[Histonet] IHC on Bones
Hello Histonet, Wondering what those of you that do IHC on bones do to ensure that the tissue remains on the slide during processing? Thanks, Sara McCabe HT(ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of the company. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PMS2 Mismatch Repair
Hello Histonetters, Wondering if anyone is using PMS2 Mismatch Repair Enzyme from Novocastra/Leica. Have tried varied dilutions and retrievals without any luck. Any suggestions would be great! Thanks! Sara McCabe, BS, HT (ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of the company. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CMV IHC
Happy Friday Histonetters! Anyone performing CMV by IHC? If so what are you using for a protocol? We ordered CMV from Cell Marque but they give no suggestions for a protocol. We are using the Leica Bond for our IHCs. Any input is greatly appreciated! Thanks! Sara McCabe, HT (ASCP) DuBois Regional Medical Center From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu [histonet-requ...@lists.utsouthwestern.edu] Sent: Friday, August 26, 2011 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 93, Issue 36 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Embedding Center Questions (Tim Wheelock) 2. Embedding competency and med techs running a Histo lab (D'Attilio, Shelley) 3. RE: AMPHYL (Sebree Linda A) 4. RE: Embedding Center Questions (Rathborne, Toni) 5. Jill's MSDS question: a tech's perspective (Cheryl) 6. RE: heavy duty microtome (Jack Ratliff) 7. RE: AMPHYL (McMahon, Loralee A) 8. AUTO: Gina Rodriguez is out of the office. (returning 09/06/2011) (gina.rodrig...@leica-microsystems.com) 9. OT: Hurricane Irene (Pam Barker) 10. RE: AMPHYL (Shirley A. Powell) 11. Re: Jill's MSDS question: a tech's perspective (Jill Cox) 12. Re: Jill's MSDS question: a tech's perspective (Emily Sours) 13. Re: Embedding process etc. (Bob Richmond) -- Message: 1 Date: Fri, 26 Aug 2011 09:54:27 -0400 From: Tim Wheelock twheel...@mclean.harvard.edu Subject: [Histonet] Embedding Center Questions To: histonet@lists.utsouthwestern.edu Message-ID: 4e57a593.90...@mclean.harvard.edu Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi All: My 23 year old Shandon Embedding Center may be ready to give up the ghost. It has had two repairs of the coolant system for the freezing plate over the last year. Now a thermo-resistor has burnt out, so that the wax reservoir with no long melt the wax. Does anyone know of a company or companies that lease/rent histology equipment? Are there companies who sell used equipment? Also, what do people recommend as far as a new embedding center, in case we can afford it? I am in the Boston area. In the meantime, maybe I can jury-rig something, since it is only the wax reservoir that is down. The cassette holding tank, hot plate, and freezing plate are OK for the moment. Maybe I can melt a few jars of wax, and use a transfer pipet to fill the molds? Any ideas? Thanks, Tim Tim Wheelock Assistant Director, Neuropathology Instructor In Neuroanatomy Harvard Brain Tissue Resource Center 203 Mailman Research Center McLean Hospital Belmont MA 02478 Phone: 617-855-3592 Fax: 617-855-3199 -- Message: 2 Date: Fri, 26 Aug 2011 08:57:35 -0500 From: D'Attilio, Shelley sdatt...@stormontvail.org Subject: [Histonet] Embedding competency and med techs running a Histo lab To: Histonet Listserv (E-mail) histonet@lists.utsouthwestern.edu Message-ID: b7244ac045486c4190de73f226f432d41bd69...@exchange-vs1.stormontvail.org Content-Type: text/plain; charset=iso-8859-1 Hi all, Thanks so much for the advice I received following my request for help on our embedding competency improvement project. I enjoyed the lively discussion that followed about non-histotechs managing a Histology lab and I appreciated all the kind comments and words of encouragement both on and off the listserv board. Regards, Shelley D'Attilio MT(ASCP) Manager, Chemistry, Cytology and Histology Dept. of Pathology and Laboratory Medicine Stormont-Vail HealthCare Topeka, Kansas NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your
[Histonet] RE: controls on patient slide
We always put our patient and control tissue on the same slide. We have many controls already cut and then we place the patient tissue below. If we are using blanks that were previously cut then we cut the control and place that below the patient tissue. We chose to not invest in the pre-marked slides. After processing we make a box with a red sharpie around the control tissue so there is no confusion as to which is the patient tissue and which is control. Sara McCabe, HT (ASCP) DuBois Regional Medical Center From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu [histonet-requ...@lists.utsouthwestern.edu] Sent: Thursday, July 21, 2011 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 92, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Assisting with Autopsies (Horn,Hazel) 2. Re: Leica Service Technician (Nicole Tatum) 3. RE: Leica Service Technician (Suresch, Donna L.) 4. Research microtomes (Adrienne Anderson) 5. Re: Research microtomes (Jack Ratliff) 6. Re: Research microtomes (Adrienne Anderson) 7. Re: Research microtomes (Jack Ratliff) 8. embalmers using formaldehyde - NYT story (Bob Richmond) 9. Re: Microtome draft shield (Joseph Saby) 10. Re: (Denise Piontek) 11. Re: Research microtomes (Adrienne Anderson) 12. Re: Research microtomes (Jack Ratliff) 13. Small Slide Scanner (Sheila Haas) 14. Fast Blue stabilization (carpj) -- Message: 1 Date: Wed, 20 Jul 2011 12:17:48 -0500 From: Horn,Hazel hor...@archildrens.org Subject: [Histonet] RE: Assisting with Autopsies To: 'Amy Self' as...@georgetownhospitalsystem.org, 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: 25a4de08332b19499904459f00aaacb7198b85a...@evs1.archildrens.org Content-Type: text/plain; charset=us-ascii We have an autopsy assistant and when he's not busy with autopsies (which is often) he works in our histology lab as an assistant!! Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, July 19, 2011 2:12 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Assisting with Autopsies Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility use histotechs or a diener to assist with the autopsy? Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: p16 on the Bond
Hi Nancy, We too use p16 on the Bond Max and Bond III using Leica ER 1 for 20 min. It is working great for us! Sara McCabe, HT (ASCP) DuBois Regional Medical Center From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu [histonet-requ...@lists.utsouthwestern.edu] Sent: Monday, July 18, 2011 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 92, Issue 23 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Invitation to connect on LinkedIn (Sheree Holmes via LinkedIn) 2. Water droplets on slides (Esther Peters) 3. RNAlater buffer fixation/embedding in OCT (Megan French) 4. positive staining on my secondary only controls...need a DAB superquench? (Megan French) 5. Lab humidity range (L White) 6. P16 on the BOND (Nancy Schmitt) 7. Re: Histobath revised (Johnson, Teri) 8. Alcian blue-safranin (Figliuolo, Leticia) 9. RE: Re: Histobath revised (Houston, Ronald) 10. RE: positive staining on my secondary onlycontrols...need a DAB superquench? (Elizabeth Chlipala) 11. RE: P16 on the BOND (Settembre, Dana) 12. Spirabrush (Gauch, Vicki) 13. Leica SELECTECH (Breeden, Sara) 14. acceptance criteria of IHC stains (Elizabeth Chlipala) 15. Peggy Wenk's job postings (Lee Peggy Wenk) -- Message: 1 Date: Sun, 17 Jul 2011 22:10:33 + (UTC) From: Sheree Holmes via LinkedIn mem...@linkedin.com Subject: [Histonet] Invitation to connect on LinkedIn To: David Edmondson histonet@lists.utsouthwestern.edu Message-ID: 1217399822.5174163.1310940633806.javamail@ela4-bed81.prod Content-Type: text/plain; charset=UTF-8 LinkedIn Sheree Holmes requested to add you as a connection on LinkedIn: -- David, I'd like to add you to my professional network on LinkedIn. - Sheree Accept invitation from Sheree Holmes http://www.linkedin.com/e/yvpgd1-gq8k7etn-f/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I133508382_13/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPclYOe3cUc3kPcP59bT9NtPlhgCBAbPgTc3cNdPcSdjcLrCBxbOYWrSlI/EML_comm_afe/ View invitation from Sheree Holmes http://www.linkedin.com/e/yvpgd1-gq8k7etn-f/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I133508382_13/3cNnP8UcPwMdjcPckALqnpPbOYWrSlI/svi/ -- DID YOU KNOW your LinkedIn profile helps you control your public image when people search for you? Setting your profile as public means your LinkedIn profile will come up when people enter your name in leading search engines. Take control of your image! http://www.linkedin.com/e/yvpgd1-gq8k7etn-f/ewp/inv-22/ -- (c) 2011, LinkedIn Corporation -- Message: 2 Date: Sun, 17 Jul 2011 19:10:36 -0400 From: Esther Peters epete...@gmu.edu Subject: [Histonet] Water droplets on slides To: Histonet@lists.utsouthwestern.edu Message-ID: 4e236bec.90...@gmu.edu Content-Type: text/plain; charset=ISO-8859-1; format=flowed I am having problems with water droplets appearing on slides when transferring the rack from the last 100% dehydrant (Richard-Allan) to either SafeClear II or xylenes. I saw that some of you had noted this problem might be in the xylene substitute, but switching to xylene seemed to solve it. I am wondering if the 100% isn't? I have poured immediately before use from freshly opened bottles, and still have the problem. Would appreciate any help (of course, the humidity is high right now, but...)! -- Esther C. Peters, Ph.D., Assistant Professor; Department of Environmental Science Policy; George Mason University -- Message: 3 Date: Mon, 18 Jul 2011 13:43:25 +1000 From: Megan French megan.fre...@mcri.edu.au Subject: [Histonet] RNAlater buffer fixation/embedding in OCT To: histonet@lists.utsouthwestern.edu Message-ID: de098b84182f9b4f966ee97d926888d9b48...@murmx.mcri.edu.au Content-Type: text/plain; charset=us-ascii Has anyone successful fixed embedded tissues in OCT after using RNAlater buffer? I've found a paper that says this doesn't affect tissue integrity but I need for info regarding protocol (To defrost? Fixative? Timing? Temperature Etc) ...and more than anything feedback from someone who has used/uses this product. Product:
