Re: [Histonet] Leica Prisma problem

[MARR, Penelope (EAST SUSSEX HEALTHCARE NHS TRUST) via Histonet]

I suggest an extended prime to ensure no air in the line then consider if more 
drops of xylene are needed on the slides.

Hope this helps.

Penny  :)

Penny Marr
Senior BMS

C/- Histology
Conquest Hospital
St Leonards-on-Sea TN37 7RD

penny.m...@nhs.net

0300 131 4500 ext 734914
0300 131 4914

-Original Message-
From: Paula 
Sent: 22 September 2022 17:46
To: 'Rathborne, Toni' 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Leica Prisma problem

Hi Toni,

Yes, I'm sorry..it is a Sakura product. I have called tech support and they 
want to visit but hoping I can fix it myself quicker if it's a simple fix.

Thanks,
Paula



-Original Message-
From: Rathborne, Toni [mailto:toni.rathbo...@rwjbh.org]
Sent: Thursday, September 22, 2022 9:36 AM
To: 'Paula'
Subject: RE: [Histonet] Leica Prisma problem


Paula,

Isn't the Prisma a Sakura product? Have you called the vendor to ask for 
technical support over the phone? They are usually good about doing this, even 
if you don't have a service contract. You should also mention the lot number of 
the tape that you're using so they can document and track the problem if it has 
happened to other users.

Toni Rathborne
Pathology Supervisor
Robert Wood Johnson University Hospital Somerset





NOTICE: This e-mail and its attachments, if any, may contain legally privileged 
and/or confidential information protected by law. It is intended only for use 
by the named addressee(s). If you are not the intended recipient of this 
e-mail, any dissemination, distribution or copying of this e-mail and its 
attachments, if any, is strictly prohibited. If you have received this 
transmission in error, please immediately notify the sender by telephone or by 
reply e-mail, and permanently delete this e-mail and the attachments, if any, 
and destroy any printouts.

-Original Message-
From: Paula via Histonet 
Sent: Thursday, September 22, 2022 11:56 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica Prisma problem


*** This is an External Email ***

Hello everyone,



We have the Leica Prisma film coverslipper and suddenly, there are bubbles 
underneath the film. I changed out the Xylene and inserted a new film roll but 
the problem persists.  Is there anything that you recommend to solve the 
problem?



Thank you in advance,

Paula Lucas

Lab Manager

Bio-Path Medical Group

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KU82p_qNqnQ!62MqADzSvCmn3BCClnb5s2_N3knTdw5FcHUsNGAHIOWQ9bhLFblOr-VTHzGGLyzIoxodLS-sW6xxh1lBS5Plhe6FKawFf-i9BDc$






**
 **

This message may contain confidential information. If you are not the intended 
recipient please:
i) inform the sender that you have received the message in error before 
deleting it; and
ii) do not disclose, copy or distribute information in this e-mail or take any 
action in relation to its content (to do so is strictly prohibited and may be 
unlawful).
Thank you for your co-operation.

NHSmail is the secure email, collaboration and directory service available for 
all NHS staff in England. NHSmail is approved for exchanging patient data and 
other sensitive information with NHSmail and other accredited email services.

For more information and to find out how you can switch visit Joining NHSmail – 
NHSmail Support

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Cryostat

[MARR, Penelope (EAST SUSSEX HEALTHCARE NHS TRUST) via Histonet]

Hi Brittany,
Personally I do not like the HM525NX.  It is awkward and difficult to control 
with no manual advance except the control panel.  When advancing /trimming you 
are never certain how far forward the block will travel.  The UV light fitting 
in the lid always seems to get in the way of my field of view.  Ours doesn't 
have temperature control of the block holder which can make sectioning more 
difficult.

Looking at the specs of the cryostar I would actually opt for the NX70.

Kind regards,
Penny :)

Penny Marr
Senior BMS

C/- Histology
Conquest Hospital
St Leonards-on-Sea TN37 7RD

penny.m...@nhs.net

0300 131 4500 ext 734914
0300 131 4914

-Original Message-
From: Brittany Hethcox [mailto:bheth...@hcmhcares.org]
Sent: 19 May 2021 15:21
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat

Hello everyone!

