Re: [Histonet] Detection Systems

[Sally Price via Histonet]

Like Amos, I have extensive experience in working with biotin/streptavidin
(BSA) detection systems, and use them primarily because, in my analyses,
such reagents cost about half as much as polymers kits in the same volume
containers.  In addition, knowing that endogenous biotin can be problematic
in a small number of different tissues, our (clinical) lab simply avoids
using BSA in those situations.  As required by CAP standards, our IHC
policy acknowledges that we’ve conducted the necessary testing to determine
if/when endogenous biotin makes interpretation challenging, and refrain
from doing so when applicable.

Finally, although I don’t agree with Carl’s belief that one MUST use the
DAB chromogen+substrate components offered by the same vendor as the
detection components, we DO purchase such a reagents from the same
manufacturer simply because they’re more inclined to assist us in
troubleshooting if we run into a problem with EITHER reagent.
Sally


On Sun, Feb 6, 2022 at 2:40 PM Hobbs, Carl via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> As I stated:
> Polymer systems only work GREAT when you also use the DAB in the kit
> You pay dearly for it
> Check out my comparison in Histonet Images
> Carl
> Carl Hobbs FIBMS
> Histology and Imaging Manager
> Wolfson CARD
> Guys Campus, London Bridge
> Kings College London
> London
> SE1 1UL
>
>
>
> 020 7848 6810
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
-- 
Sally Price
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Antibody diluent

[Sally Price via Histonet]

Maria:
Personally, I believe in using the right tool for the job, and have learned
a lot about the science of IHC from Biocare.  I recently received an email
from them with a link to download a paper from their website that’s all
about diluents and I found this information very useful.  I would encourage
others to review this information if they want to learn why using a
universal reagent best option.
Sally--
Sally Price
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] ADH5 multiplex procedure

[Sally Price via Histonet]

-- Forwarded message --
From: "Kurth Virginia L." 
To: "histonet@lists.utsouthwestern.edu" 

Virginia:
I’m pretty certain that the ADH5 multiplex procedure is performed at CSI
Labs in the Atlanta area.  And I don’t think that biocare refers to it by
that name any more either - I think they just spell out all the antigens
that the antibodies in this cocktail can identify, like CK-5/14+p63+CK-7/18.

Sally

- - - - - - - - - - -

Date: Tue, 15 Oct 2019 14:48:40

Subject: [Histonet] ADH5
Does anyone know if there is a reference lab that performs ADH5.
(particular the BioCare multiplex stain?)

Thanks Ginny--
Sally Price
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Histonet Digest, Vol 167, Issue 12

[Sally Price via Histonet]

All:
I beg to differ with what seems to be the general consensus, but the Dako
handbook is not really representative of the available technology within
the IHC field and shouldn't be thought of as a prescription for
success.  All the Dako book addresses is Dako's methods, reagents and
instruments, which is pretty biased.  In order to properly prepare for the
ASCPs exam, one needs to think beyond reagents and automated systems and
REALLY understand the underlying science.  I've attended a number of
seminars on this subject and learned that, especially because of recent
changes to this test, one also needs to understand the clinical application
of these procedures.  More and more we're called upon to provide
pathologists with support in the way of procedure validation and
troubleshooting and we need to learn a great deal more than can be learned
from reading one vendor's publication.
+Sally
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] South Carolina Society for Histotechnology Fall Expo

[Sally Price via Histonet]

Hey folks –  I'm definitely not as good at promoting a meeting as Billie
Zimmerman is but I wouldn't want anyone to overlook their opportunity to
participate in the SCSH's Expo, which will be held the weekend of November
4 - 6 in beautiful Pawleys Island, just a short drive north of Charleston
and south of Myrtle Beach.  This may be your last chance to obtain
much-needed CEU's before the end of the year,  unless you enjoy those
boring on-line programs.  For more information, please contact SCSH's
President, Chad McMahan, who can be reached at < clmc...@clemson.edu >.
Hope to see y'all there!

-- 
Sally Price
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] South Carolina Society for Histotechnology's Fall Expo

[Sally Price via Histonet]

Greetings HistoNetters - In the event that you couldn't make one of
the state-society meetings that were held in the Spring or the NSH
Symposium/Convention just a few weeks ago, I hope you'll consider attending
the South Carolina Society for Histotechnology's Fall Expo, which will be
held Friday, November 4th through Sunday, November 6th at the Litchfield
Beach and Golf Resort in Pawley's Island - which is right between Myrtle
Beach and Charleston.

Our meeting will feature nationally recognized speakers including Liz
Chlipala, B.S., HTL(ASCP), former South Carolinian Joe Myers, M.S.,
CT(ASCP)QIHC, and Bill DeSalvo, HT(ASCP) - who are not only knowledgeable
in their respective fields, but also quite entertaining...or so I'm told.

For more information on this event, please contact the SCSH's President,
Chad McMahan at: < clmc...@clemson.edu >

Hope to see ya'll there!
-- 
Sally Price
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Tissue Controls (Deceptive)

[Sally Price via Histonet]

Not cool Rachel.  I took the survey assuming that it was intended and might 
help a colleage, but it's nothing but a marketing ploy, which are strongly 
discouraged on this forum.

 Original message 
From histonet-requ...@lists.utsouthwestern.edu 
Date: 06/15/2016  1:00 PM  (GMT-05:00) 
To histonet@lists.utsouthwestern.edu 
Subject Histonet Digest, Vol 151, Issue 14 
 
-

Message: 1
Date: Tue, 14 Jun 2016 14:09:16 -0400
From: Rachel M Gonzalez 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] Tissue controls
Message-ID: