Re: [Histonet] [EXTERNAL Sender] histologists assisting with transplants
when I worked, I did a lot of histology on both native and donor livers. I would not have used the term assisting with a liver transplant but on rare occasions, I had requests for a rapid Oil red O for fat on the donor liver In order for the pathologist to better determine the percentage of fat in the donor liver. Too much fat and it might not be considered for a transplant. Maybe it is just a matter of terminology. After all the pathologist and surgeon called her in at 3am and may have thanked her for her assistance. In 30 years I may have done 3 rapid ORO's on donor livers Rena Fail retired On Thu, Feb 13, 2020 at 11:44 AM Hannen, Valerie via Histonet < histonet@lists.utsouthwestern.edu> wrote: > > > -Original Message- > From: Hannen, Valerie > Sent: Thursday, February 13, 2020 10:50 AM > To: 'Eileen Akemi Allison' > Subject: RE: [EXTERNAL Sender] [Histonet] histologists assisting with > transplants > > The only way that I have been involved with anything to do with any kind > of transplant was to do a FS on the donor tissue, to ensure that it was a > good transplantable organ. > > > > Valerie Hannen,MLT(ASCP),HTL,SU (FL) > Section Chief, Histology > Parrish Medical Center > 951 N. Washington Ave. > Titusville,Florida 32796 > T: (321)268-6333 ext. 7506 > F: (321) 268-6149 > valerie.han...@parrishmed.com > www.parrishmed.com > > > > -Original Message- > From: Eileen Akemi Allison via Histonet [mailto: > histonet@lists.utsouthwestern.edu] > Sent: Thursday, February 13, 2020 7:42 AM > To: Histonet > Subject: [EXTERNAL Sender] [Histonet] histologists assisting with > transplants > > > This message came from an external source. Please do not click links or > open attachments if unexpected or unusual. > > Begin Original Message: > > -- > Good morning histoland! > Just curious, this is definitely a 1st for me. Received a call this > morning from my histotech who is on call this week that she was called in > at 3:30 AM to assist one our pathologists with a liver transplant. In all > my years working in this field, histologists have never had anything to do > with transplants. Have any of you out there had anything to do with > transplants? > > Akemi Allison, BS, HTL > Histology Supervisor > UMC El Paso, TX > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PaPH0eDnAlaiSnq7Yv-U!ojZKih_v3GjCqE-m83bT6ADWtlPNJ4iLqZrKlTfh3RR3BcI2M-vI5hyWle4K4LiEDVspJQ$ > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] On the lighter side...
37 years On Friday, August 8, 2014, Jim Burchette jburc...@gmail.com wrote: 40 blessed years that I would not trade for anything. I have met so many wonderful people (well, maybe a few not so wonderful...) and have learned so much. The good Lord has blessed me with a talent that I feel has allowed me to make a contribution to society. On Friday, August 8, 2014, White, Lisa M. lisa.whi...@va.gov javascript:; wrote: 15 years as HT...26 years total in medicine started as an EMT and after some time discovered that patients in a jar is a blessing J thus the transfer to Pathology..and that is the rest of the story. Lisa White, HT(ASCP) Classification: Internal and External Use\\Not VA Sensitive This message has been categorized by White, Lisa M. on Friday, August 08, 2014 at 7:32:08 AM in accordance with VA Handbook 6500 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu javascript:; javascript:; http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jim Burchette Fly Fishing Bum *'(((* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu javascript:; http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Peggy Wenk's passing
So sorry to hear of Peggy passing may she have peace. At last On Monday, July 28, 2014, Lee Peggy Wenk lpw...@sbcglobal.net wrote: It is my sad duty to tell you that Peggy Wenk passed away peacefully Saturday morning. Her husband and companion of 37 years and her sister (from Bend,OR) were there. As you know Peggy had a large influence in the world of Histology. She also put herself into her church serving over the years as a nursery school director, Lay Eucharistic Minister and on the vestry. She sang enthusiastically in the choir and played in the newly formed bell choir. Because of our love of books, we both volunteered at the local library. We helped collect books, sort them and then helped to sell them; raising funds for the library's use. There will be two memorial services: one here in Michigan for all her local family and coworkers, the second in southern California (a burial at sea). We are asking that no flowers be sent; instead Peggy has specified (she and her sister planned the funeral several months ago) three different charitable organizations in lieu of flowers. St. Mary’s-in-the-Hills Episcopal Church (Peggy's church) Shades of Pink Foundation (financial aid to local breast cancer patients) Peggy A. Wenk Endowed Scholarship for Histotechnology at Oakland University Please respond to this email if you'd like more information on the services or on giving (or to l...@lpwenk.net javascript:;). Thanks Lee Wenk (Mr. Peggy Wenk;) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu javascript:; http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Liver bxs
Your description is consistent with the tissue having dried out. So observing their procedure may be helpful. Is it placed on gauze then placed in formalin ? That would dry it quickly. are they tending to the pt then placing the specimen in fprmalin, Rena Fail On Thu, Jun 19, 2014 at 9:03 AM, Mike Pence mpe...@grhs.net wrote: Ask to be present at the next liver bx time and see for yourself their handling of the specimen. Sounds like it set out for some time. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amanda Coscetti Sent: Wednesday, June 18, 2014 10:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Liver bxs Hi HistoWorld! Wondering if anyone has seen/heard of this as it has happened to us a couple of times in the past year and just happened again today! We had a liver bx come from Radiology and we processed it as usual but under the microscope our pathologist said it was unreadable, the edges had artifact, and it had inconsistent staining (on the HE, iron, and trichrome slides). The radiologist claims he put the specimen directly into formalin. All of our other bxs and tissue were fine. This was the case before when it happened and we have only had this problem with liver bxs! Any ideas why this may occur? Thanks y'all! Amanda Florence SC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] manual tissue processing protocol
Hi, One mm thick is tiny, 2 mm small 1 cm is rather thick. Since you are manually processing run one sample to test your protocol or 2 if you want to see the difference between the shorter method and the longer. *protocol. .Complete dehydration is dependent on the volume, type of tissue and more importantly, the thickness of the tissue. You can check the tissue before infiltrating with paraffin.to http://paraffin.to see if it has cleared, If it appears transparent (like a stained glass window.) proceed with paraffin infiltration. * *Rena Fail* On Wed, Feb 26, 2014 at 2:02 PM, Tyrone Genade tgen...@gmail.com wrote: Hello, I need some input on a manual tissue processing protocol I have received. (5 columns: reagents; tiny to large tissues...) Reagents\embryoes\small tissue\medium tissue\large tissue 70% EtOH\30 min--1hr\1 hr--ON\2hr--ON\4hr--ON 95% EtOH\30 min--1hr\1 hr\1hr\2hr 95% EtOH\30 min\30 min\1 hr\1 hr 100% EtOH\30 min\1 hr\30 min\1 hr 100% EtOH\10 min\30 min\30 min\1 hr toluene\30 min\1 hr\1hr\1 hr toluene\none\30 min\30 min\1 hr till clear parafin infiltration\1hr all parafin infiltration\1 hr\1 hr\2hr\2hr EM400\1 hr\1hr\1hr\2+ hr I would be replacing the toluene with methylbenzoate. Are there any anticipated issues? Is this a good protocol so far as producing good quality tissue for sectioning and analysis? I am not sure what is meant by small and large tissue. My fish are about 5 cm in length and about 1 cm thick. Would they be classed as small? Is it necessary using two different types of paraffin : Surgipath infiltrating paraffin Cat # 01400 (4-5 lb/bag) and Surgipath embedding paraffin? For the manual infiltration: would a beaker of paraffin on a 60oC hotplate and stirrer (on low) be OK? I would be fixing with PFA and decalcidying with 30% EDTA. Might refixing in the PFA after decal be a good idea to make sure the tissue integrity is maintained? I would probably stored fixed, decalsified samples in 70% EtOH until I can process fully. Any one think there would be complications from doing this? Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
The lab will run more efficiently if techs have forceps they are comfortable . I had 2 pair I cut and embedded with for over 20 years. They were longer with Thin pointy ends the tension was just right so my fingers didn't cramp and long enough so that I could see the tiny bxs without looking around my own hand. Though there are some people I worked with that I miss (I've retired) and some aspects of the job I miss, I miss my forceps most of all, Rena Fail On Wed, Aug 7, 2013 at 9:04 AM, Sanjeet Dhirubhai asanj...@yahoo.comwrote: Hi, I am trying lean up at the embedding system. We have issues where staff have their own preference in regards to working on a specific forceps. I am trying to standardize this process and eliminate the hassle of having different types of forceps. Can anyone help me. Thanks Regards, Sanjeet Dhirubhai - Supervisor Histology MLT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histonet mailing list submission: Jones' Methenamine Silver Stain
Anna, place your coplin jar with silver solution in a 65 degree waterbath5-10 minutes before your slides need to go in the sol. PreHeating the sol.in the waterbath and staining the slides in the waterbath will aid in reducing the time down to 10-15 minutes. make the methenamine- silver fresh. the silver and borax can be made ahead the methenamine should be made fresh each time.,Don;t check the slides until the tissue looks golden brown.your end point is when the elsatic tissue is black (wlastic stains1st with the silver), Bowman's capsule is black and the basement membrane of the glomerlus is golden brown to black. the temptation is to stop when the bm is defined. Make sure the bowmans capsule is black and the bm in the glom. is dark golden brown to black. BM in thhte skin stains rapidly much faster than thhat in thhe glom Rena fail On Mon, Jul 15, 2013 at 5:13 PM, Anna Huntley Coffey annamhunt...@gmail.com wrote: Dear Histonetters, I was wondering if anyone has experience with Jones' Methenamine Silver stain? We have attempted this stain several times, each time with a slight tweak to the protocols we found online. We found that it took much longer than expected (3-4 hours compared to the expected 1 hour) for the membranes to develop the expected black color every attempt we made. The few times we did see the black color develop in the membranes, the next step in the gold chloride solution (1 minute) washed away all the dark color in the kidney, leaving it a very light brown. However, we did notice that only the skin sample on our test slides retained the black color. Since we ultimately need this stain for kidney samples, this didn't do us any good. We tried thinner sections (2 micron), acid washed glassware, brand new reagents, and multiple protocols with no luck. I would greatly appreciate any advice as to what we could try differently or if anyone else has experienced these same problems. Thank you! Anna ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!
Oil Red O is a stain for fat, Alcohol dissolves fat Rehydrating won't help. You can't replace the fat. Rena Fail On Tue, Nov 13, 2012 at 11:52 AM, z o n k e d zon...@gmail.com wrote: Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: special stains for demonstrating intra cytoplasmic DNA viral inclusion bodies
Try Lendrum'sstain for inclusion bodies. Rena Fail On Sat, Oct 13, 2012 at 2:48 PM, anjan kumar drvet_an...@hotmail.comwrote: Would like to know special stains for demonstrating intra cytoplasmic viral inclusion bodies tried with shorrs stain giving lot of red color Anybody can suggest alternative method excluding immunohistochemistry! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
Can't beat phone books. Cheap, torn in half they are wider than a kimwipe, you're recycling, and providing therapy in the form of stress relief obtained tearing up phone books. Rena On Tue, Jul 31, 2012 at 2:23 PM, Paula Pierce cont...@excaliburpathology.com wrote: Greetings, does anyone know of a less expensive substitute for Kim-wipes? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Counter stain for PAS for fungus (Steve McClain)
earlier I recommended using light green yellowish as a counterstain for Pas for fungus. Some Pathlogists prefer the green counterstain when staining with PAS for fungus. Using a 5% chromic acid solution as the oxidizer and controlling the time and temperature will eliminate reactive aldehydes in all but the structures that have the greatest concentration of carbohydrate Using chromic acid makes it much easier to detect fungus Histologic Dec.2005 XXXVIII(2):35 Rena Fail the first pathologist I worked for had AB-Pas stains done every day and you are right they are wickedly good. On Thu, Jul 26, 2012 at 4:31 PM, Steve McClain ste...@mcclainlab.comwrote: Change all your reagents. Change periodic acid every week at least. PAS unlike many other stains is a chemical reaction and is sensitive to oxidizer Personally I see little advantage in fast green, and a common downside is overstaining in many labs, hiding fungus and basement membrane. Faint Hematoxylin is acceptable counterstain. For certain applications, e.g., dual staining for carbohydrate and mucin, by adding AlcianBlue for 40 seconds is wickedly good. Dis/Advantage being dual staining methods impose two charges. Too bad. Instead of separate stains for mucin and carbohydrate, do them both every day and get good at it. We rarely do a straight PAS any more, either PAS+AB+H or PAS+AB Subtract the second charge where needed. The two analytes (carbohydrate and mucin) are different, yet the interpretation is synergistic. Where you see mucin glommed around a hypha-like structure it is. AB also demonstrates conidia and microconidia and internal structure in many fungi. AlcianBlue also stains bacteria, making it useful where polymicrobial (fungal and bacterial) infections are common. Consider it your biofilm assay. Many fungi produce mucin making PAS-AB with hema a superb method for detection. PAS-AB sine hema is considerably more sensitive, especially with pigmented fungal species, but interpretation of PAS-AB without Hema is more difficult for pathologists. Steedman wrote the original description , in 1951 and it remains the best paper. Steve Steve A. McClain, MD McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tennessee BB
Okay 30 years is too long in histology! I should have retired sooner than I did,. I saw this subject heading as a new stain for bacteria instead of an ad for a bed and breakfast in Tennessee. Rena Fail ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] counter stain for PAS for fungus
