Re: [Histonet] Histonet Digest, Vol 159, Issue 24
Hi Allison, I talked to my safety guy and there isn't anything specific. They are telling you that because there is a fire hazard warning on formalin cubes, but we know that the change of it catching fire is slim to nil. They want you to use the same standards as with alcohol and xylene (1 gallon per 100 square feet). It is all so dependent on who you have inspecting you and what their background is. Joanne Clark, HT Pathology Consultants of New Mexico -- Message: 1 Date: Mon, 27 Feb 2017 19:26:54 + From: "Eck, Allison" <a...@dh.org> To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] square footage for formalin Message-ID: <4ed8c96a8f20fc4f883a92e2a0a0d64a97341...@dh-mail01.dhorg.org> Content-Type: text/plain; charset="us-ascii" Good afternoon, We have inspectors here and they are questioning the size of the room in the operating room where they keep their 5 gallon cube. Does anyone know of any square footage requirements for a room that where formalin is kept and used? Thank you in advance Allison Allison Eck, HTL(ASCP)cm,QLS, AHI(AMT) Lead Tech Histology Doylestown Hospital 595 W State St Doylestown, PA 18901 215-345-2264 a...@dh.org<mailto:a...@dh.org> -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histonet Digest, Vol 159, Issue 20
Hi James, most processors come with wiring for hookup to an alarm system. We just purchased one from Radio shack, hooked the external wiring from the processors into it, hung it on the wall behind the units,then programmed the phone numbers to call into the alarm system. It's fairly old now, but it's a Radio Shack Security Auto Dialer. Joanne Clark, HT Director of Histology Pathology Consultants of New Mexico Message: 11 Date: Thu, 23 Feb 2017 17:27:14 + From: "Fortune, James A." <james.fort...@unitypoint.org> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] External alarm system for VIP 6 Message-ID: <bn6pr11mb19544629a267bf2cf320958692...@bn6pr11mb1954.namprd11.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" Hello all, We are looking to purchase an external alarm system for our 2 vip 6 processors. I called Sakura to see if they sold them or if they had any recommendations as far as which ones are compatible with the VIP and got nowhere. So I am asking all of the histonet world for any recommendations on external alarms you have used or purchased. Thanks in advance! James (Andy) Fortune Histology Tech Specialist Meriter Health Services Madison, WI This message and accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. sections 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GLX Linnistainer
Greetings Histonetters, we recently purchased a refurbished GLX Linnistainer that has an 8 minute stain time. I was wondering if any of you out there use the same instrument and can give me some insight on your reagent sequence and what type of Hematoxylin and Eosin your using. Thank you! Joanne Clark, HT Director of Histology Pathology Consultants of New Mexico Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] p16
Good Morning Histonetters, I am wondering how all of you using p16 from Roche on a Leica staining system are going to adapt to the changes coming from Roche. Are you going to pay the exorbitant price for the new kit and list the test as an LDT in your reports, or are you going to source one of the ASR antibodies out there and use that route? I just want to be sure I can re work a research antibody and report it as an ASR test in our reports without CAP giving us a hard time about it. Thanks for the feedback. Joanne Clark, HT Director of Histology Pathology Consultants of New Mexico Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] ER/PR Uneven Staining
It's rather strange. There are positive tumor cells throughout the needle core, but the bottom third is staining very strongly all across the core, then there is a small portion where the staining is there but not as strongly as the section below it, then another segment above this where the staining is strong again. The strong and weak staining is across the entire core of the tissue, not just at the edges. -Original Message- From: Cartun, Richard [mailto:richard.car...@hhchealth.org] Sent: Tuesday, November 29, 2016 3:12 PM To: Joanne Clark Cc: histonet@lists.utsouthwestern.edu Subject: RE: ER/PR Uneven Staining Can you provide more details about the uneven immunoreactivity? Are the negative cells located at the periphery of the specimen or are the negative and positive tumor cells mixed together throughout the specimen? Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -Original Message- From: Joanne Clark via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, November 29, 2016 4:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ER/PR Uneven Staining I had a breast needle core today that when stained with ER and PR the staining was uneven throughout the core, even though the cancer cells were present in the entire core. The specimen had 10 hours fixation in 10% NBF. I could understand the uneven staining from inadequate fixation on large grossed in breast tissue, but 10 hours with needle core biopsies has always been more than sufficient. Does anyone have any ideas? We use Leica's ER and PR antibodies on the BOND. Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ER/PR Uneven Staining
I had a breast needle core today that when stained with ER and PR the staining was uneven throughout the core, even though the cancer cells were present in the entire core. The specimen had 10 hours fixation in 10% NBF. I could understand the uneven staining from inadequate fixation on large grossed in breast tissue, but 10 hours with needle core biopsies has always been more than sufficient. Does anyone have any ideas? We use Leica's ER and PR antibodies on the BOND. Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Competency for Supervisors
Good Morning Histonetters, how do all you histology supervisors out in Histoland do competency on yourselves? Do any of you have a good form to evaluate your performance both on the bench and as an administrator? Anything will be of assistance. Thank you! Joanne Clark, HT(ASCP) Director of Histology Pathology Consultants of New Mexico 575-622-5600 Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CAP ANP.22970 Query
Hi Histonetters, we are wondering what everyone else out there is doing to be compliant with the following requirement? We do ER and PR by IHC but dont know what published benchmarks are out there to compare ourselves to. Also, how do you record interobserver variability amongst the pathologists? Any insights into this would be appreciated. ANP.22970 Annual Result Comparison Phase II For immunohistochemical and FISH/ISH tests that provide independent predictive information, the laboratory at least annually compares its patient results with published benchmarks, and evaluates interobserver variability among the pathologists in the laboratory. NOTE: Individuals interpreting the assay must also have their concordance compared with each other and this concordance should also be at least 95%. With specific reference to estrogen and progesterone receptor studies: in general, the overall proportion of ER-negative breast cancers (invasive and DCIS) should not exceed 30%. The proportion is somewhat lower in postmenopausal than premenopausal women (approximately 20% vs. 35%). The proportion is considerably lower in well-differentiated carcinomas (<10%) and certain special types of invasive carcinomas (<10% in lobular, tubular, and mucinous types). The proportion of PgR-negative cases is 10-15% higher than for ER-negative in each of these settings. Investigation is warranted if the proportion of negative cases is significantly lower in any of these settings. Joanne Clark, HT Director of Histology Pathology Consultants of New Mexico Roswell, New Mexico Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] JACHO
Hi Histonetters I have a question for those of you who have JACHO accreditation. Part 5 of QSA.13.04.01 states that when a nonpathologist performs gross analysis under the supervision of a qualified pathologist, that the individuals work is reviewed and documented within 24 hours. My question is how are you confirming and documenting this for specimens grossed in on Friday by the grossing tech that will not be read by the pathologist until Monday? Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PAS Stain
Hi Histonetters, I'm hoping someone can help me troubleshoot our PAS diastase method. We have been digesting the tissue in 0.5% diastase of malt in a 60 degree oven for 30 minutes, but do not see the glycogen being digested out. I have tried alpha amylase and beta amylase also without any luck. Does anyone have any suggestions to get the digestion to work Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] H. pylori by IHC
Recently someone placed a query regarding the precipitate with Cell Marques H. pylori marker. The response was to filter it before use to clean it up. May I ask how it is being filtered? We have the same problem and it makes the slide difficult to interpret. Joanne Clark, HT BAAS Director of Histology Pathology Consultants of New Mexico Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Foreign Trained Tech
