Re: [Histonet] John Caggiano’s passing

[Shirley A. Powell via Histonet]

Oh my goodness, my heart is so heavy.  I love John and Joe and all the Caggiano 
family.  They were so helpful with all the state societies and supporteted us 
consistently through our early years and on into the later ones as well.  They 
are such a part of our profession and I will miss John so much.  So sorry Joe 
and Caggiano family.
Thanks for letting us know.

Shirley Powell
Now "Retired" HTL

From: Akemi Allison via Histonet 
Sent: Friday, November 10, 2023 4:36 PM
To: Histonet 
Subject: [Histonet] John Caggiano’s passing

Dear HistoPeeps:
I have some sad news to share with the histology/pathology community. Joe 
Caggiano informed me Monday that his brother John passed away this last Sunday 
night.  John Caggiano, founder, President and CEO of Poly Scientific R & D 
Corp, died quietly in his sleep after a long illness. John and Poly Scientific 
were early and longtime supporters of NSH, (National Society for 
Histotechnologists). He was always supportive, generous, and it was a pleasure 
to spend time with him. John was a great friend and he will be greatly missed. 
RIP John.

Regards,
Akemi Allison-Tacha, BS, HT/HTL (ASCP)



Sent from my iPhone
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Re: [Histonet] IHC staining of tendons and cartilage

[Shirley A. Powell via Histonet]

Hi Charles, 

Shirley Powell here in humid Georgia.  I ran an IHC reference lab here for many 
years.  I had a problem with using charged slides for a lot of the tissues I 
processed.  I used manual and automation methods.  My tissues were washing off 
a lot.  I changed to an adhesive for the water bath called Sta-On and I think 
Surgipath was the company that made it.  Surgipath was bought out by Leica but 
they still sold it.  Sta-On was the best adhesive I had found and that worked 
for me for many years.  Whenever I do IHC that is what I use, especially bone, 
cartilage, bloody specimens, autopsy tissues, they stay on better.  Some other 
companies may be selling it now, like VWR/Avantor.  Just Google it. 
 
Shirley

Shirley Powell, HTL(ASCP)
Technical Director Histology Curricular Support Laboratory
Pathology Department
Mercer University School of Medicine
powell...@mercer.edu
Phone: 478-301-2374
https://medicine.mercer.edu/

-Original Message-
From: Charles Riley via Histonet  
Sent: Tuesday, March 21, 2023 2:31 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC staining of tendons and cartilage

Hello all,

I am in a new position and it will potentially require doing a lot of IHC 
testing on cartilage and tendon samples. I have done some practice runs on my 
automated stainer and manually and am running into issues with the tissue 
sections falling off completely or folding over on itself during each process.

If anyone does staining like this routinely and has some pointers/tricks to try 
to get the samples to adhere to the slides better it would be greatly 
appreciated.

I have tried using charged slides from a variety of vendors and get similar 
results across the board.
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[Histonet] Georgia Society for Histotechnology 50th Anniversary

[Shirley A. Powell via Histonet]

Hi Histotechs,
Please join us at Sea Palms Resort on St Simons Island, GA for our 50th 
anniversary meeting.  The links to the program and registration are below.
Rooms will go fast so make your reservations soon.  The hotel rates and phone 
number to call are on our website at 
www.georgiahistotech.org<http://www.georgiahistotech.org>
These are the Program Schedule link and the Registration Form link.

50th Anniversary Program 
Schedule<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fgeorgiahistotech.org%2F2023-summit-schedule%2F=05%7C01%7Cpowell_sa%40mercer.edu%7Cc028cfd452d84bf5482c08db1ea9ee65%7C4fb34d2889b247109bcc30824d17fc30%7C0%7C0%7C638137487437407460%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C=8QpA0x0oHtguGtBDXVaYw9LtgFx1bhPPJknEqhELEW8%3D=0>

50th Anniversary Registration 
Form<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fform.jotform.com%2F223597690788174=05%7C01%7Cpowell_sa%40mercer.edu%7Cc028cfd452d84bf5482c08db1ea9ee65%7C4fb34d2889b247109bcc30824d17fc30%7C0%7C0%7C638137487437407460%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C=IP50aYAx5H2bgoVJyJ5vKOkWQy5x2z2G1j5jYRyce8M%3D=0>

Look forward to seeing ya'll there.
Shirley
Shirley Powell, HTL(ASCP)
Technical Director Histology Curricular Support Laboratory
Pathology Department
Mercer University School of Medicine
powell...@mercer.edu<mailto:powell...@mercer.edu>


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Re: [Histonet] Molds- cold vs warm

[Shirley A. Powell via Histonet]

Well said Jay.
Thanks, 
Shirley Powell


-Original Message-
From: Jay Lundgren via Histonet  
Sent: Friday, September 9, 2022 5:37 PM
To: Naira Margaryan 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Molds- cold vs warm

Whoever is telling you to use cold molds needs to go back to clown college.

That is totally, 100%, absolutely, wrong.

There is some debate as to embed "wet" (cassettes submerged in paraffin
bath) or "dry", and I will accept either, as mostly a matter of personal 
preference.  BUT, in both of these cases, the molds are hot.

I have been a Histotech for five decades, trained at Armed Forces Institute of 
Pathology (back when that used to mean something) and I have NEVER seen anyone 
using cold molds.

It is a guaranteed way to get cold fractures and cracks in your blocks, or to 
pop tissue out when you are cutting, which might be irretrievable. Just think 
how much time all those re-embedded blocks are going to save you!

Also, you won't be able to easily re-position specimens in the block, to put 
them "on edge" or "on end", for example.  The tissue will instantly stick to 
the cold mold.  And if you want to re-position it, guess what, you'll have to 
warm the mold up to get the tissue unstuck.  How's that (non-existent anyway) 
time savings now?


If you want to prove to whatever jackass suggested this that they are wrong, 
get a big stack of every histopathology textbook you can find.
There is nothing in any of them talking about paraffin embedding with cold 
molds.

As a matter of fact, every single textbook will specify molds at the same temp 
as paraffin.

Anyway, it doesn't even make sense, thermodynamically.  Heat travels from hot 
to cold.  Those "cold" molds will be the same temperature as the paraffin, 
almost instantly. Did it take a tiny amount of heat out of the hot paraffin? 
Yes, but not enough to noticeably cool the blocks faster. The amount of heat 
from the paraffin used to warm the mold is trivial compared to the total heat 
of the system. That's why cold plates have huge, noisy refrigeration units.  
You can't argue with thermodynamics.

If you are having trouble getting your blocks to release, use mold release!  
Viola!  
https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.statlab.com%2Fdata=05%7C01%7Cpowell_sa%40mercer.edu%7C84d81a9eddad4ba8159308da92ab72a7%7C4fb34d2889b247109bcc30824d17fc30%7C0%7C0%7C637983562331339740%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7Csdata=YDxIy7oMkibqOiMnl0D44LCG3XD0oKPY7jFHZBHBrsM%3Dreserved=0.
 I used to think it was superfluous, but now I consider it compulsory.  This is 
probably the answer to most of your issues.

I don't know who is suggesting using cold molds, but I can pretty much 
guarantee that it's a pathologist who thinks his slides are taking too long, 
and knows nothing about histopathology, or a lab manager, who knows nothing 
about histopathology.  This next part is directly to them.

To Whoever Suggested Cold Molds:  The answer to getting your slides out quicker 
is buying more equipment and hiring more techs, and holding everyone to 
standards (30 blocks/hr cutting, 60 blocks/hr embedding).
Making nonsensical, uninformed suggestions only exposes your ignorance.

Please feel free to show them this reply.

Sincerely,

Jay A. Lundgren, M.S., HTL (ASCP)
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Re: [Histonet] Intelsint tissue processors where are you?

[Shirley A. Powell via Histonet]

Hi Sannon, I have one.  Love it.  The cost is more reasonable that you can 
imagine.  
Shirley

-Original Message-
From: SEG via Histonet  
Sent: Wednesday, April 13, 2022 11:47 AM
To: histonet@lists.utsouthwestern.edu
Cc: Sean Draime 
Subject: [Histonet] Intelsint tissue processors where are you?

Hello out there Histology!

Are there any labs currently using the Intelsint processors in the US?  
Previously in the US under model names such as RTP300, RTPH300, RTP360, 
TRPH360, and RVG1.  Most recently Intelsint models are known by TP300, FTP300, 
ETP, and EFTP.

We recently met a few HTs who love these processors and want to hear more about 
what experiences have been had.  These processors are built like tanks, most 
not requiring service in 5-7 years despite no PMs!Let me know if you love 
yours?

If you're having trouble with yours please get in touch with me directly today 
to discuss how PSi can resolve them.  My email is 
shannon.go...@pathologyserviceinc.com 


Thanks,

Shannon Gower
Account Executive & Application Specialist Pathology Services Inc.

Toll Free:  866-398-9478
Cell:  727-515-8189
shannon.go...@pathologyserviceinc.com 





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Re: [Histonet] Dr Freida Carson

[Shirley A. Powell via Histonet]

Hi Donna, 
So sorry to hear of this. I am proud to have known her and been a recipient of 
her wealth of knowledge.  
She contributed so  much to the Histology Community.

Shirley 

Shirley Powell, HTL(ASCP)
Technical Director Histology Curricular Support Laboratory
Pathology Department
Mercer University School of Medicine
1550 College St, Macon, GA  31207
O: 478-301-2374/F:478-301-5489
medicine.mercer.edu




-Original Message-
From: Willis, Donna G via Histonet  
Sent: Wednesday, January 12, 2022 3:19 PM
To: Histonet (histonet@lists.utsouthwestern.edu) 

Subject: [Histonet] Dr Freida Carson

For those of you that are not on Facebook you may not already know that 
yesterday Freida Carson, PhD became one of our Histology Angels.  I had the 
privilege of having her as my Histology Educator long before she wrote her 
first edition of Histotechnology.  But more precious to me than having her as 
my educator was that she was a friend.  She will be missed.  Thank you Freida 
for all that you have given to the Histology Profession and to me.  Rest In 
Peace singing in the Angels Choir.

Donna Willis
Anatomic Pathology Manager
Baylor Scott Health
Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas 75246
214-820-2465 office|214-725-6184 mobile



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[Histonet] Tim's Retirement

[Shirley A. Powell via Histonet]

Congratulations Tim, 
Your world travels have been interesting and for us in the field very rewarding 
because we all have benefited from your education received in your professional 
career.  I envy your experiences in different parts of the world.  All of my 59 
years have been in the Macon, GA area.  Only 3 institutions, first 2 hospitals 
and the present one in a medical school.  I too hope you will keep hanging with 
us still working, you have a wealth of knowledge from which we all can benefit. 
 I wish you the best in your retirement. But don't forget about us and keep in 
touch. 

Have fun, 
Shirley

Shirley Powell, HTL(ASCP)
Technical Director Histology Curricular Support Laboratory
Pathology Department
Mercer University School of Medicine
1550 College St, Macon, GA  31207
O: 478-301-2374/F:478-301-5489
medicine.mercer.edu




-Original Message-
From: Morken, Timothy via Histonet  
Sent: Thursday, September 9, 2021 12:26 PM
To: Histonet 
Subject: [Histonet] Retirement in sight!


After 40 years in the lab I've decided to retire this year - in a week actually!

It has been an interesting 4 decades...

I started out in an EM lab after getting a degree in Physiology and then  
competing a 2 year EM course at Delta College in Stockton, CA - the only 
dedicated EM program at that time. I started out running a scanning EM lab for 
an electronics company looking at microchips but after a couple years moved to 
a hosptial lab in Fresno, CA running their EM lab. I was the only one, so from 
day one was the "Manager" of the lab! I did about 150 EM cases a year and in 
those days it was a mix of kidney and tumor cases - there was no IHC yet so 
some tumor diagnostics depended on EM. I did not have quite enough work to keep 
me busy so I started hanging out in the histology lab. As with many people in 
this field the day I started working there was the first I had heard of 
"histology."  At first it was helping set up grossing, coverslipping slides and 
doing immunofluorescence for the kidney cases (and taking "kodachromes" of the 
results! Does anyone under 30 know what a Kodachrome is?!). But then our 
director wanted to bring in IHC and so had a tech from a lab at Cedars Sinai in 
LA come to teach us how to do it. We did all of 10 stains at first. Of course 
it was all manual and so had to know what was going on with every step. I 
didn't use an automated stainer for the first 12 years that I did IHC, and at 
times was doing 150 slides a day manually.

Gradually I ended up doing half time in histology and learned cutting, special 
stains, muscle histochemistry, immunofluorescence for kidney cases. I decided 
to work on the HT exam since I was doing all that work anyway. We had a lab of 
four men - pretty rare, Imagine - and we started a study group to all take the 
test. We met after work a couple times a week for 6 months pretty  much 
memorizing the Sheehan book. We all took the HT and all but one passed. Later I 
passed the HTL as well.

After 11 years of that I moved on to a job in Saudi Arabia - and my wife and 
daughter went along. I managed the IHC and muscle lab at King Faisal Specialist 
Hospital in Riyadh. My wife was lucky enough to get a teaching position at the 
American School where our daughter was in 9th grade. That made all the 
difference in our life there because if she had not gotten a job I don't think 
we would have stayed there  5 years. She would have been stuck doing pretty 
much nothing. I moved on to managing the histology lab as  whole. Living in 
another country is a great experience, even if it is a totally different 
culture. It certainly changed our outlook on the world and I would not trade 
that experience for anything. We also did a lot of travelling during those 
years - being on "that" side of  world makes traveling there much easier!

Once we decided to leave Saudi I looked for a job back in the States and was 
lucky enough to land one at the Centers for Disease Control in Atlanta in their 
Infectious Disease Pathology division. I worked with 5 infectious disease 
pathology specialists and a dozen technologists from histotechs to EM techs, to 
microbiologists to molecular biologists. We worked on routine cases to 
world-wide outbreak cases. During the 5 years I was there we identified at 
least one novel human virus every year that caused outbreaks. And that was in 
addition to numerous cases of outbreaks of known diseases for which we received 
samples from all over the world. Probably the most notorious case was the 
anthrax attack after 9/11. Four of us histotechs manned the lab 24 hours a day, 
7 days a week for 6 weeks running IHC tests on endless samples while trying to 
get on top of that case. In the middle of it all the power went out to the 
facility and we had to work on generator power with temporary lighting set up 
in the lab and battery packs to keep the equipment running. After 9/11 and then 
anthrax 

[Histonet] GSH update

[Shirley A. Powell via Histonet]

Subject: GSH update


The Georgia Society for Histotechnology invites everyone to our Virtual Summit 
on May 22, 2021 beginning at 8:00 am until 4:30 pm.

This virtual meeting will be a great opportunity to get 6 CEU's through some 
excellent speakers for the 6 sessions.

Each session is $10 but if you choose all 6 the cost is $50.

We hope to do another in fall in order to stay committed to providing the 
needed education of histotechs.  Please see the program with the topics of the 
sessions below.  Also visit our website at 
https://georgiahistotech.org/gsh-spring-symposium/ for the program with 
abstracts, list of vendors and registration form.

Hope to see you there,

Ely Klar

GSH President


Georgia Society for Histotechnology
GSH 2021 SUMMIT
Saturday, May 22, 2021
**VIRTUAL**

Itinerary - Saturday, May 22, 2021
8:15am Welcome to the GSH Virtual Summit! - Opening Remarks
Ely Klar, MS, HTL(ASCP), Columbus State University, GSH President

8:30am Session I: Exploring Special Stains
Carl Sagasser, MBA, BS, HT(ASCP), AmeriPath

9:30am Session II: Her2 Dual ISH: Built For You. Built For Her.
Tammy Maury, HTL, Roche/Ventana Pathology Solutions Specialist

10:30am  Break I with Virtual Vendor "Exhibits"
Various Vendors - See Program for List of Vendors

11:00am  Session III: CINtec Plus: The Next Evolution in Cancer 
Screening
Tammy Maury, HTL, Roche/Ventana Pathology Solutions Specialist

12:00pm Session IV: The Veterinary Histology Mystic: Compare and 
Contrast
Shaele Litteral, HT(ASCP), Tifton Veterinary Diagnostic and Investigation 
Laboratory (TVDIL)

1:00pm Lunch

1:30pm Break II with Virtual Vendor "Exhibits"
Various Vendors - See Program for List of Vendors

2:00pm Session V: Performing IHC Procedure Properly
Joe Myers, MS, CT(ASCP)QIHC, BioCare Medical LLC

3:00pm Break III with Virtual Vendor "Exhibits"
Various Vendors - See Program for List of Vendors

3:30pm Session VI: Troubleshooting Immunohistochemical Staining 
Procedures
Joe Myers, MS, CT(ASCP)QIHC, BioCare Medical LLC

4:30pm Thank you and Closing Remarks
Ely Klar, MS, HTL(ASCP), Columbus State University, GSH President

*Note: Session access information will be provided to registered participants 
before the meeting date


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Re: [Histonet] Fume hood filter

[Shirley A. Powell via Histonet]

That website did not transfer right. 
www.pathologyserv.com
is the correct one

-Original Message-
From: Shirley A. Powell via Histonet  
Sent: Thursday, September 24, 2020 12:03 PM
To: Hannen, Valerie 
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Fume hood filter

Pathology Service, Inc, phone number is 1-866-398-9478 have these filters in 
different sizes.
https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.pathologyserv.com%2Fdata=02%7C01%7Cpowell_sa%40mercer.edu%7Ccb22de696fa24f4617fb08d860a3709c%7C4fb34d2889b247109bcc30824d17fc30%7C0%7C0%7C637365602375331091sdata=AfDrNObUZ7GOnLz4d%2FcdnCa9utZFs5sDHrZsEKla%2FI8%3Dreserved=0
 is their website.
Shirley Powell

-Original Message-
From: Hannen, Valerie via Histonet  
Sent: Thursday, September 24, 2020 11:57 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fume hood filter

Hi everyone.. hope all are well!!

I have a slight dilemma...  I have a "Fume-Gard" (old) fume hood and my current 
vendor is out and will not be restocking.  Is there anyone else out there that 
is using this fume hood for coverslipping??  The dimensions are as follows:  H: 
5.8 inches, D: 1.5 inches and W: 11.2.

Can anyone steer me to a vendor who might have such filters with these 
dimensions?

Thanks !!

Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com<mailto:valerie.han...@parrishmed.com>
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Re: [Histonet] Fume hood filter

[Shirley A. Powell via Histonet]

Pathology Service, Inc, phone number is 1-866-398-9478 have these filters in 
different sizes.
www.PathologyServ.com is their website.
Shirley Powell

-Original Message-
From: Hannen, Valerie via Histonet  
Sent: Thursday, September 24, 2020 11:57 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fume hood filter

Hi everyone.. hope all are well!!

I have a slight dilemma...  I have a "Fume-Gard" (old) fume hood and my current 
vendor is out and will not be restocking.  Is there anyone else out there that 
is using this fume hood for coverslipping??  The dimensions are as follows:  H: 
5.8 inches, D: 1.5 inches and W: 11.2.

Can anyone steer me to a vendor who might have such filters with these 
dimensions?

Thanks !!

Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com<mailto:valerie.han...@parrishmed.com>
https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.parrishmed.com%2Fdata=02%7C01%7Cpowell_sa%40mercer.edu%7C7a18833e0e3546e3818708d860a29cd9%7C4fb34d2889b247109bcc30824d17fc30%7C0%7C0%7C637365598820886967sdata=7Jb%2BCB8xVOh3U4VAbtweoVEOfhWlYI6MH7a9xL47nrs%3Dreserved=0

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[Histonet] Freezing Sprays in Cryostats

[Shirley A. Powell via Histonet]

I agree with not using a spray in cryostats.  We had to get tested a few times 
in the hospital for TB after discovering the case was positive after the fact, 
scary.  This is the same as flushing the toilet with the seat open.  I know 
that sounds crazy but here is just one article of many online that explains the 
aerosol effect.  
https://www.realtor.com/advice/home-improvement/flushing-the-toilet-with-the-lid-up-what-happens/
 

Please don't send me criticism for posting this, it is real. Read the articles. 
It is a good reason to stop using sprays in the cryostat.  


Shirley Powell, HTL (ASCP)
Technical Director Histology Curricular Support Lab
Pathology
Mercer University School of Medicine
1550 College Street | Macon, GA | 31207
O:478-301-2374| F:478-301-5489
medicine.mercer.edu.







-Original Message-
From: Bob Richmond via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, September 26, 2019 12:05 PM
To: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Freezing Sprays in Cryostats

Freezing sprays for frozen sections in cryostats are deplorable, but try and 
get pathologists to give them up. I think that's probably why the CAP has been 
reluctant to ban them.

During my career in pathology I saw more than one case where frozen sections 
were inadvertently cut on tuberculous tissue. Tuberculosis is a disease that 
sneaks up on you. In Baltimore (a hotbed of the disease) around 1970 when we 
did this, we'd put the cryostat (one of the basic Damon IEC models we had then) 
out of action until somebody (usually the chief
resident) could bring it up to room temperature, scrub it out with alcohol, 
lubricate it, and plug it back in to cool down.

The heat extractors are usually all you need. Liquid nitrogen is not a very 
satisfactory freezing medium. It's better to submerge a freezing medium liquid 
into liquid nitrogen (which will eventually freeze it solid, however). Shandon 
used to market a freezing container called a Histobath - is any equivalent 
product available today? It held the right temperature not to freeze the liquid 
medium solid.

People usually use acetone or isopentane (2-methylbutane) as the freezing 
medium, both highly flammable. Better is:
3M™ Novec™ Engineered Fluid HFE-7100
This product belongs to a class of fluorocarbons called "segregated 
hydrofluoroethers (HFE's)"
According to various MSDS, HFE-7100 is
methyl nonafluoroisobutyl ether
C4F9-O-CH3

But try to substitute any such thing for freezing spray, and be prepared for a 
hissy-fit from your senior pathologist.

I'm glad the CDC weighed in on this. I do remind them that the decision to do a 
frozen section is between the surgeon and the pathologist.

Bob Richmond
Samurai Pathologist
Maryville TN
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[Histonet] The Georgia Society For Histotechnology 2019 Histopalooza meeting

[Shirley A. Powell via Histonet]

My First message was too large, here is another try.
Shirley


Hi Everyone,
The Georgia Society for Histotechnology has a new website.  Visit the site 
here:http://georgiahistotech.org/gsh-symposium/  You may download the 
registration forms to print and mail with a check or pay through PayPal with 
credit card.  The hotel reservation information is on the same page.

Come to Georgia coast for a great meeting.  There is only a week and a half 
left, so make plans today.
The program at a glance is below to make your choices of workshops.

Shirley Powell
GSH Registrar



Georgia Society for Histotechnology

HISTOPALOOZA! May 3-5, 2019 PROGRAM

Hotel Tybee, Tybee Island, Ga







Friday

ROOM A 8:30 am -5:00 pm   ROOM B 3:30 
pm -4:30 pm

#1: A Guide to passing the HT/HTL Certification #2: The Cost of 
Reprocessing

  Carl Sagasser, MBA, BS, HT (ASCP)   6 hours  Skip Brown, HT 
(ASCP) - 1 hour



BREAK: 10:00-10:30 / LUNCH: 12:00 - 1:30 / Break: 3:00 - 3:30


VENDOR RECEPTION: 7-10 p.m. in Vendor Exhibit Hall
Saturday
ROOM A 8:30 am-12:00 am  ROOM B 8:30 
am- 9:30 am
#3 Leadership:The Pinnacle of Management &#4: The benefits of automation or 
why to
Supervision - Skip Brown, HT(ASCP)- 3 hours  move away 
from dip and dunk systems


Dan Hopkins, Roche - 1 hour



BREAK: 10:00-10:30 in Vendor Exhibit Hall




 ROOM B 10:30 am- 11:30 am


 #5 Equipment Maintenance


  Matt Lanford, TechOne- 1 hour



AWARDS LUNCHEON - 12:00-1:30



ROOM A 1:30 pm- 5:00 pm  ROOM B 1:30 pm 
- 3:00 pm

#6 Introduction to IHC#7 
How to Prepare Macro and Not So
 Kimya Jones HT, QIHC (ASCP)- 3 hours   Macro Sections

 Shirley Powell, HTL, HT(ASCP)- 1.5 hours



BREAK: 3:00-3:30 in Vendor - in the Exhibit Hall




 ROOM B 3:30 pm- 5:00 pm

 #8 Taking Control of Your IHC Controls

  Steve Westra, StatLab- 1.5 hours



General GSH Membership Meeting at 5:00 - ROOM B



Sunday
Room A 8:30 am- 12:00 pm ROOM B 8:30 
am- 9:30 am

#9: Human Tissues: Histological Identification of #10 Digital 
Pathology: What it is, how and
  the Major Tissue Types --   
where it is used, and why you should
  Ely Klar, MS, HTL (ASCP) - 3 hours   care

Aigars Brandts, PhD, Roche- 1 hour


BREAK: 10:00-10:30 in Vendor Exhibit Hall

NOTE: Vendor exhibits close at noon





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[Histonet] Slide disposal

[Shirley A. Powell via Histonet]

Hi Histonetters, mostly in the southeastern states, i.e. Georgia or South 
Carolina.

I need to dispose of some years of slides that has patient identifying info on 
them, are there any companies that grind slides in the state of Georgia?  I 
know there are companies that sell machines for this but I don't have the 
budget nor the volume for buying one.

Thanks for your assistance in advance.

Shirley A Powell, HTL(ASCP)
Technical Director Histology Curricular Lab
Mercer University
Pathology Department
1501 Mercer University Drive.
Macon, GA 31207-0001
PH:  478-301-2374
FX:  478-301-5489
Email: powell...@mercer.edu<mailto:powell...@mercer.edu>



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Re: [Histonet] Disposal of Bouin's Solution

[Shirley A. Powell via Histonet]

I agree it is very dangerous.  When I discovered a dried bottle of picric acid 
someone had donated to the school right after I first came to the university.  
I had to call the fire department and the bomb squad came to get it.  They took 
it to the firing range and blew a huge sized whole in the ground, this was a 
small bottle of picric acid.  Of course we made the local news, but get rid of 
it if possible or keep it under water as Fred says. 
Shirley 

-Original Message-
From: Monson, Frederick via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, May 12, 2017 2:16 PM
To: Histonet@lists.utsouthwestern.edu
Cc: Bob Richmond 
Subject: Re: [Histonet] Disposal of Bouin's Solution

https://oag.ca.gov/sites/all/files/agweb/pdfs/cci/safety/picric.pdf

Two hazards:  formaldehyde and picric acid (see attached).
While I agree with Dr. Richmond, changing protocols is not easily done.
So.  You may search for appropriate oxidizers of aldehydes.  OR!!!  You may 
take the reasonable path by sending it off periodically to a company that will 
cost enough to raise the use of the stuff to serious conversation.  

Picric acid - 
  since I used it from the late 1950's - the end of our 
"Dark Ages"[???] - 
into the mid 1970's -the age when 
students became consumers  and knowing what was on the test was paramount -  
  IS really 
hazardous - when it is dry.  [So, I always kept it covered with water 
(saturated in local conditions.] Picric acid is the reason that you should 
either stop using Bouin's or waste it (wet!!) to a competent waste-handling 
company.

Cheers,

Fred Monson (5 weeks to the oblivion called 'retirement.'

-Original Message-
From: Bob Richmond via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, May 11, 2017 6:39 PM
To: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Disposal of Bouin's Solution

Lori Jones, CT(ASCP), Pathology Supervisor, Ingalls Memorial Hospital asks:

>>We use Bouin's Solution in our pathology department and are currently
disposing of it by neutralizing it with Vytac for formalin. I can't find 
supporting documentation that this is the proper way to dispose of it. I'd like 
to know if and how other institutions are neutralizing Bouin's solution.<<

The best way to dispose of Bouin's fixative is by not buying it at all.
What do you use it for? There are substitutes for it for most purposes.

Bob Richmond
Samurai Pathologist
Maryville TN
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[Histonet] I have a job question

[Shirley A. Powell via Histonet]

Below is what is found when subscribing to histonet at 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet.  The two doctors who 
run histonet are highlighted below in yellow.  They would be the ones who make 
the rules and those questions should be directed to them. There are no hard and 
fast rules here to define who can post and what can be posted.

Sometimes subscribers think they know the rules of the listserve, but need to 
ask the ones who are in charge first what they are.  Maybe they would be kind 
enough to share them with us.






Histonet -- For the exchange of information pertaining to histotechnology and 
related fields




About Histonet

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WHO SHOULD SUBSCRIBE?

Anyone interested in research or clinical applications of histology, 
immunohistochemistry, in-situ hybridization pathology, and electron microscopy 
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from major urban centers and obscure remote locales, university researchers, 
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WHO RUNS HISTONET?
The list is run by Anita Sengupta M.D. and Linda R. Margraf, M.D. using 
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School in Dallas, Texas. If you have any questions or problems with Histonet 
please contact Linda Margraf at lindamarg...@gmail.com

To see the collection of prior postings to the list, visit the Histonet 
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To post a message to all the list members, send email to 
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You can subscribe to the list, or change your existing subscription, in the 
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Re: [Histonet] Tissue processing question

[Shirley A. Powell via Histonet]

Not advertising but I do a lot of research on tiny pieces of tissue and have 
found the perfect cassette from Cancer Diagnostics.  It is the Vortex 
corner-less ones seen here.
http://cancerdiagnostics.com/index.php/cassettes-accessories/microbiopsy-cassettes-and-specialty-cassettes/vortex-biopsy-cassette.html

They have two sizes in these.  I have to process specimens the size of a gnat's 
eye and they do not get lost.  No corners to deal with.

Shirley





-Original Message-
From: Caroline Miller via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, January 29, 2016 1:36 PM
To: Walter Benton 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue processing question

I really like this type:
https://www.fishersci.com/shop/products/starplex-scientific-histoplex-tissue-cassettes-micromesh-chamber-8/p-2782584

(although I buy them from mastertech, but they seem to have dissapeared from 
their website)

They are great for both large tissues, and also biopsies. A long time ago when 
I worked in a clinical lab we used the tissue paper and I found that if 
everything was not heated just right the biopsies would stick and things like 
currettes were hard to scrape up from there, I always thought I was doing the 
tissue damage

yours
mills

On Fri, Jan 29, 2016 at 10:06 AM, Walter Benton via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> We use hair wrapping paper used for perms. It is the same paper called
> "biopsy wraps," but at a significant price reduction. You can buy a
> variety of sizes and the wraps do not cause artifacts and are porous
> enough for ample solution penetration. Biopsy paper comes in blue and
> other colors, but the hair wraps only come in white. Our overall
> experience with them has been great.
>
> Let me know if you need any other information.
>
>
> Walter Benton HT(ASCP)QIHC
> Lab Operations Manager
> Chesapeake Urology Associates
> 806 Landmark Drive, Suite 127
> Glen Burnie, MD 21061
> 443-471-5850 (Direct)
> 410-768-5961 (Lab)
> 410-768-5965 (Fax)
> Chesapeakeurology.com
>
> Voted a Best Place to Work by
> Baltimore and Modern Healthcare
> Magazines.
>
>
>
> -Original Message-
> From: Charles Riley via Histonet
> [mailto:histonet@lists.utsouthwestern.edu
> ]
> Sent: Friday, January 29, 2016 12:43 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Tissue processing question
>
> Hello all,
>
>  I was wondering what everyone uses to secure biopsy and scant tissues
> through processing. Also what would you recommend placing breast cores
> in for processing. Having an argument with grossing staff and
> pathologist about whether to use sponges, tissue paper, or something
> else. Looking for the best option that will allow for reagents to
> penetrate tissue and not leave any artifact
>
> --
>
> Charles Riley HT(ASCP)CM
>
> Histopathology Coordinator/ Mohs
>
> Doctors Pathology Services, Dover DE
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--
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
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[Histonet] Mollusk, gastropods

[Shirley A. Powell via Histonet]

Hello to the wonderful world of histonet.

I have been asked to section mollusks/gastropods for research.  Let me just say 
I have no experience in this area, but always willing to expand my knowledge 
and expertise in histology.

The source of the specimens say that the critters were dead before putting them 
in formalin, they died for reasons unknown and have no normal ones to compare 
to the diseased ones.
I would like to hear from anyone who has sectioned snails and I would like to 
see photos of sections on the mollusks that were viable before going into the 
formalin.  The ones I cut sectioned well but not sure what they are supposed to 
look like when healthy and fixed properly.  We have googled and looked in books 
without success. So it was time to Ask Histonet.

Please respond to me privately if you have experience with sectioning mollusks 
and are willing to share photos and information.
powell...@mercer.edumailto:powell...@mercer.edu

Thanks in advance.

Shirley Powell

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Re: [Histonet] OJT Histotechs/Training

[Shirley A. Powell]

Oh where is the LIKE button Pam?  Thanks, and that is the reason NSH and 
state societies were begun, to educate the public about our existence and that 
we are very valuable to them.  

Shirley, soon to be 53 years in the shadows.  


-Original Message-
From: Pam Marcum [mailto:mucra...@comcast.net] 
Sent: Thursday, May 14, 2015 12:18 PM
To: Lisa Roy
Cc: Histonet; Michael Dessoye
Subject: Re: [Histonet] OJT Histotechs/Training

I understand and agree with everything being said and feel we do need more 
education in getting your registry, as Histology is changing and growing.  We 
need to be prepared to grow with it, much as we did when IHC first came into 
Histology and many thought it would go to the MTs.   
  
The one thing that has not changed in the 50 years I have done Histology is the 
fact that no one outside of AP knows what a Histologist is or what we do.  (I'm 
tried of being asked Oh what kind of history is that?)  Until we change that 
and get more information about the field and advantages we will still be in the 
straights we are in now.  No one joining because so few people even know what 
we do or that there is an opportunity here.  If you don't know what Histology 
is why would you even look at the field.  I know about and have done school 
visits, career days etc; and those are not enough.  
  
We need a spokesperson or celebrity who has needed our services and not even 
known we, Histology, were the ones who did the slides their wonderful doctors 
used to save their lives.  This person or persons needs to speak loud and 
strong the way Robin Roberts has done on TV for her doctors and help. However; 
Histology was neven mentioned in those gratis moments. I have only known one 
person in NSH who suggested this and no one listened.  If they can't see you or 
know you - you don't exist.  Can we all take off the blinders and look at what 
we need in publicity and stop waiting for NSH and ASCP to do it.   Then we can 
offer these possible future HTs and HTLs something, like being recognized as 
full laboratory professionals and a higher level of lab aide. 
  
Just my thoughts (for many years and spoken often) 
  
Pam Marcum 

- Original Message -

From: Lisa Roy ro...@labcorp.com 
To: Michael Dessoye mjdess...@commonwealthhealth.net, Histonet 
histonet@lists.utsouthwestern.edu 
Sent: Thursday, May 14, 2015 7:55:19 AM 
Subject: Re: [Histonet] OJT Histotechs/Training 

I currently have 3 open tech positions and don't have any qualified applicants 
applying for the job.  I have recently taken a lab aide that showed interest 
and aptitude and began OJT.  With less than 30 schools in the country actually 
teaching histology, this is one day going to be the way.  Already having a 
bachelors in biology, my aide qualifies to sit for the ASCP exam once he has 
completed one full year of tech work and has a pathologist willing to review 
his work and sign off on the ASCP paperwork.  Without going through a 
traditional program, one must have an associates or bachelor's degree with a  
certain amount of Chemistry and Science credits.  As far as the training, I 
started with embedding and moved on from there to cutting and then special 
staining.  All along way, working on troubleshooting and documenting 
EVERYTHING.  Some places will hire someone with only a high school diploma as 
long as they have previous HT experience.  I think the specifics of what each 
institution would deem a qualified trainee will vary from place to place.  
Smaller hospitals or labs may be okay training someone with aptitude that 
doesn't necessarily fit the ASCP exam qualifications, but large corporations 
might really insist that the trainee be certifiable at some point. 

Frankly, I think taking someone that shows an interest and has the knowledge to 
be a great tech is better than hiring someone that you may not know what you 
are getting.  Doing OJT ensures that you are teaching the candidate exactly how 
you want things done and not having to accept the bad habits of someone that 
has been doing it a long time and set in their own ways. 

Good luck 
Lisa     

-Original Message- 
From: Dessoye, Michael [mailto:mjdess...@commonwealthhealth.net] 
Sent: Thursday, May 14, 2015 7:44 AM 
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] OJT Histotechs/Training 

Hello Histonet, 

I'm curious how people are dealing with on-the-job-trained histotechs.  Many 
people are seeing a shortage in techs, and in my opinion OJT will become more 
common than it already is.  Does anyone have an 'official' training program?  
Requirements to pass the exam?  Qualifications to be able to be trained 
on-the-job?  I'd like to consider having some kind of plan in place when I 
don't have an HT/HTL applicant but have folks who, if they get the experience, 
are otherwise qualified to sit for the exam.  If anyone has a similar situation 
or experience to share I would appreciate it! 

Thanks, 
Mike 

Michael J. 

Re: [Histonet] Tissue transfer method:

[Shirley A. Powell]

With Mount Quick you can also decolorize the section and do a different stain 
on them.  I have even performed IHC on these transferred sections when needed.

Shirley Powell

-Original Message-
From: James Watson [mailto:jwat...@gnf.org] 
Sent: Friday, May 01, 2015 4:21 PM
To: 'Jb'; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue transfer method:

Slide Repair

1.  Soak coverslip off of slide in xylene.
2.  Piece slide back together.
3.  Apply Mount-Quick to slide, covering tissue. Allow to dry overnight.
4.  Place slide in cold water in refrigerator overnight.
5.  Carefully peal the Mount-Quick off the slide.  The tissue should come 
up with the Mount-Quick, if it does not stop pealing up the Mount-Quick and 
place back into the water overnight again.
6.  Carefully trim excess Mount-Quick from areas where tissue is not 
present. Leave at least ¼ inch of Mount-Quick around the tissue
7.  On a new slide that is wet with water, place the removed Mount-Quick 
and tissue in the center of the slide (make sure the tissue is next to the 
slide), removing as many air bubbles from under the Mount-Quick as possible.
8.  Cover the slide with a wet piece of filter paper then place a blank 
slide on top.  If you have multiple slides to repair then they can be stacked.  
Apply weight to the top of this and let stand overnight.
9.  Soak the dry slide in Xylene until the Mount-Quick is removed and 
coverslip as usual.

James Watson HT  ASCP
GNF  Genomics Institute of the Novartis Research Foundation Scientific 
Technical Leader II, Histology Tel    858-332-4647 Fax   858-812-1915 
jwat...@gnf.org

-Original Message-
From: Jb [mailto:craiga...@gmail.com]
Sent: Friday, May 01, 2015 1:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue transfer method:

Does anyone have a procedure that they can share on tissue transfer? One 
stained slide to another?

Thank you for your help.

Sincerely,

Craig

Sent from my iPhone
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RE: [Histonet] BS in Histotechnology

[Shirley A. Powell]

Thank you Dr. Stedman.  

I appreciate your response.  I have been fortunate to work with mostly 
respectful and appreciative pathologists in my 53 (in July) years as a 
histotechnologist. But have met some who think more of themselves than they 
should.  

One more thing, there are thousands of histotechs grossing now, a task that was 
once only a pathologist's job.  So I wonder if the offenders think that a 
monkey can do their job?  Just a thought.  

Thank you again for being a professionial.

Shirley Powell

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stedman, Nancy
Sent: Tuesday, March 24, 2015 5:22 PM
To: Mark Turner; Paula Sicurello; Michael Ann Jones
Cc: histonet@lists.utsouthwestern.edu; Timothy Morken; Jennifer MacDonald; 
Marcum, Pamela A
Subject: RE: [Histonet] BS in Histotechnology

As a pathologist I'd like to apologize for all the pathologists who have made 
comments like this.. forget trained monkeys and dogs, most (all?) pathologists 
cannot cut slides either, at least not slides they'd want to try to read.   I 
know I can't.   

-Nancy Stedman 




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Turner
Sent: Tuesday, March 24, 2015 4:26 PM
To: Paula Sicurello; Michael Ann Jones
Cc: histonet@lists.utsouthwestern.edu; Timothy Morken; Jennifer MacDonald; 
Marcum, Pamela A
Subject: RE: [Histonet] BS in Histotechnology

I once worked with a Pathologist who said she was in a group meeting of other 
pathologists when one of them blurted out that a trained monkey could cut 
slides.  My pathologist, having had the opportunity to review some cases from 
the offender's laboratory, promptly replied Yes, and with the quality of your 
slides it looks like you did just that.  She shut down the other pathologist 
really quickly, and as far as I know, we never received another case to review 
from him.  My pathologist was not about to let that kind of arrogance stand.  
She was one of the best bosses I ever had!

Mark Turner,  Ph.D., HT(ASCP)QIHC
Manager, Histology/IHC
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello
Sent: Tuesday, March 24, 2015 3:47 PM
To: Michael Ann Jones
Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald; Marcum, Pamela A; 
Timothy Morken
Subject: Re: [Histonet] BS in Histotechnology

I've had more than one pathologist tell me a monkey could do my job.
Though one of them said it with a smile and added a very highly skilled and 
well trained monkey, he was one of the few who knew better.

How many of us monkeys have trained the whining and complaining residents how 
to do things correctly?

Paula

On Tue, Mar 24, 2015 at 12:29 PM, Michael Ann Jones mjo...@metropath.com
wrote:

 OMG Pam~ our pathologist said the exact same thing to us when we 
 started our Grossing training.
 Sheesh. . .
 Michael Ann




 On 3/24/15, 11:52 AM, Marcum, Pamela A pamar...@uams.edu wrote:

 That was nicer than the pathologist who told me years ago, any 
 monkey could be trained to do my job.  I basically did not take the 
 job I was interviewing for at the time.  At least the next interview 
 went a lot better and I did take the job.
 
 Pam
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
 Sanders, Jeanine (CDC/OID/NCEZID)
 Sent: Tuesday, March 24, 2015 12:30 PM
 To: Sue; Timothy Morken
 Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald
 Subject: RE: [Histonet] BS in Histotechnology
 
 I agree, BUTas long as many pathologists think you can 
 teach any trained dog how to section histology will never have the 
 recognition those of us that have studied and trained deserve.
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
 Sent: Tuesday, March 24, 2015 12:59 PM
 To: Timothy Morken
 Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald
 Subject: Re: [Histonet] BS in Histotechnology
 
 This is a fight that we continue to have with hospital administration.
 In my opinion histologists are just as important and needed as MT.  
 Even though there is an increase in automation in pathology the hands 
 on of a histologists is most important.  The fact that hospital still 
 consider a lower entry job is the reason there are not more of us.
 It is quite frustrating.
 
 Sue
 TJUH
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RE: [Histonet] sta on

[Shirley A. Powell]

I use it.  Back when I was doing my IHC reference lab, I had certain tissues 
that would not stay on with charged slides.  These were bloody, crunchy and 
brittle tissues mostly.  When they washed, I used Sta-On and they worked great. 
 No washing.  After my institution closed the IHC part of my lab in 2002, I 
decided to use Sta-On and not charged slides which was more cost effective.  I 
still use it today.  Very seldom do I lose any sections.  

Shirley Powell
MUSM


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita
Sent: Wednesday, March 25, 2015 12:57 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] sta on

how many people use sta on in their waterbath?   we have not for years, but 
have a new person who wants me to get some, she is having trouble with the 
ribbons floating away.  

 

thanks for your input,

anita dudley

providence hospital

mobile, al
  
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[Histonet] And other crazy stuff.

[Shirley A. Powell]

Orangutan testicle macro section and alligator jawbones, not my best work, very 
humbling, after 52 years in the business.
Shirley 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Tuesday, January 06, 2015 2:24 PM
To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet@Lists. Edu
Subject: And other crazy stuff. RE: [Histonet] cutting honey bees

You crazy research people...OK, so what is the craziest thing you ever had to 
cut, or were asked to cut?

For me, not too bad, but embedding for EM and sectioning a single oocyte that 
was nearly microscopic. I'll just say it took a LOT of thick sections too face 
down to it without actually cutting through it.


Open the floodgates

Tim Morken

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Tuesday, January 06, 2015 11:13 AM
To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu
Subject: RE: [Histonet] cutting honey bees

for the whole bee I probably would process and embed it in glycol methacrylate 
(gma) it is much harder and would give better sections, we have done zebra fish 
and several other harder tissues including calcified bone in GMA.

Cheers,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



 From: r...@psu.edu
 To: classic...@gmail.com; histonet@lists.utsouthwestern.edu
 Date: Sat, 3 Jan 2015 23:15:33 +
 Subject: RE: [Histonet] cutting honey bees
 CC: 
 
 I sectioned and stained honey bee and yellow jacket stingers years ago.  They 
 wanted to show the difference between the stingers.  I wasn't sure what to do 
 so I processed and handled like everything else.  I was able to get some good 
 sections.  I put 6 stingers in each block and cut several sections figuring 
 there should be at least one good stinger in each block and it worked.
 Roberta Horner
 Penn State University
 Animal Diagnostic Lab
 
 From: histonet-boun...@lists.utsouthwestern.edu
 [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg 
 [classic...@gmail.com]
 Sent: Saturday, January 03, 2015 6:08 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] cutting honey bees
 
 Has anyone had experience embedding and cutting honey bees. I am sure 
 there are some issues with the harder exoskeleton. Would that have to 
 be dissected away first. I am considering helping a student with a 
 science fair project on bees.
 
 Douglas Gregg
 Veterianary pathologist
 
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[Histonet] RE: Prostate Needle Bx

[Shirley A. Powell]

I have discovered a wonderful cassette from Cancer Diagnostics, it is called 
the Autocassette and has an oval concave cavity with no corners for small 
pieces of tissue to get caught in and the solutions pass through it very 
nicely.  I use it for small research samples, like shrimp gills similar in size 
to the prostate biopsies, and I lose nothing.  I use the yellow ones, VB1005 
but they have other colors and also some with smaller grid.  Highly recommend 
them.  

Shirley

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Wednesday, December 03, 2014 11:49 AM
To: Histonet Post (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Prostate Needle Bx

How do others process prostate needle bx's - what kind of cassettes do you use? 
 Do you use sponges?  Do you wrap the tissue in lens paper?  Do you process 
them on a biopsy (short) run?  Our bx's always seem to be less than optimal, 
but we do not have problems with other small bx's.

Laurie Colbert
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RE: [Histonet] white plastic scrapers

[Shirley A. Powell]

Metal scrapers may remove some of the ceramic coatings on embedding centers, 
they do have plastic putty knives at Lowes or Home Depot or where ever you get 
hardware.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian
Sent: Tuesday, October 14, 2014 8:38 AM
To: Histonet@lists.utsouthwestern.edu; bmolin...@texasheart.org
Subject: RE: [Histonet] white plastic scrapers

You know what works better? A small, metal drywall taping/mud knife-maybe 3-4 
inches. They're more flexible than the plastic scrapers, and I think they 
actually pick up more paraffin per pass. You can pick these up at any hardware 
store.

Thanks,

Brian Cooper, HT (ASCP)
Histology Supervisor,
Path  Lab Medicine
Children's Hospital, Los Angeles

Sent from my Galaxy S3, so please forgive any weird typos . . .


-Original Message-
From: Betsy Molinari [bmolin...@texasheart.org]
Received: Tuesday, 14 Oct 2014, 5:08AM
To: Histonet@lists.utsouthwestern.edu [Histonet@lists.utsouthwestern.edu]
Subject: [Histonet] white plastic scrapers

Hi all,
Where can I purchase the whit plastic scrapers used to scrape the paraffin off 
embedding centers and other surfaces. I believe ours went out with the trash.
Thanks.

Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
Houston,TX 77030
832-355-6524 (lab)
832-355-6812 (fax)



http://www.texasheart.org



Betsy Molinari
Senior Histology Research Technician
832-355-6524 | bmolin...@texasheart.orgmailto:bmolin...@texasheart.org | 
www.texasheart.orghttp://www.texasheart.org



6770 Bertner Ave., MC 1-283, Houston, TX 77030



[Texas Heart 
Institute]https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html[THI
 News] [THI on Facebook] http://www.facebook.com/Texas.Heart.Institute  [THI 
on Flicker] http://www.flickr.com/photos/texasheart/sets/  [THI on Google] 
https://plus.google.com/u/0/118043615690351997044/posts   [THI on Pinterest] 
http://pinterest.com/texasheartinst/  [THI on Twitter] 
http://twitter.com/Texas_Heart  [THI on You Tube] 
http://www.youtube.com/TexasHeartInstitute


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the sole use of the intended recipient(s) and may contain confidential or 
legally privileged information. Any unauthorized review, use, disclosure or 
distribution is prohibited. If you are not the intended recipient, please 
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[Histonet] RE: biopsy bags for processing - alternatives

[Shirley A. Powell]

Hi Tim and all, 

I recently did a search for cassettes that I can process tiny, and I mean tiny 
(we are talking gnat's eyes, not really), specimens and found some cassettes 
that were perfect for them and one for a little larger, but still tiny.  The 
came from Cancer Diagnostics, no I do not get a kick back.  They are called 
AutoSette Cassettes (Vortex Cornerless Biopsy Cassettes) and are concave with 
NO corners for the tiny pieces to get caught in.  I deal with a lot of small 
and unique tissues/animals and these work perfectly.  Repeating myself, but the 
VM1007 ones are for the really extra tiny specimens and the VM1005 are for the 
little larger specimens.  You can get them in colors to differentiate types of 
sources.  You can see them here.  
http://www.cancerdiagnostics.com/CDI_Products.aspx?pid=153

I must say I have not lost anything yet. 


Shirley

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Wednesday, August 13, 2014 11:24 AM
To: Histonet
Subject: [Histonet] biopsy bags for processing - alternatives

All knowing Histonet,

Our grossing staff uses nylon biopsy bags to enclose some biopsy specimens. 
The embedding staff find them troublesome because when they pull the bags open 
they tend to pop open and throw the tissue off in all directions. They have 
to be very careful opening these. Is there another bag made of some other 
material that is less prone to this problem?

For various reasons some of these samples can't be put on sponges. They do wrap 
some in flat biopsy paper, but not others. It seems to be a grossing personal 
preference more than anything else.

Thanks for any and all info!

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC 
San Francisco Medical Center Box 1656
505 Parnassus Ave
San Francisco, CA 94143
USA

415.514-6042  (office)
tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org


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[Histonet] RE: biopsy bags for processing - alternatives

[Shirley A. Powell]

Correction the number is VB1005 but you can get all the numbers from the 
website. http://www.cancerdiagnostics.com/CDI_Products.aspx?pid=153


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. 
Powell
Sent: Wednesday, August 13, 2014 11:50 AM
To: Morken, Timothy; Histonet
Subject: [Histonet] RE: biopsy bags for processing - alternatives

Hi Tim and all, 

I recently did a search for cassettes that I can process tiny, and I mean tiny 
(we are talking gnat's eyes, not really), specimens and found some cassettes 
that were perfect for them and one for a little larger, but still tiny.  The 
came from Cancer Diagnostics, no I do not get a kick back.  They are called 
AutoSette Cassettes (Vortex Cornerless Biopsy Cassettes) and are concave with 
NO corners for the tiny pieces to get caught in.  I deal with a lot of small 
and unique tissues/animals and these work perfectly.  Repeating myself, but the 
VM1007 ones are for the really extra tiny specimens and the VM1005 are for the 
little larger specimens.  You can get them in colors to differentiate types of 
sources.  You can see them here.   

http://www.cancerdiagnostics.com/CDI_Products.aspx?pid=153



I must say I have not lost anything yet. 


Shirley

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Wednesday, August 13, 2014 11:24 AM
To: Histonet
Subject: [Histonet] biopsy bags for processing - alternatives

All knowing Histonet,

Our grossing staff uses nylon biopsy bags to enclose some biopsy specimens. 
The embedding staff find them troublesome because when they pull the bags open 
they tend to pop open and throw the tissue off in all directions. They have 
to be very careful opening these. Is there another bag made of some other 
material that is less prone to this problem?

For various reasons some of these samples can't be put on sponges. They do wrap 
some in flat biopsy paper, but not others. It seems to be a grossing personal 
preference more than anything else.

Thanks for any and all info!

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC 
San Francisco Medical Center Box 1656
505 Parnassus Ave
San Francisco, CA 94143
USA

415.514-6042  (office)
tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org


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RE: [Histonet] On the lighter side...

[Shirley A. Powell]

I agree. :)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire
Sent: Monday, August 11, 2014 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...


Old histologists never die, they are just well fixed...
Claire

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Michael Ann Jones 
[mjo...@metropath.com]
Sent: Monday, August 11, 2014 9:07 AM
To: Edwards, Richard; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] On the lighter side...

25 years, (what¹s that in micron¹s?²) Bernice, you are too funny!!
(lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) 
Histology Manager Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 8/11/14, 5:17 AM, Edwards, Richard r...@leicester.ac.uk wrote:

Sniffed my  first formalin and  saw  first post-mortem November 1965.

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RE: [Histonet] On the lighter side...

[Shirley A. Powell]

Yes, what Tim said, stay active.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Friday, August 08, 2014 12:45 PM
To: 'Beth Brinegar'; Elizabeth Chlipala
Cc: histonet@lists.utsouthwestern.edu; Cartun, Richard
Subject: RE: [Histonet] On the lighter side...

Yah! A new one!!! Stay on Histonet, go to meetings and stay interested- the 
burnout prevention!

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC 
San Francisco Medical Center San Francisco, CA

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar
Sent: Friday, August 08, 2014 8:01 AM
To: Elizabeth Chlipala
Cc: histonet@lists.utsouthwestern.edu; Cartun, Richard
Subject: Re: [Histonet] On the lighter side...

3 years in histology, 2 years registered!

Beth Brinegar HTL (ASCP)
Anatomic Pathology Supervisor
Mercy Medical Center
Cedar Rapids, IA 52403

Beth Brinegar HTL(ASCP)
Anatomic Pathology Supervisor
Mercy Medical Center
Cedar Rapids, IA 52403


On Fri, Aug 8, 2014 at 9:53 AM, Elizabeth Chlipala l...@premierlab.com
wrote:

 Too funny Richard - you would pass with flying colors

 For me its 31 years - HTL-930

 I have really been blessed, I love my job and I have really enjoyed my 
 career.  Every day there is something new to learn or to work on, for 
 example the lab is putting the final touches on a poster that will be 
 displayed at NSH this year, working on that has been exciting and a 
 lot of fun too.

 Liz

 Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO 
 Box 18592 Boulder, CO 80308
 (303) 682-3949 office
 (303) 881-0763 cell
 (303) 682-9060 fax
 l...@premierlab.com

 Ship to address:

 Premier Laboratory, LLC
 1567 Skyway Drive, Unit E
 Longmont, CO 80504
 
 From: histonet-boun...@lists.utsouthwestern.edu [ 
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, 
 Richard [ richard.car...@hhchealth.org]
 Sent: Friday, August 08, 2014 8:32 AM
 To: Douglas Porter; histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] On the lighter side...

 36 years; however, not registered.   Hopefully, I can take the QIHC
 someday and pass.

 Richard

 Richard W. Cartun, MS, PhD
 Director, Histology  Immunopathology
 Director, Biospecimen Collection Programs Assistant Director, Anatomic 
 Pathology Hartford Hospital
 80 Seymour Street
 Hartford, CT  06102
 (860) 972-1596 Office
 (860) 545-2204 Fax
 richard.car...@hhchealth.org


 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter
 Sent: Thursday, August 07, 2014 2:39 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] On the lighter side...

 How long have you been a registered histotech?  36 years here.  You???



 Douglas A. Porter, HT (ASCP)
 Grossing Technician
 IT Coordinator

 Cancer Registrar


 CAP-Lab, PLC
 2508 South Cedar Street
 Lansing, MI 48910-3138

 517-372-5520 (phone)
 517-372-5540 (fax)

  mailto:doug.por...@caplab.org doug.por...@caplab.org

  http://www.caplab.org/ www.caplab.org



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RE: [Histonet] Gayle Callis, You Made Our Monday Morning!!

[Shirley A. Powell]

Oh where is that Like button?
sp

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of gayle callis
Sent: Monday, August 04, 2014 10:36 AM
To: 'Jones, Laura'; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Gayle Callis, You Made Our Monday Morning!!

You have me laughing at myself!!!  I'll  blame the computer for the typo
error and  spend the rest of my day laughing at myself.This isn't the
first time this has happened in one of my posts.  Whatever you do, do NOT make 
the mistake of misspelling fuchsin or your good friends will come
back and haunt you with that f-bomb  mistake.   I laughed even harder then
but am more careful when typing out that word. 

 Busy?   I don't think I was referring to any feminine anatomical features
we all know so well.   I am quite sure some histo guys are laughing too.   

Still chuckling and glad I provided some unintentional  amusement in that
lengthy post.   
 
Gayle Callis ;)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jones, Laura
Sent: Monday, August 04, 2014 7:51 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Gayle Callis, You Made Our Monday Morning!!

From Gayle's very knowledgable post about storage of Schiffs:

However, if I had to have immediate PAS staining in a busty lab, 

We would like a definition of busty, please!!  Thank you for the laugh!


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RE: [Histonet] Peggy Wenk

[Shirley A. Powell]

Thanks Dave.  Yes she was so brave.  Also HistoTalk is a great venue for 
histotechs all over the world to get to hear great educators and histology 
professional champions interviewed by you.  Younger techs will see their names 
many times but may never be able to relate to or get to know their good 
examples set for our profession.  But thanks to Histotalk they can go back and 
listen to what these former ones have accomplished and how they did it.  Such 
an inspiration.  

Shirley

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of David Kemler
Sent: Monday, July 28, 2014 9:35 PM
To: Histonet
Subject: [Histonet] Peggy Wenk

Peggy's last interview on HistoTalk www.histotalk.com was taped last year at 
the NSH. During her interview, I stopped taping several times, because the 
tears and my choking up were too much for me. Each time I stopped, Peggy smiled 
at me and said 'It's Ok, Dave, it's OK.

It's a shame HistoTalk never got the recognition it truly deserved. Interviews 
like Peggy's have meaning for everyone in the profession. Over the years, 
HistoTalk has had a small group of champions - Peggy was one of them! God 
bless. To Mr. Peggy Wenk, May the Universal Power be within you and your 
loved ones, now and always.

Dave
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RE: [Histonet] Peggy Wenk's passing

[Shirley A. Powell]

I agree, she was such a great example of professionalism for the histology 
community.  She will be greatly missed.  

Shirley Powell

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A
Sent: Monday, July 28, 2014 10:34 AM
To: Lee  Peggy Wenk; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Peggy Wenk's passing

I am sorry to hear of our loss in the Histology world and more importantly of a 
truly good person in our lives. 

Pam Marcum

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee  Peggy Wenk
Sent: Monday, July 28, 2014 8:48 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Peggy Wenk's passing

It is my sad duty to tell you that Peggy Wenk passed away peacefully Saturday 
morning.  Her husband and companion of 37 years and her sister (from Bend,OR) 
were there.

As you know Peggy had a large influence in the world of Histology.

She also put herself into her church serving over the years as a nursery school 
director, Lay Eucharistic Minister and on the vestry. 
She sang enthusiastically in the choir and played in the newly formed bell 
choir.

Because of our love of books, we both volunteered at the local library.
We helped collect books, sort them and then helped to sell them; raising funds 
for the library's use.

There will be two memorial services: one here in Michigan for all her local 
family and coworkers, the second in southern California (a burial at sea).  We 
are asking that no flowers be sent; instead Peggy has specified (she and her 
sister planned the funeral several months ago) three different charitable 
organizations in lieu of flowers.

St. Mary's-in-the-Hills Episcopal Church (Peggy's church)

Shades of Pink Foundation (financial aid to local breast cancer patients)

Peggy A. Wenk Endowed Scholarship for Histotechnology at Oakland University

Please respond to this email if you'd like more information on the services or 
on giving (or to l...@lpwenk.net).

Thanks
Lee Wenk (Mr. Peggy Wenk;)
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[Histonet] RE: Re: Friday histology trivia

[Shirley A. Powell]

Most of you guys are too young to remember Quincy, who told his lab assistant 
that if he did not come up with an answer he would be demoted to the histology 
lab to count specimens.  Never watched that show again.  Good thing the writers 
were on the other side of the country at that time.  But hey I have mellowed 
since then.  All will agree that medical shows take license with truth and 
reality in view of the almighty $$$.  

Shirley   

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, 
Thomas
Sent: Friday, June 27, 2014 1:54 PM
To: Sanders, Jeanine (CDC/OID/NCEZID); 'Morken, Timothy'; 'Teri Johnson'; 
'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Re: Friday histology trivia

The last medical show I watched was ER. Sent NBC an irritated e-mail after the 
episode where Dr. Wylie gave a resident a tube of blood and told her to take to 
the lab and wait there for the results since the lab loses everything. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, 
Jeanine (CDC/OID/NCEZID)
Sent: Friday, June 27, 2014 1:30 PM
To: 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Re: Friday histology trivia

Remember the episode of House where the physicians assisting House dropped some 
red liquid on a slide and had an immuno?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Friday, June 27, 2014 1:25 PM
To: 'Teri Johnson'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Re: Friday histology trivia

Are all news stories are as faked as the ones showing something in a lab? One 
had two doctors(?) in lab coats peering at a microscope slide they are holding 
to the light above their head and the reporter is saying they are examining 
samples from a cancer patient. 

Wow, good eyesight!

Tim Morken
Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San 
Francisco Medical Center San Francisco, CA

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri Johnson
Sent: Friday, June 27, 2014 10:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Friday histology trivia

Carefully placed scientific equipment, as well as spokespeople dressed in lab 
coats, are great marketing tools for anything touted to cure us or make us 
healthier.

Next time you watch an actual medical or research-based news story, check out 
how they ALWAYS show someone pipetting. Always.
It's become a game for me to spot it.

Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752

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RE: [Histonet] Histology as art!

[Shirley A. Powell]

That would be Mequita Praet, does a beautiful job. 
Shirley

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice 
Frederick
Sent: Friday, June 27, 2014 2:29 PM
To: Emily Brown; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Histology as art!

Love it! There is a tech that makes jewelry that sells at NSH. Made in a 
variety of special stains...

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Brown
Sent: Friday, June 27, 2014 10:58 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Histology as art!

Hello Histonetters,

I'm really looking forward to going to the brand new Morbid Anatomy Museum in 
NYC, but imagine my surprise when I found some histology in their online gift 
shop!!
http://morbidanatomy.bigcartel.com/category/gifts
Histology is beautiful, but it is odd to look at those images on clothing.

Emily



By bitching and bitching and bitching, they could exhaust the drama of their 
own horror stories. Grow bored. Only then could they accept a new story for 
their lives. Move forward.

-Chuck Palahniuk, Haunted
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RE: [Histonet] RE: Re: Friday histology trivia

[Shirley A. Powell]

Yep you may be old as me.  :)


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Ann 
Jones
Sent: Friday, June 27, 2014 2:39 PM
To: Mike Andrews; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Re: Friday histology trivia

Ha! I can beat that~ we have a cryostat that is at least 50+ years old, old AO 
microtome cryostat. Still works, barely. . .looks just like the old fashion 
ice-cream holders. (P.S. Prob not true to life, but I liked Quincy-did I just 
date myself?)

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 6/27/14, 12:35 PM, Mike Andrews udsd...@gmail.com wrote:

One of my current is the (Salonpas?) ad I which there is a very obvious 
AO Series 10 just behind the presenter. Good old brass'n'glass, but 
hardly current -- even if I do own a few.

Mike Andrews, W5EGO
WWME Oklahoma area executive team

 On Jun 27, 2014, at 12:25 PM, Morken, Timothy
timothy.mor...@ucsfmedctr.org wrote:
 
 Are all news stories are as faked as the ones showing something in a 
lab? One had two doctors(?) in lab coats peering at a microscope slide 
they are holding to the light above their head and the reporter is 
saying they are examining samples from a cancer patient.
 
 Wow, good eyesight!
 
 Tim Morken
 Supervisor, Electron Microscopy and Neuromuscular Special Studies UC 
 San Francisco Medical Center San Francisco, CA
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri 
Johnson
 Sent: Friday, June 27, 2014 10:15 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Re: Friday histology trivia
 
 Carefully placed scientific equipment, as well as spokespeople 
dressed in lab coats, are great marketing tools for anything touted to 
cure us or make us healthier.
 
 Next time you watch an actual medical or research-based news story, 
check out how they ALWAYS show someone pipetting. Always.
 It's become a game for me to spot it.
 
 Teri Johnson
 Manager, Histology
 Genomics Institute for
 Novartis Research
 Foundation
 San Diego, CA
 858-332-4752
 
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RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours

[Shirley A. Powell]

Oh where is the like button.  :)


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Friday, June 06, 2014 12:22 PM
To: Conway, Carla; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours

Not since 1978. (Sorry - couldn't resist - Happy Friday!)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla
Sent: Friday, June 06, 2014 9:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours

Hello everyone,

When it is working correctly, our tissue processor moves a basket of tissues 
through 10 reagent containers. Last night it malfunctioned and suspended the 
tissues above the 80% ethanol container. The tissues were high and dry for ~ 9 
hours (!) until I placed them into 70% ethanol this morning. I will process 
them next week. Has this happened to anyone else and what tissue artifacts can 
I expect?

Thanks very much,

Carla





Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-2042
Fax: 206-526-6654
E-mail: cmcon...@usgs.gov
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RE: [Histonet] Basement Lab

[Shirley A. Powell]

Because we are explosive and flammable?  I know it is terrible, 
they need us but want to hide us.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
susan.wal...@hcahealthcare.com
Sent: Monday, May 05, 2014 3:19 AM
To: suetp...@comcast.net; jrsmallw...@bell.net
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Basement Lab

Why do they always want to put us in the basement? We have a lot of the 
hospitals explosives and flammables??
Still, as long as they have a good ventilation (you often need a roof or 
outside wall to do this) it might be ok. I hope they have good environmental 
engineers.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Saturday, May 03, 2014 3:51 PM
To: John Smallwood
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Basement Lab

Not a good idea

Sent from my iPhone

 On May 3, 2014, at 12:10 PM, John Smallwood jrsmallw...@bell.net wrote:
 
   Our small Hospital with growth plans, is considering a new Laboratory in 
 the basement of the planned tower. I consider this a less than desirable 
 location. Spills , fumes, chemical allotments etc. What are Histonet members 
 thoughts and ideas ??
 
 Than you,
 John Smallwood, MLT.
 London, Ont. Can.
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[Histonet] Is there a way to post pictures of slides on histonet? Or to make them available for people to look at?

[Shirley A. Powell]

HI Patrick, Marvin Hanna posted these instructions in the below message on 
histonet yesterday.  This is a good way to post images you wish to discuss on 
histonet.  He has made available a way to do this for the histonet community, 
as well as has the archives of histonet on the www.histosearch.com website.  
You cannot post images on histonet. 

Shirley Powell


 Hi Histonetters,

 Tim Morken asked me about posting images for discussion on Histonet a couple 
 of weeks ago and we noticed our images website is no longer running. As we 
 move into digital pathology, having the ability to post images to discuss on 
 Histonet is important. So, we set up an easy way to post images for Histonet 
 on Histosearch at http://histosearch.com/imageupload/. You need to fill in 
 your name, a title for your images, select images to upload from your 
 computer or smartphone and click submit. There is no need to register and it 
 takes about 2 minutes. We intentionally did not include comments on the 
 website so the discussions will take place on Histonet.

 We have also set up mobile pages for searching Histosearch and the archives 
 at http://m.histosearch.com and http://m.histosearch.com/histonet/ for 
 histologists on the go. You may also want to try out the new image searching 
 capabilities on Histosearch. After searching with keyword(s) such as ki67 or 
 alcian blue, there is a link at the top of the results page for images.
 Clicking this will provide images related to your keyword(s) from the over 12 
 million histopathology related pages searched in Histosearch. We believe we 
 have the largest list of histology educational resources on the internet at 
 Histosearch and they are searchable for text and images.
 Let us know if we've missed any histology resources.

 Best Regards,

 Marvin Hanna


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick
Sent: Wednesday, April 30, 2014 1:43 PM
To: 'Histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Is there a way to post pictures of slides on histonet? Or 
to make them available for people to look at?

Hi everyone,

What is a good way to post pictures of slides for you guys to look at?

I had a weird black precipitate in one of my IHCs and I'd like for you guys to 
look at them and see if you recognize what this artifact is?

Thanks

Patrick.

PS: I tried uploading them as attachments but the size was too large even 
though I tried to crop things to keep the size to a minimum.

Thanks

Patrick.


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[Histonet] RE: job postings

[Shirley A. Powell]

www.histosearch.com has a histology job page so that you can list or acquire 
jobs.  
Shirley Powell

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Loss
Sent: Tuesday, December 17, 2013 2:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] job postings

Hello all,

Where does everyone find the most success when advertising job openings for 
your histology labs?  We currently use Careerbuilder, Monster and also do some 
advertising with NSH and a few other places.  We want to make sure we're 
hitting the places where the most people are looking.  Any thoughts are 
appreciated.

Thanks,

Lee Loss
Lab Manager
Dermatology Associates | Forefront Dermatology 
ll...@dermwisconsin.commailto:ll...@dermwisconsin.com




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RE: [Histonet] RE: Yahoo link

[Shirley A. Powell]

I had the same experience, the clinical lab techs asked the supervisor to keep 
our door closed so they would not have to smell the fumes, just lock us up in 
it. I was considering calling EPA in to check it out.   Thank goodness those 
days are gone.  Use those fume hoods and all the other ppe you can get.  :)
Shirley Powell

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Wednesday, December 04, 2013 10:57 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Yahoo link

I worked in a non-ventilated lab once - back in the 70s - just before the 
xylene study was released. I knew something was wrong and I knew it was xylene 
and finally was able to get an exhaust put in the window . I could taste it, 
smell it, and seemed to cough it up!! And my brain was fuzzier than my usual 
fuzz! The lab director's office was next to my lab and she moved, because she 
said she couldn't stand to be next to me!

So thankful for good regs now and labs since then that have been very well 
ventilated. We have come a long way, baby!!

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Wednesday, December 04, 2013 9:52 AM
To: 'Lee  Peggy Wenk'; Elizabeth Cameron; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Yahoo link

I remember participating in the health study in the 80's.   Xylene and 
Formaldehyde levels are monitored in all labs.  If our hospitals/research 
centers would allow us to forward that information on to someone who could 
compile data it would be a starting place for a health study.

I strongly believe this profession can be danger to health.  I can name at 
least 10 histotechs who have died from cancer.  Yes, it may have been when they 
were older but I feel certain it was from all the chemical exposure in 
histology before safety became a priority.   I know others will remember no 
ventilation, smoking and eating in the lab, no real requirements for gloves, 
etc. There were also no MSDS information available and we were exposed 
through lack of knowledge.

I believe the lab is much safer today with all of the safety precautions we 
take and the knowledge we have on chemicals/stains and their toxicity.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas 
Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee  Peggy Wenk
Sent: Wednesday, December 04, 2013 8:24 AM
To: Elizabeth Cameron; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Yahoo link

Couple of studies that I know of.

One was sponsored by NSH in the mid-1980's. KH Kilburn came to several NSH 
Symposiums, and did different tests on people who volunteered to participate. 
Published findings in the late 1980's that said that histotechs had lower 
pulmonary function than average population, and decreased memory, equilibrium 
and dexterity than the general population.

In Letters to the Editor, people pointed out statistical flaws (low numbers of 
participants, for example). I also feel there were flaws, such as testing 
people after traveling over time zones, who were up late at the parties, and 
had possibly been drinking the night before. There was no way to measure how 
much exposure to formaldehyde or xylene people were really exposed to. I didn't 
participate, but if I though the amount I was being exposed to was medium, 
someone else being exposed to the same amount might have said low amount and 
someone else could have said high amount. And the studies would say therefore 
the low pulmonary exposure was due to histotechs being exposed to formaldehyde. 
But who could say it was due to that chemical, and not due another chemical, or 
due to the fact that at the same time, people were smoking in the lab I was 
working in, which was a small space.

Another study somewhat relates - S Khattak in 1999 wrote one on pregnancy 
outcomes following gestational exposure to organic solvents. They interview 
women who were

[Histonet] Yahoo link

[Shirley A. Powell]

When I entered the profession I was told the average life expectancy of a 
histotechs was 20 years from hiring.  That scared me but I was already hooked.  
I have been doing this 51 years, so maybe good laboratory practices can help, 
in spite of bad ventilation and all those other dangers mentioned. 

Shirley Powell
Antique Histotech

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Tuesday, December 03, 2013 11:40 AM
To: Morken, Timothy; Histonet
Subject: Re: [Histonet] RE: Yahoo link

Just what I was thinking. We will never get enough new students now!

With proper training and equipment, we do the job safely!




And you can drown in an inch of water. Is that in the MSDS!
 
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
5830 N Blue Lake Dr. Please note new address!
Norman, OK 73069
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com



 From: Morken, Timothy timothy.mor...@ucsfmedctr.org
To: 'Mike Tighe' mti...@trudeauinstitute.org; 
histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) 
histonet@lists.utsouthwestern.edu 
Sent: Tuesday, December 3, 2013 10:24 AM
Subject: [Histonet] RE: Yahoo link
 

Great. Just what we need. 


Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 
3H 2 O since we know that suitable precautions preclude most of the danger. For 
instance, I'll have to say that the histo lab here is wonderful in that it has 
such good ventilation (ie.,  extraction) that there is none of the usual 
chemical smell - no xylene, alcohol, specials chemicals that often assaults the 
senses in histology. Vendors that come here are amazed.

Tim Morken
Supervisor, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Tighe
Sent: Tuesday, December 03, 2013 6:31 AM
To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Yahoo link

Anybody wonder who has the most harzardous job to your health? We're Number 
One!!!



http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html



Mike
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[Histonet] RE: Yahoo link

[Shirley A. Powell]

Thanks Tim.  You are sweet.  
People keep asking me when I was going to retire and I told them never, they 
will have to carry me out feet first.  I think loving my job has kept me going 
this long.  :)
sp


-Original Message-
From: Morken, Timothy [mailto:timothy.mor...@ucsfmedctr.org] 
Sent: Tuesday, December 03, 2013 12:22 PM
To: Shirley A. Powell; Histonet
Subject: RE: Yahoo link

Well, Shirley, you are actually an Angel, so nothing will ever stop you!! (from 
an old Georgia Society hand).

Tim Morken
Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San 
Francisco Medical Center San Francisco, CA


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. 
Powell
Sent: Tuesday, December 03, 2013 9:18 AM
To: Histonet
Subject: [Histonet] Yahoo link

When I entered the profession I was told the average life expectancy of a 
histotechs was 20 years from hiring.  That scared me but I was already hooked.  
I have been doing this 51 years, so maybe good laboratory practices can help, 
in spite of bad ventilation and all those other dangers mentioned. 

Shirley Powell
Antique Histotech

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Tuesday, December 03, 2013 11:40 AM
To: Morken, Timothy; Histonet
Subject: Re: [Histonet] RE: Yahoo link

Just what I was thinking. We will never get enough new students now!

With proper training and equipment, we do the job safely!




And you can drown in an inch of water. Is that in the MSDS!
 
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
5830 N Blue Lake Dr. Please note new address!
Norman, OK 73069
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com



 From: Morken, Timothy timothy.mor...@ucsfmedctr.org
To: 'Mike Tighe' mti...@trudeauinstitute.org; 
histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) 
histonet@lists.utsouthwestern.edu
Sent: Tuesday, December 3, 2013 10:24 AM
Subject: [Histonet] RE: Yahoo link
 

Great. Just what we need. 


Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 
3H 2 O since we know that suitable precautions preclude most of the danger. For 
instance, I'll have to say that the histo lab here is wonderful in that it has 
such good ventilation (ie.,  extraction) that there is none of the usual 
chemical smell - no xylene, alcohol, specials chemicals that often assaults the 
senses in histology. Vendors that come here are amazed.

Tim Morken
Supervisor, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Tighe
Sent: Tuesday, December 03, 2013 6:31 AM
To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Yahoo link

Anybody wonder who has the most harzardous job to your health? We're Number 
One!!!



http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html



Mike
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[Histonet] Ciliated Protozoa

[Shirley A. Powell]

I am way out of my league here and need help from the histonet world.  I have 
been asked to do wet and dry silver nitrate-protargol staining for a research 
project on ciliated protozoa.  I need to speak with someone out there who has 
done this before.  You may contact me directly.

Thanks

Shirley A. Powell, HT(ASCP)HTL, QIHC
Technical Director
Histology Curricular Support Laboratory
Mercer University School of Medicine
1550 College Street
Macon, GA  31207
478-301-2374 Lab
478-301-5489 Fax

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RE: [Histonet] Bone Marrow Issues

[Shirley A. Powell]

I had this happen when I was doing IHC and changed to regular slides and used 
StayOn from Leica. Had more success with keeping the tissue on the slides.  I 
now do autopsy slides and do the same.

Shirley

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jordan Phillips
Sent: Tuesday, May 28, 2013 7:56 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bone Marrow Issues

HI everyone.  We are currently having problems with all of our bone marrows, 
mainly clots, washing off the slides.  We fix our bone marrows in Zenkers.  We 
use positive charged slides, put the slides in the oven for a minimum of 2 
hours and hand depar with no agitation.  When we get to the water, the sections 
just float off the slides, leaving just a rim of the tissue.   Any tips or 
suggestions would be greatly appreciated! 
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[Histonet] IHC question

[Shirley A. Powell]

I need to talk to any histotechs/researchers out there who are doing IHC on 
shellfish or fish.   Please contact me personally at 
powell...@mercer.edumailto:powell...@mercer.edu.  Also any vendors who sell 
detection systems for them, please contact me.


Shirley A. Powell, HT(ASCP)HTL, QIHC
Technical Director
Histology Curricular Support Laboratory
Mercer University School of Medicine
1550 College Street
Macon, GA  31207
478-301-2374 Lab
478-301-5489 Fax

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RE: [Histonet] GSH Symposium

[Shirley A. Powell]

GSH would like to thank you for coming.  Glad you had a good time and we will 
let you know when the next meeting is and the dates as soon as we get them.  
Crash our party anytime.  

Shirley Powell

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Deloris Carter
Sent: Tuesday, April 16, 2013 12:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] GSH Symposium

I'd like to say thank you to everyone at the GSH Symposium for making this 
Yankee feel welcome. I had a great time and found the sessions very helpful. 
Thanks again for letting me crash the party!
Deloris Carter
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[Histonet] Updates on Jekyll Island

[Shirley A. Powell]

Waaay too much information Jack, just keep it covered.  You can 
bring all those wonderful photos of your European trip with your Dad.  I do 
hope you have made your reservations at Jekyll.  Sounds like the rooms are 
going fast.  

See you at the beach.  Oh and Jack, they will arrest you if you show too much.  
This is Georgia you know.  

Sp

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff
Sent: Thursday, March 28, 2013 3:17 PM
To: Zimmerman, Billie; Histonet
Subject: RE: [Histonet] Updates on Jekyll Island...Procrastinators please read 
this ASAP

PLEASE! PLEASE! PLEASE! I want that room! 
Oh and since you mentioned speedos and crochet bikinis, you want me to bring my 
white mankini with the British flag on the front? Just kidding, I think I had 
better leave that at home! 
Jack

Jack L. RatliffOwner/Histologist, Ratliff Histology Consultants, LLCChairman, 
Hard Tissue Committee - National Society for Histotechnology
389 Nichol Mill LaneFranklin, TN 37067(317) 281-1975 (c)(615) 236-4901 (o)(615) 
236-4962 (f)jratl...@ratliffhistology.com


 From: bzimm...@gru.edu
 To: histonet@lists.utsouthwestern.edu
 Date: Thu, 28 Mar 2013 18:57:46 +
 Subject: [Histonet] Updates on Jekyll Island...Procrastinators please read
 this ASAP
 
 If you haven't reserved your room for the upcoming GSH meeting next month, 
 please note that all island and standard Oceanside rooms have been reserved.  
 But,  just because you snoozed, were waiting on money for your institution, 
 or you're just a classic procrastinator, there are still options.  There are 
 upgraded rooms such as the lanai.  This room has a Jacuzzi, microwave, King 
 size bed, small refrigerator, and a private balcony or patio. There's also 
 the efficiency which has the kitchenette with a stove top, refrigerator,  two 
 double beds, sitting area, and private balcony or patio.  The symposium rate 
 still applies and if you have issues or concerns, contact Linda Schepps at 
 Oceanside.  She can't cure procrastination but she can help secure a room for 
 you.
 
 While you're strolling around the historic area of the island in your speedo 
 or crochet bikini, check out Becky's famous chicken salad.  It's a little 
 place with outside tables.  Just walk up to the window and place your order.  
 I attempted to have a chicken salad sandwich there but they were all sold 
 out!! The lady managed to let me sample a teaspoon of it. It was delicious 
 and didn't taste like that stuff in the grocery store.  I plan to attempt 
 another order when I return next month!!
 
 
 Augusta State University and Georgia Health Sciences University have 
 consolidated to become Georgia Regents University. Effective January 9, 2013, 
 my email address has changed to bzimm...@gru.edu. Please update your address 
 book to reflect this change.
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[Histonet] Question on Estrogen antibodies for fish

[Shirley A. Powell]

I am asking this for a researcher.  Please respond to her at 
klar_elizab...@columbusstate.edumailto:klar_elizab...@columbusstate.edu. Her 
name is Ely Klar.

She is looking for any form of estrogen antibody that works on bass fish 
gonads.  Vendor's responses welcome.


Shirley A. Powell, HT(ASCP)HTL, QIHC
Technical Director
Histology Curricular Support Laboratory
Mercer University School of Medicine
1550 College Street
Macon, GA  31207
478-301-2374 Lab
478-301-5489 Fax

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RE: [Histonet] Re: best controls

[Shirley A. Powell]

I want to know what happened to all the equipment there, especially the large 
Sartorius Microtome that was in the bone lab.  If anyone has that information 
please email me.  

Shirley

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren
Sent: Monday, June 11, 2012 1:34 AM
To: Bob Richmond
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: best controls

I wonder what happened to all the leprous armadillos living at AFIP?

  Heeere little feller

   Jay A. Lundgren, M.S., HTL (ASCP) 
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[Histonet] old equipment for parts

[Shirley A. Powell]

I have an old Tissue Tek III cryo console that only gets luke cool and a 
ThermoFisher slide dryer that died on me.  If anyone wants these for parts or 
boat anchors you can have them, you pay shipping.  I hate to trash them if the 
parts can be recycled.

sp

Shirley A. Powell, HT(ASCP)HTL, QIHC
Technical Director
Histology Curricular Support Laboratory
Mercer University School of Medicine
1550 College Street
Macon, GA  31207
478-301-2374 Lab
478-301-5489 Fax

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[Histonet] Histo position available in Georgia

[Shirley A. Powell]

Posting for a friend.  Please respond to the below contact information.

Histotechnician/technologist position available at HCA Coliseum Medical 
Centers, Macon, GA. Full-time. Monday-Friday. At least one year experience 
preferred. Apply on line at 
www.coliseumhealthsytem.comhttp://www.coliseumhealthsytem.com  or call 
Redonna Bunch at 478-464-5486.



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[Histonet] Region III final reminder

[Shirley A. Powell]

Hi Histotechs,

This email is to remind you of the Georgia Society for Histotechnology hosting 
Region III meeting at Callaway Gardens, Pine Mountain GA April 13-15th,.  This 
is next week friends.  We will NOT be in the conference center as last year, 
and that is a GOOD thing.  We will be in the ballroom area where we will have 
more space.  We have far exceeded our original block of rooms, extended the 
deadline and now if the Mountain Creek Inn has any available, they will allow 
the GSH room rate.  Please use the GSH Group # # 78K711 to get the discounted 
room rate of $109.  Make reservations now at 1-800-225-5292.

If you have not registered for the meeting please do so.PLEASE REGISTER NOW 
AS AWAITING FUNDS  if you are waiting approval for the meeting go ahead and 
register for your workshops, luncheon, dinner and note on the form that you are 
awaiting funds  below the total line.  When approved, then send the funds, or 
if time does not allow, pay on site.  But please register as soon as possible, 
don't wait, workshops are filling up fast.

  We also will have Histotalk's Dave Kemler at the meeting to interview 
some of our attendees as well.

  Also make plans to attend the Carriage and Horses Dinner on Saturday 
night for great food, great friends and good entertainment.  The deadline for 
reservations for the dinner is April 9th, that is next Monday.  Please make 
sure you fill out your registration form completely and email the form to Anne 
Taylor, GSH Treasurer to confirm your workshops as awaiting funds and then pay 
at the registration desk on site since time is short.

  If you have questions or concerns please contact Mike Ayers at 
lmay...@charter.netmailto:lmay...@charter.net,  Wanda Simons at 
wandr...@att.netmailto:wandr...@att.net or myself at 
powell...@mercer.edumailto:powell...@mercer.edu.

 We are excited to have 29 vendors exhibiting at our meeting and more 
signing up as we approach the meeting.

BioCare  ~Sponsor of  new  IHC Award

BioGenex

B/R Instruments

Cancer Diagnostics

Cell Marque

Choice Medical

Clarient~ sponsoring dinner at Dagher's

Dako

EMS

Epitomics

General Data

IMEB

Lab Storage

Leica Microsystems  ~ sponsoring dinner at Dagher's

Leica Biosystems

Mopec

PolyScientific RD - sponsor of new award TBA

Sakura - Sponsor of GSH Histotechnologist of the Year Award

Stat Lab

Southeast Pathology

Thermo Fisher -

TBS

ScyTek

StatLab

Ventana



Those Not attending but supporting GSH are:

Anatech - Unmanned table

CL Sturkey - Door prizes

LABSCO - Sponsoring a break

Newcomer - Sponsoring a Break and provided totes

Come Experience 
 Histotechnology - Southern Style



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[Histonet] Say Just for a while

[Shirley A. Powell]

Well I bet you will be back in a week.  This histology family is hard to stay 
away from.  :)  
I am coming up on 50 years in July, but not ready to leave it.  
Shirley

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Friday, March 30, 2012 9:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Farewell

Hard as it is to believe - and I probably won't until I've been away for a week 
or more - this is my LAST day to work.  Retirement? Who knew it would come 
around so fast? I remember having only fifteen years left to work and that was 
just yesterday!  Histology has been 'bery, 'bery good to me and although I fell 
into it by accident, it has been a fascinating, involving, liberating 
experience.  I'll be lurking on Histonet but under the alias of nmhisto and 
I'll probably do some p.r.n.
work but I'm going home and throwing away my alarm clock and resetting the 
coffee maker for 6:00 a.m. instead of 3:45 a.m.  Thank you all for your advice, 
guidance, humor, relative insanity and wisdom.  Enjoy what you do and try to 
recruit at least one person into this field before it's your time to pick out 
the color of the tennis balls on your walker.

 

Hail and Farewell and best of everything to every one of you.

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

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[Histonet] GSH-Region III meeting Callaway Gardens GA

[Shirley A. Powell]

Hi everyone,


I want to remind you of the Georgia Society for Histotechnology hosting Region 
III meeting at Callaway Gardens, Pine Mountain GA April 13-15th,.



Three important points:



1. PLEASE RESERVE YOUR ROOM BEFORE MARCH 13TH, AS WE HAVE ADDED ANOTHER 
BLOCK OF ROOMS~ We exceeded the number of rooms blocked and have added another 
block.  The Deadline for releasing these rooms and for receiving the discounted 
hotel rate is March 13th, so act now.  Remember The Mountain Creek Inn fills up 
fast during the spring which is golfing season here in Georgia.  Make 
reservations now at 1-800-225-5292 and use the GSH group # 78K711 to get the 
discounted room rate of $109.  There are other rooming option rates in the 
program which can be found at http://www.histosearch.com/gsh/symposium.html.


2.PLEASE REGISTER NOW AS AWAITING FUNDS  if you are waiting approval for 
the meeting go ahead and register for your workshops, luncheon, dinner and note 
on the form that you are awaiting funds  below the total line.  When approved, 
then send the funds, or if time does not allow, pay on site.  But please 
register as soon as possible, don’t wait.



3.  We are excited to have 23 vendors exhibiting at our meeting.

They are:



BioCare  ~Sponsor of  new  IHC Award

BioGenex

B/R Instruments

Cancer Diagnostics

Cell Marque

Clarient~ sponsoring dinner at Dagher's

Dako

EMS

Epitomics

General Data

IMEB

Lab Storage

Leica Microsystems  ~ sponsoring dinner at Dagher’s

Leica Biosystems

Mopec

PolyScientific RD

Sakura – Sponsor of GSH Histotechnologist of the Year Award

hermoFisher

TBS

StatLab

Ventana



Not attending but supporting GSH are:

Newcomer – Sponsoring a Break and provided totes

CL Sturkey – Door prizes

Come experience….

Histotechnology – Southern Style

Shirley Powell
GSH Secretary


Shirley A. Powell, HT(ASCP)HTL, QIHC
Technical Director
Histology Curricular Support Laboratory
Mercer University School of Medicine
1550 College Street
Macon, GA  31207
478-301-2374 Lab
478-301-5489 Fax

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[Histonet] GSH meeting weekly reminder

[Shirley A. Powell]

Hi Histonetters and fellow histotechs,

I am making sure you don't forget about our meeting coming up.  The popular 
workshops are filling up fast.  The workshop fee is all inclusive, $100 for a 
possible six is a great deal.  Most individual workshop fees are around $40 
each.  Don't miss out, earn your CEUs at the Georgia Society for 
Histotechnology -  Region III meeting at Callaway Gardens, Pine Mountain GA 
April 13-15th,.

The Deadline for receiving the discounted hotel rate is March 13th, so act now. 
 Remember The Mountain Creek Inn fills up fast during the spring which is 
golfing season here in Georgia.  Make reservations now at 1-800-225-5292 and 
use the GSH group # 78K711 to get the discounted room rate of $109.  There are 
other rooming option rates in the program which can be found at 
http://www.histosearch.com/gsh/symposium.html.

Plan to attend, bring the family for a vacation, Callaway Gardens is a 
wonderful family experience.

If you have any questions please contact GSH President - Mike Ayers at 
lmay...@charter.netmailto:lmay...@charter.net, GSH  Vice President and 
Exhibit Liaison - Wanda Simons at wandr...@att.netmailto:wandr...@att.net or 
myself powell...@mercer.edumailto:powell...@mercer.edu.

Come to Georgia and experience

Histotechnology - Southern Style

Shirley Powell
GSH Secretary


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[Histonet] new billing Codes

[Shirley A. Powell]

Hi Histonetters, I have a great idea.  You can come to Georgia for our meeting, 
our own Joyce Weems will be presenting a workshop on CPT coding at our meeting. 
 

The Georgia Society for Histotechnology -  Region III meeting will be held at 
Callaway Gardens, Pine Mountain GA April 13-15th,.  The Deadline for receiving 
the discounted hotel rate is March 13th, so act now.  Remember The Mountain 
Creek Inn fills up fast during the spring which is golfing season here in 
Georgia.  Making reservations with a credit card will hold your room, they will 
not apply the rate until you actually check in.  Call now 1-800-225-5292 and 
use the GSH group # 78K711 to get the discounted room rate of $109.  There are 
other rooming option rates in the program which can be found at 
http://www.histosearch.com/gsh/symposium.html.  

Also register early for the fantastic workshops GSH has lined up, like Joyce's 
and earn your CEUs.  GSH has planned a great awards luncheon on Saturday as 
well as a dinner on Saturday night, details are in the program.  Payment is 
easy with PayPal.  You do not have to have an account with them to use this 
service and it is secure.  Make payment by going to www.PayPal.com and send 
payment to the email address on the registration form.  

Plan to attend, bring the family for a vacation, Callaway Gardens is a 
wonderful family experience.

If you have any questions please contact GSH President - Mike Ayers at 
lmay...@charter.net, GSH  Vice President and Exhibit Liaison - Wanda Simons at 
wandr...@att.net or myself powell...@mercer.edu.

Please pass this invitation to any who may not get this email.  

Come to Georgia and experience

Histotechnology - Southern Style

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Tuesday, February 14, 2012 10:55 AM
To: Goins, Tresa; anita dudley; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] new billing

Same antibody on multiple blocks of same specimen was what I was referring to. 
For the past couple of years, we could charge per block. Now it is per specimen.

However, it is still per block for special stains. Just to be sure that is 
clear...


Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax




From: Goins, Tresa [mailto:tgo...@mt.gov]
Sent: Tuesday, February 14, 2012 10:48
To: Weems, Joyce; anita dudley; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] new billing


Now I am confused.  Did you mean to state same antibody on multiple slides of 
the same specimen rather than multiple blocks of the same specimen?



I am asking because of questions from breast cancer patients who have 
difficulty getting coverage from their insurance companies when multiple lymph 
nodes are submitted from a single surgery.



Thanks,



Tresa



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Tuesday, February 14, 2012 8:36 AM
To: anita dudley; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] new billing



You can charge for each antibody on each specimen. You cannot charge for the 
same antibody on multiple blocks of the same specimen.



e.g. Sentinel node - Melan A, S100, HMB45

A1 - A4 = 3 charges not 12.



Hope this makes sense..



Joyce Weems

Pathology Manager

Saint Joseph's Hospital

5665 Peachtree Dunwoody Rd NE

Atlanta, GA 30342

678-843-7376 - Phone

678-843-7831 - Fax





-Original Message-

From: 
histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley

Sent: Tuesday, February 14, 2012 10:30

To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu

Subject: [Histonet] new billing





can someone explain the new billing for the antibodies on blocks?  not sure 
what they mean.  can you just bill one antibody per block even tho

you may do three or four?   thanks so much

anita dudley

providence hosp

mobile alabama

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[Histonet] Job in AZ

[Shirley A. Powell]

Asked to pass this on.
Opportunity in AZ

Full time Histotech opportunity w/Cancer Treatment Centers of America in 
Goodyear, AZ?  This is a great opportunity offering an annual bonus, 
competitive pay and full (attractive) benefits.
Requires Bachelor's Degree in Biological/Physical Sciences or equivalent; 
minimum of 2-5 yrs experience as a Histotechnologist and ASCP registration 
preferred.
A national network of hospitals providing a comprehensive, fully integrative 
approach to cancer care. CTCA serves patients with advanced cancer from all 50 
states at facilities located in suburban Chicago, Philadelphia, Tulsa and 
suburban Phoenix. CTCA offers state-of-the-art oncology treatments combined 
with complementary therapies to treat the disease and help improve each 
person's quality of life.

CTCA is committed to becoming the best place to work. One way that we strive to 
achieve this goal is by offering a full spectrum of benefits which include: 
medical, dental, Healthy Awards Program, vision, flexible spending accounts, 
basic life and ADD Insurance, supplemental life and ADD insurance, short and 
long-term disability insurance, long-term care insurance, supplemental 
voluntary benefits, 401(k) savings and retirement plan, stakeholder assistance 
programs, adoption assistance, nutritional supplements, educational benefits, 
paid time off and an attractive incentive compensation program.

To learn more about this opportunity and to apply online, click on the link:  
http://bit.ly/ypjhC8  (Histotech/Lab)

Any question, you may call me directly at (651) 769-4067. Thank you.

Liz Bornhofen
Sr. Clinical Recruiter
Cancer Treatment Centers of America
(651) 769-4067
lbornho...@cor3talent.commailto:lbornho...@cor3talent.com

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[Histonet] GSH-Region III meeting reminder

[Shirley A. Powell]

Hi Histonetters,

I wanted to remind you guys of the Georgia Society for Histotechnology -  
Region III meeting at Callaway Gardens, Pine Mountain GA April 13-15th,.  The 
Deadline for receiving the discounted hotel rate is March 13th, so act now.  
Remember The Mountain Creek Inn fills up fast during the spring which is 
golfing season here in Georgia.  Making reservations with a credit card will 
hold your room, they will not apply the rate until you actually check in.  Call 
now 1-800-225-5292 and use the GSH group # 78K711 to get the discounted room 
rate of $109.  There are other rooming option rates in the program which can be 
found at http://www.histosearch.com/gsh/symposium.html.

Also register early for the fantastic workshops GSH has lined up; earn your 
CEUs.  GSH has planned a great awards luncheon on Saturday as well as a dinner 
on Saturday night, details are in the program.  Payment is easy with PayPal.  
You do not have to have an account with them to use this service and it is 
secure.  Make payment by going to www.PayPal.comhttp://www.PayPal.com and 
send payment to the email address on the registration form.

Plan to attend, bring the family for a vacation, Callaway Gardens is a 
wonderful family experience.

If you have any questions please contact GSH President - Mike Ayers at 
lmay...@charter.netmailto:lmay...@charter.net, GSH  Vice President and 
Exhibit Liaison - Wanda Simons at wandr...@att.netmailto:wandr...@att.net or 
myself powell...@mercer.edumailto:powell...@mercer.edu.

Please pass this invitation to any who may not get this email.

Come to Georgia and experience

Histotechnology - Southern Style

Shirley Powell
GSH Secretary



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[Histonet] RE: Exam Prep Materials

[Shirley A. Powell]

Found it on Amazon but not familiar with it.

http://www.amazon.com/s/ref=ntt_athr_dp_sr_1?_encoding=UTF8sort=relevanceranksearch-alias=booksie=UTF8field-author=HTL%20Exam%20Secrets%20Test%20Prep%20Team


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lyn Stadler
Sent: Friday, January 27, 2012 3:26 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Exam Prep Materials

Has anyone ever heard of or used the Histotechnology Exam Secrets Study 
Guidehttp://www.mo-media.com/histotech/?  If so, I would appreciate opinions 
on it.

Thanks!



Lyn Stadler
Histology Technician
Department of Histopathology
Cleveland Biolabs, Inc.
73 High Street
Buffalo, NY 14203


 


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[Histonet] You are invited - GSH hosts Region III April 13-15th 2012

[Shirley A. Powell]

Hi Fellow Histotechnology Professionals, Students and Vendors, Please forgive 
me if this is duplicated, it bounced the first time.

I wanted to Invite you to Georgia, the Georgia Society for Histotechnology will 
be hosting the NSH Region III meeting at Callaway Gardens, Pine Mountain GA 
April 13-15th,.  Registration is Friday from 10-12 and the workshops begin at 1 
pm. The last workshops are on Sunday morning.  To view the complete program go 
to http://www.histosearch.com/gsh/symposium.html and print your copy today.  
Make your reservations soon and plan to attend, bring the family for a 
vacation, Callaway Gardens is a wonderful family experience.  Register early 
for the fantastic workshops GSH has lined up; earn your CEUs.  Payment is easy 
with PayPal.  You do not have to have an account with them to use this service 
and it is secure.

Remember The Mountain Creek Inn fills up fast during the spring which is 
golfing season here.  Making reservations with a credit card will hold your 
room, they will not apply the rate until you actually check in.  Call now 
1-800-225-5292 and use the GSH group # 78K711 to get the discounted room rate 
of $109.  There are other rooming option rates in the program also.

***VENDORS:  Vendors can print their registration form from the website to mail 
in and can make payment by going to www.PayPal.comhttp://www.PayPal.com and 
send payment to the email address on the registration form.

There will be a Vendor Reception on Friday night, Awards Luncheon on Saturday, 
and a special dinner event Saturday night.  See the details in the program here 
http://www.histosearch.com/gsh/symposium.html.  Please contact our Vice 
President and Exhibit Liaison, Wanda Simons at 
wandr...@att.netmailto:wandr...@att.net if you have questions or concerns.

If you have any questions please contact Wanda Simons, Mike Ayers or myself.   
Please pass this invitation to any who may not get this email.

Come to Georgia and experience

Histotechnology - Southern Style


Shirley A. Powell, HT(ASCP)HTL, QIHC
Technical Director
Histology Curricular Support Laboratory
Mercer University School of Medicine
1550 College Street
Macon, GA  31207
478-301-2374 Lab
478-301-5489 Fax

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[Histonet] RE: Tissue Loss

[Shirley A. Powell]

If she is using hand lotion and handles the slides, that will make the come 
off.  She needs to take all the lotion off before cutting slides.  
Shirley

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith
Sent: Monday, December 05, 2011 9:24 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Loss

I would like some input on a situation I am faced with in our lab. We
are constantly having tissue coming off the slide on GI bx's. This is
coming from one tech, she is a senior tech and takes offense to
questioning her about the problem. From what I can tell all of the techs
are following the same protocal.
 
Judith Gale Pardue HT(ASCP), QIHC
Histology Supervisor
423-495-5756
judith_par...@memorial.org
Memorial Healthcare System
 
To the world I'm one, to one I'm the world
 

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[Histonet] RE: Unsubscribing from Histonet

[Shirley A. Powell]

Thank you Linda for providing us with such a wonderful tool for the histology 
community. I am sure all on the list appreciate it as well. 

Shirley Powell

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Linda Margraf
Sent: Friday, November 11, 2011 3:01 PM
To: 'Histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Unsubscribing from Histonet

Dear Histonetters:

Hi, I haven't put out many messages on the list recently.  I have just been 
quietly administering the list (and occasionally removing people who ask to be 
unsubscribed as my time allows).   By the way,  Histonet is coming up on its 
16th year anniversary (wow!) and  there are currently 3543 members on the list 
(from 30 countries as of my last tally).

As it keeps coming up,  if you wish to unsubscribe or modify your account 
options,  go to this link:


http://lists.utsouthwestern.edu/mailman/options/histonet

You will need to request your password if you don't know or remember it.  I 
tried to get this link added to the bottom of all the messages but apparently 
that is not possible with the current software.  If you have trouble getting 
off the list, let me know.If your email address has been changed at your 
organization, you may have trouble posting messages or unsubscribing. If so, 
let me know (but provide me with the old address that is on the membership 
list) and I'll try to help.
Thanks,
Linda M
Histonet administrator

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RE: [Histonet] Ann Preece (was decal [sic] question)

[Shirley A. Powell]

Oh I had a 1965 edition, but someone borrowed it and never brought it back.  
I have since acquired the 1972 edition in good shape, but there was a 1959 
edition.  That would be a good one to have in the library.

Shirley

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne
Sent: Tuesday, October 04, 2011 12:24 PM
To: 'Bob Richmond'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Ann Preece (was decal [sic] question)

I also have a copy of Preece's bible from 1972!  A couple of pages are loose 
and the spine is a bit frayed.  What a great book!!

Lynne Bell, HT (ASCP)
Histology Team Leader
Central Vermont Medical Center
130 Fisher Road
Berlin, VT  05641
802-371-4923

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Tuesday, October 04, 2011 11:39 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ann Preece (was decal [sic] question)

Bernice Frederick HTL (ASCP), Senior Research Tech at the  Pathology
Core Facility of the  Robert. H. Lurie Cancer Center at Northwestern
University in Chicago notes

Ann Preece states acid decal uses aqueous solutions of either formic, nitric, 
or trichloroacetic acid. Other methods mentioned are Ion-exchange resin, 
electrical ionization and chelation. The histo bible!

You've got to be almost as geezer as me to remember when Ann Preece's
A Manual for Histologic Technicians was the histo bible. I was
fortunate to be able to purloin a pristine (no stain spills) copy of
the third edition (1972) from the wreckage of an old histology lab
about 20 years ago.

Indeed, Patsy Ruegg! Decal is a trademark of the Decal Chemical
Corporation and should not be used generically for decalcifying
solutions. See decal-bone.com

Bob Richmond
Samurai Pathologist
Knoxville TN

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[Histonet] RE: I'm so sad for AFIP to close

[Shirley A. Powell]

I know, just breaks your heart.  I spent 3 weeks there in 19.. well a long 
time ago. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Friday, September 09, 2011 10:34 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] I'm so sad for AFIP to close

I never really thought it would happen but it has...

http://www.ascp.org/MainMenu/pathologists/AFIP-Closure-Major-Loss-to-Pathology-Community.html



Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax


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[Histonet] RE: Decal for IHC

[Shirley A. Powell]

5% formic acid

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laudon, Heather 
M
Sent: Friday, September 09, 2011 11:23 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Decal for IHC

Can anyone offer a recommendation for a good decalcifier that does not 
comprimise Immunohistochemistry results...in particular ER/PR? Thanks for your 
help!



Heather Laudon, HTL

Histology Technician

Surgical Pathology Laboratory

Gundersen Lutheran Hospital

1900 South Avenue

Lacrosse, WI 54601

Mail Stop: H04-008

(608)775-3139


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[Histonet] RE: Floater Problem

[Shirley A. Powell]

There is a good article in the JOH, June 2011, Vol 34, Number 2,  Gadgets for 
Floaters prevention in the Histopathology Laboratory by Izak Dimenstein that 
addresses ways to help prevent them.  He discusses floater sources in the 
discussion.

Shirley

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Friday, September 09, 2011 11:41 AM
To: histonet@lists.utsouthwestern.edu
Cc: Debra Cobb
Subject: [Histonet] Floater Problem

I am starting a PI project concerning floaters on slides.  I am
collecting data and determining whether the floater is in the block or
on the slide.  If the floater is in the block, it either came from the
grossing step or the embedding step.  Of course, both the PA and the
embedders swear that they clean their forceps and their work surfaces
diligently.  My problem is in determining where the floater actually
came from.  Any suggestions??

 

Thanks,

Laurie Colbert

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[Histonet] GSH 2012 meeting

[Shirley A. Powell]

Hi Everyone,
Just wanted to let you know we have a great lineup of speakers for the Georgia 
Society for Histotechnology meeting in 2012 when we are hosting the Region III. 
 It will be at Callaway Gardens, the Mountain Creek Inn in Pine Mountain GA 
April 13-15th.  The program will be finalized soon and up on our website at 
www.histosearch.com/gshhttp://www.histosearch.com/gsh very soon.  The flyer 
with the hotel information is already there except for the GSH group #.  I urge 
you to make your reservations now, the sooner the better, at 1800-225-5292.  
Spring is a popular time at Callaway and the rooms fill up fast.  Be sure to 
use the GSH group # 78K711 to get the discounted room rate of $109.  The price 
of your room will get you access to the gardens.  Your credit card will NOT be 
billed until you register at the hotel in April 2012.  There are only a certain 
number of rooms blocked so don't delay.

Our speaker lineup to date is below.  This is a great way to get your CEUs and 
also to visit a wonderful southern jewel.  There is something for everyone and 
April will be a gorgeous month for our meeting.  The gardens are in bloom, the 
golf courses are outstanding and there is a beach for the kids.  The butterfly 
pavilion is always a big hit with everyone.

To date our speakers and topics are:
Taiquanda Winbush-  HT/HTL Review
Adrianna Eaton -  Antibody Challenge
Claudia Lawson - Advances in Molecular Testing
Joyce Weems -  CPT Coding - Sweet Dream or Nightmare?
Marvin Hanna  -  The Molecular Pathology Lab of the Future
 Ada Feldman  -  Troubleshooting Hematoxylin and Eosin Staining
Wanda Jones  - IHC Controls---The Good, The Bad and The Ugly!
Ada Feldman  - The Joy of Histology or What We Can Learn from the Kitchen
Steven Westra  - Get the most out of your Immunohistochemistry: A Balance 
between Convenience, Cost, Education and Flexibility
Lamar Jones  -  Real Time Rapid Tissue Processing
Ely Klar - Human Tissues: Histological Identification of the Different Types

So come to Georgia in April 2012, it is going to be a Histo-terrific Meeting.

Shirley A. Powell, HT(ASCP)HTL, QIHC
GSH Secretary


478-301-2374 Lab
478-301-5489 Fax

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RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin

[Shirley A. Powell]

http://www.naacls.org/docs/Section3_HT.pdf

NAACLS spells it out.  Online Schools should be following the guidelines.  If 
not they need to rethink their programs if they are approved by NAACLS.

sp

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pam Marcum
Sent: Wednesday, August 31, 2011 12:02 PM
To: Candice Smoots
Cc: Histonet
Subject: Re: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a 
Pin



I have followed this for a while and was wondering why no one is bringing up 
the lack of Histology Schools and the small number of graduates they can 
produce per year as an issue.  Online is fine if you have a lab to work in.  
However; one of the people no longer at this facility simply did his book work 
and an HT then offered himself as just that. He never cut a section or did a 
stain yet he passed so it is possible.



Many of us are training OJTs again due to the lack of available people and the 
in our case the starting salary is low.  It is said it is because we are not 
required to complete a BS for an HT only the HTL .  It means we are doing as 
much as we can to train someone in only one lab with the small amount of time 
an understaffed Histology Laboratory has yet still have the best training for 
the field.  We are not recognized fully as Laboratory Professionals yet so it 
is not really getting any better than it was years ago.  Now we require a 2 
year degree for education in science and not much help from the organizations 
that rule us beyond pay more money.  Many of us are not able to go to meetings 
( especially NSH ) due to costs to us personally as the hospitals and 
Universities are not paying for travel and very little for educational help.



Pam Marcum













- Original Message -




From: Candice Smoots   candice _ camille @yahoo.com
To:  Histonet   histonet @lists. utsouthwestern . edu 
Sent: Wednesday, August 31, 2011 10:46:04 AM
Subject: Re: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical - To 
sticka Pin

If my memory severs me correctly.  is not required that a canidate must 
also have lab experience inorder to take the exam wheather it be from a 
histotech program or on the job training. So anyone who becomes HT/ HTL 
certified should not only know basic theory but should also have atleast basic 
experience in micotomy , staining, fixation etc. I thought that this was the 
case for any of the routes that it takes to become certified.

What I have found is that a person can start out in a specialty lab and work 
thier way up  and get the work experience that is required to sit for the exam 
however because they only have experience in THAT lab, they are in a 
disadvantage because they do not have routine experience.

For example, I know a person who started out in  a research lab and was trained 
and worked thier way up in about 3 years. They studied and sat for the exam 
because they had the required work experience and passed. They are now 
certified. However, whenn they got employed at a hospital that mostly did 
routines, they did have to be trained a little to undertsand rouine histology. 
They had never done basic trichromes  and they were slow at sectioning because 
speed was not as important where they were from. But the basic theory and 
micotmy , he had.

I say all this to say.. Just because a person has the credientials does not 
mean that they have all the experience even though they are certified. So 
sometimes it may take a little training but i admit that it shouldnt take much 
as if they are a beginner... as they are certified. Thats just my opinion.

I remain yours truely ,

Candice Camille

From: Jennifer MacDonald  JMacDonald @ mtsac . edu 
To: Rene J Buesa  rjbuesa @yahoo.com
Cc:  histonet @lists. utsouthwestern . edu   histonet @lists. utsouthwestern 
. edu ; Matthew Lunetta  MLunetta @ luhcares .org; JoyceWeems  JWeems @ 
sjha .org; histonet -bounces@lists. utsouthwestern . edu ; Shirley A. Powell 
POWELL_SA@mercer. edu 
Sent: Wednesday, August 31, 2011 10:21 AM
Subject: RE: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical - To 
stick a Pin

Graduating students in histotech programs DO clinical rotations.  They
have hands-on experience.  They are not just learning the theory from
books.




Rene J Buesa  rjbuesa @yahoo.com
Sent by: histonet -bounces@lists. utsouthwestern . edu
08/31/2011 07:54 AM

To
Shirley A. Powell POWELL_SA@mercer. edu , JoyceWeems  JWeems @ sjha .org,
Matthew Lunetta  MLunetta @ luhcares .org,
 histonet @lists. utsouthwestern . edu   histonet @lists. utsouthwestern . 
edu ,
JoeGalbraith joseph-galbraith@ uiowa . edu 
cc

Subject
RE: [ Histonet ] Re: peggy wenk comments on HT/ HTL practical - To sticka
Pin






Regardless of all the reasoning and good intentions, or even advantages
eliminating the practical part of the ASCP (either HT or HTL ) and
concentrating

RE: [Histonet] Re: peggy wenk comments on HT/HTL practical

[Shirley A. Powell]

Not a Geek, just a good teacher.  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Monday, August 29, 2011 10:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: peggy wenk comments on HT/HTL practical

I really appreciate Peggy Wenk's analysis of the practical examination
and why it had to be dropped. I never really understood the issue
before.

I must confess I always enjoyed helping the prospective examinee
obtain exactly the right tissue.

No, this endometrium is poorly preserved. We'll arrange with surgery
for a completely fresh specimen - I'll block it initially for the
diagnosis, then we'll fix it overnight and then block it exactly to
specifications. - Ick - this one's been curetted - we'll get another
one.

I'll block the margins of this colon resection specimen, then we'll
pin a portion of tissue onto paraffin and fix it flat overnight.

Next time I do an autopsy we'll get a lumbar spinal cord in the
intact dura. I'll open the dura dorsally and ventrally with iridectomy
scissors, then we'll hang it in neutral buffered formalin for two
days. Then I'll tie the dura and dependent nerves with a cotton
string. When you embed you'll remove the string, taking care that dura
and nerves remain in position. After that it's all yours. If it
doesn't work the first time, we've got three more levels in the jar.

OK, I'm a geek, I'm 72 years old, I got a right.

Bob Richmond
Samurai Pathologist
Knoxville TN

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RE: [Histonet] Slides

[Shirley A. Powell]

I use StayOn from Leica(Surgipath) which works really well on non-coated 
slides.  I cut autopsy material, unusually large sections of bloody, dried, etc 
tissue and they stay on with StayOn.  Gee I made a rhyme.  

Shirley

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas
Sent: Tuesday, August 30, 2011 7:36 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slides

Hi all! We are having issues with the tissue falling off of our slides during 
routine
staining. The tissue primarily lifts from the bottom sections on the slides. We 
have
had our stainer checked and have checked ourselves, the oven appears to be
working properly and the water pressure is fine. I tried two different slide 
manufacturers, both silane coated, and are getting the same result. Not sure 
where to go at this point except to try anon-coated slide. Any suggestions on 
a manufacturer or vendor??
Thanks in advance. Your help is always appreciated!
 
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
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RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin

[Shirley A. Powell]

I second that Joyce.
sp


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce 
[jwe...@sjha.org]
Sent: Tuesday, August 30, 2011 6:17 PM
To: Matthew Lunetta; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a 
Pin

I don't understand how a student of any program would have not a portion of 
their program dedicated to these skills. We partner with Darton College and 
their students to do a certain number of hours for their Clinicals. They know 
how to do those things, are trained by the clinical coordinator for the 
program, and are graded on their work.

Are they prepared to go into a lab and work like they've done OJT for 1-2 
years? Not at all, but they need to be hired with the understanding that they 
will need time and patience to develop their speed and their skill.

My 2 cents...


Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta
Sent: Tuesday, August 30, 2011 13:59
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin

Hey all,

I found Peggy's comments on why the practical was discontinued to be very 
interesting. Of late I have had some experience with a new HT that graduated 
from a program and passed the current HT exam.
So, as they say in Great Britain, to stick a pin in the ASCP reasons.

This new fresh and shiny HT has all the book knowledge we needed them to have. 
What they did not have was any technical skills.
1) never used a microscope or centrifuge.
2) no special staining experience
3) no embedding experience
4) no cutting experience

When they cut or embed they are no were near the speed, accuracy or quality 
that is needed in our industry. While they can answer any question you ask them 
they just do not have the technical skills one would expect from a new graduate.

I have learned several lessons from this experience.

1) I am so very glad I was one of the last HT's to have taken the practical
2) Any new HT's will be taking a practical if I am involved in the selection 
process.
3) I will question they quality of any new HT from this particular program

While I am sure that there are many new HT's that do have the skills needed, 
this one experience has caused me to be more cautious.

Respectfully,

Matt Lunetta
BS, HT (ASCP)




Message: 2
Date: Tue, 30 Aug 2011 18:09:46 +0200
From: Gudrun Lang
Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical
To: 'Bob Richmond'
Cc: histonet@lists.utsouthwestern.edu
Message-ID: 8b7976b131854abc8db236fab5026...@dielangs.at
Content-Type: text/plain; charset=iso-8859-1

Dear Dr. Richmond
Here in Austria we have a job open for a pathologist with 5 years
experience. ;)
Please, think it over to come. Lovely mountains, lovely techs...

It sounds, like you are from that sort of pathologist techs dream of.
Gudrun


-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Bob
Richmond
Gesendet: Dienstag, 30. August 2011 04:43
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical

I really appreciate Peggy Wenk's analysis of the practical examination
and why it had to be dropped. I never really understood the issue
before.

I must confess I always enjoyed helping the prospective examinee
obtain exactly the right tissue.

No, this endometrium is poorly preserved. We'll arrange with surgery
for a completely fresh specimen - I'll block it initially for the
diagnosis, then we'll fix it overnight and then block it exactly to
specifications. - Ick - this one's been curetted - we'll get another
one.

I'll block the margins of this colon resection specimen, then we'll
pin a portion of tissue onto paraffin and fix it flat overnight.

Next time I do an autopsy we'll get a lumbar spinal cord in the
intact dura. I'll open the dura dorsally and ventrally with iridectomy
scissors, then we'll hang it in neutral buffered formalin for two
days. Then I'll tie the dura and dependent nerves with a cotton
string. When you embed you'll remove the string, taking care that dura
and nerves remain in position. After that it's all yours. If it
doesn't work the first time, we've got three more levels in the jar.

OK, I'm a geek, I'm 72 years old, I got a right.

Bob Richmond
Samurai Pathologist
Knoxville TN

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RE: [Histonet] AMPHYL

[Shirley A. Powell]

Google Lysol IC and you will get plenty of suppliers.
sp

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pam Marcum
Sent: Friday, August 26, 2011 9:39 AM
To: Rae Staskiewicz
Cc: histonet@lists.utsouthwestern.edu; Barbara Crill; Sebree Linda A
Subject: Re: [Histonet] AMPHYL


We can't find Lysol IC here either so any supplier would help and to keep costs 
down a store would be better than a scientific supplier it possible.  Please id 
anyone knows let us all know as it is not easy to find. 



Pam Marcum 










- Original Message -

From: Rae Staskiewicz raest...@grics.net 
To: Sebree Linda A lseb...@uwhealth.org, Barbara Crill 
barbara.cr...@lpnt.net, histonet@lists.utsouthwestern.edu 
Sent: Thursday, August 25, 2011 4:53:36 PM 
Subject: RE: [Histonet] AMPHYL 

Linda, 

Where do you get your Lysol IC? 

Rae Staskiewicz 

-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda 
A 
Sent: Thursday, August 25, 2011 1:07 PM 
To: barbara.cr...@lpnt.net; histonet@lists.utsouthwestern.edu 
Subject: RE: [Histonet] AMPHYL 

We use Lysol I. C.; haven't be able to get Amphyl for years. 


Linda A. Sebree 
University of Wisconsin Hospital  Clinics 
IHC/ISH Laboratory 
DB1-223 VAH 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 


-Original Message- 
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
barbara.cr...@lpnt.net 
Sent: Thursday, August 25, 2011 12:56 PM 
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] AMPHYL 

My materials management department told me that AMPHYL has been 
discontinued. 
Ventana recommends that we clean/decontaminate the Benchmark and the 
Ultras with AMPHYL. 

Has anyone else ran across this?  Has AMPHYL really been discontinued? 
Is there a substitute we can use. 

Thanks everyone! 


ANTOINETTE CRILL 
ANATOMIC PATHOLOGY 
EXT 5451 

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RE: [Histonet] MSDS binders

[Shirley A. Powell]

Our Fire Marshalls even require we have a big red sign pointing to where the 
hard copies are located.  They don't need to hunt for it in case of an event.
Shirley



From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald 
[ronald.hous...@nationwidechildrens.org]
Sent: Thursday, August 25, 2011 3:31 PM
To: 'Victor Tobias'; William
Cc: Histonet@Lists. Edu
Subject: RE: [Histonet] MSDS binders

There must be some way for the Fire Department to be able to access MSDS. As 
Victor rightly points out, if your computer system is down, that option no 
longer exists. It is not only prudent, it is mandatory for the Fire Service to 
be able to look up any chemical within your facility.

If you cannot satisfy your local fire daprtment, they will close you down 
quicker than CAP or CLIA ever could.


Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor Tobias
Sent: Thursday, August 25, 2011 3:16 PM
To: William
Cc: Histonet@Lists. Edu
Subject: Re: [Histonet] MSDS binders

  In practicality you would turn to your online version first. What happens if 
the computer system/network is down. Seems like it would be prudent to have a 
hard copy available.

Victor

Victor Tobias HT(ASCP)
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
vic...@pathology.washington.edu
206-744-2735
206-744-8240 Fax
=
Privileged, confidential or patient identifiable information may be contained 
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attachments.


On 8/25/2011 12:08 PM, William wrote:
 Not certain about CLIA, but CAP only requires immediate available access to 
 the MSDS's by any personal using chemicals. I interpret that as digital 
 copies ok, as long as everyone has access. I have passed three inspections 
 with digital MSDS only.

 Will Chappell

 Sent from my iPhone

 On Aug 25, 2011, at 11:58 AM, Jill Coxjco...@yahoo.com  wrote:

 Hello Histonetters,

 Does anyone know if there is a rule as to MSDS binders having to be in 
 yellow and black bold lettering? Do we even still need to have hard copy if 
 we have access to msds.com on desk top? I am in Ca and will be CLIA 
 licensed.. Thank you in advance!!

 Jill Cox, HT ASCP
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[Histonet] Online Histology program

[Shirley A. Powell]

Darton College, Albany GA has an online program, go to 
http://www.darton.edu/programs/AlliedHealth/hist/ then click on the HT 
navigation to the right.   


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ompoc, Marie
Sent: Friday, August 12, 2011 12:01 PM
To: 'Connolly, Brett M'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: umbilical cord block

Hi all,

I'm interested to know about this online histology program? Can anybody give me 
the site? Thank you all!

Thank You,
Christine


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett 
M
Sent: Friday, August 12, 2011 8:37 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] umbilical cord block

Hi all,

Would anyone be willing to donate an FFPE block of human umbilical for a 
student I am mentoring who enrolled in an on-line histology training program?

Thanks,
Brett

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





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RE: [Histonet] Re: Knife for trimming paraffin from blocks

[Shirley A. Powell]

I too use the paratrimmer.  It is great for avoiding repetitive injuries.  For 
those of us who have carpal tunel syndrome and arthritis in the hands and 
cannot hold a small knife long enough to scrap multiple blocks it is great.  
Just part of my PPE.  Worth every penny.
Shirley

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cormier, 
Kathleen
Sent: Monday, August 08, 2011 11:04 AM
To: gayle callis; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Knife for trimming paraffin from blocks

We use the paratrimmer too. What we do for a paraffin catcher is to use
a specimen cup (I call them urine cups, but whatever) to catch the
paraffin. When it get fullish, we place in the slide oven to melt the
paraffin, when melted pour off, and reuse the cup... :)

Kathy Cormier
Histology Manager
Charles River Laboratories
251 Ballardvale Street 
Wilmington, MA 01887
Ph: 781-222-6803
Fax: 978-988-8793
kathleen.corm...@crl.com
Accelerating Drug Development. Exactly.
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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of gayle
callis
Sent: Monday, August 08, 2011 10:56 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Knife for trimming paraffin from blocks

You Wrote: 

 

I am looking for a stainless steel knife that we use to scrape off the 

paraffin from the embedded blocks.  I could not find the 6 inches size 

knife in any catalog. 

 

Thanks in advance

 

Mala

 

Nirmala Srishan

Histology Supervisor

Holy Name Medical Center.

 


*

Dear Mala, 

 

In the past, we used an old style permanent edge scalpel blade but after
a
disaster with one person seirously cutting himself while trimming
paraffin
from a block, we purchased a Paratrimmer with a heated, slanted metal
surface.  This trimmer has made everyone happy by eliminating the
potential
for serious injury and does an even better job of getting rid of excess
paraffin. 

 

There are two models sold, one from Thermo Scientific and the other one
was
recently spotted on a vendor website. (Sorry, I didn't jot the name
down).
The trimmers  are worth the investment to keep you and your employees
safe
from nasty cuts, no matter what the knife/blade, etc could be used.  

 

The only drawback to the Para Trimmer is the messy paraffin drippings
have
to be collected in some separate container that the manufacturer doesn't
supply.  This is more than a bit annoying.  It seems to me the
manufacturer
of these devices would design the trimmer with a paraffin catch tray.  A
small aluminum baking pan (from grocery store) could be used and
disposed of
(not a Green consideration).

 

Be safe rather than sorry.  

 

Gayle M. Callis 

HTL/HT/MT(ASCP) 

 

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RE: [Histonet] knife used for cleaning paraffin off the blocks

[Shirley A. Powell]

I use a glass staining dish to catch the drippings.  When full I place it in 
the freezer to harden and it comes right out and I toss it.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
MacDonald
Sent: Monday, August 08, 2011 11:30 AM
To: Grantham, Andrea L - (algranth)
Cc: HISTONET; histonet-boun...@lists.utsouthwestern.edu
Subject: Re: [Histonet] knife used for cleaning paraffin off the blocks

If you put a piece of paper towel in the bottom of the drip catcher  you 
just change that and it makes it much easier.




Grantham, Andrea L - (algranth) algra...@email.arizona.edu 
Sent by: histonet-boun...@lists.utsouthwestern.edu
08/08/2011 08:28 AM

To

cc
HISTONET histonet@lists.utsouthwestern.edu
Subject
Re: [Histonet] knife used for cleaning paraffin off the blocks






Actually we have a ParaTrimmer too - it was not that expensive and we love 
it. We paid @ $500 for ours and I see the they are a bit more expensive 
now - list price anyway.
To solve the drip problem I put one of the tops from the boxes of pipette 
tips under the ledge and it catches all the drips. When the paraffin 
hardens you can just pop it out and when the drip catcher gets nasty we 
just throw it away and get another one.



On Aug 8, 2011, at 8:13 AM, Grantham, Andrea L - (algranth) wrote:

 Don't look in a catalog - go to Target or WalMart and look on the wall 
with kitchen gadgets. It will be much cheaper.
 
 
 
 On Aug 8, 2011, at 6:28 AM, sris...@mail.holyname.org wrote:
 
 Hi All,
 
 I wonder if someone could help me with this!
 
 I am looking for a stainless steel knife that we use to scrape off the 
 paraffin from the embedded blocks.  I could not find the 6 inches size 
 knife in any catalog. 
 
 Thanks in advance
 
 Mala
 
 Nirmala Srishan
 Histology Supervisor
 Holy Name Medical Center.
 
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[Histonet] histology equipment service

[Shirley A. Powell]

For the person/persons looking for someone to do equipment maintenance/service 
I can highly recommend this company, Pathology Service, Inc.  Sean Draime and 
Joel DeVisser are very knowledgeable on all types of histology equipment and 
their charges are reasonable.  They are in Georgia, of course, GO DAWGS, but 
travel all over the country doing repair, PM, and also have used equipment now. 
 Their number is 1-866-398-9478, or contact them by going to 
www.pathologyserv.comhttp://www.pathologyserv.com.  Also see email below for 
more information on equipment purchases.


To: Shirley A. Powell
Subject: Joel from PSI

Hi, this is my email here for any info you come across reference people who may 
need to sell any lab equipment or labs.  We also do offer very competitive 
service and preventative maintenance packages and cover most of the USA.  Sean 
has 3 different plans that can be customized to fit any clients needs or 
budgets.  We also sell used, refurbished equipment and our pieces go through 
frame off restorations and come out working and looking like new again.  We 
offer 90 day warranties on everything we sell and 1 year extensions all through 
PSI.  Thank you so much for the information on the news letter and for taking 
the time to speak with me earlier on the phone.

Joel DeVisser
678-887-6068
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[Histonet] FW: histology equipment service

[Shirley A. Powell]

Okay the website is www.pathologyserv.com for some reason it was duplicated.  
On the site it focuses on eastern states but they have expanded to cover most 
of the USA.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. 
Powell
Sent: Thursday, August 04, 2011 5:14 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] histology equipment service

For the person/persons looking for someone to do equipment maintenance/service 
I can highly recommend this company, Pathology Service, Inc.  Sean Draime and 
Joel DeVisser are very knowledgeable on all types of histology equipment and 
their charges are reasonable.  They are in Georgia, of course, GO DAWGS, but 
travel all over the country doing repair, PM, and also have used equipment now. 
 Their number is 1-866-398-9478, or contact them by going to 
www.pathologyserv.comhttp://www.pathologyserv.com.  Also see email below for 
more information on equipment purchases.


To: Shirley A. Powell
Subject: Joel from PSI

Hi, this is my email here for any info you come across reference people who may 
need to sell any lab equipment or labs.  We also do offer very competitive 
service and preventative maintenance packages and cover most of the USA.  Sean 
has 3 different plans that can be customized to fit any clients needs or 
budgets.  We also sell used, refurbished equipment and our pieces go through 
frame off restorations and come out working and looking like new again.  We 
offer 90 day warranties on everything we sell and 1 year extensions all through 
PSI.  Thank you so much for the information on the news letter and for taking 
the time to speak with me earlier on the phone.

Joel DeVisser
678-887-6068
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[Histonet] Brady labeling system

[Shirley A. Powell]

I would like to talk to anyone who uses the Brady Specimen labeling system for 
cassettes and slides.  Please contact me via this email address or by phone 
number below.  Thanks for your help in advance.

Shirley

Shirley A. Powell, HT(ASCP)HTL, QIHC
Technical Director
Histology Curricular Support Laboratory
Mercer University School of Medicine
1550 College Street
Macon, GA  31207
478-301-2374 Lab
478-301-5489 Fax

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[Histonet] Diff-Quik stain

[Shirley A. Powell]

Hi Guys,

I am looking for diff-quick stain kits that are economical.  My pathologist is 
contemplating another medical mission trip to Haiti and I need to locate a kit 
that she can take with her for blood smears.  I have found a few upwards of 
$600 but she does not need a gallon of each of the solutions.  If you know of a 
supplier of smaller volumes or even if you have protocols where you make your 
own, please share.

Vendors comments are welcome if you contact me personally, please do not flood 
histonet with ads.

Thanks in advance.

Shirley

Shirley A. Powell, HT(ASCP)HTL, QIHC
Technical Director
Histology Curricular Support Laboratory
Mercer University School of Medicine
1550 College Street
Macon, GA  31207
478-301-2374 Lab
478-301-5489 Fax

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RE: [Histonet] National Average Salary

[Shirley A. Powell]

Oh I think you will find that 98% of us are underpaid.  :)

-Original Message-
From: sgoe...@mirnarx.com [mailto:sgoe...@mirnarx.com] 
Sent: Thursday, March 31, 2011 10:48 AM
To: Shirley A. Powell; lbustama...@cvm.tamu.edu
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] National Average Salary

So apparently I'm underpaid =)  Good Luck convincing employers of this!

Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley
A. Powell
Sent: Thursday, March 31, 2011 9:35 AM
To: Lin Bustamante
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] National Average Salary

Salary survey is in the March 2011 issue of LabMedicine from ASCP.  You
can also get it from www.ascp.org web site.  




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lin
Bustamante
Sent: Thursday, March 31, 2011 10:30 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] National Average Salary

Can anyone please send me the National Average Salary chart for
Histotechnician and HT Supervisor with more than 5 years experience?
Thank you very much.
Lin Bustamante

Lin S. Bustamante, B.Sc.; HT(ASCP)
Research Associate
Histology Lab Supervisor
Veterinary Integrative Bioscience
Texas AM University
College Station, TX 77843-4458
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RE: [Histonet] Grossing Station Recommendation

[Shirley A. Powell]

Try Mopec.

http://www.mopec.com/category/5/grossing_stations__workstations/


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sean McBride
Sent: Monday, March 21, 2011 2:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Grossing Station Recommendation

Colleagues,

We are looking to replace our current pathology grossing hood with a new 
station, so I am looking for recommendations.  Thanks in advance for all of 
your wonderful advice.


Best regards,


~Sean McBride


Scientific Specialist
Bone Tissue Engineering Center
Carnegie Mellon Research Institute
Suite 4311
700 Technology Drive
Pittsburgh, PA 15219-3124

412-268-8275 (o)
412-915-1683 (m)
412-268-8275 (fax)
smcbr...@andrew.cmu.edu 






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[Histonet] GSH meeting reminder

[Shirley A. Powell]

Hi Everyone, this is the last time I promise this year, but  the GSH meeting is 
coming up this weekend.  You can still register for the workshops/lectures, and 
the HT/HTL review on Friday at 1, awards luncheon on Saturday and the general 
membership meeting, vendor exhibits reception on Friday night, and exhibits all 
day Saturday.  Come and connect with old friends.  We are expecting a wonderful 
meeting, wonderful weather and hope you can join us.

I have attached a copy of the program for those of you who were uncertain that 
you could make it.  Come to Callaway Gardens at Pine Mountain, Georgia for a 
great meeting.  You can find more information at 
www.histosearch.com/gshhttp://www.histosearch.com/gsh and click on symposium 
tab at the bottom.  Vendor registration forms can be downloaded from there as 
well.

Shirley Powell
GSH Secretary



Shirley A. Powell, HT(ASCP)HTL, QIHC
Technical Director
Histology Curricular Support Laboratory
Mercer University School of Medicine
1550 College Street
Macon, GA  31207
478-301-2374 Lab
478-301-5489 Fax

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[Histonet] GSH Annual Meeting at Callaway Gardens, Georgia

[Shirley A. Powell]

Hi Histo Friends,

Our meeting is only 3 weeks away, make your plans if you have not already done 
so.


You can still register for the meeting right up until it begins, but make your 
hotel reservations now before the price goes up.  Location is The Mountain 
Creek Inn, Callaway Gardens, Pine Mountain, Georgia, call for hotel 
reservations at 1-800-225-5292 before all the discounted rooms are gone.  Also 
go to www.histosearch.com/gshhttp://www.histosearch.com/gsh and on the 
symposium page download your program.  Vendors can find their registration form 
on the same page.



Room rates start at $99 (IF ROOMS ARE STILL AVAILABLE) plus tax which includes 
Continental Breakfast and Admission to the Park.  For more information about 
things to do at Callaway click on this link:   
http://www.callawaygardens.com/resort/things-to-do/georgia-fun.aspx



Our theme this year is METAMORPHOSIS:  Transforming Histotechs.



If anyone has questions, please contact me for assistance.



Come TRANSFORM yourselves.





Shirley Powell

GSH Secretary



Shirley A. Powell, HT(ASCP)HTL, QIHC
Technical Director
Histology Curricular Support Laboratory
Mercer University School of Medicine
1550 College Street
Macon, GA  31207
478-301-2374 Lab
478-301-5489 Fax

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RE: [Histonet] How many tissues an histo tech is suppose to cut per hour?

[Shirley A. Powell]

Bet you he already does.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Tuesday, March 01, 2011 3:01 PM
To: 'Marcum, Pamela A'; 'Jay Lundgren'; Mahoney, Janice A
Cc: histonet@lists.utsouthwestern.edu; Jenny Vega
Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per 
hour?

I hope the whole paragraph was supposed to be humor or Jay may have a lynch mob 
at his door!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A
Sent: Tuesday, March 01, 2011 3:00 PM
To: 'Jay Lundgren'; Mahoney,Janice A
Cc: histonet@lists.utsouthwestern.edu; Jenny Vega
Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per 
hour?

I truly hope that was humor about oversupply.  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren
Sent: Tuesday, March 01, 2011 1:35 PM
To: Mahoney,Janice A
Cc: histonet@lists.utsouthwestern.edu; Jenny Vega
Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per 
hour?

  30 blocks/hour, including rough cutting, is the standard.  That's
supposing a few (5-10) levels, specials, etc.
If I am cutting all big tissue, what I've heard called onesies (just one
slide each), I can  cut 60 blocks/hour.
If one is cutting a tray full of persnickety prostate (with 6 levels on one
slide), renal, and bone marrow bxs with tons of specials and immunos, well,
that is going to slow you down.


  Anyway, I disagree with those who say speed isn't as important as quality,
patience, etc.  I think new histotechs should have someone with a stopwatch
and a clipboard standing behind them while they're cutting, yelling Faster,
faster in their ear.  If anyone cannot measure up to a strict quota
they should be immediately dismissed.  We have such a disgusting oversupply
of new Histotechnology students as it is. ;)

Jay A. Lundgren
M.S., HTL(ASCP)
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[Histonet] Deadline extended

[Shirley A. Powell]

Hi Everyone,

Good news, the hotel extended the deadline for the GSH discount rate.  As long 
as they have rooms they will honor the $99 @ night rate that includes 
continental breakfast and entrance to the park. There is no deadline for 
registering for the meeting, but for us to have name tags ready for you, please 
register beforehand.


Information for the meeting.



The Georgia Society for Histotechnology invites you to our meeting March 25-27, 
2011 at Callaway Gardens in Pine Mountain, Georgia which is near Columbus, Ga. 
and very convenient to Alabama folks, so come across the line.  The invitation 
extends to any other states as well.  Callaway Gardens is a fantastic site for 
family vacations, golf lovers, nature lovers, so come to Georgia for a visit 
and take in a wealth of histology knowledge. The Mountain Creek Inn, Callaway 
Gardens, Pine Mountain, Georgia is the location and you can call for hotel 
reservations at 1-800-225-5292.  Room rates start at $99 which includes 
Continental Breakfast and Admission to the Park.  For more information about 
things to do at Callaway click on the link here:   
http://www.callawaygardens.com/resort/things-to-do/georgia-fun.aspx



Our theme this year is METAMORPHOSIS:  Transforming Histotechs.  The complete 
program can be downloaded from our website at this link:  
www.histosearch.com/gshhttp://www.histosearch.com/gshhttp://www.histosearch.com/gsh%3chttp:/www.histosearch.com/gsh
 then click on GSH symposium link at the bottom of the home page.  There you 
will find the complete program with registration form on page 4.  The vendor 
registration form is on the same page for any last minute vendors who want to 
exhibit at our meeting.



If anyone has questions, please contact me for assistance.



Come TRANSFORM yourselves.



Shirley Powell

GSH Secretary


Shirley A. Powell, HT(ASCP)HTL, QIHC
Technical Director
Histology Curricular Support Laboratory
Mercer University School of Medicine
1550 College Street
Macon, GA  31207
478-301-2374 Lab
478-301-5489 Fax

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[Histonet] HURRY the GSH meeting deadline for hotel reservations is TOMORROW

[Shirley A. Powell]

Reminder for ALL histotechs,



Last day before deadline at the Mountain Creek Inn tomorrow, the 23rd.  The 
actual meeting registration has no deadline, but the prices will go up at the 
hotel and rooms are going fast.  Call today, and definitely by tomorrow.



The Georgia Society for Histotechnology invites you to our meeting March 25-27, 
2011 at Callaway Gardens in Pine Mountain, Georgia which is near Columbus, Ga. 
and very convenient to Alabama folks, so come across the line.  The invitation 
extends to any other states as well.  Callaway Gardens is a fantastic site for 
family vacations, golf lovers, nature lovers, so come to Georgia for a visit 
and take in a wealth of histology knowledge.



The deadline for making hotel reservations is tomorrow, Feb 23, 2011  so that 
gives you a day to make your plans to attend, don't delay.  The Mountain Creek 
Inn, Callaway Gardens, Pine Mountain, Georgia is the location and you can call 
for hotel reservations at 1-800-225-5292.  Room rates start at $99 which 
includes Continental Breakfast and Admission to the Park.  For more information 
about things to do at Callaway click on the link here:   
http://www.callawaygardens.com/resort/things-to-do/georgia-fun.aspx



Our theme this year is METAMORPHOSIS:  Transforming Histotechs.  The complete 
program can be downloaded from our website at this link:  
www.histosearch.com/gshhttp://www.histosearch.com/gshhttp://www.histosearch.com/gsh%3chttp:/www.histosearch.com/gsh
 then click on GSH symposium link at the bottom of the home page.  There you 
will find the complete program with registration form on page 4.  The vendor 
registration form is on the same page for any last minute vendors who want to 
exhibit at our meeting.



If anyone has questions, please contact me for assistance.



Come TRANSFORM yourselves.



Shirley Powell

GSH Secretary


Shirley A. Powell, HT(ASCP)HTL, QIHC
Technical Director
Histology Curricular Support Laboratory
Mercer University School of Medicine
1550 College Street
Macon, GA  31207
478-301-2374 Lab
478-301-5489 Fax

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[Histonet] Correction, Correction on cutoff date for GSH meeting

[Shirley A. Powell]

Correction: There is a correction on the cutoff date for the hotel 
reservations.  Cutoff date for discounted room rate is February 23rd, Not March 
1st.  There are several other events going on that weekend and the hotel is 
filling up fast.  Please make your reservations and register soon so you will 
not miss out on the great room rate and a great meeting.

Shirley Powell


Hi Georgia, Alabama, ALL histotechs,



The Georgia Society for Histotechnology invites you to our meeting March 25-27, 
2011 at Callaway Gardens in Pine Mountain, Georgia which is near Columbus, Ga. 
and very convenient to Alabama folks, so come across the line.  The invitation 
extends to any other states as well.  Callaway Gardens is a fantastic site for 
family vacations, golf lovers, nature lovers, so come to Georgia for a visit 
and take in a wealth of histology knowledge.



The deadline for making hotel reservations is March 1, 2011  so that gives you 
a month to make your plans to attend, don't delay.  The Mountain Creek Inn, 
Callaway Gardens, Pine Mountain, Georgia is the location and you can call for 
hotel reservations at 1-800-225-5292.  Room rates start at $99 which includes 
Continental Breakfast and Admission to the Park.  For more information about 
things to do at Callaway click on the link here:   
http://www.callawaygardens.com/resort/things-to-do/georgia-fun.aspx



Our theme this year is METAMORPHOSIS:  Transforming Histotechs.  The complete 
program can be downloaded from our website at this link:  
www.histosearch.com/gshhttp://www.histosearch.com/gshhttp://www.histosearch.com/gsh%3chttp:/www.histosearch.com/gsh
 then click on GSH symposium link at the bottom of the home page.  There you 
will find the complete program with registration form.  The vendor registration 
form is on the same page for any last minute vendors who want to exhibit at our 
meeting.  If anyone has questions, please contact me for assistance.



Come TRANSFORM yourselves.





Shirley Powell

GSH Secretary


Shirley A. Powell, HT(ASCP)HTL, QIHC
Technical Director
Histology Curricular Support Laboratory
Mercer University School of Medicine
1550 College Street
Macon, GA  31207
478-301-2374 Lab
478-301-5489 Fax

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[Histonet] Weekly reminder

[Shirley A. Powell]

Reminder to all Georgia, Alabama, ALL histotechs,



The Georgia Society for Histotechnology invites you to our meeting March 25-27, 
2011 at Callaway Gardens in Pine Mountain, Georgia which is near Columbus, Ga. 
and very convenient to Alabama folks, so come across the line.  The invitation 
extends to any other states as well.  Callaway Gardens is a fantastic site for 
family vacations, golf lovers, nature lovers, so come to Georgia for a visit 
and take in a wealth of histology knowledge.



The deadline for making hotel reservations is March 1, 2011  so that gives you 
a month to make your plans to attend, don't delay.  The Mountain Creek Inn, 
Callaway Gardens, Pine Mountain, Georgia is the location and you can call for 
hotel reservations at 1-800-225-5292.  Room rates start at $99 which includes 
Continental Breakfast and Admission to the Park.  For more information about 
things to do at Callaway click on the link here:   
http://www.callawaygardens.com/resort/things-to-do/georgia-fun.aspx



Our theme this year is METAMORPHOSIS:  Transforming Histotechs.  The complete 
program can be downloaded from our website at this link:  
www.histosearch.com/gshhttp://www.histosearch.com/gshhttp://www.histosearch.com/gsh%3chttp:/www.histosearch.com/gsh
 then click on GSH symposium link at the bottom of the home page.  There you 
will find the complete program with registration form.



The Vendor registration form is on the same page for any last minute vendors 
who want to exhibit at our meeting.  If anyone has questions, please contact me 
for assistance.



Come TRANSFORM yourselves.





Shirley Powell

GSH Secretary




Shirley A. Powell, HT(ASCP)HTL, QIHC
Technical Director
Histology Curricular Support Laboratory
Mercer University School of Medicine
1550 College Street
Macon, GA  31207
478-301-2374 Lab
478-301-5489 Fax

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[Histonet] Weekly reminder

[Shirley A. Powell]

Hi Georgia, Alabama, ALL histotechs,



The Georgia Society for Histotechnology invites you to our meeting March 25-27, 
2011 at Callaway Gardens in Pine Mountain, Georgia which is near Columbus, Ga. 
and very convenient to Alabama folks, so come across the line.  The invitation 
extends to any other states as well.  Callaway Gardens is a fantastic site for 
family vacations, golf lovers, nature lovers, so come to Georgia for a visit 
and take in a wealth of histology knowledge.



The deadline for making hotel reservations is March 1, 2011  so that gives you 
a month to make your plans to attend, don't delay.  The Mountain Creek Inn, 
Callaway Gardens, Pine Mountain, Georgia is the location and you can call for 
hotel reservations at 1-800-225-5292.  Room rates start at $99 which includes 
Continental Breakfast and Admission to the Park.  For more information about 
things to do at Callaway click on the link here:   
http://www.callawaygardens.com/resort/things-to-do/georgia-fun.aspx



Our theme this year is METAMORPHOSIS:  Transforming Histotechs.  The complete 
program can be downloaded from our website at this link:  
www.histosearch.com/gshhttp://www.histosearch.com/gshhttp://www.histosearch.com/gsh%3chttp:/www.histosearch.com/gsh
 then click on GSH symposium link at the bottom of the home page.  There you 
will find the complete program with registration form.  The vendor registration 
form is on the same page for any last minute vendors who want to exhibit at our 
meeting.  If anyone has questions, please contact me for assistance.



Come TRANSFORM yourselves.





Shirley Powell

GSH Secretary




Shirley A. Powell, HT(ASCP)HTL, QIHC
Technical Director
Histology Curricular Support Laboratory
Mercer University School of Medicine
1550 College Street
Macon, GA  31207
478-301-2374 Lab
478-301-5489 Fax

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[Histonet] GSH meeting - hotel deadline

[Shirley A. Powell]

Hi Georgia, Alabama, ALL histotechs,

The Georgia Society for Histotechnology invites you to our meeting March 25-27, 
2011 at Callaway Gardens in Pine Mountain, Georgia which is near Columbus, Ga. 
and very convenient to Alabama folks, so come across the line.  The invitation 
extends to any other states as well.  Callaway Gardens is a fantastic site for 
family vacations, golf lovers, nature lovers, so come to Georgia for a visit 
and take in a wealth of histology knowledge.

The deadline for making hotel reservations is March 1, 2011  so that gives you 
a month to make your plans to attend, don't delay.  The Mountain Creek Inn, 
Callaway Gardens, Pine Mountain, Georgia is the location and you can call for 
hotel reservations at 1-800-225-5292.  Room rates start at $99 which includes 
Continental Breakfast and Admission to the Park.  For more information about 
things to do at Callaway click on the link here:   
http://www.callawaygardens.com/resort/things-to-do/georgia-fun.aspx

Our theme this year is METAMORPHOSIS:  Transforming Histotechs.  The complete 
program can be downloaded from our website at this link:  
www.histosearch.com/gshhttp://www.histosearch.com/gsh then click on GSH 
symposium link at the bottom of the home page.  There you will find the 
complete program with registration form.  The vendor registration form is on 
the same page for any last minute vendors who want to exhibit at our meeting.  
If anyone has questions, please contact me for assistance.

Come TRANSFORM yourselves.


Shirley Powell
GSH Secretary



Shirley A. Powell, HT(ASCP)HTL, QIHC
Technical Director
Histology Curricular Support Laboratory
Mercer University School of Medicine
1550 College Street
Macon, GA  31207
478-301-2374 Lab
478-301-5489 Fax

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[Histonet] GSH meeting in March

[Shirley A. Powell]

Hi Fellow Histonetters,

The Georgia Society for Histotechnology wants to invite you to our meeting at 
Callaway Gardens, Pine Mountain GA March 25-27th, 2011.  We had our meeting 
there in 2007 which had a wonderful program and turnout.  This year should be 
the same.

Our program can be downloaded by going to
http://www.histosearch.com/gsh/symposium.html

The vendor registration form can also be downloaded here too.

Make your reservations early at the Mountain Creek Inn by calling 
1-800-225-5292.  The room rates are $99 which includes Continental Breakfast 
and Admission to the Park.

Plan to join us in the beautiful gardens and enjoy the peaceful surroundings.  
make it part of your vacation, bring the family.  The championship golf 
courses, the man-made beach, and the acres and acres of flowers, birds, plants, 
animals  and the butterfly sanctuary  are outstanding.  Check them out at their 
website http://www.callawaygardens.com/resort/things-to-do/georgia-fun.aspx 
today.


Shirley Powell, GSH Secretary




Shirley A. Powell, HT(ASCP)HTL, QIHC
Technical Director
Histology Curricular Support Laboratory
Mercer University School of Medicine
1550 College Street
Macon, GA  31207
478-301-2374 Lab
478-301-5489 Fax

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[Histonet] cheap metal detector

[Shirley A. Powell]

Here is a website for cheap metal detectors for those who need to find staples 
in specimens for their pathologists.
Shirley

http://www.woodcraft.com/Product/2003820/9451/Little-Wizard-Metal-Detector.aspx
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RE: [Histonet] Nails

[Shirley A. Powell]

Switch to Stay On and plain slides, I had problems with charged slides and now 
have good results with the additive and regular slides.  Cheaper in the long 
run too.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
godsgal...@aol.com
Sent: Friday, November 19, 2010 11:28 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Nails


We are having a very difficault time with the naisl falling off the slides when 
we do the PASF stain.  We are using charged slides already and we have tried 
increasing the baking time during drying to no avail.

Any suggestions?


Roxanne



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RE: [Histonet] Nails

[Shirley A. Powell]

So if you can't find Stay On from Surgipath/Leica now, you can make your own 
additive.  I used to make up an Agar solution, see method below, and used 20 mL 
of this to my water bath that was about 2000 mL.  

Agar, Agar (Bacto Agar)  2 gm
Distilled water800 mL
Dissolve completely in a flask using medium heat and magnetic stirrer.  Cool 
and add a few grains of thymol.  Store in the frig.  You may need to shake it 
to break it up before use, but this is what I used for years before the 
commercial stuff came out.

Shirley

-Original Message-
From: Spath, Judith [mailto:spath.jud...@marshfieldclinic.org] 
Sent: Friday, November 19, 2010 11:49 AM
To: powell...@mercer.edu
Subject: Re: [Histonet] Nails

Right now, there is a serious BACK ORDER on STA ON...just an FYI

--Original Message--
From:   Shirley A. Powell powell...@mercer.edu
Date:   Fri Nov 19, 2010 -- 10:41:30 AM
To: godsgal...@aol.com,histonet@lists.utsouthwestern.edu
Subject:RE: [Histonet] Nails

Switch to Stay On and plain slides, I had problems with charged slides and now 
have good results with the additive and regular slides.  Cheaper in the long 
run too.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
godsgal...@aol.com
Sent: Friday, November 19, 2010 11:28 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Nails


We are having a very difficault time with the naisl falling off the slides when 
we do the PASF stain.  We are using charged slides already and we have tried 
increasing the baking time during drying to no avail.

Any suggestions?


Roxanne



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[Histonet] RE: Metal detectors

[Shirley A. Powell]

A small stud finder should work.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gagnon, Eric
Sent: Thursday, November 18, 2010 12:26 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Metal detectors

This is an interesting idea, Valerie, and I too will be interested in any 
responses.  Such a detector would certainly reduce the number of I couldn't 
see the staples responses we get when we ask our Pathologist's Assistants 
about these problematic sections.  Another question I'd have is whether the 
detector can detect all sizes of surgical staples: the large ones and the 
multiple tiny ones which tend to be used in greater numbers and are harder to 
detect.
 
Now if we could only find a suture detector...
 
Thanks in advance,
 
Eric Gagnon MLT
Histology Laboratory
Kingston General Hospital,
Kingston, Ontario, Canada


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[Histonet] RE: paraffin block mailers

[Shirley A. Powell]

Hi Jeanine, 

At the NSH vendors I got a sample of a neat clear plastic holder from Source 
Medical Products, www.sourcemp.com, 1866-735-9965, holds 4 maybe 8 blocks for 
mailing, depending upon the thickness.  

Shirley


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, 
Jeanine (CDC/OID/NCZVED)
Sent: Monday, October 25, 2010 9:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] paraffin block mailers

Hi everyone,

Does anyone know of a source for packaging exclusive to mailing paraffin
blocks?  Vendors please feel free to reply as well.

Thanks!

Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590 
jeanine.bartl...@cdc.hhs.gov


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[Histonet] RE: Questions

[Shirley A. Powell]

Amy, 

You do not need to apologize for asking a question to which you did not know 
the answer.  This is an educational avenue, for histology, and there is no such 
thing as a stupid question if you need answers to solve a problem.  

Those of us who teach know questions are important, even if you think you know 
the answer but not exactly sure, or in your case you knew but needed documented 
verification from others in the field.  I hope your fellow workers and 
supervisors got the message and please feel free to ask.  There are those in 
the field who feel this is a social network for experts and that is okay too, 
but the real reason NSH and histosearch was started was to expand knowledge of 
the histology community and to improve our profession.  Remembering when 
histology was in the basement and no one knew we were there, it makes me proud 
of the progress we have made in the 48 years I have been in the field.  

Keep asking and share what you know, no need for apologies.

Shirley




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R
Sent: Monday, September 20, 2010 11:19 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Questions

I originally asked my questions because I *knew* it was being done
incorrectly and no one @ my workplace believed me when I tried to show
them the way I was taught/trained-as stated in my original post.

Regardless, it never occurred to me that my questions were something
that would be met with oh no or oh my gosh - I feel as though I
should apologize for my stupid question.

 

However, thank you, to those who responded with your procedures. I'm
making a great case based on what we know!

 

Have a good week!

 

 

Amy R. Senn

Holy Spirit Health System

503 N. 21st Street

Camp Hill, PA 17011

Phone: 717-763-2124

Fax: 717-763-2947

www.hsh.org



 

 

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RE: [Histonet] Help! In need of positive Gram Control

[Shirley A. Powell]

Go to your local drive in mart and buy you a Slim Jim, cut it in thin slices 
and you will have all the Gram controls you can use.  You will never eat 
another Slim Jim.  If you don't know what they are, they are sort of like a 
summer sausage, only thinner and guys love them.  They are found usually right 
beside the check out register.  

Shirley


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of dianar...@aol.com 
[dianar...@aol.com]
Sent: Monday, June 21, 2010 7:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Help! In need of positive Gram Control

Help! We are in need of positive Gram Control Blocks if anyone has any
extra they are willing to part with.  I have lots of Fungus, Pneumocystis  and
HPV tissue blocks to trade.

Diana Ripley
John Muir Histology
Concord Campus
2540 East Street
Concord, CA 94520
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[Histonet] Need help

[Shirley A. Powell]

Hi Histonetters,

One of my pathologists is going on a mission to Haiti in July.  She is taking 
battery powered microscope to use.  She is not sure what type of power is 
available.  All the manual says about the battery option is the LED 
configuration is operational with an in-built re-chargeable battery and 
charging circuit.  The battery will be charged with a direct input power supply 
of 110-240V AC 50Hz/g0Hz which ensures continuous operation under fluctuating 
voltages.  Greek to me.  Has anyone been down since the earthquake using such 
battery powered scope?  Know of power limitations?  If so, got any tips, clues, 
pieces of wisdom?  I know there are experts out there who know.

Thanks,


Shirley A. Powell, HT(ASCP)HTL, QIHC
Technical Director
Histology Curricular Support Laboratory
Mercer University School of Medicine
1550 College Street
Macon, GA  31207
478-301-2374 Lab
478-301-5489 Fax

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[Histonet] RE: Freezing spray artifact

[Shirley A. Powell]

There is a text written by the late Lee Luna and Samuel Wesley Thompson 
entitled An Atlas of Artifacts in this the artifact from freeze spray is 
pictured.  The ISBN # is 0-398-03624-1 Published by Charles C. Thomas, 
Publisher.  

Shirley Powell

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Erin
Sent: Wednesday, May 19, 2010 12:09 PM
To: histonet
Subject: [Histonet] Freezing spray artifact

Has anyone run into a problem or artifact from freezing spray?  I think we may 
be having a problem with it but I can't find any pictures or descriptions of 
what it looks like.

Thanks in advance,
Erin

Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248

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