Re: [Histonet] CAP Requirements for Scales and Balances

[Laurie Redmond via Histonet]

Yes, there are CAP requirements, and I believe they are in either the General 
checklist or the All Common checklist.  I think the requirement is for 
calibration once a year. 

Laurie Redmond


-Original Message-
From: Piche, Jessica via Histonet 
To: histonet@lists.utsouthwestern.edu 
Sent: Wed, Jun 19, 2019 11:15 am
Subject: [Histonet] CAP Requirements for Scales and Balances

Good Afternoon All,

Does anyone know if there are stated CAP requirements in regards to the 
calibration of scales and balances, and the intervals at which they should be 
calibrated?

Thanks in advance and have a good day!

Jessica Piche, HT (ASCP)
Waterbury Hospital
Waterbury, CT
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[Histonet] Alopecia

[Laurie Redmond via Histonet]

Does anyone have an Alopecia policy that they would be willing to share?  One 
of our pathologists wants us to exhaust the block/30 slides when cutting these 
cases.  Is this normal practice?

Thanks,
Laurie Redmond
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Re: [Histonet] Bleach Regs

[Laurie Redmond via Histonet]

We don't store anything under the sinks in our lab per CAP regulations.  I do 
not understand why, nor do I have the specific checklist question at my 
fingertips, but we always make sure everything is removed from under the sinks 
at inspection time.

Laurie Redmond


-Original Message-
From: Maryann Deathridge via Histonet 
To: histonet@lists.utsouthwestern.edu 
Sent: Thu, Jan 31, 2019 7:18 am
Subject: [Histonet] Bleach Regs

Hello Histonetters!
Has anyone heard of a regulation by CAP, CLIA or OSHA regarding commercial 
bleach stored under the laboratory sink.  My lab routinely has a gallon 
container of dated/ bio-labeled commercial bleach stored under the sink for 
cleaning purposes. Daily use to clean special stain coplin jars,etc..  I had a 
suspicious med tech tell my pathologist that is was against regulations to have 
it in the lab anytime !
HUH
Responses welcomed.
Have an awesome Thursday!



Maryann  Deathridge, Lab Manager
Pathology Assoc. of  St. Thomas
4220 Harding  Pike
Bldg. SE,  Suite 504
Nashville, TN  37205
madeathri...@pastnashville.com

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[Histonet] PAS Stain

[Laurie Redmond via Histonet]

Does anyone else out there use warm water to rinse the PAS slides after the 
Schiff's reagent?  Can anyone comment on whether using warm water vs. cold 
water would have any affect on the stain?
Laurie Redmond HT (ASCP)
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Re: [Histonet] TMA slides for IHC validation

[Laurie Redmond via Histonet]

I think they usually include negative tissue in the TMA's.  Check with Biomax 
on your specific slides.

Laurie Redmond


-Original Message-
From: Dessoye, Michael via Histonet 
To: histonet@lists.utsouthwestern.edu 
Sent: Mon, Oct 15, 2018 10:34 am
Subject: [Histonet] TMA slides for IHC validation

I'm using TMA slides from US Biomax to assist with IHC validation.  I'm working 
on p120 on a Benchmark Ultra and BR2410 slides from Biomax.  The cases are all 
invasive lobular carcinoma, which should all stain positively, but only about 
half are staining positive.  I sent an identical slide to our reference lab and 
the staining matches my own.

Since it's the first time I've worked with TMA slides, am I missing something 
obvious?  Are the tissue cores generally prepared in the same fashion?  Any 
reasons why I'm seeing inconsistent staining of tissue that should all be 
positive?  All regular cases pulled of invasive lobular carcinoma are staining 
appropriately, just something unusual with these TMA slides.

Any advice is appreciated!  Thanks!

Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor | 
Commonwealth Health Laboratory Services | 
mjdess...@commonwealthhealth.net | 575 
N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484

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Re: [Histonet] VIP issue

[Laurie Redmond via Histonet]

Either: 1) the ShellSol was over-used and could no longer clean/dissolve the 
paraffin so the remaining paraffin ended up in the alcohol  2) There was water 
in the cleaning alcohol (we always use 100% alcohol - do you always use 96%?  
3) the cleaning ShellSol and cleaning alcohols were switched 4) there was a 
machine malfunction

Good Luck!
Laurie Redmond




-Original Message-
From: Gudrun Lang via Histonet 
To: histonet 
Sent: Thu, Oct 11, 2018 10:58 am
Subject: [Histonet] VIP issue

Dear all!

I have a question for those, who are familiar with the VIPs from Sakura. 

Last time we changed the reagenses the cleaning-ethanol (96%) was very milky
and even was full of many small particles. It was a paraffin-soup.

What is the cause for such a case? We have been using the organic solvent
(ShellSol) for decades as xylensubstitute. 

Maybe the quality has suffered and the ability of solving paraffin has
decreased. But are there other explanations? Maybe a malfunction of the
instrument?



Thanks in advance

Gudrun Lang

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