[Histonet] Position open

[Martin, Gary via Histonet]

Haven't had a response to this open position ... so I'll try one more
time;

 

We have a histo tech that is retiring in December, and we need to fill
that position. We are a small lab that services a 100 bed hospital and
the surrounding physicians. We are located in California at the base of
the Sierra Foothills. If you are interested or know someone who may be
interested, contact me.
Thanks, 
Gary Martin 
 
gmartin at marshallmedical.org.
 
 

 

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[Histonet] Position

[Martin, Gary via Histonet]

We have a histo tech that is retiring in December, and we need to fill
that position. We are a small lab that services a 100 bed hospital and
the surrounding physicians. We are located in California at the base of
the Sierra Foothills. If you are interested or know someone who may be
interested, contact me.

Gary Martin 

gmar...@marshallmedical.org.

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[Histonet] GMS

[Martin, Gary via Histonet]

I am having difficulties with a stain I've done many times ...GMS. The
light green is only staining the outer edges of the specimen. Any Ideas?


Thanks 

Gary

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Re: [Histonet] Histonet Digest, Vol 152, Issue 5

[Martin, Gary via Histonet]

We are also interested in a reasonablly price and simple document control 
system. 
Thanks
Gary

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 
[mailto:histonet-requ...@lists.utsouthwestern.edu] 
Sent: Thursday, July 07, 2016 10:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 152, Issue 5

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Today's Topics:

   1. Document control system: (J B)
   2. Re: Samples not staining with Hematoxylin or primary
  (Teri Johnson)
   3. Re: Media Lab (Lester Raff MD)
   4. Re: Media Lab (J B)
   5. MITF (Baldwin, Kathy)
   6. Samples not staining with Hematoxylin or primary
  (Anna Huntley Coffey)
   7. CWD testing (Johnson, Carole)
   8. Why is Auromine heated in Histology,  but not in Microbiology
  (Eddie Martin)
   9. Re: MITF (Eddie Martin)
  10. Special Stain (Diana Martinez-Longoria)


--

Message: 1
Date: Wed, 06 Jul 2016 17:23:18 +
From: J B 
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Document control system:
Message-ID:

[Histonet] Cytology specimens

[Martin, Gary via Histonet]

I would like to poll the Histo group concerning cytology specimens. The
discussion has come up about when to discard a fluid after processing,
and fluids that have no orders. Also, because we are a contracted
service for the hospital, and are not connected to their LIS. Cytology's
that are ordered do produce a requisition that sometimes does not follow
the specimen, which leads to missed processing. How are others handling
these two situations. 

Thanks 

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[Histonet] Cryostat Chirp

[Martin, Gary via Histonet]

My Leica CM 1850 cryostat is making a chirping sound and I can't figure
out what is doing this. Has anyone experienced this sound, if what was
the resolve. 

Thanks 

Gary

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[Histonet] Knife holder

[Martin, Gary via Histonet]

I'm looking for a high profile knife holder for my Micron HM 325
microtome, does anyone know where I might get hold of this part. 

Thanks

Gary 

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[Histonet] Leica EG1160

[Martin, Gary via Histonet]

I am considering buying a refurbished Leica EG1160 and was hoping I
could get some comments for everyone on its quality. 

Thanks

Gary

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[Histonet] Release of tissue

[Martin, Gary]

Thanks to all who responded to my requested for information concerning
the release of tissue to patients.  I seems that most facilities are not
releasing tissue, especially if it has been fixed in formalin. 

Thanks,

Gary Martin

El Dorado Pathology Med. Grp.

Marshall Med. Ctr. Pathology Dept.

gmar...@marshallhospital.org mailto:gmar...@marshallhospital.org 

 

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then the intended recipient is strictly prohibited.  If you receive this
communication in error, please contact the sender and destroy all forms
of this communication.

 

 

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[Histonet] Specimen release

[Martin, Gary]

We are revisiting our policy of releasing tissue to patients. Presently
we release placentas' to patients for Religious reasons, but it seems
to be escalating to include appendix, uterus, etc. I would like to
understand what others are doing. Thanks in advance. 

Gary

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[Histonet] Telepathology

[Martin, Gary]

We are being asked to provide evaluation of EUS (Endoscopic Ultra Sound)
specimen from  a remote location. We are provided with a microscope and
a laptop, does anyone know of a simple way to capture and transmit
images back to the pathology lab? 

Thank you. 

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[Histonet] Requisitions

[Martin, Gary]

We are having a discussion as to whether the requisition needs to remain
with the specimen at the grossing stage or can it be in a separate stack
on the grossing table, while the actual specimen is left in the on deck
tray.   I believe I have seen something that speaks to this through the
CAP guidelines, but am unable to find it.  Any help would be
appreciated. 

Thanks 

Gary

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[Histonet] Sections

[Martin, Gary]

I want to thank everyone for the great responses I received to my
question about different methods of sectioning specimens.  What I heard
as a common response was to give the Pathologist what they need to do
their job, which is exactly what I had intended and will do.  

Many thanks 

 

Gary

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[Histonet] Sections

[Martin, Gary]

One of our pathologist has requested a change in the way we cut GI
specimens.  We presently put three levels on one slide. Exp.  we face
the block, cut a ribbon and take one section of that ribbon and catch on
the slide. We then progress into the block and repeat the process until
we have three levels on the slide.  The new suggestion is to simply face
the block, create a ribbon, and take three to four section of that
ribbon and that's it no more progression into the block.  The thinking
is that the Pathologist will see what they need to in those levels, and
if not they will call for deepers. I have never cut like this and just
wanted to hear some thoughts on this method. 

Thanks

Gary

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[Histonet] Job Opening

[Martin, Gary]

We are a small pathology in the Sacramento area (Placerville) looking
for a certified histotech.  This is a full time position that offers
competitive compensation and full benefits.  You can forward your
information to Gary Martin at gmar...@marshallmedical.org. 

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[Histonet] On call

[Martin, Gary]

This question may be a bit off subject Histonet, but I have not been
able to find information on this subject.  Our facility has decided to
pay our Pathologist for their on call duties.  I have been tasked with
finding what other facilities are paying for this service. Any
information would be appreciated.

Thank You 

Gary

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[Histonet] Breast processing

[Martin, Gary]

We have someone come in over the weekend and embed the tissue.  If we go over 
the time protocol for some reason, which is not often, we will evaluate the 
specimen by FISH. Once you take the bitter pill of someone coming over the 
weekend, it works very well. 
Gary


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Monday, October 08, 2012 10:02 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 107, Issue 10

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Today's Topics:

   1. RE: Breast processing times (Weems, Joyce K.)
   2. Brain - Question and advise (Histo Histo)
   3. back online, please feel free to check it out (M. Kap)
   4. Flourescent mounting medium- PLEASE HELP!!! (Candice Smoots)
   5. RE: Flourescent mounting medium- PLEASE HELP!!! (Jonathan Cremer)
   6. Re: Brain - Question and advise (Rene J Buesa)
   7. RE: Brain - Question and advise (McMahon, Loralee A)
   8. question about decalicification end-point checks
  (Thurby, Christina)
   9. Re: Brain - Question and advise (Rene J Buesa)
  10. Re: Flourescent mounting medium- PLEASE HELP!!! (Rene J Buesa)
  11. SOX-10 (Houston, Ronald)
  12. RE: SOX-10 (Della Speranza, Vinnie)
  13. Re: question about decalicification end-point checks
  (Rene J Buesa)


--

Message: 1
Date: Mon, 8 Oct 2012 00:33:13 +
From: Weems, Joyce K. joyce.we...@emoryhealthcare.org
Subject: [Histonet] RE: Breast processing times
To: Rathborne, Toni trathbo...@somerset-healthcare.com, Diana
McCaig  dmcc...@ckha.on.ca, histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID:

e3a4ebd57a691646bcced4aa5911a030667af...@e14mbx12n.enterprise.emory.net

Content-Type: text/plain; charset=iso-8859-1

We have someone from the clinical lab drain the processor, remove the blocks 
and set them aside for us to complete on Monday morning. Has worked perfectly 
for us. j





From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Rathborne, Toni 
[trathbo...@somerset-healthcare.com]
Sent: Friday, October 05, 2012 11:18 AM
To: Diana McCaig; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Breast processing times

We have someone come in on Sunday, and work with one less person on Monday.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig
Sent: Friday, October 05, 2012 11:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Breast processing times

Can you please give me your routine for processing breast cases over the 
weekend. Do you extend the time in alcohol to achieve the 48 hour maximum, call 
someone in over the weekend, or just indicate on the patient report that the 
fixation extended beyond the 48 hours limit for HER 2, 72 hours for ER and PR. 
Is the ischemic time recorded within the lab as well as on the patient report?

Thanks

Diana

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Message: 2
Date: Sun, 7 Oct 2012 22:12:28 -0700
From: Histo Histo feulgenreact...@gmail.com
Subject: [Histonet] Brain - Question and advise
To: histonet@lists.utsouthwestern.edu
Message-ID:
cac5d+km9fkjqfacye7usfaxne_q3qd4rmwr1lqteuuppcvg...@mail.gmail.com
Content-Type: text/plain; charset=ISO-8859-1

Hi all,

Our company is planning to start receiving formalin 

[Histonet] Grandfather TC Change

[Martin, Gary]

I'm interested if anyone is dealing with the expired Grandfather TC
rule.  I'm mainly interested in how you may be negotiating with your
hospital concerning the reimbursement difference. Any thoughts would be
helpful. 

 

Gary Martin

Director of Operations

El Dorado Pathology Med. Grp.

Marshall Med. Ctr. Pathology Dept.

530-626-2608

gmar...@marshallhospital.org mailto:gmar...@marshallhospital.org 

 

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communication in error, please contact the sender and destroy all forms
of this communication.

 

 

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[Histonet] Cassette Protocol

[Martin, Gary]

It seems to me that this is somewhat guided by the funds you have to
spend on a cassette labeler.  Otherwise, if you label by hand, no
problem.  Also, the ordering factor can become a bit more detailed if
you have a varied protocol. We keep it pretty simple; white being for
single levels, greens for multiple levels, and yellow's for cytology.
We do depend on our work list and the histologist skill to identify
specimens and their possible special handling (IE; mamatome breast
biopsy's = 5 levels).  

Gary Martin

El Dorado Pathology

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[Histonet] Creative Waste solutions

[Martin, Gary]

If I'm not mistaken You can find Rex's unit through Creative Waste
Solutions (1-888-795-8300), Mercedes Medical, or American Master Tech
Scientific. 

 

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[Histonet] Cassette labeler

[Martin, Gary]

We have just received a Lamb Micro Writer (model # E22.01MWI) single
shoe.  The problem is that we did not receive an operator manual.  Does
anyone know where I can get a manual? 

 

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[Histonet] RE: Histonet Digest, Vol 88, Issue 39

[Martin, Gary]

Thank to everyone who responded to my request for information concerning
peripheral smear review.  It seems that most respondents are doing this
the way we presently do.  I have to say that I agree totally with Dr.
Richmond's method.  I will be presenting the idea that these slides are
consults not just a tehc issue.  
Many thanks 
Gary (in California) 

-Original Message-
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[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsouthwestern.edu
Sent: Wednesday, March 30, 2011 10:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 88, Issue 39

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Today's Topics:

   1. RE: Peripheral smear  (wanda.sm...@hcahealthcare.com)
   2. Laura Miller is Out of  the Office.
  (laura.mil...@leica-microsystems.com)
   3. Breast Processing (daystar0...@aol.com)
   4. Whiteplains, NY: Histology Tech, IHC Techs,   Leads  Manager
  Positions (Alyssa Peterson)
   5. RE: Ventana's Basal Cell Cocktail (Sebree Linda A)
   6. Re: Peripheral smear (Robert Richmond)


--

Message: 1
Date: Wed, 30 Mar 2011 09:45:29 -0500
From: wanda.sm...@hcahealthcare.com
Subject: [Histonet] RE: Peripheral smear 
To: gmar...@marshallmedical.org, histonet@lists.utsouthwestern.edu
Message-ID:

9e2d36ce2d7cba4a94d9b22e8328a3ba13a275f...@nadcwpmsgcms03.hca.corpad.ne
t

Content-Type: text/plain; charset=us-ascii

We also transport smears from the clinical lab to our Pathologist and
transport them back.  The Hematology techs put the Pathologist's
comments into our computer system for review by the clinicians.
Wanda

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin,
Gary
Sent: Tuesday, March 29, 2011 4:36 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Peripheral smear 

Our pathologist review peripheral smears for the clinical lab, and I'm
wondering how other facilities handle the reporting.  We presently
receive the smear from a tech,  the pathologist provides a comment and
returns it to the lab. 

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Message: 2
Date: Wed, 30 Mar 2011 10:00:49 -0500
From: laura.mil...@leica-microsystems.com
Subject: [Histonet] Laura Miller is Out of  the Office.
To: histonet@lists.utsouthwestern.edu
Message-ID:

OF69753508.1B5BFDDC-ON86257863.00527906-86257863.00527906@leica-microsy
stems.com

Content-Type: text/plain; charset=US-ASCII


I will be out of the office starting  03/25/2011 and will not return
until
04/04/2011.

I am on vacation until Monday, April 4th.  Please contact David
Archambault
if you need immediate assistance.  Thanks!!!


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Message: 3
Date: Wed, 30 Mar 2011 11:56:07 -0400 (EDT)
From: daystar0...@aol.com
Subject: [Histonet] Breast Processing
To: histonet@lists.utsouthwestern.edu
Message-ID: 8cdbd0a1d7f79fe-dd4-5...@webmail-m104.sysops.aol.com
Content-Type: text/plain; charset=us-ascii




Breast Processing:  Looking to set up a breast protocol on our Sakura
VIP processor.  Looking for times/duration of specific solutions and
types of solutions used (ie. PEN-FIX

[Histonet] Peripheral smear

[Martin, Gary]

Our pathologist review peripheral smears for the clinical lab, and I'm
wondering how other facilities handle the reporting.  We presently
receive the smear from a tech,  the pathologist provides a comment and
returns it to the lab. 

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[Histonet] RE: Histonet Digest, Vol 87, Issue 4

[Martin, Gary]

We too are a small lab in California, and faced these same questions.  We 
simply document all this in the report.  For example; we know that our tissue 
comes out of formalin on the processor at 2330.  We have the surgery staff 
document the time the specimen went into formalin.  We then subtract that time 
form 2330 and have the hours in formalin.  You are correct that it took a great 
deal of effort to see that surgery complied, but after they realized we were 
relentless with phone calls, things fell into place. Oh yes! On the weekend 
someone must come in to remove the tissue from the processor. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Wednesday, February 02, 2011 10:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 87, Issue 4

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Today's Topics:

   1. Re: Off Topic: researchers very funny (Paula Pierce)
   2. Eosin stain for determining new bone old bone (Jacquitta Taylor)
   3. TTF-1 Antibody (Joanne Clark)
   4. Re: TTF-1 Antibody (bsulli...@shorememorial.org)
   5. Her2 Fixation Requirement  (Paula Lucas)
   6. Re: Her2 Fixation Requirement (bsulli...@shorememorial.org)
   7. RE: Her2 Fixation Requirement  (Weems, Joyce)


--

Message: 1
Date: Wed, 2 Feb 2011 05:49:57 -0800 (PST)
From: Paula Pierce cont...@excaliburpathology.com
Subject: Re: [Histonet] Off Topic: researchers very funny
To: Kimberly Tuttle ktut...@umm.edu,  Histonet
histonet@lists.utsouthwestern.edu
Message-ID: 217328.82615...@web1112.biz.mail.sk1.yahoo.com
Content-Type: text/plain; charset=iso-8859-1

This is hilarious. I got it on facebook yesterday and sent it to everyone.
 
Paula K. Pierce, AAS, BA, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
631 N Broadway
Moore, OK 73160
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com





From: Kimberly Tuttle ktut...@umm.edu
To: histonet@lists.utsouthwestern.edu
Sent: Tue, February 1, 2011 9:29:25 PM
Subject: [Histonet] Off Topic: researchers very funny

If this is a repost I apologize :)

http://www.youtube.com/watch?v=Fl4L4M8m4d0feature=player_embedded




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Message: 2
Date: Wed, 2 Feb 2011 09:13:40 -0600
From: Jacquitta Taylor jacqui...@earthlink.net
Subject: [Histonet] Eosin stain for determining new bone old bone
To: histonet@lists.utsouthwestern.edu
Message-ID: 4c4b1f98-d6ef-45df-a010-851cffe11...@earthlink.net
Content-Type: text/plain;   charset=us-ascii

Robin this is the protocol we use to showing new bone old bone.

Procedure for Eosin Y 0.6 % For Staining Bone Specimen
Chemicals
EosinY, Sigma E-4382,
100% Ethanol,
Phloxine B (Sigma P-4030)
Orange G(sodium salt-Sigma O-1625)
Preparation:
Stock 0.6% Eosin
Add 6g of eosin to 900ml 100% Ethanol and100 ml of H20; stir to dissolve.
Add 50 ml glacial acetic acid adjust PH to 4.6 and 5.0. The color of the 
solution will change from opaque green to clear red.
Stock 1% Phloxine B Solution:
Dissolve 1g of Phloxine B in 100 ml distilled water and filter to make a 1% 
solution.
Stock 2% Orange G Solution:
Dissolve 2 g of Orange G in 100ml distilled water and filter to make a 2% 
solution.
Working Solution Eosin 0.6%
Make a working solution by adding 6 ml of 1% Phloxine and 6 ml of 2% Orange G 
to 238 ml of 0.6% eosin.
Old bone dark orange.
New bone light orange.
Stain 15 to 30 seconds depending on what intensity you prefer.



--

Message: 3
Date: Wed, 2 Feb 2011 09:18:23 -0700
From: Joanne Clark jcl...@pcnm.com
Subject: [Histonet] TTF-1 Antibody
To: histonet@lists.utsouthwestern.edu
Message-ID: 

[Histonet] Infectious waste

[Martin, Gary]

We are wondering if paraffin block shavings are considered infectious
waste.  

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RE: [Histonet] Infectious waste

[Martin, Gary]

Vikki

Thank you for your response.  CAP is exactly why I am asking this
question.  WE do presently have procedures for the exceptions you
mentioned.  However, it came up in conversation that shavings may be
considered infectious waste.  I have yet to find anything in the CAP
information that mention this material.  I did note your mention of
block disposal, and we have that under control per CAP requirements.
It's the grey area of shaving we are questioning. 

Gary

 

 

From: Victoria Baker [mailto:bakevicto...@gmail.com] 
Sent: Tuesday, February 01, 2011 9:35 AM
To: Martin, Gary
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Infectious waste

 

Hi

 

You may receive many opinions on this --- just a heads up.  Many
clinical labs do not consider shavings or debris from cutting to be red
bag (hazardous) waste as they are no longer a 'biological hazard' due to
the processing process which incorporates heat, alcohol and a fixative
that is expected to effectively kill any infectious agents. There are
some exceptions, which you can find in the CDC or OSHA guidelines such
as dealing with tissue of brain (Jakob-creutzfeldt) lung (TB or other
air-borne pathogens).  CAP also has requirements/guidelines for handling
these cases which you will need to have documentation for the processing
procedure.  I would suggest that you refer to these guidelines and also
to your institutions regulations on what is regarded as hazardous waste.
Above and beyond anything else make sure you document and site in your
procedures manual your sources.

 

Hope this will help you.

 

Vikki

 

PS - any spelling errors I apologize for in advance ;-)

On Tue, Feb 1, 2011 at 11:45 AM, Martin, Gary
gmar...@marshallmedical.org wrote

We are wondering if paraffin block shavings are considered infectious
waste.

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[Histonet] Pap sign out

[Martin, Gary]

We have always had our Pathologist sign out all Pap results, and are now
considering having out Cytotech sign out Pap's. Is there an agency we
have to report o before we start this process. Also, how are others
choosing the 10% for review by the Pathologist? 

Thanks 

Gary

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[Histonet] JAHCO vs. CAP

[Martin, Gary]

We are having a discussion about switching our inspection process form
CAP to JAHCO. One of our questions is,  can we still participate in the
proficiency testing through CAP If anyone has done this can you explain
the process and the upside and the down side.  

Thanks 

Gary M. 

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[Histonet] Bone Marrow clot

[Martin, Gary]

We have recently been receiving bone marrow specimens where the syringe
has been heparinized before the aspiration is retrieved.  We are having
problem getting the clot sections to stay on the slide.  Could the
heparinization be causing this problem, and if so, is there a solution? 

Thank you 

Gary

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[Histonet] !0% buffered formalin corrosive?

[Martin, Gary]

I have a question around 10% buffered formalin being shipped to us.  The
shipper considers this corrosive.  I have been told that if the formalin
is shipped in 6oml biopsy bottles it does not receive that corrosive
rating when shipped.  I have also been told that if the shipment of
formalin is on a pallet and it weights 220 lbs or more, it will not
receive the corrosive rating when shipped.  Can someone bring some
clarity to the corrosive rating for me. 

Much thanks 

Gary

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RE: [Histonet] xylene substitutes

[Martin, Gary]

We use Formula 83 ... we have had no problems in all phases.
Gary

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
njoydo...@aol.com
Sent: Wednesday, May 13, 2009 7:25 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] xylene substitues

just wanting to see what everyone's favorite xylene substitute is for
clearing slides before coverslipping?? 

Gene
Cleveland Clinic
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RE: [Histonet] Leica cryostat

[Martin, Gary]

We have an 1850 and like very much, but see if you can find one with the
ultra violet light for sterilization.  It would save a lot of hassle.
Gary 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dertien,
Janet
Sent: Wednesday, May 13, 2009 1:21 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica cryostat

Hello everyone,

 

We have decided to purchase a refurbished Leica cryostat and are trying
to decide which model to choose; does anyone have experience with the
1510, the CM 1800 or the 1800? Any feedback would be appreciated.
Thanks!

 

Janet

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RE: [Histonet] Microtome Alignment

[Martin, Gary]

As an old carpenter I quickly adapted a speed square to do pretty much
the same thing as the $775.00 unit or the torpedo level.  The speed
square has the advantage of squaring the block holder to a true flat
surface (the base of the microtome) it works quite well ... it is
positive and quick.  See the Ace website below. I also use the torpedo
level as a double check. 
http://www.acehardware.com/search/index.jsp?kwCatId=kw=speed%20squareo
rigkw=speed%20squaresr=1
 Gary  (in California) 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor
Tobias
Sent: Thursday, May 07, 2009 9:21 AM
To: Jodie Robertson
Cc: histonet
Subject: Re: [Histonet] Microtome Alignment

I just went to Newcomer Supply to see what you get for $700, not much. 
Go to Ace Hardware and get each employee one of these. It will work just

as well and works on any microtome. Buy your staff lunch with what you
save.

http://www.acehardware.com/product/index.jsp?productId=1286367cp=fbc=1
pg=3kw=levellmdn=Price+Rangefbn=StorePrice%7CUnder+%2425.00fr=Store
Price%2FACE%2F%2F2500parentPage=searchsearchId=37640061873

http://www.acehardware.com/product/index.jsp?productId=1286367cp=fbc=
1pg=3kw=levellmdn=Price+Rangefbn=StorePrice%7CUnder+%2425.00fr=Stor
ePrice%2FACE%2F%2F2500parentPage=searchsearchId=3764006187
3

Victor

Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
vic...@pathology.washington.edu
206-598-2792
206-598-7659 Fax
=
Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use 
of the intended recipients. If you are not the intended recipient, or 
if the message has been addressed to you in error, do not read, 
disclose, reproduce, distribute, disseminate or otherwise use this 
transmission. Instead, please notify the sender by reply e-mail, and 
then destroy all copies of the message and any attachments.



Jodie Robertson wrote:
 Newcomer Supply.


 Jodie Robertson, HT (ASCP) QIHC
 Pathology Sciences Medical Group
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
kristen
 arvidson
 Sent: Thursday, May 07, 2009 6:24 AM
 To: histonet
 Subject: [Histonet] Microtome Alignment

 Does anyone know where I can an alignment device? 


   
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RE: [Histonet] Embedding Stamp???

[Martin, Gary]

Go to a Pipe shop and look into tobacco tampers. Very useful ... they
have a longer handle and don't seem quite as hot as the square Histo
tamper.
Gary

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yeonju
Shim
Sent: Tuesday, April 14, 2009 6:54 AM
To: histonet; histonet-ow...@lists.utsouthwestern.edu
Subject: [Histonet] Embedding Stamp???

