pathology Coordinator, Pathology
Park Ridge Health
(828)650-8243| Fax: (828)209-5315

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Re: [Histonet] GHS vs. NFPA labeling

[Cates, Julia via Histonet]

See the link below for a recent blog entry addressing this very question.  I do 
not believe placing a new label on an original container is not permitted.  I 
would transfer to a secondary container, if able, and label accordingly.

http://danthelabsafetyman.tumblr.com/

Thanks,

Julia Cates, HT(ASCP)cm
Pathology Coordinator, Pathology
Park Ridge Health

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Re: [Histonet] Ventana H.Pylori antibody

[Cates, Julia via Histonet]

Wow!  Thank you all so much for your responses.  They have been very helpful; 
it's so great to have a community of professionals that are so willing to give 
of their knowledge.

Julia Cates, HT(ASCP)cm
Pathology Coordinator, Pathology
Florida Hospital Waterman
(352) 253- ext.4346 | Fax: (352) 253-3592

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Message: 1
Date: Thu, 30 Jul 2015 17:06:04 +
From: Jeffrey Robinson jrobin...@pathology-associates.com
To: Cates, Julia julia.ca...@ahss.org,
histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Ventana H.Pylori antibody
Message-ID:

204A03EB5A7F0A4BB1EEDD52A963829C16D9EB26@PAEXCH1.PathologyAssociates.local

Content-Type: text/plain; charset=us-ascii

Hi Julia-  I have had a lot of experience trying to get a clean H. pylori.  I 
don't recall using the Ventana H. pylori but I was using the CellMarque H. 
pylori for years.  It would often have a lot of background as you describe.  I 
run Ventana Benchmarks (XT and Ultra) and a Leica Bond.  The slides I would run 
on the Benchmarks were usually cleaner than those run on the Bond but still not 
as clean as I would like. I finally switched over to BioCare's H. pylori after 
many complaints from my pathologists.  It has been much cleaner on all of my 
IHC instruments.  Actually, the ones I run on the Ultra are the cleanest I have 
ever seen.  The slides run on the Bond may still have a little background but 
it is still much cleaner than the ones I used to run with the CellMarque 
antibody.  One of my pathologists who used to complain about the excessive 
background now calls it his go to stain and is very happy with how crisp it 
is. The organisms stand out very nicely.  BioCare will send you a free sample 
if you want to try it.
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: Cates, Julia via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, July 30, 2015 8:30 AM
To: histonet-boun...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ventana H.Pylori antibody

Good day Histonet,

I have seen in the past conversations regarding Ventana's H.Pylori antibody and 
how dirty looking the stain can be.  We have been using this antibody for 
several years now and have never really been happy with the quality.  We have 
tried the recommended methods to clean up the stain and still we run into 
repeating the stain and/or complaints from the pathologists.  We are 
considering using a different vendor but my concern is that my efforts will be 
a lateral move.  Is anyone using a product that produces a clean stain or is 
this something inherent to this antibody?  Are the pathologists happy with it 
and not just tolerating it?

Thank you for your suggestions,

Julia Cates, HT(ASCP)cm
Pathology Coordinator, Pathology
Florida Hospital Waterman
(352) 253- ext.4346 | Fax: (352) 253-3592

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for the sole use of the intended recipient and may contain confidential and 
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copies of this email.


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[Histonet] Ventana H.Pylori antibody

[Cates, Julia via Histonet]

Good day Histonet,

I have seen in the past conversations regarding Ventana's H.Pylori antibody and 
how dirty looking the stain can be.  We have been using this antibody for 
several years now and have never really been happy with the quality.  We have 
tried the recommended methods to clean up the stain and still we run into 
repeating the stain and/or complaints from the pathologists.  We are 
considering using a different vendor but my concern is that my efforts will be 
a lateral move.  Is anyone using a product that produces a clean stain or is 
this something inherent to this antibody?  Are the pathologists happy with it 
and not just tolerating it?

Thank you for your suggestions,

Julia Cates, HT(ASCP)cm
Pathology Coordinator, Pathology
Florida Hospital Waterman
(352) 253- ext.4346 | Fax: (352) 253-3592

Confidentiality Statement: This email message, including any attachments, is 
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privileged information. Any unauthorized review, use, disclosure, or 
distribution is prohibited.  If you are not the intended recipient, please 
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copies of this email.


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