I am new to the Histology world. You all may know my previous coworker,
Rhonda Ford. She officially retired and I am so happy for her! With that
being said, I am in a pickle because the cryostat we had completely broke
down. We were having issues with it during my training period, so I never
officially got to use it. We are now looking for a new cryostat for our
department. My supervisor is asking me about different models and features,
but I don't know much about any of this. So I was hoping you all could
help! Here are the details:

We are looking at two different models:
- HM525NX
- CRYOSTAR NX50

We are also getting questions about UV versus fog mist (More specifically
the "Cold D" fog mist). So my questions are:

1) Which model do you think is a better investment?
2) Is it ok to buy used?
- It's about half the price, which my supervisor really appreciates!
- A new cryostat will have to come from overseas. The seller says used ones
will probably be available in the states.
3) Which is better, UV or fog mist?
4) Are there any other features that we should aim to include in our
purchase (e.g. height adjustment, vacutome, etc.)?

Thanks so much for your help!

--
Brittany Hethcox
Histology/Pathology
765-521-1284
Henry Community Health

--
*"We Make Lasting Connections"*Henry Community Health





This message may contain confidential information. If you are not the intended 
recipient please inform the
sender that you have received the message in error before deleting it.
Please do not disclose, copy or distribute information in this e-mail or take 
any action in relation to its contents. To do so is strictly prohibited and may 
be unlawful. Thank you for your co-operation.

NHSmail is the secure email and directory service available for all NHS staff 
in England and Scotland. NHSmail is approved for exchanging patient data and 
other sensitive information with NHSmail and other accredited email services.

For more information and to find out how you can switch, 
https://portal.nhs.net/help/joiningnhsmail

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] BenchMark ultra drawers

[MARR, Penelope (EAST SUSSEX HEALTHCARE NHS TRUST) via Histonet]

Greetings!
Earlier this month we had two slide drawers replaced.  Yesterday one of them 
opened during a run and would not close again.  Fortunately we were able to 
salvage the slide that was being stained in the drawer.  A little later I was 
removing the remaining few slides from the module and noticed wisps of smoke 
coming from the drawer.  I put the module in sleep mode and the drawer still 
would not close so I turned off the power.  As soon as I did this the drawer 
closed.

I phoned Roche and at their direction I turned the module on again and the 
drawer opened again immediately.  It would not go into ready mode so we tried 
operating the compressor manually but to no avail.  The drawer then made a 
crackling noise and smoke started coming out again.

Has anybody else had a similar experience???

Penny :)

Penny Marr
Senior BMS

C/- Histology
Conquest Hospital
St Leonards-on-Sea TN37 7RD

penny.m...@nhs.net

0300 131 4500 ext 734914
0300 133 4914






This message may contain confidential information. If you are not the intended 
recipient please inform the
sender that you have received the message in error before deleting it.
Please do not disclose, copy or distribute information in this e-mail or take 
any action in relation to its contents. To do so is strictly prohibited and may 
be unlawful. Thank you for your co-operation.

NHSmail is the secure email and directory service available for all NHS staff 
in England and Scotland. NHSmail is approved for exchanging patient data and 
other sensitive information with NHSmail and other accredited email services.

For more information and to find out how you can switch, 
https://portal.nhs.net/help/joiningnhsmail

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Update info. on old topic?

[MARR, Penelope (EAST SUSSEX HEALTHCARE NHS TRUST) via Histonet]

Hi Jeanine,
- Film coverslips scratch very easily.  Any dust in the system will scratch the 
film.
- Staining is often compromised if the film coverslip needs to be removed.
- Specific storage conditions are required to validate the adhesive on the 
film.  If the storage conditions are not met the longevity of the tape is not 
guaranteed.

- Glass is better for photography
- Glass does not scratch as easily
- Less fussy with storage requirements
- Removal does not compromise staining quality.

I hope this helps.

Penny Marr
Senior BMS

C/- Histology
Conquest Hospital
St Leonards-on-Sea TN37 7RD

penny.m...@nhs.net

(01424) 758023 ext 734914
0300 133 4914


-Original Message-
From: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) [mailto:j...@cdc.gov]
Sent: 05 August 2020 13:40
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Update info. on old topic?

Good morning!

I need to provide some information to my team about film vs. glass 
coverslipping. I have a lot of positive info. regarding film but are any cons? 
And I need to come up wit the pros of glass over film. Anyone with extended 
experience have some current information for me?

Thanks very much,

Jeanine Sanders, BS, HT(ASCP), QIHCCM(ASCP)
Centers for Diseases Control and Prevention
1600 Clifton Road NE
MS H18-SB
Bldg. 18, Rm SB-114
Atlanta, GA 30329
404-639-3590







This message may contain confidential information. If you are not the intended 
recipient please inform the
sender that you have received the message in error before deleting it.
Please do not disclose, copy or distribute information in this e-mail or take 
any action in relation to its contents. To do so is strictly prohibited and may 
be unlawful. Thank you for your co-operation.

NHSmail is the secure email and directory service available for all NHS staff 
in England and Scotland. NHSmail is approved for exchanging patient data and 
other sensitive information with NHSmail and other accredited email services.

For more information and to find out how you can switch, 
https://portal.nhs.net/help/joiningnhsmail


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Detecting copper in H staining?

[MARR, Penelope (EAST SUSSEX HEALTHCARE NHS TRUST) via Histonet]

Perhaps they did an orcein stain with H counterstain?  That would make the 
copper associated protein a dark red-brown colour but with other components 
looking like an H

Penny Marr
Senior BMS

C/- Histology
Conquest Hospital
St Leonards-on-Sea TN37 7RD

penny.m...@nhs.net

(01424) 758023


-Original Message-
From: Jennifer Phinney [mailto:jh...@vet.k-state.edu]
Sent: 10 September 2019 15:57
To: HistoNet
Subject: [Histonet] Detecting copper in H staining?

Hello all,
I am in a veterinary histology lab. One of my Pathologists mentioned that at 
two of the locations he'd been at previously (Ithaca, NY and Saskatoon, Canada) 
copper deposits stained in the routine H slides. Is anyone aware of how this 
could have been achieved? I assume it would either have to be an additive in 
the eosin or perhaps something naturally occurring in the tap water rinses?


Thanks for any assistance,
Jennifer Phinney QIHC
Histology Laboratory Administrator
KSVDL





This message may contain confidential information. If you are not the intended 
recipient please inform the
sender that you have received the message in error before deleting it.
Please do not disclose, copy or distribute information in this e-mail or take 
any action in relation to its contents. To do so is strictly prohibited and may 
be unlawful. Thank you for your co-operation.

NHSmail is the secure email and directory service available for all NHS staff 
in England and Scotland. NHSmail is approved for exchanging patient data and 
other sensitive information with NHSmail and other accredited email services.

For more information and to find out how you can switch, 
https://portal.nhs.net/help/joiningnhsmail


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Victoria blue for lung tissue

[MARR, Penelope (EAST SUSSEX HEALTHCARE NHS TRUST) via Histonet]

Miller's stain is available from VWR.
I do not recommend the BSS to stain elastin - it is not strong enough and 
misses the fine fibres.

Kind regards,
Penny :)

Penny Marr
Senior BMS

C/- Histology
Conquest Hospital
St Leonards-on-Sea TN37 7RD

penny.m...@nhs.net

(01424) 758023


-Original Message-
From: Tony Henwood (SCHN) [mailto:tony.henw...@health.nsw.gov.au]
Sent: 27 July 2019 01:44
To: Bob Richmond
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Victoria blue for lung tissue

Victoria Blue is the dye used in Miller's Stain for Elastic Tissue.
It is also used in the Roche Ventana Benchmark Stainer to stain elastic Tissue:

Miller PJ (1971) An elastin stain. Med Lab Technol 28, 148-149

Karen Percival & Zaher Radi (2017) Comparison of five elastin histochemical 
stains to identify pulmonary small vasculature, Journal of Histotechnology, 
40:3, 73-78

Yufeng Yu & Clifford M. Chapman (2000) "Elastic Tissue Staining in Human Skin" 
Histologic 32(1): 12

Roten SV, Bhat S, Bhawan J. Elastic fibers in scar tissue. J Cutan Pathol 1996: 
23: 37-42.


Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA


From: Bob Richmond via Histonet 
Sent: Saturday, July 27, 2019 4:10 AM
To: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Victoria blue for lung tissue

Amy (where?) asks: >>I was asked to do Victoria Blue stain on rodent FFPE
lung tissue to exam thickness of artery. Could anybody recommend a good
vendor of this reagent kit?<<

You can get Victoria Blue R (Colour Index 44040) from Sigma-Aldrich and
several others. I couldn't find anyone who offers a kit, only the dry dye.
There are other dyes called Victoria Blue, reported to give the same
results.

You'd have to find a method for preparing it as an elastic stain, and I
couldn't find such a method either with Google or in my old books. The
requester needs to supply you with a method. I suspect the requester is
reading an old article. There are stains for elastic tissue (which is I
suppose what you want to "exam thickness of artery") that are a lot easier
to get.

Bob Richmond
Samurai Pathologist
Maryville TN
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This message is intended for the addressee named and may contain confidential 
information. If you are not the intended recipient, please delete it and notify 
the sender.

Views expressed in this message are those of the individual sender, and are not 
necessarily the views of NSW Health or any of its entities.







This message may contain confidential information. If you are not the intended 
recipient please inform the
sender that you have received the message in error before deleting it.
Please do not disclose, copy or distribute information in this e-mail or take 
any action in relation to its contents. To do so is strictly prohibited and may 
be unlawful. Thank you for your co-operation.

NHSmail is the secure email and directory service available for all NHS staff 
in England and Scotland. NHSmail is approved for exchanging patient data and 
other sensitive information with NHSmail and other accredited email services.

For more information and to find out how you can switch, 
https://portal.nhs.net/help/joiningnhsmail


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Slide Barcode Reader Fault on Ventana BenchMark Ultras

[MARR, Penelope (EAST SUSSEX HEALTHCARE NHS TRUST) via Histonet]

Greetings All!
We have 2 BenchMark Ultras that have been on-site for almost 2 years.  Since we 
transferred our routine work onto these modules we constantly get error 
messages pertaining to the slide barcode reader.  It often implies that there 
is a problem with the slide barcode.  Often, if it is transferred to another 
drawer it will read the barcode without problem and start the run.  Other times 
we give up and go home and when we return in the morning the module will read 
the barcode without issue.  Sometimes just moving the slide to the other module 
does the trick.

It is not uncommon for one run to go through without problem but when we try to 
add another it has a tantrum and won't read most of the slide barcodes.  
Changing the barcode itself often makes the situation worse.

Add in the problems with faulty dispensers and we are growing to dread having 
to use these machines.

Has anybody else had these problems?  Does anyone have any possible solutions?

Penny :)

Penny Marr
Senior BMS

Histology
Conquest Hospital
St Leonards-on-Sea TN37 7RD

penny.m...@nhs.net





This message may contain confidential information. If you are not the intended 
recipient please inform the
sender that you have received the message in error before deleting it.
Please do not disclose, copy or distribute information in this e-mail or take 
any action in relation to its contents. To do so is strictly prohibited and may 
be unlawful. Thank you for your co-operation.

NHSmail is the secure email and directory service available for all NHS staff 
in England and Scotland. NHSmail is approved for exchanging patient data and 
other sensitive information with NHSmail and other accredited email services.

For more information and to find out how you can switch, 
https://portal.nhs.net/help/joiningnhsmail

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet