[Histonet] CRYOSTAT RECOMMENDATIONSRE: Histonet Digest, Vol 125, Issue 24
Good ;Morning, We have the Leica 1950 in our Laboratory and are extremely pleased with the insturment. Karen J Kay, MLT Supervisor - Histopathology and Cytology Laboratory Chinook Regional Hospital - South Zone West - Alberta Health Services Lethbridge, Alberta, Canada karen@albertahealthservices.ca -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: April 21, 2014 11:02 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 125, Issue 24 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Xylene substitute for processing GI biopsies (Joe W. Walker, Jr.) 2. Alport's testing (Houston, Ronald) 3. Re: Xylene substitute for processing GI biopsies (Rene J Buesa) 4. Recommendations cryostat (Pathology-Histology Sr. Supervisor) 5. RE: Paraffin (Cameron, Elizabeth) 6. COM 30450 (Piche, Jessica) 7. RE: Recommendations cryostat (WILLIAM DESALVO) 8. RE: Recommendations cryostat (Bea DeBrosse-Serra) 9. anti mouse iNOS (Anna Hughes) 10. Re: RE: Recommendations cryostat (Kim Tournear) -- Message: 1 Date: Sun, 20 Apr 2014 23:36:05 + From: Joe W. Walker, Jr. joewal...@rrmc.org Subject: RE: [Histonet] Xylene substitute for processing GI biopsies To: Rene J Buesa rjbu...@yahoo.com, Lori Gemeinhardt loreli...@mac.com,histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 3c2378778400ad448ada6fd6bdb7182a0...@rrmbx03.rrmc.local Content-Type: text/plain; charset=iso-8859-1 Are you required to revalidate all of your IHC antibodies once you switch to a xylene sub? We had utilized a xylene free process in our Peloris, which utilized alcohol but we ran into issue with FISH for Her2 and some of our other predictive markers and abandoned the alcohol. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewal...@rrmc.orgwww.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, April 18, 2014 11:15 AM To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene substitute for processing GI biopsies Hi Lori: Instead of xylene you can use isopropyl (also known as 2-propanol and propyl alcohol) and after sectioning you can use dishwasher soap before going directly to the staining procedure. After staining you need nothing but an oven to dry out the stained sections and go diractly to coverslipping. If you want details on the above please go to: http://www.histosearch.com/rene.html and read my articles on the subject, including processing protocols. If you follow the instructions, you will have a totally xylene free lab. Ren? J. On Thursday, April 17, 2014 8:19 PM, Lori Gemeinhardt loreli...@mac.com wrote: Hi there! I have spent a lot of time reading the archives, but still need some specific and current input/advice. I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with a xylene substitute. I need insight on protocols, brands, effects on staining. The good, the bad, the ugly. I have only used xylene in my many years of experience! This is completely new (to me), and rather overwhelming. It would be ideal to use the same substitute in the staining process, so any consideration with that is also appreciated. I understand this has been discussed, at length in the past, and I apologize I am just hoping to zero in on the best case scenario as a starting point. Thanks, in advance, for your help! Lori ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from
[Histonet] RE: Histonet Digest, Vol 107, Issue 14- CLEANING MOLDS
Good Morning Bill, We clean our molds once a week via the purge cycle in our tissue processor. They come out very clean and ready for a release spray after which they are stacked and placed back into the embedding unit. The molds are used for the week. We alternate the cleaning cycles amongst the embedding centres so that all molds are not being cleaned at the same time. This system has worked well for us and has eliminated much of the manual aspect of performing this task. It also greatly lessens the possibility of any small fragments of tissue that could be left behind if the tray is removed before the block has had long eough to totally cool. Karen J Kay, MLT Supervisor - Histopathology and Cytology Laboratory Chinook Regional Hospital -South Zone West - Alberta Health Services 960 - 19 Street South Lethbridge, Alberta - Canada T1J 1W5 Phone: (403) 388-6061- Fax: (403) 388-6067 karen@albertahealthservices.ca Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Monday, October 08, 2012 2:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Metal molds OK folks, I know I should be smarter than this and I haven't seen discussion on it lately Are people cleaning their metal embedding molds after evey embedding session? If not, how often do you clean them? Do you clean them at all? If you clean them, how do you do it? Thanks Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RECYCLERS - RE: Histonet Digest, Vol 94, Issue 7
Good Morning, We use the CBG recycler and are very happy with both the performance of the instrument and support provided by the company. Karen J Kay, MLT Supervisor - Histopathology and Cytology Laboratory Chinook Regional Hospital South Zone West - Alberta Health Services Lethbridge, Alberta,CANADA karen@albertahealthservices.ca Message: 5 Date: Tue, 06 Sep 2011 12:38:36 -0700 From: Behnaz Sohrab sohra...@ah.org Subject: [Histonet] Recycler To: histonet@lists.utsouthwestern.edu Message-ID: 4e66144c.4347.005...@ah.org Content-Type: text/plain; charset=US-ASCII We are in the market to buy recycling system, any recommendation between CBG or B/R system ?? Any suggestions is deeply appreciated. Thanks Behnaz Sohrab,Manager Anatomic Pathology This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RADIOACTIVE SEEDS IN PROSTATE TISSUE
Good Afternoon Everyone, Does anyone have any information on the safety aspects of handling prostate tissue that has had radioactive seeds implanted once it is submitted for pathological examination. I am asking about all phases of the examination from gross room, embedding, microtomy, staining.I have done a search on the subject via the Archives and have only seen one reference to this issue which was a similar question posed by Peggy Wenk in 2009. Thanks very much Karen Karen J Kay, MLT Supervisor - Histopathology and Cytology Laboratory Chinook Regional Hospital South Zone West - Alberta Health Services 960 - 19 Street South Lethbridge, Alberta T1J 1W5 Phone: (403) 388-6061 Fax: (403) 388-6067 karen@albertahealthservices.ca This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ARTISAN STAINER - DAKO - WASTE DISPOSAL
Hello, A question for Dako's Artisan Stainer users as to what you are doing with the Waste disposal and in particular the Trace Metals waste. Are you neutralizing it and if so, could you provide proportions of waste to 0.85% NaCl? We have recently started using the instrument and are trying to develop the waste handling processes from the perpectives of safety of our staff and meeting our regulatory guidelines. Thank you Karen J Kay, MLT Supervisor - Histopathology and Cytology Laboratory Chinook Regional Hospital South Zone West - Alberta Health Services 960 - 19 Street South Lethbridge, Alberta, Canada T1J 1W5 Phone: (403) 388-6061 Fax: (403) 388-6067 karen@albertahealthservices.ca This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PARAPLAST QUALITY
Good Morning, Our Paraplast has also been produced by McCormick and to this point have not seen any issues. However it is my understanding that Leica(Surgipath) has taken over as supplier and producer of the Paraplast (trademark). This information was relayed to us by our Surgipath representative quite a few months ago. This past week, I received a phone call from a different Lab supplier indicating to me that there have been issues cropping up with the Paraplast from the new manufacturer. I did do a search on the Histonet and have not found messages to that effect. It would be interesting and proactive to find out if there indeed have been issues cropping up recently with the Paraplast. Thanks Karen J Kay, MLT Supervisor - Histopathology and Cytology Laboratory Chinook Regional Hospital-South Zone West - Alberta Health Services Lethbridge, Alberta, Canada karen@albertahealthservices.ca -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: June 4, 2010 11:23 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 79, Issue 4 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. outside review. (Tench, Bill) 2. Laryngotracheitis (Charles, Roger) 3. Paraplast (Rebecca Johnson) 4. RE: Paraplast (Baez, Janet) 5. Lab Manager Job Opportunity in San Francisco (adam.t.anth...@kp.org) 6. coverslippers (Gale Limron) 7. RE: coverslippers (Horn, Hazel V) 8. INI-1 (Horn, Hazel V) 9. Re: Paraplast (dkb...@chs.net) 10. AW: INI-1 (Kappeler, Andreas (PATHOLOGY)) 11. RE: INI-1 (McMahon, Loralee A) 12. Unsubscribe. (McCormick, James) 13. HE Stainer Validations (Santiago, Albert) 14. Re: Unsubscribe. (Emily Sours) -- Message: 1 Date: Thu, 3 Jun 2010 10:49:45 -0700 From: Tench, Bill bill.te...@pph.org Subject: [Histonet] outside review. To: histonet@lists.utsouthwestern.edu Message-ID: 2820431bf953bb4da3e9e1a5882265fd02863...@mail1.pph.local Content-Type: text/plain; charset=us-ascii I thought I sent this yesterday, but it didn't address properly. I guess things are different in my part of the world in terms of the frequency of this activity compared with others. Please see the rest. The policy/procedure requiring review of outside slides before definitive treatment (including surgery, radiation therapy, or chemotherapy) in another institution is a fairly common but frequently unenforced policy in many hospitals other than academic institutions. The policy may reside in the Pathology Dept, Surgery Dept, or more frequently (and safely) as a standard Hospital policy. Typically, enforceability is improved if the policy is a Hospital policy. There is an ever growing body of evidence that this policy benefits the patient, the hospital, the clinician, and the pathologist. Compliance is always an issue, especially outside of academic centers, who, out of a belief that the community pathologists are prone to mistakes that academic centers never make, insist on review before treatment. Everyone's benefit is always a nice carrot, but unfortunately, without a stick, compliance may be a challenge. For the surgeons, at least, the risk of cancellation of the case if a TIMELY request for outside review has not been made serves as a useful stick, the application of which should be applied judiciously (and perhaps with a flack jacket). Radiation and Chemo cases require either: 1) cooperation of these departments or 2) external review of all cases treated in those departments to determine whether or not case review has occurred. The goal is to, as best as possible, confirm the diagnosis as well as other aspects that may have an impact on the appropriate therapy, and to do so without significant delay in the patient's care. Anything that makes the process easier is a good idea. In my system, the responsibility of the treating physician is to fax to the pathology dept a copy of the report when they have decided to proceed with additional treatment (surgery, rads, etc), and the pathology dept does all the work associated with requesting the slides and insuring a timely review. We advise (and continuously train) the outside office staff that they must provide us this information ASAP because some outside institutions are woefully slow in sending
[Histonet] DAB WASTE
Hello Jennifer, We are a hospital site and operate 2 Dako Autostainers. We collect our DAB waste into empty plastic carboys from Coulter Diluent used in our Hematology Department. These containers are encased within a cardboard cover. They are very convenient as we can place the hose directly into the waste container from the Dako itself. Our volume is such that we discard the containers on a weekly basis. Hope this information is useful to you Karen K Histopathology Dept Lethbridge Regional Hospital Lethbridge,Alberta, CANADA -- Message: 6 Date: Wed, 13 May 2009 05:51:33 -0700 From: Jennifer Campbell jcampb...@vdxpathology.com Subject: [Histonet] Question about DAB waste To: histonet@lists.utsouthwestern.edu Message-ID: 5658cbdb9eae6545abe50d2563d81bf821c...@vdxserver01.vdxpathology.local Content-Type: text/plain; charset=US-ASCII We just purchased a Dako Autostainer and are in the process of figuring out how to get rid of the haz waste that we will be generating. The machine separates the haz from non-haz, thankfully. I've been communicating with our waste facility and have been having some difficulty. They want to know a lot of specifics like, how much waste we will be brining to them at a time and how often, what sort of containers we will be bringing it in etc. Do you let the haz waste carboy get to a certain level before emptying it? Is it ok to pour this waste into plastic containers of some sort (we were going to use empty distilled water bottles) to transport them to the facility? I would appreciate any insight. Thank you. Jennifer C. This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