Hi,
I am trying to order a tool that I can flatten wavy tissues down at the
bottom of the mold for embedding.
It looks like a little metal square (kind of) stamp.
Do anyone know what it's called and where I can order?
Thank you,
Judy
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RE: [Histonet] Re: BEWARE aa aa staying with a link

[Martin, Gary]

If you have clicked on the aa aa attachment and can't get out of the loop ... 
use the ALT control delete buttons pushed simultaneously and you will get to 
the task manager ... highlight the attachment and click the button that says 
end task now that should get you out of the loop. 
Gary (California) 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi 
Allison-Tacha
Sent: Wednesday, April 08, 2009 8:17 AM
To: LINDA MARGRAF; Andrea Grantham
Cc: histonet@lists.utsouthwestern.edu; MKing
Subject: [Histonet] Re: BEWARE aa aa staying with a link 

Hi,Late last night I saw the posting from  aa aa regarding staying, and there 
was a link, which I stupidly linked on to.  What a BIG MISTAKE!  It is a 
musical video.  I started listening to it and it was cute, then I tried to 
close it. It stayed on, and on, and on!  I have a Mac Book Pro Laptop, and I 
couldn't even do a force quit!  I had to remove the battery and do a start-up 
cycle.  I don't know if anyone else has had this problem, but DON'T TAKE THE 
CHANCE!

Akemi
Akemi Allison-Tacha BS, HT (ASCP) HTL

Histology Manager

Associated Pathology Medical Group Laboratories

105A Cooper Court, Los Gatos, CA 95032 

Cell: (425) 941-4287

E-Mail: akemiat3...@yahoo.com

--- On Wed, 4/8/09, Andrea Grantham algra...@email.arizona.edu wrote:

From: Andrea Grantham algra...@email.arizona.edu
Subject: Re: [Histonet] histonet off-topic comments
To: LINDA MARGRAF linda.marg...@childrens.com
Cc: histonet@lists.utsouthwestern.edu, MKing mak...@ufl.edu
Date: Wednesday, April 8, 2009, 7:18 AM

Linda,

This really is too much - now I have this thing on my computer maliciously put 
there by aa aa that I can not get to close. More than one person has to be 
removed from the list and if Bernie was removed why are there emails from him 
this morning? Histonet has helped me so much but I'm afraid this is the last 
straw. I'm going to have to unsubscribe. Sad to see such a wonderful resource 
go down the tubes because of a handful of inconsiderate bastards.

Andi Grantham


Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cell Biology and Anatomy
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edu
Tel: 520.626.4415     Fax: 520.626.2097

happy slicing and dicing and may all your stains work perfectly - Paula 
Sicurello




On Apr 7, 2009, at 9:52 AM, LINDA MARGRAF wrote:

 Dear Histonet members:
 I have removed Bernie Taupin from the list.
 Let's please get back to the topic of histology and related fields.Thanks
 Linda M
 Histonet administrator
 
 MKing mak...@ufl.edu 4/7/2009 8:43 AM 
 a motion is hereby offered to have the active members of histonet vote
 this troll off the island, or at least request that the list moderator
 do so.  this person has proven to have absolutely nothing of value to
 contribute to this group, and is only intent on disrupting it.
 
 second?
 
 -
 Message: 16
 Date: Mon, 6 Apr 2009 15:43:07 -0700 (PDT)
 From: Bernie Taupin bernietau...@ymail.com
 Subject: Re: [Histonet] Nice one Kemlo
 To: histonet@lists.utsouthwestern.edu
 Message-ID: 453473.65848...@web43516.mail.sp1.yahoo.com
 Content-Type: text/plain; charset=iso-8859-1
 
 Instead of spitting your venom try to learn
 
 Sort of like you might consider learning correct English? I mean, its
 one thing if its not your first language, but despite how many people on
 here called you kindly and helpful, I find it perplexing that you feel
 compelled to call me a piece of ignorant, and TWICE, no less.
 
 I do not speak French. This list is not in French. Ergo, my awareness
 (or lack thereof) of proper French diction has absolutely nothing at all
 to do with anything... aside form providing you a vehicle to call me
 names and lash out.
 
 If you have something to say, say it. The simple fact that you do not
 like me does not give you- or anyone else- wholesale right to insult me
 or call me names.
 
 You should be ashamed of yourself, Rene. I never expected such
 antisocial behavior from you, and frankly, when you behave so
 regrettably, I feel no compulsion to learn anything form you!
 
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 not the intended recipient, 

RE: [Histonet] owners manuals

[Martin, Gary]

I couldn't agree more Joe.  I have dealt with Mr. Royer on several occasions 
for information only and he has been very helpful.  We all entitled to blow a 
gasket every once in a while.  Be kind times are tuff.  
Gary (California for those that need to know) 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joseph Saby
Sent: Monday, March 30, 2009 3:23 PM
To: Paul Verden; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] owners manuals

I can personally attest that Ford must have been having a VERY bad day indeed.  
He has supplied excellent information to this list on many occasions to many 
people over many years, and until that listing has always been very courteous.

We've all had bad days.  I would suggest cutting him some slack.

You can never tell who you will need, or who will come to your rescue, when the 
chips are down.

Joe Saby





From: Paul Verden pver...@uropartners.com
To: histonet@lists.utsouthwestern.edu
Sent: Monday, March 30, 2009 9:49:30 AM
Subject: [Histonet] owners manuals

Personally, I would like to thank Ford the demon vendor Royer at
Minnesota Medical, Inc.for his extensive dissertation on the request for
assistance on finding manuals.  Our lab will be expanding in the near
future and will be in need of some used equipment.  It's good to know
the equipment vendors you would like to avoid.  



R. Paul Verden  



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[Histonet] Frozen section

[Martin, Gary]

We need to make a change in the way we presently account for our frozen
section while doing them.  Presently we receive the requisition with the
first specimen, then pathology is responsible to account for any
subsequent specimens.  The problem is that the subsequent specimens are
typically labeled poorly, and we are trying very hard to conform to the
CAP guidelines. So ... when the specimens are not labeled in detail, it
requires follow up calls to gain the proper information.  I would like
to know how other facilities are handling multiple frozen sections. 

Thanks 

Gary

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RE: [Histonet] Paraffin Block trimming

[Martin, Gary]

No you're not the only one ... I was wondering the same thing ... why all the 
scraping.  It seems to me that clean embedding does the trick with a few 
exceptions. 
G

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, 
Jeanine (CDC/CCID/NCZVED)
Sent: Thursday, March 19, 2009 6:58 AM
To: Paula Pierce; Histonet
Subject: RE: [Histonet] Paraffin Block triming

I was wondering if I was the only one out there that rarely has to scrape a 
block.   


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartl...@cdc.hhs.gov


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Thursday, March 19, 2009 9:57 AM
To: Histonet
Subject: [Histonet] Paraffin Block triming

I try to embed so as to have a minimal amount of paraffin to scrape from the 
blocks. ;)

But, I do scrape using the handle end of the same forceps I use to pick up the 
ribbon and tease the sections. No sharp edge. No electricity.

PKP


 




From: Peter Carroll carro...@umdnj.edu
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, March 19, 2009 8:30:34 AM
Subject: Re: [Histonet] Paraffin Block triming

 Does anybody use a paraffin block dewaxer ?

Yep, it's called my own two hands and a metal spatula, ha ha :) I find that 
it's not only very quick, but quite accurate...



Scott wrote:
 Hi,
    Does anybody use a paraffin block dewaxer ?  If so does it save any time, 
how well does it work?

 Thanks,

 Scott Hendricksen  HT (ASCP)
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RE: [Histonet] Disposal of Formaldehyde

[Martin, Gary]

First ... even if the local authorities allow this ... it doesn't make Green 
sense to do it, especially when there are other very workable alternatives.  
The path we chose was to purchase a very simple gravity feed recycler produced 
and sold by Creative Waste Solutions (888) 795-8300. Check them out.
Gary

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jessica Piche
Sent: Friday, March 13, 2009 7:11 AM
To: histonet
Subject: [Histonet] Disposal of Formaldehyde

 
Hi All, 
We have a question regarding the disposal of formaldehyde. We were told at our 
hospital that a consultant said it was okay to dump formaldehyde down the 
drain. I believe they said it was okay to dump 15 gallons or so a day! We are 
not to fond of this idea and would like to know what everyone else is doing. 
How is everyone disposing of their formaldehyde? We would be especially 
interested in what other hospitals in CT are doing. 
Thanks,
Jessica Piche-Grocki, HT(ASCP)
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RE: [Histonet] Cassette holders

[Martin, Gary]

Yes .. Hacker instruments www.hackerinstruments.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Spoon,
Victoria
Sent: Tuesday, March 03, 2009 12:17 PM
To: histo...@pathology.swmed.edu
Subject: [Histonet] Cassette holders

Does anyone know where to purchase cassette holders?  Plastic or metal
holders for cassettes - 5 across and 4- 5 deep.

Thanks,


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RE: [Histonet] breast bx processing

[Martin, Gary]

We come in and process them ! 

-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of
Margiotta, Michele
Sent: Monday, November 17, 2008 9:37 AM
To: [EMAIL PROTECTED]
Subject: [Histonet] breast bx processing

Hi All,

We are trying to figure out how to adjust our processing schedule for
breast core bxs on the weekends so that they are not sitting
in formalin for too long.  Just wondering what everyone else is doing.
Your input would be very helpful.  Thanks! 

Michele Margiotta
BMHMC
Histology Supervisor
631-654-7192




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RE: [Histonet] Presidential Voting Information

[Martin, Gary]

I absolutely agree ... THIS IS NO PLACE TO POST YOUR OPINON concerning politics 
!

-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Yaskovich, Ruth 
A (NIH/NIDCR) [E]
Sent: Monday, November 03, 2008 8:19 AM
To: [EMAIL PROTECTED]; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Presidential Voting Infomation

I don't think the Histonet is appropriate for this type of Posting!
Ruth Yaskovich
N.I.H.

-Original Message-
From: Justin Thomas [mailto:[EMAIL PROTECTED] 
Sent: Monday, November 03, 2008 11:11 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Presidential Voting Infomation

Barack Obama will raise taxes on hardworking Americans to give a government 
handout to the 40% of Americans who pay no income taxes.
John McCain and Sarah Palin have an economic plan that celebrates the American 
dream of opportunity, not government giveaways. In this country, we believe in 
spreading opportunity, for those who need jobs and those who create them. While 
Barack Obama is ready to spread the wealth around, John McCain has a plan to 
get our economy moving so everyone has access to good jobs, a quality education 
and the opportunity to succeed.
 
