Re: [Histonet] On-line references
John, Thank you for your response. I should have been a little clearer in my original post. I was posting the question on behalf of one of my students. In the course that I teach I have chosen to not use a required textbook. I list several recommended texts, including yours. Many of the students do in fact buy the texts. However, many use the texts I have reserved in our school's library. Since our library is closed, I wondered which current texts had e-versions so that the students could buy those and have access immediately. I was also wanting to direct our library to purchase e versions to prevent this kind of bottleneck from ever happening in the future. I am familiar with all of the common physical textbooks, but, not so for the electronic versions. I wondered if there were particularly good electronic versions. Thanks. Tom Tom Wells BSc, MEd, MLT, ART Faculty Medical Laboratory Science School of Health Sciences SW03-3088 (604) 412-7594 BCIT From: John Kiernan Sent: March 25, 2020 11:12 PM To: histonet@lists.utsouthwestern.edu; Tom Wells Subject: Re: On-line references CAUTION: This email originated from outside of BCIT. Do not click links or open attachments unless you recognize the sender and know the content is safe. Hello, Tom. Some old classics are there for free, most notably JR Baker's "Principles of Biological Microtechnique" (1958), but almost anything more recent has to be bought. There are plenty of cheap older editions of histotechnology books on sites like AbeBooks. Check it out for the last edition of Pearse's Histochemistry! I was amazed. Even the latest editions of books in our field cost only about $100 from the publisher and most are good for several years. Compare this with the price of a few drops of an antibody or (more realistically) a staining machine in which you must only use the liquids sold by its vendor. John Kiernan = = = ____ From: Tom Wells via Histonet mailto:histonet@lists.utsouthwestern.edu>> Sent: 25 March 2020 14:34 To: histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu> mailto:histonet@lists.utsouthwestern.edu>> Subject: [Histonet] On-line references Given that our Institute's library is closed due to the pandemic, is anyone aware of on-line versions of Histotechnology/ Histochemistry textbooks? Thanks. Tom Tom Wells BSc, MEd, MLT, ART Faculty Medical Laboratory Science School of Health Sciences SW03-3088 (604) 412-7594 BCIT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet<https://can01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet=02%7C01%7CTom_Wells%40bcit.ca%7C97a1afc359f94487995c08d7d14c887c%7C8322cefd0a4c4e2cbde5b17933e7b00f%7C0%7C0%7C637207999235053236=WS7oM8YSxGc65OPxcY%2BaZrRTRA%2Bmn7nkiw1GjRKiPvw%3D=0> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] On-line references
Thanks Bryan, Yes I refer to it all the time. I will add it to my list. Cheers. Tom Tom Wells BSc, MEd, MLT, ART Faculty Medical Laboratory Science School of Health Sciences SW03-3088 (604) 412-7594 BCIT -Original Message- From: Bryan Llewellyn Sent: March 25, 2020 1:28 PM To: Tom Wells ; Histonet Subject: Re: [Histonet] On-line references CAUTION: This email originated from outside of BCIT. Do not click links or open attachments unless you recognize the sender and know the content is safe. StainsFile is still available at https://can01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fstainsfile.infodata=02%7C01%7Ctom_wells%40bcit.ca%7C32033609df8e4a57bd9e08d7d0fd2638%7C8322cefd0a4c4e2cbde5b17933e7b00f%7C0%7C0%7C637207658731731714sdata=W1j3zzBIs5PibQUNjeY%2FcnOFBTy%2BshfBkvQOXPM1m%2BU%3Dreserved=0 In the downloads section there is a scanned, corrected and reformatted copy of the Microtomist's Vade Mecum, 7th edition. This text covers other areas as well as medical and is in the public domain in the US and Canada, and likely elsewhere. The problem with putting textbooks on line is that they must have their copyright expired and be in the public domain, which means they will invariably have been published in the first half of the 20th century, i.e. 50 or 60 years, or more, ago. Bryan Llewellyn Tom Wells via Histonet wrote: > Given that our Institute's library is closed due to the pandemic, is > anyone aware of on-line versions of Histotechnology/ Histochemistry > textbooks? Thanks. Tom > > Tom Wells BSc, MEd, MLT, ART > Faculty > Medical Laboratory Science > School of Health Sciences > SW03-3088 > (604) 412-7594 > BCIT > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://can01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists > .utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonetdata=02%7C01%7Ctom_wells%40bcit.ca%7C32033609df8e4a57bd9e08d7d0fd2638%7C8322cefd0a4c4e2cbde5b17933e7b00f%7C0%7C0%7C637207658731731714sdata=qtCSG4nto2RPGw9lD4ZmGhGg%2BMT2T260%2FTLvJB92YeA%3Dreserved=0 > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] On-line references
Given that our Institute's library is closed due to the pandemic, is anyone aware of on-line versions of Histotechnology/ Histochemistry textbooks? Thanks. Tom Tom Wells BSc, MEd, MLT, ART Faculty Medical Laboratory Science School of Health Sciences SW03-3088 (604) 412-7594 BCIT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] External Quality Assurance Programs
Hi, Some time ago I discovered an incredibly useful teaching resource. I believe it was part of an external quality assurance program for Histopathology. It had a large collection of images from materials that they had received for evaluation. These images were from all areas such as Immunohistochemistry, special stains, H, etc. They included ratings that they had given them as feedback. Including all mistakes that they had observed. Unfortunately, I didn't keep track of the site and I can no longer find it. If anyone can recall seeing this site I would appreciate it since it would take a lifetime to deliberately recreate all of the mistakes. Thanks. Tom Tom Wells BSc, MEd, MLT, ART Faculty Medical Laboratory Science School of Health Sciences SW03-3088 (604) 412-7594 BCIT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories
Dear Banjo, A reference to the article would be helpful; there must be more to it than one sentence! Formaldehyde has been known for decades to be hazardous, and there are safety regulations in places where it is used. Plenty of old-timers are still alive and well after woking with formaldehyde in the days when there were few or no regulations. I'm one of them. From about 1895 until about 1995 (and perhaps still, in some universities), every medical student spent most of the working day for at least a year with his or her nose and bare hands in a cadaver that had been embalmed in a cocktail containing phenol and formaldehyde. The predominant smell was the phenol, except when dissecting brains, which were fixed and stored in 4% formaldehyde. About 35 years ago, the American Association of Anatomists investigated effects of exposure to embalming chemicals on teachers of anatomy, who are in the dissecting room year after year. The only significant finding was eczema on the hands of some people, long known to be avoidable by wearing rubber gloves. Yes, I too should be able to provide a reference, but this was in the days of paper, which gets thrown out to make room for more paper ... There might be something deep in the archives at http://www.anatomy.org/ Other chemicals used in anatomy, pathology and histology labs also have their dangers; we avoid drinking them, rubbing them into our skin and inhaling their vapours, and we do our best to observe the safety regulations when it comes to getting rid of them. There is no substitute fixative functionally identical to formaldehyde. There are other fixatives, some less hazardous, but they have different effects on staining properties etc. The late Holde Puchtler published papers urging pathologists to use non-aqueous coagulant fixatives for routine fixation of small specimens, with her Carnoy variant methacarn (methanol 60, acetic acid 10, chloroform 30) as the probable best, also good for some modern molecular methods. For this I can provide a few references: Puchtler, H., Waldrop, F.S., Meloan, S.N., Terry, M.S. and Connor, H.M. (1970). Methacarn (methanol-Carnoy) fixation. Practical and theoretical considerations. Histochemie 21:97-116. Cox, M.L., Schray, C.L., Luster, C.N., Stewart, Z.S., Korytko, P.J., Khan, K.N.M., Paulauskis, J.D. and Dunstan, R.W. (2006). Assessment of fixatives, fixation and tissue processing on morphology and RNA integrity. Experimental and Molecular Pathology 80:183-191. Buesa, R.J. (2008). Histology without formalin? Annals of Diagnostic Pathology 12:387-396. Uneyama, C., Shibutani, M., Masutomi, N., Takagi, H. and Hirose, M. (2002). Methacarn fixation for genomic DNA analysis in microdissected paraffin-embedded tissue specimens. Journal of Histochemistry and Cytochemistry 50:1237-1245. Milcheva, R., Janega, P., Celec, P., Russev, R. and Babal, P. (2013). Alcohol based fixatives provide excellent tissue morphology, protein immunoreactivity and RNA integrity in paraffin embedded tissue specimens. Brain Research Protocols 115:279-289. Greer, C.E., Peterson, S.L., Kiviat, N.B. and Manos, M.M. (1991). PCR amplification from paraffin-embedded tissues. American Journal of Clinical Pathology 95:117-124. Tissue processing is extremely simple after non-aqueous coagulant fixation, and most of the stages of a processing machine are not needed. Nuclear chromatin details are much sharper than after formaldehyde. This may not be seen as a blessing by young and middle-aged pathologists. In bygone days the routine fixatives contained mercuric chloride, which gives crisp chromatin and cytoplasmic details. The heterochromatin details probably are artifacts of fixation, but they are useful for identifying cells. John Kiernan Old neuroanatomist and histochemist UWO, London, Canada http://publish.uwo.ca/~jkiernan/ Also Secretary, Biological Stain Commission http://biostain.com = = = On 13/07/15, Adesupo, Adesuyi (Banjo) wrote: Hi, I read this article (National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories) this morning. I wanted to know whether some of you guys out there are using Formaldehyde substitute. Best regards, Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 abades...@nrh-ok.com == CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error,
