Position open at Shands Hospital in Gainesville, Fl
Histotechnologist, Full-time, day shift
http://Shands.org
Kendall A. Neely
Histology Technical Specialist
Shands Rocky Point Laboratories
(352) 265-0111, x72113
>>> 8/11/2010 1:02 PM >>>
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Today's Topics:
1. histological process video (Liette Tougas)
2. brain microvessel staining (Niloofar Rezvani)
3. Tissue Tek SCA Coverslipper (Mighnon Lashus)
4. Paraffin (zodia...@comcast.net)
5. Slide label printers (Hannen, Valerie)
--
Message: 1
Date: Tue, 10 Aug 2010 13:51:19 -0400
From: Liette Tougas
Subject: [Histonet] histological process video
To: "histonet@lists.utsouthwestern.edu"
Message-ID:
<7618bc6d53f39149b7b57615c218aadf114f5f3...@exchange.ad.dawsoncollege.qc.ca>
Content-Type: text/plain; charset="iso-8859-1"
Good day,
I am looking for a video/CD presentation of the whole histological process for
the Histotechniques course. Thank you very much in advance for a prompt reply,
Liette Tougas
Faculty, Medical Laboratory Technology Program
Dawson College, Montreal, Canada
--
Message: 2
Date: Tue, 10 Aug 2010 14:16:37 -0400
From: "Niloofar Rezvani"
Subject: [Histonet] brain microvessel staining
To:
Message-ID: <4c615f64.b59e.002...@usuhs.mil>
Content-Type: text/plain; charset=US-ASCII
Hi,
I appreciate if someone can answer my question. I am going to stain rats' brain
microvessels with ER(estrogen receptor) antibody. We know how we can isolate
the vessels but this is the first time to stain them.
I have the microvessels in PBS 1X and do you know how I can fix them with
paraformaldehyde and mount them on the gel-subbed slides? I did 2 ways: 1-
mount the fresh microvessels on the slide and wait overnight to get dried and
then fix them in 2% PFA for 30 min and after that washing steps and the rest of
IHC protocol or 2- fix the vessels with 2% PFA in the tube for 30 min and then
mount them on the slides and wait overnight to get dried.
Please advise me with any idea,
thanks so much,
Dr. Niloofar Rezvani,Ph.D,PharmD
Postdoctoral Fellow
Graduate School of Nursing
Uniformed Services University
Cell:410.615.9078
Fax:301.295.1707
Email: niloofar.rezv...@usuhs.mil
Classification: UNCLASSIFIED
Caveats: None
Classification: UNCLASSIFIED
Caveats: None
--
Message: 3
Date: Tue, 10 Aug 2010 15:38:46 -0500
From: Mighnon Lashus
Subject: [Histonet] Tissue Tek SCA Coverslipper
To: "histonet@lists.utsouthwestern.edu"
Message-ID:
<197cd0b02a81f94994a285c59c8ae05c05d29b9...@pgnexchange.pathgroup.com>
Content-Type: text/plain; charset="us-ascii"
I am having a problem with my tape coverslipper. The coverslipper is not
positioning the tape properly; when the slides are coverslipped, they have a
1mm overhang on the right side of the slide, would anyone know how to adjust
this?
Thanks,
Mighnon Lashus, HT (ASCP)
PathGroup Lab
4071 S. Access Road, Suite 107
Chattanooga, TN 37406
423-493-0207
423-493-0208 fax
mlas...@pathgroup.com
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--
Message: 4
Date: Wed, 11 Aug 2010 12:46:46 + (UTC)
From: zodia...@comcast.net
Subject: [Histonet] Paraffin
To: histonet@lists.utsouthwestern.edu
Message-ID:
<1464661975.1354742.1281530806687.javamail.r...@sz0062a.westchester.pa.mail.comcast.net>
Content-Type: text/plain; charset=utf-8
Hello,
Just wanted to know if anyone has tried Henry's Wonder Wax Xtreme Paraffin
w/DMSO
made by Mercedes Medical. What were your expirences with it? Is it comprable to
McCormick's Paraplast Xtra?
Thanks in advance,
Jenny
--
Message: 5
Date: Wed, 11 Aug 2010 09:54:39 -0400
From: "Hannen, Valerie"
Subject: [Histonet] Slide label printers
To:
Message-ID:
<5680da93771f0c48954cc8d38425e72401ab3...@ismail.parrishmed.local>
Content-Typ