The next President won't have time to get used to the office. America faces 
many challenges here at home, and many enemies abroad in this dangerous world. 
We cannot spend the next four years as we have spent much of the last eight: 
hoping for our luck to change at home and abroad. We need a new direction, and 
John McCain and Sarah Palin will fight for it. 
 
Time and time again this team of mavericks has stood up, taken on tough issues 
and delivered. They're the real deal. They have a clear record that can deliver 
results, not just rhetoric that delivers votes. 

 
PLEASE GIVE CAREFUL THOUGHT WHO YOU WILL VOTE FOR ON TUESDAY...PLEASE THINK 
ABOUT WHERE THESE TWO MEN COME FROM AND WHERE THEIR HEARTS TRULY ARE.  THIS IS 
A CRUTIAL ELECTION AND WE AS AMERICANS MUST BE CONFIDENT THAT OUR BEST DAYS ARE 
AHEAD OF US WITH JOHN LEADING THE WAY!!!


  
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RE: [Histonet] Protect fingers during Microtomy

[Martin, Gary]

Do you mean she was barefoot, pregnant, and in the lab!! How could
you! 

-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Edwards,
R.E.
Sent: Tuesday, October 28, 2008 9:55 AM
To: 'Blazek, Linda'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Protect fingers during Microtomy

My wife, the best section cutter in the world worked right up to the
bitter end of her pregnancy with our third daughter, dropped an old
style steel knife and inadvertently gave herself a caesarean, beat that
histopeople!!!.



-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Blazek,
Linda
Sent: 28 October 2008 13:48
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Protect fingers during Microtomy

Besides a few little nicks that we all get, about 10 years ago after
having worked in histology for almost 25 years I was finally in a lab
with windows!  Early one morning as I was sitting there cutting with my
back to the window, dawn just breaking.  A head popped up outside the
window and the tech facing the window screamed just as I was running my
thumb over the block.  I jumped; the chuck came down and sliced into the
pad of my thumb down to the bone. I ran to the sink holding my thumb and
ran it under water.  It's pretty gross to see the bone in your thumb!
Funny thing, there was no pain until the ER doc filled it full of the
stuff to numb it.  Got lots of stitches and even now it sometimes hurts.

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email: [EMAIL PROTECTED]

 

-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Weems,
Joyce
Sent: Tuesday, October 28, 2008 9:28 AM
To: Telgenhoff, Dr. Dale; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Protect fingers during Microtomy

I've heard that tale, but didn't know who did it! 

I had a coworked who cut the pad off his thumb. We went to the ER, where
I learned he had put it in the trash. I went back and dug through the
paraffin scraps and got it - washed it in saline and Betadine.. The ER
dr glued it back on and he has a thumbprint again! 

When I caught myself on fire, cleaning with solvants too close to the
hot plate (the fumes ignited), he was the same guy who yelled, Get the
marshmallows - the supervisor's on fire!. I still get a good laugh
about that! J:)

And it's only Tues!! Trick or treat!!!   



Joyce Weems
Pathology Manager
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
Please note new phone and fax numbers
678-843-7376 - Phone
678-843-7831 - Fax

 

-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of
Telgenhoff, Dr. Dale
Sent: Tuesday, October 28, 2008 9:18 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Protect fingers during Microtomy

Not me, but the guy who trained me.  Remember the old knife sharpeners
that looked like record players?  He was removing the blade (they got
pretty oily) and it slipped.  His natural reaction was to grab for it,
slicing three fingers to the bone.  Made for a good cautionary tale...
 

Dale Telgenhoff, PhD
Assistant Professor
Tarleton State University
Clinical Laboratory Science
1501 Enderly Place
Fort Worth, TX 76104
[EMAIL PROTECTED]
817-926-1101 Ext. 7




From: [EMAIL PROTECTED] on behalf of Connolly,
Brett M
Sent: Tue 10/28/2008 8:10 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Protect fingers during Microtomy




 Me - 1982 - working in a S. Florida hospital I sliced open the base of
my thumb on the corner of a big steel blade as I was sectioning. Didn't
even notice it until I saw the blood dripping off my elbow.

I went to the ER and was sitting on a gurney with my hand submerged in a
bowl of betadine waiting to be stitched up. After what seemed like a
pretty long time,  I deftly fell off the gurney onto the floor tipping
the entire bowl all over onto myself.

Upon hearing the clamor of me hitting the floor and the metal bowl
banging around a rush of ER staff charged in and I got prompt service
thereafter. I still have the scar, but I never cut myself again :-)

Brett

Brett M. Connolly, Ph.D.
Research Fellow, Imaging Research
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
PH 215-652-2501 fax. 215-993-6803
e-mail. [EMAIL PROTECTED]


-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Jackie M
O'Connor
Sent: Monday, October 27, 2008 4:44 PM
To: Peter Carroll
Cc: histonet@lists.utsouthwestern.edu;
[EMAIL PROTECTED]
Subject: Re: [Histonet] Protect fingers during Microtomy

OK - time to poll - who has had sutures from microtomy?  Me!23 years

ago - 8 months pregnant, and not paying attention to what I was doing.
Oh
yeah, and one other time, I cut off the tip of my finger