I have used a working solution light green Sf yellowish C,I. # 42095 and the only time I had problems was when someone left out the glacial acetic acid in the stock, Stock is light green SF yellowish 0.2 gm,distilled water 100.0 ml, 0.2 ml glacial acetic acid. Working is 10.0 ml of stock, 50.0 ml distilled water Rena Fail On Mon, Jul 23, 2012 at 12:11 PM, Michele Carr michelecar...@yahoo.comwrote: Hi everyone I am having a problem with the fast green counterstain for the PAS for fungus. The fast green stain is simply not staining anymore no matter how long I leave it on the specimen. Do you have any suggestions on how to correct this or perhaps recommendations for a different counterstain. Our protocol is as follows: Periodic Acid 7 min quick rinse Schiff solution 20min wash for 10min Fast green stain 1 min Any help is appreciated. Thanks in advance, Michele Carr Medical Laboratory Services ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Jim Burchette
No muss, no fuss just a generous heart always ready and willing to share. You made life easier in the lab because of your generosity. Thank you. Congratulations on your retirement! Rena Fail HRT (Happily retired Histotech) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Elastic Stain
Have you tried Resorcin fucshin HE? 1 g RF 100 ml 70% ethanol 1 ml HCL Stain in RF solution 15-30 minutes , according to depth desired wash off excess stain in 95% alcohol Wash in tap water 3-5 minutes Follow with routine HE Rena Fail On Thu, May 24, 2012 at 11:37 AM, Vickroy, Jim vickroy@mhsil.comwrote: We are still looking for an Elastic/HE stain procedure for lung tissue. Please submit procedures to Jim Vickroy. vickroy@mhsil.commailto: vickroy@mhsil.com A few of you have responded with some suggestions but we still have not found the right procedure. Currently we stain for elastic with Verhoeff's Elastic Stain and use Van Gieson's stain as a counterstain and the pathologist would like us to use a hematoxylin and eosin counterstain instead. We have tried to use the HE as a counterstain but it is still not where he wants it to be. Does anybody have some experience with this that they would like to share? Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES
It's pretty much up to you,. your boss, the pathologists what colors you use. Red for rushes for example. The colors help identify a group of specimens quickly, then they can be sorted by number. This is especially useful if your SOP is to cut groups of specimens in a specific order. Rena Fail On Fri, Mar 23, 2012 at 8:42 AM, Bernadette del Rosario badzros...@yahoo.com wrote: Good day histonetters.We are a new university hospital and setting up histopatholology lab.I used to work with white biopsy cassettes only but not technicolors.Got this boss who ask me protocols on colored cassettes etc...No idea about this.Is there any standard pattern which i can just base and copy (example skin-yellow;breast-pink etc..)Im trying to surf in the net but cant find..Please someone help me??? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Xylene and Preggers
Human resources ultimately represents the company . A pregnant employee should be wearing ppe when handling xylene. MSDS forms are accessible to all employees, just a click away on the computer. I can understand wanting to keep some things private until you are ready to communicate them, but when you work with chemicals such as xylene and you are pregnant making sure you are wearing the proper PPE and the air quality meets OSHA standards comes first. I don't think the information communicated by HR to your supervisors should become common knowledge, but there is an obligation to protect the employee as well as the employer, Rena Fail On Mon, Jan 23, 2012 at 12:26 PM, Sarah Dysart sdys...@mirnarx.com wrote: Hey all, so 2 things... A. Does anyone have anything saying that .75% (yes less than 1) is an acceptable exposure limit for a pregnant person and B. Does HR have the right to tell people that you are pregnant after you ask them questions (ie. Your manager, and all the way up??) Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Amazing
0.5% HCL acid ih 70 % ethanol Rena fail On Thu, Jan 5, 2012 at 11:25 AM, Burton, Lynn lynn.bur...@illinois.govwrote: I still use that. It is 70% with 5mL of hydrochloric acid if making 1L. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 From: histonet-boun...@lists.utsouthwestern.edu [ histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart [ sdys...@mirnarx.com] Sent: Thursday, January 05, 2012 9:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amazing I find it amazing sometimes when you don't do something for awhile how quickly your brain throws the information away. That being said...I know back in the day when I was learning histology we used to make our own acid alcohol solution (now where I am had a butt load of Clearifier so I was using that up). I don't want to buy that stuff anymore as making the solution is way cheaper and works just as well. I want to say it was like a 1% acid solution in alcohol?? What was the acid? For some reason my brain says glacial acetic...but time has made me forget. Is the alcohol you mix it in 100% or something lower with a water content to it? Please help my alzheimers =) Happy Thursday!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cutting speed
Teresa, How do your sections look? Good quality well stained sections are vital. Speed will come with experience. When it becomes second nature to know when a block is cold enough to get good sections without cracks from being too cold or when a bloody piece of tissue is moistened enough toget sections without swollen cells your speed will increase. You will have been working a month by the time the tech goes on vacation and will have the workflow down by then. Don't worry so much. no one will expect you to be as fast as a tech with years of experience. Good luck rena Fail On Sat, Dec 31, 2011 at 8:44 AM, Teresa Moore tmoor...@gmail.com wrote: I graduated from a histology program in June/11 and just got a job a week ago. My speed on the microtome is not great. Everyone says it takes time but I feel my technique may be wrong. To make matters worse the only other histotech in the lab is going on vacation the third week of January and I will be alone! I don't have the overall flow of the lab down yet and have no idea how they expect me to handle the cutting all by myself. My biggest concern is my cutting speed right now. How long does it take (approx) to do 40 blocks an hour. Currently, I'm about half that! I'm panicking and I've only been on the job 8 days. Help!!! -- Teresa Moore ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] vanGieson-Trichrome staining protocol
Are you perhaps refering to the Verhoff's Van Gieson, if you are trying to stain elastic then a trichrome it can be done. The silver should be cloudy like a slightly dirty window , not milky generally if you add enough ammonia to the silver and sodium hydroxide mixture to render it completely clear then you will probably have added too much ammonia. Rena Fail - Original Message From: Doughty, Adele adoug...@lrri.org To: histonet@lists.utsouthwestern.edu Sent: Mon, July 19, 2010 10:29:19 AM Subject: [Histonet] vanGieson-Trichrome staining protocol Is there anyone out there that has done these two stains together? If so, I need assistance with a protocol. Also I am doing a reticulum stain and I am having a time with the ammoniacal silver solution, I can get the solution clear but when it comes to the cloudy do they mean milky cloudy? Thank you Adele ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
Are you washing the bone marrows before processing? Years ago ;) when I used acidic zenkers we washed the BM before processing then post fixed in formalin on the processor. The overnight processing was formalin until the machine started 1 hour in 70% ethanol, an hour in 3 changes 95% ethanol, 3, changes 100% ethanol, 3 changes paraffin. for rush processing it was 20 minutes in each. THe bone marrows were cut at 3 microns. i didn't have a problem with tissue washing off. Rena Fail - Original Message From: Johnson, Nacaela nacaela.john...@usoncology.com To: histonet@lists.utsouthwestern.edu Sent: Wed, June 16, 2010 4:53:22 PM Subject: [Histonet] (no subject) Does anyone know a good overnight processing protocol for bone marrows fixed in Acid Zinc Formalin? My tissue is washing off the slides. I have tried adhesive slides and they do not help. I am currently using a processing protocol that many use for bone marrows. I think the AZF may be dehydrating the tissue and this process protocol is making it worse. Any suggestions for a protocol? I am not able to change fixatives. Thanks, Nacaela Johnson Histology Technician KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: nacaela.john...@usoncology.com /preThe contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.brOnly the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone./pre ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] possible AFB contaminant
Are you using tubing on your dispensers for your DI water? They can be a source of contaminant. You can also have cross contaminantion from your control slide during dehydration and hydration as well as during staining.Do not use the same set up for contrpl and pt slide. Lay slides flat for hand staining Rena Fail - Original Message From: debra.or...@uchospitals.edu debra.or...@uchospitals.edu To: histonet@lists.utsouthwestern.edu Sent: Thu, May 20, 2010 3:41:09 PM Subject: [Histonet] possible AFB contaminant Please help, we have had an increase in demands for repeats on our AFB stain. Our pathologists have been noticing what they believe are false positives on our slides. We perform our AFBs on the Ventana Nexus and some are done by hand. Because of this problem, we have been doing side by side comparisons using the Nexus and hand stain. We still have complaints. We have made new reagents, changed kits, tested our DI water sytem and have different techs cutting using different waterbaths. Any advice or comments would be greatly appreciated. Thanks Debi This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Freezing spray artifact
Erin, Holding the can too close and/or spraying for a prolonged period both cause freeze artifact. Look at your block, it will not have a smooth look to the paraffin. it will have lines it much like a cracked piece of glass. the tissue will appear cracked on the waterbath and will even separate along these lines. The cracks can be seen microscopically amd interfere with the architecture of the tissue. Rena Fail - Original Message From: Martin, Erin erin.mar...@ucsf.edu To: histonet histonet@lists.utsouthwestern.edu Sent: Wed, May 19, 2010 12:09:22 PM Subject: [Histonet] Freezing spray artifact Has anyone run into a problem or artifact from freezing spray? I think we may be having a problem with it but I can't find any pictures or descriptions of what it looks like. Thanks in advance, Erin Erin Martin, Histology Supervisor UCSF Department of Dermatopathology 415-353-7248 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] alcian blue van gieson stain
Alcian Blue first - Original Message From: Perry, Margaret margaret.pe...@sdstate.edu To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Fri, March 12, 2010 9:30:40 AM Subject: [Histonet] alcian blue van gieson stain We want to try the double stain alcian blue Van gieson. Do you stain for the alcian blue first and then the Van gieson? We do both stains but I am unsure of how to put them together. Margaret Perry South Dakota State University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fw: Histo story
- Forwarded Message From: Rena Fail renaf...@bellsouth.net To: nmhi...@comcast.net Sent: Thu, March 11, 2010 1:48:12 PM Subject: Histo story A single parent at 29 with 4 children, unpredictable child support, and only a high school education, I could only get minimum wage jobs. I started looking into federally funded educational programs. the obvious, becoming a nurse, but that program had met its quota. I wanted to work in the health field so the adviser started going through the college catalog to see what other 1-2 year programs were offered in the health field. When we read the very short but descriptive paragraph for histology I knew that was it, that was what I wanted to do, I couldn't wait. So it was back to school at 31 and for 30 years I practiced the Art of Histology. From that short paragraph to a career in a profession I loved and for which I never loss enthusiasm or wonder. For nearly 20 years I worked for the department head Gordon R. Hennigar who taught me to trust my instincts and always always add to my knowledge, read the old books and publications as well as the new. To do the very best work every day so that the pathologist could do his best for the patient. I was also privileged to have worked in the same department with Drs. Joseph McManus and Sam Spicer. Both of these researchers would not hesitate to answer a techs questions. Though Sam Spicer usually responded with copies of articles he had written and a mini lecture. The last years before my retirement my immediate boss was Vinnie who taught me to put pen to paper to share with others. i learned to write manuals, develop stain procedures, play with dyes, put out fires, help pathologists, work with residents and help patients, all because of one little paragraph in a college catalog. Rena Fail Retired from Medical University o fsouth Carolina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] restaining IHC slides
you can use the same slides , repeating all the steps except the antigen retrieval ( if it is the same ) Ideally you should use new slides, but on tiny bxs, outside slides, or very limited area of interest, that is not aways possible. Rena Fail - Original Message From: Thomas Pier t...@medicine.wisc.edu To: histonet@lists.utsouthwestern.edu Sent: Wed, December 16, 2009 1:44:25 PM Subject: [Histonet] restaining IHC slides Hello Histonetters, I ran into an issue when staining some TMA slides the other day. This question isn't about how to fix the staining, or lack there of. I would like to know whether or not it is possible to repeat the same protocol on the same slides after removing the coverslips. If any of you have any experience with this sort of thing, please let me know. Tom Pier ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] rhodanin stain fading
Alcohol will cause the stain to fade, even the small amt. that is carried over to the xylene over the course of the day. Make sure your xylene is fresh and coverslip immediately after staining Rena Fail - Original Message From: Gudrun Lang gu.l...@gmx.at To: histonet@lists.utsouthwestern.edu Sent: Fri, November 20, 2009 2:16:49 PM Subject: [Histonet] rhodanin stain fading Hi! Yesterday I did a rhodine stain for copper. Immediatly after staining I saw not many but distinct red granula in hepatocytes. I am new to this stain, so I was happy (and a little bit proud), that it had worked. I also stained one slide over night for comparison. Today morning the first stained slides has faded and not the smallest bit of red colour could be seen. Is this a common problem? What causes the fast fading? Gudrun ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
