Re: [Histonet] IHC on fish
Cate. Please report your findings on this topic. Kind regards. JB On Jun 14, 2015 6:38 AM, Hardy, Cate cha...@csu.edu.au wrote: Hi all; Has anyone performed IHC on fish. I have been asked to perform this using several of our routine antibodies that I use on mammals. My gut tells me it won't work but I am a lowly histotech and don't know anything. Seeking knowledge from learned tech's with experience in such matters Thanks Cate Hardy Senior Technical Officer Veterinary Diagnostic Laboratory Charles Strut University NSW Australia Charles Sturt University | ALBURY-WODONGA | BATHURST | CANBERRA | DUBBO | GOULBURN | MELBOURNE | ONTARIO | ORANGE | PORT MACQUARIE | SYDNEY | WAGGA WAGGA | LEGAL NOTICE This email (and any attachment) is confidential and is intended for the use of the addressee(s) only. If you are not the intended recipient of this email, you must not copy, distribute, take any action in reliance on it or disclose it to anyone. Any confidentiality is not waived or lost by reason of mistaken delivery. Email should be checked for viruses and defects before opening. Charles Sturt University (CSU) does not accept liability for viruses or any consequence which arise as a result of this email transmission. Email communications with CSU may be subject to automated email filtering, which could result in the delay or deletion of a legitimate email before it is read at CSU. The views expressed in this email are not necessarily those of CSU. Charles Sturt University in Australia http://www.csu.edu.au The Grange Chancellery, Panorama Avenue, Bathurst NSW Australia 2795 (ABN: 83 878 708 551; CRICOS Provider Numbers: 5F (NSW), 01947G (VIC), 02960B (ACT)). TEQSA Provider Number: PV12018 Charles Sturt University in Ontario http://www.charlessturt.ca 860 Harrington Court, Burlington Ontario Canada L7N 3N4 Registration: www.peqab.ca Consider the environment before printing this email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Positive Control for IF?
Paula, does your institution perform autopsies? If so, have the staff notify you when there is a lupus nephritis case. Have them send you fresh renal cortex. Cut up the tissue into small cubes and freeze for later use as IF control. Run your battery of Ig's, compliment and light chains. Most should have some reactivity. You might be able to use a rejected kidney for C4d if it isn't to necrotic. Transplant heart and liver can also be used for rejection antibodies. JB On Wednesday, April 1, 2015, Paula Sicurello pat...@gmail.com wrote: Good Afternoon Netters, Since we went down the path of hot dogs and Slim Jim's as positive controls for FFPE stains, I was wondering. Is there a source, like a hot dog or piece of steak, that could be a positive control for immunofluorescence C4d? What I'd really like to find is some food or food product (our positive patient biopsies for the frozen IF are teeny-tiny) that is positive for the whole host of IF stains: IgG, IgA, IgM, Kappa, Lambda, you get the picture. Please send your suggestions my way. Thanks in advance, Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu javascript:; http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jim Burchette Fly Fishing Bum *'(((* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Controls:
So do onions! On Thu, Mar 5, 2015 at 12:06 PM, Cooper, Brian bcoo...@chla.usc.edu wrote: I've used moldy orange peels as a GMS/PAS-F control in the past. Just saved the peels in a plastic bag over the weekend, then processed as usual. They worked beautifully! Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcoo...@chla.usc.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Thursday, March 05, 2015 7:21 AM To: Jb; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls: We have successfully used hamburger meat to make Gram controls. As far as GMS controls go, we ran across a post from someone smearing cream cheese onto lung tissue and letting it sit for a couple of days, fixed and processed it and were able to demonstrate Aspergillus by GMS. My fungus control stocks are low so I was actually planning to try this with some beef lung. I haven't heard of the Slim Jim method before. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Wednesday, March 04, 2015 1:05 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls: Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? Thank you, Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jim Burchette Fly Fishing Bum *'(((* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RENEW YOUR FREE GEORGIA MEMBERSHIP !