Tim is absolutely right. I am a Canadian trained tech and before I could work in the US I had my credentials evaluated by WES (you can google them to get contact info) to see which ASCP exam I qualified for. Joanne Clark, BAAS, HT Director of Histology Pathology Consultants of New Mexico -- Message: 3 Date: Fri, 12 Feb 2016 10:18:32 -0500 From: Charles Riley <cri...@dpspa.com> To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fwd: Natalia's Credentials Message-ID: <CAAQhB13ERU+HVgJW75Peos6EdmBF2q5ez4eQ=zcbjjkfew0...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 My lab is looking to hire a tech who has been trained in another country. Management wants to know if their credentials qualify them to work in a CAP accredited lab without direct supervision or if they will need to sit for the ASCP board exam. If they need to sit for the exam do her credentials qualify her to take the exam now? Her credentials are listed below Ministry of Public Health & Social Care Diploma Graduated the full course of education at Institute of Health Care Employees with Secondary Professional Education Bulgarian Medical Academy. Qualification of Clinical Laboratory Assistant Transcript includes: Laboratory Equipment and Laboratory Appliances Clinical Laboratory Histology with General Pathology & Histological Technique Biochemistry 120 hours of Histological Laboratory -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE -- Message: 4 Date: Fri, 12 Feb 2016 15:49:09 + From: "Morken, Timothy" <timothy.mor...@ucsf.edu> To: Charles Riley <cri...@dpspa.com> Cc: Histonet <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Fwd: Natalia's Credentials Message-ID: <761e2b5697f795489c8710bcc72141ff6fd16...@ex07.net.ucsf.edu> Content-Type: text/plain; charset="us-ascii" Charles, Foreign course work is useless for CLIA until it is vetted by a transcript service that can evaluate the validity of the school and how that course work matches with the required typical US courses. ASCP requires such vetting for foreign applications for ASCP certification (I've been involved with a few people taking that route). It can take weeks to months to get such verification depending on the bureaucracy involved in the foreign country. And the applicant has to pay for it. Experience counts, but you don't know what the quality of that is either until you see her in action. If this person is already in the US and local to you, and you still want to pursue it, I suggest hiring her as a lab assistant with the idea you would evaluate her skills on site, if you are willing to do that. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Friday, February 12, 2016 7:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fwd: Natalia's Credentials My lab is looking to hire a tech who has been trained in another country. Management wants to know if their credentials qualify them to work in a CAP accredited lab without direct supervision or if they will need to sit for the ASCP board exam. If they need to sit for the exam do her credentials qualify her to take the exam now? Her credentials are listed below Ministry of Public Health & Social Care Diploma Graduated the full course of education at Institute of Health Care Employees with Secondary Professional Education Bulgarian Medical Academy. Qualification of Clinical Laboratory Assistant Transcript includes: Laboratory Equipment and Laboratory Appliances Clinical Laboratory Histology with General Pathology & Histological Technique Biochemistry 120 hours of Histological Laboratory -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://cp.mcafee.com/d/FZsS938OrhovvpKyqemkPtPqdQnCnNPUVYSzt5VBYsUCyrhKyYO-ev7e6QrLIe8IcTd79IhIvIiEamJ1nBPqREaYKrIC-DV1N_HYUMCyeovWZOWqdNT8IIICzBxZPG8FHnjlLtPBgY-F6lK1FJ4srjKrLOqbaab0VZx4TsS03fBiterDiHsOpzWj-ceZ1L1dnoovaAVgtHxel9R1FIDaAWwQKgGT2TQ1hYGjFPsWlrCjcvivNxTEdCQhRPhOCr2YGjG3jh07i8_io1Cy0cRfd42b0U0Ph06QETVEwhjdII6WlYMYvib5NlI -- Message: 5 Date: Fri, 12 Feb 2016 17:05:14 + From: Rayen Gonzalez <rgonza...@centraltexasgi.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Cc: Lin Bustamante <lbustama...@centraltexasgi.com> Subject: [Histonet] Need Slide Cabinets Message-ID: <0a9da3075e1940f081b052fcd22bd...@exchange.ctgi.local> Conte
[Histonet] Manual Cassette Labelling
Hi Histoland, We have a satellite lab that manually prepares their cassettes. We have had significant problems with the markers coming off after tissue processing. We have tried StatLab markers, Leica Markers and the red AquaRellable pencils and continue to have issues. Other than purchasing a cassette labeling system (their volumes are pretty low and there is limited bench space in the lab for a cassette labeler) does anyone have any suggestions or ideas on what might be causing this phenomenon or a really good permanent lab marker that has been successful for you? Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Breast Fixation
I would do a delayed start on your tissue processor Friday night to include the extra two hours you need of fixation time and just have the run come off two hours later on Saturday morning. Just adjust the hours of your per diem Saturday tech to come in later. Joanne Clark, HT Director of Histology Pathology Consultants of New Mexico Message: 4 Date: Wed, 29 Jul 2015 08:01:31 -0700 From: Heckford, Karen - SMMC-SF karen.heckf...@dignityhealth.org To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: [Histonet] Breast fixation Message-ID: d329219d3b967447a9e3ed3242117e3206fedc7...@phx-msg-007-n1.chw.edu Content-Type: text/plain; charset=us-ascii Good Morning, I have a question about breast fixation. I am in a little bit of a pickle with the 6-72 hour rule for the fixation on breast tissue. Friday I am getting 2 breast cases in the afternoon and both will not have the required minimum 6 hour formalin fixation for my per diem to cut early Saturday morning. He will not be able to make it in again until Monday night. The tissue will be about 3-4 hours (this includes time on the processor) over the 72 hour maximum.Does anyone have any suggestions on what can be done? We are a one person show here. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. -- Message: 5 Date: Wed, 29 Jul 2015 12:10:19 -0300 From: Emily Dewar emilydewar32...@gmail.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NetWell inserts and IHC with TUNEL stain Message-ID: CAJ7MPPp=h9pgEkH-SxJG=KkDiDxi4=6_ttrap7rcb5nsjnm...@mail.gmail.com Content-Type: text/plain; charset=UTF-8 Hello, I will be performing a number of immunos within the next couple of months, and have been wondering what the best way to do so might be with no tissue damage during the process. Does anyone know whether using NetWell inserts for immunos to transfer tissue affects morphology? The tissue will be placed into the insert, and submersed in solution within a well plate to be incubated on a shaker. The problem is that contact with the NetWell insert could damage the tissue, not only with the rocking, but with transfer from one solution to another. I am under the impression that with TUNEL staining, is often difficult to differentiate whether the damage in cells caused by handling/extraction, or from the treatment itself. While not impossible, I will not have the time to develop or perform such a procedure. If anyone has any knowledge or insight, it would be greatly appreciated. Thank you for your time, Emily Dewar Laboratory Technician -- Subject: Digest Footer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://cp.mcafee.com/d/k-Kr6h8idEIcL8CzB55AsrKrhKyYO-ev7fCQrELcLzD4QjqdQnCnNPUVMSztZxN5xCVEVdydzZyl1iREaYKrmJ1nBPqavTd791Z_HYCyCO-esWZOWqbz7cccT7cfsJt6OaaJQSel3PWApmU6CS3r1K_9Tso73xParNKVI06vaAWsTeBmVAP7QDYotW3u2qKMM-l9OwXn2sGjG3jpel9R1FsxlK5LE2zVkDjCVQGTcCo-A_z3LgrdLcee6MLaAWwQQg1QyfQC0pEw3djPh0yNs0Ph06QETVEwphdII6N00eEd9Y -- End of Histonet Digest, Vol 140, Issue 31 * Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Assistant Supervisor Job Available
Hi All, we have a position available for an Assistant Supervisor in Roswell, located in sunny southeast New Mexico. All routine histology skills will be needed (processing, embedding, cutting, special stains, frozen sections, etc.). They would be responsible for the smooth running of the histology lab in my absence and would rotate with me on immunohistochemistry and grossing bench. The successful candidate will need to have a minimum of an Associate's Degree in Science (to be able to gross) and ASCP certification. Grossing experience would be a definite asset as would supervisory experience, but we would be willing to train the right candidate. Joanne Clark, BAAS, HT Director of Histology Pathology Consultants of New Mexico Roswell, New Mexico 575-622-5600 x 221 Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 137, Issue 15
She can be here in two weeks. Sent from my iPhone On Apr 12, 2015, at 11:01 AM, histonet-requ...@lists.utsouthwestern.edu histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://cp.mcafee.com/d/avndxMwcCQm4TDDTHzDztPqdQnCnNPUVYSzt5VBYsUCyrhKyYO-ev7e6QrLIe8IcTd79IhIvIiEamJ1nBPqREaYKrZ1ztjvvW_9L6zBdAQsCzDHTbFThsVYsed7arb_bnjIyyHtdDBgY-F6lK1FJ4SYrLRQkhPz0WXVEVdTdw0PVkDjCVQGTcCo-A_z3LgrMjlS67OFek7qUjBitgqr9OFeEdbAaJMJZ0kvaAWsTeBmVAP7QDYotW3pJyXzNIbOFeEdd40t8zZ9w6q80PkYQg8Izw3d40rizvCy15YSOMrgo0Td3SY or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Pigs as models of human biology (Jorge A. Santiago-Blay) -- Message: 1 Date: Sun, 12 Apr 2015 09:45:20 -0400 From: Jorge A. Santiago-Blay blayjo...@gmail.com Subject: [Histonet] Pigs as models of human biology To: histonet@lists.utsouthwestern.edu Message-ID: cagbdduam+psofrstxunsksso8u88eqtjq4aqur-dfwraskz...@mail.gmail.com Content-Type: text/plain; charset=UTF-8 Dear Histonetters: I often wonder what are the reasons why pigs seem to be used so often in studies of human physiology. For phylogenetic reasons, I would have thought chimps would be the preferred choice. Is it because of humane, $, or are there other considerations? Thank you. If you know, please send me an email to: blayjo...@gmail.com Sincerely (and apologies if you have received this message more than once), Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://cp.mcafee.com/d/1jWVIp6jqb2rPPXRNPNKVJ6WbPbUVYs-rhKyYO-esjhdEThupv7fzD3qdTS74m6rCzAS8SfS9k5bmwHOVJqQ5und-wNKFLLZvATzhOCOqejhPRXBQXEKs-e76zBdB_BHFShhlKCPOEuvkzaT0QSCrudTWWa8VNwttYQsCXCM0p0_iOPp0-l2vNFO-j-AVaRxbjWor9OFeEdbAaJMJZ0kvaAWsTeBmVAP7QDYotW3pJyXzNIbOFeEdd40t8zZ9w6q80PkYQg8Izw3d40rizvCy15YSOMrkCZ2 2. Free examples of papers published in *LEB*: http://cp.mcafee.com/d/k-Kr6jqb2rPPXRNPNKVJ6WbPbUVYs-rhKyYO-esjhdEThupv7fzD3qdTS74m6rCzAS8SfS9k5bmwHOVJqQ5und-wNKFLLZvATzhOCOqejhPRXBQXEKs-e76zBdB_BHFShhlKCPOEuvkzaT0QSOrudTWWa8VNwttYQsCXCM0p0_iOPp0-l2vNFO-mSvAqNboJ7PcGJQbOFhLWor9OFeEdbAaJMJZ0kvaAWsTeBmVAP7QDYotW3pJyXzNIbOFeEdd40t8zZ9w6q80PkYQg8Izw3d40rizvCy15YSOMrT2tf. 3. *Guidelines for Authors* and page charges of *LEB*: http://cp.mcafee.com/d/avndy0Ad1MOrhojuuvuKeudTdEThupv7fzDPqdQnCnNPyq9J6WbPbUVYsUrhK-MUyMPsQsCN6N-NawFqQ5undHmwHOVLQ6dRdZ_HYCYqekSjhOqeuLsKDt5PDNMUQsFILYJteOaaJQSul3PWApmU6CS3rNK_nhh7ec3HLCzATsS0387Wmmr87OEj-denPoS9Oc_j3pel9R1FsxlK5LE2zVkDjCVQGTcCo-A_z3LgrdInsudxul9R1FEw3F4vFc0Ph06qDCy15As0pEw3qkrYQg8LCSm3sTnDDp-e *.* 4. Want to subscribe to *LEB*? http://cp.mcafee.com/d/avndxNJ5xdVVZWUVUTsSzt5VBYs-evdEThupv7e9ECQrELcLzDNPxJ6XX3yb3dPhOr4r7X4G2BHglVsSJq2LbC_goTkTT-LOrNEVjpd79EVWZOWtQnev73zhOCO_ORQX8EGTjpVkffGhBrwqrvdL6XZt54sUMeK-qejtPo0cwvFppIwvaxfUQVvadAamz9qsGMFxIDaAWwQKgGT2TQ1hYGjFPsWlrCjcvivNxTEdCSbKf6MLaAWwQQg1QyfQC0pEw3djPh0yOe0cQg1Jad-q84nPrb1Ie9qtDdO http://cp.mcafee.com/d/5fHCNAq3x8SyMCYY-ZssYrKrhKyYO-ev7fCQrELcLzD4QjqdQnCnNPUVMSztZxN5xCVEVdydzZyl1iREaYKrmJ1nBPvEcrGrX_nVdUQsFICzAQsZuVteWbDfzxNEVjpvVqWtAklrFIYG7DR8OJMddK6Tzt-Kyyeso7nvd79KVI06gfQI8w0vUNma2QvFjBYdAVkDk6BO5mUm-wafBiterDiHsOpzWj-ceZ1ISNtNUS5VkDk6Cy0eAh-AM3d40pGuq84mhM1Cy0dFhLPh0y-rpodPZkVyc_Y http://cp.mcafee.com/d/FZsS86Qm4TDDTHzDztPqdQnCnNPUVYSzt5VBYsUCyrhKyYO-ev7e6QrLIe8IcTd79IhIvIiEamJ1nBPqREaYKrZ1ztjvvW_9L6zBdAQsCzDHTbFThsVYsed7arb_bnjIyyHtdDBgY-F6lK1FIsrudTWWa8VNwttYQsCXCM0qSD_kxaFZi9rGiXQ6YFfnnsB12hCi3uDQOCMDbidi1-BKIWGr9OFeEdbAaJMJZ0kvaAWsTeBmVAP7QDYotW3pJyXzNIbOFeEdd40t8zZ9w6q80PkYQg8Izw3d40rizvCy15YSOMrgNgo -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://cp.mcafee.com/d/2DRPos83gwrhojuuvuKeudTdEThupv7fzDPqdQnCnNPyq9J6WbPbUVYsUrhK-MUyMPsQsCN6N-NawFqQ5undHmwHOVLQ6dRdZ_HYCYqekSjhOqeuLsKDt5PDNMUQsFILYJteOaaJQSul3PWApmU6CTzrNK_nhh7ec3HLCzATsS03fBiterDiHsOpzWj-ceZ1L1dnoovaAVgtHxel9R1FIDaAWwQKgGT2TQ1hYGjFPsWlrCjcvivNxTEdCSbKf6MLaAWwQQg1QyfQC0pEw3djPh0yOe0cQg1Jad-q84nPrb1J67KLKDS2LLj End of Histonet Digest, Vol 137, Issue 15 * Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material
[Histonet] RE: Histonet Digest, Vol 129, Issue 14
I have been a histotech for 28 years. The first 20 with my MLT (Canadian certification)and the last 8 here in beautiful and warm New Mexico with my HT. Completed my Bachelor's last year, so hopefully I can get my HTL within the next year or two. Joanne Clark, BAAS, HT Director of Histology Pathology Consultants of New Mexico Message: 2 Date: Fri, 8 Aug 2014 16:45:03 + From: Morken, Timothy timothy.mor...@ucsfmedctr.org Subject: RE: [Histonet] On the lighter side... To: 'Beth Brinegar' bbrinegar...@gmail.com, Elizabeth Chlipala l...@premierlab.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu,Cartun, Richard richard.car...@hhchealth.org Message-ID: 761e2b5697f795489c8710bcc72141ff36791...@ex07.net.ucsf.edu Content-Type: text/plain; charset=utf-8 Yah! A new one!!! Stay on Histonet, go to meetings and stay interested- the burnout prevention! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Friday, August 08, 2014 8:01 AM To: Elizabeth Chlipala Cc: histonet@lists.utsouthwestern.edu; Cartun, Richard Subject: Re: [Histonet] On the lighter side... 3 years in histology, 2 years registered! Beth Brinegar HTL (ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 On Fri, Aug 8, 2014 at 9:53 AM, Elizabeth Chlipala l...@premierlab.com wrote: Too funny Richard - you would pass with flying colors For me its 31 years - HTL-930 I have really been blessed, I love my job and I have really enjoyed my career. Every day there is something new to learn or to work on, for example the lab is putting the final touches on a poster that will be displayed at NSH this year, working on that has been exciting and a lot of fun too. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax l...@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: histonet-boun...@lists.utsouthwestern.edu [ histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard [ richard.car...@hhchealth.org] Sent: Friday, August 08, 2014 8:32 AM To: Douglas Porter; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 36 years; however, not registered. Hopefully, I can take the QIHC someday and pass. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Senior Histotech Position
We have a position open for a Senior HT/HTL, preferably with grossing experience for our Las Cruces lab. Interested parties can contact me directly for details. Joanne Clark, BAAS, HT(ASCP) Director of Histology Pathology Consultants of New Mexico 575-317-6403 jcl...@pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Clia grossing regulations
Carol, here they are: **REVISED** 07/11/2011 ANP.11610 Gross Examination Qualifications Phase II If individuals other than a pathologist or pathology resident assist in gross examinations, such individuals qualify as high complexity testing personnel under CLIA regulations. NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under CLIA regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, NAACLA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. It is the responsibility of the laboratory director to determine whether an individual's education, training and experience satisfies the requirements of this checklist requirement. This checklist requirement applies only to laboratories subject to US regulations. Joanne Clark, HT Director of Histology Pathology Consultants of New Mexico -- Message: 4 Date: Wed, 20 Nov 2013 21:22:47 -0500 From: Carol Kowalcyk ckowal...@gmail.com Subject: [Histonet] what are the Clia regulations for an Ht to gross??? To: Histonet@lists.utsouthwestern.edu Message-ID: 351ea433-cfda-4d7d-b169-60c4a1483...@gmail.com Content-Type: text/plain * Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Biopsy Problems
Hi Fellow Histonetters, we have started having this problem with our G.I. biopsies, and not all of them, just the odd one here and there. After staining, when looking at it on the scope, you can't get it to focus in one plane. It's not cutting artifact, because we have recut the block making sure it is well cooled etc. before picking up and staining the section. The cells, when in focus, look fixed and properly processed, and the staining is the way it should be. I have no idea what is causing this artifact. Has anyone else seen this and can shed some light on what is causing it? Joanne Clark, HT Director of Histology Pathology Consultants of New Mexico Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cyclin D1
Is anyone running Cyclin D1 on the Leica Bond? I have their ready to use antibody, but it hasn't been worked up on the Bond and my first try has left me with more background than I would like. Does anyone have it worked up with a protocol they would like to share? Joanne Clark, HT(ASCP)CM Director of Histology Pathology Consultants of New Mexico Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Submitting Questions
I bet that's it. I re-submitted my question and took out all the logo's and fancy stuff after my name. Hopefully it will now appear. -Original Message- From: Douglas Porter [mailto:doug.por...@caplab.org] Sent: Wednesday, September 25, 2013 10:20 AM To: 'Nails, Felton'; 'nancy lowen'; Joanne Clark; histonet@lists.utsouthwestern.edu; 'Martha Ward-Pathology' Subject: RE: [Histonet] RE: Submitting Questions I have .jpg files in my signature for initial emails but not in my reply emails. I sent a test message yesterday and got the following reply from the server: Your mail to 'Histonet' with the subject Test Message! Is being held until the list moderator can review it for approval. The reason it is being held: Message body is too big: 119656 bytes with a limit of 60 KB Either the message will get posted to the list, or you will receive notification of the moderator's decision. If you would like to cancel this posting, please visit the following URL: http://lists.utsouthwestern.edu/mailman/confirm/histonet/9abe483614aeb7cc29e e5aa890b57bc164701b31 I suspect the .jpg files are what is causing the problem as the message was only Test Message! I will strip out the .jpg files and try again. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.por...@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Wednesday, September 25, 2013 12:05 PM To: 'nancy lowen'; 'Joanne Clark'; histonet@lists.utsouthwestern.edu; Martha Ward-Pathology Subject: RE: [Histonet] RE: Submitting Questions I have even tried copying the following link (histonet@lists.utsouthwestern.edu) and pasting it and I'm still unable to make a post. -Original Message- From: nancy lowen [mailto:clayca...@yahoo.com] Sent: Wednesday, September 25, 2013 10:49 AM To: Nails, Felton; 'Joanne Clark'; histonet@lists.utsouthwestern.edu; Martha Ward-Pathology Subject: Re: [Histonet] RE: Submitting Questions Me too! On Tue, 9/24/13, Martha Ward-Pathology mw...@wakehealth.edu wrote: Subject: [Histonet] RE: Submitting Questions To: Nails, Felton flna...@texaschildrens.org, 'Joanne Clark' jcl...@pcnm.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Tuesday, September 24, 2013, 12:54 PM I have had the same problem over the past few months... Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Tuesday, September 24, 2013 2:58 PM To: 'Joanne Clark'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Submitting Questions I am in the same situation??? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Tuesday, September 24, 2013 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Submitting Questions Can anyone out in histoland explain to me why my submitted questions are not making it through to histonet? I submitted a question on the 19th of September, and I still have not seen it appear in the group emails I get daily. I sent the question to the correct address (I checked it to be sure). When I respond to someones question, I can see my responses, I just can't seem to get an original question submitted. Can anyone tell me what I am doing wrong? Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet
[Histonet] Submitting Questions
Can anyone out in histoland explain to me why my submitted questions are not making it through to histonet? I submitted a question on the 19th of September, and I still have not seen it appear in the group emails I get daily. I sent the question to the correct address (I checked it to be sure). When I respond to someones question, I can see my responses, I just can't seem to get an original question submitted. Can anyone tell me what I am doing wrong? Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 111, Issue 7
Hi Kathy, we do p16 on the Leica Bond. You have to purchase the p16 from
Ventana, they are the only one who has it. It comes as a predilute and we use
it as is with the DAB Refine Detection using retrieval solution ER1 for 10
minutes.
Joanne Clark, HT
Histology Supervisor
Pathology Consultants of New Mexico
-
Message: 21
Date: Wed, 6 Feb 2013 09:06:34 -0800 (PST)
From: Kathryn Maddox katemad...@yahoo.com
Subject: [Histonet] P16 on Leica Bond
To: histonet histonet@lists.utsouthwestern.edu
Message-ID:
1360170394.17396.yahoomail...@web163404.mail.gq1.yahoo.com
Content-Type: text/plain; charset=iso-8859-1
If anyone out there is performing? p16 on the Leica Bond immunostainer, could
you please tell me where you purchase your antibody and what protocol you are
using?
Thanks so much!
Kathy Maddox HT{ASCP}
Lake Charles, Louisiana
--
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End of Histonet Digest, Vol 111, Issue 7
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[Histonet] DAKO Her2
Hi All, sorry for how I am submitting this but I have been sending in questions to the address provided by nothing is going through. I would like to know who uses DAKO's IVD Her2 IHC marker and how it works. We want to start running this on our Leica BOND and any info would be much appreciated. Thanks Joanne Clark, HT(ASCP) Histology Supervisor Pathology Consultants of New Mexico Roswell, NM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Metal Molds
We occasionally use metal molds and we clean them after every use. We do this by putting them into the tissue processor with the dirty rack during the clean cycle. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico -- Message: 9 Date: Mon, 8 Oct 2012 14:31:45 -0600 From: O'Donnell, Bill billodonn...@catholichealth.net Subject: [Histonet] Metal molds To: histonet@lists.utsouthwestern.edu Message-ID: 4940df6d1c5fdf48931b6966aaef93957a3...@chimsx08.chi.catholichealth.net Content-Type: text/plain; charset=us-ascii OK folks, I know I should be smarter than this and I haven't seen discussion on it lately Are people cleaning their metal embedding molds after evey embedding session? If not, how often do you clean them? Do you clean them at all? If you clean them, how do you do it? Thanks Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 102, Issue 24
We used to have this problem too, till we switched to Tru-Bond slides from Tru-Scientific. Our contact is s...@tru-scientific.com . We also try to dry our slides for 1 hour in the 60 degree oven whenever possible and especially for really fatty tissues, like breast. We haven't had any problems with antigen integrity. I think greater than 60 could cook your tissue and affect things, but if you make sure it doesn't go any higher you should be OK. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico -- Message: 4 Date: Mon, 21 May 2012 09:25:21 -0500 From: Brendal Finlay brendal.fin...@medicalcenterclinic.com Subject: Re: [Histonet] Help To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: ea0c3755f29a151442a3fddc720e7...@medicalcenterclinic.com Content-Type: text/plain; charset=utf-8 Nancy, We've had similar issues with fatty tissue falling off of the slides while performing IHC.?? We use Superfrost + slides which we have found to really hold the tissue well.?? Also, I have learned through reading round on the??Histonet??that air drying doesn't completely remove the water from the middle area of a tissue section.?? For this reason, we no longer air dry at all unless it's a slide that was cut the day before and just happened to be air dried.?? Our protocol changed to cutting the slides and draining them well, then putting them in a 60 C oven for 15 minutes.Then??the slides are run??down to water on an automated stainer with another 15 minute time in the oven on the stainer.?? A specific instance??when the tissue falls off,??was during antigen retrieval in Trilogy in a pressure cooker.?? If the pressure was manually released, this would cause the Trilogy to boil??and??it would separate the tissue from the slide.?? Ourprotocol changed to 12 minutes in the pressure cooker with Trilogy, then around 8 minutes??to wait for the pressure to release on it's own.?? We would then rinse softly in distilled water to remove the??Trilogy.?? This also seemed to help with the issue. ?? The combination of this??has worked??fairly well for us with some exceptionally stubborn tissue still??attempting to fall off of the slides.I would love to??hear of other's experiences and??how they resolved this. I do wonder about the length of time in your oven.?? I had spoken with one of our Biocare reps about this when we encountered the problem and he felt that longer than 30 minutes in the oven would damage the specimen's IHC integrity.?? Brendal Finlay HT (ASCP) Original message- From: Cloughley-Gray, Nancy cloughl...@rvh.on.ca Date: Fri, 18 May 2012 14:02:35 -0500 To: 'histonet@lists.utsouthwestern.edu'histonet@lists.utsouthwestern.edu Subject: [Histonet] Help I'm a Histotechnologist working in the Regional Hospital in Barrie, ON Canada. We are using the Ventana Ultra for our Immunohistochemistry (IHC). Since the end of February, we have been having issues with some tissues lifting off our positive (marked with +) charged slides. It seems to be mostly with the fatty and/or larger sections. We now dry our slides for one hour at room temperature (R.T.) and an additional hour at 60 degrees C. We cut our IHC sections at 4 um. Since we have tried 2 different types of + slides and will be trying another type of charged slide (from Newcomer this time) I was wondering if anyone has any other suggestions? I also have another question regarding a QC (quality control) issue. We use a multi-tissue control that is applied to the top of all our test slides for IHC. One of our paths commented that there is some positive staining in the smooth muscle nuclei of thenormal bowel when we are testing for Progesterone (PR). We are using a Heat Induce Epitope Retrieval (HEIR) of 36 minutes with CC1 (Ventana's proprietary buffer @ pH of 8.0-8.5) and a primary antibody incubation time of 16 minutes with PR clone 1E2. (Ventana instrumentation provides pre-diluted antibodies and the user adjusts the concentration of the antibody by adjusting the time the primary antibody is incubated with the tissue). I am concerned about the implications of this staining and I have not been able to find a reference to this kind of unusual staining pattern. The bowel tissue that we are using as QC is from a 62 year old female patient. I was wondering if anyone has had any experience with this kind of staining and /or any references that I could use. Thanking you in advance, I look forward to your input, Nancy Cloughley-Gray MLT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Qualifications for grossing
David, after reading your post I was not at all surprised to see that you are a PA. I am assuming that explains your vitriol towards techs that gross. Yes, CLIA does provide the educational requirements for high complexity testing, but what on earth makes you think that a tech with the proper CLIA qualifications can gross without proper training by a pathologist? CAP requires that as well as extensive documentation of training AND a list of the specimens approved by the Lab Director that a 'non-pathologist' is allowed to gross. I'm sure you can tell that I am a Histotech with an Associates Degree and I do the grossing in my lab. I can assure you that I do a good job and if there is EVER any question regarding how to gross in a specimen I will get a pathologist. To make it clear, just because we tech's that gross do not have a masters as a pathologist assistant, we care just as much about the patients we serve as a PA does. Another point I would like to make is that very often we gross not by choice but because it is what our pathologists demand of us and they wouldn't put us there if we couldn't do the job. Believe me, when I say that I do want to get my masters as a PA, but I haven't been able to find a program that accommodates someone who is working full time and can not afford to quit to go back to school. I am currently finishing up my Bachelors, because I still want to pursue it. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico -- Message: 8 Date: Mon, 23 Apr 2012 16:32:34 -0700 From: Davide Costanzo pathloc...@gmail.com Subject: Re: [Histonet] Qualifications for grossing To: Glen Dawson ihcman2...@hotmail.com Cc: histonet histonet@lists.utsouthwestern.edu Message-ID: ca+f+rhoy4dypx0mpoq65rrrvldxobv_0acspzbgqrpv8ygv...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Glen, Below are the requirements for high complexity testing, as outline by CLIA. You can reference the CLIA '88 ruling, specifically look at Subpart M, Section 493.1489 The requirements are weak, to say the least. I am not alone in the opinion that just because CLIA allows it, it is not necessarily appropriate for the minimum qualified person to be grossing certain specimens. Having someone other than an M.D., or ASCP certified PA do anything larger than a skin shave is not good medicine. But, in answer to your question - yes, the government allows inadequately trained personnel to perform high complexity testing. Sec. 493.1489 Standard; Testing personnel qualifications. Each individual performing high complexity testing must-- (a) Possess a current license issued by the State in which the laboratory is located, if such licensing is required; and (b) Meet one of the following requirements: (1) Be a doctor of medicine, doctor of osteopathy, or doctor of podiatric medicine licensed to practice medicine, osteopathy, or podiatry in the State in which the laboratory is located or have earned a doctoral, master's or bachelor's degree in a chemical, physical, biological or clinical laboratory science, or medical technology from an accredited institution; (2)(i) Have earned an associate degree in a laboratory science, or medical laboratory technology from an accredited institution or-- (ii) Have education and training equivalent to that specified in paragraph (b)(2)(i) of this section that includes-- (A) At least 60 semester hours, or equivalent, from an accredited institution that, at a minimum, include either-- (1) 24 semester hours of medical laboratory technology courses; or (2) 24 semester hours of science courses that include-- (i) Six semester hours of chemistry; (ii) Six semester hours of biology; and (iii) Twelve semester hours of chemistry, biology, or medical laboratory technology in any combination; and (B) Have laboratory training that includes either of the following: (1) Completion of a clinical laboratory training program approved or accredited by the ABHES, the CAHEA, or other organization approved by HHS. (This training may be included in the 60 semester hours listed in paragraph (b)(2)(ii)(A) of this section.) (2) At least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. (3) Have previously qualified or could have qualified as a technologist under Sec. 493.1491 on or before February 28, 1992 On Mon, Apr 23, 2012 at 1:19 PM, Glen Dawson ihcman2...@hotmail.com wrote: All, Can a histotech perform GROSSING if he/she has an associate's degree in Histotechnology from an accredited institution (Argosy in MN)? Any help would be appreciated. Thank-you, Glen Dawson BS, HT(ASCP) QIHC Histology Technical Specialist Mercy Health System Janesville, WI
[Histonet] RE: Histonet Digest, Vol 101, Issue 32
Davide and Rene, you have very valid points and I do not necessarily disagree with you. But the reality is that it is an accepted CAP/CLIA allowed practise and will continue. You both have the right to voice your opinions on the issue, but perhaps histonet which is made up mostly of techs, many of whom gross (not by choice) is not the best place to do it without causing a lot of controversy. You need to take your concerns where they might make a difference, to CAP or CLIA. If you believe in it strongly enough you will try and do something about it. Just know that those of us who do gross, do everything within our power to do the job safely for those patients we serve. Respectfully Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico -- Message: 14 Date: Wed, 25 Apr 2012 09:34:29 -0700 From: Davide Costanzo pathloc...@gmail.com Subject: Re: [Histonet] RE: Qualifications for grossing To: Joanne Clark jcl...@pcnm.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: ca+f+rhqo7guohqtlxta1ffd2yhda0br1hefi3rdh2woji35...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Joanna, I wanted to take an opportunity to explain my, and most of my colleagues, feelings about CLIA '88 with respect to grossing standards. But I want to start by stating that this goes both ways, I also do not feel it is appropriate for an ASCP certified PA to be performing Immunohistochemistry, or other stains in the lab. Both histotechnicians (ologists) and PA's have a very clear role in the pathology laboratory. Both have very different training programs. Both HT's and PA's should be protected by law, and rules/regulations for each should be clear. One is not better than the other, and I certainly hope you do not think I have an opinion different from that. Both are highly qualified individuals in their area of expertise. In many states, and I will use Florida as an example because that is what I am familiar with, there are clear definitions in the law as to whom can perform what tasks. In the State of Florida, a PA (regardless of training level) is not to perform frozen sections. That State only allows Pathologists and HT's to cut a frozen. This is the result of much effort put in to changing those rules by the HT's in Florida. Clearly they saw PA's as a threat to their job, and took action. Not a problem, I am happy to let them do the frozen sections. What was it about cutting a frozen section that the HT's thought a PA could not handle? I do not know, but nonetheless they reacted. Certainly PA's are heavily trained in how to cut a frozen section, and it is generally considered our responsibility in most places in the US that I have seen, and I have seen many. Rarely, outside the State of Florida, do I see PA's that do not cut frozens. Now, on to the issue of grossing techs. There are myriad reasons why I, and most of my peers, think it is not appropriate to utilize grossing techs. For starters, and to be clear, the use of such techs serves one principal purpose to the pathologist's and institutions that employ them - to save money and increase their profits. They are not employed because they represent the clear choice for the utmost in patient care, and to suggest that is not just misleading, but completely false. Grossing small specimens is never just about transferring tissue from a container to a block. Many tend to try and downplay the importance of that task, and overlook things that could be problematic without certain training/skills. And, there are many grossing techs that do larger cases, from gallbladders all the way up to mastectomies and beyond - all with no didactic education, no proficiency testing and no rotations through various types of insitutions. I have never seen a study, but perhaps someone on here has, that points out the sharp increase in error rates found when a tech is used to gross, versus a trained pathologists' assistant. There is a drastic difference. It is distinct, and a study is really not needed to see that difference. Now, to be clear again, that is not to say that every tech that grosses does a bad job. No vitriol here. It is just a fact, and a troubling one at that. Imagine the difference in quality you would see if you had me doing all your stains! I am not trained as an HT. You could argue that I could be trained, but do you really want to open that can of worms? Do you want medicine to allow for that, and risk the HT profession? Probably not, and we do not either. Do you think I would be as good as you are, given all the real education you received when getting your HT training? I don't think I would be as good as you are at doing your job. As an example to illustrate, anyone that grosses should know how to answer these very basic questions. These might help shed some light on the issue: 1. What is the most common neoplasm of the gallbladder, what does
[Histonet] RE: Histonet Digest, Vol 101, Issue 1
We don't. And CAP had never questioned it. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico -- Message: 1 Date: Mon, 2 Apr 2012 10:29:56 -0500 From: Tunde Ajibade tajib...@echd.org Subject: [Histonet] IHC QC log from the Ref Lab To: 'histonet-boun...@lists.utsouthwestern.edu' histonet-boun...@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: abf9e8674bbe5448920911351ac6d3e502181c1...@mchmail.echd.org Content-Type: text/plain; charset=us-ascii Hello Everyone, If you send IHC to a ref lab to do the technical components only and you have pathologists onsite to interpret the slides, do you need the QC log for that stain from that ref lab? If you already have both positive and negative control slides from that ref lab. Thank you. Tunde Ajibade BS, HTL(ASCP)QIHC CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 101, Issue 1 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 100, Issue 15
We also have a first generation Autostainer and I have had the same happen with my machine. I believe it is definitely a mechanical issue. The engineer that came out changed the motor driver unit and we haven't had a problem since. He also told me that it can also be the belt drive on the arm that drives the XY that could be a problem if it persists. Get another service call in and get them back out to fix the unit. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico Message: 5 Date: Mon, 12 Mar 2012 14:31:44 -0400 From: friedrich_h...@bd.com Subject: [Histonet] DAKO Autostainer Issues To: histonet@lists.utsouthwestern.edu Message-ID: of3e9d799b.1e276f03-on852579bf.0064fb8c-852579bf.0065c...@bd.com Content-Type: text/plain; charset=US-ASCII We have been having issues with our Autostainer since bringing it out of storage. All parts are in excellent shape, according to the technician that performed the PM. It worked fine for a month of so, but then began malfunctioning after only a couple of staining runs. At some point in the staining process, the machine appears to lose its XY homing and jams itself against the front right corner, dispensing buffer onto the floor of the chamber and occasionally making grinding noises. We've had a technician take a look at it, 'clean' the software, and we thought we had it resolved, but the problem appears to be back. Has anyone experienced this issue? If so, was it hardware- or software- based? Thanks in advance! - *** IMPORTANT MESSAGE FOR RECIPIENTS IN THE U.S.A.: This message may constitute an advertisement of a BD group's products or services or a solicitation of interest in them. If this is such a message and you would like to opt out of receiving future advertisements or solicitations from this BD group, please forward this e-mail to optoutbygr...@bd.com. *** This message (which includes any attachments) is intended only for the designated recipient(s). It may contain confidential or proprietary information and may be subject to the attorney-client privilege or other confidentiality protections. If you are not a designated recipient, you may not review, use, copy or distribute this message. If you received this in error, please notify the sender by reply e-mail and delete this message. Thank you. *** Corporate Headquarters Mailing Address: BD (Becton, Dickinson and Company) 1 Becton Drive Franklin Lakes, NJ 07417 U.S.A. *** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cytokeratin AE1/AE3
Hi Histonetters, I am having some issues with my Cytokeratin AE1/AE3. We use the antibody from DAKO and do HIER on it before staining. We run AE1/AE3 as a protocol on sentinel lymph nodes from breast cases that were negative on frozen section to rule out micro metastases and what my pathologists sometimes sees is staining that looks like it's running in-between the cells. It is not an indication of metastases but my pathologists want me to get rid of it. Have any of you seen anything like this? I would especially appreciate the opinion of Samuri Pathologist on this if it isn't too much trouble. Thanks all! Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico Roswell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pigment in HE slides
Hi Histonetters, I was hoping you all could help me identify a strange pigment I have occassionally seen in my HE slides. It's not a formalin pigment and looks to be in the tissue, but on top at the same time. When you focus up and down on it with the microscope its slightly refractive. It looks more to me like a precipitate really than a pigment. It doesn't happen on all slides just on the odd one which makes it easier to rule out formalin pigment. IF the pH was off on my formalin, all the blocks would have pigment in them. It almost looks to me like talcum powder. I was able to remove it by bleaching it out with potassium permanganate. Any ideas? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Breast CAP requirements
We have a tech come in on the weekends to embed so the fixation never exceeds 48 hours. We keep a log to record the fixation times of all our breast cases. For needle core biopsies if the client has written the time the specimen was taken on the requisition, we record that time otherwise it is the time we received it in the lab. With breast lumpectomies or mastectomies the fixation time starts once the specimen has been grossed and blocked, not before. With each report we have a blurb stating the fixation time of the specimen. It goes something like: 'The specimen has been fixed for a minimum of 12 hours to a maximum of 48 hours in accordance with CAP breast fixation guidelines for Her2Neu testing'. Sometimes though, courier delivery causes delays and its already been over 48 hours when we receive them in the lab. For instance a client does a breast needle core biopsy on a Friday but we do not receive the specimen until the following Monday. In these instances we record on the report that the specimen has had greater than 48 hours fixation. We do not do the Her2Neu testing in house and when we send them out for testing we have to record on the outside consultants requisition the fixation time. Sometimes its impossible not to go over the 48 hours and when it happens we just record it in the report. Joanne Clark, AAS,HT(ASCP) Histology Supervisor Pathology Consultants of New Mexico -- Message: 1 Date: Fri, 03 Feb 2012 12:51:47 -0500 From: Richard Cartun rcar...@harthosp.org Subject: Re: [Histonet] Breast IHC testing To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu,Gale Limron ga...@unionhospital.org Message-ID: 4f2bd863.7400.007...@harthosp.org Content-Type: text/plain; charset=US-ASCII Those are recommendations from the CAP. You can experiment and then validate longer fixation times. Several papers have come out over the past two years demonstrating little, if any, impact on immunoreactivity for ER, PR, and HER2 when breast tissue has been fixed longer than 72 hours. In my opinion, minimizing cold ischemic time, making sure the tissue does not dry out, and taking thin slices of tissue (2-3 mm) for fixation and processing are probably more important than time in formalin. Hopefully, ASCO and CAP will change their recommendations based on these studies. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Gale Limron ga...@unionhospital.org 2/3/2012 10:24 AM I would like to know what other hospitals are doing with breast specimens that are resected on Friday and are in formalin longer than the maximum number of hours that CAP allows for ER/PR and HER2/neu testing. We are running into this problem since we don't currently work Saturday hours. Thank you! Gale Limron CT,HT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Aspyra LIS system
Hi Histonetters, have any of you out there had any experience with the Aspyra LIS system, specifically the anatomical pathology and cytology modules? Any feedback would be appreciated. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexcio Roswell, NM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 94, Issue 7
Hi Meredith, as far as I know New Mexico follows the CAP guidelines (CLIA88 regs) for who can gross what. I am an HT with an associates and I do the grossing of both biopsies and bigger more complicated specimens. You need an exact list of what a 'non pathologist' is approved to gross in your facility. Documentation of training and how the grossing is reviewed by the lab director is also required. According to CLIA88 you need to have an associates degree to do grossing of either biopsies or bigger specimens. All grossing is now considered high complexity testing. We just had our CAP inspection and were fine with how we have it set up and documented for a non pathologist to gross. You're the new GI lab in Las Cruces correct? Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico Roswell, NM -- Message: 16 Date: Wed, 7 Sep 2011 09:14:27 -0500 From: Hale, Meredith mh...@carisls.com Subject: [Histonet] New Mexico Grossing To: Histonet@lists.utsouthwestern.edu Message-ID: 6F33D8418806044682A391273399860F09997FBA@s-irv-ex301.PathologyPartners.intranet Content-Type: text/plain; charset=us-ascii Is anyone aware of the grossing regulations in New Mexico for biopsies ?Thxs Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 93, Issue 9
We have been using recycled xylene on our VIP5 for the past 5 years and haven't had any problems. We use it for both the cleaning cycles and the xylene stations. Joanne Clark, HT Histology Supervisor PCNM -- Message: 9 Date: Mon, 8 Aug 2011 09:33:43 -0500 From: Gaiser, Marcia marcia_gai...@ssmhc.com Subject: [Histonet] re-cycled xylene in tissue processor To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 728f817c02110e498d803a7c3b0c6248068d43b...@s009-apexm06.ds.ad.ssmhc.com Content-Type: text/plain; charset=iso-8859-1 Hi, Has anyone had experience using re-cycled xylene in the Tissue-Tek VIP5 tissue processor for the clean cycle? Will use of re-cycled xylene, over time, damage the processor? Thank you, Marcia Gaiser Pathology Supervisor Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 92, Issue 33
This sounds to me like a processing issue, especially with the types of tissues you describe. Are the tissue sections you are referring to very thick? Maybe the tissue is too big for the amount of time in the different processing stations. I have also seen this happen when the tissue has been left to dry out before the clinician puts it into the formalin. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico -- Message: 2 Date: Mon, 25 Jul 2011 13:42:25 -0400 From: Carol Bryant cb...@lexclin.com Subject: [Histonet] blurry tissue To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 50DA0C6B72976B4AB3A0FCA04CC73DBF141CC36F8E@EXCHANGESB Content-Type: text/plain; charset=us-ascii I would like some thoughts on how to resolve some blurry looking tissue. We have had occasional tissue that looks blurry and not crisp for several weeks now. It is not all the cases only random tissues. The tissue is not on the same tissue processor either. We have 2 processors. The latest cases were a breast, some skins, and a prostate. I am not certain if this is happening on the tissue processor or in the stainer. It has been very humid in our lab so I have started running a dehumidifier in case there is water in the xylene. It is so hit and miss that I am puzzled. Any suggestions would be greatly appreciated. Thank you in advance for your thoughts. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraffin Wax Waste Disposal
Hi All, we had our CAP inspection yesterday and were cited for disposing of our waste wax from the processors in regular waste. In all my 20+ years of working in histology I have never disposed of the dirty wax in biohazard waste. Especially now with the newer processors that have very little carry over. I know this is probably state regulated by is anyone aware of a regulation or documentation that states what the amount of hazardous chemical in a substance must be before it is considered hazardous? And if so, does anyone know of a way to measure the amount of xylene in waste paraffin? Thanks in advance. Joanne Clark, HT Histology Supervisor PCNM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ER/PR
Hi All, I would like some input on ER/PR by IHC. We have a lot of problems with tissue detachment from the slides. We use a DAKO autostainer and do the heat retrieval in the Pascal pressure cooker. We use high pH retrieval buffer and heat to 120 degrees and hold for 1 minute, but when you look microscopically at the slides the heat has really played a number on the tissue and it looks chewed up. The slides have been in the slide dryer for at least an hour before running, sometimes longer. Any ideas how I can reduce the effects of the heat on the tissue without compromising the staining? Thanks for your help. Joanne Clark, HT Histology Supervisor PCNM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC for H. pylori
Hi All, was wondering how everyone does their IHC for H. pylori. Do you automatically run it on all biopsies of the stomach, or do you screen first with a giemsa and run IHC on the ones that are negative by giemsa? Thanks for the input! Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] p63
I need to find a source for p63 antibody. Where does everyone purchase theirs from? Joanne Clark,HT Pathology Consultants of New Mexico ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Prostate needle core protocols
Hi all, my doc's want to know what protocol others use for cutting needle core biopsies of prostate. We cut a serial ribbon pick up one for HE and the rest are kept as unstained slides, than trim in about 16-20 microns and take another serial section same as above and than repeat a third time. How do other labs cut their prostates biopsies? Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TTF-1 Antibody
Good Morning, we are using TTF-1 antibody from Cell Marque (clone 8G7G3/1 mouse monoclonal) on a DAKO autostainer with an LSAB+ platform. We do heat retrieval in the DAKO pascal pressure cooker using Cell Marques Trilogy retrieval solution. We do a lower temp for a longer period of time during the retrieval. I incubate the primary antibody for 1 hour and I still have problems getting the marker to work with any consistency. Where do the rest of you doing this marker get your antibody from and do you have problems getting it to work consistently? Any and all feedback would be appreciated, I'm getting really frustrated. Thanks Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hematology stainers
Hi Histonetters, do any of you out there stain the peripheral, aspirate and touch prep slides of bone marrows in the histo lab? If you do, do any of you use an automated stainer and if so, what kind of stainers are being used? I have quotes for a Wescor Aeorspray Stat Slide stainer, a QuickSlide Plus Automated stainer and a Hematek 2000. If anyone has had experience good or bad for any of these stainers I would love to get your feedback. Thanks, Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job in New Mexico
Pathology Consultants of New Mexico has an opportunity for a Histology Supervisor I Las Cruces, New Mexico. Duties include grossing; frozen section processing; tissue processing, embedding, microtomy, and staining; immunohistochemistry; special handling of renal, skin and lymphoma biopsies. Prioritize, direct and maintain work flow in laboratory, ensuring compliance with quality assurance and safety standards. Monitor and ensure compliance with established histology procedures and CAP inspection standards. Maintain all inspection materials, technical policies and laboratory procedure documentation. Perform equipment maintenance as needed. Maintain supply inventory. Assist with budget development and compliance, and perform cost analyses of new technologies. Requirements: Associate of Science degree in medical technology, biology, or related life science. Also requires four years of experience as a histology technician (including at least one year in a histology supervisory role), and ASCP HT certification or eligibility for ASCP HT certification in New Mexico. Please send cover letter and resume to lbra...@pcnm.com . Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico Roswell, NM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Grossing Station
Does anyone have a used grossing station sitting around in storage that they would like to get rid of? Joanne Clark, HT Histo Supervisor Pathology Consultants of New Mexico ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 83, Issue 13
We use the CBG recycling system and it does a great job. It will do alcohol, xylene and formalin; however, we do not bother with the formalin since to have to re-pH it after (too much of a hassle when formalin is so cheap to purchase). Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico Message: 5 Date: Sun, 10 Oct 2010 23:42:26 -0700 (PDT) From: Mel John del Barrio meljdelbar...@yahoo.com Subject: [Histonet] Xylol, Alcohol, Formalin Recycling To: histonet@lists.utsouthwestern.edu Message-ID: 310131.95554...@web114508.mail.gq1.yahoo.com Content-Type: text/plain; charset=utf-8 Hi, Is there any sales reps/laboratory sup's here that can give me some info about recyling solvents and formalin? Thanks MJ Image by FlamingText.com -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 83, Issue 8
Another consideration as a few have mentioned could be with your automated stainer (if you use one). We have a DAKO autostainer and we found our staining had become very sporadic. When I did a repeat run manually, everything was beautiful. We found that a pump on the stainer was going and it wasn't washing all the reagents off the slide between steps. In addition the probe needed to be replaced as it was drawing up air into the lines and the volumes it was dispensing on the slides was variable from slide to slide depending on how much air was in the probe lines at any given time. If you do use automation, when was the last time the machine had a PM? I agree with the others that tween in your wash buffer is a must. Joanne Clark, HT Pathology Consultants of New Mexico -- Message: 1 Date: Thu, 7 Oct 2010 07:32:13 -0700 From: sris...@mail.holyname.org Subject: [Histonet] problems with staining IHC To: histonet-boun...@lists.utsouthwestern.edu, Histonet@lists.utsouthwestern.edu Message-ID: of6e74eb3d.a17b453b-on852577b5.004f018d-882577b5.005f9...@holyname.org Content-Type: text/plain; charset=US-ASCII Hi All, Our pathologists have made a complain that the staining of slides have been a problem. Same antibodies which stain one day does not work the following day. This has been going on for a few months. When we repeat stain they either work or not work and we have been sending them to reference labs. Our tech support person was here and told us that the slides ( fisher plus slides) have been a problem with some of her customers. Meanwhile our surrounding labs which are using the same slides have no issues. She showed us how the reagents were not spreading properly on the slides. Is anyone confronted this issue? If so, how was it solved? Thanks in advance. Mala Srishan Supervisor, Histology Holy Name Medical Center Teaneck NJ 07666 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Query Special Stainers
Hi All, we have an old Ventana benchmark NexES 9.0 that we used to use for our IHC. We now have a different IHC platform that we use and would like to change the Ventana over to use for running our special stains. Does anyone use the Ventana for their specials and have any advice or comments about how it performs? Thanks! Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: cpt code for fused twin placentas
We charge for two if when the specimens are received there is a clip on one of the placental cords. The same for twin placenta's that aren't fused, if there is a way to distinguish between the two, we bill two. Message: 19 Date: Fri, 20 Aug 2010 08:33:31 -0400 From: Houston, Ronald ronald.hous...@nationwidechildrens.org Subject: [Histonet] cpt code for fused twin placentas To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: e02e1309b208f94c83b968e45781001a2340572...@nchexmbx01.columbuschildrens .net Content-Type: text/plain; charset=us-ascii Can you charge 88307 x2 for fused twin placentas? I'm not sure as strictly only one specimen was received, but our billing company says yes? Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidec hildrens.org www.NationwideChildrens.orghttp://www.NationwideChildrens.org One person with passion is better than forty people merely interested. ~ E.M. Forster ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 81, Issue 18
Hi Emmanuel, I am a Canadian trained and certified tech working here in the US. You will have to contact the CSMLS (Canadian Society of Medical Laboratory Sciences) to find out were to send your educational info to apply for Canadian certification. You should be able to do a google search and get their website address. You must have Canadian certification to be able to work in any lab and there is probably no point in applying for a job in Canada until you have it. Joanne Clark, HT, MLT (Cdn) Histology Supervisor Pathology Consultants of New Mexico -- Message: 2 Date: Sat, 14 Aug 2010 17:37:24 -0500 From: Emmanuel O graceofgod011...@hotmail.com Subject: [Histonet] Seeking Position in Canada To: histonet@lists.utsouthwestern.edu Message-ID: col119-w5283faf640f630f71665c8de...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 I am a US trained and ASCP certified Histologist. I am looking for job opportunity in Canada either as Histologist or as an IHC Specialist. Thanking you all in advance. Emmanuel. -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 81, Issue 21
-- What we do is color code out microtomes (red, blue, green etc.) and each station has a matching marker. The tech will put a colored stripe down the side of all the blocks that he/she cut. If we have to pull a block out of the file for recuts, we know exactly which machine it was cut on by the color of the stripe on the side of the block. No re-alignment necessary. Joanne Clark, HT Histo Supervisor Path Consultants of NM Message: 2 Date: Tue, 17 Aug 2010 14:16:26 -0500 From: Sharon.Davis-Devine sharon.davis-dev...@carle.com Subject: [Histonet] Microtome alignment To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: fe4513daaa85804d9a9fbac9c1ee7a668edbe...@exchangeccabe.carle.com Content-Type: text/plain; charset=us-ascii We are having a continuing issue of too much tissue being cut off when facing off a block for recuts. We have tried a couple of different methods for aligning our microtomes without much success. Does anyone out there have any advice on how to properly align them and what tool to use? Also, how often do you perform this re-alignment? The majority of our microtomes are older so more wear and tear and things move out of place more often. Any help or suggestions will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 80, Issue 33
We use a similar product called Dissect Aid, from Decal Corp. IHC staining has never been an issue; we never run IHC on the lymph nodes from colon cancer cases. We usually do the IHC on the tumor itself which is fixed in formalin. This kind of product is very useful in helping to find all those pesky little nodes that like to hide in the fat by turning them white. Cancer protocols require that at least 13 nodes be submitted. As histo supervisor, I also do the grossing in my facility, so I understand why your PA is interested in this kind of product (it can be difficult to come up with this magic number of 13 without it). However, if you do run IHC on the nodes from these cases, you would indeed have to revalidate your markers with this as your primary fixative or as a post fixative. Joanne Clark, HT, MLT Histology Supervisor Pathology Consultants of New Mexico Roswell, NM - Message: 2 Date: Tue, 27 Jul 2010 15:03:33 -0300 From: Hayes, Randi (HorizonNB) randi.ha...@horizonnb.ca Subject: [Histonet] Using GEWF solution and IHC staining To: histonet@lists.utsouthwestern.edu Message-ID: c2889f00e87e8d4ea5798737d8f7f362a73...@rhaex1.rha-rrs.ca Content-Type: text/plain; charset=iso-8859-1 At a recent conference, our PA learned of using GEWF (glacial acetic acid, ethanol, distilled water, 40% formaldehyde) solution as an aid for Lymph Node retrieval in Colorectal Cancer resections. Although a good idea, I'm wondering how safe it is to use when staining for IHC. Does anyone have much experience with this or know of a study (studies) that have been done to verify that the ethanol is not destroying antigen sites? We're a little concerned.. Randi Hayes, MLT Histology Supervisor / Superviseur d'Histologie Horizon Health Network / Réseau de santé Horizon (506) 860-2157 randi.ha...@horizonnb.ca http://www.me.com/mail/ www.HorizonNB.ca http://www.horizonnb.ca/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microtome Aligner
Hi All, I just want to clarify that the microtome aligner that we are having issues with is the one made by Advanced Innovations and sold by Newcomer Supply not the one made by Tech One (Shane Perkins) which Newcomer also sells. I have had no experience with the device that Shane is referring to. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] universal microtome aligner
We purchased this item from Newcomer supply and have been universally disappointed in it. The concept for the tool is good, but we have found that it will not clamp tightly onto our Leica microtomes (model RM2125). Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 72, Issue 25
-- Are you already certified to practice in Canada? If not, you will need to contact the CSMLS (Canadian Society of Medical Laboratory Sciences). They can guide you on how to go about getting Canadian certification (which you must have to practice in Canada). If you already do, I believe each province has its own society with websites that post jobs that you can check out. If you are American, you would have to contact immigration to see what paperwork is required to get some kind of working visa. Hope this helps. Joanne Clark, HT, MLT (Canadian certification) Histology Supervisor Pathology Consultants of New Mexico Roswell, NM Message: 3 Date: Fri, 20 Nov 2009 17:36:31 -0800 (PST) From: Ade Ade adead...@yahoo.com Subject: [Histonet] HISTOLOGY JOB IN CANADA To: histonet@lists.utsouthwestern.edu Message-ID: 784992.3124...@web113919.mail.gq1.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Hi, I am an histotechnologist currently practicing in the United States. I am looking for infos on how to get job as either Histotechnologist or IHC-Tech in Canada. I will be very grateful, if you guys could furnish me with all the necessary infos. Thanking you all for your usual cooperation. Ade Ade. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 72, Issue 19
Hi Shiela, I went through an OLA accreditation before I came to work here the States and it seems to me if you do all the same QC you did for CAP you will be OK. You will need to validate your instruments and all of your IHC protocols and have records to show. All of your procedures for all your antibodies will need to be documented and how you QC test them. Unless they have changed things, you can use outdated antibodies with OLA if you have a written procedure that outlines how they are used and what to do if the QC/QA guidelines are not met. You will need to have QC testing records for when a new shipment of antibody is received from the vendor before being put into use. Joanne Clark, HT, MLT(CSMLS) Histology Supervisor Pathology Consultants of New Mexico Roswell, NM Message: 9 Date: Wed, 18 Nov 2009 07:35:11 -0500 From: sheila adey sheila_a...@hotmail.com Subject: [Histonet] IHC in Ontario Canada To: histonet@lists.utsouthwestern.edu Message-ID: bay129-w14a413cd235a011d9ca31f93...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 Hello, I've recently started working back in Canada and I am looking to find other techs that are familiar with the OLA regulations for IHCs. Seems to be a little different than the CAP guidelines. Sheila Adey HT MLT _ Eligible CDN College University students can upgrade to Windows 7 before Jan 3 for only $39.99. Upgrade now! http://go.microsoft.com/?linkid=9691819 -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 72, Issue 17
Sue, TBS had a frozen section stainer on display at NSH this year. I don't have the information right here in front of me, but if you are interested I will forward it to you. Joanne Clark, HT(ASCP)MLT(CSMLS) Path Consultants of New Mexico Roswell, NM Message: 4 Date: Mon, 16 Nov 2009 11:49:45 -0500 From: Seguin, Suzanne sseg...@hrsrh.on.ca Subject: [Histonet] Re:FS stainer To: histonet@lists.utsouthwestern.edu Message-ID: b5480a2ca1e16643800e9d572fa21ff8035b4...@resexcvs1.res.neo.local Content-Type: text/plain; charset=us-ascii Hello, Does anyone know of any FS stainer other then the Shandon Linistat Linear Stainer? Thanks Sue Sudbury Regional Hospital -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Antibody Validation
-- We work out the specifics of the protocol for each new antibody with variables such as dilution, heat or enzyme retrieval, detection system and antibody incubation times based on the manufacturer's recommendations in the insert for the antibody. We do this testing on known positive tissue for that particular marker. Once we have the protocol figured out, than we run the second part of the validation using the newly developed protocol with several cases positive for that marker (usually 10 - 15) with varying degrees of positivity if possible. We also run 10 - 15 cases of known negative tissue types to make sure our protocol is not going to give us any false positivity. If we change any part of our routine IHC protocol, such as a new retrieval buffer or antibody diluent etc., than we have to revalidate each antibody we plan to use with the new reagent and record that we are getting consistent results with the product it was originally validated with. If the results are comparable, we record the data and put the new product into use. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico Message: 9 Date: Fri, 11 Sep 2009 08:20:43 -0700 (PDT) From: Akemi Allison-Tacha akemiat3...@yahoo.com Subject: [Histonet] antibody validation To: histonet histonet@lists.utsouthwestern.edu Message-ID: 332045.51983...@web31304.mail.mud.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Good Morning and Happy Friday out there in Histo-Land! I would like your assistance in an issue that I have just become aware of regarding antibody validation. I have been requested to bring on-board approximately (7) new antibodies in the immediate future, and several antibodies later. Although, many of you who know me realize I have worked in IHC RD for a well known IHC company for many years, it has been a while since I have overseen a clinical IHC lab. I would like your assistance and input on how you are validating new antibodies. Not counting Her2-neu, it has been my understanding that it is recommended to test samples with varying antigen expression from 10-15 cases. Furthermore, you should check for Sensitivity: expression range of Positive cases, low to high (10-15 cases). Specificity: (+) vs expected (-) cases/tissues (10-15 cases) and Precision (Reproducibility): It has been my practice that any changes that are made to existing protocols, such as a new detection kit or antigen retrieval methodology, also requires re-validation. I am curious how you approach validation. Also, do any of you just use a single known positive control for that antibody, and run it, and if it is positive, feel that it is satisfactory, and put it on-line for testing. Thank you in advance for your input, and have a great weekend! Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-ta...@apmglab.com (P) E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] QIHC textbooks
Is anyone in histoland willing to sell, or know where I can find a copy of Antigen Retrieval Techniques: Immunohistochemistry Molecular Morphology by Shi, S., GU, J., and Taylor, C.R (2000). This is one of the recommended texts for the QIHC exam and it is no longer in print. I have been unable to get a copy through Amazon. Any ideas or suggestions would be greatly appreciated. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico Roswell, NM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: CAP
-- Message: 6 Date: Mon, 10 Aug 2009 09:05:44 -0500 From: Vacca Jessica jessica.va...@hcahealthcare.com Subject: [Histonet] CAP ? To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Message-ID: 938d716cd445614abbb817517557b6f4c8476...@nadcwpmsgcms09.hca.corpad.net Content-Type: text/plain; charset=iso-8859-1 How does one gather this information in the lab? What are your steps? GEN.70550 Is there documentation that each of the chemicals in the laboratory has been evaluated for carcinogenic potential, reproductive toxicity, and acute toxicity; and does the policies and procedure manual define specific handling requirements for these chemicals - Thanks Jessica Vacca Histology Supervisor 119 Oakfield Dr Brandon Fl 33511 (813) 571-5193 (813) 571-5169 FAX We take this one step further than Mr. McNemar. We take the info from the MSDS and have prepared a chart of our toxic chemicals that is kept in the documentation of our Lab Safety. It lists for the chemicals involved the specific toxic dangers and any special handling. Joanne Clark, HT Histology Supervisor Pathology Consultants of NM Roswell, NM -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] DAKO Liquid DAB
DAKO is discontinuing its product 'Liquid DAB' and wants its clients to use the Liquid DAB+ in its place. I have tried this product before and feel it gives too strong a signal. To use it I will have to re-do all my protocols and hence revalidate all my IHC. Can anyone suggest another supplier that has a liquid DAB that is similar in strength to the DAKO product? Thanks for you input. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: log book
-Original Message- -Original Message- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Hi Kathy, we are a small private lab who has the contract with the local hospital. What we have is a log book in the O.R. where the nurses have to enter the specimens for pick up; our courier than signs off on the log in the O.R. that he/she has picked up the specimen. Each specimen is checked against the log before being transported to the lab. That way, if a specimen is missing, it can be taken up with the O.R. staff immediately. Hope that is helpful to you. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico Message: 6 Date: Sun, 01 Mar 2009 17:15:47 -0800 From: Kathy Gorham gorh...@verizon.net Subject: [Histonet] log book To: histonet@lists.utsouthwestern.edu Message-ID: 27e50df4f8434674b769ee3c1a469...@kathy83b707eca Content-Type: text/plain; charset=iso-8859-1 Good Monday Morning, We had a serious incident Friday with O.R. My aide went down to get the specimens from O.R. about 9am. (which were left overs from the night before). She did not stamp in the specimens before she left. When I had time to stamp them in and record them in the log book I discovered that the colon was not there. Two other specimens from that patient where in the bag but no colon. So I went down to O.R. to see where it was. Of course no one knows what happened to the colon. The doctors are furious by all means. Now the O.R. thinks the path lab screwed up. So my questions is how do others log in the specimens as they come into the lab. We have 2 couriers that brings specimens when we are not in the lab from other hospitals. How do you make sure that whom ever brings the specimens actually brings the ones they say they do? Do you have a log book that every specimen that is brought into the lab is written down by the person who brings it in? Right now we have a log book but it is written in as we are accessing the specimens. So the specimens may have been there overnight. We are a very small lab and we do almost everything by hand including writing in the log book. Someday we want to be able to scan by bar codes but right now we can not do that. Thanks for any help you can give me. Kathy Gorham, H.T. -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Glass coverslippers
Good Morning All, Can anyone recommend a good glass coverslipper? I have quotes on Leica CV5030, Surgipath and the older Tissue Tek G1. What has everyone's experienced been? Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico Roswell, NM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
