Re: [Histonet] Endothelial histochemistry
Hi Ali, Although I do not ever perform endothelial immunohistochemistry in our laboratory, I know it is common to use an antibody against CD31 to selectively stain endothelial cells and this may work well in pituitary tissue. Hope that helps, Kristen Ali Nasr Esfahani alina...@student.liu.se 10/22/10 5:31 AM Hi, I am just new in this group, so I hope this is the way I can get help. I am doing histochemistry on human hypothalamus and I need to stain endothelial cells. I tried UEA-I (lectin) and I got microvessels stained as the whole and like a line. But I would like to have an image of the endothelial cells of even larger vessels, the way that I can show cell by cell in a circular shape of a vessel. Could you help me and tell me what antibody or lectin helps me best. Regards Ali ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Questions
Shirley and Amy and others responding to this thread: thanks for your posts. I am not in the histotechnology field - I'm actually a graduate student at Loyola who does her own processing, cutting, and staining of bone tissue - so I really appreciate every so-called dumb question, no matter how simple, since I have never been formally trained in the field. I assumed this was a great forum to safely ask those kinds of questions to advance my graduate student research. I'm learning new details about histology every day just by reading posts by helpful people like you. Thanks have a great week, Kristen Shirley A. Powell powell...@mercer.edu 09/20/10 10:35 AM Amy, You do not need to apologize for asking a question to which you did not know the answer. This is an educational avenue, for histology, and there is no such thing as a stupid question if you need answers to solve a problem. Those of us who teach know questions are important, even if you think you know the answer but not exactly sure, or in your case you knew but needed documented verification from others in the field. I hope your fellow workers and supervisors got the message and please feel free to ask. There are those in the field who feel this is a social network for experts and that is okay too, but the real reason NSH and histosearch was started was to expand knowledge of the histology community and to improve our profession. Remembering when histology was in the basement and no one knew we were there, it makes me proud of the progress we have made in the 48 years I have been in the field. Keep asking and share what you know, no need for apologies. Shirley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Monday, September 20, 2010 11:19 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Questions I originally asked my questions because I *knew* it was being done incorrectly and no one @ my workplace believed me when I tried to show them the way I was taught/trained-as stated in my original post. Regardless, it never occurred to me that my questions were something that would be met with oh no or oh my gosh - I feel as though I should apologize for my stupid question. However, thank you, to those who responded with your procedures. I'm making a great case based on what we know! Have a good week! Amy R. Senn Holy Spirit Health System 503 N. 21st Street Camp Hill, PA 17011 Phone: 717-763-2124 Fax: 717-763-2947 www.hsh.org Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Trouble shooting decalcified bone sections (paraffin embedded)
I section EDTA-decalcified mouse tibias frequently, and I find it helps if the paraffin is very very cold during sectioning - if the tissue starts shredding again, I press a large piece of ice to the block for a minute to cool it down without having to remove the block from the microtome. It seems to work well for me, although it may not be the most technically sound way to get the job done. I can usually get a good ribbon of sections this way when I'm having difficulty with a particular sample. I have to use an old microtome and blades because I'm a student at a research institution, without the help of professional histotechs. Our tibias are decalcified in 10% EDTA for 7 days at 4 degrees with agitation with frequent solution changes. Kristen CHEN, YIJING ych...@kent.edu 09/12/10 12:50 AM Hi, We are having difficulty sectioning paraffin-embedded decalcified adult mouse autopods (paws). The tissues shatter as soon as they hit the blade when sectioned. The autopods were soaked in CalEX for 4 days at room temp and felt extremely soft before embedding, suggesting effective decalcification. We use the Sturkey EXTREMUS low profile disposable knives on our microtome. These knives are said to be coated with the hardest nitride coating available. Any suggestions will be greatly appreciated. Sincerely, Yijing ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Rat tendon processing
Hi, I am processing rat infraspinatus tendons for Masson Trichrome staining. I've had trouble with routine processing protocols and read that adding 4% phenol to the 95% alcohol solutions may help soften the tissue to make it easier to cut. Has anyone ever tried this, or have other suggestions for preventing the tendon from getting so brittle it falls out of the paraffin? Also, should I be extending the time that the tendon spends in each alcohol station or shortening it? I am processing manually since we do not have access to an automatic processor. Thanks, Kristen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