The limit for online responses has been met by your service. Jim On Tuesday, January 27, 2015, Zimmerman, Billie bzimm...@gru.edu wrote: http://www.histosearch.com/gsh/ Renew your free Georgia membership and all your histological dreams will come true... Yours truly, Glenda the good witch Secretary GSH ___ Histonet mailing list Histonet@lists.utsouthwestern.edu javascript:; http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jim Burchette Fly Fishing Bum *'(((* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] PML - JC virus
Richard, I always used Oncogenes SV40 antibody for PML. On Dec 29, 2014 3:47 PM, Cartun, Richard richard.car...@hhchealth.org wrote: Can anyone recommend a good antibody (for IHC testing) to identify JC virus (Polyomavirus) in the brain (PML)? I finally ran out of my polyclonal anti-SV40 antibody that I have been using for the past 25 years. Best wishes to everyone for the coming New Year! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Decolorizing effect on IHC stain
I suggest you run two positive controls. One control stained with HE the decolorized. The other as usual. JB On Dec 2, 2014 3:35 AM, Laurie Best lb...@sunriselab.com wrote: Is there any information available that would indicate the effect of decolorizing an HE stained slide on subsequent IHC staining / antigenicity? Thanks, Laurie Laurie Best,BS Histology and Cyto Prep Supervisor Sunrise Medical Laboratories 250 Miller Place Hicksville, N.Y. 11801 tel (631)435-1515x1018 fax(631)435-2014 This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy or take any action in reliance on it. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] On the lighter side...
40 blessed years that I would not trade for anything. I have met so many wonderful people (well, maybe a few not so wonderful...) and have learned so much. The good Lord has blessed me with a talent that I feel has allowed me to make a contribution to society. On Friday, August 8, 2014, White, Lisa M. lisa.whi...@va.gov wrote: 15 years as HT...26 years total in medicine started as an EMT and after some time discovered that patients in a jar is a blessing J thus the transfer to Pathology..and that is the rest of the story. Lisa White, HT(ASCP) Classification: Internal and External Use\\Not VA Sensitive This message has been categorized by White, Lisa M. on Friday, August 08, 2014 at 7:32:08 AM in accordance with VA Handbook 6500 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu javascript:; http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jim Burchette Fly Fishing Bum *'(((* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: bunsen burner at the embedding center
Back in the 70's we would boil metal base molds in water using a bunsen burner and a 3 legged ring stand. On Sep 20, 2013 9:37 AM, Davis, Cassie cda...@che-east.org wrote: Hi Valerie, When I started in Histo in 90' everybody used the alcohol burners...Open flame concern became a concerned and the separate forcep warmers were purchase because the old embedding centers did not have the nice warmers like the new ones do. The last place I worked at had an old embedding center when I started but we weren't allowed open flames. Fortunately, we found an unused Bacteria Incinerator that Micro. wasn't using and used that until that embedding center died. That worked great! Cassandra Davis cda...@che-east.org 302-575-8095 From: Hannen, Valerie valerie.han...@parrishmed.com To: Histonet Post (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Sent: Thursday, September 19, 2013 11:04 AM Subject: [Histonet] Bunsen Burner Hi all.. We are having a discussion/ disagreement in our department as far as whether using a bunsen burner at the embedding center is against fire codes. What is the consensus?? Thanks, Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] immunohistochemistry help please
Skip the buffer and go in the hematox straight from water. See if that works. JB On Aug 1, 2013 3:20 PM, Tarantelli, Rebecca Anne ra...@pitt.edu wrote: Hi all, I am new to this forum, but a friend recommended I send my issue out to you for help. Four weeks ago I completed a new protocol for Immunohistochemistry (IHC) using protinase K epitope retrieval, and monoclonal antibody 3F6. It worked perfectly. Three days later, after 13 beautiful stains, it just stopped working. The slides will not even take up the counter stain - Hematoxylin. I have exchanged my xylene, and tried again, and it still is not working. I am guessing since my counter stain isn't working, this is an issue in the deparaffinization stage, but I'm just not sure. Please let me know if you have any suggestions! Thank you Rebecca Rebecca Tarantelli ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] making 10 % EDTA
Don't forget EDTA doesn't fully go into solution until the pH approaches 8.0. You will need to prepare a sodium hydroxide solution for pH adjustment. On Wed, Jun 26, 2013 at 3:46 PM, Goins, Tresa tgo...@mt.gov wrote: The molecular weight of the EDTA will vary depending on chemical makeup - regardless, the solution you have is probably less than 10% already. EDTA tri-sodium salt with MW 358 at 0.25 M is 89.5 g per liter or 8.95 g per 100 ml or 8.95%. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle Sent: Wednesday, June 26, 2013 12:37 PM To: histonet Subject: [Histonet] making 10 % EDTA Hi Histonetters, I have purchased a liquid EDTA solution that is .25M. I need to make 10% EDTA. Do I just dilute it 1/10 with DI water? Thanks Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 Pager#5029 (410) 328-5558 (410) 328-5508 fax https://cf.umaryland.edu/freezer/promo_pbr.cfm This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jim Burchette Fly Fishing Bum *'(((* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
