[Histonet] (no subject)
Please unsubscribe me. I tried this on your site, but still receiving messages. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hard Tissue Lab
Does anyone know of a hard tissue lab that will work on outside cases? A doctor I know needs this information. Thanks *Courage without limits. Thank you to all whose loved ones fought and served for us. I will remember them. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Rolling of my ribbon ? Another paraffin question.
*I am presently using a paraffin designated as an IM product.* *We are a facility that cuts only autopsies and have been experiencing alot of rolling of our sections.* *We did recently switch to this product, because of cost.* *Question : Is the paraffin you are using working well on autopsy tissue and producing ribbons right away ? Do you use it for infiltration as well as embedding ?* *Thank you for your assistant.* *Stella Walters* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fat Stain on Paraffin Slide ?
Ok Experts - Please advise me on a stain for Fat which I can do on paraffin sections? Could you expand on your own experience with such an animal. Thanks to All Stella Houston, Tx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Who is scanning thieir HE's slides for Path.
We are gathering info. on the possibility of scanning our routine slides for our Path. If you are doing this in your lab, would you mind sharing some info. on the eqiupment you are using? Stella ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Scanning routine slides for Path.
*I need to gather information on any instrumentation that will enable me to digitally scan routine slides and send them to my Dr.* *We are at the beginning of our quest to place one in the lab.* *I am really interested in what my fellow colleagues opinions is concerning their own experience.* ** *Thanks* Stella W. Houston, Tx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cassette Printer Info Needed
Hello Everyone, The print head on the cassette printer needs to be replaced again. Informed that it will need to be replaced again in another 3 -4 months. This is something I am not looking forward to seeing repaired over and over again. This piece of equipment was made in 2010. Can anyone recommend the cassette writer that you are using ? Is this print head replacement normal with your unit ? Thanks Stella M-Walters ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Independent Equipment Service Companies
I lost the contact number I had for a company that will service histology equipment. Could you please give me some contact info. Thank You Stella ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Reagent Alc or Denatured Alc ?
What is the difference between the two ? Thinking about switching to denatured becasue of cost costing. Which do you prefer? Stella ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Microtome blades
I purchase Accu-edge blades from Stat Lab. Thanks to All for the comments on reagent and denatured alcohol. On Wed, Jan 9, 2013 at 2:00 PM, Hugh Luk hlu...@msn.com wrote: Laurie, Accuedge can also be found through: VWR (https://us.vwr.com/store/catalog/product.jsp?catalog_number=25608-964) or Electron Microscopy Services http://www.emsdiasum.com/microscopy/products/histology/sectioning.aspx I believe we're in a similar situation, as our institution has Favored vendors (volume discounts), so we had to look this up once or twice. Hope this helps, Hugh UH cancer center Date: Wed, 9 Jan 2013 09:13:55 -0700 From: Elizabeth Chlipala l...@premierlab.com Subject: [Histonet] RE: Microtome blades To: 'Laurie Colbert' lcolb...@pathmdlabs.com, Histonet Post (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Message-ID: 14E2C6176416974295479C64A11CB9AE016411ABF729@SBS2K8.premierlab.local Content-Type: text/plain; charset=us-ascii Mercedes Medical or directly from Sakura Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, January 09, 2013 8:56 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Microtome blades Does anyone know who I can purchase Accu-edge blades from, other than Cardinal or American Master Tech? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Nerve tease specimen holding
When I was doing nerve teasing, we would hold in glycerol in the refrig. On Thu, Jul 5, 2012 at 10:58 AM, Morken, Timothy timothy.mor...@ucsfmedctr.org wrote: For all you doing nerve teasing, do you hold the specimen in buffer (after fixation) until teasing is requested or go ahead and process through osmium and glycerol and then hold? Thanks! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.mor...@ucsfmedctr.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Need the name of a piece of equipment
Good day to all, I need the name of the equipment that melts the wax off the edges of a paraffin block. Where can I purchase it. Thank You Stella ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Independent Service Technician
Is there anyone out there that does repairs on Sakura equipment and works for themselves in the Texas area. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job Listing Houston,Tx
-- *ANNOUNCEMENT NUMBER: 14905- T* * * *JOB TITLE: Histology Technician II * * * *DEPARTMENT:Institute of Forensic Sciences* * * *HOURS: 7:30 a.m. – 4:40 p.m. / Flexible* *Monday - Friday* * * *SALARY:Commensurate With Experience* *Based On 26 Pay Periods* * * *EDUCATION: *Completion of an Associate’s degree and completion of histology school with histo-technician certification (ASCP) American Society of Clinical Pathologist *at the time of employment or within one year of employment. * * * *EXPERIENCE: *One year of experience in a histology laboratory in which responsibilities included production of stained slides and preservation of tissue samples in both paraffin and formalin is *required*.** * * *JOB SKILLS: *The successful applicant must have expertise in the use of microtomes, manual staining procedures, manual slide coverslipping, automated slide stainers, automated slide coverslipper, tissue processors and tissue embedding. Must be capable of understanding and adhering to strict protocols for the handling, trimming and archival of tissue samples and blocks; knowledge of histology laboratory safety rules and procedures *is essential*. Good interpersonal skills *are a must*. *JOB DESCRIPTION: *Prepares stained slides of autopsy tissues in a careful, controlled environment. Prepares paraffin blocks of tissue for long-term storage and labels the samples in accordance with histology laboratory protocols; assists with archival of paraffin-embedded and non-paraffin-embedded formalin fixed tissues. Provides all tissue slides to the assigned Assistant Medical Examiner, Deputy Chief Medical Examiner or Chief Medical Examiner on a timely basis; assists with data entry into a computerized database to track laboratory efficiency as required. Other job assignments as assigned by the Deputy Chief Medical Examiner. *Position requires a high level of confidentiality, responsibility and dependability.* *PHYSICAL REQUIREMENTS:** *Must be able to sit and stand for prolonged periods of time; able to lift up to 40lbs; stooping and bending may be required. * * *EMPLOYMENT IS CONTINGENT UPON PASSING A CRIMINAL BACKGROUND CHECK.* * * * * *HARRIS COUNTY HAS AN EMPLOYMENT AT WILL POLICY.* *CLOSING DATE: Open Until Filled* *APPLY AT:1310 PRAIRIE - SUITE 170* * * * * *UPON RECEIVING A CONDITIONAL OFFER OF EMPLOYMENT, ALL APPLICANTS ARE SCREENED FOR THE PRESENCE OF ILLEGAL DRUGS.*** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cutting speed
Teresa please express your concerns to your supervisor or lab manager now ! A new person in a lab needs more time than one month to get used to the routine feel of a new place. Did they use a PRN during their search for the new tech. If so, have that person come in and help during the vacation break of the the other tech. Our utmost concern should be care of the patient and safety of the tech. I know from personal experience that both will be compromised when you have a situation like yours. You will be rushed and tissue samples can be lost in embedding or cut away during microtomy. You could cut yourself also. I am not even taking into account the other responsibilities of a general histology lab that will be compromised as well. The histo students in our area are encouraged to be able to trim and cut 30 blocks in one hour. This does not include the time to embed the tissue, nor the time to stain and sign out the case. Worst case scenario; the other tech will have to delay the scheduled vacation until you are thoroughly acclimated to the flow of the lab. My personal opinion is that the this situation is totally unfair to any new employee, seasoned or new graduate. I would never do this to a new tech myself. Stay in our field, be encouraged, it does get better Teresa. Have a Great and Prosperous New Year Stella ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Manual embedding
Andrea, I have send you an idea that might work for you. Check your email. Hope this will help. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Manual embedding
A couple of ideas: Suggest looking for a used paraffin bath from an outside vendor. #2 Idea: If anyone knows of a lab that is closing up, they might be able to contact Andrea and pass that info. I know when a lab is closing up, storage of equipment becomes burdensome. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] How many tissues an histo tech is suppose to cut per
Joanne is not alone. The lab I work at has informed us that due to their own research, a single histotech should be able to do 100 blocks an hour. I'm not sure if they expect embedding, trimming, writing slides, sectioning and manual staining in this goal. I am a seasoned histotech, and have tried to speak to my lab manager and lab supervisor, (both are med. techs and have very little insight into what is involved in producing a high quality slide), but their goal remains the same. I have an idea: I feel like printing some of your responses to Joanne question and showing them that their goal is unattainable as well. Any other suggestions. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] How many tissues an histo tech is suppose to cut per
To All I Really Appreciate All The Great Info. I am fully armed and ready to present my case to management. The knowledge among everyone out there is incredible. Thanks. Stella ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Retirement
I am 9 yrs from retirement. Earlier once my boys quit going back to school. B.A. then M.A. now PH.D. I can scoop the place out for everyone, until you get there. My tan should be gorgeous by then. See you there. Stella On Fri, Jun 17, 2011 at 4:06 PM, Podawiltz, Thomas tpodawi...@lrgh.orgwrote: For our own commune, I would look at Bend, Oregon, nothing against northern CA it is so beautiful, buts its still CA. Plus the fact, I was born and raised in Oregon. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer From: histonet-boun...@lists.utsouthwestern.edu [ histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Garcia, Lori, Sr. Scientist [lori.gar...@medtronic.com] Sent: Friday, June 17, 2011 4:35 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retirement Count me in too! Somebody will have to be around for when the robots shut down or revolt and try to take over the world. I'm a midwesterner like Cindi, but have relocated to northern CA and it is paradise out here. We could get some land out in the boonies and start our own histo commune. Lori -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Friday, June 17, 2011 11:33 AM To: Cynthia Pyse; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retirement I have recently traveled back from the future, and it does not bode well for histologists. The robots are pretty reliable and can operate 14 hours on a single charge. They also do not have to wear safety glasses when working with the laser microtomes. No vacations, no calling in sick, no travelling to seminars (new software version upgrades come to them) I noticed that someone still has to come in in the morning to flip the switches, but since CLIA will not likely consider that testing Well, who'll need a histologist for that? Funny though, pathologists are still around, but they are all workiing in a single building near Area 72. (you don't want to know about area 72 unless you are still around in 2035, then EVERYONE will know about area 72) They spend their full 6 hour day looking at scanned images on big-screen monitors and drinking coffee. It's not that the future really needs them, but the AMA still lobbies for them more effectively than the ASCP for us. - Bill With apologies to all the really decent pathologists who monitor this forum. (I have to be nice because my only usable skill someday might be making a mean cup of coffee) -Original Message- From: Cynthia Pyse [mailto:cp...@x-celllab.com] Sent: Friday, June 17, 2011 12:51 PM To: O'Donnell, Bill; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retirement As long as we don't have to go back to steel knives, old AO micrtomes and the autotechnicon I'm in. I am also 15 to 18 years out. What is the world of histology going to do without us. Who will know how to make a solution of mucicarmine (not that I do anymore, but I could) or eosin? The tech I train now look at me like I am speaking a foreign language sometimes. Make me feel old, but closer to retirement. Cindy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Friday, June 17, 2011 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retirement OK, I know it is Friday, and I know that this may sound like a bit of a jokebut I am 15-18 years out from retirement and my wife and I want to retire someplace tropical And it would be smart to get settled in such a location. So, if anyone knows of any openings in Hawaii, Virgin Islands, St. Thomas, Puerto Rico for an experienced HT (ASCP) QIHC PLEASE let me know. Would be open to others, but would prefer a US territory. I can be reached at b...@deaconbill.com William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist/Safety Officer Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without
[Histonet] Americlear Staining Problems
Good Morning Histonetters, Our HE stained slides are bleeding out the eosin. No, I did not shoot the sheriff. Called the manufacturer, and was advised to: Give more time in the Alcohols, Use only 100% Alcohols after eosin. Change the last two Alcohols and Americlear. Done. Done. Done. Stain bleeding really improved, but still shows up on hit and miss cases. Has anyone had this problem and How did you fix it Stella ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Disposing of gross only items
Hello Everyone, Need some info on the disposal of gross only items. These items include hardware, wires, gallstones and foreign objects. We are running out of room for the storage of gross only specimens. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FT Job in Houston (Near Bush Airport)
We have a FT job opening for Hermann Hospital Northeast. Routine Histo work. Heavy bx's. Weekends off. Please contact the Hermann Hospital Website. Need more info - 281 540 6275 Thanks Stella ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Job - Houston (East Suburb)
FT Histo Job. Routine Work. Heavy Bx's Frozens, Special Stains Day Shift. Weekends off. Contact Herman Hospital Website - Northeast Location Further Info 281 540 6275 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PT Histology Job Houston, Tx (nights)
A group of endo. doctors are looking for one or two histotechs to gross, process, cut and stain tissues at their private lab, located in West Houston. The job will start late afternoon. If interested contact me . ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Floaters in Waterbath
I know we have all had some problems with floaters in our waterbath at some point in our microtomy career. Our doctors are very picky and I need some tips on keeping an immaculate clean waterbath, but not sacrificing the speed in a regular routine lab. We use the pyrex waterbath and paper towels for wiping our area. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: FW: [Histonet] Floaters in Waterbath
Thank You Fellow Histonetters, I greatly appreciate all the advice. Have a great week end. Stella On Fri, Oct 23, 2009 at 12:52 PM, Thomas Jasper tjas...@copc.net wrote: One more time. tj -Original Message- From: Thomas Jasper Sent: Friday, October 23, 2009 10:41 AM To: 'Stella Mireles' Subject: RE: [Histonet] Floaters in Waterbath Others may mention this to you...and it can get a little political. Do not discount the grossing bench. Whether it's the work of a PA, Pathologist, or other qualified lab staff, the grossing bench should be kept as clean as possible between cases/specimens. I mention the political side because sometimes it gets a bit touchy...histologists may not be in the best position to broach the subject with certain higher level personnel. Especially when the grossing may very well be done under the supervision of said higher level party. From a patient care standpoint, etc., this definitely should not be an ego-bruiser, as we are all human and make mistakes. But I'm sure most of you know what I'm talking about and probably have experienced something similar at sometime in your careers. One tip I learned from a pathologist, was to keep a clean sponge handy while grossing. This helped a lot, especially with keeping forceps etc., clean in between cases/specimens. Lastly, there are pathologists and PAs out there that keep their egos in check and we are thankful to them. Tom J. Thomas Jasper HT (ASCP) BAS Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Friday, October 23, 2009 7:11 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Floaters in Waterbath I know we have all had some problems with floaters in our waterbath at some point in our microtomy career. Our doctors are very picky and I need some tips on keeping an immaculate clean waterbath, but not sacrificing the speed in a regular routine lab. We use the pyrex waterbath and paper towels for wiping our area. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Alpha-naphthyl-butyrate
Looking for the order number for Alpha-naphthyl-butyrate. I will be using it for the NSE procedure. I spoke with the Fisher 800 number and they were unable to help me. We use Fisher or Sigma products. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Phosphofuctokinase Staining Procedure
Would anyone be willing to send me their procedure for Phosphofuctokinase, or a site where I can pull this up myself. The reference book that I was supplied with it not enough for me to pull what I need. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] EM Techs -RMC powertome ultramicrotome question
Is anyone using this ultramicrotome? I would like know how it compares to a Leica ultramicrotome? Thanks Stella ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] EM Knife
All EM Techs. Which Ultra knife is better. Diatome or Pelco? Is there any other company that I should consider. I plan to purchase an Ultra and a Histo knife. Thank You Stella ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet